MELO3C007870 (gene) Melon (DHL92) v4

BLAST of MELO3C007870 vs. NCBI nr
Match: KAA0025709.1 (3-epi-6-deoxocathasterone 23-monooxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 961.8 bits (2485), Expect = 2.2e-276
Identity = 475/485 (97.94%), Postives = 475/485 (97.94%), Query Frame = 0

Query: 1   MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60
           MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD
Sbjct: 1   MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60

Query: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120
           KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK
Sbjct: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120

Query: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV 180
           GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV
Sbjct: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV 180

Query: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240
           KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK
Sbjct: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240

Query: 241 SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC 300
           SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC
Sbjct: 241 SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC 300

Query: 301 PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360
           PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD
Sbjct: 301 PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360

Query: 361 VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEYGCNCKKYKQENLSATNNHN 420
           VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWE          ENLSATNNHN
Sbjct: 361 VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWE----------ENLSATNNHN 420

Query: 421 FTPFGGGQRLCPGVELTRLEISIFLHHLVTTYSQDEKEAANHSHNLKFLTGFNHLNNFDT 480
           FTPFGGGQRLCPGVELTRLEISIFLHHLVTTYSQDEKEAANHSHNLKFLTGFNHLNNFDT
Sbjct: 421 FTPFGGGQRLCPGVELTRLEISIFLHHLVTTYSQDEKEAANHSHNLKFLTGFNHLNNFDT 475

Query: 481 KLSRK 486
           KLSRK
Sbjct: 481 KLSRK 475

BLAST of MELO3C007870 vs. NCBI nr
Match: XP_008440770.1 (PREDICTED: 3-epi-6-deoxocathasterone 23-monooxygenase [Cucumis melo])

HSP 1 Score: 896.7 bits (2316), Expect = 8.6e-257
Identity = 446/468 (95.30%), Postives = 449/468 (95.94%), Query Frame = 0

Query: 1   MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60
           MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD
Sbjct: 1   MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60

Query: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120
           KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK
Sbjct: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120

Query: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV 180
           GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV
Sbjct: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV 180

Query: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240
           KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK
Sbjct: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240

Query: 241 SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC 300
           SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC
Sbjct: 241 SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC 300

Query: 301 PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360
           PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD
Sbjct: 301 PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360

Query: 361 VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEYGCNCKKYKQENLSATNNHN 420
           VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWE          ENLSATNNHN
Sbjct: 361 VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWE----------ENLSATNNHN 420

Query: 421 FTPFGGGQRLCPGVELTRLEISIFLHHLVTTYS-QDEKEAANHSHNLK 468
           FTPFGGGQRLCPGVELTRLEISIFLHHLVTTY  + EK+   H   +K
Sbjct: 421 FTPFGGGQRLCPGVELTRLEISIFLHHLVTTYRWEAEKDEIVHFPTVK 458

BLAST of MELO3C007870 vs. NCBI nr
Match: XP_004149982.1 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 [Cucumis sativus])

HSP 1 Score: 858.6 bits (2217), Expect = 2.6e-245
Identity = 430/452 (95.13%), Postives = 432/452 (95.58%), Query Frame = 0

Query: 1   MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60
           MG WV FVCVILGWFL KKKKKI+ENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD
Sbjct: 1   MGVWV-FVCVILGWFLLKKKKKIVENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60

Query: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120
           KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK
Sbjct: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120

Query: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV 180
           GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANW NNS LH QTEAKQITFEVLV
Sbjct: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANW-NNSQLHLQTEAKQITFEVLV 180

Query: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240
           KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK
Sbjct: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240

Query: 241 SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC 300
           S VGEKGAPRDAVDVLLQDH+ENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFL DC
Sbjct: 241 SMVGEKGAPRDAVDVLLQDHNENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLSDC 300

Query: 301 PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360
           PRALAQL EENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD
Sbjct: 301 PRALAQLKEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360

Query: 361 VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEYGCNCKKYKQENLSATNNHN 420
           VKIK YLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWE          ENLSATNNHN
Sbjct: 361 VKIKGYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWE----------ENLSATNNHN 420

Query: 421 FTPFGGGQRLCPGVELTRLEISIFLHHLVTTY 453
           FTPFGGGQRLCPGVELTRLEISIFLHHLVTTY
Sbjct: 421 FTPFGGGQRLCPGVELTRLEISIFLHHLVTTY 440

BLAST of MELO3C007870 vs. NCBI nr
Match: XP_038881879.1 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 [Benincasa hispida])

HSP 1 Score: 799.3 bits (2063), Expect = 1.9e-227
Identity = 399/470 (84.89%), Postives = 425/470 (90.43%), Query Frame = 0

Query: 1   MGFWVWFV-CVILGWFLFKKKKK-IIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTF 60
           +GFWVWFV  VILGWF  K+KKK  I N DGIP+GNLGWPFFGETLQFISSGYSSRPVTF
Sbjct: 5   LGFWVWFVSVVILGWFWLKRKKKNHIGNNDGIPRGNLGWPFFGETLQFISSGYSSRPVTF 64

Query: 61  MDKRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILR 120
           MDKRKSLYGNVFKTNILGTPIIVSTDSEVNKV+LQNHGNIF PAYPKSITELLGT+SILR
Sbjct: 65  MDKRKSLYGNVFKTNILGTPIIVSTDSEVNKVVLQNHGNIFIPAYPKSITELLGTFSILR 124

Query: 121 MKGNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEV 180
           M GNVQ+R+HT+IGAFLRSPQLKAQIT+DIQNTVQLRL+NWN++  ++ QTE KQITFEV
Sbjct: 125 MNGNVQKRVHTLIGAFLRSPQLKAQITQDIQNTVQLRLSNWNSSQLVYLQTETKQITFEV 184

Query: 181 LVKVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEE 240
           LVKVLMSVGPG+EL+ LKREFEEFIKALICIPIKLPGTRLYKSLKAR+KLLKIV KIVE+
Sbjct: 185 LVKVLMSVGPGEELKILKREFEEFIKALICIPIKLPGTRLYKSLKARKKLLKIVSKIVED 244

Query: 241 RKSRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLG 300
           RKSRV EK APRDAVDVLLQDH E QGLPLDFISSHIIELMIPGEETVP AMTLAVKFL 
Sbjct: 245 RKSRVEEKVAPRDAVDVLLQDHDEIQGLPLDFISSHIIELMIPGEETVPMAMTLAVKFLS 304

Query: 301 DCPRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQ 360
           DCPRALAQLMEENMKLKK+K+GSGEEY WTDYMSL FTQNVISETLRMANI+NGVWRKAQ
Sbjct: 305 DCPRALAQLMEENMKLKKQKNGSGEEYRWTDYMSLLFTQNVISETLRMANIINGVWRKAQ 364

Query: 361 KDVKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEYGCNCKKYKQENLSATNN 420
           KDV+IKDYLIPQGWCVLASFISVHMDEKNYANPH+FDPWRWE          ENLSATNN
Sbjct: 365 KDVRIKDYLIPQGWCVLASFISVHMDEKNYANPHQFDPWRWE----------ENLSATNN 424

Query: 421 HNFTPFGGGQRLCPGVELTRLEISIFLHHLVTTYSQD-EKEAANHSHNLK 468
           HNFTPFGGGQRLCPG+EL RLEISIFLHHLVTTY    EK+   H   +K
Sbjct: 425 HNFTPFGGGQRLCPGIELARLEISIFLHHLVTTYKWGAEKDEIIHFPTVK 464

BLAST of MELO3C007870 vs. NCBI nr
Match: KAE8647598.1 (hypothetical protein Csa_003166 [Cucumis sativus])

HSP 1 Score: 777.7 bits (2007), Expect = 5.8e-221
Identity = 388/400 (97.00%), Postives = 390/400 (97.50%), Query Frame = 0

Query: 1   MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60
           MG WV FVCVILGWFL KKKKKI+ENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD
Sbjct: 1   MGVWV-FVCVILGWFLLKKKKKIVENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60

Query: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120
           KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK
Sbjct: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120

Query: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV 180
           GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANW NNS LH QTEAKQITFEVLV
Sbjct: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANW-NNSQLHLQTEAKQITFEVLV 180

Query: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240
           KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK
Sbjct: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240

Query: 241 SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC 300
           S VGEKGAPRDAVDVLLQDH+ENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFL DC
Sbjct: 241 SMVGEKGAPRDAVDVLLQDHNENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLSDC 300

Query: 301 PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360
           PRALAQL EENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD
Sbjct: 301 PRALAQLKEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360

Query: 361 VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWE 401
           VKIK YLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWE
Sbjct: 361 VKIKGYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWE 398

BLAST of MELO3C007870 vs. ExPASy Swiss-Prot
Match: Q9M066 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 OS=Arabidopsis thaliana OX=3702 GN=CYP90C1 PE=1 SV=3)

HSP 1 Score: 591.7 bits (1524), Expect = 7.8e-168
Identity = 296/437 (67.73%), Postives = 348/437 (79.63%), Query Frame = 0

Query: 25  ENKDG-IPKGNLGWPFFGETLQFISSGYSSRPVTFMDKRKSLYGNVFKTNILGTPIIVST 84
           E K G IP G+LGWP  GETL FI+ GYSSRPVTFMDKRKSLYG VFKTNI+GTPII+ST
Sbjct: 63  EKKKGMIPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIIST 122

Query: 85  DSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMKGNVQRRLHTIIGAFLRSPQLKAQ 144
           D+EVNKV+LQNHGN F PAYPKSITELLG  SIL + G  Q+RLHT+IGAFLRSP LK +
Sbjct: 123 DAEVNKVVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDR 182

Query: 145 ITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLVKVLMSVGPGKELETLKREFEEFI 204
           IT+DI+ +V L LA+W     +H Q E K++TFE+LVKVLMS  PG+++  LK EFEEFI
Sbjct: 183 ITRDIEASVVLTLASWAQLPLVHVQDEIKKMTFEILVKVLMSTSPGEDMNILKLEFEEFI 242

Query: 205 KALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERKSRVGEKGAPRDAVDVLLQD--HS 264
           K LICIPIK PGTRLYKSLKA+E+L+K+V K+VEER+  +       D VDVLL+D   S
Sbjct: 243 KGLICIPIKFPGTRLYKSLKAKERLIKMVKKVVEERQVAMTTTSPANDVVDVLLRDGGDS 302

Query: 265 ENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDCPRALAQLMEENMKLKKEKDGS 324
           E Q  P DF+S  I+E+MIPGEET+PTAMTLAVKFL D P ALA+L+EENM++K+ K   
Sbjct: 303 EKQSQPSDFVSGKIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKLVEENMEMKRRKLEL 362

Query: 325 GEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKDVKIKDYLIPQGWCVLASFISV 384
           GEEY WTDYMSL FTQNVI+ETLRMANI+NGVWRKA KDV+IK YLIP+GWCVLASFISV
Sbjct: 363 GEEYKWTDYMSLSFTQNVINETLRMANIINGVWRKALKDVEIKGYLIPKGWCVLASFISV 422

Query: 385 HMDEKNYANPHEFDPWRWEYGCNCKKYKQENLSATNNHNFTPFGGGQRLCPGVELTRLEI 444
           HMDE  Y NP++FDPWRW+         + N SA ++  FTPFGGGQRLCPG+EL++LEI
Sbjct: 423 HMDEDIYDNPYQFDPWRWD---------RINGSANSSICFTPFGGGQRLCPGLELSKLEI 482

Query: 445 SIFLHHLVTTYSQDEKE 459
           SIFLHHLVT YS   +E
Sbjct: 483 SIFLHHLVTRYSWTAEE 490

BLAST of MELO3C007870 vs. ExPASy Swiss-Prot
Match: Q94IW5 (Cytochrome P450 90D2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90D2 PE=1 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 9.9e-123
Identity = 212/442 (47.96%), Postives = 317/442 (71.72%), Query Frame = 0

Query: 30  IPKGNLGWPFFGETLQFISSGYSSRPVTFMDKRKSLYGN-VFKTNILGTPIIVSTDSEVN 89
           +P G+ GWP  GETL+F+S  YS RP  F+DKR+ L+G+ VF++++ G+  +V+ D+EV+
Sbjct: 49  LPPGSFGWPVVGETLEFVSCAYSPRPEAFVDKRRKLHGSAVFRSHLFGSATVVTADAEVS 108

Query: 90  KVILQNHGNIFTPAYPKSITELLGTYSILRMKGNVQRRLHTIIGAFLRSPQLKAQITKDI 149
           + +LQ+    F P YP+S+TEL+G  SIL + G +QRR+H ++GAF +S  LK+Q+T D+
Sbjct: 109 RFVLQSDARAFVPWYPRSLTELMGKSSILLINGALQRRVHGLVGAFFKSSHLKSQLTADM 168

Query: 150 QNTVQLRLANWNNNSPLHFQTEAKQITFEVLVKVLMSVGPGKELETLKREFEEFIKALIC 209
           +  +   L+++ ++S LH Q  AK + FE+LV+ L+ +  G+E++ LK++F+EFI  L+ 
Sbjct: 169 RRRLSPALSSFPDSSLLHVQHLAKSVVFEILVRGLIGLEAGEEMQQLKQQFQEFIVGLMS 228

Query: 210 IPIKLPGTRLYKSLKAREKLLKIVGKIVEERKSRVGEKGAPRDAVDVLLQDHSENQGLPL 269
           +PIKLPGTRLY+SL+A++K+ +++ +I+ E+++R      PRDA+DVL+ D S+   L  
Sbjct: 229 LPIKLPGTRLYRSLQAKKKMARLIQRIIREKRARRAAASPPRDAIDVLIGDGSDE--LTD 288

Query: 270 DFISSHIIELMIPGEETVPTAMTLAVKFLGDCPRALAQLMEENMKLKKEKDGSGEEYTWT 329
           + IS ++I+LMIP E++VP  +TLAVKFL +CP AL QL EEN++LK+ K   GE   WT
Sbjct: 289 ELISDNMIDLMIPAEDSVPVLITLAVKFLSECPLALHQLEEENIQLKRRKTDMGETLQWT 348

Query: 330 DYMSLQFTQNVISETLRMANIVNGVWRKAQKDVKIKDYLIPQGWCVLASFISVHMDEKNY 389
           DYMSL FTQ+VI+ETLR+ NI+ G+ RKA +DV++K +LIP+GWCV   F SVH+D+  Y
Sbjct: 349 DYMSLSFTQHVITETLRLGNIIGGIMRKAVRDVEVKGHLIPKGWCVFVYFRSVHLDDTLY 408

Query: 390 ANPHEFDPWRWEYGCNCKKYKQENLSATNNHNFTPFGGGQRLCPGVELTRLEISIFLHHL 449
             P++F+PWRW         K++++S   N +FTPFGGGQRLCPG++L RLE SIFLHHL
Sbjct: 409 DEPYKFNPWRW---------KEKDMS---NGSFTPFGGGQRLCPGLDLARLEASIFLHHL 468

Query: 450 VTTYSQDEKEAANHSHNLKFLT 471
           VT++    +  A   H + F T
Sbjct: 469 VTSF----RWVAEEDHIVNFPT 472

BLAST of MELO3C007870 vs. ExPASy Swiss-Prot
Match: Q94IA6 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 OS=Arabidopsis thaliana OX=3702 GN=CYP90D1 PE=1 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 5.5e-121
Identity = 212/426 (49.77%), Postives = 299/426 (70.19%), Query Frame = 0

Query: 31  PKGNLGWPFFGETLQFISSGYSSRPVTFMDKRKSLYGNVFKTNILGTPIIVSTDSEVNKV 90
           P G+LGWP  GET++F+SS YS RP +FMDKR+ +YG VFK++I GT  IVSTD+EVN+ 
Sbjct: 52  PHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTDAEVNRA 111

Query: 91  ILQNHGNIFTPAYPKSITELLGTYSILRMKGNVQRRLHTIIGAFLRSPQLKAQITKDIQN 150
           +LQ+    F P YPK++ EL+G  SIL + G++ RR H ++G+FL+SP LKAQI +D+  
Sbjct: 112 VLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRDMHK 171

Query: 151 TVQLRLANWNNNSPLHFQTEAKQITFEVLVKVLMSVGPGKELETLKREFEEFIKALICIP 210
            +   +  W+ + P+  Q  +K + F+VL K L+SV  G++LE LKREFE FI  L+ +P
Sbjct: 172 FLSESMDLWSEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFENFISGLMSLP 231

Query: 211 IKLPGTRLYKSLKAREKLLKIVGKIVE----ERKSRVGEKGAPRDAVDVLLQDHSENQGL 270
           I  PGT+L++SL+A++ ++K V +I+E    + K++  +    +D VDVLL+D SE+  L
Sbjct: 232 INFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVLLKDSSEH--L 291

Query: 271 PLDFISSHIIELMIPGEETVPTAMTLAVKFLGDCPRALAQLMEENMKLKKEKDGSGEEYT 330
             + I++++I++MIPG ++VP  +TLAVKFL D P AL  L EENMKLK  K+ +GE   
Sbjct: 292 THNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLKSLKELTGEPLY 351

Query: 331 WTDYMSLQFTQNVISETLRMANIVNGVWRKAQKDVKIKDYLIPQGWCVLASFISVHMDEK 390
           W DY+SL FTQ VI+ETLRM N++ GV RKA KDV+IK Y+IP+GWC LA   SVH+D+ 
Sbjct: 352 WNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCFLAYLRSVHLDKL 411

Query: 391 NYANPHEFDPWRWEYGCNCKKYKQENLSATNNHNFTPFGGGQRLCPGVELTRLEISIFLH 450
            Y +P++F+PWRW+                N  +F+PFGGGQRLCPG++L RLE S+FLH
Sbjct: 412 YYESPYKFNPWRWQE------------RDMNTSSFSPFGGGQRLCPGLDLARLETSVFLH 463

Query: 451 HLVTTY 453
           HLVT +
Sbjct: 472 HLVTRF 463

BLAST of MELO3C007870 vs. ExPASy Swiss-Prot
Match: Q42569 (Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 5.0e-90
Identity = 180/426 (42.25%), Postives = 262/426 (61.50%), Query Frame = 0

Query: 29  GIPKGNLGWPFFGETLQFISSGYSSRPVTFMDKRKSLYGNVFKTNILGTPIIVSTDSEVN 88
           G+P G+LG P  GET Q I +  +  P  F+D+R + YG+VF T++ G P I S D E N
Sbjct: 30  GLPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETN 89

Query: 89  KVILQNHGNIFTPAYPKSITELLGTYSILRMKGNVQRRLHTIIGAFLRSPQLKAQITKDI 148
           + +LQN G +F  +YP SI  LLG +S+L MKG++ +R+H++  +F  S  +K  +  DI
Sbjct: 90  RFVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDI 149

Query: 149 QNTVQLRLANWNNNSPLHFQTEAKQITFEVLVKVLMSVGPGKELETLKREFEEFIKALIC 208
              V+  L +W  +S +    EAK+ITFE+ VK LMS  PG+  E+L++E+   I+    
Sbjct: 150 DRLVRFNLDSW--SSRVLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFS 209

Query: 209 IPIKLPGTRLYKSLKAREKLLKIVGKIVEERKSRVGEKGAPRDAVDVLLQDHSENQGLPL 268
           +P+ L  T   K+++AR K+ + +  +V +R+    E+GA R   D+L    + + G   
Sbjct: 210 LPLPLFSTTYRKAIQARRKVAEALTVVVMKRREE-EEEGAERKK-DMLAALLAADDGFSD 269

Query: 269 DFISSHIIELMIPGEETVPTAMTLAVKFLGDCPRALAQLMEENMKLKKEKDGSGEEYTWT 328
           + I   ++ L++ G ET  T MTLAVKFL + P ALAQL EE+ K++  K  S     W+
Sbjct: 270 EEIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSDS-YSLEWS 329

Query: 329 DYMSLQFTQNVISETLRMANIVNGVWRKAQKDVKIKDYLIPQGWCVLASFISVHMDEKNY 388
           DY S+ FTQ V++ETLR+ANI+ GV+R+A  DV+IK Y IP+GW V +SF +VH+D  ++
Sbjct: 330 DYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHLDPNHF 389

Query: 389 ANPHEFDPWRWEYGCNCKKYKQENLSATNNHN-FTPFGGGQRLCPGVELTRLEISIFLHH 448
            +   F+PWRW          Q N   T   N FTPFGGG RLCPG EL R+ +S+FLH 
Sbjct: 390 KDARTFNPWRW----------QSNSVTTGPSNVFTPFGGGPRLCPGYELARVALSVFLHR 440

Query: 449 LVTTYS 454
           LVT +S
Sbjct: 450 LVTGFS 440

BLAST of MELO3C007870 vs. ExPASy Swiss-Prot
Match: O64989 (Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2)

HSP 1 Score: 323.6 bits (828), Expect = 3.9e-87
Identity = 170/466 (36.48%), Postives = 265/466 (56.87%), Query Frame = 0

Query: 15  FLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMDKRKSLYGNVFKTNI 74
           FL   K++  + +  +P G  GWPF GET+ ++    ++    FM +  S YG ++++N+
Sbjct: 24  FLILLKRRNRKTRFNLPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHVSKYGKIYRSNL 83

Query: 75  LGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMKGNVQRRLHTIIGAF 134
            G P IVS D+ +N+ ILQN G +F  +YP+SI  +LG +S+L + G++ R + +I   F
Sbjct: 84  FGEPTIVSADAGLNRFILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSISLNF 143

Query: 135 LRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLVKVLMSVGPG-KELE 194
           L   +L+  + KD++      L +W  NS    Q EAK+ TF ++ K +MS+ PG +E E
Sbjct: 144 LSHARLRTILLKDVERHTLFVLDSWQQNSIFSAQDEAKKFTFNLMAKHIMSMDPGEEETE 203

Query: 195 TLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERKSRVGEKG------ 254
            LK+E+  F+K ++  P+ LPGT  +K+L++R  +LK + + +EERK  + E+       
Sbjct: 204 QLKKEYVTFMKGVVSAPLNLPGTAYHKALQSRATILKFIERKMEERKLDIKEEDQEEEEV 263

Query: 255 APRDAVDVLLQDHSENQ--------------GLPLDFISSHIIELMIPGEETVPTAMTLA 314
              D  ++   DH   Q               L  + I   I+ L+  G ET   A+ LA
Sbjct: 264 KTEDEAEMSKSDHVRKQRTDDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSVAIALA 323

Query: 315 VKFLGDCPRALAQLMEENMKLKKEKDGSGE-EYTWTDYMSLQFTQNVISETLRMANIVNG 374
           + FL  CP+A+ +L EE++++ + K   GE E  W DY  + FTQ VI+ETLR+ N+V  
Sbjct: 324 IFFLQACPKAVEELREEHLEIARAKKELGESELNWDDYKKMDFTQCVINETLRLGNVVRF 383

Query: 375 VWRKAQKDVKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEYGCNCKKYKQEN 434
           + RKA KDV+ K Y IP GW VL    +VH+D   Y  P+ F+PWRW+   N        
Sbjct: 384 LHRKALKDVRYKGYDIPSGWKVLPVISAVHLDNSRYDQPNLFNPWRWQQQNNGASSSGSG 443

Query: 435 LSATNNHNFTPFGGGQRLCPGVELTRLEISIFLHHLVTTYSQDEKE 459
             +T  +N+ PFGGG RLC G EL +LE+++F+HHLV  ++ +  E
Sbjct: 444 SFSTWGNNYMPFGGGPRLCAGSELAKLEMAVFIHHLVLKFNWELAE 489

BLAST of MELO3C007870 vs. ExPASy TrEMBL
Match: A0A5A7SKB2 (3-epi-6-deoxocathasterone 23-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold253G002030 PE=3 SV=1)

HSP 1 Score: 961.8 bits (2485), Expect = 1.1e-276
Identity = 475/485 (97.94%), Postives = 475/485 (97.94%), Query Frame = 0

Query: 1   MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60
           MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD
Sbjct: 1   MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60

Query: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120
           KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK
Sbjct: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120

Query: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV 180
           GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV
Sbjct: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV 180

Query: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240
           KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK
Sbjct: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240

Query: 241 SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC 300
           SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC
Sbjct: 241 SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC 300

Query: 301 PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360
           PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD
Sbjct: 301 PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360

Query: 361 VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEYGCNCKKYKQENLSATNNHN 420
           VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWE          ENLSATNNHN
Sbjct: 361 VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWE----------ENLSATNNHN 420

Query: 421 FTPFGGGQRLCPGVELTRLEISIFLHHLVTTYSQDEKEAANHSHNLKFLTGFNHLNNFDT 480
           FTPFGGGQRLCPGVELTRLEISIFLHHLVTTYSQDEKEAANHSHNLKFLTGFNHLNNFDT
Sbjct: 421 FTPFGGGQRLCPGVELTRLEISIFLHHLVTTYSQDEKEAANHSHNLKFLTGFNHLNNFDT 475

Query: 481 KLSRK 486
           KLSRK
Sbjct: 481 KLSRK 475

BLAST of MELO3C007870 vs. ExPASy TrEMBL
Match: A0A1S3B2I5 (3-epi-6-deoxocathasterone 23-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103485087 PE=3 SV=1)

HSP 1 Score: 896.7 bits (2316), Expect = 4.2e-257
Identity = 446/468 (95.30%), Postives = 449/468 (95.94%), Query Frame = 0

Query: 1   MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60
           MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD
Sbjct: 1   MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60

Query: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120
           KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK
Sbjct: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120

Query: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV 180
           GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV
Sbjct: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV 180

Query: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240
           KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK
Sbjct: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240

Query: 241 SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC 300
           SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC
Sbjct: 241 SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC 300

Query: 301 PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360
           PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD
Sbjct: 301 PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360

Query: 361 VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEYGCNCKKYKQENLSATNNHN 420
           VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWE          ENLSATNNHN
Sbjct: 361 VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWE----------ENLSATNNHN 420

Query: 421 FTPFGGGQRLCPGVELTRLEISIFLHHLVTTYS-QDEKEAANHSHNLK 468
           FTPFGGGQRLCPGVELTRLEISIFLHHLVTTY  + EK+   H   +K
Sbjct: 421 FTPFGGGQRLCPGVELTRLEISIFLHHLVTTYRWEAEKDEIVHFPTVK 458

BLAST of MELO3C007870 vs. ExPASy TrEMBL
Match: A0A0A0KH50 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G504590 PE=3 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 3.8e-234
Identity = 415/452 (91.81%), Postives = 418/452 (92.48%), Query Frame = 0

Query: 1   MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60
           MG WV FVCVILGWFL KKKKKI+ENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD
Sbjct: 1   MGVWV-FVCVILGWFLLKKKKKIVENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60

Query: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120
           KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK
Sbjct: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120

Query: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV 180
           GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANW NNS LH QTEAKQITFEVLV
Sbjct: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANW-NNSQLHLQTEAKQITFEVLV 180

Query: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240
           KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK
Sbjct: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240

Query: 241 SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC 300
           S VGEKGAPRDAVDVLLQDH+ENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFL DC
Sbjct: 241 SMVGEKGAPRDAVDVLLQDHNENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLSDC 300

Query: 301 PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360
           PRALAQL               +EYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD
Sbjct: 301 PRALAQL---------------KEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360

Query: 361 VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEYGCNCKKYKQENLSATNNHN 420
           VKIK YLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWE          ENLSATNNHN
Sbjct: 361 VKIKGYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWE----------ENLSATNNHN 420

Query: 421 FTPFGGGQRLCPGVELTRLEISIFLHHLVTTY 453
           FTPFGGGQRLCPGVELTRLEISIFLHHLVTTY
Sbjct: 421 FTPFGGGQRLCPGVELTRLEISIFLHHLVTTY 425

BLAST of MELO3C007870 vs. ExPASy TrEMBL
Match: A0A6J1BUA0 (3-epi-6-deoxocathasterone 23-monooxygenase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005423 PE=3 SV=1)

HSP 1 Score: 722.6 bits (1864), Expect = 1.1e-204
Identity = 362/470 (77.02%), Postives = 406/470 (86.38%), Query Frame = 0

Query: 1   MGFWVWFVCVILGWFLFKKKKKIIENKDG-IPKGNLGWPFFGETLQFISSGYSSRPVTFM 60
           +GFWV F+  +LG + +  K + I+NKDG IPKGNLGWPF GETLQFI+SGYSSRPVTFM
Sbjct: 7   LGFWVCFLSAVLGSWFWMNKMRNIDNKDGRIPKGNLGWPFIGETLQFIASGYSSRPVTFM 66

Query: 61  DKRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRM 120
           DKRKSLYG VFKTNILGTPIIVSTDSEVNKV+LQNHGNIF PAYPKSITELLG +SIL+M
Sbjct: 67  DKRKSLYGEVFKTNILGTPIIVSTDSEVNKVVLQNHGNIFIPAYPKSITELLGPFSILQM 126

Query: 121 KGNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVL 180
            GNVQR++H+IIGAFLRSP LK++ITKDI NTV+L LANWN +  ++ QT+ KQITFEVL
Sbjct: 127 NGNVQRKVHSIIGAFLRSPLLKSRITKDIHNTVRLSLANWNPSHLVYVQTQTKQITFEVL 186

Query: 181 VKVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEER 240
           VKVLMSVGPG++L+ LKREFEEFIK LICIP+KLPGTRLYKSLKAREKLLKIV KIVEE+
Sbjct: 187 VKVLMSVGPGEDLKILKREFEEFIKGLICIPLKLPGTRLYKSLKAREKLLKIVRKIVEEK 246

Query: 241 KSRVGEKGAP-RDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLG 300
           K  + EK AP +DAV+VLL+DH  +QGLPLDFISSH+IELMIPGEETVP AMTLAVKFL 
Sbjct: 247 KWAMAEKAAPAKDAVEVLLEDHEGSQGLPLDFISSHVIELMIPGEETVPMAMTLAVKFLS 306

Query: 301 DCPRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQ 360
           D P ALAQ MEEN +LKK+KD SGEEYTWTD+MSLQFTQNVISETLRMANI+NGVWRKAQ
Sbjct: 307 DSPVALAQTMEENKELKKQKDSSGEEYTWTDFMSLQFTQNVISETLRMANIINGVWRKAQ 366

Query: 361 KDVKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEYGCNCKKYKQENLSATNN 420
           KDV+IK YLIPQGWCVLASFISVHMDE+NYANPH+F+PWRWE          E  SATNN
Sbjct: 367 KDVEIKGYLIPQGWCVLASFISVHMDEENYANPHQFNPWRWE----------EIGSATNN 426

Query: 421 HNFTPFGGGQRLCPGVELTRLEISIFLHHLVTTYS-QDEKEAANHSHNLK 468
           HNFTPFGGGQRLCPG+EL RLEISIFLH LVTTY  + EK+   H   +K
Sbjct: 427 HNFTPFGGGQRLCPGIELARLEISIFLHCLVTTYKWRAEKDEIIHFPTVK 466

BLAST of MELO3C007870 vs. ExPASy TrEMBL
Match: A0A5D3CNC1 (3-epi-6-deoxocathasterone 23-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G001660 PE=3 SV=1)

HSP 1 Score: 717.6 bits (1851), Expect = 3.5e-203
Identity = 357/367 (97.28%), Postives = 357/367 (97.28%), Query Frame = 0

Query: 119 MKGNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEV 178
           MKGNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEV
Sbjct: 1   MKGNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEV 60

Query: 179 LVKVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEE 238
           LVKVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEE
Sbjct: 61  LVKVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEE 120

Query: 239 RKSRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLG 298
           RKSRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLG
Sbjct: 121 RKSRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLG 180

Query: 299 DCPRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQ 358
           DCPRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQ
Sbjct: 181 DCPRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQ 240

Query: 359 KDVKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEYGCNCKKYKQENLSATNN 418
           KDVKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWE          ENLSATNN
Sbjct: 241 KDVKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWE----------ENLSATNN 300

Query: 419 HNFTPFGGGQRLCPGVELTRLEISIFLHHLVTTYSQDEKEAANHSHNLKFLTGFNHLNNF 478
           HNFTPFGGGQRLCPGVELTRLEISIFLHHLVTTYSQDEKEAANHSHNLKFLTGFNHLNNF
Sbjct: 301 HNFTPFGGGQRLCPGVELTRLEISIFLHHLVTTYSQDEKEAANHSHNLKFLTGFNHLNNF 357

Query: 479 DTKLSRK 486
           DTKLSRK
Sbjct: 361 DTKLSRK 357

BLAST of MELO3C007870 vs. TAIR 10
Match: AT4G36380.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 591.7 bits (1524), Expect = 5.5e-169
Identity = 296/437 (67.73%), Postives = 348/437 (79.63%), Query Frame = 0

Query: 25  ENKDG-IPKGNLGWPFFGETLQFISSGYSSRPVTFMDKRKSLYGNVFKTNILGTPIIVST 84
           E K G IP G+LGWP  GETL FI+ GYSSRPVTFMDKRKSLYG VFKTNI+GTPII+ST
Sbjct: 63  EKKKGMIPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIIST 122

Query: 85  DSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMKGNVQRRLHTIIGAFLRSPQLKAQ 144
           D+EVNKV+LQNHGN F PAYPKSITELLG  SIL + G  Q+RLHT+IGAFLRSP LK +
Sbjct: 123 DAEVNKVVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDR 182

Query: 145 ITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLVKVLMSVGPGKELETLKREFEEFI 204
           IT+DI+ +V L LA+W     +H Q E K++TFE+LVKVLMS  PG+++  LK EFEEFI
Sbjct: 183 ITRDIEASVVLTLASWAQLPLVHVQDEIKKMTFEILVKVLMSTSPGEDMNILKLEFEEFI 242

Query: 205 KALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERKSRVGEKGAPRDAVDVLLQD--HS 264
           K LICIPIK PGTRLYKSLKA+E+L+K+V K+VEER+  +       D VDVLL+D   S
Sbjct: 243 KGLICIPIKFPGTRLYKSLKAKERLIKMVKKVVEERQVAMTTTSPANDVVDVLLRDGGDS 302

Query: 265 ENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDCPRALAQLMEENMKLKKEKDGS 324
           E Q  P DF+S  I+E+MIPGEET+PTAMTLAVKFL D P ALA+L+EENM++K+ K   
Sbjct: 303 EKQSQPSDFVSGKIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKLVEENMEMKRRKLEL 362

Query: 325 GEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKDVKIKDYLIPQGWCVLASFISV 384
           GEEY WTDYMSL FTQNVI+ETLRMANI+NGVWRKA KDV+IK YLIP+GWCVLASFISV
Sbjct: 363 GEEYKWTDYMSLSFTQNVINETLRMANIINGVWRKALKDVEIKGYLIPKGWCVLASFISV 422

Query: 385 HMDEKNYANPHEFDPWRWEYGCNCKKYKQENLSATNNHNFTPFGGGQRLCPGVELTRLEI 444
           HMDE  Y NP++FDPWRW+         + N SA ++  FTPFGGGQRLCPG+EL++LEI
Sbjct: 423 HMDEDIYDNPYQFDPWRWD---------RINGSANSSICFTPFGGGQRLCPGLELSKLEI 482

Query: 445 SIFLHHLVTTYSQDEKE 459
           SIFLHHLVT YS   +E
Sbjct: 483 SIFLHHLVTRYSWTAEE 490

BLAST of MELO3C007870 vs. TAIR 10
Match: AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )

HSP 1 Score: 436.0 bits (1120), Expect = 3.9e-122
Identity = 212/426 (49.77%), Postives = 299/426 (70.19%), Query Frame = 0

Query: 31  PKGNLGWPFFGETLQFISSGYSSRPVTFMDKRKSLYGNVFKTNILGTPIIVSTDSEVNKV 90
           P G+LGWP  GET++F+SS YS RP +FMDKR+ +YG VFK++I GT  IVSTD+EVN+ 
Sbjct: 52  PHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTDAEVNRA 111

Query: 91  ILQNHGNIFTPAYPKSITELLGTYSILRMKGNVQRRLHTIIGAFLRSPQLKAQITKDIQN 150
           +LQ+    F P YPK++ EL+G  SIL + G++ RR H ++G+FL+SP LKAQI +D+  
Sbjct: 112 VLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRDMHK 171

Query: 151 TVQLRLANWNNNSPLHFQTEAKQITFEVLVKVLMSVGPGKELETLKREFEEFIKALICIP 210
            +   +  W+ + P+  Q  +K + F+VL K L+SV  G++LE LKREFE FI  L+ +P
Sbjct: 172 FLSESMDLWSEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFENFISGLMSLP 231

Query: 211 IKLPGTRLYKSLKAREKLLKIVGKIVE----ERKSRVGEKGAPRDAVDVLLQDHSENQGL 270
           I  PGT+L++SL+A++ ++K V +I+E    + K++  +    +D VDVLL+D SE+  L
Sbjct: 232 INFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVLLKDSSEH--L 291

Query: 271 PLDFISSHIIELMIPGEETVPTAMTLAVKFLGDCPRALAQLMEENMKLKKEKDGSGEEYT 330
             + I++++I++MIPG ++VP  +TLAVKFL D P AL  L EENMKLK  K+ +GE   
Sbjct: 292 THNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLKSLKELTGEPLY 351

Query: 331 WTDYMSLQFTQNVISETLRMANIVNGVWRKAQKDVKIKDYLIPQGWCVLASFISVHMDEK 390
           W DY+SL FTQ VI+ETLRM N++ GV RKA KDV+IK Y+IP+GWC LA   SVH+D+ 
Sbjct: 352 WNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCFLAYLRSVHLDKL 411

Query: 391 NYANPHEFDPWRWEYGCNCKKYKQENLSATNNHNFTPFGGGQRLCPGVELTRLEISIFLH 450
            Y +P++F+PWRW+                N  +F+PFGGGQRLCPG++L RLE S+FLH
Sbjct: 412 YYESPYKFNPWRWQE------------RDMNTSSFSPFGGGQRLCPGLDLARLETSVFLH 463

Query: 451 HLVTTY 453
           HLVT +
Sbjct: 472 HLVTRF 463

BLAST of MELO3C007870 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 333.2 bits (853), Expect = 3.5e-91
Identity = 180/426 (42.25%), Postives = 262/426 (61.50%), Query Frame = 0

Query: 29  GIPKGNLGWPFFGETLQFISSGYSSRPVTFMDKRKSLYGNVFKTNILGTPIIVSTDSEVN 88
           G+P G+LG P  GET Q I +  +  P  F+D+R + YG+VF T++ G P I S D E N
Sbjct: 30  GLPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETN 89

Query: 89  KVILQNHGNIFTPAYPKSITELLGTYSILRMKGNVQRRLHTIIGAFLRSPQLKAQITKDI 148
           + +LQN G +F  +YP SI  LLG +S+L MKG++ +R+H++  +F  S  +K  +  DI
Sbjct: 90  RFVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDI 149

Query: 149 QNTVQLRLANWNNNSPLHFQTEAKQITFEVLVKVLMSVGPGKELETLKREFEEFIKALIC 208
              V+  L +W  +S +    EAK+ITFE+ VK LMS  PG+  E+L++E+   I+    
Sbjct: 150 DRLVRFNLDSW--SSRVLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFS 209

Query: 209 IPIKLPGTRLYKSLKAREKLLKIVGKIVEERKSRVGEKGAPRDAVDVLLQDHSENQGLPL 268
           +P+ L  T   K+++AR K+ + +  +V +R+    E+GA R   D+L    + + G   
Sbjct: 210 LPLPLFSTTYRKAIQARRKVAEALTVVVMKRREE-EEEGAERKK-DMLAALLAADDGFSD 269

Query: 269 DFISSHIIELMIPGEETVPTAMTLAVKFLGDCPRALAQLMEENMKLKKEKDGSGEEYTWT 328
           + I   ++ L++ G ET  T MTLAVKFL + P ALAQL EE+ K++  K  S     W+
Sbjct: 270 EEIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSDS-YSLEWS 329

Query: 329 DYMSLQFTQNVISETLRMANIVNGVWRKAQKDVKIKDYLIPQGWCVLASFISVHMDEKNY 388
           DY S+ FTQ V++ETLR+ANI+ GV+R+A  DV+IK Y IP+GW V +SF +VH+D  ++
Sbjct: 330 DYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHLDPNHF 389

Query: 389 ANPHEFDPWRWEYGCNCKKYKQENLSATNNHN-FTPFGGGQRLCPGVELTRLEISIFLHH 448
            +   F+PWRW          Q N   T   N FTPFGGG RLCPG EL R+ +S+FLH 
Sbjct: 390 KDARTFNPWRW----------QSNSVTTGPSNVFTPFGGGPRLCPGYELARVALSVFLHR 440

Query: 449 LVTTYS 454
           LVT +S
Sbjct: 450 LVTGFS 440

BLAST of MELO3C007870 vs. TAIR 10
Match: AT3G50660.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 323.6 bits (828), Expect = 2.8e-88
Identity = 170/466 (36.48%), Postives = 265/466 (56.87%), Query Frame = 0

Query: 15  FLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMDKRKSLYGNVFKTNI 74
           FL   K++  + +  +P G  GWPF GET+ ++    ++    FM +  S YG ++++N+
Sbjct: 24  FLILLKRRNRKTRFNLPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHVSKYGKIYRSNL 83

Query: 75  LGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMKGNVQRRLHTIIGAF 134
            G P IVS D+ +N+ ILQN G +F  +YP+SI  +LG +S+L + G++ R + +I   F
Sbjct: 84  FGEPTIVSADAGLNRFILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSISLNF 143

Query: 135 LRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLVKVLMSVGPG-KELE 194
           L   +L+  + KD++      L +W  NS    Q EAK+ TF ++ K +MS+ PG +E E
Sbjct: 144 LSHARLRTILLKDVERHTLFVLDSWQQNSIFSAQDEAKKFTFNLMAKHIMSMDPGEEETE 203

Query: 195 TLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERKSRVGEKG------ 254
            LK+E+  F+K ++  P+ LPGT  +K+L++R  +LK + + +EERK  + E+       
Sbjct: 204 QLKKEYVTFMKGVVSAPLNLPGTAYHKALQSRATILKFIERKMEERKLDIKEEDQEEEEV 263

Query: 255 APRDAVDVLLQDHSENQ--------------GLPLDFISSHIIELMIPGEETVPTAMTLA 314
              D  ++   DH   Q               L  + I   I+ L+  G ET   A+ LA
Sbjct: 264 KTEDEAEMSKSDHVRKQRTDDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSVAIALA 323

Query: 315 VKFLGDCPRALAQLMEENMKLKKEKDGSGE-EYTWTDYMSLQFTQNVISETLRMANIVNG 374
           + FL  CP+A+ +L EE++++ + K   GE E  W DY  + FTQ VI+ETLR+ N+V  
Sbjct: 324 IFFLQACPKAVEELREEHLEIARAKKELGESELNWDDYKKMDFTQCVINETLRLGNVVRF 383

Query: 375 VWRKAQKDVKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEYGCNCKKYKQEN 434
           + RKA KDV+ K Y IP GW VL    +VH+D   Y  P+ F+PWRW+   N        
Sbjct: 384 LHRKALKDVRYKGYDIPSGWKVLPVISAVHLDNSRYDQPNLFNPWRWQQQNNGASSSGSG 443

Query: 435 LSATNNHNFTPFGGGQRLCPGVELTRLEISIFLHHLVTTYSQDEKE 459
             +T  +N+ PFGGG RLC G EL +LE+++F+HHLV  ++ +  E
Sbjct: 444 SFSTWGNNYMPFGGGPRLCAGSELAKLEMAVFIHHLVLKFNWELAE 489

BLAST of MELO3C007870 vs. TAIR 10
Match: AT5G05690.2 (Cytochrome P450 superfamily protein )

HSP 1 Score: 288.9 bits (738), Expect = 7.6e-78
Identity = 153/372 (41.13%), Postives = 232/372 (62.37%), Query Frame = 0

Query: 29  GIPKGNLGWPFFGETLQFISSGYSSRPVTFMDKRKSLYGNVFKTNILGTPIIVSTDSEVN 88
           G+P G+LG P  GET Q I +  +  P  F+D+R + YG+VF T++ G P I S D E N
Sbjct: 30  GLPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETN 89

Query: 89  KVILQNHGNIFTPAYPKSITELLGTYSILRMKGNVQRRLHTIIGAFLRSPQLKAQITKDI 148
           + +LQN G +F  +YP SI  LLG +S+L MKG++ +R+H++  +F  S  +K  +  DI
Sbjct: 90  RFVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDI 149

Query: 149 QNTVQLRLANWNNNSPLHFQTEAKQITFEVLVKVLMSVGPGKELETLKREFEEFIKALIC 208
              V+  L +W  +S +    EAK+ITFE+ VK LMS  PG+  E+L++E+   I+    
Sbjct: 150 DRLVRFNLDSW--SSRVLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFS 209

Query: 209 IPIKLPGTRLYKSLKAREKLLKIVGKIVEERKSRVGEKGAPRDAVDVLLQDHSENQGLPL 268
           +P+ L  T   K+++AR K+ + +  +V +R+    E+GA R   D+L    + + G   
Sbjct: 210 LPLPLFSTTYRKAIQARRKVAEALTVVVMKRREE-EEEGAERKK-DMLAALLAADDGFSD 269

Query: 269 DFISSHIIELMIPGEETVPTAMTLAVKFLGDCPRALAQLMEENMKLKKEKDGSGEEYTWT 328
           + I   ++ L++ G ET  T MTLAVKFL + P ALAQL EE+ K++  K  S     W+
Sbjct: 270 EEIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSDS-YSLEWS 329

Query: 329 DYMSLQFTQNVISETLRMANIVNGVWRKAQKDVKIKDYLIPQGWCVLASFISVHMDEKNY 388
           DY S+ FTQ V++ETLR+ANI+ GV+R+A  DV+IK Y IP+GW V +SF +VH+D  ++
Sbjct: 330 DYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHLDPNHF 389

Query: 389 ANPHEFDPWRWE 401
            +   F+PWRW+
Sbjct: 390 KDARTFNPWRWQ 396