MELO3C007734 (gene) Melon (DHL92) v4

BLAST of MELO3C007734 vs. NCBI nr
Match: XP_008440569.1 (PREDICTED: probable glucan 1,3-beta-glucosidase A [Cucumis melo])

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 530/530 (100.00%), Postives = 530/530 (100.00%), Query Frame = 0

Query: 1   MELVFTKWVFGFFLCCCLVFSKAYSVEGILGDDKVRGVNLGGWLVIEGWIKPSLFEGIPN 60
           MELVFTKWVFGFFLCCCLVFSKAYSVEGILGDDKVRGVNLGGWLVIEGWIKPSLFEGIPN
Sbjct: 1   MELVFTKWVFGFFLCCCLVFSKAYSVEGILGDDKVRGVNLGGWLVIEGWIKPSLFEGIPN 60

Query: 61  GDMLDGALVQLRSLTLQKFVSAENGGGTGVTVSRDVASSWETFRLWRVSASEFQFRTSLG 120
           GDMLDGALVQLRSLTLQKFVSAENGGGTGVTVSRDVASSWETFRLWRVSASEFQFRTSLG
Sbjct: 61  GDMLDGALVQLRSLTLQKFVSAENGGGTGVTVSRDVASSWETFRLWRVSASEFQFRTSLG 120

Query: 121 QFLTCDGLECSAAAQSPRNAATFVVERNSNRVHLKLKNGAYLQAMITNQLTADYPGKPGW 180
           QFLTCDGLECSAAAQSPRNAATFVVERNSNRVHLKLKNGAYLQAMITNQLTADYPGKPGW
Sbjct: 121 QFLTCDGLECSAAAQSPRNAATFVVERNSNRVHLKLKNGAYLQAMITNQLTADYPGKPGW 180

Query: 181 DDNAATFEMIVSNNLHGDYQLANGYGKDEATRVLQRHRNNFVTVDDFKFLYRHGINTVRI 240
           DDNAATFEMIVSNNLHGDYQLANGYGKDEATRVLQRHRNNFVTVDDFKFLYRHGINTVRI
Sbjct: 181 DDNAATFEMIVSNNLHGDYQLANGYGKDEATRVLQRHRNNFVTVDDFKFLYRHGINTVRI 240

Query: 241 PVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHSSSNDG 300
           PVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHSSSNDG
Sbjct: 241 PVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHSSSNDG 300

Query: 301 TIGWPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVPFDTVVSYYKQGYDI 360
           TIGWPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVPFDTVVSYYKQGYDI
Sbjct: 301 TIGWPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVPFDTVVSYYKQGYDI 360

Query: 361 VRKYSSTAYVIICQRIGKADPMELYQANIGSHNLVVDLHYYNLFDPFFDHLSPSENIEVI 420
           VRKYSSTAYVIICQRIGKADPMELYQANIGSHNLVVDLHYYNLFDPFFDHLSPSENIEVI
Sbjct: 361 VRKYSSTAYVIICQRIGKADPMELYQANIGSHNLVVDLHYYNLFDPFFDHLSPSENIEVI 420

Query: 421 YKNRQTQIQALNSANGPLVFVGEWSNEWNVTNASQADYQNFGRAQLEVYNAASFGWTYWT 480
           YKNRQTQIQALNSANGPLVFVGEWSNEWNVTNASQADYQNFGRAQLEVYNAASFGWTYWT
Sbjct: 421 YKNRQTQIQALNSANGPLVFVGEWSNEWNVTNASQADYQNFGRAQLEVYNAASFGWTYWT 480

Query: 481 LKNDRKHWDFEWNIKNNYLQFGDSPSRVIFNCYLLVALACGWFPHLLLLL 531
           LKNDRKHWDFEWNIKNNYLQFGDSPSRVIFNCYLLVALACGWFPHLLLLL
Sbjct: 481 LKNDRKHWDFEWNIKNNYLQFGDSPSRVIFNCYLLVALACGWFPHLLLLL 530

BLAST of MELO3C007734 vs. NCBI nr
Match: XP_004143452.1 (probable glucan 1,3-beta-glucosidase A isoform X1 [Cucumis sativus])

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 519/530 (97.92%), Postives = 524/530 (98.87%), Query Frame = 0

Query: 1   MELVFTKWVFGFFLCCCLVFSKAYSVEGILGDDKVRGVNLGGWLVIEGWIKPSLFEGIPN 60
           MELVFTKWV GFFLC  LVFSKAYSVEGILGD+KVRGVNLGGWLVIEGWIKPSLFEGIPN
Sbjct: 1   MELVFTKWVLGFFLCSYLVFSKAYSVEGILGDNKVRGVNLGGWLVIEGWIKPSLFEGIPN 60

Query: 61  GDMLDGALVQLRSLTLQKFVSAENGGGTGVTVSRDVASSWETFRLWRVSASEFQFRTSLG 120
           GDMLDGA+VQLRS+TLQKFVSAENGGGTGVTVSRDVASSWETFRLWRVSASEFQFRTSLG
Sbjct: 61  GDMLDGAVVQLRSVTLQKFVSAENGGGTGVTVSRDVASSWETFRLWRVSASEFQFRTSLG 120

Query: 121 QFLTCDGLECSAAAQSPRNAATFVVERNSNRVHLKLKNGAYLQAMITNQLTADYPGKPGW 180
           QFLTCDGLECSAAAQSPRN+ATFVVERN NRVHLKLKNGAYLQAMITNQLTADY GKPGW
Sbjct: 121 QFLTCDGLECSAAAQSPRNSATFVVERNGNRVHLKLKNGAYLQAMITNQLTADYLGKPGW 180

Query: 181 DDNAATFEMIVSNNLHGDYQLANGYGKDEATRVLQRHRNNFVTVDDFKFLYRHGINTVRI 240
           DDNAATFEMIVSNNLHGDYQLANGYGKDEATRVLQRHRNNFVTVDDFKFLYRHGINTVRI
Sbjct: 181 DDNAATFEMIVSNNLHGDYQLANGYGKDEATRVLQRHRNNFVTVDDFKFLYRHGINTVRI 240

Query: 241 PVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHSSSNDG 300
           PVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHSSSNDG
Sbjct: 241 PVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHSSSNDG 300

Query: 301 TIGWPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVPFDTVVSYYKQGYDI 360
           TIGWPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVPFDTVVSYYKQGYDI
Sbjct: 301 TIGWPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVPFDTVVSYYKQGYDI 360

Query: 361 VRKYSSTAYVIICQRIGKADPMELYQANIGSHNLVVDLHYYNLFDPFFDHLSPSENIEVI 420
           VRKYSSTAYVIICQRIGKADPMELYQAN+GSHNLVVDLHYYNLFDPFFDHLS SENIEVI
Sbjct: 361 VRKYSSTAYVIICQRIGKADPMELYQANVGSHNLVVDLHYYNLFDPFFDHLSASENIEVI 420

Query: 421 YKNRQTQIQALNSANGPLVFVGEWSNEWNVTNASQADYQNFGRAQLEVYNAASFGWTYWT 480
           YKNRQTQIQALNSANGPLVFVGEWSNEWNVTNASQADYQNFGRAQLEVYNAASFGWTYWT
Sbjct: 421 YKNRQTQIQALNSANGPLVFVGEWSNEWNVTNASQADYQNFGRAQLEVYNAASFGWTYWT 480

Query: 481 LKNDRKHWDFEWNIKNNYLQFGDSPSRVIFNCYLLVALACGWFPHLLLLL 531
           LKNDRKHWDFEWNIKNNYLQFGDSPSRVIFNCYLLVALACGWFPHLLLLL
Sbjct: 481 LKNDRKHWDFEWNIKNNYLQFGDSPSRVIFNCYLLVALACGWFPHLLLLL 530

BLAST of MELO3C007734 vs. NCBI nr
Match: XP_038880878.1 (probable glucan 1,3-beta-glucosidase A isoform X2 [Benincasa hispida])

HSP 1 Score: 1070.5 bits (2767), Expect = 4.9e-309
Identity = 503/530 (94.91%), Postives = 518/530 (97.74%), Query Frame = 0

Query: 1   MELVFTKWVFGFFLCCCLVFSKAYSVEGILGDDKVRGVNLGGWLVIEGWIKPSLFEGIPN 60
           MELVFTKWV GFFLCCCL+ S+AYSVEGILGDDK+RGVNLGGWLVIEGWIKPSLFEGIPN
Sbjct: 1   MELVFTKWVSGFFLCCCLISSQAYSVEGILGDDKIRGVNLGGWLVIEGWIKPSLFEGIPN 60

Query: 61  GDMLDGALVQLRSLTLQKFVSAENGGGTGVTVSRDVASSWETFRLWRVSASEFQFRTSLG 120
           GDMLDGA+VQLRS+TLQKFVSAENGGGTGVTV RD ASSWETFRLWRVSASEFQFRT LG
Sbjct: 61  GDMLDGAVVQLRSVTLQKFVSAENGGGTGVTVDRDAASSWETFRLWRVSASEFQFRTFLG 120

Query: 121 QFLTCDGLECSAAAQSPRNAATFVVERNSNRVHLKLKNGAYLQAMITNQLTADYPGKPGW 180
           QFLTCDGL CSAAAQSPRNAATFVVERN NRVHLKLK+G YLQAMITNQLTADYPGKPGW
Sbjct: 121 QFLTCDGLGCSAAAQSPRNAATFVVERNGNRVHLKLKSGVYLQAMITNQLTADYPGKPGW 180

Query: 181 DDNAATFEMIVSNNLHGDYQLANGYGKDEATRVLQRHRNNFVTVDDFKFLYRHGINTVRI 240
           DDNAATFEMIVSNNLHGDYQLANGYGKDEATRV QRHRNNF+T+DDFKFLYRHGINTVRI
Sbjct: 181 DDNAATFEMIVSNNLHGDYQLANGYGKDEATRVYQRHRNNFITIDDFKFLYRHGINTVRI 240

Query: 241 PVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHSSSNDG 300
           PVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHS+SNDG
Sbjct: 241 PVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHSASNDG 300

Query: 301 TIGWPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVPFDTVVSYYKQGYDI 360
           TIGWPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVPFDTVVSYYKQGY+I
Sbjct: 301 TIGWPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVPFDTVVSYYKQGYNI 360

Query: 361 VRKYSSTAYVIICQRIGKADPMELYQANIGSHNLVVDLHYYNLFDPFFDHLSPSENIEVI 420
           VRKYS TAYVIICQRIGKADPMELYQANIGSHNLVVDLHYYNLFDPFFDHLSPSENIE+I
Sbjct: 361 VRKYSPTAYVIICQRIGKADPMELYQANIGSHNLVVDLHYYNLFDPFFDHLSPSENIEII 420

Query: 421 YKNRQTQIQALNSANGPLVFVGEWSNEWNVTNASQADYQNFGRAQLEVYNAASFGWTYWT 480
           YKNRQTQIQALNSANGPL+F+GEW+NEWNVTNASQADYQNFGRAQLEVYNAASFGWTYWT
Sbjct: 421 YKNRQTQIQALNSANGPLIFIGEWANEWNVTNASQADYQNFGRAQLEVYNAASFGWTYWT 480

Query: 481 LKNDRKHWDFEWNIKNNYLQFGDSPSRVIFNCYLLVALACGWFPHLLLLL 531
           LKNDRKHWDFEWNIKNNYLQFGDSP+RVIFNC LLVALACGWFPHLL LL
Sbjct: 481 LKNDRKHWDFEWNIKNNYLQFGDSPNRVIFNCCLLVALACGWFPHLLFLL 530

BLAST of MELO3C007734 vs. NCBI nr
Match: XP_038880877.1 (probable glucan 1,3-beta-glucosidase A isoform X1 [Benincasa hispida])

HSP 1 Score: 1063.9 bits (2750), Expect = 4.4e-307
Identity = 503/536 (93.84%), Postives = 518/536 (96.64%), Query Frame = 0

Query: 1   MELVFTKWVFGFFLCCCLVFSKAYSVEGILGDDKVRGVNLGGWLVIEGWIKPSLFEGIPN 60
           MELVFTKWV GFFLCCCL+ S+AYSVEGILGDDK+RGVNLGGWLVIEGWIKPSLFEGIPN
Sbjct: 1   MELVFTKWVSGFFLCCCLISSQAYSVEGILGDDKIRGVNLGGWLVIEGWIKPSLFEGIPN 60

Query: 61  GDML------DGALVQLRSLTLQKFVSAENGGGTGVTVSRDVASSWETFRLWRVSASEFQ 120
           GDML      DGA+VQLRS+TLQKFVSAENGGGTGVTV RD ASSWETFRLWRVSASEFQ
Sbjct: 61  GDMLACGIIQDGAVVQLRSVTLQKFVSAENGGGTGVTVDRDAASSWETFRLWRVSASEFQ 120

Query: 121 FRTSLGQFLTCDGLECSAAAQSPRNAATFVVERNSNRVHLKLKNGAYLQAMITNQLTADY 180
           FRT LGQFLTCDGL CSAAAQSPRNAATFVVERN NRVHLKLK+G YLQAMITNQLTADY
Sbjct: 121 FRTFLGQFLTCDGLGCSAAAQSPRNAATFVVERNGNRVHLKLKSGVYLQAMITNQLTADY 180

Query: 181 PGKPGWDDNAATFEMIVSNNLHGDYQLANGYGKDEATRVLQRHRNNFVTVDDFKFLYRHG 240
           PGKPGWDDNAATFEMIVSNNLHGDYQLANGYGKDEATRV QRHRNNF+T+DDFKFLYRHG
Sbjct: 181 PGKPGWDDNAATFEMIVSNNLHGDYQLANGYGKDEATRVYQRHRNNFITIDDFKFLYRHG 240

Query: 241 INTVRIPVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEH 300
           INTVRIPVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEH
Sbjct: 241 INTVRIPVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEH 300

Query: 301 SSSNDGTIGWPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVPFDTVVSYY 360
           S+SNDGTIGWPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVPFDTVVSYY
Sbjct: 301 SASNDGTIGWPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVPFDTVVSYY 360

Query: 361 KQGYDIVRKYSSTAYVIICQRIGKADPMELYQANIGSHNLVVDLHYYNLFDPFFDHLSPS 420
           KQGY+IVRKYS TAYVIICQRIGKADPMELYQANIGSHNLVVDLHYYNLFDPFFDHLSPS
Sbjct: 361 KQGYNIVRKYSPTAYVIICQRIGKADPMELYQANIGSHNLVVDLHYYNLFDPFFDHLSPS 420

Query: 421 ENIEVIYKNRQTQIQALNSANGPLVFVGEWSNEWNVTNASQADYQNFGRAQLEVYNAASF 480
           ENIE+IYKNRQTQIQALNSANGPL+F+GEW+NEWNVTNASQADYQNFGRAQLEVYNAASF
Sbjct: 421 ENIEIIYKNRQTQIQALNSANGPLIFIGEWANEWNVTNASQADYQNFGRAQLEVYNAASF 480

Query: 481 GWTYWTLKNDRKHWDFEWNIKNNYLQFGDSPSRVIFNCYLLVALACGWFPHLLLLL 531
           GWTYWTLKNDRKHWDFEWNIKNNYLQFGDSP+RVIFNC LLVALACGWFPHLL LL
Sbjct: 481 GWTYWTLKNDRKHWDFEWNIKNNYLQFGDSPNRVIFNCCLLVALACGWFPHLLFLL 536

BLAST of MELO3C007734 vs. NCBI nr
Match: XP_022949769.1 (probable glucan 1,3-beta-glucosidase A isoform X1 [Cucurbita moschata])

HSP 1 Score: 1017.7 bits (2630), Expect = 3.7e-293
Identity = 480/530 (90.57%), Postives = 499/530 (94.15%), Query Frame = 0

Query: 1   MELVFTKWVFGFFLCCCLVFSKAYSVEGILGDDKVRGVNLGGWLVIEGWIKPSLFEGIPN 60
           MELVFTKWV  F LCCCL+ SKA+SVEGILGDDKVRGVNLGGWLVIEGWIKPSLFEGIPN
Sbjct: 1   MELVFTKWVSAFLLCCCLISSKAFSVEGILGDDKVRGVNLGGWLVIEGWIKPSLFEGIPN 60

Query: 61  GDMLDGALVQLRSLTLQKFVSAENGGGTGVTVSRDVASSWETFRLWRVSASEFQFRTSLG 120
           GDMLDG  VQLRS+TLQKFVSAENGGGTGV V+RDVASSWETFRLWRVSASEFQFRT LG
Sbjct: 61  GDMLDGDQVQLRSVTLQKFVSAENGGGTGVAVNRDVASSWETFRLWRVSASEFQFRTVLG 120

Query: 121 QFLTCDGLECSAAAQSPRNAATFVVERNSNRVHLKLKNGAYLQAMITNQLTADYPGKPGW 180
           QFLTCD L CSA AQSPRN+ TF VER  NRVHLKL+ GAYLQAMITNQLTADYPGKPGW
Sbjct: 121 QFLTCDALGCSATAQSPRNSETFFVERKDNRVHLKLQTGAYLQAMITNQLTADYPGKPGW 180

Query: 181 DDNAATFEMIVSNNLHGDYQLANGYGKDEATRVLQRHRNNFVTVDDFKFLYRHGINTVRI 240
           DDNAATFEMIVS NL+GDYQLANGYGKDEAT V QRHRNNFVT+DDFKFLYRHGINTVRI
Sbjct: 181 DDNAATFEMIVSGNLYGDYQLANGYGKDEATLVYQRHRNNFVTIDDFKFLYRHGINTVRI 240

Query: 241 PVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHSSSNDG 300
           PVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHS+SNDG
Sbjct: 241 PVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHSASNDG 300

Query: 301 TIGWPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVPFDTVVSYYKQGYDI 360
           T+ WPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVP DT+VSYYKQGY+I
Sbjct: 301 TLEWPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVPLDTLVSYYKQGYNI 360

Query: 361 VRKYSSTAYVIICQRIGKADPMELYQANIGSHNLVVDLHYYNLFDPFFDHLSPSENIEVI 420
           +RKYSS AYVIICQRIGKADPMELY+A+IGSHNLVVDLHYYNLFD FFDHLSPSENIE+I
Sbjct: 361 IRKYSSKAYVIICQRIGKADPMELYEADIGSHNLVVDLHYYNLFDRFFDHLSPSENIEII 420

Query: 421 YKNRQTQIQALNSANGPLVFVGEWSNEWNVTNASQADYQNFGRAQLEVYNAASFGWTYWT 480
           YKNRQTQIQALNSANGPLVFVGEW NEWNVTN SQADYQNFGRAQLEVYNAASFGWTYWT
Sbjct: 421 YKNRQTQIQALNSANGPLVFVGEWVNEWNVTNGSQADYQNFGRAQLEVYNAASFGWTYWT 480

Query: 481 LKNDRKHWDFEWNIKNNYLQFGDSPSRVIFNCYLLVALACGWFPHLLLLL 531
           LKND+KHWDFEWNIKNNYLQFGDSP R +FNC L VALAC WFPHLL +L
Sbjct: 481 LKNDKKHWDFEWNIKNNYLQFGDSPIRAVFNCGLWVALACAWFPHLLFML 530

BLAST of MELO3C007734 vs. ExPASy Swiss-Prot
Match: Q12626 (Glucan 1,3-beta-glucosidase OS=Pichia angusta OX=870730 PE=3 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 5.9e-36
Identity = 81/224 (36.16%), Postives = 121/224 (54.02%), Query Frame = 0

Query: 187 FEMIVSNNLHGDYQLANGYGKDEATRVLQRHRNNFVTVDDFKFLYRHGINTVRIPVGWWI 246
           FE + S+    +Y      GK+EA + LQ H + ++  +DFK +   G+N VRIP+G+W 
Sbjct: 69  FEAVSSDVPVDEYHYTEALGKEEAEKRLQEHWSTWIKEEDFKGMANAGLNFVRIPIGYW- 128

Query: 247 AFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHSSSNDGTIGWPN 306
           AF      P++ G  E LD A  W   Y L   +DLH APGSQNG ++S    G IGW N
Sbjct: 129 AFQLAEGDPYVQGQQEYLDKALEWCAKYGLKAWVDLHGAPGSQNGFDNSGKR-GEIGWQN 188

Query: 307 SPAYISKTLDVIDFLASRYG---KHPALLGIELLNEPSADLVPFDTVVSYYKQGYDIVRK 366
           +  Y+  T+ V+D L S+YG       ++GIELLNEP    + FD +V +Y +GY +VR 
Sbjct: 189 TTGYVDLTVQVLDQLTSKYGGSNYSDVIIGIELLNEPLGSYLDFDQLVDFYNKGYQLVRN 248

Query: 367 YSSTAYVIICQRI--GKADPMELYQANIGSHNLVVDLHYYNLFD 406
             +   +I    +     D +   + +     ++VD H+Y +FD
Sbjct: 249 NGNAPVIIHDAYLPDHTFDNVLNTEQDPNVWEVIVDHHHYQVFD 290

BLAST of MELO3C007734 vs. ExPASy Swiss-Prot
Match: A1CRV0 (Probable glucan 1,3-beta-glucosidase A OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1) OX=344612 GN=exgA PE=3 SV=2)

HSP 1 Score: 147.1 bits (370), Expect = 5.5e-34
Identity = 100/330 (30.30%), Postives = 154/330 (46.67%), Query Frame = 0

Query: 198 DYQLANGYGKDEATRVLQRHRNNFVTVDDFKFLYRHGINTVRIPVGWWIAFDPDPPAPFI 257
           ++ LA   GKD+A  +L +H ++F+T DDF  + + G+N VRIPVG+W    PD   P++
Sbjct: 72  EWTLAEVLGKDKARAILSQHWSSFITQDDFNQIAQAGMNHVRIPVGYWAVSAPD--EPYV 131

Query: 258 GGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHSSSNDGTIGWPNSPAYISKTLDV 317
            G LE LDNA SWA+A  L  +IDLH APGSQNG + +S   G I W      +++T+D 
Sbjct: 132 DGQLEFLDNAISWARAAGLKVMIDLHGAPGSQNGFD-NSGRKGPIAWQQGDT-VARTVDA 191

Query: 318 IDFLASRY-GKHPALLGIELLNEPS-ADLVPFDTVVSYYKQGYDIVRKYSSTAYVIICQR 377
              LA RY  +   +  IE +NEP+    V    +  YY Q  ++V   +  A V +   
Sbjct: 192 FKALAERYLPESDVVTAIEAVNEPNIPGGVNEGQLKEYYNQVLEVVHSINPDAGVFLSDG 251

Query: 378 IGKADPMELYQANIGSHNLVVDLHYYNLFD----------------PFFDHLSPSENIEV 437
                    Y       N+V+D H+Y++FD                 F + +  S+   V
Sbjct: 252 FLATASWNGY---ANGENVVMDTHHYHMFDNTLISLDINAHVRAACEFGNQIKGSDKPVV 311

Query: 438 IYKNRQTQIQALNSANG---PLVFVGEWSN--------------EWNVTNASQADYQNFG 492
           + +            NG   P  + G+W+N                 ++   ++D + F 
Sbjct: 312 VGEWTGALTDCTKHLNGKDIPTRYEGQWANSPRYGDCGNKRQGSSSGLSEQERSDTRRFI 371

HSP 2 Score: 45.1 bits (105), Expect = 3.0e-03
Identity = 18/22 (81.82%), Postives = 20/22 (90.91%), Query Frame = 0

Query: 35 VRGVNLGGWLVIEGWIKPSLFE 57
          VRGVNLGGWLV+E WI PS+FE
Sbjct: 42 VRGVNLGGWLVLEPWITPSIFE 63

BLAST of MELO3C007734 vs. ExPASy Swiss-Prot
Match: B0XN12 (Probable glucan 1,3-beta-glucosidase A OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=exgA PE=3 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 4.0e-32
Identity = 103/335 (30.75%), Postives = 156/335 (46.57%), Query Frame = 0

Query: 198 DYQLANGYGKDEATRVLQRHRNNFVTVDDFKFLYRHGINTVRIPVGWWIAFDPDPPAPFI 257
           ++ L    G+D+A  VL +H + F+T DDF+ + + G+N VRIP+G+W A    P  P++
Sbjct: 72  EWTLTATLGQDQAKAVLSQHWSTFITQDDFQQIAQAGMNHVRIPIGYW-AVSSLPDEPYV 131

Query: 258 GGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHSSSNDGTIGWPNSPAYISKTLDV 317
            G LE LDNA SWA+   L  +IDLH APGSQNG + +S   G I W      +S+T+D 
Sbjct: 132 DGQLEYLDNAISWAREAGLKVVIDLHGAPGSQNGFD-NSGRKGPIAWQQGDT-VSQTVDA 191

Query: 318 IDFLASRY-GKHPALLGIELLNEPS-ADLVPFDTVVSYYKQGYDIVRKYSSTAYVIICQR 377
              LA RY  +   +  IE LNEP+    V    +  YY Q  D+VR+      V +   
Sbjct: 192 FRALAERYLPQSDVVTAIEALNEPNIPGGVSEAGLRDYYNQIADVVRQIDPDTSVFLSDG 251

Query: 378 IGKADPMELYQANIGSHNLVVDLHYYNLFDPFFDHLSPSENIEVIYKNRQTQIQALNSAN 437
               +    ++      ++V+D H+Y +FD +   L    +I+   K+     + +  ++
Sbjct: 252 FLSTESWNGFKT---GEDVVMDTHHYEMFDNYLISL----DIDGHVKSACDFGKQIEGSD 311

Query: 438 GPLVFVGEWS----------NEWNVTNASQADYQN------------------------- 492
            P+V VGEWS          N   V+   Q +Y N                         
Sbjct: 312 KPVV-VGEWSGAVTDCTKHLNGKGVSTRYQGEYANNVKYGDCANTTQGSVADLSDQERTD 371

BLAST of MELO3C007734 vs. ExPASy Swiss-Prot
Match: Q4WK60 (Probable glucan 1,3-beta-glucosidase A OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=exgA PE=3 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 5.2e-32
Identity = 103/335 (30.75%), Postives = 156/335 (46.57%), Query Frame = 0

Query: 198 DYQLANGYGKDEATRVLQRHRNNFVTVDDFKFLYRHGINTVRIPVGWWIAFDPDPPAPFI 257
           ++ L    G+D+A  VL +H + F+T DDF+ + + G+N VRIP+G+W A    P  P++
Sbjct: 72  EWTLTATLGQDQAKAVLSQHWSTFITQDDFQQIAQAGMNHVRIPIGYW-AVSSLPDEPYV 131

Query: 258 GGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHSSSNDGTIGWPNSPAYISKTLDV 317
            G LE LDNA SWA+   L  +IDLH APGSQNG + +S   G I W      +S+T+D 
Sbjct: 132 DGQLEYLDNAISWAREAGLKVVIDLHGAPGSQNGFD-NSGRKGPIAWQQGDT-VSQTVDA 191

Query: 318 IDFLASRY-GKHPALLGIELLNEPS-ADLVPFDTVVSYYKQGYDIVRKYSSTAYVIICQR 377
              LA RY  +   +  IE LNEP+    V    +  YY Q  D+VR+      V +   
Sbjct: 192 FRALAERYLPQSDVVTAIEALNEPNIPGGVSEAGLRDYYNQIADVVRQIDPGTSVFLSDG 251

Query: 378 IGKADPMELYQANIGSHNLVVDLHYYNLFDPFFDHLSPSENIEVIYKNRQTQIQALNSAN 437
               +    ++      ++V+D H+Y +FD +   L    +I+   K+     + +  ++
Sbjct: 252 FLSTESWNGFKT---GEDVVMDTHHYEMFDNYLISL----DIDGHVKSACDFGKQIEGSD 311

Query: 438 GPLVFVGEWS----------NEWNVTNASQADYQN------------------------- 492
            P+V VGEWS          N   V+   Q +Y N                         
Sbjct: 312 KPVV-VGEWSGAVTDCTKHLNGKGVSTRYQGEYANNVKYGDCANTTQGSVADLSDQERTD 371

BLAST of MELO3C007734 vs. ExPASy Swiss-Prot
Match: Q9URU6 (Glucan 1,3-beta-glucosidase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=exg1 PE=2 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 3.4e-31
Identity = 93/314 (29.62%), Postives = 145/314 (46.18%), Query Frame = 0

Query: 191 VSNNLHGDYQLANGYGKDEATRVLQRHRNNFVTVDDFKFLYRHGINTVRIPVGWWIAFDP 250
           +SN    ++      G DEA   L  H ++F T  DF  +   G+N +RIP+G+W AF+ 
Sbjct: 65  MSNPPTDEWGFCEVLGADEAASQLAAHYSSFYTESDFATIASWGVNVLRIPIGYW-AFNV 124

Query: 251 DPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHSSSNDGTIGWPNSPAY 310
               P++ G    LD A +WA+ Y L   IDLH  PGSQNG E+S    G+IGW  +   
Sbjct: 125 VDGEPYVQGQEYWLDQALTWAEQYGLKVWIDLHGVPGSQNGFENSGKT-GSIGWQQNDT- 184

Query: 311 ISKTLDVIDFLASRYGKH---PALLGIELLNEPSADLVPFDTVVSYYKQGYDIVRKYSST 370
           +++TLD+I ++A++Y +      ++GIE +NEP    +  D +  Y    Y+IV   SS+
Sbjct: 185 VTRTLDIITYVANKYTQSQYASVVIGIETVNEPLGYGLDMDQLKQYDLDAYNIVNPLSSS 244

Query: 371 AYVIICQRIGKADPMELYQANIGSHNLVVDLHYYNLFD------PFFDHL---------- 430
              II          +    +  S+NLV+D+H Y L++         DHL          
Sbjct: 245 VATIIHDAYVDLSIWDYGVVSPSSYNLVMDVHRYQLYESDECSKTLDDHLSDVCSIGDSI 304

Query: 431 --SPSENIEVIYKNRQTQIQALNSANGPLVFVGEWSNEWNV-TNASQADYQNFGRAQLEV 483
             SP   +   +                  F+G  S + +  T+  +   + F   QL+ 
Sbjct: 305 ASSPYITVTGEWSGTLADCTIFEEGVDSSTFIGPNSGDISTWTDEYKGAVRLFIETQLDQ 364

HSP 2 Score: 45.4 bits (106), Expect = 2.3e-03
Identity = 29/59 (49.15%), Postives = 34/59 (57.63%), Query Frame = 0

Query: 3  LVFTKWVFGFFLCCCLV---FSKAYSVEGILGD---DKVRGVNLGGWLVIEGWIKPSLF 56
          L FT  VF FFL   L+   FS        + D   +KVRGVN+GGWLV+E WI P LF
Sbjct: 2  LSFTS-VFSFFLHALLLKTAFSYVIKRNNPVFDYTSEKVRGVNIGGWLVLENWITPQLF 59

BLAST of MELO3C007734 vs. ExPASy TrEMBL
Match: A0A1S3B1G2 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103484948 PE=3 SV=1)

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 530/530 (100.00%), Postives = 530/530 (100.00%), Query Frame = 0

Query: 1   MELVFTKWVFGFFLCCCLVFSKAYSVEGILGDDKVRGVNLGGWLVIEGWIKPSLFEGIPN 60
           MELVFTKWVFGFFLCCCLVFSKAYSVEGILGDDKVRGVNLGGWLVIEGWIKPSLFEGIPN
Sbjct: 1   MELVFTKWVFGFFLCCCLVFSKAYSVEGILGDDKVRGVNLGGWLVIEGWIKPSLFEGIPN 60

Query: 61  GDMLDGALVQLRSLTLQKFVSAENGGGTGVTVSRDVASSWETFRLWRVSASEFQFRTSLG 120
           GDMLDGALVQLRSLTLQKFVSAENGGGTGVTVSRDVASSWETFRLWRVSASEFQFRTSLG
Sbjct: 61  GDMLDGALVQLRSLTLQKFVSAENGGGTGVTVSRDVASSWETFRLWRVSASEFQFRTSLG 120

Query: 121 QFLTCDGLECSAAAQSPRNAATFVVERNSNRVHLKLKNGAYLQAMITNQLTADYPGKPGW 180
           QFLTCDGLECSAAAQSPRNAATFVVERNSNRVHLKLKNGAYLQAMITNQLTADYPGKPGW
Sbjct: 121 QFLTCDGLECSAAAQSPRNAATFVVERNSNRVHLKLKNGAYLQAMITNQLTADYPGKPGW 180

Query: 181 DDNAATFEMIVSNNLHGDYQLANGYGKDEATRVLQRHRNNFVTVDDFKFLYRHGINTVRI 240
           DDNAATFEMIVSNNLHGDYQLANGYGKDEATRVLQRHRNNFVTVDDFKFLYRHGINTVRI
Sbjct: 181 DDNAATFEMIVSNNLHGDYQLANGYGKDEATRVLQRHRNNFVTVDDFKFLYRHGINTVRI 240

Query: 241 PVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHSSSNDG 300
           PVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHSSSNDG
Sbjct: 241 PVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHSSSNDG 300

Query: 301 TIGWPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVPFDTVVSYYKQGYDI 360
           TIGWPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVPFDTVVSYYKQGYDI
Sbjct: 301 TIGWPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVPFDTVVSYYKQGYDI 360

Query: 361 VRKYSSTAYVIICQRIGKADPMELYQANIGSHNLVVDLHYYNLFDPFFDHLSPSENIEVI 420
           VRKYSSTAYVIICQRIGKADPMELYQANIGSHNLVVDLHYYNLFDPFFDHLSPSENIEVI
Sbjct: 361 VRKYSSTAYVIICQRIGKADPMELYQANIGSHNLVVDLHYYNLFDPFFDHLSPSENIEVI 420

Query: 421 YKNRQTQIQALNSANGPLVFVGEWSNEWNVTNASQADYQNFGRAQLEVYNAASFGWTYWT 480
           YKNRQTQIQALNSANGPLVFVGEWSNEWNVTNASQADYQNFGRAQLEVYNAASFGWTYWT
Sbjct: 421 YKNRQTQIQALNSANGPLVFVGEWSNEWNVTNASQADYQNFGRAQLEVYNAASFGWTYWT 480

Query: 481 LKNDRKHWDFEWNIKNNYLQFGDSPSRVIFNCYLLVALACGWFPHLLLLL 531
           LKNDRKHWDFEWNIKNNYLQFGDSPSRVIFNCYLLVALACGWFPHLLLLL
Sbjct: 481 LKNDRKHWDFEWNIKNNYLQFGDSPSRVIFNCYLLVALACGWFPHLLLLL 530

BLAST of MELO3C007734 vs. ExPASy TrEMBL
Match: A0A0A0KLU4 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G499210 PE=3 SV=1)

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 519/530 (97.92%), Postives = 524/530 (98.87%), Query Frame = 0

Query: 1   MELVFTKWVFGFFLCCCLVFSKAYSVEGILGDDKVRGVNLGGWLVIEGWIKPSLFEGIPN 60
           MELVFTKWV GFFLC  LVFSKAYSVEGILGD+KVRGVNLGGWLVIEGWIKPSLFEGIPN
Sbjct: 1   MELVFTKWVLGFFLCSYLVFSKAYSVEGILGDNKVRGVNLGGWLVIEGWIKPSLFEGIPN 60

Query: 61  GDMLDGALVQLRSLTLQKFVSAENGGGTGVTVSRDVASSWETFRLWRVSASEFQFRTSLG 120
           GDMLDGA+VQLRS+TLQKFVSAENGGGTGVTVSRDVASSWETFRLWRVSASEFQFRTSLG
Sbjct: 61  GDMLDGAVVQLRSVTLQKFVSAENGGGTGVTVSRDVASSWETFRLWRVSASEFQFRTSLG 120

Query: 121 QFLTCDGLECSAAAQSPRNAATFVVERNSNRVHLKLKNGAYLQAMITNQLTADYPGKPGW 180
           QFLTCDGLECSAAAQSPRN+ATFVVERN NRVHLKLKNGAYLQAMITNQLTADY GKPGW
Sbjct: 121 QFLTCDGLECSAAAQSPRNSATFVVERNGNRVHLKLKNGAYLQAMITNQLTADYLGKPGW 180

Query: 181 DDNAATFEMIVSNNLHGDYQLANGYGKDEATRVLQRHRNNFVTVDDFKFLYRHGINTVRI 240
           DDNAATFEMIVSNNLHGDYQLANGYGKDEATRVLQRHRNNFVTVDDFKFLYRHGINTVRI
Sbjct: 181 DDNAATFEMIVSNNLHGDYQLANGYGKDEATRVLQRHRNNFVTVDDFKFLYRHGINTVRI 240

Query: 241 PVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHSSSNDG 300
           PVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHSSSNDG
Sbjct: 241 PVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHSSSNDG 300

Query: 301 TIGWPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVPFDTVVSYYKQGYDI 360
           TIGWPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVPFDTVVSYYKQGYDI
Sbjct: 301 TIGWPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVPFDTVVSYYKQGYDI 360

Query: 361 VRKYSSTAYVIICQRIGKADPMELYQANIGSHNLVVDLHYYNLFDPFFDHLSPSENIEVI 420
           VRKYSSTAYVIICQRIGKADPMELYQAN+GSHNLVVDLHYYNLFDPFFDHLS SENIEVI
Sbjct: 361 VRKYSSTAYVIICQRIGKADPMELYQANVGSHNLVVDLHYYNLFDPFFDHLSASENIEVI 420

Query: 421 YKNRQTQIQALNSANGPLVFVGEWSNEWNVTNASQADYQNFGRAQLEVYNAASFGWTYWT 480
           YKNRQTQIQALNSANGPLVFVGEWSNEWNVTNASQADYQNFGRAQLEVYNAASFGWTYWT
Sbjct: 421 YKNRQTQIQALNSANGPLVFVGEWSNEWNVTNASQADYQNFGRAQLEVYNAASFGWTYWT 480

Query: 481 LKNDRKHWDFEWNIKNNYLQFGDSPSRVIFNCYLLVALACGWFPHLLLLL 531
           LKNDRKHWDFEWNIKNNYLQFGDSPSRVIFNCYLLVALACGWFPHLLLLL
Sbjct: 481 LKNDRKHWDFEWNIKNNYLQFGDSPSRVIFNCYLLVALACGWFPHLLLLL 530

BLAST of MELO3C007734 vs. ExPASy TrEMBL
Match: A0A6J1GDW7 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111453058 PE=3 SV=1)

HSP 1 Score: 1017.7 bits (2630), Expect = 1.8e-293
Identity = 480/530 (90.57%), Postives = 499/530 (94.15%), Query Frame = 0

Query: 1   MELVFTKWVFGFFLCCCLVFSKAYSVEGILGDDKVRGVNLGGWLVIEGWIKPSLFEGIPN 60
           MELVFTKWV  F LCCCL+ SKA+SVEGILGDDKVRGVNLGGWLVIEGWIKPSLFEGIPN
Sbjct: 1   MELVFTKWVSAFLLCCCLISSKAFSVEGILGDDKVRGVNLGGWLVIEGWIKPSLFEGIPN 60

Query: 61  GDMLDGALVQLRSLTLQKFVSAENGGGTGVTVSRDVASSWETFRLWRVSASEFQFRTSLG 120
           GDMLDG  VQLRS+TLQKFVSAENGGGTGV V+RDVASSWETFRLWRVSASEFQFRT LG
Sbjct: 61  GDMLDGDQVQLRSVTLQKFVSAENGGGTGVAVNRDVASSWETFRLWRVSASEFQFRTVLG 120

Query: 121 QFLTCDGLECSAAAQSPRNAATFVVERNSNRVHLKLKNGAYLQAMITNQLTADYPGKPGW 180
           QFLTCD L CSA AQSPRN+ TF VER  NRVHLKL+ GAYLQAMITNQLTADYPGKPGW
Sbjct: 121 QFLTCDALGCSATAQSPRNSETFFVERKDNRVHLKLQTGAYLQAMITNQLTADYPGKPGW 180

Query: 181 DDNAATFEMIVSNNLHGDYQLANGYGKDEATRVLQRHRNNFVTVDDFKFLYRHGINTVRI 240
           DDNAATFEMIVS NL+GDYQLANGYGKDEAT V QRHRNNFVT+DDFKFLYRHGINTVRI
Sbjct: 181 DDNAATFEMIVSGNLYGDYQLANGYGKDEATLVYQRHRNNFVTIDDFKFLYRHGINTVRI 240

Query: 241 PVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHSSSNDG 300
           PVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHS+SNDG
Sbjct: 241 PVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHSASNDG 300

Query: 301 TIGWPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVPFDTVVSYYKQGYDI 360
           T+ WPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVP DT+VSYYKQGY+I
Sbjct: 301 TLEWPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVPLDTLVSYYKQGYNI 360

Query: 361 VRKYSSTAYVIICQRIGKADPMELYQANIGSHNLVVDLHYYNLFDPFFDHLSPSENIEVI 420
           +RKYSS AYVIICQRIGKADPMELY+A+IGSHNLVVDLHYYNLFD FFDHLSPSENIE+I
Sbjct: 361 IRKYSSKAYVIICQRIGKADPMELYEADIGSHNLVVDLHYYNLFDRFFDHLSPSENIEII 420

Query: 421 YKNRQTQIQALNSANGPLVFVGEWSNEWNVTNASQADYQNFGRAQLEVYNAASFGWTYWT 480
           YKNRQTQIQALNSANGPLVFVGEW NEWNVTN SQADYQNFGRAQLEVYNAASFGWTYWT
Sbjct: 421 YKNRQTQIQALNSANGPLVFVGEWVNEWNVTNGSQADYQNFGRAQLEVYNAASFGWTYWT 480

Query: 481 LKNDRKHWDFEWNIKNNYLQFGDSPSRVIFNCYLLVALACGWFPHLLLLL 531
           LKND+KHWDFEWNIKNNYLQFGDSP R +FNC L VALAC WFPHLL +L
Sbjct: 481 LKNDKKHWDFEWNIKNNYLQFGDSPIRAVFNCGLWVALACAWFPHLLFML 530

BLAST of MELO3C007734 vs. ExPASy TrEMBL
Match: A0A6J1IU40 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111478434 PE=3 SV=1)

HSP 1 Score: 1008.8 bits (2607), Expect = 8.2e-291
Identity = 476/530 (89.81%), Postives = 496/530 (93.58%), Query Frame = 0

Query: 1   MELVFTKWVFGFFLCCCLVFSKAYSVEGILGDDKVRGVNLGGWLVIEGWIKPSLFEGIPN 60
           MELVFTKWV  FFLCCCL+ SKA+SVEGILGD+KVRGVNLGGWLVIEGWIKPSLFEGIPN
Sbjct: 1   MELVFTKWVSAFFLCCCLISSKAFSVEGILGDNKVRGVNLGGWLVIEGWIKPSLFEGIPN 60

Query: 61  GDMLDGALVQLRSLTLQKFVSAENGGGTGVTVSRDVASSWETFRLWRVSASEFQFRTSLG 120
           GDMLDG  VQL S+TL+KFVSAENGGGTGV V RDVASSWETFRLWRVSASEFQFRT LG
Sbjct: 61  GDMLDGNQVQLWSVTLKKFVSAENGGGTGVAVDRDVASSWETFRLWRVSASEFQFRTVLG 120

Query: 121 QFLTCDGLECSAAAQSPRNAATFVVERNSNRVHLKLKNGAYLQAMITNQLTADYPGKPGW 180
           QFL CD L CSA AQSPRN+ TF VER  NRVHLKL+ GAYLQAMITNQLTADYPGKPGW
Sbjct: 121 QFLKCDALGCSATAQSPRNSETFFVERKDNRVHLKLRTGAYLQAMITNQLTADYPGKPGW 180

Query: 181 DDNAATFEMIVSNNLHGDYQLANGYGKDEATRVLQRHRNNFVTVDDFKFLYRHGINTVRI 240
           DDNAATFEMIVS NLHGDYQLANGYGKDEATRV QRHRNNFVT+DDFKFLYRHGINTVRI
Sbjct: 181 DDNAATFEMIVSGNLHGDYQLANGYGKDEATRVYQRHRNNFVTIDDFKFLYRHGINTVRI 240

Query: 241 PVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHSSSNDG 300
           PVGWWIAFDPDPPAPFIGGSLEALDNAFSWA AYNLMCIIDLHAAPGSQNGMEHS+SNDG
Sbjct: 241 PVGWWIAFDPDPPAPFIGGSLEALDNAFSWALAYNLMCIIDLHAAPGSQNGMEHSASNDG 300

Query: 301 TIGWPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVPFDTVVSYYKQGYDI 360
           T+ WPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVP DT+VSYYKQGY+I
Sbjct: 301 TLEWPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVPLDTLVSYYKQGYNI 360

Query: 361 VRKYSSTAYVIICQRIGKADPMELYQANIGSHNLVVDLHYYNLFDPFFDHLSPSENIEVI 420
           VRKYSS AYVIICQRIGKADPMELY+A+IGSHNLVVDLH YNLFDPFFDHLSPSENIE+I
Sbjct: 361 VRKYSSKAYVIICQRIGKADPMELYEADIGSHNLVVDLHCYNLFDPFFDHLSPSENIEII 420

Query: 421 YKNRQTQIQALNSANGPLVFVGEWSNEWNVTNASQADYQNFGRAQLEVYNAASFGWTYWT 480
           YKNRQTQ+QALNSANGPLVFVGEW NEW+VTN SQADYQNFGR QLEVYNAASFGWTYWT
Sbjct: 421 YKNRQTQLQALNSANGPLVFVGEWVNEWDVTNGSQADYQNFGRVQLEVYNAASFGWTYWT 480

Query: 481 LKNDRKHWDFEWNIKNNYLQFGDSPSRVIFNCYLLVALACGWFPHLLLLL 531
           LKNDRKHWDFEWNIKNNYLQFGDSP R +F+C L VALAC WFPHLL +L
Sbjct: 481 LKNDRKHWDFEWNIKNNYLQFGDSPIRAVFDCGLWVALACAWFPHLLFML 530

BLAST of MELO3C007734 vs. ExPASy TrEMBL
Match: A0A6J1BTP0 (Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111005448 PE=3 SV=1)

HSP 1 Score: 1002.7 bits (2591), Expect = 5.9e-289
Identity = 473/530 (89.25%), Postives = 492/530 (92.83%), Query Frame = 0

Query: 1   MELVFTKWVFGFFLCCCLVFSKAYSVEGILGDDKVRGVNLGGWLVIEGWIKPSLFEGIPN 60
           MEL+ TKWV  F LCCCL+ SKAYSVEGI GDDKVRGVNLGGWLVIEGWIKPSLFEGIPN
Sbjct: 1   MELIVTKWVSAFLLCCCLISSKAYSVEGIHGDDKVRGVNLGGWLVIEGWIKPSLFEGIPN 60

Query: 61  GDMLDGALVQLRSLTLQKFVSAENGGGTGVTVSRDVASSWETFRLWRVSASEFQFRTSLG 120
           GDMLDG  VQ RS+TLQKFVSA+NGGG+GV+VSRDVASSWETFRLWRVSASEFQFRT  G
Sbjct: 61  GDMLDGTEVQFRSVTLQKFVSADNGGGSGVSVSRDVASSWETFRLWRVSASEFQFRTFQG 120

Query: 121 QFLTCDGLECSAAAQSPRNAATFVVERNSNRVHLKLKNGAYLQAMITNQLTADYPGKPGW 180
           QFLTCDG  CSA  QSPRN+ TF VER  NRVHLKLKNGAYLQAMITNQLTADYPGKPGW
Sbjct: 121 QFLTCDGQGCSATVQSPRNSETFFVERKGNRVHLKLKNGAYLQAMITNQLTADYPGKPGW 180

Query: 181 DDNAATFEMIVSNNLHGDYQLANGYGKDEATRVLQRHRNNFVTVDDFKFLYRHGINTVRI 240
           DDNAATFEMI+SNNLHGDYQLANGYGKDEA+RVLQRHRNNFVT+DDFKFLYRHGINTVRI
Sbjct: 181 DDNAATFEMILSNNLHGDYQLANGYGKDEASRVLQRHRNNFVTIDDFKFLYRHGINTVRI 240

Query: 241 PVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNLMCIIDLHAAPGSQNGMEHSSSNDG 300
           PVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYN+MCIIDLHAAPGSQNGMEHSSSNDG
Sbjct: 241 PVGWWIAFDPDPPAPFIGGSLEALDNAFSWAQAYNIMCIIDLHAAPGSQNGMEHSSSNDG 300

Query: 301 TIGWPNSPAYISKTLDVIDFLASRYGKHPALLGIELLNEPSADLVPFDTVVSYYKQGYDI 360
           T GW NSPAYISKTLDVIDFLASRYGKHP+LLGIELLNEPSA LVP DT+VSYYKQGYDI
Sbjct: 301 TTGWANSPAYISKTLDVIDFLASRYGKHPSLLGIELLNEPSAALVPLDTLVSYYKQGYDI 360

Query: 361 VRKYSSTAYVIICQRIGKADPMELYQANIGSHNLVVDLHYYNLFDPFFDHLSPSENIEVI 420
           VRKYSSTAYVIICQRIG ADPMELYQANIGSHNLVVDLHYYNLFDPFFDHLSPS+NIEVI
Sbjct: 361 VRKYSSTAYVIICQRIGNADPMELYQANIGSHNLVVDLHYYNLFDPFFDHLSPSDNIEVI 420

Query: 421 YKNRQTQIQALNSANGPLVFVGEWSNEWNVTNASQADYQNFGRAQLEVYNAASFGWTYWT 480
           YK+RQTQ+QALN+ANGPLVFVGEW NEWNV+N SQADYQNFGRAQLEVYNAASFGWTYWT
Sbjct: 421 YKSRQTQLQALNAANGPLVFVGEWVNEWNVSNGSQADYQNFGRAQLEVYNAASFGWTYWT 480

Query: 481 LKNDRKHWDFEWNIKNNYLQFGDSPSRVIFNCYLLVALACGWFPHLLLLL 531
           LKNDRKHWDFEWNIKNNYLQ G SPS +IFN   L  LAC WF HL  LL
Sbjct: 481 LKNDRKHWDFEWNIKNNYLQLGGSPSMIIFNSCFLFGLACAWFSHLQFLL 530