MELO3C007283.jh1 (gene) Melon (Harukei-3) v1.41

BLAST of MELO3C007283.jh1 vs. NCBI nr
Match: XP_008439854.1 (PREDICTED: probable ubiquitin-conjugating enzyme E2 24 [Cucumis melo] >XP_008439855.1 PREDICTED: probable ubiquitin-conjugating enzyme E2 24 [Cucumis melo] >XP_008439856.1 PREDICTED: probable ubiquitin-conjugating enzyme E2 24 [Cucumis melo] >XP_008439858.1 PREDICTED: probable ubiquitin-conjugating enzyme E2 24 [Cucumis melo] >KAA0052687.1 putative ubiquitin-conjugating enzyme E2 24 [Cucumis melo var. makuwa] >TYK13137.1 putative ubiquitin-conjugating enzyme E2 24 [Cucumis melo var. makuwa])

HSP 1 Score: 1856 bits (4807), Expect = 0.0
Identity = 909/911 (99.78%), Postives = 911/911 (100.00%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60
           MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD
Sbjct: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60

Query: 61  AVCFVNDPYGQMGKVVGIEMLVDLEKNSGPLIKNVNSKDLLKIRSISAGDVVVNGPWIGR 120
           AVCFVNDPYGQMGKVVGIEMLVDLEKNSGPLIKNVNSKDLLKIRSISAGDVVVNGPWIGR
Sbjct: 61  AVCFVNDPYGQMGKVVGIEMLVDLEKNSGPLIKNVNSKDLLKIRSISAGDVVVNGPWIGR 120

Query: 121 VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180
           VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW
Sbjct: 121 VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180

Query: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASILVGCSSSLTAPPRMQHAKELTLLSCFSHANW 240
           LSGWKDDRCEGTVCSVEAGLVYVDWLASIL+GCSSSLTAPPRMQHAKELTLLSCFSHANW
Sbjct: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASILMGCSSSLTAPPRMQHAKELTLLSCFSHANW 240

Query: 241 NLGDWCIFPGDEKNNYFGEKFNSTHDLMNGGYRGTGFPEICIIVKKKTKVDVLWQDGSRS 300
           NLGDWCIFPGDEKNNYFGEKFNSTHDLMNGGYRGTGFPEICIIVKKKTKVDVLWQDGSRS
Sbjct: 241 NLGDWCIFPGDEKNNYFGEKFNSTHDLMNGGYRGTGFPEICIIVKKKTKVDVLWQDGSRS 300

Query: 301 TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGIVQAVDAKERTVKVQLR 360
           TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWG+VQAVDAKERTVKVQLR
Sbjct: 301 TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGVVQAVDAKERTVKVQLR 360

Query: 361 TNDLSEGSHLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLAMDKFASQSDENNIISEIS 420
           TNDLSEGSHLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLAMDKFASQSDENNIISEIS
Sbjct: 361 TNDLSEGSHLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLAMDKFASQSDENNIISEIS 420

Query: 421 MNESAQNEGNHIINNNNMNMDNFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK 480
           MNESAQNEGNHIINNNNMNMDNFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK
Sbjct: 421 MNESAQNEGNHIINNNNMNMDNFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK 480

Query: 481 YEVSSIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE 540
           YEVSSIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE
Sbjct: 481 YEVSSIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE 540

Query: 541 FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEIDLLCEKEVLQTCSQSTEMSQIAKLHN 600
           FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEIDLLCEKEVLQTCSQSTEMSQIAKLHN
Sbjct: 541 FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEIDLLCEKEVLQTCSQSTEMSQIAKLHN 600

Query: 601 FGETSLDIEEIHKNEDLTFSTSSKNYDLLTQFDMASDCPDHHFLSSGKGSMLSQASRKWL 660
           FGETSLDIEEIHKNEDLTFSTSSKNYDLLTQFDMASDCPDHHFLSSGKGSMLSQASRKWL
Sbjct: 601 FGETSLDIEEIHKNEDLTFSTSSKNYDLLTQFDMASDCPDHHFLSSGKGSMLSQASRKWL 660

Query: 661 KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI 720
           KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI
Sbjct: 661 KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI 720

Query: 721 PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780
           PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE
Sbjct: 721 PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780

Query: 781 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFETLVEEHFRRRY 840
           KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFETLVEEHFRRRY
Sbjct: 781 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFETLVEEHFRRRY 840

Query: 841 QHILAACRAYMEGTPVGSDYQRGTTKPENPNQSSTGFKIMLAKIFPRLIKTFSSKGFDCS 900
           QHILAACRAYMEGTPVGSDYQRGTTKPENPNQSSTGFKIMLAKIFPRLIKTFSSKGFDCS
Sbjct: 841 QHILAACRAYMEGTPVGSDYQRGTTKPENPNQSSTGFKIMLAKIFPRLIKTFSSKGFDCS 900

Query: 901 QFMEAENGFIL 911
           QFMEAENGFIL
Sbjct: 901 QFMEAENGFIL 911

BLAST of MELO3C007283.jh1 vs. NCBI nr
Match: XP_004134704.1 (probable ubiquitin-conjugating enzyme E2 24 [Cucumis sativus] >XP_031743225.1 probable ubiquitin-conjugating enzyme E2 24 [Cucumis sativus] >XP_031743226.1 probable ubiquitin-conjugating enzyme E2 24 [Cucumis sativus])

HSP 1 Score: 1800 bits (4661), Expect = 0.0
Identity = 882/911 (96.82%), Postives = 898/911 (98.57%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60
           MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD
Sbjct: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60

Query: 61  AVCFVNDPYGQMGKVVGIEMLVDLEKNSGPLIKNVNSKDLLKIRSISAGDVVVNGPWIGR 120
           AVCFVNDPYGQMGKVVGI+MLVDLEKNSGP+IKNVNSKDLLKIRSISAGDVVVNGPWIGR
Sbjct: 61  AVCFVNDPYGQMGKVVGIDMLVDLEKNSGPIIKNVNSKDLLKIRSISAGDVVVNGPWIGR 120

Query: 121 VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180
           VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVD LDDSQYPYYPGQRVRFSNVSKPPGW
Sbjct: 121 VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDVLDDSQYPYYPGQRVRFSNVSKPPGW 180

Query: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASILVGCSSSLTAPPRMQHAKELTLLSCFSHANW 240
           LSGWKDDRCEGTVCSVEAGLVYVDWLAS+L+GCSSSLTAPPRMQHAKELTLLSCFSHANW
Sbjct: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASVLMGCSSSLTAPPRMQHAKELTLLSCFSHANW 240

Query: 241 NLGDWCIFPGDEKNNYFGEKFNSTHDLMNGGYRGTGFPEICIIVKKKTKVDVLWQDGSRS 300
           NLGDWCIFPGDEKNNYFGEKF +THDLMNGGYRGTGF +IC+IVKKKTKVDVLWQDGSRS
Sbjct: 241 NLGDWCIFPGDEKNNYFGEKFITTHDLMNGGYRGTGFQDICVIVKKKTKVDVLWQDGSRS 300

Query: 301 TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGIVQAVDAKERTVKVQLR 360
           TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWG+VQAVDAKERTVKVQLR
Sbjct: 301 TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGVVQAVDAKERTVKVQLR 360

Query: 361 TNDLSEGSHLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLAMDKFASQSDENNIISEIS 420
           T+DLSEGS+LDGLLVEETMSAYELVEHPDYSFSLGDIVFRLA+DKFASQSDENNIISEIS
Sbjct: 361 TDDLSEGSNLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLALDKFASQSDENNIISEIS 420

Query: 421 MNESAQNEGNHIINNNNMNMDNFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK 480
           MNESAQNEGN IINNN M MDNFLSFIGNVTGFKDGAVEVKWANCS SMVSPQEIF IDK
Sbjct: 421 MNESAQNEGNRIINNN-MIMDNFLSFIGNVTGFKDGAVEVKWANCSISMVSPQEIFSIDK 480

Query: 481 YEVSSIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE 540
           YEVS IAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE
Sbjct: 481 YEVSPIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE 540

Query: 541 FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEIDLLCEKEVLQTCSQSTEMSQIAKLHN 600
           FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEI+LLCEKEVLQTCSQSTEMSQIAKLHN
Sbjct: 541 FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEINLLCEKEVLQTCSQSTEMSQIAKLHN 600

Query: 601 FGETSLDIEEIHKNEDLTFSTSSKNYDLLTQFDMASDCPDHHFLSSGKGSMLSQASRKWL 660
           FGETSLDIEEIHKNEDLTFSTSS+NYDLLTQFDMASDCPDHHFLSSGKG MLSQASR+WL
Sbjct: 601 FGETSLDIEEIHKNEDLTFSTSSENYDLLTQFDMASDCPDHHFLSSGKGPMLSQASRRWL 660

Query: 661 KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI 720
           KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI
Sbjct: 661 KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI 720

Query: 721 PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780
           PPLVHYISGGLRVNPNLYESG+VCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE
Sbjct: 721 PPLVHYISGGLRVNPNLYESGRVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780

Query: 781 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFETLVEEHFRRRY 840
           KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFE LVEEHFRRRY
Sbjct: 781 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFEALVEEHFRRRY 840

Query: 841 QHILAACRAYMEGTPVGSDYQRGTTKPENPNQSSTGFKIMLAKIFPRLIKTFSSKGFDCS 900
           QHILAACRAYMEGTPVGSDYQ GTTKPENP+QSSTGFKIMLAKIFPRLIKTFSSKGFDCS
Sbjct: 841 QHILAACRAYMEGTPVGSDYQCGTTKPENPDQSSTGFKIMLAKIFPRLIKTFSSKGFDCS 900

Query: 901 QFMEAENGFIL 911
           QFMEAENG IL
Sbjct: 901 QFMEAENGLIL 910

BLAST of MELO3C007283.jh1 vs. NCBI nr
Match: KAE8647689.1 (hypothetical protein Csa_003794 [Cucumis sativus])

HSP 1 Score: 1800 bits (4661), Expect = 0.0
Identity = 882/911 (96.82%), Postives = 898/911 (98.57%), Query Frame = 0

Query: 1    MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60
            MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD
Sbjct: 440  MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 499

Query: 61   AVCFVNDPYGQMGKVVGIEMLVDLEKNSGPLIKNVNSKDLLKIRSISAGDVVVNGPWIGR 120
            AVCFVNDPYGQMGKVVGI+MLVDLEKNSGP+IKNVNSKDLLKIRSISAGDVVVNGPWIGR
Sbjct: 500  AVCFVNDPYGQMGKVVGIDMLVDLEKNSGPIIKNVNSKDLLKIRSISAGDVVVNGPWIGR 559

Query: 121  VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180
            VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVD LDDSQYPYYPGQRVRFSNVSKPPGW
Sbjct: 560  VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDVLDDSQYPYYPGQRVRFSNVSKPPGW 619

Query: 181  LSGWKDDRCEGTVCSVEAGLVYVDWLASILVGCSSSLTAPPRMQHAKELTLLSCFSHANW 240
            LSGWKDDRCEGTVCSVEAGLVYVDWLAS+L+GCSSSLTAPPRMQHAKELTLLSCFSHANW
Sbjct: 620  LSGWKDDRCEGTVCSVEAGLVYVDWLASVLMGCSSSLTAPPRMQHAKELTLLSCFSHANW 679

Query: 241  NLGDWCIFPGDEKNNYFGEKFNSTHDLMNGGYRGTGFPEICIIVKKKTKVDVLWQDGSRS 300
            NLGDWCIFPGDEKNNYFGEKF +THDLMNGGYRGTGF +IC+IVKKKTKVDVLWQDGSRS
Sbjct: 680  NLGDWCIFPGDEKNNYFGEKFITTHDLMNGGYRGTGFQDICVIVKKKTKVDVLWQDGSRS 739

Query: 301  TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGIVQAVDAKERTVKVQLR 360
            TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWG+VQAVDAKERTVKVQLR
Sbjct: 740  TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGVVQAVDAKERTVKVQLR 799

Query: 361  TNDLSEGSHLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLAMDKFASQSDENNIISEIS 420
            T+DLSEGS+LDGLLVEETMSAYELVEHPDYSFSLGDIVFRLA+DKFASQSDENNIISEIS
Sbjct: 800  TDDLSEGSNLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLALDKFASQSDENNIISEIS 859

Query: 421  MNESAQNEGNHIINNNNMNMDNFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK 480
            MNESAQNEGN IINNN M MDNFLSFIGNVTGFKDGAVEVKWANCS SMVSPQEIF IDK
Sbjct: 860  MNESAQNEGNRIINNN-MIMDNFLSFIGNVTGFKDGAVEVKWANCSISMVSPQEIFSIDK 919

Query: 481  YEVSSIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE 540
            YEVS IAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE
Sbjct: 920  YEVSPIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE 979

Query: 541  FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEIDLLCEKEVLQTCSQSTEMSQIAKLHN 600
            FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEI+LLCEKEVLQTCSQSTEMSQIAKLHN
Sbjct: 980  FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEINLLCEKEVLQTCSQSTEMSQIAKLHN 1039

Query: 601  FGETSLDIEEIHKNEDLTFSTSSKNYDLLTQFDMASDCPDHHFLSSGKGSMLSQASRKWL 660
            FGETSLDIEEIHKNEDLTFSTSS+NYDLLTQFDMASDCPDHHFLSSGKG MLSQASR+WL
Sbjct: 1040 FGETSLDIEEIHKNEDLTFSTSSENYDLLTQFDMASDCPDHHFLSSGKGPMLSQASRRWL 1099

Query: 661  KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI 720
            KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI
Sbjct: 1100 KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI 1159

Query: 721  PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780
            PPLVHYISGGLRVNPNLYESG+VCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE
Sbjct: 1160 PPLVHYISGGLRVNPNLYESGRVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 1219

Query: 781  KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFETLVEEHFRRRY 840
            KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFE LVEEHFRRRY
Sbjct: 1220 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFEALVEEHFRRRY 1279

Query: 841  QHILAACRAYMEGTPVGSDYQRGTTKPENPNQSSTGFKIMLAKIFPRLIKTFSSKGFDCS 900
            QHILAACRAYMEGTPVGSDYQ GTTKPENP+QSSTGFKIMLAKIFPRLIKTFSSKGFDCS
Sbjct: 1280 QHILAACRAYMEGTPVGSDYQCGTTKPENPDQSSTGFKIMLAKIFPRLIKTFSSKGFDCS 1339

Query: 901  QFMEAENGFIL 911
            QFMEAENG IL
Sbjct: 1340 QFMEAENGLIL 1349

BLAST of MELO3C007283.jh1 vs. NCBI nr
Match: XP_038881614.1 (probable ubiquitin-conjugating enzyme E2 24 [Benincasa hispida])

HSP 1 Score: 1701 bits (4406), Expect = 0.0
Identity = 831/911 (91.22%), Postives = 867/911 (95.17%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60
           MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD
Sbjct: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60

Query: 61  AVCFVNDPYGQMGKVVGIEMLVDLEKNSGPLIKNVNSKDLLKIRSISAGDVVVNGPWIGR 120
           AVC VNDP+GQMGKVVGI+MLVDLEKN GPLIK+VNSK+LLKIRSISAGDVVVNGPWIGR
Sbjct: 61  AVCSVNDPFGQMGKVVGIDMLVDLEKNHGPLIKDVNSKNLLKIRSISAGDVVVNGPWIGR 120

Query: 121 VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180
           VDKVIDSVSVLFDDG KCEI  VDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW
Sbjct: 121 VDKVIDSVSVLFDDGTKCEITTVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180

Query: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASILVGCSSSLTAPPRMQHAKELTLLSCFSHANW 240
           LSGWKDDRCEGTVC VEAGLVYVDWLAS+L+GCSSSL APPR+QHAKEL  LSCF HANW
Sbjct: 181 LSGWKDDRCEGTVCYVEAGLVYVDWLASVLMGCSSSLMAPPRLQHAKELISLSCFPHANW 240

Query: 241 NLGDWCIFPGDEKNNYFGEKFNSTHDLMNGGYRGTGFPEICIIVKKKTKVDVLWQDGSRS 300
           NLGDWCIFP DEK+NYFGEK  +THDL+NGGYRGTGF EIC+IVKKKTKVDVLWQDGSRS
Sbjct: 241 NLGDWCIFPVDEKDNYFGEKLITTHDLINGGYRGTGFQEICVIVKKKTKVDVLWQDGSRS 300

Query: 301 TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGIVQAVDAKERTVKVQLR 360
           T LDS CLTPAN  NAHEFWPQQFVMEKGNCDDSSMSV QRWG+VQAVDAKERTVKVQ+R
Sbjct: 301 TGLDSHCLTPANVMNAHEFWPQQFVMEKGNCDDSSMSVYQRWGVVQAVDAKERTVKVQMR 360

Query: 361 TNDLSEGSHLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLAMDKFASQSDENNIISEIS 420
           T+DLSEGS+LDGLLVEETMSAYELVEHPDY+FSLGD+VFRLA+DKF SQSD+NNIISEIS
Sbjct: 361 TDDLSEGSNLDGLLVEETMSAYELVEHPDYNFSLGDVVFRLALDKFGSQSDKNNIISEIS 420

Query: 421 MNESAQNEGNHIINNNNMNMDNFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK 480
           MNESAQN  NHI++NN MNMDNFLSFIG+VTGFKDGAVEVKWANCSTSMVSPQEIFPIDK
Sbjct: 421 MNESAQNGKNHIVDNN-MNMDNFLSFIGHVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK 480

Query: 481 YEVSSIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE 540
           YE+S+ A PNGENLEESR +QIEPLKPYNIQKGKDMPNCDGA KNFKRDTTCFFSRAA+E
Sbjct: 481 YEISTTAEPNGENLEESRQDQIEPLKPYNIQKGKDMPNCDGAGKNFKRDTTCFFSRAAIE 540

Query: 541 FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEIDLLCEKEVLQTCSQSTEMSQIAKLHN 600
           FFSNIF+T FGSVRSTI+SGTFTALQSFEG DEIDLLCEKEVLQTCS+S EMSQIAK HN
Sbjct: 541 FFSNIFSTFFGSVRSTIISGTFTALQSFEGEDEIDLLCEKEVLQTCSESCEMSQIAKSHN 600

Query: 601 FGETSLDIEEIHKNEDLTFSTSSKNYDLLTQFDMASDCPDHHFLSSGKGSMLSQASRKWL 660
           FGETS +IEEIHK EDLT STSS+NYDL  QFDMASDCPDHHFLSSGKGS+LSQASR WL
Sbjct: 601 FGETS-NIEEIHKYEDLTLSTSSENYDLFAQFDMASDCPDHHFLSSGKGSILSQASRGWL 660

Query: 661 KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI 720
           KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDI+LPPEYPQI
Sbjct: 661 KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIFLPPEYPQI 720

Query: 721 PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780
           PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVW+PGSSTILQ+LLSLQALVLNE
Sbjct: 721 PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWSPGSSTILQILLSLQALVLNE 780

Query: 781 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFETLVEEHFRRRY 840
           KPYYNEAGYDAQLGR EGERNSASYNENAFLVTCKSMMYLLSK PKHFETLVEEHFRRRY
Sbjct: 781 KPYYNEAGYDAQLGRTEGERNSASYNENAFLVTCKSMMYLLSKPPKHFETLVEEHFRRRY 840

Query: 841 QHILAACRAYMEGTPVGSDYQRGTTKPENPNQSSTGFKIMLAKIFPRLIKTFSSKGFDCS 900
           QHILAACRAYMEGTPVGSDY RGTTK ENPNQSSTGFKIMLAKIFPRLIKTFSSKG DCS
Sbjct: 841 QHILAACRAYMEGTPVGSDYHRGTTKLENPNQSSTGFKIMLAKIFPRLIKTFSSKGIDCS 900

Query: 901 QFMEAENGFIL 911
           QF+E ENG IL
Sbjct: 901 QFVEVENGLIL 909

BLAST of MELO3C007283.jh1 vs. NCBI nr
Match: XP_022978639.1 (probable ubiquitin-conjugating enzyme E2 24 [Cucurbita maxima] >XP_022978640.1 probable ubiquitin-conjugating enzyme E2 24 [Cucurbita maxima] >XP_022978641.1 probable ubiquitin-conjugating enzyme E2 24 [Cucurbita maxima])

HSP 1 Score: 1593 bits (4125), Expect = 0.0
Identity = 787/906 (86.87%), Postives = 829/906 (91.50%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60
           MDL LSDSDWENSS+CDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREF+LGD
Sbjct: 1   MDLILSDSDWENSSDCDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFMLGD 60

Query: 61  AVCFVNDPYGQMGKVVGIEMLVDLEKNSGPLIKNVNSKDLLKIRSISAGDVVVNGPWIGR 120
            VC VNDP+GQMGKVVGI+MLVDLE N GP+IK+VNSKDL KIR IS GDVVV+G WIGR
Sbjct: 61  VVCSVNDPFGQMGKVVGIDMLVDLETNHGPIIKDVNSKDLRKIRPISVGDVVVHGKWIGR 120

Query: 121 VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180
           V+KVIDSVSVLFDDG KCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSN SKPP W
Sbjct: 121 VEKVIDSVSVLFDDGTKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNASKPPRW 180

Query: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASILVGCSSSLTAPPRMQHAKELTLLSCFSHANW 240
           LSGWKD+RCEGTVCSV+AGLVYVDWLASIL+GCSSSLTAPPRMQHAKELT+LSCF HANW
Sbjct: 181 LSGWKDNRCEGTVCSVDAGLVYVDWLASILMGCSSSLTAPPRMQHAKELTVLSCFPHANW 240

Query: 241 NLGDWCIFPGDEKNNYFGEKFNSTHDLMNGGYRGTGFPEICIIVKKKTKVDVLWQDGSRS 300
           +LGDWCIFP DEK++Y GEKF + HDL+ GGYR TG  EI +IVKKKTKVDVLWQDGS S
Sbjct: 241 HLGDWCIFPVDEKSDYLGEKFITAHDLLTGGYRDTGLQEISVIVKKKTKVDVLWQDGSHS 300

Query: 301 TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGIVQAVDAKERTVKVQLR 360
             LDS CLTPANA NAHEFWPQQFVMEKGNCDDSSMS  QRWG+VQAVDAKERTVKVQLR
Sbjct: 301 RGLDSHCLTPANAMNAHEFWPQQFVMEKGNCDDSSMSETQRWGVVQAVDAKERTVKVQLR 360

Query: 361 TNDLSEGSHLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLAMDKFASQSDENNIISEIS 420
           T DL+EGS LDGL VEETMSAYELVEHPDYSF+LGDIVFRLA   F SQSDENNI SEIS
Sbjct: 361 TGDLTEGSDLDGLWVEETMSAYELVEHPDYSFTLGDIVFRLA--SFGSQSDENNITSEIS 420

Query: 421 MNE-SAQNEGNHIINNNNMNMDNFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPID 480
           MNE SAQN  +HII+NN MNMDNFLSFIGNVT FKDG VEVKWANCS+SMVSP EIFPID
Sbjct: 421 MNEESAQNGESHIISNN-MNMDNFLSFIGNVTSFKDGTVEVKWANCSSSMVSPHEIFPID 480

Query: 481 KYEVSSIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAV 540
           KYEVS+IAVP+G+N+EE R +QIEP KP NIQKGKDMPN D A KNF+RDTTCF SRAA+
Sbjct: 481 KYEVSAIAVPSGDNVEELRQDQIEPSKPDNIQKGKDMPNFDAAGKNFRRDTTCFLSRAAI 540

Query: 541 EFFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEIDLLCEKEVLQTCSQSTEMSQIAKLH 600
           EFFSN+FTTLFGSVRSTI+SGTFTALQSFEGGD ID+L EKEVLQTCSQ T  SQIAKL 
Sbjct: 541 EFFSNVFTTLFGSVRSTIISGTFTALQSFEGGDNIDILPEKEVLQTCSQFTGRSQIAKLK 600

Query: 601 NFGETSLDIEEIHKNEDLTFSTSSKNYDLLTQFDMASDCPDHHFLSSGKGSMLSQASRKW 660
           +FGETSL+ EEIH+NE  T STSS+NYDL TQFDMASDCPDHHFLSSG  SMLSQA R W
Sbjct: 601 SFGETSLNTEEIHENEASTLSTSSENYDLFTQFDMASDCPDHHFLSSGNASMLSQARRGW 660

Query: 661 LKKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQ 720
           LKKVQQEWSILKNNLPETVYVRVFEERMDLIR+VIIGAPGTPYHDGLFFFDI+LPPEYPQ
Sbjct: 661 LKKVQQEWSILKNNLPETVYVRVFEERMDLIRSVIIGAPGTPYHDGLFFFDIFLPPEYPQ 720

Query: 721 IPPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLN 780
           IPPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLN
Sbjct: 721 IPPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLN 780

Query: 781 EKPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFETLVEEHFRRR 840
           EKPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSK PKHFE LVEEHFRRR
Sbjct: 781 EKPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKPPKHFEALVEEHFRRR 840

Query: 841 YQHILAACRAYMEGTPVGSDYQRGTTKPENPNQSSTGFKIMLAKIFPRLIKTFSSKGFDC 900
           YQHILAACRAYMEGTPVG+DY  G  K  NPNQSSTGFKIMLAKI+PRLIKTFSSKG DC
Sbjct: 841 YQHILAACRAYMEGTPVGADYHSGAKKLVNPNQSSTGFKIMLAKIYPRLIKTFSSKGIDC 900

Query: 901 SQFMEA 905
           SQF EA
Sbjct: 901 SQFFEA 903

BLAST of MELO3C007283.jh1 vs. ExPASy Swiss-Prot
Match: Q8VY10 (Probable ubiquitin-conjugating enzyme E2 24 OS=Arabidopsis thaliana OX=3702 GN=UBC24 PE=1 SV=1)

HSP 1 Score: 842.4 bits (2175), Expect = 4.7e-243
Identity = 443/928 (47.74%), Postives = 606/928 (65.30%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60
           M++ L+DSDW++SS+  SS+  E    Y G+   I S+LEETIGKIDEFLSFER F+ GD
Sbjct: 1   MEMSLTDSDWDSSSDSGSSEHEEVEFSYGGRAQNIFSNLEETIGKIDEFLSFERGFMYGD 60

Query: 61  AVCFVNDPYGQMGKVVGIEMLVDLEKNSGPLIKNVNSKDLLKIRSISAGDVVVNGPWIGR 120
            V    +P GQ G+V+ I+M V+LE   G ++K V++K L K+RSIS  D V+NGPW+GR
Sbjct: 61  IVRSATEPSGQSGRVINIDMFVNLESTHGKIMKEVDTKRLQKLRSISLSDYVINGPWVGR 120

Query: 121 VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDALDDSQYPYYPGQR--VRFSNVSKPP 180
           VDK+++ VSV  DDG   E++   Q+KL+ I  + L+DSQY YYPGQR  V+ ++  +  
Sbjct: 121 VDKIVERVSVTLDDGTNYEVLVDGQDKLVAIPPNLLEDSQYSYYPGQRVQVKLAHAPRST 180

Query: 181 GWLSG-WKDDRCEGTVCSVEAGLVYVDWLASILVGCSSSLTAPPRMQHAKELTLLSCFSH 240
            WL G W+  +  GTVC+VEAGLVYVDW+ASI++    +LTAP  +Q+ + LTLL C SH
Sbjct: 181 TWLCGTWRGTQVMGTVCTVEAGLVYVDWVASIVMEGDRNLTAPQALQNPESLTLLPCVSH 240

Query: 241 ANWNLGDWCIFPGD--------EKNNYFGEKFNSTHDLMNGGY----RGTGFPEICIIVK 300
           A+W LGDWCI PG         +  N      N  H     G+    + +G  E+ +I K
Sbjct: 241 ASWQLGDWCILPGSSHCDIAERQTPNVAAYNLNECHKTFQKGFNRNMQNSGLDELFVITK 300

Query: 301 KKTKVDVLWQDGSRSTALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGIV 360
            K KV V+WQDGS S  +DS  L P  A NAH+FWP+QFV+EK  C+      +++WG+V
Sbjct: 301 TKMKVAVMWQDGSCSLGVDSQQLLPVGAVNAHDFWPEQFVVEKETCN------SKKWGVV 360

Query: 361 QAVDAKERTVKVQLRTNDLSEGSHLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLAMD- 420
           +AV+AKE+TVKVQ       E +     ++EE +SAYEL+EHPD+ F   D+V +L  + 
Sbjct: 361 KAVNAKEQTVKVQWTIQVEKEATGCVDEVMEEIVSAYELLEHPDFGFCFSDVVVKLLPEG 420

Query: 421 KFASQSDENNIISEISMNESAQNEGNHIINNNNMNMDNFLSFIGNVTGFKDGAVEVKWAN 480
           KF   +D   I++          E  H++  ++ +   FLS IG VTGFK+G+V+VKWAN
Sbjct: 421 KFDPNAD--TIVA---------TEAKHLLTESDYSGAYFLSSIGVVTGFKNGSVKVKWAN 480

Query: 481 CSTSMVSPQEIFPIDKYEVSSIAVPNGENLEE---SRLEQIEPLKPYNIQKG--KDMPNC 540
            STS V+P EI+ +++ E S+ +  + E   +    ++ Q +     + + G  K     
Sbjct: 481 GSTSKVAPCEIWKMERSEYSNSSTVSSEGSVQDLSQKISQSDEASSNHQETGLVKLYSVG 540

Query: 541 DGASKNFKRDTTCFFSRAAVEFFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEIDLLCE 600
           +  ++N    ++ F  +AA+ F +N+ ++LFG   ST +  + +     E   + ++L +
Sbjct: 541 ESCNENIPECSSFFLPKAAIGFITNLASSLFGYQGSTSVISSHSRCNDSEDQSDSEVLVQ 600

Query: 601 KEVLQTCSQSTEMSQIAKLHNFGETSLDIEEIHKNEDLTFSTSSKNYDLLTQFDMASDCP 660
           +      +  T   ++          ++ + I+K  D T   +S+N     QFDM +DC 
Sbjct: 601 ETAESYDNSETNSGEVDMTTTMVNIPIEGKGINKTLDSTLLENSRNQVRFRQFDMVNDCS 660

Query: 661 DHHFLSSGKGSMLSQASRKWLKKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPG 720
           DHHFLSS KG   SQ ++ W+KKVQQEWS L+ NLP T+YVRV EERMDL+RA ++GAPG
Sbjct: 661 DHHFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNTIYVRVCEERMDLLRAALVGAPG 720

Query: 721 TPYHDGLFFFDIYLPPEYPQIPPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVW 780
           TPYHDGLFFFDI LPP+YP  PP+VHY SGG+R+NPNLYESG+VCLSLLNTW+GSG+EVW
Sbjct: 721 TPYHDGLFFFDIMLPPQYPHEPPMVHYHSGGMRLNPNLYESGRVCLSLLNTWSGSGTEVW 780

Query: 781 NPGSSTILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMY 840
           N GSS+ILQ+LLS QALVLNEKPY+NEAGYD QLGRAEGE+NS SYNENAFL+TCKSM+ 
Sbjct: 781 NAGSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAEGEKNSVSYNENAFLITCKSMIS 840

Query: 841 LLSKSPKHFETLVEEHFRRRYQHILAACRAYMEGTPVGSDYQRGTTKPENPNQSSTGFKI 900
           +L K PKHFE LV++HF  R QH+LAAC+AYMEG PVGS          N   +STGFKI
Sbjct: 841 MLRKPPKHFEMLVKDHFTHRAQHVLAACKAYMEGVPVGS----SANLQGNSTTNSTGFKI 900

Query: 901 MLAKIFPRLIKTFSSKGFDCSQFMEAEN 908
           ML+K++P+L++ FS  G DC Q +  E+
Sbjct: 901 MLSKLYPKLLEAFSEIGVDCVQEIGPES 907

BLAST of MELO3C007283.jh1 vs. ExPASy Swiss-Prot
Match: Q9ZVX1 (Probable ubiquitin-conjugating enzyme E2 23 OS=Arabidopsis thaliana OX=3702 GN=UBC23 PE=1 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 6.3e-171
Identity = 364/984 (36.99%), Postives = 530/984 (53.86%), Query Frame = 0

Query: 41   ETIGKIDEFLSFEREFLLGDAVCFVNDPYGQMGKVVGIEMLVDLEKNSGPLIKNVNSKDL 100
            E +  I++    +R FL GD V   ++P GQ+G VV + + VDL    G + K++++K+L
Sbjct: 130  EPVQDINDVTVIDRGFLHGDYVASASEPTGQVGVVVDVNISVDLLAPDGSIHKDISTKNL 189

Query: 101  LKIRSISAGDVVVNGPWIGRVDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDAL-DDS 160
             ++R  + GD VV+GPW+GR+D V+D+V+VLFDDG+ C+++ V+  +L PI  + L +D+
Sbjct: 190  KRVRDFAVGDYVVHGPWLGRIDDVLDNVTVLFDDGSMCKVLRVEPLRLKPIPKNNLEEDA 249

Query: 161  QYPYYPGQRVR--FSNVSKPPGWLSG-WKDDRCEGTVCSVEAGLVYVDWLASILVGCSSS 220
             +PYYPGQRV+   S+V K   WLSG WK +R EGTV  V AG ++V W+AS   G  SS
Sbjct: 250  NFPYYPGQRVKASSSSVFKNSRWLSGLWKPNRLEGTVTKVTAGSIFVYWIASAGFGPDSS 309

Query: 221  LTAPPRMQHAKELTLLSCFSHANWNLGDWCIFPG----------------------DEKN 280
            + +PP  Q+   LTLLSCF+HANW +GDWC+ P                        ++ 
Sbjct: 310  V-SPPEEQNPSNLTLLSCFTHANWQVGDWCLLPSLNQSATIPLHKHVSKLRLYDSQADRQ 369

Query: 281  NYFGEKFNSTHDLMNGGYRGTG-------------------------------------- 340
               G       D ++G     G                                      
Sbjct: 370  QKIGRDLEDVQDEVSGKVEPAGITAEALPKVTSDDPPQRNPSVSKEPVHEPWPLHRKKIR 429

Query: 341  ----------------FPEICIIVKKKTKVDVLWQDGSRSTALDSPCLTPANATNAHEFW 400
                            F +  ++V  +T+VDV WQDG+     ++  L P      HEF 
Sbjct: 430  KLVIRKDKKVKKKEESFEQALLVVNSRTRVDVSWQDGTIECRREAITLIPIETPGDHEFV 489

Query: 401  PQQFVMEKGNCDDSSMSVNQRWGIVQAVDAKERTVKVQLRTNDLSEGSHLDGLLVEETMS 460
             +Q+V+EK + D  + +  +R G+V+ V+AK+RT  V+   N L           EE +S
Sbjct: 490  SEQYVVEKTSDDGDNTTEPRRAGVVKNVNAKDRTASVRW-LNPLRRAEEPREFEKEEIVS 549

Query: 461  AYELVEHPDYSFSLGDIVFRLAMDKFA--SQSDENNIISEISMNESAQNEGNH----IIN 520
             YEL  HPDY +  GD+V RL+    A  + S  N+       +   Q+  +H    I+ 
Sbjct: 550  VYELEGHPDYDYCYGDVVVRLSPIAVALPASSPGNSFEEATQQDNGYQDSESHQEAKILV 609

Query: 521  NNNMNMDNF----LSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDKYEVSSIAVPN 580
            +   N  +     LS++GN+TG KDG +EV WA+ + S V P  ++ + + +        
Sbjct: 610  DKEENEPSTDLSKLSWVGNITGLKDGDIEVTWADGTISTVGPHAVYVVGRDDDDESVAGE 669

Query: 581  GENLEESRLEQIE-------PLKPYNIQKGKDMP-NCDG---ASKNFKRDTTCFFSRAAV 640
             E  + +  E +         +   ++ +   +  N D    A  +  R+       AA+
Sbjct: 670  SETSDAASWETLNDDDRGAPEIPEEDLGRSSSIEGNSDADIYAENDSGRNGALALPLAAI 729

Query: 641  EFFSNIFTTLFGSVRSTI--------LSGTFTALQSFEGGDEIDLL-----CEKEVLQTC 700
            EF + + + +F   R ++        +   +   +S    DE D L      +  V   C
Sbjct: 730  EFVTRLASGIFSRARKSVDSSSSDYTVENVYKQAESTNPSDETDSLDDPSPSKVNVTDNC 789

Query: 701  -SQSTEMSQIAKLHNFGETSLDIEEIHKNEDLTFSTSSKNYDLLTQFDMASDCPDHHFLS 760
             S+ T+ +  AK    GETS  +E+    ED    +   +     +FD++ D  DHHFL 
Sbjct: 790  ESKGTQAN--AKNILSGETSTFLED----EDKPVPSEGDSCS-FRRFDISQDPLDHHFLG 849

Query: 761  -SGKGSMLSQASRKWLKKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHD 820
              G+        R+W KKV Q+W IL+NNLP+ ++VR +E+RMDL+RAVI+GA GTPY D
Sbjct: 850  VDGQ----KTKERQWFKKVDQDWKILQNNLPDGIFVRAYEDRMDLLRAVIVGAFGTPYQD 909

Query: 821  GLFFFDIYLPPEYPQIPPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSS 880
            GLFFFD +LP +YP +PP  +Y SGG R+NPNLYE GKVCLSLLNTWTG G+EVW+P SS
Sbjct: 910  GLFFFDFHLPSDYPSVPPSAYYHSGGWRLNPNLYEEGKVCLSLLNTWTGRGNEVWDPKSS 969

Query: 881  TILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKS 907
            +ILQVL+SLQ LVLN KPY+NEAGYD Q+G AEGE+NS  YNEN FL+ CK+MMYL+ K 
Sbjct: 970  SILQVLVSLQGLVLNSKPYFNEAGYDKQVGTAEGEKNSLGYNENTFLLNCKTMMYLMRKP 1029

BLAST of MELO3C007283.jh1 vs. ExPASy Swiss-Prot
Match: Q8GY87 (Probable ubiquitin-conjugating enzyme E2 26 OS=Arabidopsis thaliana OX=3702 GN=UBC26 PE=2 SV=2)

HSP 1 Score: 288.5 bits (737), Expect = 2.6e-76
Identity = 142/267 (53.18%), Postives = 175/267 (65.54%), Query Frame = 0

Query: 631 QFDMASDCPDHHFLSSGKGSMLSQASRKWLKKVQQEWSILKNNLPETVYVRVFEERMDLI 690
           +FD   D  DHH+ S GK S   Q S+ W+KKVQ +W IL+N+LPE + VR  E RMDL+
Sbjct: 246 RFDTVEDFSDHHYASKGKSS--KQHSKNWVKKVQADWKILENDLPEAISVRACESRMDLL 305

Query: 691 RAVIIGAPGTPYHDGLFFFDIYLPPEYPQIPPLVHYISGGLRVNPNLYESGKVCLSLLNT 750
           RAVIIGA GTPYHDGLFFFDI  P  YP +PP VHY SGGLR+NPNLY  GKVCLSLL T
Sbjct: 306 RAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVHYHSGGLRINPNLYNCGKVCLSLLGT 365

Query: 751 WTGSGSEVWNPGSSTILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSASYNENAF 810
           W GS  E W P  ST+LQ+L+S+QAL+LNEKPY+NE GY    G A GE  S  Y+EN F
Sbjct: 366 WAGSAREKWLPNESTMLQLLVSIQALILNEKPYFNEPGYVQSAGTASGESKSKVYSENVF 425

Query: 811 LVTCKSMMYLLSKSPKHFETLVEEHFRRRYQHILAACRAYMEGTPVGSDYQRGTTKPENP 870
           L++ K+M+Y + + P+HFE  V+ H+  R   I+ AC AY  G P+GS  + G    E  
Sbjct: 426 LLSLKTMVYSIRRPPQHFEEYVQNHYFVRSHDIVKACNAYKAGAPLGSMVKGGVQDLEEA 485

Query: 871 NQS-STGFKIMLAKIFPRLIKTFSSKG 897
            QS S  FK  +A     ++  F   G
Sbjct: 486 RQSGSKKFKTDVASFMQTVVDEFVKLG 510

BLAST of MELO3C007283.jh1 vs. ExPASy Swiss-Prot
Match: Q9C918 (Putative ubiquitin-conjugating enzyme E2 38 OS=Arabidopsis thaliana OX=3702 GN=UBC38 PE=3 SV=2)

HSP 1 Score: 284.3 bits (726), Expect = 5.0e-75
Identity = 142/267 (53.18%), Postives = 175/267 (65.54%), Query Frame = 0

Query: 631 QFDMASDCPDHHFLSSGKGSMLSQASRKWLKKVQQEWSILKNNLPETVYVRVFEERMDLI 690
           +FD   D  DHH++S GK S   Q S+ W+KKVQ EW IL  NLPET++VR  E RMDL+
Sbjct: 29  RFDSVEDFSDHHYVSKGKAS--KQHSKNWVKKVQDEWKILNQNLPETIFVRACESRMDLL 88

Query: 691 RAVIIGAPGTPYHDGLFFFDIYLPPEYPQIPPLVHYISGGLRVNPNLYESGKVCLSLLNT 750
           RAVIIGA GTPYHDGLFFFDI  P  YP +PP V+Y SGGLR+NPNLY  GKVCLSLL T
Sbjct: 89  RAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVYYHSGGLRINPNLYNCGKVCLSLLGT 148

Query: 751 WTGSGSEVWNPGSSTILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSASYNENAF 810
           W G+  + W P  ST+LQVL+S+QALVLNE+PY+NE GY    G   GE  S  Y+EN F
Sbjct: 149 WHGNARQSWLPKESTMLQVLVSIQALVLNEQPYFNEPGYGLIKGTWLGESKSKVYSENVF 208

Query: 811 LVTCKSMMYLLSKSPKHFETLVEEHFRRRYQHILAACRAYMEGTPVGSDYQRGTTKPENP 870
           L++ K+M+Y + K P+HFE  V+ H+  R   I+ AC AY  G P+GS  + G    E  
Sbjct: 209 LLSLKTMVYSMRKPPQHFEEYVQNHYFVRSHDIVKACNAYKAGAPLGSMVKGGVQDLEQA 268

Query: 871 NQS-STGFKIMLAKIFPRLIKTFSSKG 897
            QS S  FK  +A     ++  F   G
Sbjct: 269 RQSGSKKFKTDVASFMQTVVDEFVKLG 293

BLAST of MELO3C007283.jh1 vs. ExPASy Swiss-Prot
Match: Q9LUQ5 (Probable ubiquitin-conjugating enzyme E2 25 OS=Arabidopsis thaliana OX=3702 GN=UBC25 PE=2 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 2.0e-71
Identity = 124/232 (53.45%), Postives = 161/232 (69.40%), Query Frame = 0

Query: 626 YDLLTQFDMASDCPDHHFLSSGKGSMLSQASRKWLKKVQQEWSILKNNLPETVYVRVFEE 685
           Y    +FD+  D  DHH+ S  KG+   Q S+ W K++Q EW IL+ +LPE ++VR +E 
Sbjct: 302 YLFFKRFDIVEDFSDHHYAS--KGTTSKQHSKDWAKRIQDEWRILEKDLPEMIFVRAYES 361

Query: 686 RMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQIPPLVHYISGGLRVNPNLYESGKVCL 745
           RMDL+RAVIIGA GTPYHDGLFFFDI+ P  YP  PP+VHY SGGLR+NPNLY  GKVCL
Sbjct: 362 RMDLLRAVIIGAQGTPYHDGLFFFDIFFPDTYPSTPPIVHYHSGGLRINPNLYNCGKVCL 421

Query: 746 SLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSASY 805
           SLL TW+G+  E W P +ST+LQVL+S+Q L+LN+KPY+NE GY+   G A GE  S +Y
Sbjct: 422 SLLGTWSGNQREKWIPNTSTMLQVLVSIQGLILNQKPYFNEPGYERSAGSAHGESTSKAY 481

Query: 806 NENAFLVTCKSMMYLLSKSPKHFETLVEEHFRRRYQHILAACRAYMEGTPVG 858
           +EN F+++ K+M+Y + + PK+FE     HF      +L AC AY  G   G
Sbjct: 482 SENTFILSLKTMVYTMRRPPKYFEDFAYGHFFSCAHDVLKACNAYRNGATPG 531

BLAST of MELO3C007283.jh1 vs. ExPASy TrEMBL
Match: A0A1S3B0C7 (probable ubiquitin-conjugating enzyme E2 24 OS=Cucumis melo OX=3656 GN=LOC103484521 PE=4 SV=1)

HSP 1 Score: 1856 bits (4807), Expect = 0.0
Identity = 909/911 (99.78%), Postives = 911/911 (100.00%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60
           MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD
Sbjct: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60

Query: 61  AVCFVNDPYGQMGKVVGIEMLVDLEKNSGPLIKNVNSKDLLKIRSISAGDVVVNGPWIGR 120
           AVCFVNDPYGQMGKVVGIEMLVDLEKNSGPLIKNVNSKDLLKIRSISAGDVVVNGPWIGR
Sbjct: 61  AVCFVNDPYGQMGKVVGIEMLVDLEKNSGPLIKNVNSKDLLKIRSISAGDVVVNGPWIGR 120

Query: 121 VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180
           VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW
Sbjct: 121 VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180

Query: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASILVGCSSSLTAPPRMQHAKELTLLSCFSHANW 240
           LSGWKDDRCEGTVCSVEAGLVYVDWLASIL+GCSSSLTAPPRMQHAKELTLLSCFSHANW
Sbjct: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASILMGCSSSLTAPPRMQHAKELTLLSCFSHANW 240

Query: 241 NLGDWCIFPGDEKNNYFGEKFNSTHDLMNGGYRGTGFPEICIIVKKKTKVDVLWQDGSRS 300
           NLGDWCIFPGDEKNNYFGEKFNSTHDLMNGGYRGTGFPEICIIVKKKTKVDVLWQDGSRS
Sbjct: 241 NLGDWCIFPGDEKNNYFGEKFNSTHDLMNGGYRGTGFPEICIIVKKKTKVDVLWQDGSRS 300

Query: 301 TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGIVQAVDAKERTVKVQLR 360
           TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWG+VQAVDAKERTVKVQLR
Sbjct: 301 TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGVVQAVDAKERTVKVQLR 360

Query: 361 TNDLSEGSHLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLAMDKFASQSDENNIISEIS 420
           TNDLSEGSHLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLAMDKFASQSDENNIISEIS
Sbjct: 361 TNDLSEGSHLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLAMDKFASQSDENNIISEIS 420

Query: 421 MNESAQNEGNHIINNNNMNMDNFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK 480
           MNESAQNEGNHIINNNNMNMDNFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK
Sbjct: 421 MNESAQNEGNHIINNNNMNMDNFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK 480

Query: 481 YEVSSIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE 540
           YEVSSIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE
Sbjct: 481 YEVSSIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE 540

Query: 541 FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEIDLLCEKEVLQTCSQSTEMSQIAKLHN 600
           FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEIDLLCEKEVLQTCSQSTEMSQIAKLHN
Sbjct: 541 FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEIDLLCEKEVLQTCSQSTEMSQIAKLHN 600

Query: 601 FGETSLDIEEIHKNEDLTFSTSSKNYDLLTQFDMASDCPDHHFLSSGKGSMLSQASRKWL 660
           FGETSLDIEEIHKNEDLTFSTSSKNYDLLTQFDMASDCPDHHFLSSGKGSMLSQASRKWL
Sbjct: 601 FGETSLDIEEIHKNEDLTFSTSSKNYDLLTQFDMASDCPDHHFLSSGKGSMLSQASRKWL 660

Query: 661 KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI 720
           KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI
Sbjct: 661 KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI 720

Query: 721 PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780
           PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE
Sbjct: 721 PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780

Query: 781 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFETLVEEHFRRRY 840
           KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFETLVEEHFRRRY
Sbjct: 781 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFETLVEEHFRRRY 840

Query: 841 QHILAACRAYMEGTPVGSDYQRGTTKPENPNQSSTGFKIMLAKIFPRLIKTFSSKGFDCS 900
           QHILAACRAYMEGTPVGSDYQRGTTKPENPNQSSTGFKIMLAKIFPRLIKTFSSKGFDCS
Sbjct: 841 QHILAACRAYMEGTPVGSDYQRGTTKPENPNQSSTGFKIMLAKIFPRLIKTFSSKGFDCS 900

Query: 901 QFMEAENGFIL 911
           QFMEAENGFIL
Sbjct: 901 QFMEAENGFIL 911

BLAST of MELO3C007283.jh1 vs. ExPASy TrEMBL
Match: A0A5D3CMG4 (Putative ubiquitin-conjugating enzyme E2 24 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007470 PE=4 SV=1)

HSP 1 Score: 1856 bits (4807), Expect = 0.0
Identity = 909/911 (99.78%), Postives = 911/911 (100.00%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60
           MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD
Sbjct: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60

Query: 61  AVCFVNDPYGQMGKVVGIEMLVDLEKNSGPLIKNVNSKDLLKIRSISAGDVVVNGPWIGR 120
           AVCFVNDPYGQMGKVVGIEMLVDLEKNSGPLIKNVNSKDLLKIRSISAGDVVVNGPWIGR
Sbjct: 61  AVCFVNDPYGQMGKVVGIEMLVDLEKNSGPLIKNVNSKDLLKIRSISAGDVVVNGPWIGR 120

Query: 121 VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180
           VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW
Sbjct: 121 VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180

Query: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASILVGCSSSLTAPPRMQHAKELTLLSCFSHANW 240
           LSGWKDDRCEGTVCSVEAGLVYVDWLASIL+GCSSSLTAPPRMQHAKELTLLSCFSHANW
Sbjct: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASILMGCSSSLTAPPRMQHAKELTLLSCFSHANW 240

Query: 241 NLGDWCIFPGDEKNNYFGEKFNSTHDLMNGGYRGTGFPEICIIVKKKTKVDVLWQDGSRS 300
           NLGDWCIFPGDEKNNYFGEKFNSTHDLMNGGYRGTGFPEICIIVKKKTKVDVLWQDGSRS
Sbjct: 241 NLGDWCIFPGDEKNNYFGEKFNSTHDLMNGGYRGTGFPEICIIVKKKTKVDVLWQDGSRS 300

Query: 301 TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGIVQAVDAKERTVKVQLR 360
           TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWG+VQAVDAKERTVKVQLR
Sbjct: 301 TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGVVQAVDAKERTVKVQLR 360

Query: 361 TNDLSEGSHLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLAMDKFASQSDENNIISEIS 420
           TNDLSEGSHLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLAMDKFASQSDENNIISEIS
Sbjct: 361 TNDLSEGSHLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLAMDKFASQSDENNIISEIS 420

Query: 421 MNESAQNEGNHIINNNNMNMDNFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK 480
           MNESAQNEGNHIINNNNMNMDNFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK
Sbjct: 421 MNESAQNEGNHIINNNNMNMDNFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK 480

Query: 481 YEVSSIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE 540
           YEVSSIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE
Sbjct: 481 YEVSSIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE 540

Query: 541 FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEIDLLCEKEVLQTCSQSTEMSQIAKLHN 600
           FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEIDLLCEKEVLQTCSQSTEMSQIAKLHN
Sbjct: 541 FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEIDLLCEKEVLQTCSQSTEMSQIAKLHN 600

Query: 601 FGETSLDIEEIHKNEDLTFSTSSKNYDLLTQFDMASDCPDHHFLSSGKGSMLSQASRKWL 660
           FGETSLDIEEIHKNEDLTFSTSSKNYDLLTQFDMASDCPDHHFLSSGKGSMLSQASRKWL
Sbjct: 601 FGETSLDIEEIHKNEDLTFSTSSKNYDLLTQFDMASDCPDHHFLSSGKGSMLSQASRKWL 660

Query: 661 KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI 720
           KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI
Sbjct: 661 KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI 720

Query: 721 PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780
           PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE
Sbjct: 721 PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780

Query: 781 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFETLVEEHFRRRY 840
           KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFETLVEEHFRRRY
Sbjct: 781 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFETLVEEHFRRRY 840

Query: 841 QHILAACRAYMEGTPVGSDYQRGTTKPENPNQSSTGFKIMLAKIFPRLIKTFSSKGFDCS 900
           QHILAACRAYMEGTPVGSDYQRGTTKPENPNQSSTGFKIMLAKIFPRLIKTFSSKGFDCS
Sbjct: 841 QHILAACRAYMEGTPVGSDYQRGTTKPENPNQSSTGFKIMLAKIFPRLIKTFSSKGFDCS 900

Query: 901 QFMEAENGFIL 911
           QFMEAENGFIL
Sbjct: 901 QFMEAENGFIL 911

BLAST of MELO3C007283.jh1 vs. ExPASy TrEMBL
Match: A0A0A0KLH0 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G517350 PE=4 SV=1)

HSP 1 Score: 1800 bits (4661), Expect = 0.0
Identity = 882/911 (96.82%), Postives = 898/911 (98.57%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60
           MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD
Sbjct: 46  MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 105

Query: 61  AVCFVNDPYGQMGKVVGIEMLVDLEKNSGPLIKNVNSKDLLKIRSISAGDVVVNGPWIGR 120
           AVCFVNDPYGQMGKVVGI+MLVDLEKNSGP+IKNVNSKDLLKIRSISAGDVVVNGPWIGR
Sbjct: 106 AVCFVNDPYGQMGKVVGIDMLVDLEKNSGPIIKNVNSKDLLKIRSISAGDVVVNGPWIGR 165

Query: 121 VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180
           VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVD LDDSQYPYYPGQRVRFSNVSKPPGW
Sbjct: 166 VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDVLDDSQYPYYPGQRVRFSNVSKPPGW 225

Query: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASILVGCSSSLTAPPRMQHAKELTLLSCFSHANW 240
           LSGWKDDRCEGTVCSVEAGLVYVDWLAS+L+GCSSSLTAPPRMQHAKELTLLSCFSHANW
Sbjct: 226 LSGWKDDRCEGTVCSVEAGLVYVDWLASVLMGCSSSLTAPPRMQHAKELTLLSCFSHANW 285

Query: 241 NLGDWCIFPGDEKNNYFGEKFNSTHDLMNGGYRGTGFPEICIIVKKKTKVDVLWQDGSRS 300
           NLGDWCIFPGDEKNNYFGEKF +THDLMNGGYRGTGF +IC+IVKKKTKVDVLWQDGSRS
Sbjct: 286 NLGDWCIFPGDEKNNYFGEKFITTHDLMNGGYRGTGFQDICVIVKKKTKVDVLWQDGSRS 345

Query: 301 TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGIVQAVDAKERTVKVQLR 360
           TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWG+VQAVDAKERTVKVQLR
Sbjct: 346 TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGVVQAVDAKERTVKVQLR 405

Query: 361 TNDLSEGSHLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLAMDKFASQSDENNIISEIS 420
           T+DLSEGS+LDGLLVEETMSAYELVEHPDYSFSLGDIVFRLA+DKFASQSDENNIISEIS
Sbjct: 406 TDDLSEGSNLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLALDKFASQSDENNIISEIS 465

Query: 421 MNESAQNEGNHIINNNNMNMDNFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDK 480
           MNESAQNEGN IINNN M MDNFLSFIGNVTGFKDGAVEVKWANCS SMVSPQEIF IDK
Sbjct: 466 MNESAQNEGNRIINNN-MIMDNFLSFIGNVTGFKDGAVEVKWANCSISMVSPQEIFSIDK 525

Query: 481 YEVSSIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE 540
           YEVS IAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE
Sbjct: 526 YEVSPIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAVE 585

Query: 541 FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEIDLLCEKEVLQTCSQSTEMSQIAKLHN 600
           FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEI+LLCEKEVLQTCSQSTEMSQIAKLHN
Sbjct: 586 FFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEINLLCEKEVLQTCSQSTEMSQIAKLHN 645

Query: 601 FGETSLDIEEIHKNEDLTFSTSSKNYDLLTQFDMASDCPDHHFLSSGKGSMLSQASRKWL 660
           FGETSLDIEEIHKNEDLTFSTSS+NYDLLTQFDMASDCPDHHFLSSGKG MLSQASR+WL
Sbjct: 646 FGETSLDIEEIHKNEDLTFSTSSENYDLLTQFDMASDCPDHHFLSSGKGPMLSQASRRWL 705

Query: 661 KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI 720
           KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI
Sbjct: 706 KKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQI 765

Query: 721 PPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 780
           PPLVHYISGGLRVNPNLYESG+VCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE
Sbjct: 766 PPLVHYISGGLRVNPNLYESGRVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNE 825

Query: 781 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFETLVEEHFRRRY 840
           KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFE LVEEHFRRRY
Sbjct: 826 KPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFEALVEEHFRRRY 885

Query: 841 QHILAACRAYMEGTPVGSDYQRGTTKPENPNQSSTGFKIMLAKIFPRLIKTFSSKGFDCS 900
           QHILAACRAYMEGTPVGSDYQ GTTKPENP+QSSTGFKIMLAKIFPRLIKTFSSKGFDCS
Sbjct: 886 QHILAACRAYMEGTPVGSDYQCGTTKPENPDQSSTGFKIMLAKIFPRLIKTFSSKGFDCS 945

Query: 901 QFMEAENGFIL 911
           QFMEAENG IL
Sbjct: 946 QFMEAENGLIL 955

BLAST of MELO3C007283.jh1 vs. ExPASy TrEMBL
Match: A0A6J1IQQ9 (probable ubiquitin-conjugating enzyme E2 24 OS=Cucurbita maxima OX=3661 GN=LOC111478555 PE=4 SV=1)

HSP 1 Score: 1593 bits (4125), Expect = 0.0
Identity = 787/906 (86.87%), Postives = 829/906 (91.50%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60
           MDL LSDSDWENSS+CDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREF+LGD
Sbjct: 1   MDLILSDSDWENSSDCDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFMLGD 60

Query: 61  AVCFVNDPYGQMGKVVGIEMLVDLEKNSGPLIKNVNSKDLLKIRSISAGDVVVNGPWIGR 120
            VC VNDP+GQMGKVVGI+MLVDLE N GP+IK+VNSKDL KIR IS GDVVV+G WIGR
Sbjct: 61  VVCSVNDPFGQMGKVVGIDMLVDLETNHGPIIKDVNSKDLRKIRPISVGDVVVHGKWIGR 120

Query: 121 VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPGW 180
           V+KVIDSVSVLFDDG KCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSN SKPP W
Sbjct: 121 VEKVIDSVSVLFDDGTKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNASKPPRW 180

Query: 181 LSGWKDDRCEGTVCSVEAGLVYVDWLASILVGCSSSLTAPPRMQHAKELTLLSCFSHANW 240
           LSGWKD+RCEGTVCSV+AGLVYVDWLASIL+GCSSSLTAPPRMQHAKELT+LSCF HANW
Sbjct: 181 LSGWKDNRCEGTVCSVDAGLVYVDWLASILMGCSSSLTAPPRMQHAKELTVLSCFPHANW 240

Query: 241 NLGDWCIFPGDEKNNYFGEKFNSTHDLMNGGYRGTGFPEICIIVKKKTKVDVLWQDGSRS 300
           +LGDWCIFP DEK++Y GEKF + HDL+ GGYR TG  EI +IVKKKTKVDVLWQDGS S
Sbjct: 241 HLGDWCIFPVDEKSDYLGEKFITAHDLLTGGYRDTGLQEISVIVKKKTKVDVLWQDGSHS 300

Query: 301 TALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGIVQAVDAKERTVKVQLR 360
             LDS CLTPANA NAHEFWPQQFVMEKGNCDDSSMS  QRWG+VQAVDAKERTVKVQLR
Sbjct: 301 RGLDSHCLTPANAMNAHEFWPQQFVMEKGNCDDSSMSETQRWGVVQAVDAKERTVKVQLR 360

Query: 361 TNDLSEGSHLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLAMDKFASQSDENNIISEIS 420
           T DL+EGS LDGL VEETMSAYELVEHPDYSF+LGDIVFRLA   F SQSDENNI SEIS
Sbjct: 361 TGDLTEGSDLDGLWVEETMSAYELVEHPDYSFTLGDIVFRLA--SFGSQSDENNITSEIS 420

Query: 421 MNE-SAQNEGNHIINNNNMNMDNFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPID 480
           MNE SAQN  +HII+NN MNMDNFLSFIGNVT FKDG VEVKWANCS+SMVSP EIFPID
Sbjct: 421 MNEESAQNGESHIISNN-MNMDNFLSFIGNVTSFKDGTVEVKWANCSSSMVSPHEIFPID 480

Query: 481 KYEVSSIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAAV 540
           KYEVS+IAVP+G+N+EE R +QIEP KP NIQKGKDMPN D A KNF+RDTTCF SRAA+
Sbjct: 481 KYEVSAIAVPSGDNVEELRQDQIEPSKPDNIQKGKDMPNFDAAGKNFRRDTTCFLSRAAI 540

Query: 541 EFFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEIDLLCEKEVLQTCSQSTEMSQIAKLH 600
           EFFSN+FTTLFGSVRSTI+SGTFTALQSFEGGD ID+L EKEVLQTCSQ T  SQIAKL 
Sbjct: 541 EFFSNVFTTLFGSVRSTIISGTFTALQSFEGGDNIDILPEKEVLQTCSQFTGRSQIAKLK 600

Query: 601 NFGETSLDIEEIHKNEDLTFSTSSKNYDLLTQFDMASDCPDHHFLSSGKGSMLSQASRKW 660
           +FGETSL+ EEIH+NE  T STSS+NYDL TQFDMASDCPDHHFLSSG  SMLSQA R W
Sbjct: 601 SFGETSLNTEEIHENEASTLSTSSENYDLFTQFDMASDCPDHHFLSSGNASMLSQARRGW 660

Query: 661 LKKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQ 720
           LKKVQQEWSILKNNLPETVYVRVFEERMDLIR+VIIGAPGTPYHDGLFFFDI+LPPEYPQ
Sbjct: 661 LKKVQQEWSILKNNLPETVYVRVFEERMDLIRSVIIGAPGTPYHDGLFFFDIFLPPEYPQ 720

Query: 721 IPPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLN 780
           IPPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLN
Sbjct: 721 IPPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLN 780

Query: 781 EKPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFETLVEEHFRRR 840
           EKPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSK PKHFE LVEEHFRRR
Sbjct: 781 EKPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKPPKHFEALVEEHFRRR 840

Query: 841 YQHILAACRAYMEGTPVGSDYQRGTTKPENPNQSSTGFKIMLAKIFPRLIKTFSSKGFDC 900
           YQHILAACRAYMEGTPVG+DY  G  K  NPNQSSTGFKIMLAKI+PRLIKTFSSKG DC
Sbjct: 841 YQHILAACRAYMEGTPVGADYHSGAKKLVNPNQSSTGFKIMLAKIYPRLIKTFSSKGIDC 900

Query: 901 SQFMEA 905
           SQF EA
Sbjct: 901 SQFFEA 903

BLAST of MELO3C007283.jh1 vs. ExPASy TrEMBL
Match: A0A6J1CJP8 (probable ubiquitin-conjugating enzyme E2 24 OS=Momordica charantia OX=3673 GN=LOC111012245 PE=4 SV=1)

HSP 1 Score: 1587 bits (4109), Expect = 0.0
Identity = 786/913 (86.09%), Postives = 831/913 (91.02%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLEEAEY-YHGQTSCILSSLEETIGKIDEFLSFEREFLLG 60
           MDLFLSDSDWENSSECDSSDD EEAEY YHGQTSCILSSLEETIGKIDEFLSFEREF+LG
Sbjct: 1   MDLFLSDSDWENSSECDSSDDPEEAEYFYHGQTSCILSSLEETIGKIDEFLSFEREFMLG 60

Query: 61  DAVCFVNDPYGQMGKVVGIEMLVDLEKNSGPLIKNVNSKDLLKIRSISAGDVVVNGPWIG 120
           D VC VNDP+GQMGKVVGI+MLVDLEKN GP IK+VNSKDLLKIRSIS GDVVV+G WIG
Sbjct: 61  DVVCSVNDPFGQMGKVVGIDMLVDLEKNHGPFIKDVNSKDLLKIRSISVGDVVVHGSWIG 120

Query: 121 RVDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDALDDSQYPYYPGQRVRFSNVSKPPG 180
           RVDKVIDSVSVLFDDG KCEIIAVDQEKLL ISVDALDD QYPYYPGQRVR SNVSK P 
Sbjct: 121 RVDKVIDSVSVLFDDGTKCEIIAVDQEKLLSISVDALDDPQYPYYPGQRVRLSNVSKSPR 180

Query: 181 WLSGWKDDRCEGTVCSVEAGLVYVDWLASILVGCSSSLTAPPRMQHAKELTLLSCFSHAN 240
           WLSGWKDDRCEGTVCSVEAGLVYVDWLAS+L+GCSSSL AP RMQHAK+LTLLSCF HAN
Sbjct: 181 WLSGWKDDRCEGTVCSVEAGLVYVDWLASVLMGCSSSLPAPQRMQHAKKLTLLSCFPHAN 240

Query: 241 WNLGDWCIFPGDEKNNYFGEKFNSTHDLMNGGYRGTGFPEICIIVKKKTKVDVLWQDGSR 300
           W+LGDWCIFP DEK +Y GEKF +THDLMNGGY GTGF EI IIVKKKTKVDVLWQDGSR
Sbjct: 241 WHLGDWCIFPVDEKGDYLGEKFTTTHDLMNGGYCGTGFQEISIIVKKKTKVDVLWQDGSR 300

Query: 301 STALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGIVQAVDAKERTVKVQL 360
           S+ LDS CL PANA NAHEFWPQQ VMEKG C+D S+S+ +RWG+V+AVDAKERTVKVQ 
Sbjct: 301 SSELDSHCLAPANAMNAHEFWPQQLVMEKGTCNDPSISIAERWGVVRAVDAKERTVKVQW 360

Query: 361 RTNDLSEGSHLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLAMDKFASQSDENNIISEI 420
           RT+DL+EG+ LD LLVEETMSAYELVEHPDYSFSLGDIVFRLA+DKF  QSDE+N   EI
Sbjct: 361 RTSDLTEGNDLDILLVEETMSAYELVEHPDYSFSLGDIVFRLALDKFGGQSDEHNTNYEI 420

Query: 421 SMNE-SAQNEGNHIINNNNMNMDNFLSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPI 480
           SM+E SAQN  N +I+N  MNMDNFLSFIGNV GFKDG +EV+WANCSTS V PQEIFPI
Sbjct: 421 SMSEESAQNGENRVIDNT-MNMDNFLSFIGNVIGFKDGVMEVRWANCSTSKVLPQEIFPI 480

Query: 481 DKYEVSSIAVPNGENLEESRLEQIEPLKPYNIQKGKDMPNCDGASKNFKRDTTCFFSRAA 540
           DKYEVS++AVPNGEN+EE R +QIE LKPYNIQKGKDMPNCDGA KNFKRDTTCFFSRAA
Sbjct: 481 DKYEVSTLAVPNGENVEELRQDQIEHLKPYNIQKGKDMPNCDGAGKNFKRDTTCFFSRAA 540

Query: 541 VEFFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEIDLLCEKEVLQTCSQSTEMSQIAKL 600
           +EFFS+IFTTLFGSVRSTILSGTFTALQSFEGGDEI LL EKE LQTC QSTE+SQIAKL
Sbjct: 541 IEFFSSIFTTLFGSVRSTILSGTFTALQSFEGGDEIGLLNEKEELQTCCQSTELSQIAKL 600

Query: 601 HNFGETSLDIEEIHKNEDLTFSTSSKNYDLLTQFDMASDCPDHHFLSSGKGSMLSQASRK 660
            +FGETS  IEE HKNED T STSS+NYDL TQFDMASDCPDHHFLSSG GS+LSQA R 
Sbjct: 601 QDFGETSFKIEENHKNEDFTLSTSSENYDLFTQFDMASDCPDHHFLSSGNGSLLSQARRG 660

Query: 661 WLKKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYP 720
           WLKKVQQEWSILKNNLPET+YVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYP
Sbjct: 661 WLKKVQQEWSILKNNLPETIYVRVFEERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYP 720

Query: 721 QIPPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVL 780
           QIPPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNP SSTILQVLLSLQALVL
Sbjct: 721 QIPPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPVSSTILQVLLSLQALVL 780

Query: 781 NEKPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKSPKHFETLVEEHFRR 840
           NEKPYYNEAGYDAQLGRAEGE+NS SYNENAFLVTCKSMMYLLSK PKHFE LVEEHFRR
Sbjct: 781 NEKPYYNEAGYDAQLGRAEGEKNSDSYNENAFLVTCKSMMYLLSKPPKHFEALVEEHFRR 840

Query: 841 RYQHILAACRAYMEGTPVGSDYQRGTTKPENPNQSSTGFKIMLAKIFPRLIKTFSSKGFD 900
           RYQHILAAC AYMEGTPVGSDY  G TK E PNQSSTGFKIMLAKIFP+L++TFSSKG D
Sbjct: 841 RYQHILAACIAYMEGTPVGSDYHCGATKLEYPNQSSTGFKIMLAKIFPKLVETFSSKGID 900

Query: 901 CSQFMEAENGFIL 911
           CSQF+ AEN  I+
Sbjct: 901 CSQFIRAENVLIV 912

BLAST of MELO3C007283.jh1 vs. TAIR 10
Match: AT2G33770.1 (phosphate 2 )

HSP 1 Score: 842.4 bits (2175), Expect = 3.4e-244
Identity = 443/928 (47.74%), Postives = 606/928 (65.30%), Query Frame = 0

Query: 1   MDLFLSDSDWENSSECDSSDDLEEAEYYHGQTSCILSSLEETIGKIDEFLSFEREFLLGD 60
           M++ L+DSDW++SS+  SS+  E    Y G+   I S+LEETIGKIDEFLSFER F+ GD
Sbjct: 1   MEMSLTDSDWDSSSDSGSSEHEEVEFSYGGRAQNIFSNLEETIGKIDEFLSFERGFMYGD 60

Query: 61  AVCFVNDPYGQMGKVVGIEMLVDLEKNSGPLIKNVNSKDLLKIRSISAGDVVVNGPWIGR 120
            V    +P GQ G+V+ I+M V+LE   G ++K V++K L K+RSIS  D V+NGPW+GR
Sbjct: 61  IVRSATEPSGQSGRVINIDMFVNLESTHGKIMKEVDTKRLQKLRSISLSDYVINGPWVGR 120

Query: 121 VDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDALDDSQYPYYPGQR--VRFSNVSKPP 180
           VDK+++ VSV  DDG   E++   Q+KL+ I  + L+DSQY YYPGQR  V+ ++  +  
Sbjct: 121 VDKIVERVSVTLDDGTNYEVLVDGQDKLVAIPPNLLEDSQYSYYPGQRVQVKLAHAPRST 180

Query: 181 GWLSG-WKDDRCEGTVCSVEAGLVYVDWLASILVGCSSSLTAPPRMQHAKELTLLSCFSH 240
            WL G W+  +  GTVC+VEAGLVYVDW+ASI++    +LTAP  +Q+ + LTLL C SH
Sbjct: 181 TWLCGTWRGTQVMGTVCTVEAGLVYVDWVASIVMEGDRNLTAPQALQNPESLTLLPCVSH 240

Query: 241 ANWNLGDWCIFPGD--------EKNNYFGEKFNSTHDLMNGGY----RGTGFPEICIIVK 300
           A+W LGDWCI PG         +  N      N  H     G+    + +G  E+ +I K
Sbjct: 241 ASWQLGDWCILPGSSHCDIAERQTPNVAAYNLNECHKTFQKGFNRNMQNSGLDELFVITK 300

Query: 301 KKTKVDVLWQDGSRSTALDSPCLTPANATNAHEFWPQQFVMEKGNCDDSSMSVNQRWGIV 360
            K KV V+WQDGS S  +DS  L P  A NAH+FWP+QFV+EK  C+      +++WG+V
Sbjct: 301 TKMKVAVMWQDGSCSLGVDSQQLLPVGAVNAHDFWPEQFVVEKETCN------SKKWGVV 360

Query: 361 QAVDAKERTVKVQLRTNDLSEGSHLDGLLVEETMSAYELVEHPDYSFSLGDIVFRLAMD- 420
           +AV+AKE+TVKVQ       E +     ++EE +SAYEL+EHPD+ F   D+V +L  + 
Sbjct: 361 KAVNAKEQTVKVQWTIQVEKEATGCVDEVMEEIVSAYELLEHPDFGFCFSDVVVKLLPEG 420

Query: 421 KFASQSDENNIISEISMNESAQNEGNHIINNNNMNMDNFLSFIGNVTGFKDGAVEVKWAN 480
           KF   +D   I++          E  H++  ++ +   FLS IG VTGFK+G+V+VKWAN
Sbjct: 421 KFDPNAD--TIVA---------TEAKHLLTESDYSGAYFLSSIGVVTGFKNGSVKVKWAN 480

Query: 481 CSTSMVSPQEIFPIDKYEVSSIAVPNGENLEE---SRLEQIEPLKPYNIQKG--KDMPNC 540
            STS V+P EI+ +++ E S+ +  + E   +    ++ Q +     + + G  K     
Sbjct: 481 GSTSKVAPCEIWKMERSEYSNSSTVSSEGSVQDLSQKISQSDEASSNHQETGLVKLYSVG 540

Query: 541 DGASKNFKRDTTCFFSRAAVEFFSNIFTTLFGSVRSTILSGTFTALQSFEGGDEIDLLCE 600
           +  ++N    ++ F  +AA+ F +N+ ++LFG   ST +  + +     E   + ++L +
Sbjct: 541 ESCNENIPECSSFFLPKAAIGFITNLASSLFGYQGSTSVISSHSRCNDSEDQSDSEVLVQ 600

Query: 601 KEVLQTCSQSTEMSQIAKLHNFGETSLDIEEIHKNEDLTFSTSSKNYDLLTQFDMASDCP 660
           +      +  T   ++          ++ + I+K  D T   +S+N     QFDM +DC 
Sbjct: 601 ETAESYDNSETNSGEVDMTTTMVNIPIEGKGINKTLDSTLLENSRNQVRFRQFDMVNDCS 660

Query: 661 DHHFLSSGKGSMLSQASRKWLKKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPG 720
           DHHFLSS KG   SQ ++ W+KKVQQEWS L+ NLP T+YVRV EERMDL+RA ++GAPG
Sbjct: 661 DHHFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNTIYVRVCEERMDLLRAALVGAPG 720

Query: 721 TPYHDGLFFFDIYLPPEYPQIPPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVW 780
           TPYHDGLFFFDI LPP+YP  PP+VHY SGG+R+NPNLYESG+VCLSLLNTW+GSG+EVW
Sbjct: 721 TPYHDGLFFFDIMLPPQYPHEPPMVHYHSGGMRLNPNLYESGRVCLSLLNTWSGSGTEVW 780

Query: 781 NPGSSTILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMY 840
           N GSS+ILQ+LLS QALVLNEKPY+NEAGYD QLGRAEGE+NS SYNENAFL+TCKSM+ 
Sbjct: 781 NAGSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAEGEKNSVSYNENAFLITCKSMIS 840

Query: 841 LLSKSPKHFETLVEEHFRRRYQHILAACRAYMEGTPVGSDYQRGTTKPENPNQSSTGFKI 900
           +L K PKHFE LV++HF  R QH+LAAC+AYMEG PVGS          N   +STGFKI
Sbjct: 841 MLRKPPKHFEMLVKDHFTHRAQHVLAACKAYMEGVPVGS----SANLQGNSTTNSTGFKI 900

Query: 901 MLAKIFPRLIKTFSSKGFDCSQFMEAEN 908
           ML+K++P+L++ FS  G DC Q +  E+
Sbjct: 901 MLSKLYPKLLEAFSEIGVDCVQEIGPES 907

BLAST of MELO3C007283.jh1 vs. TAIR 10
Match: AT2G16920.1 (ubiquitin-conjugating enzyme 23 )

HSP 1 Score: 602.8 bits (1553), Expect = 4.5e-172
Identity = 364/984 (36.99%), Postives = 530/984 (53.86%), Query Frame = 0

Query: 41   ETIGKIDEFLSFEREFLLGDAVCFVNDPYGQMGKVVGIEMLVDLEKNSGPLIKNVNSKDL 100
            E +  I++    +R FL GD V   ++P GQ+G VV + + VDL    G + K++++K+L
Sbjct: 130  EPVQDINDVTVIDRGFLHGDYVASASEPTGQVGVVVDVNISVDLLAPDGSIHKDISTKNL 189

Query: 101  LKIRSISAGDVVVNGPWIGRVDKVIDSVSVLFDDGNKCEIIAVDQEKLLPISVDAL-DDS 160
             ++R  + GD VV+GPW+GR+D V+D+V+VLFDDG+ C+++ V+  +L PI  + L +D+
Sbjct: 190  KRVRDFAVGDYVVHGPWLGRIDDVLDNVTVLFDDGSMCKVLRVEPLRLKPIPKNNLEEDA 249

Query: 161  QYPYYPGQRVR--FSNVSKPPGWLSG-WKDDRCEGTVCSVEAGLVYVDWLASILVGCSSS 220
             +PYYPGQRV+   S+V K   WLSG WK +R EGTV  V AG ++V W+AS   G  SS
Sbjct: 250  NFPYYPGQRVKASSSSVFKNSRWLSGLWKPNRLEGTVTKVTAGSIFVYWIASAGFGPDSS 309

Query: 221  LTAPPRMQHAKELTLLSCFSHANWNLGDWCIFPG----------------------DEKN 280
            + +PP  Q+   LTLLSCF+HANW +GDWC+ P                        ++ 
Sbjct: 310  V-SPPEEQNPSNLTLLSCFTHANWQVGDWCLLPSLNQSATIPLHKHVSKLRLYDSQADRQ 369

Query: 281  NYFGEKFNSTHDLMNGGYRGTG-------------------------------------- 340
               G       D ++G     G                                      
Sbjct: 370  QKIGRDLEDVQDEVSGKVEPAGITAEALPKVTSDDPPQRNPSVSKEPVHEPWPLHRKKIR 429

Query: 341  ----------------FPEICIIVKKKTKVDVLWQDGSRSTALDSPCLTPANATNAHEFW 400
                            F +  ++V  +T+VDV WQDG+     ++  L P      HEF 
Sbjct: 430  KLVIRKDKKVKKKEESFEQALLVVNSRTRVDVSWQDGTIECRREAITLIPIETPGDHEFV 489

Query: 401  PQQFVMEKGNCDDSSMSVNQRWGIVQAVDAKERTVKVQLRTNDLSEGSHLDGLLVEETMS 460
             +Q+V+EK + D  + +  +R G+V+ V+AK+RT  V+   N L           EE +S
Sbjct: 490  SEQYVVEKTSDDGDNTTEPRRAGVVKNVNAKDRTASVRW-LNPLRRAEEPREFEKEEIVS 549

Query: 461  AYELVEHPDYSFSLGDIVFRLAMDKFA--SQSDENNIISEISMNESAQNEGNH----IIN 520
             YEL  HPDY +  GD+V RL+    A  + S  N+       +   Q+  +H    I+ 
Sbjct: 550  VYELEGHPDYDYCYGDVVVRLSPIAVALPASSPGNSFEEATQQDNGYQDSESHQEAKILV 609

Query: 521  NNNMNMDNF----LSFIGNVTGFKDGAVEVKWANCSTSMVSPQEIFPIDKYEVSSIAVPN 580
            +   N  +     LS++GN+TG KDG +EV WA+ + S V P  ++ + + +        
Sbjct: 610  DKEENEPSTDLSKLSWVGNITGLKDGDIEVTWADGTISTVGPHAVYVVGRDDDDESVAGE 669

Query: 581  GENLEESRLEQIE-------PLKPYNIQKGKDMP-NCDG---ASKNFKRDTTCFFSRAAV 640
             E  + +  E +         +   ++ +   +  N D    A  +  R+       AA+
Sbjct: 670  SETSDAASWETLNDDDRGAPEIPEEDLGRSSSIEGNSDADIYAENDSGRNGALALPLAAI 729

Query: 641  EFFSNIFTTLFGSVRSTI--------LSGTFTALQSFEGGDEIDLL-----CEKEVLQTC 700
            EF + + + +F   R ++        +   +   +S    DE D L      +  V   C
Sbjct: 730  EFVTRLASGIFSRARKSVDSSSSDYTVENVYKQAESTNPSDETDSLDDPSPSKVNVTDNC 789

Query: 701  -SQSTEMSQIAKLHNFGETSLDIEEIHKNEDLTFSTSSKNYDLLTQFDMASDCPDHHFLS 760
             S+ T+ +  AK    GETS  +E+    ED    +   +     +FD++ D  DHHFL 
Sbjct: 790  ESKGTQAN--AKNILSGETSTFLED----EDKPVPSEGDSCS-FRRFDISQDPLDHHFLG 849

Query: 761  -SGKGSMLSQASRKWLKKVQQEWSILKNNLPETVYVRVFEERMDLIRAVIIGAPGTPYHD 820
              G+        R+W KKV Q+W IL+NNLP+ ++VR +E+RMDL+RAVI+GA GTPY D
Sbjct: 850  VDGQ----KTKERQWFKKVDQDWKILQNNLPDGIFVRAYEDRMDLLRAVIVGAFGTPYQD 909

Query: 821  GLFFFDIYLPPEYPQIPPLVHYISGGLRVNPNLYESGKVCLSLLNTWTGSGSEVWNPGSS 880
            GLFFFD +LP +YP +PP  +Y SGG R+NPNLYE GKVCLSLLNTWTG G+EVW+P SS
Sbjct: 910  GLFFFDFHLPSDYPSVPPSAYYHSGGWRLNPNLYEEGKVCLSLLNTWTGRGNEVWDPKSS 969

Query: 881  TILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSASYNENAFLVTCKSMMYLLSKS 907
            +ILQVL+SLQ LVLN KPY+NEAGYD Q+G AEGE+NS  YNEN FL+ CK+MMYL+ K 
Sbjct: 970  SILQVLVSLQGLVLNSKPYFNEAGYDKQVGTAEGEKNSLGYNENTFLLNCKTMMYLMRKP 1029

BLAST of MELO3C007283.jh1 vs. TAIR 10
Match: AT1G53025.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 288.5 bits (737), Expect = 1.9e-77
Identity = 142/267 (53.18%), Postives = 175/267 (65.54%), Query Frame = 0

Query: 631 QFDMASDCPDHHFLSSGKGSMLSQASRKWLKKVQQEWSILKNNLPETVYVRVFEERMDLI 690
           +FD   D  DHH+ S GK S   Q S+ W+KKVQ +W IL+N+LPE + VR  E RMDL+
Sbjct: 246 RFDTVEDFSDHHYASKGKSS--KQHSKNWVKKVQADWKILENDLPEAISVRACESRMDLL 305

Query: 691 RAVIIGAPGTPYHDGLFFFDIYLPPEYPQIPPLVHYISGGLRVNPNLYESGKVCLSLLNT 750
           RAVIIGA GTPYHDGLFFFDI  P  YP +PP VHY SGGLR+NPNLY  GKVCLSLL T
Sbjct: 306 RAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVHYHSGGLRINPNLYNCGKVCLSLLGT 365

Query: 751 WTGSGSEVWNPGSSTILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSASYNENAF 810
           W GS  E W P  ST+LQ+L+S+QAL+LNEKPY+NE GY    G A GE  S  Y+EN F
Sbjct: 366 WAGSAREKWLPNESTMLQLLVSIQALILNEKPYFNEPGYVQSAGTASGESKSKVYSENVF 425

Query: 811 LVTCKSMMYLLSKSPKHFETLVEEHFRRRYQHILAACRAYMEGTPVGSDYQRGTTKPENP 870
           L++ K+M+Y + + P+HFE  V+ H+  R   I+ AC AY  G P+GS  + G    E  
Sbjct: 426 LLSLKTMVYSIRRPPQHFEEYVQNHYFVRSHDIVKACNAYKAGAPLGSMVKGGVQDLEEA 485

Query: 871 NQS-STGFKIMLAKIFPRLIKTFSSKG 897
            QS S  FK  +A     ++  F   G
Sbjct: 486 RQSGSKKFKTDVASFMQTVVDEFVKLG 510

BLAST of MELO3C007283.jh1 vs. TAIR 10
Match: AT3G15355.1 (ubiquitin-conjugating enzyme 25 )

HSP 1 Score: 272.3 bits (695), Expect = 1.4e-72
Identity = 124/232 (53.45%), Postives = 161/232 (69.40%), Query Frame = 0

Query: 626 YDLLTQFDMASDCPDHHFLSSGKGSMLSQASRKWLKKVQQEWSILKNNLPETVYVRVFEE 685
           Y    +FD+  D  DHH+ S  KG+   Q S+ W K++Q EW IL+ +LPE ++VR +E 
Sbjct: 302 YLFFKRFDIVEDFSDHHYAS--KGTTSKQHSKDWAKRIQDEWRILEKDLPEMIFVRAYES 361

Query: 686 RMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQIPPLVHYISGGLRVNPNLYESGKVCL 745
           RMDL+RAVIIGA GTPYHDGLFFFDI+ P  YP  PP+VHY SGGLR+NPNLY  GKVCL
Sbjct: 362 RMDLLRAVIIGAQGTPYHDGLFFFDIFFPDTYPSTPPIVHYHSGGLRINPNLYNCGKVCL 421

Query: 746 SLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSASY 805
           SLL TW+G+  E W P +ST+LQVL+S+Q L+LN+KPY+NE GY+   G A GE  S +Y
Sbjct: 422 SLLGTWSGNQREKWIPNTSTMLQVLVSIQGLILNQKPYFNEPGYERSAGSAHGESTSKAY 481

Query: 806 NENAFLVTCKSMMYLLSKSPKHFETLVEEHFRRRYQHILAACRAYMEGTPVG 858
           +EN F+++ K+M+Y + + PK+FE     HF      +L AC AY  G   G
Sbjct: 482 SENTFILSLKTMVYTMRRPPKYFEDFAYGHFFSCAHDVLKACNAYRNGATPG 531

BLAST of MELO3C007283.jh1 vs. TAIR 10
Match: AT1G53023.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 268.9 bits (686), Expect = 1.5e-71
Identity = 133/273 (48.72%), Postives = 171/273 (62.64%), Query Frame = 0

Query: 631 QFDMASDCPDHHFLSSGKGSMLS-------QASRKWLKKVQQEWSILKNNLPETVYVRVF 690
           +FD   D  DHH+ + G  + LS          + W+K +Q+EW IL  NLPET++VR  
Sbjct: 23  RFDTVEDFSDHHYAAEGSPAKLSLWHLKFKGRPKNWVKDIQKEWKILDKNLPETIFVRAC 82

Query: 691 EERMDLIRAVIIGAPGTPYHDGLFFFDIYLPPEYPQIPPLVHYISGGLRVNPNLYESGKV 750
           E R+DL+RAVIIGA GTPYHDGLFFFDI  P  YP +PP VHY SGGLR+NPNLY+ GKV
Sbjct: 83  ESRIDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPKVHYHSGGLRINPNLYKCGKV 142

Query: 751 CLSLLNTWTGSGSEVWNPGSSTILQVLLSLQALVLNEKPYYNEAGYDAQLGRAEGERNSA 810
           CLSL++TWTG   E W P  ST+LQ+L+S+QAL+LNEKPYYNE GY+  +G   GE  S 
Sbjct: 143 CLSLISTWTGKKREKWLPKESTMLQLLVSIQALILNEKPYYNEPGYEKSMGTPLGESYSK 202

Query: 811 SYNENAFLVTCKSMMYLLSKSPKHFETLVEEHFRRRYQHILAACRAYMEGTPVGSDYQRG 870
            Y+EN F+ + K+M         HFE  V  HF  R   I+ AC AY +G PVGS   +G
Sbjct: 203 DYSENVFVFSLKTM---------HFEEFVRSHFFVRSHDIVKACNAYKDGAPVGS-IDKG 262

Query: 871 TTKPENPNQSSTGFKIMLAKIFPRLIKTFSSKG 897
             K +   + S  F+I +      ++  F + G
Sbjct: 263 GVKKQTRQRGSLKFRINVTSFMKTVVDEFVNLG 285