MELO3C007158 (gene) Melon (DHL92) v4

Overview
NameMELO3C007158
Typegene
OrganismCucumis melo cv. DHL92 (Melon (DHL92) v4)
Descriptiontwo-pore potassium channel 3
Locationchr08: 1160920 .. 1165533 (-)
RNA-Seq ExpressionMELO3C007158
SyntenyMELO3C007158
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAAAGGAGAAGGAAGAAGAAATTCGATTCCCAATCACAGATTACATAACGTTGTCTTCACAGGTTGGGTTGGGCGATCCGTTGTCAATGATCAATCATCTGCCCTTTGCAAATGGCGCGTGAAATGGATTTCTTCTTCTTCCTCTTTATCCGATCCCTTCTTCTTCGTCTTCCTCTGTTTTTTCTTTTTCTTTTCTGTTTACTGCTCGCTGTAATGTAATTATCCAACTGGGTTTCCTCCATTTTTCACCATATTCAAACCTTTCAGTTGCTGGGTCCCAACAAAATACTTCTATCTTCCTTATTAACTCCCATTTGAGCTCTCACTTTCTTCATTTGACTTGGAATTCATGCCTCAAAATCCCTCCCCTGCCTTGGTTTCATTTTGACGTCTTCTGTTTCTGCAAGCCTGCTGGTTGTAGTTTGTCCTCACAGGTCAACCATCCCCGTATCTCAATGGAGAAAGAGCCTCTTCTTCCATATCTAAGCCCAAGAGGGAAACCGCCTCCGCCACCTCCTCAATTATGTCCACTCCCTGAAAACGATGAAATTACTCTGCCTATGACACCCACGGAGTTCAAAGACCGCTTGATCTTTGGTCCTTCATCGTCCCCACAAGACGCTTCTCCTATATTCGACGCTTTGACGCTATCTCTTTCCTCTTCGAGACCCTCTGCTTCATCTTCTCTTCAAGACCCATCTACACCTTTGGACCCAAGATCACAATCGCAGTCTCAGCAGGCGTGGCTTGTGGATTCCAATTACACATGGCCCAAAAGCAACCTCCATCGCTCCAAAACTGCACCTGCCATGGCGGTCATCAACGATGTCAATCATTCGCAGGAGCCCAAGCCACAATTTGGTAAACAGTCTATTATCCGCCAGGCGGTTGTTCTTCTAATTGTTTACTTATCATTGGGTGTGCTTATCTATTGGTTAAATCGTGATAATTTTGCGGCTCAAGAGACTCATCCTGTGGTCGATGCATTGTACTTTTGTATTGTAACGATGTGCACTATTGGATATGGAGATATTACCCCCAATAGTACAGCGACAAAGCTGTTCTCAGTATTGTTTGTGTTGGTGGGATTTGGGTTTATAGATATATTGCTCTCTGGAATGGTTAGTTATGTGCTCGATTTGCAGGAGAGTTATTTGTTGAGGAATGTAAAGAGGGGGGTGAAGCGTGAATCTGGGAAGTCCTATATAATTGATGTGAAAAAGGGAAGAATGAGGATTAGAATGAAGGTGGCATTGGCCTTGGGTGTTGTGATACTTTGTATAGGAGTTGGCATGGGTGTTATGCATTTTGTTGAGAACCTGGGATGGTTGGATTCATTTTATCTCTCCGTTATGTCCGTCACAACAGTTGGATATGGTGATCAGGCATTCAAGTCGATGACTGGTCGGATTTTTGCTTCAATCTGGTTGCTTGTATCAACCCTTGCTGTTGCTCGGGCGTTTCTTTATTTAGCGGAGGCAAGAGTAGACAAGCGGCATAGGATGATGGCAAAATGGATACTTGGTCAAGATATGACCATTTCTGAGTTTCTTGCTGCTGATATTGACAACAATGGCTTTGTGAGGTATGCATTAACTTTATTCATTCACTCATTTAGAGAAGTTGCTTGGAAAATTGGATTTGATGTTACTGATTCTGATTGCCGAGAAGTTCTGCTTGCATTGTTTCATGAAAATGCATGAGTATTTGTTGCATAAGTTTTATGAAACTGCTCATATATTCTGGTTTGTTTGAAGAGCGAGCCATTGGCTGAAAAATTTACGTTTTAAATGTGCATTCGTGGTATCTCTCTTATGAATATGATCATATTAATAATTCTGTGAGACTTTCTACTTTTACAGCATCCAACTCAGCCTTCTATTTGGCATAGTTACTGCTAAAGTTTGATATTATTTATCAACACAGTCTTCCCTCTCCCTAGTACAGCGAACTAACATATACAAAAAAGGATACCCCCCCTCCGACTTCGTCTTTCTCCCTTTTGGCATCCAGTATCCCAACTCATGAGTCATGCCCATTATGCAATCTCAAAATCCTGTTGACAGTAGATTCTTCAAAATTATTACATTCAACCCAACTCCCTCTCTGAGACGGTACCATTTTGTAATAGAGGGTCTATCAATAAAGCATGCTGATTATATGAATCTTTCAGATAGAAAGAAAAGAGTTTTTTTCTTTCTTTCTTTTTTTTTTTTTTTATGAAAGGGAAGAGACTTCGGACTTGGTTCTGTTGAAGGCTTCTACTTGGTGTATCAATTACACTTTTGCAATAATGATTCTCTTGTTTTTAACTCTTAGTCAACTGCTGAGTTTTTTTCTTGTAAACTCCATGAACCTCTTTGCTTCCTTTTGACATCATCATTGTAAAGTTCAGTTTTGTATTAGAAAATCAAAGAAGAACCTTTAGATGACAATTTCGCTGTTTCTGAAGCAATTGTTGCAATTTTGTTTGGATGGAATGAGTTTTTAGTTCTGTTGGTTATTTCATGGATGAATTAAAAGCAAGAGAATGAAGTTTCAGCTTTAACTTTTGAGAAGTTTGTGGAAAGCTAATTTGTAATAAGAGGAGAGAAATAAAGGATATGGTTTTTCAATATTCAAAATGAAGTGCGACACTATTGCTGTTGGTTTTTGTTTGATTCTGATATGAAAATAATCAAAACTCGTGTACATGTAGTGAGGGTGTCTTCAAAGACTATAGATTTTGCAGAGGAAAGTGTTGGGATCCCAGTAGGGAAATATTAAAAATTATTCCTTAGTAATTAGTTGGTAAATTTGTTAGAAGTGAATGGTTATAGATGGAGTTATGGAACAGAATGTAGGCAAATTTTGAGAGAATGTAGATGTCTCGAAGTGCTTAGAACGTCATAAATTTTTCTAACGTTAATAGCATATTTCCGTTAGTTCTACTCCCGGAAGAAGTACTATACAATTATTGTTTAGTCATGGGAAGATATTGCACAAATAAAAATTAGAAAAGCCTAGAAGATTGTGAAAGCCTCCTCCTCAAACTATTCTAAATAGAAATCACCTCAGTCCAAAGCCCGCATTTCATCAGTATAGTAGGAGAGGAAAGAATAATAAGGGCCAGGGAATTGTAAGGGGTGAGGACCACTTGGGAGAATAGTATAAATATAGTCAAAGTGCATTGAATTAGTTAATTAGGAATTGAAGAGTATCTTCTCGAGAGTGAGAATAGGGAGTTAGGGACGATCGCCTGTGTCCCTAGTTATTCTACTGTGATTTTGGTGTACAGTCCATTACTATCACAATAAAGATCGATACTTTTCAGAATGTGGGTACAGAAGTTTCACAATTGAATGAAATTTGTGAGTGTCGTTTTTTTCCTTGTAAAGGTCCTGGCTTCTGAATAGTAATTAGTTCTTCATTCGATAAAATTTGAAAAAGTGGCAACTGAGAAATCATGATATATTTCTTTGCAATTAGTCTTCTGGTAGGACCCCTTTTTCTTTTAACAAGAAACGATTTATTGTAATAATGACGAGAAATGGGTTTCTTCTGTACATGCTTTCAGTGATCATGTTGTTTGTTGTGCACTCGGGCTAAACCTGTTGGAATCGTATTTCATGTTTCGATTACGATTACCATAGTAATTATGGTGGTGGATTCACTGAAGTGAACAAGACATAAGATCAACCATTTTATATTTTCTGGTTTTACTCTGATTGAAACTCTACAAGATGATCTTGATCATCCAAAATTATAATTGATTCTTTTTTGAACGTGTTTTTTGTTCAATCTGTAATTCCATCTCTAGTTTGCTTTCTAGTACCAGTTTGAGTTCCGGCTTTCCTTCCCAATCATCTTATCCTGTTCAATTCCCCCAATTCTTCTGCCCTCTTAAATATCCTAGTCAAGCAGGTTTGGCTGGTATATCAATCGACATGTAGCTCATTTGGTACGCATATCATGTTCTTAATCGAGTAATATAATTAATTCCCCTTGCCTTCTGATAGTTTCCATTTTACTAGTTTTGCAAGCTCTCTTGTGGATGAACAGTCTTCCCATTTAGTTTGACATCAAACAGAACATTAAAACTTGGTGAGCTCACTTTGTATATTTTATGTTATCCCTTTTGCAGTAGGTCAGAATATGTGATATACAAGCTGAAGGAGATGGGTAGGGTATCCGAGAAAGATGTCATGCAGATATCCAATAACTTCGATAGGCTAGACAGTGGCAAATGTGGGAAGATTACACTGGCTGATCTCTTGGAAAGTCATCACTAATGTGAAAATGCGATACTGTCAACGAAGAACTCGAGTTATTGGTCGTCACAAATTGAAAGCAATTTCATTTTCAAATTGCAGATATATAGTTGAAAGTGCATATATCTGGGACGTGCAAAGCAAGACCTGAAAGAGAGTTTGCATTTCCCAGAACAATTATTTGCTGCTCTTTCACCGATGATTCTGTACAAATTTTGATAGCATAGGAAGTATTCTTCTATAGAAACTAAAAGATAATTGTTTGAACTTGTTGGACTGTAATTTGTTTATTGGTTTATACCTTTGTATGTGAGCATGGGATGTTGCTCTTGAATATAAATGTCCATAGCTGCCTTTATCCTT

mRNA sequence

AGAAAGGAGAAGGAAGAAGAAATTCGATTCCCAATCACAGATTACATAACGTTGTCTTCACAGGTTGGGTTGGGCGATCCGTTGTCAATGATCAATCATCTGCCCTTTGCAAATGGCGCGTGAAATGGATTTCTTCTTCTTCCTCTTTATCCGATCCCTTCTTCTTCGTCTTCCTCTGTTTTTTCTTTTTCTTTTCTGTTTACTGCTCGCTGTAATGTAATTATCCAACTGGGTTTCCTCCATTTTTCACCATATTCAAACCTTTCAGTTGCTGGGTCCCAACAAAATACTTCTATCTTCCTTATTAACTCCCATTTGAGCTCTCACTTTCTTCATTTGACTTGGAATTCATGCCTCAAAATCCCTCCCCTGCCTTGGTTTCATTTTGACGTCTTCTGTTTCTGCAAGCCTGCTGGTTGTAGTTTGTCCTCACAGGTCAACCATCCCCGTATCTCAATGGAGAAAGAGCCTCTTCTTCCATATCTAAGCCCAAGAGGGAAACCGCCTCCGCCACCTCCTCAATTATGTCCACTCCCTGAAAACGATGAAATTACTCTGCCTATGACACCCACGGAGTTCAAAGACCGCTTGATCTTTGGTCCTTCATCGTCCCCACAAGACGCTTCTCCTATATTCGACGCTTTGACGCTATCTCTTTCCTCTTCGAGACCCTCTGCTTCATCTTCTCTTCAAGACCCATCTACACCTTTGGACCCAAGATCACAATCGCAGTCTCAGCAGGCGTGGCTTGTGGATTCCAATTACACATGGCCCAAAAGCAACCTCCATCGCTCCAAAACTGCACCTGCCATGGCGGTCATCAACGATGTCAATCATTCGCAGGAGCCCAAGCCACAATTTGGTAAACAGTCTATTATCCGCCAGGCGGTTGTTCTTCTAATTGTTTACTTATCATTGGGTGTGCTTATCTATTGGTTAAATCGTGATAATTTTGCGGCTCAAGAGACTCATCCTGTGGTCGATGCATTGTACTTTTGTATTGTAACGATGTGCACTATTGGATATGGAGATATTACCCCCAATAGTACAGCGACAAAGCTGTTCTCAGTATTGTTTGTGTTGGTGGGATTTGGGTTTATAGATATATTGCTCTCTGGAATGGTTAGTTATGTGCTCGATTTGCAGGAGAGTTATTTGTTGAGGAATGTAAAGAGGGGGGTGAAGCGTGAATCTGGGAAGTCCTATATAATTGATGTGAAAAAGGGAAGAATGAGGATTAGAATGAAGGTGGCATTGGCCTTGGGTGTTGTGATACTTTGTATAGGAGTTGGCATGGGTGTTATGCATTTTGTTGAGAACCTGGGATGGTTGGATTCATTTTATCTCTCCGTTATGTCCGTCACAACAGTTGGATATGGTGATCAGGCATTCAAGTCGATGACTGGTCGGATTTTTGCTTCAATCTGGTTGCTTGTATCAACCCTTGCTGTTGCTCGGGCGTTTCTTTATTTAGCGGAGGCAAGAGTAGACAAGCGGCATAGGATGATGGCAAAATGGATACTTGGTCAAGATATGACCATTTCTGAGTTTCTTGCTGCTGATATTGACAACAATGGCTTTGTGAGTAGGTCAGAATATGTGATATACAAGCTGAAGGAGATGGGTAGGGTATCCGAGAAAGATGTCATGCAGATATCCAATAACTTCGATAGGCTAGACAGTGGCAAATGTGGGAAGATTACACTGGCTGATCTCTTGGAAAGTCATCACTAATGTGAAAATGCGATACTGTCAACGAAGAACTCGAGTTATTGGTCGTCACAAATTGAAAGCAATTTCATTTTCAAATTGCAGATATATAGTTGAAAGTGCATATATCTGGGACGTGCAAAGCAAGACCTGAAAGAGAGTTTGCATTTCCCAGAACAATTATTTGCTGCTCTTTCACCGATGATTCTGTACAAATTTTGATAGCATAGGAAGTATTCTTCTATAGAAACTAAAAGATAATTGTTTGAACTTGTTGGACTGTAATTTGTTTATTGGTTTATACCTTTGTATGTGAGCATGGGATGTTGCTCTTGAATATAAATGTCCATAGCTGCCTTTATCCTT

Coding sequence (CDS)

ATGGAGAAAGAGCCTCTTCTTCCATATCTAAGCCCAAGAGGGAAACCGCCTCCGCCACCTCCTCAATTATGTCCACTCCCTGAAAACGATGAAATTACTCTGCCTATGACACCCACGGAGTTCAAAGACCGCTTGATCTTTGGTCCTTCATCGTCCCCACAAGACGCTTCTCCTATATTCGACGCTTTGACGCTATCTCTTTCCTCTTCGAGACCCTCTGCTTCATCTTCTCTTCAAGACCCATCTACACCTTTGGACCCAAGATCACAATCGCAGTCTCAGCAGGCGTGGCTTGTGGATTCCAATTACACATGGCCCAAAAGCAACCTCCATCGCTCCAAAACTGCACCTGCCATGGCGGTCATCAACGATGTCAATCATTCGCAGGAGCCCAAGCCACAATTTGGTAAACAGTCTATTATCCGCCAGGCGGTTGTTCTTCTAATTGTTTACTTATCATTGGGTGTGCTTATCTATTGGTTAAATCGTGATAATTTTGCGGCTCAAGAGACTCATCCTGTGGTCGATGCATTGTACTTTTGTATTGTAACGATGTGCACTATTGGATATGGAGATATTACCCCCAATAGTACAGCGACAAAGCTGTTCTCAGTATTGTTTGTGTTGGTGGGATTTGGGTTTATAGATATATTGCTCTCTGGAATGGTTAGTTATGTGCTCGATTTGCAGGAGAGTTATTTGTTGAGGAATGTAAAGAGGGGGGTGAAGCGTGAATCTGGGAAGTCCTATATAATTGATGTGAAAAAGGGAAGAATGAGGATTAGAATGAAGGTGGCATTGGCCTTGGGTGTTGTGATACTTTGTATAGGAGTTGGCATGGGTGTTATGCATTTTGTTGAGAACCTGGGATGGTTGGATTCATTTTATCTCTCCGTTATGTCCGTCACAACAGTTGGATATGGTGATCAGGCATTCAAGTCGATGACTGGTCGGATTTTTGCTTCAATCTGGTTGCTTGTATCAACCCTTGCTGTTGCTCGGGCGTTTCTTTATTTAGCGGAGGCAAGAGTAGACAAGCGGCATAGGATGATGGCAAAATGGATACTTGGTCAAGATATGACCATTTCTGAGTTTCTTGCTGCTGATATTGACAACAATGGCTTTGTGAGTAGGTCAGAATATGTGATATACAAGCTGAAGGAGATGGGTAGGGTATCCGAGAAAGATGTCATGCAGATATCCAATAACTTCGATAGGCTAGACAGTGGCAAATGTGGGAAGATTACACTGGCTGATCTCTTGGAAAGTCATCACTAA

Protein sequence

MEKEPLLPYLSPRGKPPPPPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIFDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMAVINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYFCIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKRGVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDMTISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGKITLADLLESHH
Homology
BLAST of MELO3C007158 vs. NCBI nr
Match: XP_008439665.1 (PREDICTED: two-pore potassium channel 3 [Cucumis melo])

HSP 1 Score: 826.6 bits (2134), Expect = 9.6e-236
Identity = 425/425 (100.00%), Postives = 425/425 (100.00%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPPPPPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60
           MEKEPLLPYLSPRGKPPPPPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF
Sbjct: 1   MEKEPLLPYLSPRGKPPPPPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120
           DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA
Sbjct: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYF 180
           VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYF
Sbjct: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300
           GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM
Sbjct: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGKITLADL 420
           TISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGKITLADL
Sbjct: 361 TISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGKITLADL 420

Query: 421 LESHH 426
           LESHH
Sbjct: 421 LESHH 425

BLAST of MELO3C007158 vs. NCBI nr
Match: XP_004134597.1 (two-pore potassium channel 3 [Cucumis sativus])

HSP 1 Score: 814.7 bits (2103), Expect = 3.8e-232
Identity = 418/425 (98.35%), Postives = 423/425 (99.53%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPPPPPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60
           MEKEPLLPYLSPRGKP P PPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF
Sbjct: 1   MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120
           DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRS+TAPAMA
Sbjct: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSRTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYF 180
           VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNF+AQETHPVVDALYF
Sbjct: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNST+TKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300
           GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM
Sbjct: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGKITLADL 420
           T+SEFLAADIDNNGFVSRSEYVIYKLKEMG+VSEKDVMQISNNFDRLDSGKCGKITLADL
Sbjct: 361 TVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCGKITLADL 420

Query: 421 LESHH 426
           LESHH
Sbjct: 421 LESHH 425

BLAST of MELO3C007158 vs. NCBI nr
Match: XP_038883350.1 (two-pore potassium channel 3-like [Benincasa hispida])

HSP 1 Score: 801.6 bits (2069), Expect = 3.3e-228
Identity = 410/425 (96.47%), Postives = 419/425 (98.59%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPPPPPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60
           MEKEPLLPY+SPRGKPPPPP QLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF
Sbjct: 1   MEKEPLLPYVSPRGKPPPPPAQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120
           DALTLSLSSSRPSASSSLQDPSTPLD +SQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA
Sbjct: 61  DALTLSLSSSRPSASSSLQDPSTPLDLKSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYF 180
           VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGV IYWLNRD F+A ETHPVVDALYF
Sbjct: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVFIYWLNRDKFSAHETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSY+LDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYMLDLQESYLLRNVKR 240

Query: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300
           G++RESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGM VMHFVENLGWLDSFYLSVM
Sbjct: 241 GLQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMSVMHFVENLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGKITLADL 420
           T+SEFLAADIDNNGFVSRSEYVIYKLKEMG+VSEKDVMQISNNFDRLD+GKCGKITLADL
Sbjct: 361 TVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDTGKCGKITLADL 420

Query: 421 LESHH 426
           LESHH
Sbjct: 421 LESHH 425

BLAST of MELO3C007158 vs. NCBI nr
Match: XP_023544618.1 (two-pore potassium channel 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 781.9 bits (2018), Expect = 2.7e-222
Identity = 402/426 (94.37%), Postives = 414/426 (97.18%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPPPPPPQ-LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPI 60
           MEKEPLLPY+SPRGKP PPPPQ LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQD SPI
Sbjct: 1   MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAM 120
           FDALTLSLSSSRPSASSSLQDPSTPL P+SQS SQQAWLVDSNY+W KSNLHRSKTAPAM
Sbjct: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSNYSWHKSNLHRSKTAPAM 120

Query: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALY 180
           AVINDVNHSQEPKPQFGKQSIIRQAVVLLI+YLSLGVLIYW+NRD F+A ETHPVVDALY
Sbjct: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240
           FCIVTMCTIGYGDITPNS ATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK
Sbjct: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSV 300
           RGV+RESGKSYIIDVKKGRMRIRMKVALALGVVILCIG+GMGVMHFVENLGWLDSFYLSV
Sbjct: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGLGMGVMHFVENLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360
           MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGKITLAD 420
           MT+SEFLAAD DNNG VSRSEYV+YKLKEMG+VSEKDVMQISNNFDRLD+G CGKITLAD
Sbjct: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420

Query: 421 LLESHH 426
           LLESHH
Sbjct: 421 LLESHH 426

BLAST of MELO3C007158 vs. NCBI nr
Match: XP_022926002.1 (two-pore potassium channel 3-like [Cucurbita moschata])

HSP 1 Score: 781.9 bits (2018), Expect = 2.7e-222
Identity = 402/426 (94.37%), Postives = 414/426 (97.18%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPPPPPPQ-LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPI 60
           MEKEPLLPY+SPRGKP PPPPQ LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQD SPI
Sbjct: 1   MEKEPLLPYISPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAM 120
           FDALTLSLSSSRPSASSSLQDPSTPL P+SQS SQQAWLVDSNY+W KSNLHRSKTAPAM
Sbjct: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSNYSWHKSNLHRSKTAPAM 120

Query: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALY 180
           AVINDVNHSQEPKPQFGKQSIIRQAVVLLI+YLSLGVLIYW+NRD F+A ETHPVVDALY
Sbjct: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240
           FCIVTMCTIGYGDITPNS ATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK
Sbjct: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSV 300
           RGV+RESGKSYIIDVKKGRMRIRMKVALALGVVILCIG+GMGVMHFVENLGWLDSFYLSV
Sbjct: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGLGMGVMHFVENLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360
           MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGKITLAD 420
           MT+SEFLAAD DNNG VSRSEYV+YKLKEMG+VSEKDVMQISNNFDRLD+G CGKITLAD
Sbjct: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420

Query: 421 LLESHH 426
           LLESHH
Sbjct: 421 LLESHH 426

BLAST of MELO3C007158 vs. ExPASy Swiss-Prot
Match: Q9SVV6 (Two-pore potassium channel 3 OS=Arabidopsis thaliana OX=3702 GN=TPK3 PE=2 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 3.2e-141
Identity = 279/428 (65.19%), Postives = 340/428 (79.44%), Query Frame = 0

Query: 4   EPLLPY-LSPRGKPPPPPPQLCPLPENDE--ITLPMTPTEFKDRLIFGP-SSSPQDASPI 63
           +PLL Y +SPR K   PP  L PLPE++E  I +PMTP+EFK+RLIFGP S SP+D+S  
Sbjct: 7   DPLLQYMISPRLK--KPPQLLFPLPEDNEVAIPMPMTPSEFKERLIFGPFSCSPRDSSHF 66

Query: 64  FDALTL-SLSSSRPSASSSLQDPST--PLDPRSQSQSQQAWLVDSNYTWPKSN-LHRSKT 123
            D++   S SSS  + ++   D ST  PL P    Q  + WL D   +  + + LHRSKT
Sbjct: 67  IDSMKQPSPSSSSTAVNNPFSDSSTLDPLLPPPPPQ-PEPWLSDQTSSHCQGHALHRSKT 126

Query: 124 APAMAVINDVNHS-QEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPV 183
           APAMAVIND++H  ++  P    +S++RQA  LL+VYLSLGVLIYWLNRD++   +THPV
Sbjct: 127 APAMAVINDLHHPIRQKDPTETSRSVVRQAFALLVVYLSLGVLIYWLNRDHYVVNQTHPV 186

Query: 184 VDALYFCIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYL 243
           VD LYFCIVTMCTIGYGDITPNS  TKLFS++FVLVGFGFIDILLSGMVSYVLDLQESY+
Sbjct: 187 VDGLYFCIVTMCTIGYGDITPNSVVTKLFSIMFVLVGFGFIDILLSGMVSYVLDLQESYM 246

Query: 244 LRNVKRGVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDS 303
           L + KR  + E  +SYIIDVKKGRMRIR+KVALALGVV+LCI VG+G+MHF+E +GWLDS
Sbjct: 247 LDSAKRRDEPEKRRSYIIDVKKGRMRIRLKVALALGVVVLCIAVGVGIMHFIEEIGWLDS 306

Query: 304 FYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKW 363
           FYLSVMSVTTVGYGD+AFK++ GR+FA+IWLLVSTLAVARAFLYLAEARVDKR+R  AK 
Sbjct: 307 FYLSVMSVTTVGYGDRAFKTLPGRLFAAIWLLVSTLAVARAFLYLAEARVDKRNRERAKK 366

Query: 364 ILGQDMTISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGK 423
           +L + M++S+F AADIDNNG VS++EYVIYKLKEM ++++KD++ IS  FD+LD    GK
Sbjct: 367 VLCETMSVSQFFAADIDNNGCVSKAEYVIYKLKEMEKITDKDILPISKQFDKLDRCSNGK 426

BLAST of MELO3C007158 vs. ExPASy Swiss-Prot
Match: Q9FL25 (Two-pore potassium channel 2 OS=Arabidopsis thaliana OX=3702 GN=TPK2 PE=2 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 3.4e-127
Identity = 255/430 (59.30%), Postives = 324/430 (75.35%), Query Frame = 0

Query: 4   EPLLPYL-SPRGKPPPPPPQLCPLPENDEITLPM--TPTEFKDRLIFGP-SSSPQDASPI 63
           +PL  YL +PR    PPPP L  LPEN+++T+PM  TP E K+RLIFG    S +++S  
Sbjct: 12  DPLRQYLMNPRIN--PPPPSLLTLPENNDVTIPMPITPLELKNRLIFGSFVRSRKESSLP 71

Query: 64  FDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAM 123
            DAL+ + S+S  SA++S  D +  L P ++             + P  N HRSKTAPAM
Sbjct: 72  IDALSQNPSTS-SSATTSFSDSTDLLLPLTEPNK------PVRKSKPTINFHRSKTAPAM 131

Query: 124 AVINDVNHSQEPK--PQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDA 183
           A IN+++H  +PK   Q   ++I+ QAV LL+VYLSLGVLIYWLNRD++  ++THPVVDA
Sbjct: 132 AAINNISHPNDPKTDQQSDSKTIVNQAVALLVVYLSLGVLIYWLNRDSYNVKQTHPVVDA 191

Query: 184 LYFCIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRN 243
           LYFCIVTMCTIGYGDITP+S  TKLFS+ FVLVGFGF+DILLSGMV+YVLDLQE+Y+L  
Sbjct: 192 LYFCIVTMCTIGYGDITPDSVVTKLFSIFFVLVGFGFMDILLSGMVTYVLDLQENYMLET 251

Query: 244 VKRGV----KRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLD 303
            +        R+  +SYIIDVKKGRMRIR+KV LALGVV+LC+G G+ +MHFVE +GWLD
Sbjct: 252 ARNESLNLNDRDKVRSYIIDVKKGRMRIRLKVGLALGVVVLCLGFGVLIMHFVEKIGWLD 311

Query: 304 SFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAK 363
           SFY SVMSVTTVGYGD+AF ++ GR+ A++WLLVSTLAVARA L+LAE+RVDKR+R  AK
Sbjct: 312 SFYFSVMSVTTVGYGDRAFNTLAGRLLAAMWLLVSTLAVARAILFLAESRVDKRNRERAK 371

Query: 364 WILGQDMTISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCG 423
            +LG+ M+IS+FL ADID NG VS++E+VIYKLK+M +++EKD+  I   FD+LD    G
Sbjct: 372 KVLGESMSISQFLDADIDCNGCVSKAEFVIYKLKKMDKITEKDINPIGFQFDKLDRTNSG 431

BLAST of MELO3C007158 vs. ExPASy Swiss-Prot
Match: Q69TN4 (Two pore potassium channel c OS=Oryza sativa subsp. japonica OX=39947 GN=TPKC PE=3 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 3.5e-116
Identity = 242/451 (53.66%), Postives = 306/451 (67.85%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPPPPPPQLCPLPENDEITL---PMTP-----TEFKDRLIFG---- 60
           M+ EPLL  LS      P P  L PLPE+ E++    P++P     + +K+R+IFG    
Sbjct: 1   MDTEPLLSPLS------PSPHLLHPLPEHAEVSTFSPPLSPCPSPASSYKERIIFGAHPP 60

Query: 61  ---PSSSPQDASPIFDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTW 120
              P   P    P        +S       S    PS P D  +   +  +     ++  
Sbjct: 61  PPPPPPPPPPPPPRGRRYYRRVSGDDLDVPSCSSSPSPPSDEENPPPNPPSLF---DFIG 120

Query: 121 PKSNLHRSKTAPAMAVINDV--------NHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVL 180
            ++NLHRS+TAPAMA +N            S+ P P   + +I+  A + L+ YL++GV 
Sbjct: 121 GRTNLHRSRTAPAMAPLNAAAIAAAAASGDSRNPPPPPRRPAIVLHAFLFLLAYLAMGVT 180

Query: 181 IYWLNRDNF--AAQETHPVVDALYFCIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFI 240
            Y     NF  +A  THPV DALYFCIVT+CTIGYGDITP + A KLFS+ FVL+GFGF+
Sbjct: 181 FYAALPGNFTSSAGPTHPVADALYFCIVTLCTIGYGDITPATPAAKLFSISFVLIGFGFV 240

Query: 241 DILLSGMVSYVLDLQESYLLRNVK--RGVKRESGKSYIIDVKKGRMRIRMKVALALGVVI 300
           DILLSGMVSYVLDLQE  L+  +K  R V R+   +YI D+KKGRMR+RMKVALAL VV 
Sbjct: 241 DILLSGMVSYVLDLQEHLLITALKNPRSV-RKHRHNYIFDLKKGRMRVRMKVALALTVVA 300

Query: 301 LCIGVGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVA 360
           +C+GVG  V+  VENLGWLD+ YL+VMSVTTVGYGD AF+++ GR+FAS WLLVSTLAVA
Sbjct: 301 ICVGVGAAVLKRVENLGWLDAVYLAVMSVTTVGYGDHAFRTLAGRLFASAWLLVSTLAVA 360

Query: 361 RAFLYLAEARVDKRHRMMAKWILGQDMTISEFLAADIDNNGFVSRSEYVIYKLKEMGRVS 420
           RAFLYLAE R+DKRHR MA W+L +DMT+SEFLAADIDNNG+V++SE+V+YKLKEMG++S
Sbjct: 361 RAFLYLAEMRIDKRHRAMANWVLSRDMTVSEFLAADIDNNGYVTKSEFVVYKLKEMGKIS 420

Query: 421 EKDVMQISNNFDRLDSGKCGKITLADLLESH 425
           EKD+M I + F R+DSG CGKITL+DLLESH
Sbjct: 421 EKDIMMICDQFQRMDSGNCGKITLSDLLESH 441

BLAST of MELO3C007158 vs. ExPASy Swiss-Prot
Match: Q9S6Z8 (Two-pore potassium channel 5 OS=Arabidopsis thaliana OX=3702 GN=TPK5 PE=1 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 3.4e-103
Identity = 222/422 (52.61%), Postives = 283/422 (67.06%), Query Frame = 0

Query: 17  PPPPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIFDALTLSLSSSRPSASS 76
           P P  +L P+PEN                   PSSS   AS     +T+  S S  S   
Sbjct: 7   PQPRFRLQPIPEN-------------------PSSSSSSAS-----ITIPRSISNTSFFH 66

Query: 77  SLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSN--------LHRSKTAPAMAVINDVNHS 136
            +      L  +   QS Q    D +    ++N        LHRS+TAPAM +I D+   
Sbjct: 67  EISQERLLLHHQDLEQSVQDDKEDQDSDSDETNRFLSQTRPLHRSRTAPAMVIIKDLRTK 126

Query: 137 --QEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYFCIVTMC 196
             +  KP    +SIIRQA+ LLIVYL+LGV IY  NRD+++  ETHPVVDALYFCIVTMC
Sbjct: 127 PPETKKPSPVSKSIIRQAIFLLIVYLTLGVSIYSFNRDHYSGIETHPVVDALYFCIVTMC 186

Query: 197 TIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK------- 256
           TIGYGDI P +  TK+F+V+FVL GFGF+DILLSG+V+YVLDLQES +L  ++       
Sbjct: 187 TIGYGDIAPLTPWTKIFAVVFVLFGFGFLDILLSGVVNYVLDLQESMILTGIQTRQHHQH 246

Query: 257 RGVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSV 316
               R S K YIID +KGRMRIRMKV LAL VV+LCIGVG  V+HFVE LG++DS YLSV
Sbjct: 247 HHHHRFSAKDYIIDFEKGRMRIRMKVCLALCVVVLCIGVGALVLHFVEELGFVDSVYLSV 306

Query: 317 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 376
           MSVTTVGYGD+AFK++ GR+FA++WLLVSTLAVARAFLYLAEAR+D+RHR   K  L ++
Sbjct: 307 MSVTTVGYGDRAFKTLQGRLFAAVWLLVSTLAVARAFLYLAEARIDRRHRKAVKLALNRE 366

Query: 377 MTISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGKITLAD 422
           +T+ + L AD   +GF+S+SEY++ KLKEMG++++KD+ Q+   F++LD  + GKITL D
Sbjct: 367 ITVDDLLKADTYQHGFISKSEYIVLKLKEMGKITQKDIDQVVIQFEKLDPNQIGKITLPD 404

BLAST of MELO3C007158 vs. ExPASy Swiss-Prot
Match: Q8LBL1 (Two-pore potassium channel 1 OS=Arabidopsis thaliana OX=3702 GN=TPK1 PE=1 SV=2)

HSP 1 Score: 213.4 bits (542), Expect = 5.0e-54
Identity = 124/350 (35.43%), Postives = 207/350 (59.14%), Query Frame = 0

Query: 83  TPLDP--RSQSQSQQAWLVDSNYTWPKSNLHRSKTAP-AMAVINDVNHSQEPKPQFGKQS 142
           TPL P  +  + +Q   L     +  K  L RS++AP    + ND     EP P   K  
Sbjct: 8   TPLLPTEKIDTMAQDFNLNSRTSSSRKRRLRRSRSAPRGDCMYNDDVKIDEPPPHPSKIP 67

Query: 143 I-------IRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYFCIVTMCTIGYGD 202
           +       +R+ ++ L +YL++G L ++L RD  +  +T  VVDALYFCIVTM T+GYGD
Sbjct: 68  MFSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCIVTMTTVGYGD 127

Query: 203 ITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKRGVKRESGKSYII 262
           + PNS+A++L +  FV  G   +  LLS    Y+++ QE+ L+R     +++  G + I+
Sbjct: 128 LVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFH--LRQSFGPTDIL 187

Query: 263 -DVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQA 322
            ++   ++R +      + VV+  +G    VM  VE +  + +FY    +VTT+GYGD++
Sbjct: 188 KELHTNKLRYKCYATCLVLVVLFIVGTIFLVM--VEKMPVISAFYCVCSTVTTLGYGDKS 247

Query: 323 FKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDMTISEFLAADID 382
           F S  GR+FA  W+L S++ +A+ FLY+AE   + + R + KW+L + +T ++  AAD+D
Sbjct: 248 FNSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDLEAADLD 307

Query: 383 NNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGKITLADLL 422
            +G V  +E+++YKLKEMG++ EKD+  I + F++LD  + G +T +D++
Sbjct: 308 EDGVVGAAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIV 353

BLAST of MELO3C007158 vs. ExPASy TrEMBL
Match: A0A1S3AYW0 (two-pore potassium channel 3 OS=Cucumis melo OX=3656 GN=LOC103484391 PE=3 SV=1)

HSP 1 Score: 826.6 bits (2134), Expect = 4.6e-236
Identity = 425/425 (100.00%), Postives = 425/425 (100.00%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPPPPPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60
           MEKEPLLPYLSPRGKPPPPPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF
Sbjct: 1   MEKEPLLPYLSPRGKPPPPPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120
           DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA
Sbjct: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYF 180
           VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYF
Sbjct: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300
           GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM
Sbjct: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGKITLADL 420
           TISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGKITLADL
Sbjct: 361 TISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGKITLADL 420

Query: 421 LESHH 426
           LESHH
Sbjct: 421 LESHH 425

BLAST of MELO3C007158 vs. ExPASy TrEMBL
Match: A0A6J1ED61 (two-pore potassium channel 3-like OS=Cucurbita moschata OX=3662 GN=LOC111433251 PE=3 SV=1)

HSP 1 Score: 781.9 bits (2018), Expect = 1.3e-222
Identity = 402/426 (94.37%), Postives = 414/426 (97.18%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPPPPPPQ-LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPI 60
           MEKEPLLPY+SPRGKP PPPPQ LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQD SPI
Sbjct: 1   MEKEPLLPYISPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAM 120
           FDALTLSLSSSRPSASSSLQDPSTPL P+SQS SQQAWLVDSNY+W KSNLHRSKTAPAM
Sbjct: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSNYSWHKSNLHRSKTAPAM 120

Query: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALY 180
           AVINDVNHSQEPKPQFGKQSIIRQAVVLLI+YLSLGVLIYW+NRD F+A ETHPVVDALY
Sbjct: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240
           FCIVTMCTIGYGDITPNS ATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK
Sbjct: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSV 300
           RGV+RESGKSYIIDVKKGRMRIRMKVALALGVVILCIG+GMGVMHFVENLGWLDSFYLSV
Sbjct: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGLGMGVMHFVENLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360
           MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGKITLAD 420
           MT+SEFLAAD DNNG VSRSEYV+YKLKEMG+VSEKDVMQISNNFDRLD+G CGKITLAD
Sbjct: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420

Query: 421 LLESHH 426
           LLESHH
Sbjct: 421 LLESHH 426

BLAST of MELO3C007158 vs. ExPASy TrEMBL
Match: A0A6J1IME3 (two-pore potassium channel 3-like OS=Cucurbita maxima OX=3661 GN=LOC111478359 PE=3 SV=1)

HSP 1 Score: 779.2 bits (2011), Expect = 8.5e-222
Identity = 401/426 (94.13%), Postives = 413/426 (96.95%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPPPPPPQ-LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPI 60
           MEKEPLLPY+SPRGKP PPPPQ LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQD SPI
Sbjct: 1   MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAM 120
           FDALTLSLSSSRPSASSSLQDPSTPL P+SQS SQQAWLVDSNY+W KSNLHRSKTAPAM
Sbjct: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSNYSWHKSNLHRSKTAPAM 120

Query: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALY 180
           AVINDVNHSQEPKPQFGK SIIRQAVVLLI+YLSLGVLIYW+NRD F+A ETHPVVDALY
Sbjct: 121 AVINDVNHSQEPKPQFGKLSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240
           FCIVTMCTIGYGDITPNS ATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK
Sbjct: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSV 300
           RGV+RESGKSYIIDVKKGRMRIRMKVALALGVVILCIG+GMGVMHFVENLGWLDSFYLSV
Sbjct: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGLGMGVMHFVENLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360
           MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGKITLAD 420
           MT+SEFLAAD DNNG VSRSEYV+YKLKEMG+VSEKDVMQISNNFDRLD+G CGKITLAD
Sbjct: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420

Query: 421 LLESHH 426
           LLESHH
Sbjct: 421 LLESHH 426

BLAST of MELO3C007158 vs. ExPASy TrEMBL
Match: A0A6J1CLR2 (two-pore potassium channel 3-like OS=Momordica charantia OX=3673 GN=LOC111012441 PE=3 SV=1)

HSP 1 Score: 778.9 bits (2010), Expect = 1.1e-221
Identity = 400/425 (94.12%), Postives = 411/425 (96.71%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPPPPPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60
           MEKEPLLPY SPR K PPP P LCPLPENDEITLP+TPTEFKDRLIFGPSSSPQDASPIF
Sbjct: 1   MEKEPLLPYQSPRAKLPPPIPLLCPLPENDEITLPLTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120
           DALTLSLSSSR S SSSL DPSTPLDP+SQSQSQQAWLVDSNY WPK+NLHRSKTAPAMA
Sbjct: 61  DALTLSLSSSRSSPSSSLYDPSTPLDPKSQSQSQQAWLVDSNYAWPKTNLHRSKTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYF 180
           VINDVNHSQE KPQFG QSIIRQAVVLLI+YLSLGVLIYW+N DNF A ETHPVVDALYF
Sbjct: 121 VINDVNHSQERKPQFGNQSIIRQAVVLLIIYLSLGVLIYWINLDNFTAHETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300
           GV+RESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVE+LGWLDSFYLSVM
Sbjct: 241 GVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVEDLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKS+TGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSLTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGKITLADL 420
           T+SEFLAADIDNNGFVSRSEYVIYKLKEMG+VSEKDVMQISNNFDRLD+GKCGKITLADL
Sbjct: 361 TVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDTGKCGKITLADL 420

Query: 421 LESHH 426
           LESHH
Sbjct: 421 LESHH 425

BLAST of MELO3C007158 vs. ExPASy TrEMBL
Match: A0A6J1EM72 (two-pore potassium channel 3-like OS=Cucurbita moschata OX=3662 GN=LOC111433827 PE=3 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.5e-213
Identity = 387/425 (91.06%), Postives = 406/425 (95.53%), Query Frame = 0

Query: 1   MEKEPLLPYLSPRGKPPPPPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60
           ME EPLLPY+SPRGK PP  PQL PLPENDEI LPMTPTEFKDRLIFGPSSSPQDAS  F
Sbjct: 1   METEPLLPYMSPRGK-PPSLPQLYPLPENDEIILPMTPTEFKDRLIFGPSSSPQDASSAF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120
           DALTLSL+S RPSASSS QDPSTPLDP+SQS SQQ WLVDS ++  KSNLHRSKTAPAMA
Sbjct: 61  DALTLSLASPRPSASSSRQDPSTPLDPKSQSPSQQGWLVDSKHSCHKSNLHRSKTAPAMA 120

Query: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYF 180
           VIND+NHSQEPKPQFGKQSIIRQAVVLLI+YLSLGV+IYW+NRDNF+A ETHPVVDALYF
Sbjct: 121 VINDINHSQEPKPQFGKQSIIRQAVVLLIIYLSLGVVIYWINRDNFSASETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240
           CIVTMCTIGYGDITPNS ATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR
Sbjct: 181 CIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300
           GV+RESGKSYIIDVKKGRMRIRMKVALALGVVILCIG+GMGVMHFVE+LGWLDSFYLSVM
Sbjct: 241 GVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGIGMGVMHFVEDLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360
           SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGKITLADL 420
           T+SEFLAAD DNNG VSRSEYV+YKLKEMG+VSEKDVMQISNNFDRLD+GKCGKITLADL
Sbjct: 361 TVSEFLAADTDNNGCVSRSEYVVYKLKEMGKVSEKDVMQISNNFDRLDTGKCGKITLADL 420

Query: 421 LESHH 426
           L++HH
Sbjct: 421 LQNHH 424

BLAST of MELO3C007158 vs. TAIR 10
Match: AT4G18160.1 (Ca2+ activated outward rectifying K+ channel 6 )

HSP 1 Score: 503.1 bits (1294), Expect = 2.3e-142
Identity = 279/428 (65.19%), Postives = 340/428 (79.44%), Query Frame = 0

Query: 4   EPLLPY-LSPRGKPPPPPPQLCPLPENDE--ITLPMTPTEFKDRLIFGP-SSSPQDASPI 63
           +PLL Y +SPR K   PP  L PLPE++E  I +PMTP+EFK+RLIFGP S SP+D+S  
Sbjct: 7   DPLLQYMISPRLK--KPPQLLFPLPEDNEVAIPMPMTPSEFKERLIFGPFSCSPRDSSHF 66

Query: 64  FDALTL-SLSSSRPSASSSLQDPST--PLDPRSQSQSQQAWLVDSNYTWPKSN-LHRSKT 123
            D++   S SSS  + ++   D ST  PL P    Q  + WL D   +  + + LHRSKT
Sbjct: 67  IDSMKQPSPSSSSTAVNNPFSDSSTLDPLLPPPPPQ-PEPWLSDQTSSHCQGHALHRSKT 126

Query: 124 APAMAVINDVNHS-QEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPV 183
           APAMAVIND++H  ++  P    +S++RQA  LL+VYLSLGVLIYWLNRD++   +THPV
Sbjct: 127 APAMAVINDLHHPIRQKDPTETSRSVVRQAFALLVVYLSLGVLIYWLNRDHYVVNQTHPV 186

Query: 184 VDALYFCIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYL 243
           VD LYFCIVTMCTIGYGDITPNS  TKLFS++FVLVGFGFIDILLSGMVSYVLDLQESY+
Sbjct: 187 VDGLYFCIVTMCTIGYGDITPNSVVTKLFSIMFVLVGFGFIDILLSGMVSYVLDLQESYM 246

Query: 244 LRNVKRGVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDS 303
           L + KR  + E  +SYIIDVKKGRMRIR+KVALALGVV+LCI VG+G+MHF+E +GWLDS
Sbjct: 247 LDSAKRRDEPEKRRSYIIDVKKGRMRIRLKVALALGVVVLCIAVGVGIMHFIEEIGWLDS 306

Query: 304 FYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKW 363
           FYLSVMSVTTVGYGD+AFK++ GR+FA+IWLLVSTLAVARAFLYLAEARVDKR+R  AK 
Sbjct: 307 FYLSVMSVTTVGYGDRAFKTLPGRLFAAIWLLVSTLAVARAFLYLAEARVDKRNRERAKK 366

Query: 364 ILGQDMTISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGK 423
           +L + M++S+F AADIDNNG VS++EYVIYKLKEM ++++KD++ IS  FD+LD    GK
Sbjct: 367 VLCETMSVSQFFAADIDNNGCVSKAEYVIYKLKEMEKITDKDILPISKQFDKLDRCSNGK 426

BLAST of MELO3C007158 vs. TAIR 10
Match: AT5G46370.1 (Ca2+ activated outward rectifying K+ channel 2 )

HSP 1 Score: 456.4 bits (1173), Expect = 2.4e-128
Identity = 255/430 (59.30%), Postives = 324/430 (75.35%), Query Frame = 0

Query: 4   EPLLPYL-SPRGKPPPPPPQLCPLPENDEITLPM--TPTEFKDRLIFGP-SSSPQDASPI 63
           +PL  YL +PR    PPPP L  LPEN+++T+PM  TP E K+RLIFG    S +++S  
Sbjct: 12  DPLRQYLMNPRIN--PPPPSLLTLPENNDVTIPMPITPLELKNRLIFGSFVRSRKESSLP 71

Query: 64  FDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAM 123
            DAL+ + S+S  SA++S  D +  L P ++             + P  N HRSKTAPAM
Sbjct: 72  IDALSQNPSTS-SSATTSFSDSTDLLLPLTEPNK------PVRKSKPTINFHRSKTAPAM 131

Query: 124 AVINDVNHSQEPK--PQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDA 183
           A IN+++H  +PK   Q   ++I+ QAV LL+VYLSLGVLIYWLNRD++  ++THPVVDA
Sbjct: 132 AAINNISHPNDPKTDQQSDSKTIVNQAVALLVVYLSLGVLIYWLNRDSYNVKQTHPVVDA 191

Query: 184 LYFCIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRN 243
           LYFCIVTMCTIGYGDITP+S  TKLFS+ FVLVGFGF+DILLSGMV+YVLDLQE+Y+L  
Sbjct: 192 LYFCIVTMCTIGYGDITPDSVVTKLFSIFFVLVGFGFMDILLSGMVTYVLDLQENYMLET 251

Query: 244 VKRGV----KRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLD 303
            +        R+  +SYIIDVKKGRMRIR+KV LALGVV+LC+G G+ +MHFVE +GWLD
Sbjct: 252 ARNESLNLNDRDKVRSYIIDVKKGRMRIRLKVGLALGVVVLCLGFGVLIMHFVEKIGWLD 311

Query: 304 SFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAK 363
           SFY SVMSVTTVGYGD+AF ++ GR+ A++WLLVSTLAVARA L+LAE+RVDKR+R  AK
Sbjct: 312 SFYFSVMSVTTVGYGDRAFNTLAGRLLAAMWLLVSTLAVARAILFLAESRVDKRNRERAK 371

Query: 364 WILGQDMTISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCG 423
            +LG+ M+IS+FL ADID NG VS++E+VIYKLK+M +++EKD+  I   FD+LD    G
Sbjct: 372 KVLGESMSISQFLDADIDCNGCVSKAEFVIYKLKKMDKITEKDINPIGFQFDKLDRTNSG 431

BLAST of MELO3C007158 vs. TAIR 10
Match: AT4G01840.1 (Ca2+ activated outward rectifying K+ channel 5 )

HSP 1 Score: 376.7 bits (966), Expect = 2.4e-104
Identity = 222/422 (52.61%), Postives = 283/422 (67.06%), Query Frame = 0

Query: 17  PPPPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIFDALTLSLSSSRPSASS 76
           P P  +L P+PEN                   PSSS   AS     +T+  S S  S   
Sbjct: 7   PQPRFRLQPIPEN-------------------PSSSSSSAS-----ITIPRSISNTSFFH 66

Query: 77  SLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSN--------LHRSKTAPAMAVINDVNHS 136
            +      L  +   QS Q    D +    ++N        LHRS+TAPAM +I D+   
Sbjct: 67  EISQERLLLHHQDLEQSVQDDKEDQDSDSDETNRFLSQTRPLHRSRTAPAMVIIKDLRTK 126

Query: 137 --QEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYFCIVTMC 196
             +  KP    +SIIRQA+ LLIVYL+LGV IY  NRD+++  ETHPVVDALYFCIVTMC
Sbjct: 127 PPETKKPSPVSKSIIRQAIFLLIVYLTLGVSIYSFNRDHYSGIETHPVVDALYFCIVTMC 186

Query: 197 TIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK------- 256
           TIGYGDI P +  TK+F+V+FVL GFGF+DILLSG+V+YVLDLQES +L  ++       
Sbjct: 187 TIGYGDIAPLTPWTKIFAVVFVLFGFGFLDILLSGVVNYVLDLQESMILTGIQTRQHHQH 246

Query: 257 RGVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSV 316
               R S K YIID +KGRMRIRMKV LAL VV+LCIGVG  V+HFVE LG++DS YLSV
Sbjct: 247 HHHHRFSAKDYIIDFEKGRMRIRMKVCLALCVVVLCIGVGALVLHFVEELGFVDSVYLSV 306

Query: 317 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 376
           MSVTTVGYGD+AFK++ GR+FA++WLLVSTLAVARAFLYLAEAR+D+RHR   K  L ++
Sbjct: 307 MSVTTVGYGDRAFKTLQGRLFAAVWLLVSTLAVARAFLYLAEARIDRRHRKAVKLALNRE 366

Query: 377 MTISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGKITLAD 422
           +T+ + L AD   +GF+S+SEY++ KLKEMG++++KD+ Q+   F++LD  + GKITL D
Sbjct: 367 ITVDDLLKADTYQHGFISKSEYIVLKLKEMGKITQKDIDQVVIQFEKLDPNQIGKITLPD 404

BLAST of MELO3C007158 vs. TAIR 10
Match: AT5G55630.1 (Outward rectifying potassium channel protein )

HSP 1 Score: 213.4 bits (542), Expect = 3.6e-55
Identity = 124/350 (35.43%), Postives = 207/350 (59.14%), Query Frame = 0

Query: 83  TPLDP--RSQSQSQQAWLVDSNYTWPKSNLHRSKTAP-AMAVINDVNHSQEPKPQFGKQS 142
           TPL P  +  + +Q   L     +  K  L RS++AP    + ND     EP P   K  
Sbjct: 8   TPLLPTEKIDTMAQDFNLNSRTSSSRKRRLRRSRSAPRGDCMYNDDVKIDEPPPHPSKIP 67

Query: 143 I-------IRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYFCIVTMCTIGYGD 202
           +       +R+ ++ L +YL++G L ++L RD  +  +T  VVDALYFCIVTM T+GYGD
Sbjct: 68  MFSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCIVTMTTVGYGD 127

Query: 203 ITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKRGVKRESGKSYII 262
           + PNS+A++L +  FV  G   +  LLS    Y+++ QE+ L+R     +++  G + I+
Sbjct: 128 LVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFH--LRQSFGPTDIL 187

Query: 263 -DVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQA 322
            ++   ++R +      + VV+  +G    VM  VE +  + +FY    +VTT+GYGD++
Sbjct: 188 KELHTNKLRYKCYATCLVLVVLFIVGTIFLVM--VEKMPVISAFYCVCSTVTTLGYGDKS 247

Query: 323 FKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDMTISEFLAADID 382
           F S  GR+FA  W+L S++ +A+ FLY+AE   + + R + KW+L + +T ++  AAD+D
Sbjct: 248 FNSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDLEAADLD 307

Query: 383 NNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGKITLADLL 422
            +G V  +E+++YKLKEMG++ EKD+  I + F++LD  + G +T +D++
Sbjct: 308 EDGVVGAAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIV 353

BLAST of MELO3C007158 vs. TAIR 10
Match: AT5G55630.2 (Outward rectifying potassium channel protein )

HSP 1 Score: 213.4 bits (542), Expect = 3.6e-55
Identity = 124/350 (35.43%), Postives = 207/350 (59.14%), Query Frame = 0

Query: 83  TPLDP--RSQSQSQQAWLVDSNYTWPKSNLHRSKTAP-AMAVINDVNHSQEPKPQFGKQS 142
           TPL P  +  + +Q   L     +  K  L RS++AP    + ND     EP P   K  
Sbjct: 8   TPLLPTEKIDTMAQDFNLNSRTSSSRKRRLRRSRSAPRGDCMYNDDVKIDEPPPHPSKIP 67

Query: 143 I-------IRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYFCIVTMCTIGYGD 202
           +       +R+ ++ L +YL++G L ++L RD  +  +T  VVDALYFCIVTM T+GYGD
Sbjct: 68  MFSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCIVTMTTVGYGD 127

Query: 203 ITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKRGVKRESGKSYII 262
           + PNS+A++L +  FV  G   +  LLS    Y+++ QE+ L+R     +++  G + I+
Sbjct: 128 LVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFH--LRQSFGPTDIL 187

Query: 263 -DVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQA 322
            ++   ++R +      + VV+  +G    VM  VE +  + +FY    +VTT+GYGD++
Sbjct: 188 KELHTNKLRYKCYATCLVLVVLFIVGTIFLVM--VEKMPVISAFYCVCSTVTTLGYGDKS 247

Query: 323 FKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDMTISEFLAADID 382
           F S  GR+FA  W+L S++ +A+ FLY+AE   + + R + KW+L + +T ++  AAD+D
Sbjct: 248 FNSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDLEAADLD 307

Query: 383 NNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGKITLADLL 422
            +G V  +E+++YKLKEMG++ EKD+  I + F++LD  + G +T +D++
Sbjct: 308 EDGVVGAAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIV 353

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008439665.19.6e-236100.00PREDICTED: two-pore potassium channel 3 [Cucumis melo][more]
XP_004134597.13.8e-23298.35two-pore potassium channel 3 [Cucumis sativus][more]
XP_038883350.13.3e-22896.47two-pore potassium channel 3-like [Benincasa hispida][more]
XP_023544618.12.7e-22294.37two-pore potassium channel 3-like [Cucurbita pepo subsp. pepo][more]
XP_022926002.12.7e-22294.37two-pore potassium channel 3-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9SVV63.2e-14165.19Two-pore potassium channel 3 OS=Arabidopsis thaliana OX=3702 GN=TPK3 PE=2 SV=1[more]
Q9FL253.4e-12759.30Two-pore potassium channel 2 OS=Arabidopsis thaliana OX=3702 GN=TPK2 PE=2 SV=1[more]
Q69TN43.5e-11653.66Two pore potassium channel c OS=Oryza sativa subsp. japonica OX=39947 GN=TPKC PE... [more]
Q9S6Z83.4e-10352.61Two-pore potassium channel 5 OS=Arabidopsis thaliana OX=3702 GN=TPK5 PE=1 SV=1[more]
Q8LBL15.0e-5435.43Two-pore potassium channel 1 OS=Arabidopsis thaliana OX=3702 GN=TPK1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A1S3AYW04.6e-236100.00two-pore potassium channel 3 OS=Cucumis melo OX=3656 GN=LOC103484391 PE=3 SV=1[more]
A0A6J1ED611.3e-22294.37two-pore potassium channel 3-like OS=Cucurbita moschata OX=3662 GN=LOC111433251 ... [more]
A0A6J1IME38.5e-22294.13two-pore potassium channel 3-like OS=Cucurbita maxima OX=3661 GN=LOC111478359 PE... [more]
A0A6J1CLR21.1e-22194.12two-pore potassium channel 3-like OS=Momordica charantia OX=3673 GN=LOC111012441... [more]
A0A6J1EM721.5e-21391.06two-pore potassium channel 3-like OS=Cucurbita moschata OX=3662 GN=LOC111433827 ... [more]
Match NameE-valueIdentityDescription
AT4G18160.12.3e-14265.19Ca2+ activated outward rectifying K+ channel 6 [more]
AT5G46370.12.4e-12859.30Ca2+ activated outward rectifying K+ channel 2 [more]
AT4G01840.12.4e-10452.61Ca2+ activated outward rectifying K+ channel 5 [more]
AT5G55630.13.6e-5535.43Outward rectifying potassium channel protein [more]
AT5G55630.23.6e-5535.43Outward rectifying potassium channel protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003280Two pore domain potassium channelPRINTSPR013332POREKCHANELcoord: 183..211
score: 35.69
coord: 303..312
score: 47.04
IPR003280Two pore domain potassium channelPANTHERPTHR11003POTASSIUM CHANNEL, SUBFAMILY Kcoord: 90..420
NoneNo IPR availableGENE3D1.10.287.70coord: 270..333
e-value: 1.4E-13
score: 52.6
NoneNo IPR availableGENE3D1.10.287.70coord: 137..237
e-value: 2.3E-21
score: 77.5
NoneNo IPR availableGENE3D1.10.238.10coord: 334..425
e-value: 1.2E-5
score: 27.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..58
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 10..26
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 71..93
NoneNo IPR availablePANTHERPTHR11003:SF282TWO-PORE POTASSIUM CHANNEL 3coord: 90..420
NoneNo IPR availableSUPERFAMILY81324Voltage-gated potassium channelscoord: 255..362
NoneNo IPR availableSUPERFAMILY81324Voltage-gated potassium channelscoord: 141..233
IPR013099Potassium channel domainPFAMPF07885Ion_trans_2coord: 272..340
e-value: 5.0E-11
score: 42.4
coord: 147..226
e-value: 4.3E-16
score: 58.6
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 369..381
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 359..422

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C007158.1MELO3C007158.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071805 potassium ion transmembrane transport
cellular_component GO:0016020 membrane
molecular_function GO:0005267 potassium channel activity