MELO3C006555 (gene) Melon (DHL92) v4

Overview
NameMELO3C006555
Typegene
OrganismCucumis melo cv. DHL92 (Melon (DHL92) v4)
DescriptionNEDD8-activating enzyme E1 catalytic subunit
Locationchr06: 4070736 .. 4074984 (-)
RNA-Seq ExpressionMELO3C006555
SyntenyMELO3C006555
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCGTAATAAACTACGATGTGATCTTATGATATGATCTTAGTTTTGTTAATTAAAATAATTTGTATACGTTTTTTACTTTATAAATAAATAAATAAAAACCGACGCGCGGGATTGGACTTTCACATCTCTTCCACAATCCAACCCGAACTAGCAAAGTGCCACTGCGGTTGAGGGCGCTGCGATTCAACAAACGGCGACTCAGTCCTGCGTGATAACCAAGCTACTTCCACTCTTCCACCCAACGCCCATTTCCTCTACCACCGGAAACCCCTTTTTACCTCTTTCTCCCCTTCTCTTTTATACTCCCAACAATTGCCTCCGGCAGTCCAGAGTCGGCGCTGTAAAGCATTCAAATGGCGGGCTCTGTCGTCCAATCCAGTCGATCGAGGGACCTCGATAAGCTCCTTCTTAGGCCCGGTAATCTCATCGGCGCCAATTTCGAGCCGGGTTCTCAAGTGGGTACTTCTTCTTCTCTGACAATTTTTCAAACACCTTGTGCTCACTACATGGTCTGCAATCGTCTTACGATTGTGTTGTTTTATATTGCAGTTGAGGGACGACCTTCAACAGTATGTGAAAGTGTTGGTCATAGGAGCTGGGGGGTTGGGCTGCGAGCTCCTCAAAGACTTGGCTTTGTCGGGTTTTCGGAATCTGGAAGTCATTGACATGGATCGCATCGAAGTTACTAATCTCAATCGTCAGTTTCTCTTCAGGTTTCAATTGTTTTCTTTGCTCTATTTGTATCTTTGGAGTTTTATTCGTAGTGGATTTTTTTAATTTTTTAACAACAGGGGCCCGGGGGTGGGTGTAGAACTCTCACATTTTGTAGGGTGTATACTGAGCTGTCGTGACATTTCAAATTGATCTCTGTTTTTAAGTTACATCCATTACTGACTGATGATATTTATATTTAGTAAAAATCTGCACTTGTTATAGGAATTCAATGGATCGAATGTCACATTTTGTCACATTTTACTTGACTGAAGCCTTTCTTCATAGTTTGCTTCCCGTTTTGTGGACTTCTCTTATTGTATGTCGACTCATACATTTTTCTTTTCTCAATGAAAGTCCAACTTTTACATCAAAAAAAAAAAAAAGAAGAAAAAAAAAAAAGATGGGTGTGTCGAATGTCCGTTCCTTTTAACCTTTTGTACTAGAATGCTGAACCAGCTGAATTAGCACGGTGCTTAAATAAATTTATCTGTAAGAAAGTTCTTTTCAGGATATTTTATTATTACGATATGATTTGACCTTGGAATCCTTGAGGAAATCACATTTCTCATATGTAAATCTGGAGATGGCTTTACAGTAGTCGTTTGATTAAGTACGGCAGTCAGAAGAATAACACTAAGGTAGCATTGCAGGCTTGAAGATGTTGGTAAGCCAAAAGCTGAGGTGGCTGCAAAACGTGTTATGGAGAGAGTTAGTGGTGTCAATATTCTGCCACATTTTTGCCGGATTGAGGACAAAGAAATTGAATTTTACAATGATTTTCACATAATCGCACTTGGTCTTGATTCAATTGAAGCTCGAAGTTACATCAATTCTGTTGCTTGTAGCTTCCTTGGTATGCTATTCTCTGACTTGCTATGTTACGTCTTTTTGCCATCTTGCTTTCATTAAATGTTAATGTCATGTTATTGATGATGACTGAGAACTTCATGTCCTTTATTTTCTCTTTGTAAATTTAGAGTATGATTCTGATGATAATCCATTAGAAGAAACAATTAAACCAATGGTAGATGGTGGAACTGAAGGCTTCAAGGGGCATGCAAGGGTAATTTTACCAGGGGTCACACCTTGCTTTGAGTGTACCATCTGGCTTTTTCCGCCTCAAGTAAAGTTTCCATTATGTACTCTAGCAGAAACTCCTAGAACTGCTGCTCATTGTATCGAGTATGCTCATCTAATTAAATGGGATGAGGTAATACATTATTACCAGGCTTCTCGTTGATAATTGTGAAAGTACCCCGTTTGGACTTACCAAGACTTTTTCTTTTAGGTTCATAGTGGAAAAGCTTTTGATCCTGATGATTCTGAACACATGAAATGGGTTTACAGTGAGGTATGATAATTCCTGTAGATCCGGTAATTCTTTTCTATTAGTGATTCTAAAATTCTAATTTATCTTCCCGCATCTATAGGCTCTTAAGAGGGCTGAGCTTTTTGGCATTCCGGGAGTTACATATTCACTAACTCAGGTACTTATTAGTCTTGGAATTTTCACATCACTGAAGTAATGGTTATTCTTTTTATCTGAACTTGTGGTTGTTCTGTTAGTGGGTATATATTATCTGCTTGCATCTTTTGTTAACTACTCACACGCTGTTGTTTACAAATTCCCCTACTGATATACATTTTCTAATGGTTTTTTAAGGGTGTTGTTAAGAATATCATACCTGCTATTGCTTCCACCAATGCAATTATATCAGCTGCATGTGCACTGGAAACGTTGAAGATTGTATCAGGATGCAGCAAAACTCTATCCAACTACTTAACGTGTGTACTGTTTTCTTTAACTTTGTGATAATTCCTTTTCATTTTAATGTTATACGGGTTCAATAATAATTTTATAGTGCAGCTTAATATTGTTTTTCTTTATTTCTTACCCATAAATATATGGGTACATCACATGTCAGGCTGCATTTAGAAGTTTTTTTATTATTATAGGTTCTTTTGGGAGCGCAATAACATAGTATTTAGAGGATTGGTGAGGAATCCTAGTGATGGTTCCTTCTTAGATTTCATGTTTTCCTCTAGGCTTCAATTTTGAAGATCATTTGTAATTATTCTCAGAGACATTTTTGTATGGCTGAACCCCCTTCCTTTAATGGCCCTTTCTTGTGTGGGTTTGGTTTTTCTCAAACCTTTTATTTATTTTTCAATGAAATTTTCTGTTTCCATTGAAAATACATTTCGATATATCGTGTAGCATTTTTTGAGTACTTCACTCATAATCCAGGAATGTGGTTTATGTTATGATTGCTACTTTTACATTTTCATTTGTTCTGGTTTTCATTATGGTCGAAAAGTTATATGGTCCTCTTCCATCATTCTTATTATTCTTGTTATTTGTATTCTTGAATTGATAAGTCTGTCAATGCCTTGTTAATATACTAAACACTTTACGGTTCCTTTCCATTATTTATTTTTTACACCTCTTAGATTAGAAGTCTAGCCTTCTATAGCCAATCTCTTCTTTCTATCGAGTGGGAAGTTTATATTACAAATATATTATTTCTATGTTGGAAGTTGAAGCTAGGCTTGCGTGTGAATGACACAATTTTCATAAATCATGTCTGTATTTGGAATAGCCTTTAACATTGTCCCATTATTTACTTTTATTTGTGACCATAATTTCCAGATGCATTGGATTTAATGCCAAGTGATCTTTTTTCAGATATAATGGTGCTGAAGGTCTCCACACTAAAGTGACCGAGTTTGTGAAAGATAAGGACTGTCTTGTTTGTGGTCCAGGCGTTCTTATTGAGCTGGATTCTTCCATTACTTTACAGAAGGTTGGTTATTTAGCTTATGCCATCCACATGTTATATGCAGCTCACAGACATATCCATCTTGTATTCAGTTGTTGCTGTGTTAGTAATTTCAGATTTTGAGAAATCTTGCTAATCCTTCATGACGGTTCCTCCCATTTTGAATTTGCTTGAAACAGTTCATTGACCTGCTAGAAGATTATCCCAAACTTTTGTTATCAAAGGCAAGTGTCACGCATCGAGGGAAGAATCTATACATGCAGGCACCTCCTGTACTTGAAGAGATGACCAGATCAAATTTGGGTGTTCCACTTTTTGACTTGATGGGTAAAGTTTTGAAGGACGTAGTCCATGTGACTGGTGTGGCTTCTAAGAATGATAAGAAAACTTCCTGTCTTCGAAAATTACGTGTGGCTTTTAAGGGGGTTGACGGTGTTACGGACATGGACACTGCAAGTGGGGCTTAATATTACATTGCATTTTTTATCTCCTAATTTGAGTCAGATGCCTCTCTCCACCCCTCTGACATGGAAATTAATGGAAAAAGGTTGATGTGGGGTCTGATCTATTCTCATTTTGTATAAACTTTCATTTCTTGTTCAAAGATTCCTGAAAAGAACAATAGAGCTGTGAATTTGTTGCCCGAGAAGACTGATGTATTGCACAATGATTTACTTCTTTCATCCCTCTACTTCAAGCGACCTTGTGAATATTAACTGCTAACAATGATTATGACTTAGCACAGCGGATATTTT

mRNA sequence

TCGTAATAAACTACGATGTGATCTTATGATATGATCTTAGTTTTGTTAATTAAAATAATTTGTATACGTTTTTTACTTTATAAATAAATAAATAAAAACCGACGCGCGGGATTGGACTTTCACATCTCTTCCACAATCCAACCCGAACTAGCAAAGTGCCACTGCGGTTGAGGGCGCTGCGATTCAACAAACGGCGACTCAGTCCTGCGTGATAACCAAGCTACTTCCACTCTTCCACCCAACGCCCATTTCCTCTACCACCGGAAACCCCTTTTTACCTCTTTCTCCCCTTCTCTTTTATACTCCCAACAATTGCCTCCGGCAGTCCAGAGTCGGCGCTGTAAAGCATTCAAATGGCGGGCTCTGTCGTCCAATCCAGTCGATCGAGGGACCTCGATAAGCTCCTTCTTAGGCCCGGTAATCTCATCGGCGCCAATTTCGAGCCGGGTTCTCAAGTGGGTACTTCTTCTTCTCTGACAATTTTTCAAACACCTTGTGCTCACTACATGGTCTGCAATCGTCTTACGATTGTGTTGTTTTATATTGCAGTTGAGGGACGACCTTCAACAGTATGTGAAAGTGTTGGTCATAGGAGCTGGGGGGTTGGGCTGCGAGCTCCTCAAAGACTTGGCTTTGTCGGGTTTTCGGAATCTGGAAGTCATTGACATGGATCGCATCGAAGTTACTAATCTCAATCGTCAGTTTCTCTTCAGGCTTGAAGATGTTGGTAAGCCAAAAGCTGAGGTGGCTGCAAAACGTGTTATGGAGAGAGTTAGTGGTGTCAATATTCTGCCACATTTTTGCCGGATTGAGGACAAAGAAATTGAATTTTACAATGATTTTCACATAATCGCACTTGGTCTTGATTCAATTGAAGCTCGAAGTTACATCAATTCTGTTGCTTGTAGCTTCCTTGAGTATGATTCTGATGATAATCCATTAGAAGAAACAATTAAACCAATGGTAGATGGTGGAACTGAAGGCTTCAAGGGGCATGCAAGGGTAATTTTACCAGGGGTCACACCTTGCTTTGAGTGTACCATCTGGCTTTTTCCGCCTCAAGTAAAGTTTCCATTATGTACTCTAGCAGAAACTCCTAGAACTGCTGCTCATTGTATCGAGTATGCTCATCTAATTAAATGGGATGAGGTTCATAGTGGAAAAGCTTTTGATCCTGATGATTCTGAACACATGAAATGGGTTTACAGTGAGGCTCTTAAGAGGGCTGAGCTTTTTGGCATTCCGGGAGTTACATATTCACTAACTCAGGGTGTTGTTAAGAATATCATACCTGCTATTGCTTCCACCAATGCAATTATATCAGCTGCATGTGCACTGGAAACGTTGAAGATTGTATCAGGATGCAGCAAAACTCTATCCAACTACTTAACATATAATGGTGCTGAAGGTCTCCACACTAAAGTGACCGAGTTTGTGAAAGATAAGGACTGTCTTGTTTGTGGTCCAGGCGTTCTTATTGAGCTGGATTCTTCCATTACTTTACAGAAGTTCATTGACCTGCTAGAAGATTATCCCAAACTTTTGTTATCAAAGGCAAGTGTCACGCATCGAGGGAAGAATCTATACATGCAGGCACCTCCTGTACTTGAAGAGATGACCAGATCAAATTTGGGTGTTCCACTTTTTGACTTGATGGGTAAAGTTTTGAAGGACGTAGTCCATGTGACTGGTGTGGCTTCTAAGAATGATAAGAAAACTTCCTGTCTTCGAAAATTACGTGTGGCTTTTAAGGGGGTTGACGGTGTTACGGACATGGACACTGCAAGTGGGGCTTAATATTACATTGCATTTTTTATCTCCTAATTTGAGTCAGATGCCTCTCTCCACCCCTCTGACATGGAAATTAATGGAAAAAGGTTGATGTGGGGTCTGATCTATTCTCATTTTGTATAAACTTTCATTTCTTGTTCAAAGATTCCTGAAAAGAACAATAGAGCTGTGAATTTGTTGCCCGAGAAGACTGATGTATTGCACAATGATTTACTTCTTTCATCCCTCTACTTCAAGCGACCTTGTGAATATTAACTGCTAACAATGATTATGACTTAGCACAGCGGATATTTT

Coding sequence (CDS)

ATGGATCGCATCGAAGTTACTAATCTCAATCGTCAGTTTCTCTTCAGGCTTGAAGATGTTGGTAAGCCAAAAGCTGAGGTGGCTGCAAAACGTGTTATGGAGAGAGTTAGTGGTGTCAATATTCTGCCACATTTTTGCCGGATTGAGGACAAAGAAATTGAATTTTACAATGATTTTCACATAATCGCACTTGGTCTTGATTCAATTGAAGCTCGAAGTTACATCAATTCTGTTGCTTGTAGCTTCCTTGAGTATGATTCTGATGATAATCCATTAGAAGAAACAATTAAACCAATGGTAGATGGTGGAACTGAAGGCTTCAAGGGGCATGCAAGGGTAATTTTACCAGGGGTCACACCTTGCTTTGAGTGTACCATCTGGCTTTTTCCGCCTCAAGTAAAGTTTCCATTATGTACTCTAGCAGAAACTCCTAGAACTGCTGCTCATTGTATCGAGTATGCTCATCTAATTAAATGGGATGAGGTTCATAGTGGAAAAGCTTTTGATCCTGATGATTCTGAACACATGAAATGGGTTTACAGTGAGGCTCTTAAGAGGGCTGAGCTTTTTGGCATTCCGGGAGTTACATATTCACTAACTCAGGGTGTTGTTAAGAATATCATACCTGCTATTGCTTCCACCAATGCAATTATATCAGCTGCATGTGCACTGGAAACGTTGAAGATTGTATCAGGATGCAGCAAAACTCTATCCAACTACTTAACATATAATGGTGCTGAAGGTCTCCACACTAAAGTGACCGAGTTTGTGAAAGATAAGGACTGTCTTGTTTGTGGTCCAGGCGTTCTTATTGAGCTGGATTCTTCCATTACTTTACAGAAGTTCATTGACCTGCTAGAAGATTATCCCAAACTTTTGTTATCAAAGGCAAGTGTCACGCATCGAGGGAAGAATCTATACATGCAGGCACCTCCTGTACTTGAAGAGATGACCAGATCAAATTTGGGTGTTCCACTTTTTGACTTGATGGGTAAAGTTTTGAAGGACGTAGTCCATGTGACTGGTGTGGCTTCTAAGAATGATAAGAAAACTTCCTGTCTTCGAAAATTACGTGTGGCTTTTAAGGGGGTTGACGGTGTTACGGACATGGACACTGCAAGTGGGGCTTAA

Protein sequence

MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDYPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGVTDMDTASGA
Homology
BLAST of MELO3C006555 vs. NCBI nr
Match: XP_008438709.1 (PREDICTED: NEDD8-activating enzyme E1 catalytic subunit [Cucumis melo])

HSP 1 Score: 763.8 bits (1971), Expect = 6.7e-217
Identity = 376/376 (100.00%), Postives = 376/376 (100.00%), Query Frame = 0

Query: 1   MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH 60
           MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH
Sbjct: 74  MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH 133

Query: 61  IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP 120
           IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP
Sbjct: 134 IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP 193

Query: 121 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVY 180
           CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVY
Sbjct: 194 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVY 253

Query: 181 SEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY 240
           SEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY
Sbjct: 254 SEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY 313

Query: 241 LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDYPKLLLSKASVT 300
           LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDYPKLLLSKASVT
Sbjct: 314 LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDYPKLLLSKASVT 373

Query: 301 HRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVA 360
           HRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVA
Sbjct: 374 HRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVA 433

Query: 361 FKGVDGVTDMDTASGA 377
           FKGVDGVTDMDTASGA
Sbjct: 434 FKGVDGVTDMDTASGA 449

BLAST of MELO3C006555 vs. NCBI nr
Match: KAA0049385.1 (NEDD8-activating enzyme E1 catalytic subunit [Cucumis melo var. makuwa] >TYK17174.1 NEDD8-activating enzyme E1 catalytic subunit [Cucumis melo var. makuwa])

HSP 1 Score: 762.3 bits (1967), Expect = 2.0e-216
Identity = 375/376 (99.73%), Postives = 375/376 (99.73%), Query Frame = 0

Query: 1   MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH 60
           MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH
Sbjct: 74  MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH 133

Query: 61  IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP 120
           IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP
Sbjct: 134 IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP 193

Query: 121 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVY 180
           CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVY
Sbjct: 194 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVY 253

Query: 181 SEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY 240
           SEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY
Sbjct: 254 SEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY 313

Query: 241 LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDYPKLLLSKASVT 300
           LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDYPKLLLSKASVT
Sbjct: 314 LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDYPKLLLSKASVT 373

Query: 301 HRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVA 360
           HRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRV 
Sbjct: 374 HRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVT 433

Query: 361 FKGVDGVTDMDTASGA 377
           FKGVDGVTDMDTASGA
Sbjct: 434 FKGVDGVTDMDTASGA 449

BLAST of MELO3C006555 vs. NCBI nr
Match: XP_004134149.1 (NEDD8-activating enzyme E1 catalytic subunit [Cucumis sativus] >KGN57012.1 hypothetical protein Csa_009619 [Cucumis sativus])

HSP 1 Score: 760.8 bits (1963), Expect = 5.7e-216
Identity = 374/376 (99.47%), Postives = 376/376 (100.00%), Query Frame = 0

Query: 1   MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH 60
           MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH
Sbjct: 74  MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH 133

Query: 61  IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP 120
           IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP
Sbjct: 134 IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP 193

Query: 121 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVY 180
           CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGK+FDPDDSEHMKWVY
Sbjct: 194 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKSFDPDDSEHMKWVY 253

Query: 181 SEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY 240
           SEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY
Sbjct: 254 SEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY 313

Query: 241 LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDYPKLLLSKASVT 300
           LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLED+PKLLLSKASVT
Sbjct: 314 LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDHPKLLLSKASVT 373

Query: 301 HRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVA 360
           HRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVA
Sbjct: 374 HRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVA 433

Query: 361 FKGVDGVTDMDTASGA 377
           FKGVDGVTDMDTASGA
Sbjct: 434 FKGVDGVTDMDTASGA 449

BLAST of MELO3C006555 vs. NCBI nr
Match: XP_038897781.1 (NEDD8-activating enzyme E1 catalytic subunit [Benincasa hispida])

HSP 1 Score: 758.1 bits (1956), Expect = 3.7e-215
Identity = 372/376 (98.94%), Postives = 375/376 (99.73%), Query Frame = 0

Query: 1   MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH 60
           MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNI+PHFCRIEDKEIEFYNDFH
Sbjct: 74  MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKEIEFYNDFH 133

Query: 61  IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP 120
           IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP
Sbjct: 134 IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP 193

Query: 121 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVY 180
           CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVY
Sbjct: 194 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVY 253

Query: 181 SEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY 240
           SEA+KRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY
Sbjct: 254 SEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY 313

Query: 241 LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDYPKLLLSKASVT 300
           LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLED+PKLLLSKASVT
Sbjct: 314 LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDHPKLLLSKASVT 373

Query: 301 HRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVA 360
           HRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKD VHVTGVASKNDKKTSCLRKLRVA
Sbjct: 374 HRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDAVHVTGVASKNDKKTSCLRKLRVA 433

Query: 361 FKGVDGVTDMDTASGA 377
           FKGVDGVTDMDTASGA
Sbjct: 434 FKGVDGVTDMDTASGA 449

BLAST of MELO3C006555 vs. NCBI nr
Match: XP_023528363.1 (NEDD8-activating enzyme E1 catalytic subunit [Cucurbita pepo subsp. pepo])

HSP 1 Score: 745.7 bits (1924), Expect = 1.9e-211
Identity = 366/376 (97.34%), Postives = 372/376 (98.94%), Query Frame = 0

Query: 1   MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH 60
           MDRIEVTNLNRQFLFRL+DVGKPKAEVAAKRVMERVSGVNI+ HFCRIEDKEIEFYNDFH
Sbjct: 74  MDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFH 133

Query: 61  IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP 120
           IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP
Sbjct: 134 IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP 193

Query: 121 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVY 180
           CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVY
Sbjct: 194 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVY 253

Query: 181 SEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY 240
           SEA+KRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAAC LETLKIVSGCSKTLSNY
Sbjct: 254 SEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNY 313

Query: 241 LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDYPKLLLSKASVT 300
           LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSI+LQKFI+LLED+PKLLLSKASVT
Sbjct: 314 LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLLSKASVT 373

Query: 301 HRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVA 360
           HRGKNLYMQAPPVLEEMTRSNL VPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVA
Sbjct: 374 HRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVA 433

Query: 361 FKGVDGVTDMDTASGA 377
           FKGVDGVTDMDTA GA
Sbjct: 434 FKGVDGVTDMDTAGGA 449

BLAST of MELO3C006555 vs. ExPASy Swiss-Prot
Match: O65041 (NEDD8-activating enzyme E1 catalytic subunit OS=Arabidopsis thaliana OX=3702 GN=ECR1 PE=1 SV=2)

HSP 1 Score: 624.4 bits (1609), Expect = 8.4e-178
Identity = 304/377 (80.64%), Postives = 331/377 (87.80%), Query Frame = 0

Query: 1   MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH 60
           MDRIEVTNLNRQFLFR+EDVGKPKAEVAAKRVMERVSGV I+PHF RIEDKEIEFYNDF+
Sbjct: 78  MDRIEVTNLNRQFLFRIEDVGKPKAEVAAKRVMERVSGVEIVPHFSRIEDKEIEFYNDFN 137

Query: 61  IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP 120
           IIALGLDSIEAR YIN VAC FLEY+ DD P  ETIKPMVDGGTEGFKGHARVILPGVTP
Sbjct: 138 IIALGLDSIEARKYINGVACGFLEYNEDDTPKRETIKPMVDGGTEGFKGHARVILPGVTP 197

Query: 121 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVY 180
           CFECTI+LFPPQVKFPLCTLAETPR AAHCIEYAHLI+W+ VH GK FDPD+ EHMKWVY
Sbjct: 198 CFECTIYLFPPQVKFPLCTLAETPRNAAHCIEYAHLIQWETVHRGKTFDPDEPEHMKWVY 257

Query: 181 SEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY 240
            EA++RAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVS CSKTL NY
Sbjct: 258 DEAIRRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSACSKTLVNY 317

Query: 241 LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDYPKLLLSKASVT 300
           LTYNG EGL+T+VT+F +D +CLVCGPG+LIELD+S+TL KFI++LED+PKLLLSKASV 
Sbjct: 318 LTYNGGEGLYTEVTKFERDTECLVCGPGILIELDTSVTLSKFIEMLEDHPKLLLSKASVK 377

Query: 301 HRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGV-ASKNDKKTSCLRKLRV 360
                LYMQAPPVLEE  R  L  PL+DLMG+V KD +HV G  A KN++K SC  K+RV
Sbjct: 378 QGENTLYMQAPPVLEEFHRPKLSKPLYDLMGRVQKDTIHVFGQRALKNNEKESCTTKVRV 437

Query: 361 AFKGVDGVTDMDTASGA 377
            FKG DGV DMDTA GA
Sbjct: 438 VFKGADGVADMDTAIGA 454

BLAST of MELO3C006555 vs. ExPASy Swiss-Prot
Match: Q9V6U8 (Nedd8-activating enzyme E1 catalytic subunit OS=Drosophila melanogaster OX=7227 GN=Uba3 PE=1 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 9.8e-86
Identity = 164/338 (48.52%), Postives = 218/338 (64.50%), Query Frame = 0

Query: 1   MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH 60
           MD IE++NLNRQFLFR  D+G  KAE AA+ +  RV    + PHF +I+D +  FY  FH
Sbjct: 80  MDTIELSNLNRQFLFRRTDIGASKAECAARFINARVPTCRVTPHFKKIQDFDESFYQQFH 139

Query: 61  IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP 120
           ++  GLDSI AR +IN +  S L Y+ D      +I PM+DGGTEGFKG+ARVILPG T 
Sbjct: 140 LVVCGLDSIVARRWINGMLLSMLRYEEDGTIDTSSIVPMIDGGTEGFKGNARVILPGFTA 199

Query: 121 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHS-GKAFDPDDSEHMKWV 180
           C ECT+ LFPPQV +PLCT+A TPR   HCIEY  +I+W++ +  G   D DD +H+ W+
Sbjct: 200 CIECTLDLFPPQVNYPLCTIANTPRLPEHCIEYVKIIQWEKQNPFGVPLDGDDPQHIGWI 259

Query: 181 YSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSN 240
           Y  AL+R+  F I GVTY L QGVVK+IIPA+ASTNA I+AACALE  K+ + C  +++N
Sbjct: 260 YERALERSNEFNITGVTYRLVQGVVKHIIPAVASTNAAIAAACALEVFKLATSCYDSMAN 319

Query: 241 YLTYNGAEGLHTKVTEFVKDKDCLVCG--PGVL-IELDSSITLQKFIDLLEDYPKLLLSK 300
           YL +N  +G++T   E  K ++CL C   P  L IE  ++ TL+  I LL D P+  L  
Sbjct: 320 YLNFNDLDGIYTYTYEAEKSENCLACSNTPQPLPIEDPNTTTLEDVIKLLCDSPRFQLKS 379

Query: 301 ASVT-----HRGKNLYMQAPPVLEEMTRSNLGVPLFDL 330
            ++T      + + LYM     +EE TR NL   L +L
Sbjct: 380 PALTTVMKDGKRRTLYMSGVKSIEEATRKNLTQSLGEL 417

BLAST of MELO3C006555 vs. ExPASy Swiss-Prot
Match: Q54QG9 (NEDD8-activating enzyme E1 catalytic subunit OS=Dictyostelium discoideum OX=44689 GN=uba3 PE=1 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 3.2e-84
Identity = 162/335 (48.36%), Postives = 215/335 (64.18%), Query Frame = 0

Query: 1   MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH 60
           MD I+++NLNRQFLFR +DVGK KAEVAA  +  R++G N+ PH CRI+DK+ ++Y  F 
Sbjct: 81  MDTIDISNLNRQFLFRRKDVGKSKAEVAAAFINSRITGCNVTPHKCRIQDKDEDYYRQFK 140

Query: 61  IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP 120
           I+  GLDSIEAR +IN +  + +  +   +   +TI P+VDGGTEGFKG ARVILP ++ 
Sbjct: 141 IVIAGLDSIEARRWINGLLVNLVVVNDSGDIEPDTIIPLVDGGTEGFKGQARVILPKISS 200

Query: 121 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDP-----DDSEH 180
           CFEC++  FPPQV + +CT+A TPR   HCI++A L    +    K FDP     D+ +H
Sbjct: 201 CFECSLDAFPPQVSYAICTIANTPRVPEHCIQWALLFGLQDATLEKPFDPKQFDNDNPDH 260

Query: 181 MKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSK 240
           M W++  A KRAE F I GVTY LTQGV KNIIPAIASTNAII+AAC  E  K  +  S 
Sbjct: 261 MNWLFECAKKRAEKFNINGVTYKLTQGVAKNIIPAIASTNAIIAAACCNEVFKFCTDSSG 320

Query: 241 TLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPG-VLIELDSSITLQKFIDLLEDYPKLLL 300
            L+NY+ YNG  G++T   E+   + C VCG   V  E+D S TL  F++ +    +   
Sbjct: 321 YLNNYMMYNGLNGVYTFTFEYEIKEGCAVCGTNLVTFEIDKSNTLSTFLEKITTDSRFQF 380

Query: 301 SKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDL 330
            K S+   G+NLYMQ   +L + T  NL   L +L
Sbjct: 381 KKPSLRSNGRNLYMQG--LLHQSTVPNLEKTLSEL 413

BLAST of MELO3C006555 vs. ExPASy Swiss-Prot
Match: Q8TBC4 (NEDD8-activating enzyme E1 catalytic subunit OS=Homo sapiens OX=9606 GN=UBA3 PE=1 SV=2)

HSP 1 Score: 312.4 bits (799), Expect = 7.0e-84
Identity = 162/338 (47.93%), Postives = 221/338 (65.38%), Query Frame = 0

Query: 1   MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH 60
           MD I+V+NLNRQFLFR +D+G+PKAEVAA+ + +RV   N++PHF +I+D    FY  FH
Sbjct: 101 MDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFH 160

Query: 61  IIALGLDSIEARSYINSVACSFLEYDSDDNPLE-ETIKPMVDGGTEGFKGHARVILPGVT 120
           II  GLDSI AR +IN +  S L Y  +D  L+  +I P++DGGTEGFKG+ARVILPG+T
Sbjct: 161 IIVCGLDSIIARRWINGMLISLLNY--EDGVLDPSSIVPLIDGGTEGFKGNARVILPGMT 220

Query: 121 PCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVH---SGKAFDPDDSEHM 180
            C ECT+ L+PPQV FP+CT+A  PR   HCIEY  +++W +      G   D DD EH+
Sbjct: 221 ACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDDPEHI 280

Query: 181 KWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKT 240
           +W++ ++L+RA  + I GVTY LTQGVVK IIPA+ASTNA+I+A CA E  KI +     
Sbjct: 281 QWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIP 340

Query: 241 LSNYLTYNGAEGLHTKVTEFVKDKDCLVCGP-GVLIELDSSITLQKFIDLLEDYPKLLLS 300
           L+NYL +N  +GL+T   E  + ++C  C      I+   S  LQ+ +D L +   L + 
Sbjct: 341 LNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMK 400

Query: 301 KASVTH--RGKN--LYMQAPPVLEEMTRSNLGVPLFDL 330
             ++T    GKN  LY+Q+   +EE TR NL   L +L
Sbjct: 401 SPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKEL 436

BLAST of MELO3C006555 vs. ExPASy Swiss-Prot
Match: Q5R4A0 (NEDD8-activating enzyme E1 catalytic subunit OS=Pongo abelii OX=9601 GN=UBA3 PE=2 SV=2)

HSP 1 Score: 312.4 bits (799), Expect = 7.0e-84
Identity = 161/338 (47.63%), Postives = 221/338 (65.38%), Query Frame = 0

Query: 1   MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH 60
           MD I+V+NLNRQFLFR +D+G+PKAEVAA+ + +RV   N++PHF +I+D    FY  FH
Sbjct: 101 MDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFH 160

Query: 61  IIALGLDSIEARSYINSVACSFLEYDSDDNPLE-ETIKPMVDGGTEGFKGHARVILPGVT 120
           II  GLDSI AR +IN +  S L Y  +D  L+  +I P++DGGTEGFKG+ARVILPG+T
Sbjct: 161 IIVCGLDSIIARRWINGMLISLLNY--EDGVLDPSSIVPLIDGGTEGFKGNARVILPGMT 220

Query: 121 PCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVH---SGKAFDPDDSEHM 180
            C ECT+ L+PPQV FP+CT+A  PR   HCIEY  +++W +      G   D DD EH+
Sbjct: 221 ACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDDPEHI 280

Query: 181 KWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKT 240
           +W++ ++L+RA  + I GVTY LTQGVVK IIPA+ASTNA+++A CA E  KI +     
Sbjct: 281 QWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVVAAVCATEVFKIATSAYIP 340

Query: 241 LSNYLTYNGAEGLHTKVTEFVKDKDCLVCGP-GVLIELDSSITLQKFIDLLEDYPKLLLS 300
           L+NYL +N  +GL+T   E  + ++C  C      I+   S  LQ+ +D L +   L + 
Sbjct: 341 LNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDFLTNSASLQMK 400

Query: 301 KASVTH--RGKN--LYMQAPPVLEEMTRSNLGVPLFDL 330
             ++T    GKN  LY+Q+   +EE TR NL   L +L
Sbjct: 401 SPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKEL 436

BLAST of MELO3C006555 vs. ExPASy TrEMBL
Match: A0A1S3AXQ8 (NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis melo OX=3656 GN=LOC103483734 PE=3 SV=1)

HSP 1 Score: 763.8 bits (1971), Expect = 3.3e-217
Identity = 376/376 (100.00%), Postives = 376/376 (100.00%), Query Frame = 0

Query: 1   MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH 60
           MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH
Sbjct: 74  MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH 133

Query: 61  IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP 120
           IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP
Sbjct: 134 IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP 193

Query: 121 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVY 180
           CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVY
Sbjct: 194 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVY 253

Query: 181 SEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY 240
           SEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY
Sbjct: 254 SEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY 313

Query: 241 LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDYPKLLLSKASVT 300
           LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDYPKLLLSKASVT
Sbjct: 314 LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDYPKLLLSKASVT 373

Query: 301 HRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVA 360
           HRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVA
Sbjct: 374 HRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVA 433

Query: 361 FKGVDGVTDMDTASGA 377
           FKGVDGVTDMDTASGA
Sbjct: 434 FKGVDGVTDMDTASGA 449

BLAST of MELO3C006555 vs. ExPASy TrEMBL
Match: A0A5D3D1R8 (NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00180 PE=3 SV=1)

HSP 1 Score: 762.3 bits (1967), Expect = 9.5e-217
Identity = 375/376 (99.73%), Postives = 375/376 (99.73%), Query Frame = 0

Query: 1   MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH 60
           MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH
Sbjct: 74  MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH 133

Query: 61  IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP 120
           IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP
Sbjct: 134 IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP 193

Query: 121 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVY 180
           CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVY
Sbjct: 194 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVY 253

Query: 181 SEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY 240
           SEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY
Sbjct: 254 SEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY 313

Query: 241 LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDYPKLLLSKASVT 300
           LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDYPKLLLSKASVT
Sbjct: 314 LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDYPKLLLSKASVT 373

Query: 301 HRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVA 360
           HRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRV 
Sbjct: 374 HRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVT 433

Query: 361 FKGVDGVTDMDTASGA 377
           FKGVDGVTDMDTASGA
Sbjct: 434 FKGVDGVTDMDTASGA 449

BLAST of MELO3C006555 vs. ExPASy TrEMBL
Match: A0A0A0L8U2 (NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis sativus OX=3659 GN=Csa_3G149920 PE=3 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 2.8e-216
Identity = 374/376 (99.47%), Postives = 376/376 (100.00%), Query Frame = 0

Query: 1   MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH 60
           MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH
Sbjct: 74  MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH 133

Query: 61  IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP 120
           IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP
Sbjct: 134 IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP 193

Query: 121 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVY 180
           CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGK+FDPDDSEHMKWVY
Sbjct: 194 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKSFDPDDSEHMKWVY 253

Query: 181 SEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY 240
           SEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY
Sbjct: 254 SEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY 313

Query: 241 LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDYPKLLLSKASVT 300
           LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLED+PKLLLSKASVT
Sbjct: 314 LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDHPKLLLSKASVT 373

Query: 301 HRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVA 360
           HRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVA
Sbjct: 374 HRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVA 433

Query: 361 FKGVDGVTDMDTASGA 377
           FKGVDGVTDMDTASGA
Sbjct: 434 FKGVDGVTDMDTASGA 449

BLAST of MELO3C006555 vs. ExPASy TrEMBL
Match: A0A6J1CA17 (NEDD8-activating enzyme E1 catalytic subunit OS=Momordica charantia OX=3673 GN=LOC111009338 PE=3 SV=1)

HSP 1 Score: 743.0 bits (1917), Expect = 6.0e-211
Identity = 363/376 (96.54%), Postives = 371/376 (98.67%), Query Frame = 0

Query: 1   MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH 60
           MDRIEVTNLNRQFLFRL+DVGKPKAEVAAKRVMERVSGVNI+PHFCRIEDKEIEFYNDFH
Sbjct: 74  MDRIEVTNLNRQFLFRLQDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKEIEFYNDFH 133

Query: 61  IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP 120
           IIALGLDSIEARSYIN+VACSFLEYDSDD+PLEETIKPMVDGGTEGFKGHARVILPGVTP
Sbjct: 134 IIALGLDSIEARSYINAVACSFLEYDSDDSPLEETIKPMVDGGTEGFKGHARVILPGVTP 193

Query: 121 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVY 180
           CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGK FDPDD  HMKWVY
Sbjct: 194 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKTFDPDDPVHMKWVY 253

Query: 181 SEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY 240
           +EA+KRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY
Sbjct: 254 NEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY 313

Query: 241 LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDYPKLLLSKASVT 300
           LTYNGAEGLHTKVTEFV+DKDCLVCGPGVLIELDSSITLQKFIDLLED+PKLLLSKASVT
Sbjct: 314 LTYNGAEGLHTKVTEFVRDKDCLVCGPGVLIELDSSITLQKFIDLLEDHPKLLLSKASVT 373

Query: 301 HRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVA 360
           HRGKNLYMQAPPVLEEMTRSNL VPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVA
Sbjct: 374 HRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVA 433

Query: 361 FKGVDGVTDMDTASGA 377
           FKGVDGVTDMDTA GA
Sbjct: 434 FKGVDGVTDMDTAGGA 449

BLAST of MELO3C006555 vs. ExPASy TrEMBL
Match: A0A6J1IQZ5 (NEDD8-activating enzyme E1 catalytic subunit OS=Cucurbita maxima OX=3661 GN=LOC111479191 PE=3 SV=1)

HSP 1 Score: 741.9 bits (1914), Expect = 1.3e-210
Identity = 364/376 (96.81%), Postives = 370/376 (98.40%), Query Frame = 0

Query: 1   MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH 60
           MDRIEVTNLNRQFLFRL+DVGKPKAEVAAKRVMERVSGVNI+ HFCRIEDKEIEFYNDFH
Sbjct: 74  MDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFH 133

Query: 61  IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP 120
           IIALGLDSIEARSYINSVACSFLEYDSDDNP EETIKPMVDGGTEGFKGHARVILPGVTP
Sbjct: 134 IIALGLDSIEARSYINSVACSFLEYDSDDNPFEETIKPMVDGGTEGFKGHARVILPGVTP 193

Query: 121 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVY 180
           CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVY
Sbjct: 194 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVY 253

Query: 181 SEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY 240
           SEA+KRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAAC LETLKIVSGCSKTLSNY
Sbjct: 254 SEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNY 313

Query: 241 LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDYPKLLLSKASVT 300
           LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSI+LQKFI+LLED+PKLLLSKASVT
Sbjct: 314 LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLLSKASVT 373

Query: 301 HRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVA 360
           HRGKNLYMQAPPVLEEMTRSNL VPLFDLMGKV KDVVHVTGVASKNDKKTSCLRKLRVA
Sbjct: 374 HRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVWKDVVHVTGVASKNDKKTSCLRKLRVA 433

Query: 361 FKGVDGVTDMDTASGA 377
           FKGVDGVTDMDTA GA
Sbjct: 434 FKGVDGVTDMDTAGGA 449

BLAST of MELO3C006555 vs. TAIR 10
Match: AT5G19180.1 (E1 C-terminal related 1 )

HSP 1 Score: 624.4 bits (1609), Expect = 6.0e-179
Identity = 304/377 (80.64%), Postives = 331/377 (87.80%), Query Frame = 0

Query: 1   MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFH 60
           MDRIEVTNLNRQFLFR+EDVGKPKAEVAAKRVMERVSGV I+PHF RIEDKEIEFYNDF+
Sbjct: 78  MDRIEVTNLNRQFLFRIEDVGKPKAEVAAKRVMERVSGVEIVPHFSRIEDKEIEFYNDFN 137

Query: 61  IIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP 120
           IIALGLDSIEAR YIN VAC FLEY+ DD P  ETIKPMVDGGTEGFKGHARVILPGVTP
Sbjct: 138 IIALGLDSIEARKYINGVACGFLEYNEDDTPKRETIKPMVDGGTEGFKGHARVILPGVTP 197

Query: 121 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVY 180
           CFECTI+LFPPQVKFPLCTLAETPR AAHCIEYAHLI+W+ VH GK FDPD+ EHMKWVY
Sbjct: 198 CFECTIYLFPPQVKFPLCTLAETPRNAAHCIEYAHLIQWETVHRGKTFDPDEPEHMKWVY 257

Query: 181 SEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNY 240
            EA++RAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVS CSKTL NY
Sbjct: 258 DEAIRRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSACSKTLVNY 317

Query: 241 LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDYPKLLLSKASVT 300
           LTYNG EGL+T+VT+F +D +CLVCGPG+LIELD+S+TL KFI++LED+PKLLLSKASV 
Sbjct: 318 LTYNGGEGLYTEVTKFERDTECLVCGPGILIELDTSVTLSKFIEMLEDHPKLLLSKASVK 377

Query: 301 HRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGV-ASKNDKKTSCLRKLRV 360
                LYMQAPPVLEE  R  L  PL+DLMG+V KD +HV G  A KN++K SC  K+RV
Sbjct: 378 QGENTLYMQAPPVLEEFHRPKLSKPLYDLMGRVQKDTIHVFGQRALKNNEKESCTTKVRV 437

Query: 361 AFKGVDGVTDMDTASGA 377
            FKG DGV DMDTA GA
Sbjct: 438 VFKGADGVADMDTAIGA 454

BLAST of MELO3C006555 vs. TAIR 10
Match: AT2G21470.1 (SUMO-activating enzyme 2 )

HSP 1 Score: 106.3 bits (264), Expect = 5.4e-23
Identity = 58/173 (33.53%), Postives = 91/173 (52.60%), Query Frame = 0

Query: 1   MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKE--IEFYND 60
           MD IEV+NLNRQFLFR   VG+ KA+VA   V+     +NI  +   +++ E  ++F+  
Sbjct: 44  MDTIEVSNLNRQFLFRRSHVGQSKAKVARDAVLRFRPNINIRSYHANVKNPEFDVDFFKQ 103

Query: 61  FHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGV 120
           F ++  GLD+++AR ++N +  +                P+V+ GT GF G   V + G 
Sbjct: 104 FDVVLNGLDNLDARRHVNRLCLA-------------ADVPLVESGTTGFLGQVTVHIKGK 163

Query: 121 TPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPD 172
           T C+EC     P    +P+CT+  TP    HCI +A  + + ++   K  D D
Sbjct: 164 TECYECQ--TKPAPKTYPVCTITSTPTKFVHCIVWAKDLLFAKLFGDKNQDND 201


HSP 2 Score: 52.4 bits (124), Expect = 9.3e-07
Identity = 44/162 (27.16%), Postives = 76/162 (46.91%), Query Frame = 0

Query: 168 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 227
           FD DD   +++V + A  RAE FGIP  +    +G+  NI+ A+A+TNAII+    +E +
Sbjct: 336 FDKDDQLAVEFVTAAANIRAESFGIPLHSLFEAKGIAGNIVHAVATTNAIIAGLIVIEAI 395

Query: 228 KIV-SGCSKTLSNY-LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDL 287
           K++     K    Y L +   + L   +  +  +  C VC    L+ L+ +    K  DL
Sbjct: 396 KVLKKDVDKFRMTYCLEHPSKKLLLMPIEPYEPNPACYVCSETPLV-LEINTRKSKLRDL 455

Query: 288 LEDYPKLLLS-KASVTHRGKNLYMQAPPVLEEMTRSNLGVPL 327
           ++   K  L     +   G +L  +    L+++  +N    L
Sbjct: 456 VDKIVKTKLGMNLPLIMHGNSLLYEVGDDLDDIMVANYNANL 496

BLAST of MELO3C006555 vs. TAIR 10
Match: AT2G21470.2 (SUMO-activating enzyme 2 )

HSP 1 Score: 106.3 bits (264), Expect = 5.4e-23
Identity = 58/173 (33.53%), Postives = 91/173 (52.60%), Query Frame = 0

Query: 1   MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKE--IEFYND 60
           MD IEV+NLNRQFLFR   VG+ KA+VA   V+     +NI  +   +++ E  ++F+  
Sbjct: 44  MDTIEVSNLNRQFLFRRSHVGQSKAKVARDAVLRFRPNINIRSYHANVKNPEFDVDFFKQ 103

Query: 61  FHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGV 120
           F ++  GLD+++AR ++N +  +                P+V+ GT GF G   V + G 
Sbjct: 104 FDVVLNGLDNLDARRHVNRLCLA-------------ADVPLVESGTTGFLGQVTVHIKGK 163

Query: 121 TPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPD 172
           T C+EC     P    +P+CT+  TP    HCI +A  + + ++   K  D D
Sbjct: 164 TECYECQ--TKPAPKTYPVCTITSTPTKFVHCIVWAKDLLFAKLFGDKNQDND 201


HSP 2 Score: 52.4 bits (124), Expect = 9.3e-07
Identity = 44/162 (27.16%), Postives = 76/162 (46.91%), Query Frame = 0

Query: 168 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 227
           FD DD   +++V + A  RAE FGIP  +    +G+  NI+ A+A+TNAII+    +E +
Sbjct: 336 FDKDDQLAVEFVTAAANIRAESFGIPLHSLFEAKGIAGNIVHAVATTNAIIAGLIVIEAI 395

Query: 228 KIV-SGCSKTLSNY-LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDL 287
           K++     K    Y L +   + L   +  +  +  C VC    L+ L+ +    K  DL
Sbjct: 396 KVLKKDVDKFRMTYCLEHPSKKLLLMPIEPYEPNPACYVCSETPLV-LEINTRKSKLRDL 455

Query: 288 LEDYPKLLLS-KASVTHRGKNLYMQAPPVLEEMTRSNLGVPL 327
           ++   K  L     +   G +L  +    L+++  +N    L
Sbjct: 456 VDKIVKTKLGMNLPLIMHGNSLLYEVGDDLDDIMVANYNANL 496

BLAST of MELO3C006555 vs. TAIR 10
Match: AT2G21470.3 (SUMO-activating enzyme 2 )

HSP 1 Score: 106.3 bits (264), Expect = 5.4e-23
Identity = 58/173 (33.53%), Postives = 91/173 (52.60%), Query Frame = 0

Query: 1   MDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKE--IEFYND 60
           MD IEV+NLNRQFLFR   VG+ KA+VA   V+     +NI  +   +++ E  ++F+  
Sbjct: 44  MDTIEVSNLNRQFLFRRSHVGQSKAKVARDAVLRFRPNINIRSYHANVKNPEFDVDFFKQ 103

Query: 61  FHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGV 120
           F ++  GLD+++AR ++N +  +                P+V+ GT GF G   V + G 
Sbjct: 104 FDVVLNGLDNLDARRHVNRLCLA-------------ADVPLVESGTTGFLGQVTVHIKGK 163

Query: 121 TPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPD 172
           T C+EC     P    +P+CT+  TP    HCI +A  + + ++   K  D D
Sbjct: 164 TECYECQ--TKPAPKTYPVCTITSTPTKFVHCIVWAKDLLFAKLFGDKNQDND 201


HSP 2 Score: 55.1 bits (131), Expect = 1.4e-07
Identity = 48/180 (26.67%), Postives = 81/180 (45.00%), Query Frame = 0

Query: 150 CIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIP 209
           C+E  HL           FD DD   +++V + A  RAE FGIP  +    +G+  NI+ 
Sbjct: 331 CVEIGHL----------TFDKDDQLAVEFVTAAANIRAESFGIPLHSLFEAKGIAGNIVH 390

Query: 210 AIASTNAIISAACALETLKIV-SGCSKTLSNY-LTYNGAEGLHTKVTEFVKDKDCLVCGP 269
           A+A+TNAII+    +E +K++     K    Y L +   + L   +  +  +  C VC  
Sbjct: 391 AVATTNAIIAGLIVIEAIKVLKKDVDKFRMTYCLEHPSKKLLLMPIEPYEPNPACYVCSE 450

Query: 270 GVLIELDSSITLQKFIDLLEDYPKLLLS-KASVTHRGKNLYMQAPPVLEEMTRSNLGVPL 327
             L+ L+ +    K  DL++   K  L     +   G +L  +    L+++  +N    L
Sbjct: 451 TPLV-LEINTRKSKLRDLVDKIVKTKLGMNLPLIMHGNSLLYEVGDDLDDIMVANYNANL 499

BLAST of MELO3C006555 vs. TAIR 10
Match: AT5G06460.1 (ubiquitin activating enzyme 2 )

HSP 1 Score: 70.5 bits (171), Expect = 3.3e-12
Identity = 46/158 (29.11%), Postives = 83/158 (52.53%), Query Frame = 0

Query: 2   DRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNI--LPHFCRIEDKEI---EFY 61
           D IE +NL+RQFLFR  ++G+ K+ VAA       S +NI  L +    E + +    F+
Sbjct: 527 DVIEKSNLSRQFLFRDWNIGQAKSTVAATAAAGINSRLNIDALQNRVGPETENVFDDSFW 586

Query: 62  NDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILP 121
            +  ++   LD++ AR Y++S  C + +            KP+++ GT G K + ++++P
Sbjct: 587 ENLTVVVNALDNVTARLYVDS-RCVYFQ------------KPLLESGTLGAKCNTQMVIP 646

Query: 122 GVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYA 155
            +T  +  +    PP+ + P+CT+   P    HC+ +A
Sbjct: 647 HLTENYGAS--RDPPEKQAPMCTVHSFPHNIDHCLTWA 669

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008438709.16.7e-217100.00PREDICTED: NEDD8-activating enzyme E1 catalytic subunit [Cucumis melo][more]
KAA0049385.12.0e-21699.73NEDD8-activating enzyme E1 catalytic subunit [Cucumis melo var. makuwa] >TYK1717... [more]
XP_004134149.15.7e-21699.47NEDD8-activating enzyme E1 catalytic subunit [Cucumis sativus] >KGN57012.1 hypot... [more]
XP_038897781.13.7e-21598.94NEDD8-activating enzyme E1 catalytic subunit [Benincasa hispida][more]
XP_023528363.11.9e-21197.34NEDD8-activating enzyme E1 catalytic subunit [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
O650418.4e-17880.64NEDD8-activating enzyme E1 catalytic subunit OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9V6U89.8e-8648.52Nedd8-activating enzyme E1 catalytic subunit OS=Drosophila melanogaster OX=7227 ... [more]
Q54QG93.2e-8448.36NEDD8-activating enzyme E1 catalytic subunit OS=Dictyostelium discoideum OX=4468... [more]
Q8TBC47.0e-8447.93NEDD8-activating enzyme E1 catalytic subunit OS=Homo sapiens OX=9606 GN=UBA3 PE=... [more]
Q5R4A07.0e-8447.63NEDD8-activating enzyme E1 catalytic subunit OS=Pongo abelii OX=9601 GN=UBA3 PE=... [more]
Match NameE-valueIdentityDescription
A0A1S3AXQ83.3e-217100.00NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A5D3D1R89.5e-21799.73NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis melo var. makuwa OX=1194... [more]
A0A0A0L8U22.8e-21699.47NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis sativus OX=3659 GN=Csa_3... [more]
A0A6J1CA176.0e-21196.54NEDD8-activating enzyme E1 catalytic subunit OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1IQZ51.3e-21096.81NEDD8-activating enzyme E1 catalytic subunit OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT5G19180.16.0e-17980.64E1 C-terminal related 1 [more]
AT2G21470.15.4e-2333.53SUMO-activating enzyme 2 [more]
AT2G21470.25.4e-2333.53SUMO-activating enzyme 2 [more]
AT2G21470.35.4e-2333.53SUMO-activating enzyme 2 [more]
AT5G06460.13.3e-1229.11ubiquitin activating enzyme 2 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014929E2 bindingSMARTSM01181E2_bind_2coord: 271..362
e-value: 5.1E-36
score: 135.7
IPR014929E2 bindingPFAMPF08825E2_bindcoord: 272..361
e-value: 2.6E-22
score: 78.7
NoneNo IPR availableGENE3D3.40.50.720coord: 1..269
e-value: 2.3E-47
score: 163.4
NoneNo IPR availableGENE3D3.10.290.20coord: 270..357
e-value: 1.4E-15
score: 58.9
NoneNo IPR availablePANTHERPTHR10953UBIQUITIN-ACTIVATING ENZYME E1coord: 1..341
NoneNo IPR availableCDDcd01488Uba3_RUBcoord: 1..266
e-value: 2.15292E-178
score: 495.337
IPR023318Ubiquitin activating enzyme, alpha domain superfamilyGENE3D1.10.10.520Ubiquitin activating enzymes (Uba3). Chain: B, domain 2coord: 138..206
e-value: 6.4E-32
score: 111.3
IPR000594THIF-type NAD/FAD binding foldPFAMPF00899ThiFcoord: 1..262
e-value: 1.6E-51
score: 175.1
IPR030468NEDD8-activating enzyme E1 catalytic subunitPANTHERPTHR10953:SF6NEDD8-ACTIVATING ENZYME E1 CATALYTIC SUBUNITcoord: 1..341
IPR033127Ubiquitin-activating enzyme E1, Cys active sitePROSITEPS00865UBIQUITIN_ACTIVAT_2coord: 136..144
IPR035985Ubiquitin-activating enzymeSUPERFAMILY69572Activating enzymes of the ubiquitin-like proteinscoord: 1..333

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C006555.1MELO3C006555.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045116 protein neddylation
molecular_function GO:0019781 NEDD8 activating enzyme activity
molecular_function GO:0008641 ubiquitin-like modifier activating enzyme activity