MELO3C006020.jh1 (gene) Melon (Harukei-3) v1.41

Overview
NameMELO3C006020.jh1
Typegene
OrganismCucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
Descriptionaspartyl protease family protein 2
Locationchr06: 1094747 .. 1096539 (+)
RNA-Seq ExpressionMELO3C006020.jh1
SyntenyMELO3C006020.jh1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonstart_codonCDSpolypeptidestop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCTTTGTCTTCCTAACAAAAAAAGATGAAAATGGAGGCAAACACCATTTCACTTCCCTTCATCTTCTTCCTCCTTCTCGCTATTCTCTCTCTCTCCACCGCCTTCTCCGATTTCCAAACCCTTATTCTCAGATCCCTTCCCTCCTCACCTTCATTCTTACCCTCCGATTCCAACTCCTTCCTTTCCTCCGAGGCTACCGAAACGGAGCTTGGTTTAGAACTGCACCTTCACCATTTGGATGCTCTGTCTTTCAATCGAACGCCGGAGGAGCTCTTCCACCTCCGCCTTCAAAGAGACGCTATACGAGTCAAGAAGTTGAGTTCACTCGGTGCTACCTCTCGAAATCTGAGCCGACCCAGTGGGACCACCGGCTTCAGTAGCTCCGTCATCTCCGGACTCGCTCAAGGCAGTGGCGAGTACTTCACGCGCATCGGCGTCGGCACGCCACCCAAGTATGTCTACATGGTGCTTGACACCGGCAGTGATATTGTTTGGCTACAGTGTGCTCCTTGTAAGAATTGCTACTCTCAGACTGACCCTGTTTTCAACCCGGTTAAGTCTGGATCCTTTGCCAAGGTTCTATGCCGAACGCCGCTGTGCCGTCGGCTTGAATCTCCGGGGTGCAACCAGCGTCAGACGTGTCTTTACCAAGTTTCTTACGGCGACGGTTCCTATACCACCGGCGAGTTCGTCACCGAAACCCTAACCTTCCGCCGGACTAAAGTGGAGCAAGTAGCCCTTGGTTGTGGCCACGATAATGAGGGTTTATTCGTTGGTGCGGCTGGGCTTTTAGGTCTTGGCCGGGGAGGGTTGTCGTTTCCGTCCCAAGCTGGCCGGACTTTCAACCAAAAATTTTCTTACTGCTTGGTGGACCGTTCCGCCTCTTCCAAACCGTCCTCCGTAGTCTTCGGCAACTCCGCCGTCTCTCGAACCGCCCGGTTCACTCCTCTCCTCACGAACCCTAGGTTGGACACGTTTTACTACGTTGAACTACTCGGAATCAGCGTCGGAGGTACGCCCGTCTCCGGCATCTCCTCTTCACATTTCAAGCTTGATCGGACCGGTAACGGTGGAGTAATAATCGATTGCGGTACTTCTGTTACTCGATTGAACAAACCAGCGTACATTGCTCTGCGCGACGCCTTCCGTGCTGGAGCTTCAAGTTTGAAATCGGCGCCAGAGTTTTCTCTCTTCGATACTTGCTACGATCTGTCTGGGAAGACGACGGTGAAGGTCCCGACAGTGGTGCTACATTTCAGAGGTGCTGATGTATCATTACCAGCGTCCAATTATCTAATCCCGGTCGACGGCAGCGGCCGATTTTGCTTCGCCTTCGCTGGAACTACCAGTGGGCTATCAATTATCGGCAACATCCAGCAGCAAGGATTTCGGGTGGTGTACGATTTGGCGAGTTCTCGGGTCGGATTTTCTCCTCGTGGTTGCGCCTAATTTCTTCGAACAGACAGCACGTAATGCGGGTTGCCGGAGAAATTCCTCCGCCGGCGTCTGTCCTAAAACCCTTCTTCCTCTTTTCCGACATGTAATAATAAAAAAGAAAGAAAAAAGAAAAAAAGGTGGAAGAAAAAGGGGTGCATAATTAGACTCCAACGGTGTCGTTTAACTTTTACTATTTTATTTAACTTTTTAGTTTTTTCATTCCAAATATCTCCATCTCTTTTCTTATTTAATTATATTTTCTATTCTATTGGTGTCGGTAAATTTATATATTAATTTCTCAAAAACATAAATATTTTTTTACTTTCTAAAATAAAGAGTCTTAATTGTTCAAGTT

mRNA sequence

ACCTTTGTCTTCCTAACAAAAAAAGATGAAAATGGAGGCAAACACCATTTCACTTCCCTTCATCTTCTTCCTCCTTCTCGCTATTCTCTCTCTCTCCACCGCCTTCTCCGATTTCCAAACCCTTATTCTCAGATCCCTTCCCTCCTCACCTTCATTCTTACCCTCCGATTCCAACTCCTTCCTTTCCTCCGAGGCTACCGAAACGGAGCTTGGTTTAGAACTGCACCTTCACCATTTGGATGCTCTGTCTTTCAATCGAACGCCGGAGGAGCTCTTCCACCTCCGCCTTCAAAGAGACGCTATACGAGTCAAGAAGTTGAGTTCACTCGGTGCTACCTCTCGAAATCTGAGCCGACCCAGTGGGACCACCGGCTTCAGTAGCTCCGTCATCTCCGGACTCGCTCAAGGCAGTGGCGAGTACTTCACGCGCATCGGCGTCGGCACGCCACCCAAGTATGTCTACATGGTGCTTGACACCGGCAGTGATATTGTTTGGCTACAGTGTGCTCCTTGTAAGAATTGCTACTCTCAGACTGACCCTGTTTTCAACCCGGTTAAGTCTGGATCCTTTGCCAAGGTTCTATGCCGAACGCCGCTGTGCCGTCGGCTTGAATCTCCGGGGTGCAACCAGCGTCAGACGTGTCTTTACCAAGTTTCTTACGGCGACGGTTCCTATACCACCGGCGAGTTCGTCACCGAAACCCTAACCTTCCGCCGGACTAAAGTGGAGCAAGTAGCCCTTGGTTGTGGCCACGATAATGAGGGTTTATTCGTTGGTGCGGCTGGGCTTTTAGGTCTTGGCCGGGGAGGGTTGTCGTTTCCGTCCCAAGCTGGCCGGACTTTCAACCAAAAATTTTCTTACTGCTTGGTGGACCGTTCCGCCTCTTCCAAACCGTCCTCCGTAGTCTTCGGCAACTCCGCCGTCTCTCGAACCGCCCGGTTCACTCCTCTCCTCACGAACCCTAGGTTGGACACGTTTTACTACGTTGAACTACTCGGAATCAGCGTCGGAGGTACGCCCGTCTCCGGCATCTCCTCTTCACATTTCAAGCTTGATCGGACCGGTAACGGTGGAGTAATAATCGATTGCGGTACTTCTGTTACTCGATTGAACAAACCAGCGTACATTGCTCTGCGCGACGCCTTCCGTGCTGGAGCTTCAAGTTTGAAATCGGCGCCAGAGTTTTCTCTCTTCGATACTTGCTACGATCTGTCTGGGAAGACGACGGTGAAGGTCCCGACAGTGGTGCTACATTTCAGAGGTGCTGATGTATCATTACCAGCGTCCAATTATCTAATCCCGGTCGACGGCAGCGGCCGATTTTGCTTCGCCTTCGCTGGAACTACCAGTGGGCTATCAATTATCGGCAACATCCAGCAGCAAGGATTTCGGGTGGTGTACGATTTGGCGAGTTCTCGGGTCGGATTTTCTCCTCGTGGTTGCGCCTAATTTCTTCGAACAGACAGCACGTAATGCGGGTTGCCGGAGAAATTCCTCCGCCGGCGTCTGTCCTAAAACCCTTCTTCCTCTTTTCCGACATGTAATAATAAAAAAGAAAGAAAAAAGAAAAAAAGGTGGAAGAAAAAGGGGTGCATAATTAGACTCCAACGGTGTCGTTTAACTTTTACTATTTTATTTAACTTTTTAGTTTTTTCATTCCAAATATCTCCATCTCTTTTCTTATTTAATTATATTTTCTATTCTATTGGTGTCGGTAAATTTATATATTAATTTCTCAAAAACATAAATATTTTTTTACTTTCTAAAATAAAGAGTCTTAATTGTTCAAGTT

Coding sequence (CDS)

ATGAAAATGGAGGCAAACACCATTTCACTTCCCTTCATCTTCTTCCTCCTTCTCGCTATTCTCTCTCTCTCCACCGCCTTCTCCGATTTCCAAACCCTTATTCTCAGATCCCTTCCCTCCTCACCTTCATTCTTACCCTCCGATTCCAACTCCTTCCTTTCCTCCGAGGCTACCGAAACGGAGCTTGGTTTAGAACTGCACCTTCACCATTTGGATGCTCTGTCTTTCAATCGAACGCCGGAGGAGCTCTTCCACCTCCGCCTTCAAAGAGACGCTATACGAGTCAAGAAGTTGAGTTCACTCGGTGCTACCTCTCGAAATCTGAGCCGACCCAGTGGGACCACCGGCTTCAGTAGCTCCGTCATCTCCGGACTCGCTCAAGGCAGTGGCGAGTACTTCACGCGCATCGGCGTCGGCACGCCACCCAAGTATGTCTACATGGTGCTTGACACCGGCAGTGATATTGTTTGGCTACAGTGTGCTCCTTGTAAGAATTGCTACTCTCAGACTGACCCTGTTTTCAACCCGGTTAAGTCTGGATCCTTTGCCAAGGTTCTATGCCGAACGCCGCTGTGCCGTCGGCTTGAATCTCCGGGGTGCAACCAGCGTCAGACGTGTCTTTACCAAGTTTCTTACGGCGACGGTTCCTATACCACCGGCGAGTTCGTCACCGAAACCCTAACCTTCCGCCGGACTAAAGTGGAGCAAGTAGCCCTTGGTTGTGGCCACGATAATGAGGGTTTATTCGTTGGTGCGGCTGGGCTTTTAGGTCTTGGCCGGGGAGGGTTGTCGTTTCCGTCCCAAGCTGGCCGGACTTTCAACCAAAAATTTTCTTACTGCTTGGTGGACCGTTCCGCCTCTTCCAAACCGTCCTCCGTAGTCTTCGGCAACTCCGCCGTCTCTCGAACCGCCCGGTTCACTCCTCTCCTCACGAACCCTAGGTTGGACACGTTTTACTACGTTGAACTACTCGGAATCAGCGTCGGAGGTACGCCCGTCTCCGGCATCTCCTCTTCACATTTCAAGCTTGATCGGACCGGTAACGGTGGAGTAATAATCGATTGCGGTACTTCTGTTACTCGATTGAACAAACCAGCGTACATTGCTCTGCGCGACGCCTTCCGTGCTGGAGCTTCAAGTTTGAAATCGGCGCCAGAGTTTTCTCTCTTCGATACTTGCTACGATCTGTCTGGGAAGACGACGGTGAAGGTCCCGACAGTGGTGCTACATTTCAGAGGTGCTGATGTATCATTACCAGCGTCCAATTATCTAATCCCGGTCGACGGCAGCGGCCGATTTTGCTTCGCCTTCGCTGGAACTACCAGTGGGCTATCAATTATCGGCAACATCCAGCAGCAAGGATTTCGGGTGGTGTACGATTTGGCGAGTTCTCGGGTCGGATTTTCTCCTCGTGGTTGCGCCTAA

Protein sequence

MKMEANTISLPFIFFLLLAILSLSTAFSDFQTLILRSLPSSPSFLPSDSNSFLSSEATETELGLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLSRPSGTTGFSSSVISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSGSFAKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAVSRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTSVTRLNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA
Homology
BLAST of MELO3C006020.jh1 vs. NCBI nr
Match: XP_008437888.1 (PREDICTED: aspartyl protease family protein 2 [Cucumis melo] >KAA0048886.1 protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucumis melo var. makuwa] >TYK20836.1 protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 932 bits (2409), Expect = 0.0
Identity = 474/474 (100.00%), Postives = 474/474 (100.00%), Query Frame = 0

Query: 1   MKMEANTISLPFIFFLLLAILSLSTAFSDFQTLILRSLPSSPSFLPSDSNSFLSSEATET 60
           MKMEANTISLPFIFFLLLAILSLSTAFSDFQTLILRSLPSSPSFLPSDSNSFLSSEATET
Sbjct: 1   MKMEANTISLPFIFFLLLAILSLSTAFSDFQTLILRSLPSSPSFLPSDSNSFLSSEATET 60

Query: 61  ELGLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLSRPSGTTGFSSS 120
           ELGLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLSRPSGTTGFSSS
Sbjct: 61  ELGLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLSRPSGTTGFSSS 120

Query: 121 VISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSG 180
           VISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSG
Sbjct: 121 VISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSG 180

Query: 181 SFAKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALG 240
           SFAKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALG
Sbjct: 181 SFAKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALG 240

Query: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAV 300
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAV
Sbjct: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAV 300

Query: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTSVT 360
           SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTSVT
Sbjct: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTSVT 360

Query: 361 RLNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420
           RLNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA
Sbjct: 361 RLNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420

Query: 421 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA 474
           SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA
Sbjct: 421 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA 474

BLAST of MELO3C006020.jh1 vs. NCBI nr
Match: XP_004133810.2 (aspartyl protease family protein 2 [Cucumis sativus] >KGN56421.1 hypothetical protein Csa_010568 [Cucumis sativus])

HSP 1 Score: 907 bits (2344), Expect = 0.0
Identity = 460/473 (97.25%), Postives = 467/473 (98.73%), Query Frame = 0

Query: 2   KMEANTISLPFIFFLLLAILSLSTAFSDFQTLILRSLPSSPSFLPSDSNSFLSSEATETE 61
           KME NTISLPFIFFLL  +LSL+TAFSDFQTL L SLPSSPSFLPSDSNSFLSSEAT++E
Sbjct: 41  KMEPNTISLPFIFFLL-TVLSLATAFSDFQTLPLTSLPSSPSFLPSDSNSFLSSEATQSE 100

Query: 62  LGLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLSRPSGTTGFSSSV 121
           LGLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLS+P GTTGFSSSV
Sbjct: 101 LGLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLSKPGGTTGFSSSV 160

Query: 122 ISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSGS 181
           ISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSGS
Sbjct: 161 ISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSGS 220

Query: 182 FAKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALGC 241
           FAKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALGC
Sbjct: 221 FAKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALGC 280

Query: 242 GHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAVS 301
           GHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAVS
Sbjct: 281 GHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAVS 340

Query: 302 RTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTSVTR 361
           RTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGI++SHFKLDRTGNGGVIIDCGTSVTR
Sbjct: 341 RTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGITASHFKLDRTGNGGVIIDCGTSVTR 400

Query: 362 LNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPAS 421
           LNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPAS
Sbjct: 401 LNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPAS 460

Query: 422 NYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA 474
           NYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA
Sbjct: 461 NYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA 512

BLAST of MELO3C006020.jh1 vs. NCBI nr
Match: XP_038877929.1 (aspartyl protease family protein 2-like [Benincasa hispida])

HSP 1 Score: 856 bits (2212), Expect = 6.34e-312
Identity = 438/472 (92.80%), Postives = 452/472 (95.76%), Query Frame = 0

Query: 3   MEANTISLPFIFFLLLAILSLSTAFSDFQTLILRSLPSSPSFLPSDSNSFLSSEATETEL 62
           MEA T    FIFFLL  +LSLSTA SDFQTLI  SLPSSPSFLPSDS SF+SS+ TE++L
Sbjct: 1   MEAMTKLFSFIFFLL-TLLSLSTAISDFQTLIPTSLPSSPSFLPSDSESFISSDTTESDL 60

Query: 63  GLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLSRPSGTTGFSSSVI 122
           GL LHLHHLDALS NRTPEELFHLRLQRDA+RVKKLSSL  +S+N+S+ SGT GFSSSVI
Sbjct: 61  GLTLHLHHLDALSLNRTPEELFHLRLQRDALRVKKLSSLHDSSQNVSQTSGT-GFSSSVI 120

Query: 123 SGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSGSF 182
           SGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSGSF
Sbjct: 121 SGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSGSF 180

Query: 183 AKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALGCG 242
           AKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVE+VALGCG
Sbjct: 181 AKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALGCG 240

Query: 243 HDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAVSR 302
           HDNEGLFVGAAGLLGLGRGGLSFPSQ GRTFNQKFSYCLVDRSASSKPSSVVFGN+AVSR
Sbjct: 241 HDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVFGNAAVSR 300

Query: 303 TARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTSVTRL 362
           TARFTPLLTNPRLDTFYYVELLGISVGGTPVSGIS+SHFKLD TGNGGVIIDCGTSVTRL
Sbjct: 301 TARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISASHFKLDPTGNGGVIIDCGTSVTRL 360

Query: 363 NKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPASN 422
           N+PAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPASN
Sbjct: 361 NRPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPASN 420

Query: 423 YLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA 474
           YLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA
Sbjct: 421 YLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA 470

BLAST of MELO3C006020.jh1 vs. NCBI nr
Match: XP_023527618.1 (aspartyl protease family protein 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 822 bits (2122), Expect = 1.04e-297
Identity = 425/476 (89.29%), Postives = 439/476 (92.23%), Query Frame = 0

Query: 3   MEANTISLPFIFFLLLAILSLSTAFSDFQTLILRSLPSSPSFLPSDSN----SFLSSEAT 62
           M A T   PFIFFLL  +LSLSTAFSDFQTL+ R LP+SPS L  +SN    SF SSEAT
Sbjct: 28  MVAKTSPFPFIFFLL-TLLSLSTAFSDFQTLVPRPLPTSPSSLAPESNQDSDSFFSSEAT 87

Query: 63  ETELGLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLSRPSGTTGFS 122
           E+E GL LHLHHLD+LS +RTPEELFHLRLQRDA+RV KLS L A S N+SR SGT GFS
Sbjct: 88  ESEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAGSPNVSRASGT-GFS 147

Query: 123 SSVISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVK 182
           SSVISGLAQGSGEYFTRIGVGTPP+YVYMVLDTGSDIVWLQCAPCKNCYSQTDPVF+PVK
Sbjct: 148 SSVISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVK 207

Query: 183 SGSFAKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVA 242
           SGSF+KVLCRTPLC RLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVE+VA
Sbjct: 208 SGSFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVA 267

Query: 243 LGCGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNS 302
           LGCGHDNEGLFVGAAGLLGLGRGGLSFPSQ GRTFNQKFSYCLVDRSASSKPS VVFGNS
Sbjct: 268 LGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSFVVFGNS 327

Query: 303 AVSRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTS 362
           AVSRTARFTPLLTNPRLDTFYYVELLGISVGG PVSGIS  HFKLD TGNGGVIIDCGTS
Sbjct: 328 AVSRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTS 387

Query: 363 VTRLNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSL 422
           VTRLN+PAYIALRDAFRAGASSLKSA EFSLFDTCYDLSGKTTVKVPTVVLHFR ADVSL
Sbjct: 388 VTRLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSL 447

Query: 423 PASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA 474
           PASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLA SRVGFSPRGCA
Sbjct: 448 PASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 501

BLAST of MELO3C006020.jh1 vs. NCBI nr
Match: XP_022924595.1 (aspartyl protease family protein 2-like [Cucurbita moschata] >KAG6582675.1 Aspartyl protease family protein 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019074.1 Aspartyl protease family protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 820 bits (2117), Expect = 2.19e-297
Identity = 424/476 (89.08%), Postives = 439/476 (92.23%), Query Frame = 0

Query: 3   MEANTISLPFIFFLLLAILSLSTAFSDFQTLILRSLPSSPSFLPSDSN----SFLSSEAT 62
           M A T    FIF +LL +LSLSTAFSDFQTL+ R LP+SPS L  +SN    SF SSEAT
Sbjct: 1   MVAKTSPFTFIF-VLLTLLSLSTAFSDFQTLVPRPLPTSPSSLAPESNEDSDSFFSSEAT 60

Query: 63  ETELGLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLSRPSGTTGFS 122
           E+E GL LHLHHLD+LS +RTPEELFHLRLQRDA+RV KLS L A S N+SR SGT GFS
Sbjct: 61  ESEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAVSPNVSRASGT-GFS 120

Query: 123 SSVISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVK 182
           SSVISGLAQGSGEYFTRIGVGTPP+YVYMVLDTGSDIVWLQCAPCKNCYSQTDPVF+PVK
Sbjct: 121 SSVISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVK 180

Query: 183 SGSFAKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVA 242
           SGSF+KVLCRTPLC RLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVE+VA
Sbjct: 181 SGSFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVA 240

Query: 243 LGCGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNS 302
           LGCGHDNEGLFVGAAGLLGLGRGGLSFPSQ GRTFNQKFSYCLVDRSASSKPSSVVFGNS
Sbjct: 241 LGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVFGNS 300

Query: 303 AVSRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTS 362
           AVSRTARFTPLLTNPRLDTFYYVELLGISVGG PVSGIS  HFKLD TGNGGVIIDCGTS
Sbjct: 301 AVSRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTS 360

Query: 363 VTRLNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSL 422
           VTRLN+PAYIALRDAFRAGASSLKSA EFSLFDTCYDLSGKTTVKVPTVVLHFR ADVSL
Sbjct: 361 VTRLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSL 420

Query: 423 PASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA 474
           PASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLA SRVGFSPRGCA
Sbjct: 421 PASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474

BLAST of MELO3C006020.jh1 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 6.4e-183
Identity = 338/486 (69.55%), Postives = 391/486 (80.45%), Query Frame = 0

Query: 2   KMEANTISLPFIFFLLLAILSLSTAFSDFQTLI--LRSLP--SSPSFLP-SDSNSFLSSE 61
           + +A   SL F F  L +  SL +    FQTL     SLP  S  SF P SDS S L SE
Sbjct: 4   RRKALLFSLCFFFLSLPSFSSLPS----FQTLFPNSHSLPCASPVSFQPDSDSESLLESE 63

Query: 62  -----ATETELGLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGA--TSRNLS 121
                 +E+   + L+L H+DALS N+TP+ELF  RLQRD+ RVK +++L A    RN++
Sbjct: 64  FESGSDSESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVT 123

Query: 122 RPSGTTGFSSSVISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQ 181
                 GFSSSV+SGL+QGSGEYFTR+GVGTP +YVYMVLDTGSDIVWLQCAPC+ CYSQ
Sbjct: 124 HAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQ 183

Query: 182 TDPVFNPVKSGSFAKVLCRTPLCRRLESPGCN-QRQTCLYQVSYGDGSYTTGEFVTETLT 241
           +DP+F+P KS ++A + C +P CRRL+S GCN +R+TCLYQVSYGDGS+T G+F TETLT
Sbjct: 184 SDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLT 243

Query: 242 FRRTKVEQVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASS 301
           FRR +V+ VALGCGHDNEGLFVGAAGLLGLG+G LSFP Q G  FNQKFSYCLVDRSASS
Sbjct: 244 FRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASS 303

Query: 302 KPSSVVFGNSAVSRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISSSHFKLDRTGN 361
           KPSSVVFGN+AVSR ARFTPLL+NP+LDTFYYV LLGISVGGT V G+++S FKLD+ GN
Sbjct: 304 KPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGN 363

Query: 362 GGVIIDCGTSVTRLNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVV 421
           GGVIID GTSVTRL +PAYIA+RDAFR GA +LK AP+FSLFDTC+DLS    VKVPTVV
Sbjct: 364 GGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVV 423

Query: 422 LHFRGADVSLPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGF 475
           LHFRGADVSLPA+NYLIPVD +G+FCFAFAGT  GLSIIGNIQQQGFRVVYDLASSRVGF
Sbjct: 424 LHFRGADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGF 483

BLAST of MELO3C006020.jh1 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 1.2e-109
Identity = 227/461 (49.24%), Postives = 287/461 (62.26%), Query Frame = 0

Query: 31  QTLILRSLPSSPSFLPSDSNSFLSSEA---TETELGLELHLHHLDALSFNRTPEELFHLR 90
           QT  + SL  + S L +     LS      + + L LELH       S ++  + L   R
Sbjct: 46  QTQTILSLDPTRSSLTTTKPESLSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSR 105

Query: 91  LQRDAIRV-----KKLSSLGATSRNLSRP-------SGTTGFSSSVISGLAQGSGEYFTR 150
           L+RD+ RV     K   ++    R+  +P         T   ++ V+SG +QGSGEYF+R
Sbjct: 106 LERDSSRVAGIVAKIRFAVEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSR 165

Query: 151 IGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSGSFAKVLCRTPLCRRL 210
           IGVGTP K +Y+VLDTGSD+ W+QC PC +CY Q+DPVFNP  S ++  + C  P C  L
Sbjct: 166 IGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLL 225

Query: 211 ESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRT-KVEQVALGCGHDNEGLFVGAAG 270
           E+  C   + CLYQVSYGDGS+T GE  T+T+TF  + K+  VALGCGHDNEGLF GAAG
Sbjct: 226 ETSACRSNK-CLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAG 285

Query: 271 LLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAVSRTARFTPLLTNPR 330
           LLGLG G LS  +Q   T    FSYCLVDR  S K SS+ F +  +       PLL N +
Sbjct: 286 LLGLGGGVLSITNQMKAT---SFSYCLVDRD-SGKSSSLDFNSVQLGGGDATAPLLRNKK 345

Query: 331 LDTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTSVTRLNKPAYIALRDAF 390
           +DTFYYV L G SVGG  V  +  + F +D +G+GGVI+DCGT+VTRL   AY +LRDAF
Sbjct: 346 IDTFYYVGLSGFSVGGEKVV-LPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAF 405

Query: 391 -RAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGA-DVSLPASNYLIPVDGSGR 450
            +   +  K +   SLFDTCYD S  +TVKVPTV  HF G   + LPA NYLIPVD SG 
Sbjct: 406 LKLTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGT 465

Query: 451 FCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGC 474
           FCFAFA T+S LSIIGN+QQQG R+ YDL+ + +G S   C
Sbjct: 466 FCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of MELO3C006020.jh1 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 3.6e-109
Identity = 218/472 (46.19%), Postives = 293/472 (62.08%), Query Frame = 0

Query: 10  LPFIFFLLLAILSLST----AFSDFQTL-ILRSLPSSPSFLPSDSNSFLSSEATETELGL 69
           LP  FF L   L LS+    +F DFQ + +L+   +  + LP  +N+  S E++      
Sbjct: 3   LPLFFFFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTATLPDFNNTHFSDESSSKYTLR 62

Query: 70  ELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKL--SSLGATSRNLSRPSGTTGFSSSVI 129
            LH     ++++ R      H R++RD  RV  +     G    +         F S ++
Sbjct: 63  LLHRDRFPSVTY-RNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYEVNDFGSDIV 122

Query: 130 SGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSGSF 189
           SG+ QGSGEYF RIGVG+PP+  YMV+D+GSD+VW+QC PCK CY Q+DPVF+P KSGS+
Sbjct: 123 SGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGSY 182

Query: 190 AKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALGCG 249
             V C + +C R+E+ GC+    C Y+V YGDGSYT G    ETLTF +T V  VA+GCG
Sbjct: 183 TGVSCGSSVCDRIENSGCHS-GGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMGCG 242

Query: 250 HDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAVSR 309
           H N G+F+GAAGLLG+G G +SF  Q        F YCLV R   S   S+VFG  A+  
Sbjct: 243 HRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDS-TGSLVFGREALPV 302

Query: 310 TARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTSVTRL 369
            A + PL+ NPR  +FYYV L G+ VGG  +  +    F L  TG+GGV++D GT+VTRL
Sbjct: 303 GASWVPLVRNPRAPSFYYVGLKGLGVGGVRIP-LPDGVFDLTETGDGGVVMDTGTAVTRL 362

Query: 370 NKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHF-RGADVSLPAS 429
              AY+A RD F++  ++L  A   S+FDTCYDLSG  +V+VPTV  +F  G  ++LPA 
Sbjct: 363 PTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPAR 422

Query: 430 NYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGC 474
           N+L+PVD SG +CFAFA + +GLSIIGNIQQ+G +V +D A+  VGF P  C
Sbjct: 423 NFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

BLAST of MELO3C006020.jh1 vs. ExPASy Swiss-Prot
Match: Q9LEW3 (Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 7.5e-75
Identity = 175/437 (40.05%), Postives = 242/437 (55.38%), Query Frame = 0

Query: 43  SFLPSDSNSFLSSEATETELGLE-LHLHHLDALSFNRTPEELFHLR-LQRDAIRVKKL-S 102
           S  PS S+   SS+A+ T+  L  +H+H   A S   +   + H   ++RD  RV+ + S
Sbjct: 44  SLFPSSSSCVPSSKASNTKSSLRVVHMH--GACSHLSSDARVDHDEIIRRDQARVESIYS 103

Query: 103 SLGATSRNLSRPSGTTGFSSSVISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQ 162
            L   S N    + +T   +   SG+  GSG Y   IG+GTP   + +V DTGSD+ W Q
Sbjct: 104 KLSKNSANEVSEAKSTELPAK--SGITLGSGNYIVTIGIGTPKHDLSLVFDTGSDLTWTQ 163

Query: 163 CAPC-KNCYSQTDPVFNPVKSGSFAKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYT 222
           C PC  +CYSQ +P FNP  S ++  V C +P+C   ES  C+    C+Y + YGD S+T
Sbjct: 164 CEPCLGSCYSQKEPKFNPSSSSTYQNVSCSSPMCEDAES--CS-ASNCVYSIVYGDKSFT 223

Query: 223 TGEFVTETLTFRRTKV-EQVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKF 282
            G    E  T   + V E V  GCG +N+GLF G AGLLGLG G LS P+Q   T+N  F
Sbjct: 224 QGFLAKEKFTLTNSDVLEDVYFGCGENNQGLFDGVAGLLGLGPGKLSLPAQTTTTYNNIF 283

Query: 283 SYCLVDRSASSKPSSVVFGNSAVSRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGIS 342
           SYCL   +++S    + FG++ +S + +FTP+ + P     Y ++++GISVG   ++ I+
Sbjct: 284 SYCLPSFTSNS-TGHLTFGSAGISESVKFTPISSFPSAFN-YGIDIIGISVGDKELA-IT 343

Query: 343 SSHFKLDRTGNGGVIIDCGTSVTRLNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLS 402
            + F  +     G IID GT  TRL    Y  LR  F+   SS KS   + LFDTCYD +
Sbjct: 344 PNSFSTE-----GAIIDSGTVFTRLPTKVYAELRSVFKEKMSSYKSTSGYGLFDTCYDFT 403

Query: 403 GKTTVKVPTVVLHFRGAD-VSLPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFR 462
           G  TV  PT+   F G+  V L  S   +P+  S + C AFAG     +I GN+QQ    
Sbjct: 404 GLDTVTYPTIAFSFAGSTVVELDGSGISLPIKIS-QVCLAFAGNDDLPAIFGNVQQTTLD 463

Query: 463 VVYDLASSRVGFSPRGC 474
           VVYD+A  RVGF+P GC
Sbjct: 464 VVYDVAGGRVGFAPNGC 464

BLAST of MELO3C006020.jh1 vs. ExPASy Swiss-Prot
Match: Q8S9J6 (Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At5g10770 PE=2 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 3.2e-73
Identity = 172/445 (38.65%), Postives = 234/445 (52.58%), Query Frame = 0

Query: 43  SFLPSDSNS-FLSSEATETELGLELHLHHLDALSFNR----TPEELFHLRLQRDAIRVKK 102
           S LPS S+S  LS  A+ T+  L +   H      N     +P+ +  LRL  D  RV  
Sbjct: 40  SLLPSSSSSCVLSPRASTTKSSLHVTHRHGTCSRLNNGKATSPDHVEILRL--DQARVNS 99

Query: 103 LSSLGATSRNLSRPSGTTGFSSSVISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVW 162
           + S  +        S +         G   GSG Y   +G+GTP   + ++ DTGSD+ W
Sbjct: 100 IHSKLSKKLATDHVSESKSTDLPAKDGSTLGSGNYIVTVGLGTPKNDLSLIFDTGSDLTW 159

Query: 163 LQCAPC-KNCYSQTDPVFNPVKSGSFAKVLCRTPLCRRLES----PGCNQRQTCLYQVSY 222
            QC PC + CY Q +P+FNP KS S+  V C +  C  L S     G      C+Y + Y
Sbjct: 160 TQCQPCVRTCYDQKEPIFNPSKSTSYYNVSCSSAACGSLSSATGNAGSCSASNCIYGIQY 219

Query: 223 GDGSYTTGEFVTETLTFRRTKV-EQVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQAGR 282
           GD S++ G    E  T   + V + V  GCG +N+GLF G AGLLGLGR  LSFPSQ   
Sbjct: 220 GDQSFSVGFLAKEKFTLTNSDVFDGVYFGCGENNQGLFTGVAGLLGLGRDKLSFPSQTAT 279

Query: 283 TFNQKFSYCLVDRSASSKPSSVVFGNSAVSRTARFTPLLTNPRLDTFYYVELLGISVGGT 342
            +N+ FSYCL   S++S    + FG++ +SR+ +FTP+ T     +FY + ++ I+VGG 
Sbjct: 280 AYNKIFSYCL--PSSASYTGHLTFGSAGISRSVKFTPISTITDGTSFYGLNIVAITVGGQ 339

Query: 343 PVSGISSSHFKLDRTGNGGVIIDCGTSVTRLNKPAYIALRDAFRAGASSLKSAPEFSLFD 402
            +  I S+ F        G +ID GT +TRL   AY ALR +F+A  S   +    S+ D
Sbjct: 340 KLP-IPSTVF-----STPGALIDSGTVITRLPPKAYAALRSSFKAKMSKYPTTSGVSILD 399

Query: 403 TCYDLSGKTTVKVPTVVLHFRGADVSLPASNYLIPVDGSGRFCFAFAGTT--SGLSIIGN 462
           TC+DLSG  TV +P V   F G  V    S  +  V    + C AFAG +  S  +I GN
Sbjct: 400 TCFDLSGFKTVTIPKVAFSFSGGAVVELGSKGIFYVFKISQVCLAFAGNSDDSNAAIFGN 459

Query: 463 IQQQGFRVVYDLASSRVGFSPRGCA 475
           +QQQ   VVYD A  RVGF+P GC+
Sbjct: 460 VQQQTLEVVYDGAGGRVGFAPNGCS 474

BLAST of MELO3C006020.jh1 vs. ExPASy TrEMBL
Match: A0A5A7U3R3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold291G00990 PE=3 SV=1)

HSP 1 Score: 932 bits (2409), Expect = 0.0
Identity = 474/474 (100.00%), Postives = 474/474 (100.00%), Query Frame = 0

Query: 1   MKMEANTISLPFIFFLLLAILSLSTAFSDFQTLILRSLPSSPSFLPSDSNSFLSSEATET 60
           MKMEANTISLPFIFFLLLAILSLSTAFSDFQTLILRSLPSSPSFLPSDSNSFLSSEATET
Sbjct: 1   MKMEANTISLPFIFFLLLAILSLSTAFSDFQTLILRSLPSSPSFLPSDSNSFLSSEATET 60

Query: 61  ELGLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLSRPSGTTGFSSS 120
           ELGLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLSRPSGTTGFSSS
Sbjct: 61  ELGLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLSRPSGTTGFSSS 120

Query: 121 VISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSG 180
           VISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSG
Sbjct: 121 VISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSG 180

Query: 181 SFAKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALG 240
           SFAKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALG
Sbjct: 181 SFAKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALG 240

Query: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAV 300
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAV
Sbjct: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAV 300

Query: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTSVT 360
           SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTSVT
Sbjct: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTSVT 360

Query: 361 RLNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420
           RLNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA
Sbjct: 361 RLNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420

Query: 421 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA 474
           SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA
Sbjct: 421 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA 474

BLAST of MELO3C006020.jh1 vs. ExPASy TrEMBL
Match: A0A1S3AV66 (aspartyl protease family protein 2 OS=Cucumis melo OX=3656 GN=LOC103483183 PE=3 SV=1)

HSP 1 Score: 932 bits (2409), Expect = 0.0
Identity = 474/474 (100.00%), Postives = 474/474 (100.00%), Query Frame = 0

Query: 1   MKMEANTISLPFIFFLLLAILSLSTAFSDFQTLILRSLPSSPSFLPSDSNSFLSSEATET 60
           MKMEANTISLPFIFFLLLAILSLSTAFSDFQTLILRSLPSSPSFLPSDSNSFLSSEATET
Sbjct: 1   MKMEANTISLPFIFFLLLAILSLSTAFSDFQTLILRSLPSSPSFLPSDSNSFLSSEATET 60

Query: 61  ELGLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLSRPSGTTGFSSS 120
           ELGLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLSRPSGTTGFSSS
Sbjct: 61  ELGLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLSRPSGTTGFSSS 120

Query: 121 VISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSG 180
           VISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSG
Sbjct: 121 VISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSG 180

Query: 181 SFAKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALG 240
           SFAKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALG
Sbjct: 181 SFAKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALG 240

Query: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAV 300
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAV
Sbjct: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAV 300

Query: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTSVT 360
           SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTSVT
Sbjct: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTSVT 360

Query: 361 RLNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420
           RLNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA
Sbjct: 361 RLNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420

Query: 421 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA 474
           SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA
Sbjct: 421 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA 474

BLAST of MELO3C006020.jh1 vs. ExPASy TrEMBL
Match: A0A0A0L8K0 (Aspartic proteinase nepenthesin-1 OS=Cucumis sativus OX=3659 GN=Csa_3G119540 PE=3 SV=1)

HSP 1 Score: 907 bits (2344), Expect = 0.0
Identity = 460/473 (97.25%), Postives = 467/473 (98.73%), Query Frame = 0

Query: 2   KMEANTISLPFIFFLLLAILSLSTAFSDFQTLILRSLPSSPSFLPSDSNSFLSSEATETE 61
           KME NTISLPFIFFLL  +LSL+TAFSDFQTL L SLPSSPSFLPSDSNSFLSSEAT++E
Sbjct: 41  KMEPNTISLPFIFFLL-TVLSLATAFSDFQTLPLTSLPSSPSFLPSDSNSFLSSEATQSE 100

Query: 62  LGLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLSRPSGTTGFSSSV 121
           LGLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLS+P GTTGFSSSV
Sbjct: 101 LGLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLSKPGGTTGFSSSV 160

Query: 122 ISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSGS 181
           ISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSGS
Sbjct: 161 ISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSGS 220

Query: 182 FAKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALGC 241
           FAKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALGC
Sbjct: 221 FAKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALGC 280

Query: 242 GHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAVS 301
           GHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAVS
Sbjct: 281 GHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAVS 340

Query: 302 RTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTSVTR 361
           RTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGI++SHFKLDRTGNGGVIIDCGTSVTR
Sbjct: 341 RTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGITASHFKLDRTGNGGVIIDCGTSVTR 400

Query: 362 LNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPAS 421
           LNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPAS
Sbjct: 401 LNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPAS 460

Query: 422 NYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA 474
           NYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA
Sbjct: 461 NYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA 512

BLAST of MELO3C006020.jh1 vs. ExPASy TrEMBL
Match: A0A6J1EFG4 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111432036 PE=3 SV=1)

HSP 1 Score: 820 bits (2117), Expect = 1.06e-297
Identity = 424/476 (89.08%), Postives = 439/476 (92.23%), Query Frame = 0

Query: 3   MEANTISLPFIFFLLLAILSLSTAFSDFQTLILRSLPSSPSFLPSDSN----SFLSSEAT 62
           M A T    FIF +LL +LSLSTAFSDFQTL+ R LP+SPS L  +SN    SF SSEAT
Sbjct: 1   MVAKTSPFTFIF-VLLTLLSLSTAFSDFQTLVPRPLPTSPSSLAPESNEDSDSFFSSEAT 60

Query: 63  ETELGLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLSRPSGTTGFS 122
           E+E GL LHLHHLD+LS +RTPEELFHLRLQRDA+RV KLS L A S N+SR SGT GFS
Sbjct: 61  ESEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAVSPNVSRASGT-GFS 120

Query: 123 SSVISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVK 182
           SSVISGLAQGSGEYFTRIGVGTPP+YVYMVLDTGSDIVWLQCAPCKNCYSQTDPVF+PVK
Sbjct: 121 SSVISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVK 180

Query: 183 SGSFAKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVA 242
           SGSF+KVLCRTPLC RLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVE+VA
Sbjct: 181 SGSFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVA 240

Query: 243 LGCGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNS 302
           LGCGHDNEGLFVGAAGLLGLGRGGLSFPSQ GRTFNQKFSYCLVDRSASSKPSSVVFGNS
Sbjct: 241 LGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVFGNS 300

Query: 303 AVSRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTS 362
           AVSRTARFTPLLTNPRLDTFYYVELLGISVGG PVSGIS  HFKLD TGNGGVIIDCGTS
Sbjct: 301 AVSRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTS 360

Query: 363 VTRLNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSL 422
           VTRLN+PAYIALRDAFRAGASSLKSA EFSLFDTCYDLSGKTTVKVPTVVLHFR ADVSL
Sbjct: 361 VTRLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSL 420

Query: 423 PASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA 474
           PASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLA SRVGFSPRGCA
Sbjct: 421 PASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474

BLAST of MELO3C006020.jh1 vs. ExPASy TrEMBL
Match: A0A6J1IY26 (aspartyl protease family protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111479557 PE=3 SV=1)

HSP 1 Score: 819 bits (2116), Expect = 1.45e-297
Identity = 422/475 (88.84%), Postives = 438/475 (92.21%), Query Frame = 0

Query: 3   MEANTISLPFIFFLLLAILSLSTAFSDFQTLILRSLPSSPSFLPSDS---NSFLSSEATE 62
           M A T   PFIFFLL  +L LSTAFSDFQTL+ R LP+SPSFL  +S   +   SSEATE
Sbjct: 1   MVAKTSPFPFIFFLL-TLLPLSTAFSDFQTLVPRPLPTSPSFLAPESTEGSDSFSSEATE 60

Query: 63  TELGLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLSRPSGTTGFSS 122
           +E GL LHLHHLD+LS +RTPEELFHLRLQRDA+RV KLS L A SRN+SR SGT GFSS
Sbjct: 61  SEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAASRNVSRASGT-GFSS 120

Query: 123 SVISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKS 182
           SVISGLAQGSGEYFTRIGVGTPP+YVY+VLDTGSDIVWLQCAPCKNCYSQTDPVF+PVKS
Sbjct: 121 SVISGLAQGSGEYFTRIGVGTPPRYVYLVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKS 180

Query: 183 GSFAKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVAL 242
           GSF+KVLCRTPLC RLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVE+VAL
Sbjct: 181 GSFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVAL 240

Query: 243 GCGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSA 302
           GCGHDNEGLFVGAAGLLGLGRGGLSFPSQ GR FNQKFSYCLVDRSASSKPSSVVFGNSA
Sbjct: 241 GCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRAFNQKFSYCLVDRSASSKPSSVVFGNSA 300

Query: 303 VSRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTSV 362
           VSRTARFTPLLTNPRLDTFYYVELLGISVGG PVSGIS  HFKLD TGNGGVIIDCGTSV
Sbjct: 301 VSRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSV 360

Query: 363 TRLNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLP 422
           TRLN+PAYIALRDAFRAGASSLKSA EFSLFDTCYDLSGKTTVKVPTVVLHFR ADVSLP
Sbjct: 361 TRLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLP 420

Query: 423 ASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA 474
           ASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLA SRVGFSPRGCA
Sbjct: 421 ASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 473

BLAST of MELO3C006020.jh1 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 641.7 bits (1654), Expect = 4.5e-184
Identity = 338/486 (69.55%), Postives = 391/486 (80.45%), Query Frame = 0

Query: 2   KMEANTISLPFIFFLLLAILSLSTAFSDFQTLI--LRSLP--SSPSFLP-SDSNSFLSSE 61
           + +A   SL F F  L +  SL +    FQTL     SLP  S  SF P SDS S L SE
Sbjct: 4   RRKALLFSLCFFFLSLPSFSSLPS----FQTLFPNSHSLPCASPVSFQPDSDSESLLESE 63

Query: 62  -----ATETELGLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGA--TSRNLS 121
                 +E+   + L+L H+DALS N+TP+ELF  RLQRD+ RVK +++L A    RN++
Sbjct: 64  FESGSDSESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVT 123

Query: 122 RPSGTTGFSSSVISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQ 181
                 GFSSSV+SGL+QGSGEYFTR+GVGTP +YVYMVLDTGSDIVWLQCAPC+ CYSQ
Sbjct: 124 HAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQ 183

Query: 182 TDPVFNPVKSGSFAKVLCRTPLCRRLESPGCN-QRQTCLYQVSYGDGSYTTGEFVTETLT 241
           +DP+F+P KS ++A + C +P CRRL+S GCN +R+TCLYQVSYGDGS+T G+F TETLT
Sbjct: 184 SDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLT 243

Query: 242 FRRTKVEQVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASS 301
           FRR +V+ VALGCGHDNEGLFVGAAGLLGLG+G LSFP Q G  FNQKFSYCLVDRSASS
Sbjct: 244 FRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASS 303

Query: 302 KPSSVVFGNSAVSRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISSSHFKLDRTGN 361
           KPSSVVFGN+AVSR ARFTPLL+NP+LDTFYYV LLGISVGGT V G+++S FKLD+ GN
Sbjct: 304 KPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGN 363

Query: 362 GGVIIDCGTSVTRLNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVV 421
           GGVIID GTSVTRL +PAYIA+RDAFR GA +LK AP+FSLFDTC+DLS    VKVPTVV
Sbjct: 364 GGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVV 423

Query: 422 LHFRGADVSLPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGF 475
           LHFRGADVSLPA+NYLIPVD +G+FCFAFAGT  GLSIIGNIQQQGFRVVYDLASSRVGF
Sbjct: 424 LHFRGADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGF 483

BLAST of MELO3C006020.jh1 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 588.6 bits (1516), Expect = 4.6e-168
Identity = 305/473 (64.48%), Postives = 363/473 (76.74%), Query Frame = 0

Query: 14  FFLLLAILSLSTAFSDFQTLILRSLPSSPSFLPSDSNSFLSSEATETELGLELHLHHLDA 73
           F +   +   S+A S +QTL++ +LPSS +    +S S      +E+   L +HL H+DA
Sbjct: 11  FSVFAVLFFTSSASSQYQTLVVNTLPSSATLSWPESESLTDESLSESTTSLSVHLSHVDA 70

Query: 74  LS--FNRTPEELFHLRLQRDAIRVKKLSSLGA--TSRNLSR--PSGTTGFSSSVISGLAQ 133
           LS   + +P +LF+LRLQRD++RVK ++SL A  T RN ++  P    GFS +VISGL+Q
Sbjct: 71  LSSFSDASPADLFNLRLQRDSLRVKSITSLAAVSTGRNATKRTPRTAGGFSGAVISGLSQ 130

Query: 134 GSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSGSFAKVLC 193
           GSGEYF R+GVGTP   VYMVLDTGSD+VWLQC+PCK CY+QTD +F+P KS +FA V C
Sbjct: 131 GSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCKACYNQTDAIFDPKKSKTFATVPC 190

Query: 194 RTPLCRRL-ESPGCNQR--QTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALGCGHD 253
            + LCRRL +S  C  R  +TCLYQVSYGDGS+T G+F TETLTF   +V+ V LGCGHD
Sbjct: 191 GSRLCRRLDDSSECVTRRSKTCLYQVSYGDGSFTEGDFSTETLTFHGARVDHVPLGCGHD 250

Query: 254 NEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDR----SASSKPSSVVFGNSAV 313
           NEGLFVGAAGLLGLGRGGLSFPSQ    +N KFSYCLVDR    S+S  PS++VFGN+AV
Sbjct: 251 NEGLFVGAAGLLGLGRGGLSFPSQTKNRYNGKFSYCLVDRTSSGSSSKPPSTIVFGNAAV 310

Query: 314 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTSVT 373
            +T+ FTPLLTNP+LDTFYY++LLGISVGG+ V G+S S FKLD TGNGGVIID GTSVT
Sbjct: 311 PKTSVFTPLLTNPKLDTFYYLQLLGISVGGSRVPGVSESQFKLDATGNGGVIIDSGTSVT 370

Query: 374 RLNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 433
           RL +PAY+ALRDAFR GA+ LK AP +SLFDTC+DLSG TTVKVPTVV HF G +VSLPA
Sbjct: 371 RLTQPAYVALRDAFRLGATKLKRAPSYSLFDTCFDLSGMTTVKVPTVVFHFGGGEVSLPA 430

Query: 434 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGC 474
           SNYLIPV+  GRFCFAFAGT   LSIIGNIQQQGFRV YDL  SRVGF  R C
Sbjct: 431 SNYLIPVNTEGRFCFAFAGTMGSLSIIGNIQQQGFRVAYDLVGSRVGFLSRAC 483

BLAST of MELO3C006020.jh1 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 406.8 bits (1044), Expect = 2.5e-113
Identity = 220/399 (55.14%), Postives = 267/399 (66.92%), Query Frame = 0

Query: 87  RLQRDAIRVKKL-SSLGATSRNLS----RPSGT------TGFSSSVISGLAQGSGEYFTR 146
           RL RD  RVK L + L     N+S    +P  T          + +ISG  QGSGEYFTR
Sbjct: 92  RLNRDTARVKSLITRLDLAINNISKADLKPISTMYTTEEQDIEAPLISGTTQGSGEYFTR 151

Query: 147 IGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSGSFAKVLCRTPLCRRL 206
           +G+G P + VYMVLDTGSD+ WLQC PC +CY QT+P+F P  S S+  + C TP C  L
Sbjct: 152 VGIGKPAREVYMVLDTGSDVNWLQCTPCADCYHQTEPIFEPSSSSSYEPLSCDTPQCNAL 211

Query: 207 ESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALGCGHDNEGLFVGAAGL 266
           E   C +  TCLY+VSYGDGSYT G+F TETLT   T V+ VA+GCGH NEGLFVGAAGL
Sbjct: 212 EVSEC-RNATCLYEVSYGDGSYTVGDFATETLTIGSTLVQNVAVGCGHSNEGLFVGAAGL 271

Query: 267 LGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAVSRTARFTPLLTNPRL 326
           LGLG G L+ PSQ   T    FSYCLVDR + S  S+V FG S +S  A   PLL N +L
Sbjct: 272 LGLGGGLLALPSQLNTT---SFSYCLVDRDSDS-ASTVDFGTS-LSPDAVVAPLLRNHQL 331

Query: 327 DTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTSVTRLNKPAYIALRDAFR 386
           DTFYY+ L GISVGG  +  I  S F++D +G+GG+IID GT+VTRL    Y +LRD+F 
Sbjct: 332 DTFYYLGLTGISVGGELLQ-IPQSSFEMDESGSGGIIIDSGTAVTRLQTEIYNSLRDSFV 391

Query: 387 AGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGAD-VSLPASNYLIPVDGSGRFC 446
            G   L+ A   ++FDTCY+LS KTTV+VPTV  HF G   ++LPA NY+IPVD  G FC
Sbjct: 392 KGTLDLEKAAGVAMFDTCYNLSAKTTVEVPTVAFHFPGGKMLALPAKNYMIPVDSVGTFC 451

Query: 447 FAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGC 474
            AFA T S L+IIGN+QQQG RV +DLA+S +GFS   C
Sbjct: 452 LAFAPTASSLAIIGNVQQQGTRVTFDLANSLIGFSSNKC 483

BLAST of MELO3C006020.jh1 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 398.3 bits (1022), Expect = 8.7e-111
Identity = 227/461 (49.24%), Postives = 287/461 (62.26%), Query Frame = 0

Query: 31  QTLILRSLPSSPSFLPSDSNSFLSSEA---TETELGLELHLHHLDALSFNRTPEELFHLR 90
           QT  + SL  + S L +     LS      + + L LELH       S ++  + L   R
Sbjct: 46  QTQTILSLDPTRSSLTTTKPESLSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSR 105

Query: 91  LQRDAIRV-----KKLSSLGATSRNLSRP-------SGTTGFSSSVISGLAQGSGEYFTR 150
           L+RD+ RV     K   ++    R+  +P         T   ++ V+SG +QGSGEYF+R
Sbjct: 106 LERDSSRVAGIVAKIRFAVEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSR 165

Query: 151 IGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSGSFAKVLCRTPLCRRL 210
           IGVGTP K +Y+VLDTGSD+ W+QC PC +CY Q+DPVFNP  S ++  + C  P C  L
Sbjct: 166 IGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLL 225

Query: 211 ESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRT-KVEQVALGCGHDNEGLFVGAAG 270
           E+  C   + CLYQVSYGDGS+T GE  T+T+TF  + K+  VALGCGHDNEGLF GAAG
Sbjct: 226 ETSACRSNK-CLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAG 285

Query: 271 LLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAVSRTARFTPLLTNPR 330
           LLGLG G LS  +Q   T    FSYCLVDR  S K SS+ F +  +       PLL N +
Sbjct: 286 LLGLGGGVLSITNQMKAT---SFSYCLVDRD-SGKSSSLDFNSVQLGGGDATAPLLRNKK 345

Query: 331 LDTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTSVTRLNKPAYIALRDAF 390
           +DTFYYV L G SVGG  V  +  + F +D +G+GGVI+DCGT+VTRL   AY +LRDAF
Sbjct: 346 IDTFYYVGLSGFSVGGEKVV-LPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAF 405

Query: 391 -RAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGA-DVSLPASNYLIPVDGSGR 450
            +   +  K +   SLFDTCYD S  +TVKVPTV  HF G   + LPA NYLIPVD SG 
Sbjct: 406 LKLTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGT 465

Query: 451 FCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGC 474
           FCFAFA T+S LSIIGN+QQQG R+ YDL+ + +G S   C
Sbjct: 466 FCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of MELO3C006020.jh1 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 396.7 bits (1018), Expect = 2.5e-110
Identity = 218/472 (46.19%), Postives = 293/472 (62.08%), Query Frame = 0

Query: 10  LPFIFFLLLAILSLST----AFSDFQTL-ILRSLPSSPSFLPSDSNSFLSSEATETELGL 69
           LP  FF L   L LS+    +F DFQ + +L+   +  + LP  +N+  S E++      
Sbjct: 3   LPLFFFFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTATLPDFNNTHFSDESSSKYTLR 62

Query: 70  ELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKL--SSLGATSRNLSRPSGTTGFSSSVI 129
            LH     ++++ R      H R++RD  RV  +     G    +         F S ++
Sbjct: 63  LLHRDRFPSVTY-RNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYEVNDFGSDIV 122

Query: 130 SGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSGSF 189
           SG+ QGSGEYF RIGVG+PP+  YMV+D+GSD+VW+QC PCK CY Q+DPVF+P KSGS+
Sbjct: 123 SGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGSY 182

Query: 190 AKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALGCG 249
             V C + +C R+E+ GC+    C Y+V YGDGSYT G    ETLTF +T V  VA+GCG
Sbjct: 183 TGVSCGSSVCDRIENSGCHS-GGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMGCG 242

Query: 250 HDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAVSR 309
           H N G+F+GAAGLLG+G G +SF  Q        F YCLV R   S   S+VFG  A+  
Sbjct: 243 HRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDS-TGSLVFGREALPV 302

Query: 310 TARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTSVTRL 369
            A + PL+ NPR  +FYYV L G+ VGG  +  +    F L  TG+GGV++D GT+VTRL
Sbjct: 303 GASWVPLVRNPRAPSFYYVGLKGLGVGGVRIP-LPDGVFDLTETGDGGVVMDTGTAVTRL 362

Query: 370 NKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHF-RGADVSLPAS 429
              AY+A RD F++  ++L  A   S+FDTCYDLSG  +V+VPTV  +F  G  ++LPA 
Sbjct: 363 PTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPAR 422

Query: 430 NYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGC 474
           N+L+PVD SG +CFAFA + +GLSIIGNIQQ+G +V +D A+  VGF P  C
Sbjct: 423 NFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008437888.10.0100.00PREDICTED: aspartyl protease family protein 2 [Cucumis melo] >KAA0048886.1 prote... [more]
XP_004133810.20.097.25aspartyl protease family protein 2 [Cucumis sativus] >KGN56421.1 hypothetical pr... [more]
XP_038877929.16.34e-31292.80aspartyl protease family protein 2-like [Benincasa hispida][more]
XP_023527618.11.04e-29789.29aspartyl protease family protein 2-like [Cucurbita pepo subsp. pepo][more]
XP_022924595.12.19e-29789.08aspartyl protease family protein 2-like [Cucurbita moschata] >KAG6582675.1 Aspar... [more]
Match NameE-valueIdentityDescription
Q9LNJ36.4e-18369.55Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q9LS401.2e-10949.24Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LHE33.6e-10946.19Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LEW37.5e-7540.05Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1[more]
Q8S9J63.2e-7338.65Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Match NameE-valueIdentityDescription
A0A5A7U3R30.0100.00Protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3AV660.0100.00aspartyl protease family protein 2 OS=Cucumis melo OX=3656 GN=LOC103483183 PE=3 ... [more]
A0A0A0L8K00.097.25Aspartic proteinase nepenthesin-1 OS=Cucumis sativus OX=3659 GN=Csa_3G119540 PE=... [more]
A0A6J1EFG41.06e-29789.08aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1IY261.45e-29788.84aspartyl protease family protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111479... [more]
Match NameE-valueIdentityDescription
AT1G01300.14.5e-18469.55Eukaryotic aspartyl protease family protein [more]
AT3G61820.14.6e-16864.48Eukaryotic aspartyl protease family protein [more]
AT1G25510.12.5e-11355.14Eukaryotic aspartyl protease family protein [more]
AT3G18490.18.7e-11149.24Eukaryotic aspartyl protease family protein [more]
AT3G20015.12.5e-11046.19Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 445..460
score: 26.83
coord: 138..158
score: 40.7
coord: 351..362
score: 39.97
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 43..473
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 132..297
e-value: 1.1E-54
score: 185.3
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 318..469
e-value: 4.3E-36
score: 124.1
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 110..293
e-value: 4.9E-54
score: 185.3
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 294..474
e-value: 4.4E-54
score: 185.0
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 125..473
NoneNo IPR availablePANTHERPTHR13683:SF761ASPARTYL PROTEASE FAMILY PROTEIN 2-LIKEcoord: 43..473
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 147..158
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 132..469
score: 46.836941
IPR033873CND41-likeCDDcd05472cnd41_likecoord: 131..473
e-value: 1.78236E-135
score: 390.479

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C006020.jh1.t1MELO3C006020.jh1.t1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity