MELO3C004787 (gene) Melon (DHL92) v4

Overview
NameMELO3C004787
Typegene
OrganismCucumis melo cv. DHL92 (Melon (DHL92) v4)
DescriptionCytoplasmic tRNA 2-thiolation protein 1
Locationchr12: 10175726 .. 10180150 (-)
RNA-Seq ExpressionMELO3C004787
SyntenyMELO3C004787
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAACCTTTCCCCGAAGGGTGTCCAACAGCTGCTTTTGGAAGTTGGCAATAGATCCTCGACCACCCTACTTCAGTTGAAGAACTTCTATCTCTTCTCGACGTTAGCCCTTCTGTTTTCCTTCTCTACGTTTTTCAGCAGCTTGTTTTTGTTTAGGGCTTTTCCGACGGACTGTGTTTTACTGAATTAGCTTTTCAGCCTGGTAGAATGCTGTCTTTGAATTTTCTTTTGCTCTACGTTATACTCTGATATTTTGATATATCTGAAGCTTTAGATTTCAAGTAGCACAACTTAAATTAGAACAATATTTGGTTTACATTTATTCAAGTTTACTGGAATTATTCTTTTGGGTAGGGTTACATATGTCTGAAAACTGTTAAGGAGGCAATTGATTGGGATTAAGTGAATGTTTGAAAACTGGTAAGGAGTGGATTGCTTCCCGGAATGTTGATAATGTGTCTTCGTGTAATGGGGGTTCGTGCTTTTTAGATCAGTCAGGCAGTACAATATCACTTAACATTTGTTACTGTGACTTTTAAATCTGTCTTTCACCTTCATTCCTTAAACTCCACATTGAATGTATAGTGAGGTATTTCCCCACCTCATTGCTTTTCTTATCCTGCTAATGTAGTTAGTTATTCTCCCTCTTCTCTTCCCTCATTCTGTTGAATTAAAATATAATGGAATACTAGCAGCCAAATGGGCCTTCGTGGTCTTTGGTATTTGTTTATTTGTTTGTTTGTCTTTATCTATATGGGATCTTAATTTTTGTGGTTATGACAACTGACAAGCGCTTTGCATGTTTCTTTGACTTCACAATAATTTATAATCGGCTTGATTGCAGAAAATTGAGCGTTTGCTGGCAAAAGTTAAACAATCCCCTTCAAAATCAATGCTAATTGCGCTCACTCCATCACAGAATGCCTTGGTTTCTGATCGACTTCTTAGGCATTCAAATATTAATGTCAAAGTTGCAGTTGCAGCATGCGTTAGTGAAATAACTCGGATCATTGCACCTAATGCCCCTTACAGTGATGGAAATATGAAGGTAATCAGAAATTTGCTCGATTGAATGCCAATTTCCCACTTGTCGAATCTACTTCTAAAGAGTTGAATGTATGCTGCTGTTCAGGAGGTATTTCATCTTATAGTATCATCCTTTGAAAATCTCTCTGACAAGTCAAGTCGATCATATGTAAAATGCGCATCCATTCTTGAAACTGTTCCGAGGGTCAGATTATATGTGGCCATGCTGGATTTGGAATGTGATGCATTGATATGTTCCAACATTTCCTTAAGACAGTAAGGTATTATACTAGATTTTGTTTTGAAATAAAATTCCTTTTTAAATTTTTACTTATAAGACTATTTGTGGTAGCTATTAATTTTCCAACAATTCAAGTTCTTTTGTGAACAAATATAATTTGTTTCTCCATGAAATACTACTAGACTTCTGTTTTGAATCAGAACTTGACTTACTCCAGTATGACCATTATATTGGGTGTTTTGAATGAGATGTAGCAGTGGTATTTGAGTACATTTGAATGATTGTGTTAGACCCCCCAATATTACACACATATAAGAGAAGGGGCAGAAACGGGAATTAACATGGCTTATGCCAATTCGGGGAGCAAAGAGAGAGAAAGCATCTCCTGAGGGCCCACTGGGGAGTTGGTATTTATATAAAATGTCTTTGGGGATTGCTGTCATTAGGTTTTATGTTGTCTTTTTAGGTGAAGGCCGCCATAGCCTTCATTTGGGAGAAGTTTCCAGCCTTCTCTAAGGAGCTGGGTGTTAGTATTGTTTTTACTATTATTTTCTGGATTATAGCTCTGTTTTGTGGAACATTTTGAGTGTTATGATCAATATATTATAACAGAGTGCTGCCTCTGTTTTGTCTTGTTGGGACTTTTCTGCTTTTCAGGTTAAGAATCTTAACAAATTATCAAGAAAAACTCGGTTAAAAATATAATGCAGTGAATTAGTTGACTGTATGCATTGTAATAAGTTTGGCTTTGAACTATTGTCTGTTAAATAGGTTTTATTGGTTGAATGTGAATCCTTGTTACACTACAAGAAATTCTGGATCTCCTGACGTTGGTTTACACCGTCGGGAGATAAGGCATCTTCTGACGCAACAATTAATGAGTTGGGAGATGCAATATCCCCTGATGCCTTCATGCACGGCCTCGGAGATAGAAGAGAACTCCCGACGCCGTTAGTAATGCATCGGAGAACTGCTGACACTTTAATTAAAACGTTGACAGTTTTGGGATAACTCTCGACACTGTGTCGGGCTTTGTCGGAAGTTGGTCTTTAAAAACCCTCCTAGATTTGTATTTCACAGATCAGTGAAGAAGGGGAAGGGGAAATGAGAGACTCAGCTGAAAACCACCACCGTCGCTCGTGGCATCGCCCCGCCGCCGTTCTCGTCGTTTGCCCCGTCTCTTCTGGTGCCGCTGCTGTCCTCCTGTGTCTTTCTTGCTGCTGCTGCCCCTTTCTCTTCCGATATGTAACTTATTTAAGTTAGATTTAAATTTTGCCCTAAAATCCTAATTTGTTCAAAAACAATTGATTTCTAAAGGATTATGAGAGATGGTTTTTCAAAATCTATTGATTTCATAAGAGAGTAAAAAAGTGGTTATTAGCCATAATCTATTGATAGCCTTCTTCACTGTTTTTTGTTAATTCTCCCTTTGAGTTTTATTGGCCGTCTCTTATCCAAAGAATATTCTTCTCTATTAAGGCTTGTGGAAGCTTCCCTATCATTGTTTATCTCCTTCATTTTGTTCTGTTTGATTGGTGGTTATTGAAGGGACTCAATATCTTTTCTCTGAATGCTGATTTTTCAAGGTTTCAATGTATTTTCAAGACAAGCGTTGGCCTTAGCTTATTTGATTTGAAGTTTCCAGAGATAAAGCTTCTTTTTGTTGGTGTCTCTCTCCATTCTATTTGCAGAATTTTGTGACTTATGCTTAACCAGGATTGTATCTATTCTAGTTTAGGTATCTTGTATTGATCTATGTATTACTATAAGCATACTTGGCAAGTTCTCTTTTTGGTTCTTTGCGTATTAAGCATGGTTTCATTGTATTGATGGAAAATTTTCTCCTGAAGGCTCTTGATCGAGGTGCTGCATTGTTGAAAGTAGTCAAGCTTGCCACAAGGCACAATGCAATTGATATGGCTGAAACAGTTCTATTAGATATATTACGAGGTGATATTGCCAGGTATTTTAATAGTAAGACTTTTTTTCTGTCAATTACTTTTGTCCCTCATTTGGGCTGTATTAGATTGAAGTGATGCTTTGAATTTGTAGATTGAGTAGATGTACTGCAATAAGTACCGATGAAGATGGACCAATTCCAAGATGCAAACCTTTCAAGTACACATATGAAAAGAAGATAGTAGTATATTTTTATATTTTAGTCTCATAGCTCTAGAGCAAGTCTTGCATCATGCAGCACTCAATTTGTTTATTGGTTGATTCTAGTTGTAAACCTTAACGATAGATGTTCACGTATGATTACTTCAAGAAGCTGGACTACTTCTCCACCGAGTGTAAGTTTTTTTTTCTTTTAGTTGTCTTTTTTGTTTTATTTTATTTTATTTTCCACTCTTCTGCATCTTACTAATTGATAAGAAGTACAAATTCATTCTGTGCATTTTACATGGCTAGGCATAATTAGCTATAAAATTTGATTGATTGAATGTCAAGTTGTGATCGGTCAATAATTAACTATGAAATTTGTGTCCTAAGATTTCTTGTTTTTCATGTGGTTTCTTGTCATACCCTATTTAACTTTTTGGTTTGATTTTGTTTACGTGAAGAGTCTTTCTTTGTTTTGAGAATTTGTTGAAGAAGTTTAATATTTGTTATGTGGAGATTTGAAGATGTTGATTTTGTTTATGTGGAGATGTGTACGTATATATACTTGTTATGTGGAGATTTGTAGAGTTGATTTTGTTTATGTGGAGATTTGCAAAATTTTATAATTTGAACACTTTGGATCTTTTGGTTAAGATTTAGACGATAATTTTGTTCTAATTTTTTTTGGATTAAATTTCAGTGTTTTAGAATTGTCTCTAACATTTGCTTATTCTTTCTCTAATTAAGTAACTAATAAATATTTTTTTCCTTCTCTCACAGAATTCAAGAACTTTCCAAGTTTCAACAAATTAGAGACTTTGTTGAAGTTGTTTGAATGAATCTATCACTTTACATGGTTAGTTGTTGCTTTATTCTTTCTATATAAGTTGCTTTATTATATATATGATGTAATGAAGAAGTGAGTTCAAAATTTTAGGTTACAAAAATTTAAAAAATAAAATAAAAGTAATAATGGAATGTATTTTTTCTTAAAAAAATGATCGAACACCTGATGCACAAATATTAGGCGTCGGGAGTTTATAATTCCCGACACGTTG

mRNA sequence

GAACCTTTCCCCGAAGGGTGTCCAACAGCTGCTTTTGGAAGTTGGCAATAGATCCTCGACCACCCTACTTCAGTTGAAGAACTTCTATCTCTTCTCGACAAAATTGAGCGTTTGCTGGCAAAAGTTAAACAATCCCCTTCAAAATCAATGCTAATTGCGCTCACTCCATCACAGAATGCCTTGGTTTCTGATCGACTTCTTAGGCATTCAAATATTAATGTCAAAGTTGCAGTTGCAGCATGCGTTAGTGAAATAACTCGGATCATTGCACCTAATGCCCCTTACAGTGATGGAAATATGAAGAAGGCTCTTGATCGAGGTGCTGCATTGTTGAAAGTAGTCAAGCTTGCCACAAGGCACAATGCAATTGATATGGCTGAAACAGTTCTATTAGATATATTACGAGGTGATATTGCCAGATTGAGTAGATGTACTGCAATAAGTACCGATGAAGATGGACCAATTCCAAGATGCAAACCTTTCAAGTACACATATGAAAAGAAGATAGTAGTATATTTTTATATTTTAGTCTCATAGCTCTAGAGCAAGTCTTGCATCATGCAGCACTCAATTTGTTTATTGGTTGATTCTAGTTGTAAACCTTAACGATAGATGTTCACGTATGATTACTTCAAGAAGCTGGACTACTTCTCCACCGAGTAATTCAAGAACTTTCCAAGTTTCAACAAATTAGAGACTTTGTTGAAGTTGTTTGAATGAATCTATCACTTTACATGGTTAGTTGTTGCTTTATTCTTTCTATATAAGTTGCTTTATTATATATATGATGTAATGAAGAAGTGAGTTCAAAATTTTAGGTTACAAAAATTTAAAAAATAAAATAAAAGTAATAATGGAATGTATTTTTTCTTAAAAAAATGATCGAACACCTGATGCACAAATATTAGGCGTCGGGAGTTTATAATTCCCGACACGTTG

Coding sequence (CDS)

ATGCTAATTGCGCTCACTCCATCACAGAATGCCTTGGTTTCTGATCGACTTCTTAGGCATTCAAATATTAATGTCAAAGTTGCAGTTGCAGCATGCGTTAGTGAAATAACTCGGATCATTGCACCTAATGCCCCTTACAGTGATGGAAATATGAAGAAGGCTCTTGATCGAGGTGCTGCATTGTTGAAAGTAGTCAAGCTTGCCACAAGGCACAATGCAATTGATATGGCTGAAACAGTTCTATTAGATATATTACGAGGTGATATTGCCAGATTGAGTAGATGTACTGCAATAAGTACCGATGAAGATGGACCAATTCCAAGATGCAAACCTTTCAAGTACACATATGAAAAGAAGATAGTAGTATATTTTTATATTTTAGTCTCATAG

Protein sequence

MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIARLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFYILVS
Homology
[Close]
BLAST of MELO3C004787 vs. ExPASy Swiss-Prot
Match: O64862 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Arabidopsis thaliana OX=3702 GN=NCS6 PE=2 SV=2)

HSP 1 Score: 124.0 bits (310), Expect = 1.2e-27
Identity = 64/95 (67.37%), Postives = 76/95 (80.00%), Query Frame = 0 

Query: 33  VSEITRIIA--PNAPYSDGNMKKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI ++I    N  +     ++ALDRGAALLKV KL T HNA D+AETVLL+ILRGDIA
Sbjct: 136 MDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVEKLVTGHNADDIAETVLLNILRGDIA 195

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFY 126
           RLSRCT+I+T EDGPIPRCKPFKYTYEK+IV+Y Y
Sbjct: 196 RLSRCTSITTGEDGPIPRCKPFKYTYEKEIVMYAY 230

[Close]
BLAST of MELO3C004787 vs. ExPASy Swiss-Prot
Match: Q6Z6G6 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=NCS6 PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.0e-26
Identity = 59/74 (79.73%), Postives = 67/74 (90.54%), Query Frame = 0 

Query: 52  KKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIARLSRCTAISTDEDGPIPRCKP 111
           ++ALDRGAALLKV K+ T HNA D+AETVLL+ILRGDIARLSRCT I+T EDGPIPRCKP
Sbjct: 163 RQALDRGAALLKVDKIVTGHNADDIAETVLLNILRGDIARLSRCTFITTGEDGPIPRCKP 222

Query: 112 FKYTYEKKIVVYFY 126
           FKYTYEK+IV+Y Y
Sbjct: 223 FKYTYEKEIVMYAY 236

[Close]
BLAST of MELO3C004787 vs. ExPASy Swiss-Prot
Match: Q05AW7 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Xenopus laevis OX=8355 GN=ctu1 PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.0e-21
Identity = 49/74 (66.22%), Postives = 61/74 (82.43%), Query Frame = 0 

Query: 52  KKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIARLSRCTAISTDEDGPIPRCKP 111
           ++ALDRGA +L + K+ T HNA D+AETVL++ LRGDIARL RCTAI+T  +G IPRCKP
Sbjct: 148 RQALDRGAMMLGINKICTGHNADDIAETVLMNFLRGDIARLRRCTAITTGSEGAIPRCKP 207

Query: 112 FKYTYEKKIVVYFY 126
            KYTYEK+IV+Y Y
Sbjct: 208 LKYTYEKEIVLYAY 221

[Close]
BLAST of MELO3C004787 vs. ExPASy Swiss-Prot
Match: Q803X1 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Danio rerio OX=7955 GN=ctu1 PE=2 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 2.9e-21
Identity = 51/76 (67.11%), Postives = 62/76 (81.58%), Query Frame = 0 

Query: 52  KKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIARLSRCTAIST--DEDGPIPRC 111
           ++ALDRGA +L V K+ T HNA D+AETVL+++LRGDIARL RCTAIST  + DG IPRC
Sbjct: 148 RQALDRGAMMLNVDKICTGHNADDVAETVLMNVLRGDIARLRRCTAISTSSEGDGAIPRC 207

Query: 112 KPFKYTYEKKIVVYFY 126
           KP KY YEK+IV+Y Y
Sbjct: 208 KPLKYAYEKEIVLYAY 223

[Close]
BLAST of MELO3C004787 vs. ExPASy Swiss-Prot
Match: Q5FW05 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Xenopus tropicalis OX=8364 GN=ctu1 PE=2 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 6.5e-21
Identity = 48/74 (64.86%), Postives = 60/74 (81.08%), Query Frame = 0 

Query: 52  KKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIARLSRCTAISTDEDGPIPRCKP 111
           ++ALDRGA +L V K+ T HNA D+AETVL++ LRGDIARL RCT+I+T  +G IPRCKP
Sbjct: 148 RQALDRGAMMLGVNKICTGHNADDIAETVLMNFLRGDIARLRRCTSITTGSEGAIPRCKP 207

Query: 112 FKYTYEKKIVVYFY 126
            KY YEK+IV+Y Y
Sbjct: 208 LKYAYEKEIVLYAY 221

[Close]
BLAST of MELO3C004787 vs. NCBI nr
Match: TYK20310.1 (cytoplasmic tRNA 2-thiolation protein 1 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 186.4 bits (472), Expect = 1.5e-43
Identity = 121/247 (48.99%), Postives = 124/247 (50.20%), Query Frame = 0 

Query: 1   MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKK------- 60
           MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMK+       
Sbjct: 1   MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKEVFHLIVS 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  SFENLSDKSSRSYVKCASILETVPRVKAAIAFIWEKFPAFSKELGSAASVLSCWDFSAFQ 120

Query: 121 -------------------------------------------------------ALDRG 126
                                                                  ALDRG
Sbjct: 121 ISEEGEGEMRDSAENHHRRSWHRPAAVLVVCPVSSGAAAVLLCLSCCCCPFLFRYALDRG 180

[Close]
BLAST of MELO3C004787 vs. NCBI nr
Match: KAA0040949.1 (protein starmaker [Cucumis melo var. makuwa])

HSP 1 Score: 165.6 bits (418), Expect = 2.8e-37
Identity = 111/234 (47.44%), Postives = 112/234 (47.86%), Query Frame = 0 

Query: 1   MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKK------- 60
           MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMK+       
Sbjct: 1   MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKEVFHLIVS 60

Query: 61  ------------------------------------------------------------ 113
                                                                       
Sbjct: 61  SFENLSDKSSRSYVKCASILETVPRVKAAIAFIWEKFPAFSKELGSAASVLSCWDFSAFQ 120

[Close]
BLAST of MELO3C004787 vs. NCBI nr
Match: KAG7020429.1 (Cytoplasmic tRNA 2-thiolation protein 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 129.8 bits (325), Expect = 1.7e-26
Identity = 69/99 (69.70%), Postives = 80/99 (80.81%), Query Frame = 0 

Query: 33  VSEITRIIA--PNAPYSDGNMKKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI ++I    N  +     ++ALDRGAALLKV KLAT HNA DMAETVLL+ILRGDIA
Sbjct: 138 MDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHNADDMAETVLLNILRGDIA 197

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFYILVS 130
           RLSRCTAI T EDGPIPRCKPFKYTYEK+IV+Y +I+VS
Sbjct: 198 RLSRCTAIITGEDGPIPRCKPFKYTYEKEIVIYPFIVVS 236

[Close]
BLAST of MELO3C004787 vs. NCBI nr
Match: KAF8026266.1 (hypothetical protein BT93_F2926 [Corymbia citriodora subsp. variegata])

HSP 1 Score: 127.5 bits (319), Expect = 8.4e-26
Identity = 67/95 (70.53%), Postives = 77/95 (81.05%), Query Frame = 0 

Query: 33  VSEITRIIA--PNAPYSDGNMKKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI +II    N  +     ++ALDRGAALLKV KLAT HNA D+AETVLL+ILRGDIA
Sbjct: 139 MDEIVKIIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHNADDIAETVLLNILRGDIA 198

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFY 126
           RLSRCTAI+T EDGPIPRCKPFKYTYEK+IV+Y Y
Sbjct: 199 RLSRCTAITTGEDGPIPRCKPFKYTYEKEIVMYAY 233

[Close]
BLAST of MELO3C004787 vs. NCBI nr
Match: XP_030443958.1 (cytoplasmic tRNA 2-thiolation protein 1-like [Syzygium oleosum])

HSP 1 Score: 127.5 bits (319), Expect = 8.4e-26
Identity = 67/95 (70.53%), Postives = 77/95 (81.05%), Query Frame = 0 

Query: 33  VSEITRIIA--PNAPYSDGNMKKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI +II    N  +     ++ALDRGAALLKV KLAT HNA D+AETVLL+ILRGDIA
Sbjct: 25  MDEIVKIIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHNANDIAETVLLNILRGDIA 84

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFY 126
           RLSRCTAI+T EDGPIPRCKPFKYTYEK+IV+Y Y
Sbjct: 85  RLSRCTAITTGEDGPIPRCKPFKYTYEKEIVMYAY 119

[Close]
BLAST of MELO3C004787 vs. ExPASy TrEMBL
Match: A0A5D3DA09 (Cytoplasmic tRNA 2-thiolation protein 1 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold708G00390 PE=4 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 7.4e-44
Identity = 121/247 (48.99%), Postives = 124/247 (50.20%), Query Frame = 0 

Query: 1   MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKK------- 60
           MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMK+       
Sbjct: 1   MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKEVFHLIVS 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  SFENLSDKSSRSYVKCASILETVPRVKAAIAFIWEKFPAFSKELGSAASVLSCWDFSAFQ 120

Query: 121 -------------------------------------------------------ALDRG 126
                                                                  ALDRG
Sbjct: 121 ISEEGEGEMRDSAENHHRRSWHRPAAVLVVCPVSSGAAAVLLCLSCCCCPFLFRYALDRG 180

[Close]
BLAST of MELO3C004787 vs. ExPASy TrEMBL
Match: A0A5A7TI13 (Protein starmaker OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold125G001460 PE=4 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 1.3e-37
Identity = 111/234 (47.44%), Postives = 112/234 (47.86%), Query Frame = 0 

Query: 1   MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKK------- 60
           MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMK+       
Sbjct: 1   MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKEVFHLIVS 60

Query: 61  ------------------------------------------------------------ 113
                                                                       
Sbjct: 61  SFENLSDKSSRSYVKCASILETVPRVKAAIAFIWEKFPAFSKELGSAASVLSCWDFSAFQ 120

[Close]
BLAST of MELO3C004787 vs. ExPASy TrEMBL
Match: A0A059BW08 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Eucalyptus grandis OX=71139 GN=NCS6 PE=3 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 4.1e-26
Identity = 67/95 (70.53%), Postives = 77/95 (81.05%), Query Frame = 0 

Query: 33  VSEITRIIA--PNAPYSDGNMKKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI +II    N  +     ++ALDRGAALLKV KLAT HNA D+AETVLL+ILRGDIA
Sbjct: 139 MDEIVKIIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHNADDIAETVLLNILRGDIA 198

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFY 126
           RLSRCTAI+T EDGPIPRCKPFKYTYEK+IV+Y Y
Sbjct: 199 RLSRCTAITTGEDGPIPRCKPFKYTYEKEIVMYAY 233

[Close]
BLAST of MELO3C004787 vs. ExPASy TrEMBL
Match: A0A6J1KNU4 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111496254 PE=3 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 6.9e-26
Identity = 67/95 (70.53%), Postives = 76/95 (80.00%), Query Frame = 0 

Query: 33  VSEITRIIA--PNAPYSDGNMKKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI ++I    N  +     ++ALDRGAALLKV KLAT HNA DMAETVLL+ILRGDIA
Sbjct: 138 MDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHNADDMAETVLLNILRGDIA 197

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFY 126
           RLSRCTAI T EDGPIPRCKPFKYTYEK+IV+Y Y
Sbjct: 198 RLSRCTAIITGEDGPIPRCKPFKYTYEKEIVMYAY 232

[Close]
BLAST of MELO3C004787 vs. ExPASy TrEMBL
Match: A0A0A0LKF0 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Cucumis sativus OX=3659 GN=NCS6 PE=3 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 6.9e-26
Identity = 67/95 (70.53%), Postives = 76/95 (80.00%), Query Frame = 0 

Query: 33  VSEITRIIA--PNAPYSDGNMKKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI ++I    N  +     ++ALDRGAALLKV KLAT HNA DMAETVLL+ILRGDIA
Sbjct: 138 MDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHNADDMAETVLLNILRGDIA 197

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFY 126
           RLSRCTAI T EDGPIPRCKPFKYTYEK+IV+Y Y
Sbjct: 198 RLSRCTAIITGEDGPIPRCKPFKYTYEKEIVMYAY 232

[Close]
BLAST of MELO3C004787 vs. TAIR 10
Match: AT2G44270.1 (repressor of lrx1 )

HSP 1 Score: 124.0 bits (310), Expect = 8.7e-29
Identity = 64/95 (67.37%), Postives = 76/95 (80.00%), Query Frame = 0 

Query: 33  VSEITRIIA--PNAPYSDGNMKKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI ++I    N  +     ++ALDRGAALLKV KL T HNA D+AETVLL+ILRGDIA
Sbjct: 136 MDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVEKLVTGHNADDIAETVLLNILRGDIA 195

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFY 126
           RLSRCT+I+T EDGPIPRCKPFKYTYEK+IV+Y Y
Sbjct: 196 RLSRCTSITTGEDGPIPRCKPFKYTYEKEIVMYAY 230

[Close]
BLAST of MELO3C004787 vs. TAIR 10
Match: AT1G76170.1 (2-thiocytidine tRNA biosynthesis protein, TtcA )

HSP 1 Score: 122.5 bits (306), Expect = 2.5e-28
Identity = 63/95 (66.32%), Postives = 76/95 (80.00%), Query Frame = 0 

Query: 33  VSEITRIIA--PNAPYSDGNMKKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI ++I    N  +     ++ALDRGAALLKV KL T HNA D+AETV+L+ILRGDIA
Sbjct: 114 MDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLVTGHNADDIAETVVLNILRGDIA 173

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFY 126
           RLSRCT+I+T EDGPIPRCKPFKYTYEK+IV+Y Y
Sbjct: 174 RLSRCTSITTGEDGPIPRCKPFKYTYEKEIVMYAY 208

[Close]
BLAST of MELO3C004787 vs. TAIR 10
Match: AT2G44270.2 (repressor of lrx1 )

HSP 1 Score: 108.6 bits (270), Expect = 3.8e-24
Identity = 56/86 (65.12%), Postives = 67/86 (77.91%), Query Frame = 0 

Query: 33  VSEITRIIA--PNAPYSDGNMKKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI ++I    N  +     ++ALDRGAALLKV KL T HNA D+AETVLL+ILRGDIA
Sbjct: 146 MDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVEKLVTGHNADDIAETVLLNILRGDIA 205

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTY 117
           RLSRCT+I+T EDGPIPRCKPF +TY
Sbjct: 206 RLSRCTSITTGEDGPIPRCKPFNFTY 231

[Close]
BLAST of MELO3C004787 vs. TAIR 10
Match: AT4G31880.1 (LOCATED IN: cytosol, chloroplast; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 14 growth stages; BEST Arabidopsis thaliana protein match is: Tudor/PWWP/MBT superfamily protein (TAIR:AT1G15940.1); Has 137162 Blast hits to 70781 proteins in 2973 species: Archae - 289; Bacteria - 24182; Metazoa - 56725; Fungi - 20130; Plants - 6559; Viruses - 758; Other Eukaryotes - 28519 (source: NCBI BLink). )

HSP 1 Score: 67.0 bits (162), Expect = 1.3e-11
Identity = 31/50 (62.00%), Postives = 39/50 (78.00%), Query Frame = 0 

Query: 4   ALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKK 54
           ALTP    LV  +L +HS+++VKVAVAAC+SEITRI AP+APY D  MK+
Sbjct: 54  ALTPLMKGLVGGKLFKHSDVDVKVAVAACISEITRITAPDAPYDDDQMKE 103

[Close]
BLAST of MELO3C004787 vs. TAIR 10
Match: AT4G31880.2 (LOCATED IN: cytosol; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 14 growth stages; BEST Arabidopsis thaliana protein match is: Tudor/PWWP/MBT superfamily protein (TAIR:AT1G15940.1). )

HSP 1 Score: 67.0 bits (162), Expect = 1.3e-11
Identity = 31/50 (62.00%), Postives = 39/50 (78.00%), Query Frame = 0 

Query: 4   ALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKK 54
           ALTP    LV  +L +HS+++VKVAVAAC+SEITRI AP+APY D  MK+
Sbjct: 54  ALTPLMKGLVGGKLFKHSDVDVKVAVAACISEITRITAPDAPYDDDQMKE 103

The following BLAST results are available for this feature:
BLAST of MELO3C004787 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O648621.2e-2767.37Cytoplasmic tRNA 2-thiolation protein 1 OS=Arabidopsis thaliana OX=3702 GN=NCS6 ... [more]
Q6Z6G61.0e-2679.73Cytoplasmic tRNA 2-thiolation protein 1 OS=Oryza sativa subsp. japonica OX=39947... [more]
Q05AW71.0e-2166.22Cytoplasmic tRNA 2-thiolation protein 1 OS=Xenopus laevis OX=8355 GN=ctu1 PE=2 S... [more]
Q803X12.9e-2167.11Cytoplasmic tRNA 2-thiolation protein 1 OS=Danio rerio OX=7955 GN=ctu1 PE=2 SV=1[more]
Q5FW056.5e-2164.86Cytoplasmic tRNA 2-thiolation protein 1 OS=Xenopus tropicalis OX=8364 GN=ctu1 PE... [more]
BLAST of MELO3C004787 vs. NCBI nr
Match NameE-valueIdentityDescription
TYK20310.11.5e-4348.99cytoplasmic tRNA 2-thiolation protein 1 isoform X2 [Cucumis melo var. makuwa][more]
KAA0040949.12.8e-3747.44protein starmaker [Cucumis melo var. makuwa][more]
KAG7020429.11.7e-2669.70Cytoplasmic tRNA 2-thiolation protein 1 [Cucurbita argyrosperma subsp. argyrospe... [more]
KAF8026266.18.4e-2670.53hypothetical protein BT93_F2926 [Corymbia citriodora subsp. variegata][more]
XP_030443958.18.4e-2670.53cytoplasmic tRNA 2-thiolation protein 1-like [Syzygium oleosum][more]
BLAST of MELO3C004787 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5D3DA097.4e-4448.99Cytoplasmic tRNA 2-thiolation protein 1 isoform X2 OS=Cucumis melo var. makuwa O... [more]
A0A5A7TI131.3e-3747.44Protein starmaker OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold125G00... [more]
A0A059BW084.1e-2670.53Cytoplasmic tRNA 2-thiolation protein 1 OS=Eucalyptus grandis OX=71139 GN=NCS6 P... [more]
A0A6J1KNU46.9e-2670.53Cytoplasmic tRNA 2-thiolation protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111496... [more]
A0A0A0LKF06.9e-2670.53Cytoplasmic tRNA 2-thiolation protein 1 OS=Cucumis sativus OX=3659 GN=NCS6 PE=3 ... [more]
BLAST of MELO3C004787 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G44270.18.7e-2967.37repressor of lrx1 [more]
AT1G76170.12.5e-2866.322-thiocytidine tRNA biosynthesis protein, TtcA [more]
AT2G44270.23.8e-2465.12repressor of lrx1 [more]
AT4G31880.11.3e-1162.00LOCATED IN: cytosol, chloroplast; EXPRESSED IN: 24 plant structures; EXPRESSED D... [more]
AT4G31880.21.3e-1162.00LOCATED IN: cytosol; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 14 gro... [more]
InterPro
Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 15..127
e-value: 2.0E-14
score: 55.3
IPR000541Cytoplasmic tRNA 2-thiolation protein 1PANTHERPTHR11807:SF12CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 1coord: 52..125
NoneNo IPR availablePANTHERPTHR11807ATPASES OF THE PP SUPERFAMILY-RELATEDcoord: 52..125
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 53..124

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C004787.1MELO3C004787.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034227 tRNA thio-modification
biological_process GO:0002098 tRNA wobble uridine modification
molecular_function GO:0000049 tRNA binding