MELO3C004637 (gene) Melon (DHL92) v4

Overview


Name	MELO3C004637
Type	gene
Organism	Cucumis melo cv. DHL92 (Melon (DHL92) v4)
Description	Pre-mRNA cleavage factor Im 25 kDa subunit
Location	chr05: 28868833 .. 28871398 (-)
RNA-Seq Expression	MELO3C004637
Synteny	MELO3C004637

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCTATTCTCAAATTCCATCTCGTCCCTTCCCTTTTGCTTTCCCAAGTGGTCTTCTTGTCCGTCTCTTATTCTAGTCTTTAATTAGACTTTAGAATTAGAAATTCCGCAGAGTGAGTGACGCCATTCCACTTCTTCGCTTCGTGTCCCTTCTCTCCATCCAACACGGCACCTTACTTCTTCTTCATACTTCCTACTTCCACCTTACCTATATTCCCTTTTGATTCTCCTTCTGACCTTCTTTTCTCAGTCCCCGCCATGGGAGACGACGGGCGTCTGCATGCTATTGACGACGACGACGACGATGCTTCTTCCGCCCACCAACTCTCTTACATCGATATCTATCCTCTCAGCAACTACTACTTTGGATCCAAAGAGCCTCTTCTTTTCAAGGACGAGACCTTGTCTGATCGTATCCTCAGGATGAAATCCAAGTATCCATTCTCACTTTATTCCGTTGACTATACTTTGCTCTTTAATACCTTCCTTCAATTTATCTATTTTGTTTTCTTTTTCTGCAGTTATGCTGCTTACGGATTGAGGACTTGCGTCGAAGCAGTTATGCTGGTTCGTTCCTTCCTTACAATTATTTTATTTTATATACTTCCATTTCCGCAATCTTCTGTCTCCTAAGTCCTACTGATTGATTTCTTTAAGGATATAGAGTTTTTATTCTGCCTTTGCTGTTACCGTCCTCCAATTGCTTGACGCGTACTTAATCGGTGGAGTTCTGACACTGTCGGCTCCTGTCAGTGTAGTTATAACTTCTACTGCAGTCTAAACAGTGGCCTATTCTCCCGCTATTCTTAGATTTATAGATCATGATGCCTGATTACTAAGAGTAGCTGTTATGAAATCAACAAATAAAATTAAGAAGAAAAGTTCGAATTGTAGGAAACTGATACAAAGATTAACATGTTCACTCATAGTGTGTGAGTTTATTATTAGAGAATAATTCAGGATACAGATGGTTTCTAAGTTTAGAGTTTACTTAGCGAACTTTTCTAAATTCCGTAGTAAAGAGTATAACTAACATAAATAGGCTAATACAAATACAAATACAATTTAGGTTTCAACAATAATTGAGTATAGCTTTCAGTTAAATAATGATTCGCAAGTTCTGTCTCCTGGCCGTATTGGCTTTTGTGGGATTGGTTCTTTATTTTTGTGGAATGAACATGAATTTTTGCATCCGTACTTGATCTCCATACTTCGTTTCCGTTTTGGGAGAAATCATGGTAGGTCGGCCTTTTCTATCCATAATACCTTTTAGGAAATAAGTTTTAGAATTGTGAATCTGAATTCAGGACGCTTTTTTCTGCTATACAATTTCTAGATGGACATTTCATCTTGCGATCAGAAACAGTGCTTAGTATTCTGCATTATAATTGAAAGTGCCTGTTGTTCTCAAATTAGGTTGAACTATTCAAGCATCCTCATTTATTGCTGTTTCAAATACGTAATTCAATTTTCAAGCTTCCTGGTGGTCGCCTAAGGCCCAACGAATCAGGTAAATTTGTTCTTTGGATCTCTTCTGAAGCTGAGTTTGTCTAAGTCTAATAGTTATGCTGATATTTTGTCATGGCTGATGATCTCAATTTACAGATATTGATGGCTTGACACGAAAGTTGTCGAAGAAGCTTTGTGCCAATGGAGCCTCTGATGCATCTGATTGGGAGGTATTGTAATGCCCAAATCTATTCCTTTAATTTCTCTACCATATTACTGCTGCGATTGTGAATGATGGAATTTGAAATTTTCTGCTTTTAACGTTTAGGTTAACGAGTGCCTCGGTATGTGGTGGAGGCCTGACTTTGAGACCTTGCTCTTTCCGTATTTGTCCACTAATGTAAAAGGGGCGAAGGTAGTTATTTGAATGTGAACTACTTCCTTTAAACTACGATATGCATTGTTTACCTTTAACTCACTCGCTCCCTACCTGATGCAGGAGTGCGCTAAACTTTTCTTGGTCAAGTTGCCGGAGAGTAAAAAATTTGTTGTGCCAAAGAACCTCAAGTTGATCGCAGTTCCTTTATGCCAAATTCACGAAAACCATAAGGTATCTTAATCAAGTCATTTCATGGAAGCCCAATATGTAACATTTGAAAATCTTACTTTTTGTTGAAGGATTGAAAGAAAAGGAGACGAAGTTCAATTTTTGAAACAAGCCTATATATTGTTGATTAAAAGTGAGGTGCATAATCTAACTACATGTAAATCCCGTCATCTTGCAGACATATGGACCGGTTATATCAGGCATTCCGCAGCTTCTTTCTAAGTTCTCCTTCAACATCATCGGAACATAAGACTCACATCTTATTAATTATAACAGATGAAGAACTATGGTCGGAGAAAAAAATATTTATTAGTGCATTATAGAGCTGTCAGCTAACTTTATATGAATGTTGACAGACCCTTGTATAAAACATATTATAATCACACAAACATATATGTTGATGATTTTGTAAGTATAGAGATATATATAGTAGTAATATAGGTCCACATTGTGACTTTACAACTTGTGTGTTAAATGAGTGCTAAAAACTACATATGATTGTGTAAATTGCT

mRNA sequence

TTCTATTCTCAAATTCCATCTCGTCCCTTCCCTTTTGCTTTCCCAAGTGGTCTTCTTGTCCGTCTCTTATTCTAGTCTTTAATTAGACTTTAGAATTAGAAATTCCGCAGAGTGAGTGACGCCATTCCACTTCTTCGCTTCGTGTCCCTTCTCTCCATCCAACACGGCACCTTACTTCTTCTTCATACTTCCTACTTCCACCTTACCTATATTCCCTTTTGATTCTCCTTCTGACCTTCTTTTCTCAGTCCCCGCCATGGGAGACGACGGGCGTCTGCATGCTATTGACGACGACGACGACGATGCTTCTTCCGCCCACCAACTCTCTTACATCGATATCTATCCTCTCAGCAACTACTACTTTGGATCCAAAGAGCCTCTTCTTTTCAAGGACGAGACCTTGTCTGATCGTATCCTCAGGATGAAATCCAATTATGCTGCTTACGGATTGAGGACTTGCGTCGAAGCAGTTATGCTGGTTGAACTATTCAAGCATCCTCATTTATTGCTGTTTCAAATACGTAATTCAATTTTCAAGCTTCCTGGTGGTCGCCTAAGGCCCAACGAATCAGATATTGATGGCTTGACACGAAAGTTGTCGAAGAAGCTTTGTGCCAATGGAGCCTCTGATGCATCTGATTGGGAGGTTAACGAGTGCCTCGGTATGTGGTGGAGGCCTGACTTTGAGACCTTGCTCTTTCCGTATTTGTCCACTAATGTAAAAGGGGCGAAGGAGTGCGCTAAACTTTTCTTGGTCAAGTTGCCGGAGAGTAAAAAATTTGTTGTGCCAAAGAACCTCAAGTTGATCGCAGTTCCTTTATGCCAAATTCACGAAAACCATAAGACATATGGACCGGTTATATCAGGCATTCCGCAGCTTCTTTCTAAGTTCTCCTTCAACATCATCGGAACATAAGACTCACATCTTATTAATTATAACAGATGAAGAACTATGGTCGGAGAAAAAAATATTTATTAGTGCATTATAGAGCTGTCAGCTAACTTTATATGAATGTTGACAGACCCTTGTATAAAACATATTATAATCACACAAACATATATGTTGATGATTTTGTAAGTATAGAGATATATATAGTAGTAATATAGGTCCACATTGTGACTTTACAACTTGTGTGTTAAATGAGTGCTAAAAACTACATATGATTGTGTAAATTGCT

Coding sequence (CDS)

ATGGGAGACGACGGGCGTCTGCATGCTATTGACGACGACGACGACGATGCTTCTTCCGCCCACCAACTCTCTTACATCGATATCTATCCTCTCAGCAACTACTACTTTGGATCCAAAGAGCCTCTTCTTTTCAAGGACGAGACCTTGTCTGATCGTATCCTCAGGATGAAATCCAATTATGCTGCTTACGGATTGAGGACTTGCGTCGAAGCAGTTATGCTGGTTGAACTATTCAAGCATCCTCATTTATTGCTGTTTCAAATACGTAATTCAATTTTCAAGCTTCCTGGTGGTCGCCTAAGGCCCAACGAATCAGATATTGATGGCTTGACACGAAAGTTGTCGAAGAAGCTTTGTGCCAATGGAGCCTCTGATGCATCTGATTGGGAGGTTAACGAGTGCCTCGGTATGTGGTGGAGGCCTGACTTTGAGACCTTGCTCTTTCCGTATTTGTCCACTAATGTAAAAGGGGCGAAGGAGTGCGCTAAACTTTTCTTGGTCAAGTTGCCGGAGAGTAAAAAATTTGTTGTGCCAAAGAACCTCAAGTTGATCGCAGTTCCTTTATGCCAAATTCACGAAAACCATAAGACATATGGACCGGTTATATCAGGCATTCCGCAGCTTCTTTCTAAGTTCTCCTTCAACATCATCGGAACATAA

Protein sequence

MGDDGRLHAIDDDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRILRMKSNYAAYGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKLCANGASDASDWEVNECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKNLKLIAVPLCQIHENHKTYGPVISGIPQLLSKFSFNIIGT

Homology

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BLAST of MELO3C004637 vs. NCBI nr
Match: XP_008459551.1 (PREDICTED: pre-mRNA cleavage factor Im 25 kDa subunit 1 [Cucumis melo])

HSP 1 Score: 451.4 bits (1160), Expect = 4.3e-123
Identity = 219/219 (100.00%), Postives = 219/219 (100.00%), Query Frame = 0


Query: 1   MGDDGRLHAIDDDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRILRMKSNY 60
           MGDDGRLHAIDDDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRILRMKSNY
Sbjct: 1   MGDDGRLHAIDDDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRILRMKSNY 60

Query: 61  AAYGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKLCA 120
           AAYGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKLCA
Sbjct: 61  AAYGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKLCA 120

Query: 121 NGASDASDWEVNECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKN 180
           NGASDASDWEVNECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKN
Sbjct: 121 NGASDASDWEVNECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKN 180

Query: 181 LKLIAVPLCQIHENHKTYGPVISGIPQLLSKFSFNIIGT 220
           LKLIAVPLCQIHENHKTYGPVISGIPQLLSKFSFNIIGT
Sbjct: 181 LKLIAVPLCQIHENHKTYGPVISGIPQLLSKFSFNIIGT 219

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BLAST of MELO3C004637 vs. NCBI nr
Match: XP_004141062.1 (pre-mRNA cleavage factor Im 25 kDa subunit 1 [Cucumis sativus] >KGN60522.1 hypothetical protein Csa_019305 [Cucumis sativus])

HSP 1 Score: 427.2 bits (1097), Expect = 8.7e-116
Identity = 207/219 (94.52%), Postives = 214/219 (97.72%), Query Frame = 0


Query: 1   MGDDGRLHAIDDDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRILRMKSNY 60
           MGDDGRLHAI  DDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDR+LRMKSNY
Sbjct: 1   MGDDGRLHAI--DDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRVLRMKSNY 60

Query: 61  AAYGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKLCA 120
           AA+GLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGR+RPNESDIDGLTRKL+KKL A
Sbjct: 61  AAHGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRIRPNESDIDGLTRKLTKKLSA 120

Query: 121 NGASDASDWEVNECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKN 180
           NGASDAS WEV+ECLGMWWRPDFETLLFPYLSTNVKGAKEC KLFLVKLPES+KFVVPKN
Sbjct: 121 NGASDASHWEVSECLGMWWRPDFETLLFPYLSTNVKGAKECTKLFLVKLPESRKFVVPKN 180

Query: 181 LKLIAVPLCQIHENHKTYGPVISGIPQLLSKFSFNIIGT 220
           LKLIAVPLCQIHENHKTYGP+ISGIPQLLSKFSFNIIGT
Sbjct: 181 LKLIAVPLCQIHENHKTYGPIISGIPQLLSKFSFNIIGT 217

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BLAST of MELO3C004637 vs. NCBI nr
Match: KAA0061480.1 (T-complex protein 1 subunit beta [Cucumis melo var. makuwa])

HSP 1 Score: 404.8 bits (1039), Expect = 4.6e-109
Identity = 195/204 (95.59%), Postives = 200/204 (98.04%), Query Frame = 0


Query: 1   MGDDGRLHAIDDDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRILRMKSNY 60
           MGDDGRLHAIDDDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRILRMKSNY
Sbjct: 1   MGDDGRLHAIDDDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRILRMKSNY 60

Query: 61  AAYGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKLCA 120
           AAYGLRTCVEAVML+ELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKL A
Sbjct: 61  AAYGLRTCVEAVMLIELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKLSA 120

Query: 121 NGASDASDWEVNECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKN 180
           NGASDASDWEVNECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKN
Sbjct: 121 NGASDASDWEVNECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKN 180

Query: 181 LKLIAVPLCQIHENHKTYGPVISG 205
           LKLIAVPLCQIHENHK++   ++G
Sbjct: 181 LKLIAVPLCQIHENHKSFDHSLTG 204

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BLAST of MELO3C004637 vs. NCBI nr
Match: TYK10794.1 (T-complex protein 1 subunit beta [Cucumis melo var. makuwa])

HSP 1 Score: 402.9 bits (1034), Expect = 1.8e-108
Identity = 194/204 (95.10%), Postives = 200/204 (98.04%), Query Frame = 0


Query: 1   MGDDGRLHAIDDDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRILRMKSNY 60
           MGDDGRLHAIDDDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDR+LRMKSNY
Sbjct: 1   MGDDGRLHAIDDDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRVLRMKSNY 60

Query: 61  AAYGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKLCA 120
           AAYGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKL A
Sbjct: 61  AAYGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKLSA 120

Query: 121 NGASDASDWEVNECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKN 180
           NGASDASDWEV+ECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKN
Sbjct: 121 NGASDASDWEVSECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKN 180

Query: 181 LKLIAVPLCQIHENHKTYGPVISG 205
           LKLIAVPLCQIHENHK++   ++G
Sbjct: 181 LKLIAVPLCQIHENHKSFDHSLTG 204

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BLAST of MELO3C004637 vs. NCBI nr
Match: XP_038889512.1 (pre-mRNA cleavage factor Im 25 kDa subunit 1 [Benincasa hispida])

HSP 1 Score: 394.4 bits (1012), Expect = 6.3e-106
Identity = 193/219 (88.13%), Postives = 204/219 (93.15%), Query Frame = 0


Query: 1   MGDDGRLHAIDDDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRILRMKSNY 60
           MGDDGRLHA+  DDD+AS+A Q   I IYPLSNYYF SKEPLLFKDET +DR+LRMKSNY
Sbjct: 1   MGDDGRLHAM--DDDEASAADQRPSIHIYPLSNYYFESKEPLLFKDETFADRLLRMKSNY 60

Query: 61  AAYGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKLCA 120
           AAYGLRT VEAV+LVELFKHPHLLLFQIRNSIFKLPGGRLRPNESD+DGLTRKLS+KL A
Sbjct: 61  AAYGLRTSVEAVLLVELFKHPHLLLFQIRNSIFKLPGGRLRPNESDVDGLTRKLSRKLSA 120

Query: 121 NGASDASDWEVNECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKN 180
           NGASDASDWEV ECLGMWWRPDFETLLFPYLS+ VKGAKEC KLFLVKLPES+KFVVPKN
Sbjct: 121 NGASDASDWEVGECLGMWWRPDFETLLFPYLSSTVKGAKECTKLFLVKLPESRKFVVPKN 180

Query: 181 LKLIAVPLCQIHENHKTYGPVISGIPQLLSKFSFNIIGT 220
           LKLIAVPLCQIHENHKTYGPVISGIPQLLSKFSF+II T
Sbjct: 181 LKLIAVPLCQIHENHKTYGPVISGIPQLLSKFSFDIIET 217

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BLAST of MELO3C004637 vs. ExPASy Swiss-Prot
Match: Q94AF0 (Pre-mRNA cleavage factor Im 25 kDa subunit 1 OS=Arabidopsis thaliana OX=3702 GN=CFIS1 PE=1 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 6.1e-80
Identity = 139/220 (63.18%), Postives = 178/220 (80.91%), Query Frame = 0


Query: 1   MGDDGRLHAIDDDDDDASSAHQLSY---IDIYPLSNYYFGSKEPLLFKDETLSDRILRMK 60
           MG++ R   +++  D+ +  + + +   +D+YPLS+YYFGSKE L  KDE +SDR++R+K
Sbjct: 1   MGEEARALDMEEISDNTTRRNDVVHDLMVDLYPLSSYYFGSKEALRVKDEIISDRVIRLK 60

Query: 61  SNYAAYGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKK 120
           SNYAA+GLRTCVEAV+LVELFKHPH+LL Q RNSIFKLPGGRLRP ESDI+GL RKL+ K
Sbjct: 61  SNYAAHGLRTCVEAVLLVELFKHPHVLLLQYRNSIFKLPGGRLRPGESDIEGLKRKLASK 120

Query: 121 LCANGASDASDWEVNECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVV 180
           L  N     S +EV EC+GMWWRP+FETL++P+L  N+K  KEC KLFLV+LP  ++FVV
Sbjct: 121 LSVNENVGVSGYEVGECIGMWWRPNFETLMYPFLPPNIKHPKECTKLFLVRLPVHQQFVV 180

Query: 181 PKNLKLIAVPLCQIHENHKTYGPVISGIPQLLSKFSFNII 218
           PKN KL+AVPLCQ+HEN KTYGP++S IP+LLSKFSFN++
Sbjct: 181 PKNFKLLAVPLCQLHENEKTYGPIMSQIPKLLSKFSFNMM 220

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BLAST of MELO3C004637 vs. ExPASy Swiss-Prot
Match: Q8GXS3 (Pre-mRNA cleavage factor Im 25 kDa subunit 2 OS=Arabidopsis thaliana OX=3702 GN=CFIS2 PE=1 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 1.2e-59
Identity = 104/194 (53.61%), Postives = 142/194 (73.20%), Query Frame = 0


Query: 26  IDIYPLSNYYFGSKEPLLFKDETLSDRILRMKSNYAAYGLRTCVEAVMLVELFKHPHLLL 85
           ++ YPLSNY FG+KEP L KD +++DR+ RMK NY   G+RT VE ++LV+   HPH+LL
Sbjct: 7   VNTYPLSNYSFGTKEPKLEKDTSVADRLARMKINYMKEGMRTSVEGILLVQEHNHPHILL 66

Query: 86  FQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKLCANGASDASDWEVNECLGMWWRPDFET 145
            QI N+  KLPGGRL+P E++ DGL RKL+ KL  N A+   DW V EC+  WWRP+FET
Sbjct: 67  LQIGNTFCKLPGGRLKPGENEADGLKRKLTSKLGGNSAALVPDWTVGECVATWWRPNFET 126

Query: 146 LLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKNLKLIAVPLCQIHENHKTYGPVISGI 205
           +++PY   ++   KEC +L++V L E + F VPKNLKL+AVPL ++++N + YGPVIS I
Sbjct: 127 MMYPYCPPHITKPKECKRLYIVHLSEKEYFAVPKNLKLLAVPLFELYDNVQRYGPVISTI 186

Query: 206 PQLLSKFSFNIIGT 220
           PQ LS+F FN+I +
Sbjct: 187 PQQLSRFHFNMISS 200

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BLAST of MELO3C004637 vs. ExPASy Swiss-Prot
Match: Q7T3C6 (Cleavage and polyadenylation specificity factor subunit 5 OS=Danio rerio OX=7955 GN=nudt21 PE=2 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 5.2e-47
Identity = 87/191 (45.55%), Postives = 128/191 (67.02%), Query Frame = 0


Query: 26  IDIYPLSNYYFGSKEPLLFKDETLSDRILRMKSNYAAYGLRTCVEAVMLVELFKHPHLLL 85
           I++YPL+NY FG+KEPL  KD +++ R  RM+  +   G+R  VE V++V   + PH+LL
Sbjct: 38  INLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFEKIGMRRTVEGVLIVHEHRLPHVLL 97

Query: 86  FQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKLCANGASDA--SDWEVNECLGMWWRPDF 145
            Q+  + FKLPGG L P E +++GL R +++ L   G  D    DW +++C+G WWRP+F
Sbjct: 98  LQLGTTFFKLPGGELNPGEDEVEGLKRLMTEIL---GRQDGVKQDWVIDDCIGNWWRPNF 157

Query: 146 ETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKNLKLIAVPLCQIHENHKTYGPVIS 205
           E   +PY+  ++   KE  KLFLV+L E   F VPKN KL+A PL ++++N   YGP+IS
Sbjct: 158 EPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGPIIS 217

Query: 206 GIPQLLSKFSF 215
            +PQLLS+F+F
Sbjct: 218 SLPQLLSRFNF 225

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BLAST of MELO3C004637 vs. ExPASy Swiss-Prot
Match: Q3ZCA2 (Cleavage and polyadenylation specificity factor subunit 5 OS=Bos taurus OX=9913 GN=NUDT21 PE=2 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 6.8e-47
Identity = 87/191 (45.55%), Postives = 128/191 (67.02%), Query Frame = 0


Query: 26  IDIYPLSNYYFGSKEPLLFKDETLSDRILRMKSNYAAYGLRTCVEAVMLVELFKHPHLLL 85
           I++YPL+NY FG+KEPL  KD +++ R  RM+  +   G+R  VE V++V   + PH+LL
Sbjct: 37  INLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRTVEGVLIVHEHRLPHVLL 96

Query: 86  FQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKLCANGASDA--SDWEVNECLGMWWRPDF 145
            Q+  + FKLPGG L P E +++GL R +++ L   G  D    DW +++C+G WWRP+F
Sbjct: 97  LQLGTTFFKLPGGELNPGEDEVEGLKRLMTEIL---GRQDGVLQDWVIDDCIGNWWRPNF 156

Query: 146 ETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKNLKLIAVPLCQIHENHKTYGPVIS 205
           E   +PY+  ++   KE  KLFLV+L E   F VPKN KL+A PL ++++N   YGP+IS
Sbjct: 157 EPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGPIIS 216

Query: 206 GIPQLLSKFSF 215
            +PQLLS+F+F
Sbjct: 217 SLPQLLSRFNF 224

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BLAST of MELO3C004637 vs. ExPASy Swiss-Prot
Match: O43809 (Cleavage and polyadenylation specificity factor subunit 5 OS=Homo sapiens OX=9606 GN=NUDT21 PE=1 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 6.8e-47
Identity = 87/191 (45.55%), Postives = 128/191 (67.02%), Query Frame = 0


Query: 26  IDIYPLSNYYFGSKEPLLFKDETLSDRILRMKSNYAAYGLRTCVEAVMLVELFKHPHLLL 85
           I++YPL+NY FG+KEPL  KD +++ R  RM+  +   G+R  VE V++V   + PH+LL
Sbjct: 37  INLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRTVEGVLIVHEHRLPHVLL 96

Query: 86  FQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKLCANGASDA--SDWEVNECLGMWWRPDF 145
            Q+  + FKLPGG L P E +++GL R +++ L   G  D    DW +++C+G WWRP+F
Sbjct: 97  LQLGTTFFKLPGGELNPGEDEVEGLKRLMTEIL---GRQDGVLQDWVIDDCIGNWWRPNF 156

Query: 146 ETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKNLKLIAVPLCQIHENHKTYGPVIS 205
           E   +PY+  ++   KE  KLFLV+L E   F VPKN KL+A PL ++++N   YGP+IS
Sbjct: 157 EPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGPIIS 216

Query: 206 GIPQLLSKFSF 215
            +PQLLS+F+F
Sbjct: 217 SLPQLLSRFNF 224

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BLAST of MELO3C004637 vs. ExPASy TrEMBL
Match: A0A1S3C9Z9 (Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucumis melo OX=3656 GN=LOC103498651 PE=3 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 2.1e-123
Identity = 219/219 (100.00%), Postives = 219/219 (100.00%), Query Frame = 0


Query: 1   MGDDGRLHAIDDDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRILRMKSNY 60
           MGDDGRLHAIDDDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRILRMKSNY
Sbjct: 1   MGDDGRLHAIDDDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRILRMKSNY 60

Query: 61  AAYGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKLCA 120
           AAYGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKLCA
Sbjct: 61  AAYGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKLCA 120

Query: 121 NGASDASDWEVNECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKN 180
           NGASDASDWEVNECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKN
Sbjct: 121 NGASDASDWEVNECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKN 180

Query: 181 LKLIAVPLCQIHENHKTYGPVISGIPQLLSKFSFNIIGT 220
           LKLIAVPLCQIHENHKTYGPVISGIPQLLSKFSFNIIGT
Sbjct: 181 LKLIAVPLCQIHENHKTYGPVISGIPQLLSKFSFNIIGT 219

[Close]

BLAST of MELO3C004637 vs. ExPASy TrEMBL
Match: A0A0A0LIM0 (Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucumis sativus OX=3659 GN=Csa_2G000310 PE=3 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 4.2e-116
Identity = 207/219 (94.52%), Postives = 214/219 (97.72%), Query Frame = 0


Query: 1   MGDDGRLHAIDDDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRILRMKSNY 60
           MGDDGRLHAI  DDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDR+LRMKSNY
Sbjct: 1   MGDDGRLHAI--DDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRVLRMKSNY 60

Query: 61  AAYGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKLCA 120
           AA+GLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGR+RPNESDIDGLTRKL+KKL A
Sbjct: 61  AAHGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRIRPNESDIDGLTRKLTKKLSA 120

Query: 121 NGASDASDWEVNECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKN 180
           NGASDAS WEV+ECLGMWWRPDFETLLFPYLSTNVKGAKEC KLFLVKLPES+KFVVPKN
Sbjct: 121 NGASDASHWEVSECLGMWWRPDFETLLFPYLSTNVKGAKECTKLFLVKLPESRKFVVPKN 180

Query: 181 LKLIAVPLCQIHENHKTYGPVISGIPQLLSKFSFNIIGT 220
           LKLIAVPLCQIHENHKTYGP+ISGIPQLLSKFSFNIIGT
Sbjct: 181 LKLIAVPLCQIHENHKTYGPIISGIPQLLSKFSFNIIGT 217

[Close]

BLAST of MELO3C004637 vs. ExPASy TrEMBL
Match: A0A5A7V2I9 (CCT-beta OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G00400 PE=3 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 2.2e-109
Identity = 195/204 (95.59%), Postives = 200/204 (98.04%), Query Frame = 0


Query: 1   MGDDGRLHAIDDDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRILRMKSNY 60
           MGDDGRLHAIDDDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRILRMKSNY
Sbjct: 1   MGDDGRLHAIDDDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRILRMKSNY 60

Query: 61  AAYGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKLCA 120
           AAYGLRTCVEAVML+ELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKL A
Sbjct: 61  AAYGLRTCVEAVMLIELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKLSA 120

Query: 121 NGASDASDWEVNECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKN 180
           NGASDASDWEVNECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKN
Sbjct: 121 NGASDASDWEVNECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKN 180

Query: 181 LKLIAVPLCQIHENHKTYGPVISG 205
           LKLIAVPLCQIHENHK++   ++G
Sbjct: 181 LKLIAVPLCQIHENHKSFDHSLTG 204

[Close]

BLAST of MELO3C004637 vs. ExPASy TrEMBL
Match: A0A5D3CI10 (CCT-beta OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G001090 PE=3 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 8.5e-109
Identity = 194/204 (95.10%), Postives = 200/204 (98.04%), Query Frame = 0


Query: 1   MGDDGRLHAIDDDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRILRMKSNY 60
           MGDDGRLHAIDDDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDR+LRMKSNY
Sbjct: 1   MGDDGRLHAIDDDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRVLRMKSNY 60

Query: 61  AAYGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKLCA 120
           AAYGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKL A
Sbjct: 61  AAYGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKLSA 120

Query: 121 NGASDASDWEVNECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKN 180
           NGASDASDWEV+ECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKN
Sbjct: 121 NGASDASDWEVSECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKN 180

Query: 181 LKLIAVPLCQIHENHKTYGPVISG 205
           LKLIAVPLCQIHENHK++   ++G
Sbjct: 181 LKLIAVPLCQIHENHKSFDHSLTG 204

[Close]

BLAST of MELO3C004637 vs. ExPASy TrEMBL
Match: A0A6J1FHN4 (Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucurbita moschata OX=3662 GN=LOC111444047 PE=3 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 1.0e-101
Identity = 179/219 (81.74%), Postives = 202/219 (92.24%), Query Frame = 0


Query: 1   MGDDGRLHAIDDDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRILRMKSNY 60
           MGDDGR+HA+  +DD++++A Q+  IDIYPLSNYYFGSKEPL+FKD+TL+DR++RMKSNY
Sbjct: 1   MGDDGRMHAM--EDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNY 60

Query: 61  AAYGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKLCA 120
           AAYGLRT VEAV+LVELFKHPH+LL QIRNSIF LPGGRLRPNESDIDGL RKLS+KL A
Sbjct: 61  AAYGLRTSVEAVLLVELFKHPHVLLLQIRNSIFNLPGGRLRPNESDIDGLKRKLSRKLSA 120

Query: 121 NGASDASDWEVNECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKN 180
           NGA DASDWEV +CLG WWRPDFETLL+P+LS NVKGAKEC KLFLVKLPE++KF+VPKN
Sbjct: 121 NGAPDASDWEVGDCLGTWWRPDFETLLYPHLSNNVKGAKECTKLFLVKLPENRKFIVPKN 180

Query: 181 LKLIAVPLCQIHENHKTYGPVISGIPQLLSKFSFNIIGT 220
           LKLIAVPLCQ+HENHK YGPVISGIPQLLSKFSFNII T
Sbjct: 181 LKLIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIET 217

[Close]

BLAST of MELO3C004637 vs. TAIR 10
Match: AT4G29820.1 (homolog of CFIM-25 )

HSP 1 Score: 298.5 bits (763), Expect = 4.4e-81
Identity = 139/220 (63.18%), Postives = 178/220 (80.91%), Query Frame = 0


Query: 1   MGDDGRLHAIDDDDDDASSAHQLSY---IDIYPLSNYYFGSKEPLLFKDETLSDRILRMK 60
           MG++ R   +++  D+ +  + + +   +D+YPLS+YYFGSKE L  KDE +SDR++R+K
Sbjct: 1   MGEEARALDMEEISDNTTRRNDVVHDLMVDLYPLSSYYFGSKEALRVKDEIISDRVIRLK 60

Query: 61  SNYAAYGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKK 120
           SNYAA+GLRTCVEAV+LVELFKHPH+LL Q RNSIFKLPGGRLRP ESDI+GL RKL+ K
Sbjct: 61  SNYAAHGLRTCVEAVLLVELFKHPHVLLLQYRNSIFKLPGGRLRPGESDIEGLKRKLASK 120

Query: 121 LCANGASDASDWEVNECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVV 180
           L  N     S +EV EC+GMWWRP+FETL++P+L  N+K  KEC KLFLV+LP  ++FVV
Sbjct: 121 LSVNENVGVSGYEVGECIGMWWRPNFETLMYPFLPPNIKHPKECTKLFLVRLPVHQQFVV 180

Query: 181 PKNLKLIAVPLCQIHENHKTYGPVISGIPQLLSKFSFNII 218
           PKN KL+AVPLCQ+HEN KTYGP++S IP+LLSKFSFN++
Sbjct: 181 PKNFKLLAVPLCQLHENEKTYGPIMSQIPKLLSKFSFNMM 220

[Close]

BLAST of MELO3C004637 vs. TAIR 10
Match: AT4G25550.1 (Cleavage/polyadenylation specificity factor, 25kDa subunit )

HSP 1 Score: 231.1 bits (588), Expect = 8.5e-61
Identity = 104/194 (53.61%), Postives = 142/194 (73.20%), Query Frame = 0


Query: 26  IDIYPLSNYYFGSKEPLLFKDETLSDRILRMKSNYAAYGLRTCVEAVMLVELFKHPHLLL 85
           ++ YPLSNY FG+KEP L KD +++DR+ RMK NY   G+RT VE ++LV+   HPH+LL
Sbjct: 7   VNTYPLSNYSFGTKEPKLEKDTSVADRLARMKINYMKEGMRTSVEGILLVQEHNHPHILL 66

Query: 86  FQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKLCANGASDASDWEVNECLGMWWRPDFET 145
            QI N+  KLPGGRL+P E++ DGL RKL+ KL  N A+   DW V EC+  WWRP+FET
Sbjct: 67  LQIGNTFCKLPGGRLKPGENEADGLKRKLTSKLGGNSAALVPDWTVGECVATWWRPNFET 126

Query: 146 LLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKNLKLIAVPLCQIHENHKTYGPVISGI 205
           +++PY   ++   KEC +L++V L E + F VPKNLKL+AVPL ++++N + YGPVIS I
Sbjct: 127 MMYPYCPPHITKPKECKRLYIVHLSEKEYFAVPKNLKLLAVPLFELYDNVQRYGPVISTI 186

Query: 206 PQLLSKFSFNIIGT 220
           PQ LS+F FN+I +
Sbjct: 187 PQQLSRFHFNMISS 200

The following BLAST results are available for this feature:

BLAST of MELO3C004637 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008459551.1	4.3e-123	100.00	PREDICTED: pre-mRNA cleavage factor Im 25 kDa subunit 1 [Cucumis melo]	[more]
XP_004141062.1	8.7e-116	94.52	pre-mRNA cleavage factor Im 25 kDa subunit 1 [Cucumis sativus] >KGN60522.1 hypot...	[more]
KAA0061480.1	4.6e-109	95.59	T-complex protein 1 subunit beta [Cucumis melo var. makuwa]	[more]
TYK10794.1	1.8e-108	95.10	T-complex protein 1 subunit beta [Cucumis melo var. makuwa]	[more]
XP_038889512.1	6.3e-106	88.13	pre-mRNA cleavage factor Im 25 kDa subunit 1 [Benincasa hispida]	[more]

BLAST of MELO3C004637 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94AF0	6.1e-80	63.18	Pre-mRNA cleavage factor Im 25 kDa subunit 1 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q8GXS3	1.2e-59	53.61	Pre-mRNA cleavage factor Im 25 kDa subunit 2 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q7T3C6	5.2e-47	45.55	Cleavage and polyadenylation specificity factor subunit 5 OS=Danio rerio OX=7955...	[more]
Q3ZCA2	6.8e-47	45.55	Cleavage and polyadenylation specificity factor subunit 5 OS=Bos taurus OX=9913 ...	[more]
O43809	6.8e-47	45.55	Cleavage and polyadenylation specificity factor subunit 5 OS=Homo sapiens OX=960...	[more]

BLAST of MELO3C004637 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C9Z9	2.1e-123	100.00	Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucumis melo OX=3656 GN=LOC1034986...	[more]
A0A0A0LIM0	4.2e-116	94.52	Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucumis sativus OX=3659 GN=Csa_2G0...	[more]
A0A5A7V2I9	2.2e-109	95.59	CCT-beta OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G00400 PE=3 S...	[more]
A0A5D3CI10	8.5e-109	95.10	CCT-beta OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G001090 PE=3...	[more]
A0A6J1FHN4	1.0e-101	81.74	Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]

BLAST of MELO3C004637 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G29820.1	4.4e-81	63.18	homolog of CFIM-25	[more]
AT4G25550.1	8.5e-61	53.61	Cleavage/polyadenylation specificity factor, 25kDa subunit	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016706	Cleavage/polyadenylation specificity factor subunit 5	PFAM	PF13869	NUDIX_2	coord: 26..212 e-value: 1.8E-69 score: 233.1
IPR016706	Cleavage/polyadenylation specificity factor subunit 5	PIRSF	PIRSF017888	CPSF-25	coord: 3..219 e-value: 2.0E-78 score: 260.6
IPR016706	Cleavage/polyadenylation specificity factor subunit 5	PANTHER	PTHR13047	PRE-MRNA CLEAVAGE FACTOR IM, 25KD SUBUNIT	coord: 10..217
None	No IPR available	GENE3D	3.90.79.10	Nucleoside Triphosphate Pyrophosphohydrolase	coord: 24..216 e-value: 5.7E-74 score: 249.6
None	No IPR available	PANTHER	PTHR13047:SF1	PRE-MRNA CLEAVAGE FACTOR IM 25 KDA SUBUNIT 1	coord: 10..217

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C004637.1	MELO3C004637.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006378	mRNA polyadenylation
cellular_component	GO:0005849	mRNA cleavage factor complex
molecular_function	GO:0003729	mRNA binding