MELO3C004192 (gene) Melon (DHL92) v4

Overview


Name	MELO3C004192
Type	gene
Organism	Cucumis melo cv. DHL92 (Melon (DHL92) v4)
Description	RNA pseudouridine synthase 1
Location	chr05: 24871344 .. 24873968 (+)
RNA-Seq Expression	MELO3C004192
Synteny	MELO3C004192

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCCTCTTCATCTCACTTCATCCCAATTTCTCAATTTTCCCCAAAACCTTCGCCAAATTCCCATTCACAAAACCCTTCTGTTTCCACAGATTCAAATCCGTCAGCAATAGCATGACGGACTCATCGGAACCGGTGACCGGAAACTGCCCATCAACCGGAGAGAATTACCCGGTCCCCTTATCGCCTCCACTGCCGGCCATATCCAAGAACTTAGAGCTCGCCAGAGCCATGGCTGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCGGCAAACGATGTTATATACGAAGACGAGTGGCTCATTGCCGTCAATAAACCTCAAGGAATCTACTGTGAAAATGTTTTGGCTGCTGTTCCTCGTCTTCTTTGTGATTCGGCTAACGCTGGTTTGTGAATCTTACTGTGAAAGTGTTTTTTTTTTTCAGACGAATTCAACTTTTTACTACATTTTGGATTTTCTAATCGAATGGTAATGGTGGGTTTGCAACTTTTAACTTGCTTTCGTTTGACGATTTGTGGAAAGGGTATGTATAATCATGGACTTCCCGTGGATAACGAAAGATGTTAGAACTGAGTTTTCGTTAAAATTGACGGGTTTTGAAAGATTTTGTTCCATATAGGTCATTTGCATTGATTTTCTAGATCAAACTTTTGTATATTGATAATGTAACTTGGTTTTTGTTGAAAGTTAGCTGAAAGTAATGGGCGAATGCGATGAAAGTTTAACAGAAGTTTAAAATTGTAATGCTTATCTATGTGTACGGTCCAATTTGTTGAAAATTTGAAGTTTGGAGTAATAATTGATTCATATTGCCAAGCTTTTGGGTAGGATTTGATCTTTCATCAAATTTGTTTAGAACGAGTGATTTTCTATTGATTTAAGCTGATTAAAAATGCAATTCTCAAGTTCTATTTCCTCATCTGTAACTATCTTCTTGTAATTATTATGTAGGTGTTATTTTGCATAGTTGGAGTCTCTTCATATAGGGAGAGGCGCCTTTTCTCTGGGCTGTGCTTTTATTTTTATGCCGGTGTATTTTTTCTTTTTTTTCCAATAAAGGCCGGTGTTTCTAAAAGAAAATAATACGTTTACAGAAGAAGCAAGGGCGAGTAACTGATTTCTGATTCTTTTCCTACAATCTACATAGCCTAGCAATGTTGCTGATTGTTAGATAGTTGATATAAATGTTTTAGATGTATCCTGGTTGTTGAAGAGAGAAGGGTGCTTGAATGTTTCTGAAGTGTTTTAACGATTGTCCAAAACAATGCCATGTAATTACATCTTTTGCTAATTCAATCATTATCAATTTGTTGGGAGTGTTCTTAGCTCGAATTCTTCATATGCAGGGATCAAAACCAATTTGCCAGAGCTTCATCTTGCTAACCGCCTTGATCGTGACACAAGTGGGGTTATGGTTATAACGAAGTCACATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACCGACCACACGGTTTCAAAATCATATATCGCCTTCTGTGTTGGCACATCTCCAAAATGGAAAAAAATCCACGTCAAATCTGGTCATGGAAGGTCAAAGTTTGGGGTCTGGCGTGTCTATGCTGCAGCTGATGTGGGTCGTTCCTTACCTGGCGGTTCCATAGTCAGAGATATGGAAACATATTTTGAAGTACTGTCTGTAAATGGAAAAAACACCATGGAGGAATTACAAAAATTCAGGAAAGAGGAAGAAGAAACTATTGTAGTCCATACAAAATCTTTGGTGGACATTGATAGTCAGAAGGACGAGGTCTTGATAAGAGCAAGACCTCGCAGTGGAAGAACTCATCAAATCCGACTGCACTGCCAATATCTTGGAATTCCTATAAGAGGGGATGTGAAATATGAGGGTGTTACAGAGTGGAATGGGAAAACTTACGATAGCCATGAGCTTCATGCTGAAAGCTTGTACTTTGTGCATCCTATTACAGGTATTCCTCTCAAACTACAAGCTCCTTTACCATCATGGGCCAGCCAAGCGTTGCAGCCCCAACAACAAGAAGTGAATTCTCCACAGTCCTTCAAAACTATGCCTTAAGAAAATCATTGGCGCAATACAGTTAAGTGAGAACCAAATATATCCCTTATTATACAAGGAGATTCGGTGTTGAAGAATGTCGCTGAAGCAAAATGGAGCGATCTTCAATCAAATTCATAAAGTGCGACTGCAGACAGTTTTCGTACTGTCAAGTTGATGTCTGCAATTTTTCATCTATTGAATGAGCTCTTGTTTGCACGACAGTGTCTACAAAAATATCAGCAGTCGCAAACCAAGAAACCATCCGAGTTCTTGAAAATTTGAGCTCGAAATGGATTCTGGAAATGACCGAAGAATCATGTGTGTCAAAGGCTGATAAATTTGTGCAAAGCCCTTTGGAAATGGTTCTTCTGCAGTGGTTTTATCGATAAAATTGTTGTACGGTGCTAAAACAGCCAAATTCTGTGGTTGGCATGTTGATTTATATTGAGGCTCAATTGCTATCCGACTCCCCTTCCATCCATTTGTGCAACTTTGTCATCAAAATATGAACAATTTGTTATGGTGTTTATGCGGCTATGAATTATAAATAGAAGAATGAGAAACATGTTGTGGTTTATGTA

mRNA sequence

ATGGCCCTCTTCATCTCACTTCATCCCAATTTCTCAATTTTCCCCAAAACCTTCGCCAAATTCCCATTCACAAAACCCTTCTGTTTCCACAGATTCAAATCCGTCAGCAATAGCATGACGGACTCATCGGAACCGGTGACCGGAAACTGCCCATCAACCGGAGAGAATTACCCGGTCCCCTTATCGCCTCCACTGCCGGCCATATCCAAGAACTTAGAGCTCGCCAGAGCCATGGCTGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCGGCAAACGATGTTATATACGAAGACGAGTGGCTCATTGCCGTCAATAAACCTCAAGGAATCTACTGTGAAAATGTTTTGGCTGCTGTTCCTCGTCTTCTTTGTGATTCGGCTAACGCTGACGAATTCAACTTTTTACTACATTTTGGATTTTCTAATCGAATGGTGTTATTTTGCATAGTTGGAGTCTCTTCATATAGGGAGAGGCGCCTTTTCTCTGGGCTGCCGGTGTTTCTAAAAGAAAATAATACGTTTACAGAAGAAGCAAGGGCGAGGATCAAAACCAATTTGCCAGAGCTTCATCTTGCTAACCGCCTTGATCGTGACACAAGTGGGGTTATGGTTATAACGAAGTCACATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACCGACCACACGGTTTCAAAATCATATATCGCCTTCTGTGTTGGCACATCTCCAAAATGGAAAAAAATCCACGTCAAATCTGGTCATGGAAGGTCAAAGTTTGGGGTCTGGCGTGTCTATGCTGCAGCTGATGTGGGTCGTTCCTTACCTGGCGGTTCCATAGTCAGAGATATGGAAACATATTTTGAAGTACTGTCTGTAAATGGAAAAAACACCATGGAGGAATTACAAAAATTCAGGAAAGAGGAAGAAGAAACTATTGTAGTCCATACAAAATCTTTGGTGGACATTGATAGTCAGAAGGACGAGGTCTTGATAAGAGCAAGACCTCGCAGTGGAAGAACTCATCAAATCCGACTGCACTGCCAATATCTTGGAATTCCTATAAGAGGGGATGTGAAATATGAGGGTGTTACAGAGTGGAATGGGAAAACTTACGATAGCCATGAGCTTCATGCTGAAAGCTTGTACTTTGTGCATCCTATTACAGGTATTCCTCTCAAACTACAAGCTCCTTTACCATCATGGGCCAGCCAAGCGTTGCAGCCCCAACAACAAGAAGTGAATTCTCCACAGTCCTTCAAAACTATGCCTTAAGAAAATCATTGGCGCAATACAGTTAAGTGAGAACCAAATATATCCCTTATTATACAAGGAGATTCGGTGTTGAAGAATGTCGCTGAAGCAAAATGGAGCGATCTTCAATCAAATTCATAAAGTGCGACTGCAGACAGTTTTCGTACTGTCAAGTTGATGTCTGCAATTTTTCATCTATTGAATGAGCTCTTGTTTGCACGACAGTGTCTACAAAAATATCAGCAGTCGCAAACCAAGAAACCATCCGAGTTCTTGAAAATTTGAGCTCGAAATGGATTCTGGAAATGACCGAAGAATCATGTGTGTCAAAGGCTGATAAATTTGTGCAAAGCCCTTTGGAAATGGTTCTTCTGCAGTGGTTTTATCGATAAAATTGTTGTACGGTGCTAAAACAGCCAAATTCTGTGGTTGGCATGTTGATTTATATTGAGGCTCAATTGCTATCCGACTCCCCTTCCATCCATTTGTGCAACTTTGTCATCAAAATATGAACAATTTGTTATGGTGTTTATGCGGCTATGAATTATAAATAGAAGAATGAGAAACATGTTGTGGTTTATGTA

Coding sequence (CDS)

ATGGCCCTCTTCATCTCACTTCATCCCAATTTCTCAATTTTCCCCAAAACCTTCGCCAAATTCCCATTCACAAAACCCTTCTGTTTCCACAGATTCAAATCCGTCAGCAATAGCATGACGGACTCATCGGAACCGGTGACCGGAAACTGCCCATCAACCGGAGAGAATTACCCGGTCCCCTTATCGCCTCCACTGCCGGCCATATCCAAGAACTTAGAGCTCGCCAGAGCCATGGCTGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCGGCAAACGATGTTATATACGAAGACGAGTGGCTCATTGCCGTCAATAAACCTCAAGGAATCTACTGTGAAAATGTTTTGGCTGCTGTTCCTCGTCTTCTTTGTGATTCGGCTAACGCTGACGAATTCAACTTTTTACTACATTTTGGATTTTCTAATCGAATGGTGTTATTTTGCATAGTTGGAGTCTCTTCATATAGGGAGAGGCGCCTTTTCTCTGGGCTGCCGGTGTTTCTAAAAGAAAATAATACGTTTACAGAAGAAGCAAGGGCGAGGATCAAAACCAATTTGCCAGAGCTTCATCTTGCTAACCGCCTTGATCGTGACACAAGTGGGGTTATGGTTATAACGAAGTCACATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACCGACCACACGGTTTCAAAATCATATATCGCCTTCTGTGTTGGCACATCTCCAAAATGGAAAAAAATCCACGTCAAATCTGGTCATGGAAGGTCAAAGTTTGGGGTCTGGCGTGTCTATGCTGCAGCTGATGTGGGTCGTTCCTTACCTGGCGGTTCCATAGTCAGAGATATGGAAACATATTTTGAAGTACTGTCTGTAAATGGAAAAAACACCATGGAGGAATTACAAAAATTCAGGAAAGAGGAAGAAGAAACTATTGTAGTCCATACAAAATCTTTGGTGGACATTGATAGTCAGAAGGACGAGGTCTTGATAAGAGCAAGACCTCGCAGTGGAAGAACTCATCAAATCCGACTGCACTGCCAATATCTTGGAATTCCTATAAGAGGGGATGTGAAATATGAGGGTGTTACAGAGTGGAATGGGAAAACTTACGATAGCCATGAGCTTCATGCTGAAAGCTTGTACTTTGTGCATCCTATTACAGGTATTCCTCTCAAACTACAAGCTCCTTTACCATCATGGGCCAGCCAAGCGTTGCAGCCCCAACAACAAGAAGTGAATTCTCCACAGTCCTTCAAAACTATGCCTTAA

Protein sequence

MALFISLHPNFSIFPKTFAKFPFTKPFCFHRFKSVSNSMTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENVLAAVPRLLCDSANADEFNFLLHFGFSNRMVLFCIVGVSSYRERRLFSGLPVFLKENNTFTEEARARIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQALQPQQQEVNSPQSFKTMP

Homology

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BLAST of MELO3C004192 vs. NCBI nr
Match: XP_004144814.3 (RNA pseudouridine synthase 1 [Cucumis sativus] >KGN60992.1 hypothetical protein Csa_021313 [Cucumis sativus])

HSP 1 Score: 674.1 bits (1738), Expect = 7.8e-190
Identity = 346/417 (82.97%), Postives = 354/417 (84.89%), Query Frame = 0


Query: 1   MALFISLHPNFSIFPKTFAKFPFTKPFCFHRFKSVSNSMTDSSEPVTGNCPSTGENYPVP 60
           MALFISLHPNFSI PKTF +FPFTK FCFHRFKSVSNSMTDSSEPVTGNCPST ENYPVP
Sbjct: 1   MALFISLHPNFSISPKTFTEFPFTKSFCFHRFKSVSNSMTDSSEPVTGNCPSTAENYPVP 60

Query: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120
           LSPPLPAISKNLELARAM ASSKSSL+ALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP
Sbjct: 61  LSPPLPAISKNLELARAMVASSKSSLYALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120

Query: 121 RLLCDSANADEFNFLLHFGFSNRMVLFCIVGVSSYRERRLFSGLPVFLKENNTFTEEARA 180
           RLL DSANA                                                   
Sbjct: 121 RLLGDSANAG-------------------------------------------------- 180

Query: 181 RIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWK 240
            IKT+LPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWK
Sbjct: 181 -IKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWK 240

Query: 241 KIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRK 300
           KI+VKSGHGRSKFGVWRVYAAADVGRSLPGGS+VRDMETYFEVLSVNGKNTMEELQKFR+
Sbjct: 241 KINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNTMEELQKFRR 300

Query: 301 EEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEW 360
            EEETIVVHTKSLVDIDS KDE+LIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEW
Sbjct: 301 GEEETIVVHTKSLVDIDSHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEW 360

Query: 361 NGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQALQPQQQEVNSPQSFKTMP 418
           N K YDSHELHAESLYFVHP+TGIPLKLQAPLPSWASQALQPQQ EVNSPQSFKTMP
Sbjct: 361 NEKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQQHEVNSPQSFKTMP 366

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BLAST of MELO3C004192 vs. NCBI nr
Match: XP_008453651.1 (PREDICTED: RNA pseudouridine synthase 1 [Cucumis melo] >KAA0048062.1 RNA pseudouridine synthase 1 [Cucumis melo var. makuwa] >TYJ96474.1 RNA pseudouridine synthase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 634.4 bits (1635), Expect = 6.8e-178
Identity = 327/379 (86.28%), Postives = 327/379 (86.28%), Query Frame = 0


Query: 39  MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 98
           MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED
Sbjct: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60

Query: 99  EWLIAVNKPQGIYCENVLAAVPRLLCDSANADEFNFLLHFGFSNRMVLFCIVGVSSYRER 158
           EWLIAVNKPQGIYCENVLAAVPRLLCDSANA                             
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAG---------------------------- 120

Query: 159 RLFSGLPVFLKENNTFTEEARARIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKA 218
                                  IKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKA
Sbjct: 121 -----------------------IKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKA 180

Query: 219 FTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDME 278
           FTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDME
Sbjct: 181 FTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDME 240

Query: 279 TYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIR 338
           TYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIR
Sbjct: 241 TYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIR 300

Query: 339 LHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQ 398
           LHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQ
Sbjct: 301 LHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQ 328

Query: 399 ALQPQQQEVNSPQSFKTMP 418
           ALQPQQQEVNSPQSFKTMP
Sbjct: 361 ALQPQQQEVNSPQSFKTMP 328

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BLAST of MELO3C004192 vs. NCBI nr
Match: XP_038889468.1 (RNA pseudouridine synthase 1 [Benincasa hispida])

HSP 1 Score: 609.8 bits (1571), Expect = 1.8e-170
Identity = 319/410 (77.80%), Postives = 333/410 (81.22%), Query Frame = 0


Query: 1   MALFISLHPNFSIFPKTFAKFPFTKPFCFHRFKSVSNSMTDSSEPVTGNCPSTGENYPVP 60
           MAL ISL P FS   KTF +FP  KPFC  RFKS+S SM DSSEPVTGNCPST ENYPVP
Sbjct: 1   MALLISLQPTFSTPHKTFTEFP--KPFCSGRFKSISKSMADSSEPVTGNCPSTRENYPVP 60

Query: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120
           LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCE+VLAAVP
Sbjct: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVLAAVP 120

Query: 121 RLLCDSANADEFNFLLHFGFSNRMVLFCIVGVSSYRERRLFSGLPVFLKENNTFTEEARA 180
           RLL DS                                                   A A
Sbjct: 121 RLLSDS---------------------------------------------------AMA 180

Query: 181 RIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWK 240
            IKT+LPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVG++PKWK
Sbjct: 181 GIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGSAPKWK 240

Query: 241 KIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRK 300
           KI++KSGHGRSKFGVWRVYAAADVGRSLPGGS+VRDMETYFEVLS+NG+N MEE QKF K
Sbjct: 241 KINIKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSINGQNAMEEFQKFGK 300

Query: 301 EEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEW 360
            +EET+VV TKSLVDID QKDE+LIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEW
Sbjct: 301 -DEETVVVQTKSLVDIDGQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEW 356

Query: 361 NGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQALQPQQQEVNSP 411
           NGK YD HELHAESLYFVHPITGIPLKLQAPLPSWASQA QPQQQEVNSP
Sbjct: 361 NGKIYDCHELHAESLYFVHPITGIPLKLQAPLPSWASQAWQPQQQEVNSP 356

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BLAST of MELO3C004192 vs. NCBI nr
Match: KAG7021056.1 (RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 568.9 bits (1465), Expect = 3.5e-158
Identity = 306/421 (72.68%), Postives = 330/421 (78.38%), Query Frame = 0


Query: 1   MALFISLHPNFSIFPKTFAKFPFTKPFCFHRFKSVSNSMTDSSEPVTGNCPSTGENYPVP 60
           MAL +++ P FSI  K+FAKFP  KPF FHRF+ V+NSM DSS+PV      TGENYPVP
Sbjct: 6   MALVVTIQPPFSIPHKSFAKFP--KPFRFHRFRPVTNSMADSSKPV------TGENYPVP 65

Query: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120
           LSPPLPAISKNLELARAMAA SKSSLF+LSA DVIYEDEWLIAVNKPQG+YCE+VLA+VP
Sbjct: 66  LSPPLPAISKNLELARAMAACSKSSLFSLSACDVIYEDEWLIAVNKPQGVYCESVLASVP 125

Query: 121 RLLCDSANADEFNFLLHFGFSNRMVLFCIVGVSSYRERRLFSGLPVFLKENNTFTEEARA 180
           R L DSA A                     G  S                          
Sbjct: 126 RFLGDSAMA---------------------GAQS-------------------------G 185

Query: 181 RIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWK 240
            IK +LPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDH VSKSYIAFCVG++PKWK
Sbjct: 186 EIKISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSKSYIAFCVGSAPKWK 245

Query: 241 KIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRK 300
           KI VKSGHGRSKFGVWRVYAAADVGRSLPGGS+VRDMET FEVLSVNG+NT EEL + RK
Sbjct: 246 KIIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETSFEVLSVNGQNTKEELPESRK 305

Query: 301 ----EEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEG 360
               EEEETIV  +KSL+DID++KDE+LIRARPRSGRTHQIRLH QYLGIPI GDVKYEG
Sbjct: 306 DEEEEEEETIVAQSKSLIDIDARKDEILIRARPRSGRTHQIRLHSQYLGIPIVGDVKYEG 365

Query: 361 VTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQALQPQQQEVNSPQSFKTM 418
           VTEWNGK YDSHELHAESLYFVHP+TGIPLKLQAPLPSWA+QALQPQQQEVNS  S K M
Sbjct: 366 VTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWATQALQPQQQEVNSAASLKPM 372

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BLAST of MELO3C004192 vs. NCBI nr
Match: KAG6586224.1 (RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 563.1 bits (1450), Expect = 1.9e-156
Identity = 303/423 (71.63%), Postives = 327/423 (77.30%), Query Frame = 0


Query: 1   MALFISLHPNFSIFPKTFAKFPFTKPFCFHRFKSVSNSMTDSSEPVTGNCPSTGENYPVP 60
           MAL ++L   FSI  K+FAKFP  KPF FHRF+ V+NSM DSS+PV      TGENYPVP
Sbjct: 1   MALVVTLQAPFSIPNKSFAKFP--KPFRFHRFRPVTNSMADSSKPV------TGENYPVP 60

Query: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120
           LSPPLPAISKNLELARAMAA SKSSLF+LSA DV+YEDEWLIAVNKPQG+YCE+VLA+VP
Sbjct: 61  LSPPLPAISKNLELARAMAACSKSSLFSLSACDVVYEDEWLIAVNKPQGVYCESVLASVP 120

Query: 121 RLLCDSANADEFNFLLHFGFSNRMVLFCIVGVSSYRERRLFSGLPVFLKENNTFTEEARA 180
             L DSA A                                               E   
Sbjct: 121 CFLGDSAMAG----------------------------------------------EQSG 180

Query: 181 RIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWK 240
            IK +LPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDH VSKSYIAFCVG++PKWK
Sbjct: 181 EIKISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSKSYIAFCVGSAPKWK 240

Query: 241 KIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRK 300
           KI VKSGHGRSKFGVWRVYAAADVGRSLPGGS+VRDMET FEVLSVNG+NT EEL + RK
Sbjct: 241 KIIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETSFEVLSVNGQNTKEELPESRK 300

Query: 301 ------EEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIRGDVKY 360
                 EEEETIV  +KSL+DID++KDE+LIRARPRSGRTHQIRLH QYLGIPI GDVKY
Sbjct: 301 DEEEEEEEEETIVAQSKSLIDIDARKDEILIRARPRSGRTHQIRLHSQYLGIPIVGDVKY 360

Query: 361 EGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQALQPQQQEVNSPQSFK 418
           EGVTEWNGK YDSHELHAESLYFVHP+TGIPLKLQAPLPSWA+QALQPQQQEVNS  S K
Sbjct: 361 EGVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWATQALQPQQQEVNSAASLK 369

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BLAST of MELO3C004192 vs. ExPASy Swiss-Prot
Match: Q2QNM3 (RNA pseudouridine synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0560500 PE=2 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 1.7e-94
Identity = 185/351 (52.71%), Postives = 228/351 (64.96%), Query Frame = 0


Query: 52  STGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIY 111
           +TGE YPVP+SPP PA SK++EL RAM AS++S+  A S+  V++EDEWL  V+KP G+Y
Sbjct: 47  ATGE-YPVPVSPPYPAASKDVELRRAMTASARSA--AYSSAPVVFEDEWLAVVDKPAGVY 106

Query: 112 CENVLAAVPRLLCDSANADEFNFLLHFGFSNRMVLFCIVGVSSYRERRLFSGLPVFLKEN 171
           C+ +L+A+P   C +A                                            
Sbjct: 107 CDALLSALP---CSAA-------------------------------------------- 166

Query: 172 NTFTEEARARIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF 231
            T  +EA        P LHLANRLDRDTSG+MVITK +KVA KLVKAFT+H V K+Y+A 
Sbjct: 167 -TLGDEA------TKPNLHLANRLDRDTSGLMVITKCNKVAGKLVKAFTEHKVKKTYLAL 226

Query: 232 CVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSVNGKNT 291
           C+G  P W+KI + SGHGRSK G WRVYA +DVGRSLPGGS+VRDM T FEVL +NGK  
Sbjct: 227 CIGYPPAWEKIKICSGHGRSKHGAWRVYAMSDVGRSLPGGSVVRDMSTRFEVLGINGKGQ 286

Query: 292 MEELQKFRKEEEETIVVHTKSLVDIDSQKDE----VLIRARPRSGRTHQIRLHCQYLGIP 351
             E   F  +E E+I V  K+  D+ S  DE    +L+RA P+SGRTHQIRLHCQYLG P
Sbjct: 287 FREPSNFEVDETESITVQEKA-ADLTSDGDEKNSIILVRAYPQSGRTHQIRLHCQYLGFP 339

Query: 352 IRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQ 399
           IRGDVKY GV EWNG  YD H LHAESL FVHP+TG+P+  ++PLPSWA++
Sbjct: 347 IRGDVKYSGVIEWNGVDYDGHALHAESLSFVHPVTGLPVTFRSPLPSWANE 339

[Close]

BLAST of MELO3C004192 vs. ExPASy Swiss-Prot
Match: Q7XA65 (RNA pseudouridine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g56345 PE=2 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 7.1e-93
Identity = 181/345 (52.46%), Postives = 223/345 (64.64%), Query Frame = 0


Query: 56  NYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENV 115
           NYP P+S P P ISK++EL RAM ASSKSSLF L+ +D++YEDE+L+AVNKP+G+YCE V
Sbjct: 39  NYPKPISAPPPPISKDIELRRAMEASSKSSLFNLTRDDILYEDEYLMAVNKPKGVYCEAV 98

Query: 116 LAAVPRLLCDSANADEFNFLLHFGFSNRMVLFCIVGVSSYRERRLFSGLPVFLKENNTFT 175
           L + P+++ DS++                                               
Sbjct: 99  LRSAPQIVLDSSS----------------------------------------------- 158

Query: 176 EEARARIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGT 235
                       E HLANRLDRDTSGVM+ITKSHKVA+KLVKAFT+H + KSYIA C+G+
Sbjct: 159 ------------EYHLANRLDRDTSGVMIITKSHKVAAKLVKAFTEHKIRKSYIALCIGS 218

Query: 236 SPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSVNG-KNTMEE 295
           SP W+++ V SGHGRSK G WRVYAA DVGR LPGGS VRDMET FEV+SVN  KN   E
Sbjct: 219 SPNWRRVTVSSGHGRSKHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSVNSVKNESCE 278

Query: 296 LQKFRKEEEETIVVHTKSLVDIDSQKDE--VLIRARPRSGRTHQIRLHCQYLGIPIRGDV 355
           L+    +    IV   +  +      D+  V++RA PRSGRTHQIRLHCQYLGIPIRGDV
Sbjct: 279 LE----DVNHVIVAEGERELSCGGDDDDVVVVVRAFPRSGRTHQIRLHCQYLGIPIRGDV 320

Query: 356 KYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWAS 398
           KY GV EWNG+T++ HELHAE L   HP+TG  + ++APLP WA+
Sbjct: 339 KYHGVYEWNGRTFEGHELHAECLSLDHPVTGDSIVIRAPLPYWAA 320

[Close]

BLAST of MELO3C004192 vs. ExPASy Swiss-Prot
Match: Q87S65 (Ribosomal large subunit pseudouridine synthase D OS=Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633) OX=223926 GN=rluD PE=3 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 1.1e-13
Identity = 67/242 (27.69%), Postives = 105/242 (43.39%), Query Frame = 0


Query: 186 LPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVK 245
           +P   + +RLD+DT+G+MV+ K+    ++LV+A     +++ Y A  +G      K+   
Sbjct: 129 VPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVRALQKRNITREYEAIAIGRMTAGGKVDQP 188

Query: 246 SGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEET 305
            G   +K     + A A +G         +   T++ V            + FR+     
Sbjct: 189 IGRHSTK---RTLMAVAPLG---------KPAVTHYRV-----------AEHFRE----- 248

Query: 306 IVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEG--------- 365
              HT+             IR R  +GRTHQIR+H  YL  P+ GD  Y G         
Sbjct: 249 ---HTR-------------IRLRLETGRTHQIRVHMSYLQHPLLGDTAYGGRARIPTGAS 308

Query: 366 --VTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPS---WASQALQPQQQEVNSPQ 414
             +T+   + +D   LHA  L F HPITG  L+  AP+P      ++AL+   +E   P 
Sbjct: 309 QELTDMI-RGFDRQALHAVMLRFEHPITGEELEFHAPVPDDMVAMTEALRKDTEEYGLPD 325

[Close]

BLAST of MELO3C004192 vs. ExPASy Swiss-Prot
Match: Q8DEV0 (Ribosomal large subunit pseudouridine synthase D OS=Vibrio vulnificus (strain CMCP6) OX=216895 GN=rluD PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 4.2e-13
Identity = 64/241 (26.56%), Postives = 101/241 (41.91%), Query Frame = 0


Query: 186 LPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVK 245
           +P   + +RLD+DT+G+MV+ K+    ++LV+A     +++ Y A  +G      K+   
Sbjct: 129 VPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVRALQKRNITREYEAIAIGRMTAGGKVDKP 188

Query: 246 SGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEET 305
            G   +K     + A + +G         +   T+F V            + FR+     
Sbjct: 189 IGRHATK---RTLMAVSPMG---------KPAVTHFRV-----------AEHFRE----- 248

Query: 306 IVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNG--- 365
              HT+             IR R  +GRTHQIR+H  YL  P+ GD  Y G         
Sbjct: 249 ---HTR-------------IRLRLETGRTHQIRVHMSYLQHPLLGDAAYGGRARIPAGAS 308

Query: 366 -------KTYDSHELHAESLYFVHPITGIPLKLQAPLPS---WASQALQPQQQEVNSPQS 414
                  + +D   LHA  L F HPITG  ++  AP+P      ++ L+   QE   P  
Sbjct: 309 EDVTAMIRNFDRQALHAVMLRFEHPITGEEMEFHAPVPDDMVELTETLRRDAQEHGLPDE 325

[Close]

BLAST of MELO3C004192 vs. ExPASy Swiss-Prot
Match: Q68XB2 (Ribosomal large subunit pseudouridine synthase C OS=Rickettsia typhi (strain ATCC VR-144 / Wilmington) OX=257363 GN=rluC PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 9.4e-13
Identity = 66/213 (30.99%), Postives = 96/213 (45.07%), Query Frame = 0


Query: 188 ELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSG 247
           +  L +RLD++TSG+++I K++  + KL  AF +  V K Y A   G   K   I VKS 
Sbjct: 132 DFKLVHRLDKETSGLLLIAKNYLSSVKLHDAFKEKLVIKKYFAITYGRPVKNVGI-VKSN 191

Query: 248 HGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIV 307
            G+SK    R++   D+                    S NGK                 +
Sbjct: 192 IGKSK---GRMFKITDID-------------------SDNGK---------------LAI 251

Query: 308 VHTKSLVDIDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDS 367
            + K L  +++  +  LI   P +GR HQ+RLH Q LG PI GD KY          Y  
Sbjct: 252 TYYKLLKSLNN--NLFLIEFMPVTGRMHQLRLHAQLLGCPILGDDKYGNKEVMPYSKY-- 302

Query: 368 HELHAESLYFVHPITGIPLKLQAPLPSWASQAL 401
             LHA ++Y    I G  +KL+A LP + ++ L
Sbjct: 312 MFLHANNIYLSESIVGKEIKLEAKLPFYFTRRL 302

[Close]

BLAST of MELO3C004192 vs. ExPASy TrEMBL
Match: A0A0A0LJY6 (PseudoU_synth_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G033960 PE=4 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 3.8e-190
Identity = 346/417 (82.97%), Postives = 354/417 (84.89%), Query Frame = 0


Query: 1   MALFISLHPNFSIFPKTFAKFPFTKPFCFHRFKSVSNSMTDSSEPVTGNCPSTGENYPVP 60
           MALFISLHPNFSI PKTF +FPFTK FCFHRFKSVSNSMTDSSEPVTGNCPST ENYPVP
Sbjct: 1   MALFISLHPNFSISPKTFTEFPFTKSFCFHRFKSVSNSMTDSSEPVTGNCPSTAENYPVP 60

Query: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120
           LSPPLPAISKNLELARAM ASSKSSL+ALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP
Sbjct: 61  LSPPLPAISKNLELARAMVASSKSSLYALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120

Query: 121 RLLCDSANADEFNFLLHFGFSNRMVLFCIVGVSSYRERRLFSGLPVFLKENNTFTEEARA 180
           RLL DSANA                                                   
Sbjct: 121 RLLGDSANAG-------------------------------------------------- 180

Query: 181 RIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWK 240
            IKT+LPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWK
Sbjct: 181 -IKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWK 240

Query: 241 KIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRK 300
           KI+VKSGHGRSKFGVWRVYAAADVGRSLPGGS+VRDMETYFEVLSVNGKNTMEELQKFR+
Sbjct: 241 KINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNTMEELQKFRR 300

Query: 301 EEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEW 360
            EEETIVVHTKSLVDIDS KDE+LIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEW
Sbjct: 301 GEEETIVVHTKSLVDIDSHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEW 360

Query: 361 NGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQALQPQQQEVNSPQSFKTMP 418
           N K YDSHELHAESLYFVHP+TGIPLKLQAPLPSWASQALQPQQ EVNSPQSFKTMP
Sbjct: 361 NEKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQQHEVNSPQSFKTMP 366

[Close]

BLAST of MELO3C004192 vs. ExPASy TrEMBL
Match: A0A5A7TX25 (RNA pseudouridine synthase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001190 PE=4 SV=1)

HSP 1 Score: 634.4 bits (1635), Expect = 3.3e-178
Identity = 327/379 (86.28%), Postives = 327/379 (86.28%), Query Frame = 0


Query: 39  MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 98
           MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED
Sbjct: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60

Query: 99  EWLIAVNKPQGIYCENVLAAVPRLLCDSANADEFNFLLHFGFSNRMVLFCIVGVSSYRER 158
           EWLIAVNKPQGIYCENVLAAVPRLLCDSANA                             
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAG---------------------------- 120

Query: 159 RLFSGLPVFLKENNTFTEEARARIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKA 218
                                  IKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKA
Sbjct: 121 -----------------------IKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKA 180

Query: 219 FTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDME 278
           FTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDME
Sbjct: 181 FTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDME 240

Query: 279 TYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIR 338
           TYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIR
Sbjct: 241 TYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIR 300

Query: 339 LHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQ 398
           LHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQ
Sbjct: 301 LHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQ 328

Query: 399 ALQPQQQEVNSPQSFKTMP 418
           ALQPQQQEVNSPQSFKTMP
Sbjct: 361 ALQPQQQEVNSPQSFKTMP 328

[Close]

BLAST of MELO3C004192 vs. ExPASy TrEMBL
Match: A0A1S3BW87 (RNA pseudouridine synthase 1 OS=Cucumis melo OX=3656 GN=LOC103494301 PE=4 SV=1)

HSP 1 Score: 634.4 bits (1635), Expect = 3.3e-178
Identity = 327/379 (86.28%), Postives = 327/379 (86.28%), Query Frame = 0


Query: 39  MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 98
           MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED
Sbjct: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60

Query: 99  EWLIAVNKPQGIYCENVLAAVPRLLCDSANADEFNFLLHFGFSNRMVLFCIVGVSSYRER 158
           EWLIAVNKPQGIYCENVLAAVPRLLCDSANA                             
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAG---------------------------- 120

Query: 159 RLFSGLPVFLKENNTFTEEARARIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKA 218
                                  IKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKA
Sbjct: 121 -----------------------IKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKA 180

Query: 219 FTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDME 278
           FTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDME
Sbjct: 181 FTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDME 240

Query: 279 TYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIR 338
           TYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIR
Sbjct: 241 TYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIR 300

Query: 339 LHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQ 398
           LHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQ
Sbjct: 301 LHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQ 328

Query: 399 ALQPQQQEVNSPQSFKTMP 418
           ALQPQQQEVNSPQSFKTMP
Sbjct: 361 ALQPQQQEVNSPQSFKTMP 328

[Close]

BLAST of MELO3C004192 vs. ExPASy TrEMBL
Match: A0A6J1DT56 (RNA pseudouridine synthase 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023261 PE=4 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 9.7e-154
Identity = 283/376 (75.27%), Postives = 310/376 (82.45%), Query Frame = 0


Query: 39  MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 98
           M D S PVTGN P+TG+NYP+PLSPPLPAISKNLELARAMAASSKSSLFALSA DVI+ED
Sbjct: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60

Query: 99  EWLIAVNKPQGIYCENVLAAVPRLLCDSANADEFNFLLHFGFSNRMVLFCIVGVSSYRER 158
           EWLIAVNKPQG+YCE+VLA+VPRLL DSA A E         S      C+         
Sbjct: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGE--------QSGGTTTLCL--------- 120

Query: 159 RLFSGLPVFLKENNTFTEEARARIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKA 218
                                ARI+ +LPELHLANRLDRDTSGVMVITKSHKVASKLVKA
Sbjct: 121 -------------------CLARIQISLPELHLANRLDRDTSGVMVITKSHKVASKLVKA 180

Query: 219 FTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDME 278
           FTDH VSKSYIAFCVG++PKWKKI+VKSGHGRSKFGVWRVYAAADVGRSLPGGS+VRDME
Sbjct: 181 FTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDME 240

Query: 279 TYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIR 338
           TYFEVLSVNG++TM+EL + RK+EEETIVV +KSL+DID+ KDE+LIRARPRSGRTHQIR
Sbjct: 241 TYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEILIRARPRSGRTHQIR 300

Query: 339 LHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQ 398
           LHCQYLGIPIRGDVKYEGV EWNG  YDSHELHAESL+FVHP+TGIPLKLQAPLPSWASQ
Sbjct: 301 LHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGIPLKLQAPLPSWASQ 340

Query: 399 ALQPQQQEVNSPQSFK 415
           AL  QQQEV+SP + K
Sbjct: 361 ALLSQQQEVSSPSTLK 340

[Close]

BLAST of MELO3C004192 vs. ExPASy TrEMBL
Match: A0A6J1DQ12 (RNA pseudouridine synthase 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023261 PE=4 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 1.6e-151
Identity = 279/376 (74.20%), Postives = 305/376 (81.12%), Query Frame = 0


Query: 39  MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 98
           M D S PVTGN P+TG+NYP+PLSPPLPAISKNLELARAMAASSKSSLFALSA DVI+ED
Sbjct: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60

Query: 99  EWLIAVNKPQGIYCENVLAAVPRLLCDSANADEFNFLLHFGFSNRMVLFCIVGVSSYRER 158
           EWLIAVNKPQG+YCE+VLA+VPRLL DSA A                             
Sbjct: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAG---------------------------- 120

Query: 159 RLFSGLPVFLKENNTFTEEARARIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKA 218
                             E    I+ +LPELHLANRLDRDTSGVMVITKSHKVASKLVKA
Sbjct: 121 ------------------EQSGGIQISLPELHLANRLDRDTSGVMVITKSHKVASKLVKA 180

Query: 219 FTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDME 278
           FTDH VSKSYIAFCVG++PKWKKI+VKSGHGRSKFGVWRVYAAADVGRSLPGGS+VRDME
Sbjct: 181 FTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDME 240

Query: 279 TYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIR 338
           TYFEVLSVNG++TM+EL + RK+EEETIVV +KSL+DID+ KDE+LIRARPRSGRTHQIR
Sbjct: 241 TYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEILIRARPRSGRTHQIR 300

Query: 339 LHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQ 398
           LHCQYLGIPIRGDVKYEGV EWNG  YDSHELHAESL+FVHP+TGIPLKLQAPLPSWASQ
Sbjct: 301 LHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGIPLKLQAPLPSWASQ 330

Query: 399 ALQPQQQEVNSPQSFK 415
           AL  QQQEV+SP + K
Sbjct: 361 ALLSQQQEVSSPSTLK 330

[Close]

BLAST of MELO3C004192 vs. TAIR 10
Match: AT1G56345.1 (Pseudouridine synthase family protein )

HSP 1 Score: 342.4 bits (877), Expect = 5.0e-94
Identity = 181/345 (52.46%), Postives = 223/345 (64.64%), Query Frame = 0


Query: 56  NYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENV 115
           NYP P+S P P ISK++EL RAM ASSKSSLF L+ +D++YEDE+L+AVNKP+G+YCE V
Sbjct: 39  NYPKPISAPPPPISKDIELRRAMEASSKSSLFNLTRDDILYEDEYLMAVNKPKGVYCEAV 98

Query: 116 LAAVPRLLCDSANADEFNFLLHFGFSNRMVLFCIVGVSSYRERRLFSGLPVFLKENNTFT 175
           L + P+++ DS++                                               
Sbjct: 99  LRSAPQIVLDSSS----------------------------------------------- 158

Query: 176 EEARARIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGT 235
                       E HLANRLDRDTSGVM+ITKSHKVA+KLVKAFT+H + KSYIA C+G+
Sbjct: 159 ------------EYHLANRLDRDTSGVMIITKSHKVAAKLVKAFTEHKIRKSYIALCIGS 218

Query: 236 SPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSVNG-KNTMEE 295
           SP W+++ V SGHGRSK G WRVYAA DVGR LPGGS VRDMET FEV+SVN  KN   E
Sbjct: 219 SPNWRRVTVSSGHGRSKHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSVNSVKNESCE 278

Query: 296 LQKFRKEEEETIVVHTKSLVDIDSQKDE--VLIRARPRSGRTHQIRLHCQYLGIPIRGDV 355
           L+    +    IV   +  +      D+  V++RA PRSGRTHQIRLHCQYLGIPIRGDV
Sbjct: 279 LE----DVNHVIVAEGERELSCGGDDDDVVVVVRAFPRSGRTHQIRLHCQYLGIPIRGDV 320

Query: 356 KYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWAS 398
           KY GV EWNG+T++ HELHAE L   HP+TG  + ++APLP WA+
Sbjct: 339 KYHGVYEWNGRTFEGHELHAECLSLDHPVTGDSIVIRAPLPYWAA 320

[Close]

BLAST of MELO3C004192 vs. TAIR 10
Match: AT1G76050.2 (Pseudouridine synthase family protein )

HSP 1 Score: 73.6 bits (179), Expect = 4.3e-13
Identity = 77/329 (23.40%), Postives = 128/329 (38.91%), Query Frame = 0


Query: 93  DVIYEDEWLIAVNKPQGIYCENVLAAVPRLLCDSANADEFNFLLHFGFSNRMVLFCIVGV 152
           D++YED+ ++ VNKP              ++   A  +    L++ G  +   L C+   
Sbjct: 149 DIVYEDKHVLVVNKP------------AHMVVHPAPGNPTGTLVN-GILHHCSLPCVDYS 208

Query: 153 SSYRERRLFSGLPVFLKENNTFTEEARARIKTNLPELHLA-------NRLDRDTSGVMVI 212
           +S  +            +  TF+++      T  P  + A       +RLD+ T+G++V+
Sbjct: 209 NSEEDDD---------SDEETFSDDEE---MTTSPSSYAASVRPGIVHRLDKGTTGLLVV 268

Query: 213 TKSHKVASKLVKAFTDHTVSKSYIAFCVGT-SPKWKKIHVKSGHGRSKFGVWRVYAAADV 272
            K     + L + F  HT+ + Y++   G  SP   +I +  G   S     R+  AA  
Sbjct: 269 AKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRDSSN----RIRMAA-- 328

Query: 273 GRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVL 332
              +PGG              V G        +++             +++  +     L
Sbjct: 329 ---IPGG--------------VRGGRARHAASRYK-------------VIETFAGGGSAL 388

Query: 333 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEG-------VTEWNGKTYDSHE-------- 392
           +  R  +GRTHQIR H +Y+G+P+ GD  Y G       + +      D  E        
Sbjct: 389 VEWRLETGRTHQIRAHAKYMGVPLLGDEVYGGTKSMALSLLQKRVSRSDQEEIIELISRM 416

Query: 393 ----LHAESLYFVHPITGIPLKLQAPLPS 395
               LHA  L F HP TG  +K   P PS
Sbjct: 449 DRPCLHAIVLGFTHPCTGEIVKFSCPPPS 416

[Close]

BLAST of MELO3C004192 vs. TAIR 10
Match: AT5G51140.1 (Pseudouridine synthase family protein )

HSP 1 Score: 52.0 bits (123), Expect = 1.4e-06
Identity = 52/196 (26.53%), Postives = 75/196 (38.27%), Query Frame = 0


Query: 185 NLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHV 244
           +L  L   +RLDR  SG+++I ++   A    +      V K YIA  +G  P+ + I  
Sbjct: 176 DLGTLFPIHRLDRLVSGLLIIARTAAKADFFRQQIEGGMVKKRYIAKVIGVFPEDEMI-- 235

Query: 245 KSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEE 304
                        V A  +   S  G S   D  +  +   V GK    +  +       
Sbjct: 236 -------------VDANINYNGS-EGRSTAEDANSSGDDKKVKGKPACTKFTR------- 295

Query: 305 TIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKT 364
                      ID+     L+   P +GRTHQIR+H QY G PI  D  Y      N +T
Sbjct: 296 -----------IDTNGTHSLVSCEPVTGRTHQIRVHLQYTGHPIANDPLYLNQHIDNLET 337

Query: 365 YDSHELHAESLYFVHP 381
           Y +  + A     V P
Sbjct: 356 YIAKRIDAGERKIVSP 337

[Close]

BLAST of MELO3C004192 vs. TAIR 10
Match: AT5G51140.2 (Pseudouridine synthase family protein )

HSP 1 Score: 52.0 bits (123), Expect = 1.4e-06
Identity = 52/196 (26.53%), Postives = 75/196 (38.27%), Query Frame = 0


Query: 185 NLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHV 244
           +L  L   +RLDR  SG+++I ++   A    +      V K YIA  +G  P+ + I  
Sbjct: 176 DLGTLFPIHRLDRLVSGLLIIARTAAKADFFRQQIEGGMVKKRYIAKVIGVFPEDEMI-- 235

Query: 245 KSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEE 304
                        V A  +   S  G S   D  +  +   V GK    +  +       
Sbjct: 236 -------------VDANINYNGS-EGRSTAEDANSSGDDKKVKGKPACTKFTR------- 295

Query: 305 TIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKT 364
                      ID+     L+   P +GRTHQIR+H QY G PI  D  Y      N +T
Sbjct: 296 -----------IDTNGTHSLVSCEPVTGRTHQIRVHLQYTGHPIANDPLYLNQHIDNLET 337

Query: 365 YDSHELHAESLYFVHP 381
           Y +  + A     V P
Sbjct: 356 YIAKRIDAGERKIVSP 337

[Close]

BLAST of MELO3C004192 vs. TAIR 10
Match: AT3G52260.2 (Pseudouridine synthase family protein )

HSP 1 Score: 43.9 bits (102), Expect = 3.7e-04
Identity = 44/166 (26.51%), Postives = 70/166 (42.17%), Query Frame = 0


Query: 193 NRLDRDTSGVMVITKSHKVASKLVKAFTDHT--VSKSYIAFCVGTSPKWKKIHVKSGHGR 252
           +RL R TSG+++  K+    +KL   F + T  V    +    GT  K  KI+     G 
Sbjct: 155 HRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYRALADG- 214

Query: 253 SKFGVWRVYAAADVGRSLPGGSIVR--DMETYFEVLSVNGKNTMEELQKFRKEEEETIVV 312
                  +    +V    P G +VR   +     V S  GK    ++             
Sbjct: 215 -------IVEEDEVVIKQPIG-VVRYPGVAQGLYVASPEGKPAFSKV------------- 274

Query: 313 HTKSLVDIDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIRGDVKY 355
               +++ D +K+  L++   +SGR HQIR+H  Y+G P+ GD  Y
Sbjct: 275 ---FVLERDREKNCSLVKVEIQSGRPHQIRIHLAYMGHPLVGDPLY 295

The following BLAST results are available for this feature:

BLAST of MELO3C004192 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004144814.3	7.8e-190	82.97	RNA pseudouridine synthase 1 [Cucumis sativus] >KGN60992.1 hypothetical protein ...	[more]
XP_008453651.1	6.8e-178	86.28	PREDICTED: RNA pseudouridine synthase 1 [Cucumis melo] >KAA0048062.1 RNA pseudou...	[more]
XP_038889468.1	1.8e-170	77.80	RNA pseudouridine synthase 1 [Benincasa hispida]	[more]
KAG7021056.1	3.5e-158	72.68	RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. argyrosperm...	[more]
KAG6586224.1	1.9e-156	71.63	RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of MELO3C004192 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2QNM3	1.7e-94	52.71	RNA pseudouridine synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g05...	[more]
Q7XA65	7.1e-93	52.46	RNA pseudouridine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g56345 PE=2 S...	[more]
Q87S65	1.1e-13	27.69	Ribosomal large subunit pseudouridine synthase D OS=Vibrio parahaemolyticus sero...	[more]
Q8DEV0	4.2e-13	26.56	Ribosomal large subunit pseudouridine synthase D OS=Vibrio vulnificus (strain CM...	[more]
Q68XB2	9.4e-13	30.99	Ribosomal large subunit pseudouridine synthase C OS=Rickettsia typhi (strain ATC...	[more]

BLAST of MELO3C004192 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LJY6	3.8e-190	82.97	PseudoU_synth_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G03...	[more]
A0A5A7TX25	3.3e-178	86.28	RNA pseudouridine synthase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3BW87	3.3e-178	86.28	RNA pseudouridine synthase 1 OS=Cucumis melo OX=3656 GN=LOC103494301 PE=4 SV=1	[more]
A0A6J1DT56	9.7e-154	75.27	RNA pseudouridine synthase 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1DQ12	1.6e-151	74.20	RNA pseudouridine synthase 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111...	[more]

BLAST of MELO3C004192 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56345.1	5.0e-94	52.46	Pseudouridine synthase family protein	[more]
AT1G76050.2	4.3e-13	23.40	Pseudouridine synthase family protein	[more]
AT5G51140.1	1.4e-06	26.53	Pseudouridine synthase family protein	[more]
AT5G51140.2	1.4e-06	26.53	Pseudouridine synthase family protein	[more]
AT3G52260.2	3.7e-04	26.51	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006145	Pseudouridine synthase, RsuA/RluA-like	PFAM	PF00849	PseudoU_synth_2	coord: 101..342 e-value: 4.0E-24 score: 85.5
IPR006145	Pseudouridine synthase, RsuA/RluA-like	CDD	cd02869	PseudoU_synth_RluA_like	coord: 178..377 e-value: 6.60692E-37 score: 130.92
None	No IPR available	GENE3D	3.30.2350.10	Pseudouridine synthase	coord: 89..393 e-value: 9.1E-44 score: 151.6
None	No IPR available	PANTHER	PTHR21600	MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE	coord: 45..404
None	No IPR available	PANTHER	PTHR21600:SF47	RNA PSEUDOURIDINE SYNTHASE 1	coord: 45..404
IPR006224	Pseudouridine synthase, RluC/RluD, conserved site	PROSITE	PS01129	PSI_RLU	coord: 192..206
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 92..402

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C004192.1	MELO3C004192.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding