MELO3C003855 (gene) Melon (DHL92) v4

Overview


Name	MELO3C003855
Type	gene
Organism	Cucumis melo cv. DHL92 (Melon (DHL92) v4)
Description	Cytoplasmic tRNA 2-thiolation protein 1-like
Location	chr04: 5424389 .. 5424674 (-)
RNA-Seq Expression	MELO3C003855
Synteny	MELO3C003855

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTTTTTTTTTCTCCTTAAGGCTCTTGACAGAGGTGTTGCATTGTTGAAAGTAGTCAAGCTAGCCACAGGGCACAATGCAATTGATATGGTTGGAACAATTCTATTAAATATATTACGAGGTGATATTCCAAGGTATTTTAATAGTAAGACTTTTTATTTCTGTCAATTACTTTTGTCCCTCATTTGGGTTGTATTAGACTGAAGTGATGCTTTGTATTTGTAGATTGAGTAGATGTACTGCATTAATTACCGACGAAGATGGACCAAATCCAAGATGCTAA

mRNA sequence

ATGGATTTTTTTTTTCTCCTTAAGGCTCTTGACAGAGGTGTTGCATTGTTGAAAGTAGTCAAGCTAGCCACAGGGCACAATGCAATTGATATGGTTGGAACAATTCTATTAAATATATTACGAGGTGATATTCCAAGATTGAGTAGATGTACTGCATTAATTACCGACGAAGATGGACCAAATCCAAGATGCTAA

Coding sequence (CDS)

ATGGATTTTTTTTTTCTCCTTAAGGCTCTTGACAGAGGTGTTGCATTGTTGAAAGTAGTCAAGCTAGCCACAGGGCACAATGCAATTGATATGGTTGGAACAATTCTATTAAATATATTACGAGGTGATATTCCAAGATTGAGTAGATGTACTGCATTAATTACCGACGAAGATGGACCAAATCCAAGATGCTAA

Protein sequence

MDFFFLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC

Homology

[Close]

BLAST of MELO3C003855 vs. NCBI nr
Match: TYJ97980.1 (cytoplasmic tRNA 2-thiolation protein 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 129.8 bits (325), Expect = 8.4e-27
Identity = 64/64 (100.00%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 1   MDFFFLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGP 60
           MDFFFLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGP
Sbjct: 108 MDFFFLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGP 167

Query: 61  NPRC 65
           NPRC
Sbjct: 168 NPRC 171

[Close]

BLAST of MELO3C003855 vs. NCBI nr
Match: TYK20555.1 (cytoplasmic tRNA 2-thiolation protein 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 110.2 bits (274), Expect = 6.9e-21
Identity = 56/61 (91.80%), Postives = 57/61 (93.44%), Query Frame = 0


Query: 4   FFLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPR 63
           FFLLKALDR VALLKVV+LATGHNAIDMVGTILLNILRGDI RLSRCT LITDEDGP PR
Sbjct: 94  FFLLKALDRVVALLKVVRLATGHNAIDMVGTILLNILRGDIARLSRCTKLITDEDGPIPR 153

Query: 64  C 65
           C
Sbjct: 154 C 154

[Close]

BLAST of MELO3C003855 vs. NCBI nr
Match: KAA0038049.1 (cytoplasmic tRNA 2-thiolation protein 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 110.2 bits (274), Expect = 6.9e-21
Identity = 56/61 (91.80%), Postives = 57/61 (93.44%), Query Frame = 0


Query: 4   FFLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPR 63
           FFLLKALDR VALLKVV+LATGHNAIDMVGTILLNILRGDI RLSRCT LITDEDGP PR
Sbjct: 94  FFLLKALDRVVALLKVVRLATGHNAIDMVGTILLNILRGDIARLSRCTKLITDEDGPIPR 153

Query: 64  C 65
           C
Sbjct: 154 C 154

[Close]

BLAST of MELO3C003855 vs. NCBI nr
Match: KAA0041648.1 (cytoplasmic tRNA 2-thiolation protein 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 107.8 bits (268), Expect = 3.4e-20
Identity = 52/55 (94.55%), Postives = 53/55 (96.36%), Query Frame = 0


Query: 10  LDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC 65
           +  GVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC
Sbjct: 88  ISSGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC 142

[Close]

BLAST of MELO3C003855 vs. NCBI nr
Match: TYK25841.1 (cytoplasmic tRNA 2-thiolation protein 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 102.8 bits (255), Expect = 1.1e-18
Identity = 52/56 (92.86%), Postives = 52/56 (92.86%), Query Frame = 0


Query: 9   ALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC 65
           ALDRGVALLKVVKLA GHNAIDMVGTILLNILRGDI RLSRCTALIT EDGP PRC
Sbjct: 116 ALDRGVALLKVVKLAMGHNAIDMVGTILLNILRGDIARLSRCTALITAEDGPIPRC 171

[Close]

BLAST of MELO3C003855 vs. ExPASy Swiss-Prot
Match: O64862 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Arabidopsis thaliana OX=3702 GN=NCS6 PE=2 SV=2)

HSP 1 Score: 87.4 bits (215), Expect = 6.3e-17
Identity = 43/60 (71.67%), Postives = 48/60 (80.00%), Query Frame = 0


Query: 5   FLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC 64
           F  +ALDRG ALLKV KL TGHNA D+  T+LLNILRGDI RLSRCT++ T EDGP PRC
Sbjct: 155 FRRQALDRGAALLKVEKLVTGHNADDIAETVLLNILRGDIARLSRCTSITTGEDGPIPRC 214

[Close]

BLAST of MELO3C003855 vs. ExPASy Swiss-Prot
Match: Q6Z6G6 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=NCS6 PE=2 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 3.1e-16
Identity = 42/60 (70.00%), Postives = 47/60 (78.33%), Query Frame = 0


Query: 5   FLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC 64
           F  +ALDRG ALLKV K+ TGHNA D+  T+LLNILRGDI RLSRCT + T EDGP PRC
Sbjct: 161 FRRQALDRGAALLKVDKIVTGHNADDIAETVLLNILRGDIARLSRCTFITTGEDGPIPRC 220

[Close]

BLAST of MELO3C003855 vs. ExPASy Swiss-Prot
Match: A8JF71 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Chlamydomonas reinhardtii OX=3055 GN=NCS6 PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 2.1e-12
Identity = 35/59 (59.32%), Postives = 42/59 (71.19%), Query Frame = 0


Query: 5   FLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPR 64
           F  +ALDRG  L+   K+ATGHNA D+  T+LLNILRGD+PRL RC  +IT ED   PR
Sbjct: 147 FRRQALDRGAVLMGANKIATGHNADDVAETVLLNILRGDVPRLGRCANIITGEDSSLPR 205

[Close]

BLAST of MELO3C003855 vs. ExPASy Swiss-Prot
Match: Q94480 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Dictyostelium discoideum OX=44689 GN=ctu1 PE=2 SV=2)

HSP 1 Score: 71.2 bits (173), Expect = 4.7e-12
Identity = 33/59 (55.93%), Postives = 42/59 (71.19%), Query Frame = 0


Query: 5   FLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPR 64
           F  +ALDRG  +LK  K+ TGHNA D+  T+L+N+LRGDIPRL RC  +IT  +G  PR
Sbjct: 154 FRRQALDRGAVMLKANKIVTGHNADDIAETVLMNLLRGDIPRLQRCVNIITGSEGALPR 212

[Close]

BLAST of MELO3C003855 vs. ExPASy Swiss-Prot
Match: Q05AW7 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Xenopus laevis OX=8355 GN=ctu1 PE=2 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 6.1e-12
Identity = 33/60 (55.00%), Postives = 42/60 (70.00%), Query Frame = 0


Query: 5   FLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC 64
           F  +ALDRG  +L + K+ TGHNA D+  T+L+N LRGDI RL RCTA+ T  +G  PRC
Sbjct: 146 FRRQALDRGAMMLGINKICTGHNADDIAETVLMNFLRGDIARLRRCTAITTGSEGAIPRC 205

[Close]

BLAST of MELO3C003855 vs. ExPASy TrEMBL
Match: A0A5D3BDQ2 (Cytoplasmic tRNA 2-thiolation protein 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold234G00920 PE=3 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 4.1e-27
Identity = 64/64 (100.00%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 1   MDFFFLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGP 60
           MDFFFLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGP
Sbjct: 108 MDFFFLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGP 167

Query: 61  NPRC 65
           NPRC
Sbjct: 168 NPRC 171

[Close]

BLAST of MELO3C003855 vs. ExPASy TrEMBL
Match: A0A5D3DAG6 (Cytoplasmic tRNA 2-thiolation protein 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G001430 PE=3 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 3.3e-21
Identity = 56/61 (91.80%), Postives = 57/61 (93.44%), Query Frame = 0


Query: 4   FFLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPR 63
           FFLLKALDR VALLKVV+LATGHNAIDMVGTILLNILRGDI RLSRCT LITDEDGP PR
Sbjct: 94  FFLLKALDRVVALLKVVRLATGHNAIDMVGTILLNILRGDIARLSRCTKLITDEDGPIPR 153

Query: 64  C 65
           C
Sbjct: 154 C 154

[Close]

BLAST of MELO3C003855 vs. ExPASy TrEMBL
Match: A0A5A7T9N6 (Cytoplasmic tRNA 2-thiolation protein 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G002520 PE=3 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 3.3e-21
Identity = 56/61 (91.80%), Postives = 57/61 (93.44%), Query Frame = 0


Query: 4   FFLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPR 63
           FFLLKALDR VALLKVV+LATGHNAIDMVGTILLNILRGDI RLSRCT LITDEDGP PR
Sbjct: 94  FFLLKALDRVVALLKVVRLATGHNAIDMVGTILLNILRGDIARLSRCTKLITDEDGPIPR 153

Query: 64  C 65
           C
Sbjct: 154 C 154

[Close]

BLAST of MELO3C003855 vs. ExPASy TrEMBL
Match: A0A5A7TFF5 (Cytoplasmic tRNA 2-thiolation protein 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold93G001250 PE=3 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.7e-20
Identity = 52/55 (94.55%), Postives = 53/55 (96.36%), Query Frame = 0


Query: 10  LDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC 65
           +  GVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC
Sbjct: 88  ISSGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC 142

[Close]

BLAST of MELO3C003855 vs. ExPASy TrEMBL
Match: A0A5D3DQ85 (Cytoplasmic tRNA 2-thiolation protein 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold436G00490 PE=4 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 5.3e-19
Identity = 52/56 (92.86%), Postives = 52/56 (92.86%), Query Frame = 0


Query: 9   ALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC 65
           ALDRGVALLKVVKLA GHNAIDMVGTILLNILRGDI RLSRCTALIT EDGP PRC
Sbjct: 116 ALDRGVALLKVVKLAMGHNAIDMVGTILLNILRGDIARLSRCTALITAEDGPIPRC 171

[Close]

BLAST of MELO3C003855 vs. TAIR 10
Match: AT2G44270.1 (repressor of lrx1 )

HSP 1 Score: 87.4 bits (215), Expect = 4.5e-18
Identity = 43/60 (71.67%), Postives = 48/60 (80.00%), Query Frame = 0


Query: 5   FLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC 64
           F  +ALDRG ALLKV KL TGHNA D+  T+LLNILRGDI RLSRCT++ T EDGP PRC
Sbjct: 155 FRRQALDRGAALLKVEKLVTGHNADDIAETVLLNILRGDIARLSRCTSITTGEDGPIPRC 214

[Close]

BLAST of MELO3C003855 vs. TAIR 10
Match: AT2G44270.2 (repressor of lrx1 )

HSP 1 Score: 87.4 bits (215), Expect = 4.5e-18
Identity = 43/60 (71.67%), Postives = 48/60 (80.00%), Query Frame = 0


Query: 5   FLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC 64
           F  +ALDRG ALLKV KL TGHNA D+  T+LLNILRGDI RLSRCT++ T EDGP PRC
Sbjct: 165 FRRQALDRGAALLKVEKLVTGHNADDIAETVLLNILRGDIARLSRCTSITTGEDGPIPRC 224

[Close]

BLAST of MELO3C003855 vs. TAIR 10
Match: AT1G76170.1 (2-thiocytidine tRNA biosynthesis protein, TtcA )

HSP 1 Score: 85.9 bits (211), Expect = 1.3e-17
Identity = 42/60 (70.00%), Postives = 48/60 (80.00%), Query Frame = 0


Query: 5   FLLKALDRGVALLKVVKLATGHNAIDMVGTILLNILRGDIPRLSRCTALITDEDGPNPRC 64
           F  +ALDRG ALLKV KL TGHNA D+  T++LNILRGDI RLSRCT++ T EDGP PRC
Sbjct: 133 FRRQALDRGAALLKVDKLVTGHNADDIAETVVLNILRGDIARLSRCTSITTGEDGPIPRC 192

The following BLAST results are available for this feature:

BLAST of MELO3C003855 vs. NCBI nr


Match Name	E-value	Identity	Description
TYJ97980.1	8.4e-27	100.00	cytoplasmic tRNA 2-thiolation protein 1-like [Cucumis melo var. makuwa]	[more]
TYK20555.1	6.9e-21	91.80	cytoplasmic tRNA 2-thiolation protein 1-like [Cucumis melo var. makuwa]	[more]
KAA0038049.1	6.9e-21	91.80	cytoplasmic tRNA 2-thiolation protein 1-like [Cucumis melo var. makuwa]	[more]
KAA0041648.1	3.4e-20	94.55	cytoplasmic tRNA 2-thiolation protein 1-like [Cucumis melo var. makuwa]	[more]
TYK25841.1	1.1e-18	92.86	cytoplasmic tRNA 2-thiolation protein 1-like [Cucumis melo var. makuwa]	[more]

BLAST of MELO3C003855 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O64862	6.3e-17	71.67	Cytoplasmic tRNA 2-thiolation protein 1 OS=Arabidopsis thaliana OX=3702 GN=NCS6 ...	[more]
Q6Z6G6	3.1e-16	70.00	Cytoplasmic tRNA 2-thiolation protein 1 OS=Oryza sativa subsp. japonica OX=39947...	[more]
A8JF71	2.1e-12	59.32	Cytoplasmic tRNA 2-thiolation protein 1 OS=Chlamydomonas reinhardtii OX=3055 GN=...	[more]
Q94480	4.7e-12	55.93	Cytoplasmic tRNA 2-thiolation protein 1 OS=Dictyostelium discoideum OX=44689 GN=...	[more]
Q05AW7	6.1e-12	55.00	Cytoplasmic tRNA 2-thiolation protein 1 OS=Xenopus laevis OX=8355 GN=ctu1 PE=2 S...	[more]

BLAST of MELO3C003855 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BDQ2	4.1e-27	100.00	Cytoplasmic tRNA 2-thiolation protein 1-like OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A5D3DAG6	3.3e-21	91.80	Cytoplasmic tRNA 2-thiolation protein 1-like OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A5A7T9N6	3.3e-21	91.80	Cytoplasmic tRNA 2-thiolation protein 1-like OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A5A7TFF5	1.7e-20	94.55	Cytoplasmic tRNA 2-thiolation protein 1-like OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A5D3DQ85	5.3e-19	92.86	Cytoplasmic tRNA 2-thiolation protein 1-like OS=Cucumis melo var. makuwa OX=1194...	[more]

BLAST of MELO3C003855 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G44270.1	4.5e-18	71.67	repressor of lrx1	[more]
AT2G44270.2	4.5e-18	71.67	repressor of lrx1	[more]
AT1G76170.1	1.3e-17	70.00	2-thiocytidine tRNA biosynthesis protein, TtcA	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 3..64 e-value: 1.3E-8 score: 36.2
IPR000541	Cytoplasmic tRNA 2-thiolation protein 1	PANTHER	PTHR11807:SF12	CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 1	coord: 8..63
None	No IPR available	PANTHER	PTHR11807	ATPASES OF THE PP SUPERFAMILY-RELATED	coord: 8..63
None	No IPR available	SUPERFAMILY	52402	Adenine nucleotide alpha hydrolases-like	coord: 15..55

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C003855.1	MELO3C003855.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0034227	tRNA thio-modification
biological_process	GO:0002098	tRNA wobble uridine modification
molecular_function	GO:0000049	tRNA binding