MELO3C003385.jh1 (gene) Melon (Harukei-3) v1.41

Overview
NameMELO3C003385.jh1
Typegene
OrganismCucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
Descriptionbeta-amyrin 11-oxidase-like
Locationchr04: 677325 .. 680779 (-)
RNA-Seq ExpressionMELO3C003385.jh1
SyntenyMELO3C003385.jh1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDSstart_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCAATTTTTTGTCATTATTTATTGATTAAAATAATATCATTTAGAAAAATCAACAGAAGAAGACAAAACTAGAAAGTTGAATGAATTGCTAAATTGATTTGACTTACATTACTAGGCACACACTACAAAAAGGCTTTTCATCAATGCAGGTTTGGAGCTTCACAAGCTATTCGAGGATGGAAATGATAAGTGTTTGTGTAATTATTGGAGTTGTCTTAGGAGTTTCTTTGTTGGTGTTTAGGCGACTCAATGAATTATGGTATTCGGTTAAGTTAGGAGGAAGAGCTTACAAGTCTCTTCCTCCTGGTGACTTGGGATGGCCTGTTATTGGCTCTTCCTTCTCCTCTTACAAAACATTCATGGTTCAAGAAGATCCAATCTCTTTTATTCAATCTCTTCACTCAAGGTATCTCTTTCAATCTCTTTCTTCTTAAATCGGAAGATTCTAAGCCGATAATCATTTCGATCGGAAGAAGCAATTTTGATCTTATTTCTTAATTGTGGACTTCTTTATGCAGATATGGAGAAGGGGGAATATATAAAACTCACCTTTATGGAAAGCCAACAGTGATTGCCACAGATCCAGAAATATGTCGACGGATATACTTAGACGAAGCAAAGTTCAAACAACATAATCCAGAGTCGGTGAAAATCCTAGAAGGATCAAGTGGTGATTTCTCAAAGATGGATCACAAAATTGCATATAAAGTAGTGGCAACACCCATGAATGGGTCTGAGGTATTATCTAACTATGTGGATTTCATTGAGGAAGTAATAGCGAAAGGGTTGGAGGAATGGAGTAGCATGTTGAGAGAGCCTATTAAATTGTTGGATGAAATTGGAAGTCTCTTTTTCAAAGTCATTACAAAAGTTTTCTTAGGGTCTCGACTTGATGCCAAAACCATGGTAGAGCTTCATGCATTGTACAAAGAATTGAGTTTTGCAATGGTCATGTCCACTTTCCCTTATGACTTCCCTGGATTCACTTTTCACCAAGCATTCAAGGTACACAATTTTTTGTTTTTTCTTCTATATATATATCAGTCCCATCTTTTCTTGACACCATCTTTGTTTTATCAGCACTCAACATCGTCTTTTTCTGTACGACATTGTTGGTAGGCAAGAAAGAAGATAGAGAATATCATTCAAGGTGTGGTTGAGGAGAAAAGATCAAGAAGATTTGAAAATGATAAAACAAATGAAGTGCAATGTCAAGTGGACAAAATGGTTGTAGCCATTGATGAGAATGGGGAAAAATTATATAATAACAATCTCATCAGAGATTTGCTTCTTCGGGTATTATTCGCTGGTCATAGTACTCCTGCCATTGCTGCTTTTTGGGCTCTGCTACATATCTCACAAAACCCTCATGTTTTCCAGAAGGCCAAGGTTAGGTTATTATATATTTTCATCCCTATACTTTCGATCAAAGGTTTAATTAATTCTAAGATGTTCAATAAAATGAGTTAATTATTATTAATTAATTATTTACAGGAAGAGCAAGAGTCGATCATAAGACAAAGACCATCTAATAAAAAAGGCTTAACTCTTAACGAAATCAAACAAATGAAATATCTCACAAAGGTAATTTCTTTCATTTGAAGTTTACCTAATTATATCGACGATAAGCTCTTATTTTCTTGACTTTCTAAATGATTATAAACATTTTCTTTTATACTTACACTTAGAATGTCAATTCAAACCCACCTTTAATTCATCCCAATTATAACCAACCTTATTATAACTATAAATGGTAGAATCATATCACTTCTATATCAATAATTCTTAATAGAAGCCTATTAAATGATGATGGCCTTGTATTACGATAAATTTCTATGATGGACAAAATTATATCACAAATTTAAGTTGTGTCATCGTAGCTAACAAATAATCCCTTCTGTCTATTAACAAAGTTGTCCATTTCTAATTGTTCACAAAAGACTAAACATGATATAAAAGATATAATGTTTAATGGAATGATTGAAACTTATAAAAAATTAGTGTTTTGTTAATGATAAACCATATCACCAATATGATCTACTAGTCGTAAGTAGAAATACATTAGATAGAATCTTATTCAGTAAGATAATTTCATAATTTTCAAAATTGATTCTTCAATGTCAAAAATTATTTTTAATTGTGGTAGATTTTAATTGTTGTTGTCTTATATTGTTTTAGGTCATTAACGAAGTTTTGAGAAGAAATACAATTACTCCCACAAATTTTCGTGAGGCAAAAACAGATGTAAACATCAATGGTAAGTGTTTTGTTATTATTATTATTATTATTTTATGGCATATAAGTATTTAAAAATTCATTCCCATTTTATATTATACAATTGGTTCAAATTTGACATATTTGTTTTTTTAGGGAAATTAAAACTTTTATTAAAAAGCCACAAGGGAAGAAGAATGAAAATTTTCCTTTAAGGAAGCATGAGAAATTATTATTATTATTAATACATAATATTTGTTACTAATTATGTATTTAGATTGAAATTATTATTTTGTTGATGATAAATAATTAACTAATTTATTTATTTTTTTCTATTGTTTTGTTTTTGTTTTCTGTTTTAGGTTATTTTATTCCAAAAGGATGGACGATACAAATTTGGAACATAGCTGTTCATATGGACCCTCAAATTTATTCTAATCCACAAGAATTTAATCCTTCAAGATGGGAAGTATGTTTTATGATTATTTTAATCAACCATTAAAATAAACTATTGTGCTTTTGAGTTTGATTCGTTTTAGTTATTAAACTTTTAAAAAGTAACCATTTAATTTCCTCCTTTTCATTTTAAGGAGAAAATAACGTATAAATCTAAATAGTAACTTTTTCAAAAATACAAGAACCCAAATTGAAGTTGAGACCAAAATGAATAAAAACCAAAGGAGCGTTTGCAAAAATAGCAAAAGAATTTATGATAACAGTGCTTATGTCACTGCATTTTCTAAATTGCAAAAATATCAAATTTAAAAGTGGATAGTTCTTTGATAGCCCATGATAACCGTATATCTAATTACTTGTTATGAACTGTTTTTTTCCTAAAGATTTATTATTTGATACACAAGCACCACACATGCACACACGAGCATGGGGTGCACATCCACGCACCTTGACACAAGCAAAATGTTTTCATATTAATGGATTCGATTTTGACCCGATTCACCTTTGTTCCTCGTTAGTTTAGCACACGCATACATGTAAAATATCTTAAGAATAGTGTTGCTGAATGAGAATCTTTTCTTAAGAAGAAAAGTAGAGAGAAATAATAACAAAATTAAGAATATTAATGTTAACAGTATTTGTAAAATGTGATGGGCAGAATTATACACCAAAGCCAGGAACCTTCATCCCTTTTGGACTTGGAAGTAGGTTTTGTCCTGGAAGTGAACTTGCAAGGCTTGAGATTACTATTCTACTTCATCATTTCATCCTCAATTACAAGTAT

mRNA sequence

CCCAATTTTTTGTCATTATTTATTGATTAAAATAATATCATTTAGAAAAATCAACAGAAGAAGACAAAACTAGAAAGTTGAATGAATTGCTAAATTGATTTGACTTACATTACTAGGCACACACTACAAAAAGGCTTTTCATCAATGCAGGTTTGGAGCTTCACAAGCTATTCGAGGATGGAAATGATAAGTGTTTGTGTAATTATTGGAGTTGTCTTAGGAGTTTCTTTGTTGGTGTTTAGGCGACTCAATGAATTATGGTATTCGGTTAAGTTAGGAGGAAGAGCTTACAAGTCTCTTCCTCCTGGTGACTTGGGATGGCCTGTTATTGGCTCTTCCTTCTCCTCTTACAAAACATTCATGGTTCAAGAAGATCCAATCTCTTTTATTCAATCTCTTCACTCAAGATATGGAGAAGGGGGAATATATAAAACTCACCTTTATGGAAAGCCAACAGTGATTGCCACAGATCCAGAAATATGTCGACGGATATACTTAGACGAAGCAAAGTTCAAACAACATAATCCAGAGTCGGTGAAAATCCTAGAAGGATCAAGTGGTGATTTCTCAAAGATGGATCACAAAATTGCATATAAAGTAGTGGCAACACCCATGAATGGGTCTGAGGTATTATCTAACTATGTGGATTTCATTGAGGAAGTAATAGCGAAAGGGTTGGAGGAATGGAGTAGCATGTTGAGAGAGCCTATTAAATTGTTGGATGAAATTGGAAGTCTCTTTTTCAAAGTCATTACAAAAGTTTTCTTAGGGTCTCGACTTGATGCCAAAACCATGGTAGAGCTTCATGCATTGTACAAAGAATTGAGTTTTGCAATGGTCATGTCCACTTTCCCTTATGACTTCCCTGGATTCACTTTTCACCAAGCATTCAAGGCAAGAAAGAAGATAGAGAATATCATTCAAGGTGTGGTTGAGGAGAAAAGATCAAGAAGATTTGAAAATGATAAAACAAATGAAGTGCAATGTCAAGTGGACAAAATGGTTGTAGCCATTGATGAGAATGGGGAAAAATTATATAATAACAATCTCATCAGAGATTTGCTTCTTCGGGTATTATTCGCTGGTCATAGTACTCCTGCCATTGCTGCTTTTTGGGCTCTGCTACATATCTCACAAAACCCTCATGTTTTCCAGAAGGCCAAGGAAGAGCAAGAGTCGATCATAAGACAAAGACCATCTAATAAAAAAGGCTTAACTCTTAACGAAATCAAACAAATGAAATATCTCACAAAGGTCATTAACGAAGTTTTGAGAAGAAATACAATTACTCCCACAAATTTTCGTGAGGCAAAAACAGATGTAAACATCAATGGTTATTTTATTCCAAAAGGATGGACGATACAAATTTGGAACATAGCTGTTCATATGGACCCTCAAATTTATTCTAATCCACAAGAATTTAATCCTTCAAGATGGGAAAATTATACACCAAAGCCAGGAACCTTCATCCCTTTTGGACTTGGAAGTAGGTTTTGTCCTGGAAGTGAACTTGCAAGGCTTGAGATTACTATTCTACTTCATCATTTCATCCTCAATTACAAGTAT

Coding sequence (CDS)

ATGCAGGTTTGGAGCTTCACAAGCTATTCGAGGATGGAAATGATAAGTGTTTGTGTAATTATTGGAGTTGTCTTAGGAGTTTCTTTGTTGGTGTTTAGGCGACTCAATGAATTATGGTATTCGGTTAAGTTAGGAGGAAGAGCTTACAAGTCTCTTCCTCCTGGTGACTTGGGATGGCCTGTTATTGGCTCTTCCTTCTCCTCTTACAAAACATTCATGGTTCAAGAAGATCCAATCTCTTTTATTCAATCTCTTCACTCAAGATATGGAGAAGGGGGAATATATAAAACTCACCTTTATGGAAAGCCAACAGTGATTGCCACAGATCCAGAAATATGTCGACGGATATACTTAGACGAAGCAAAGTTCAAACAACATAATCCAGAGTCGGTGAAAATCCTAGAAGGATCAAGTGGTGATTTCTCAAAGATGGATCACAAAATTGCATATAAAGTAGTGGCAACACCCATGAATGGGTCTGAGGTATTATCTAACTATGTGGATTTCATTGAGGAAGTAATAGCGAAAGGGTTGGAGGAATGGAGTAGCATGTTGAGAGAGCCTATTAAATTGTTGGATGAAATTGGAAGTCTCTTTTTCAAAGTCATTACAAAAGTTTTCTTAGGGTCTCGACTTGATGCCAAAACCATGGTAGAGCTTCATGCATTGTACAAAGAATTGAGTTTTGCAATGGTCATGTCCACTTTCCCTTATGACTTCCCTGGATTCACTTTTCACCAAGCATTCAAGGCAAGAAAGAAGATAGAGAATATCATTCAAGGTGTGGTTGAGGAGAAAAGATCAAGAAGATTTGAAAATGATAAAACAAATGAAGTGCAATGTCAAGTGGACAAAATGGTTGTAGCCATTGATGAGAATGGGGAAAAATTATATAATAACAATCTCATCAGAGATTTGCTTCTTCGGGTATTATTCGCTGGTCATAGTACTCCTGCCATTGCTGCTTTTTGGGCTCTGCTACATATCTCACAAAACCCTCATGTTTTCCAGAAGGCCAAGGAAGAGCAAGAGTCGATCATAAGACAAAGACCATCTAATAAAAAAGGCTTAACTCTTAACGAAATCAAACAAATGAAATATCTCACAAAGGTCATTAACGAAGTTTTGAGAAGAAATACAATTACTCCCACAAATTTTCGTGAGGCAAAAACAGATGTAAACATCAATGGTTATTTTATTCCAAAAGGATGGACGATACAAATTTGGAACATAGCTGTTCATATGGACCCTCAAATTTATTCTAATCCACAAGAATTTAATCCTTCAAGATGGGAAAATTATACACCAAAGCCAGGAACCTTCATCCCTTTTGGACTTGGAAGTAGGTTTTGTCCTGGAAGTGAACTTGCAAGGCTTGAGATTACTATTCTACTTCATCATTTCATCCTCAATTACAAGTAT

Protein sequence

MQVWSFTSYSRMEMISVCVIIGVVLGVSLLVFRRLNELWYSVKLGGRAYKSLPPGDLGWPVIGSSFSSYKTFMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHNPESVKILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKLLDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQAFKARKKIENIIQGVVEEKRSRRFENDKTNEVQCQVDKMVVAIDENGEKLYNNNLIRDLLLRVLFAGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNKKGLTLNEIKQMKYLTKVINEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIYSNPQEFNPSRWENYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILNYKY
Homology
BLAST of MELO3C003385.jh1 vs. NCBI nr
Match: XP_008466645.1 (PREDICTED: beta-amyrin 11-oxidase-like, partial [Cucumis melo])

HSP 1 Score: 892 bits (2306), Expect = 0.0
Identity = 448/460 (97.39%), Postives = 452/460 (98.26%), Query Frame = 0

Query: 12  MEMISVCVIIGVVLGVSLLVFRRLNELWYSVKLGGRAYKSLPPGDLGWPVIGSSFSSYKT 71
           MEMISVCV IGVVLGVSLLVFRRLNELWY VKLGG+AYKSLPPGDLGWPVIGSSFSSYKT
Sbjct: 1   MEMISVCVTIGVVLGVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKT 60

Query: 72  FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHNPESV 131
           FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKP VIATD EICRRIYLDEAKFKQHNPESV
Sbjct: 61  FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESV 120

Query: 132 KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 191
           KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL
Sbjct: 121 KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 180

Query: 192 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQAFKA 251
           LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQA KA
Sbjct: 181 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKA 240

Query: 252 RKKIENIIQGVVEEKRSRRFENDKTNEVQCQVDKMVVAIDENGEKLYNNNLIRDLLLRVL 311
           RKKIENIIQGVVEEKR RRFENDKT+EVQCQVDKMVVAIDENG KLYNNNLIRDLLL VL
Sbjct: 241 RKKIENIIQGVVEEKR-RRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVL 300

Query: 312 FAGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNKKGLTLNEIKQMKYLTKV 371
           FAGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSN+KGLTLNEIKQMKYLTKV
Sbjct: 301 FAGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTKV 360

Query: 372 INEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIYSNPQEFNPSRW 431
           INEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQI+SNPQEFNPSRW
Sbjct: 361 INEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSRW 420

Query: 432 ENYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILN 471
           ENYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILN
Sbjct: 421 ENYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILN 459

BLAST of MELO3C003385.jh1 vs. NCBI nr
Match: XP_004148515.2 (beta-amyrin 11-oxidase [Cucumis sativus] >AIT72036.1 cytochrome P450 [Cucumis sativus] >KGN60415.1 hypothetical protein Csa_001840 [Cucumis sativus])

HSP 1 Score: 829 bits (2141), Expect = 8.50e-301
Identity = 411/462 (88.96%), Postives = 435/462 (94.16%), Query Frame = 0

Query: 12  MEMISVCVIIGVVLGVSLLVFRRLNELWYSVKLGGRAYKSLPPGDLGWPVIGSSFSSYKT 71
           MEMISVCVIIGVVLGV LLV RRLNELWY VKLGGRAYKSLPPGDLGWPVIG SFSSYKT
Sbjct: 1   MEMISVCVIIGVVLGVCLLV-RRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKT 60

Query: 72  FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHNPESV 131
           F+VQEDPISFIQSLHSRYG+GG+YKTHLYG PTVI T+PEICRRIYLDEA FKQH P+SV
Sbjct: 61  FIVQEDPISFIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSV 120

Query: 132 KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 191
           KILEGSSGDFS MDHKIAYKV+A+PMNG EVLSNYVDFIEEVIAKGLEEWSSM+REPIKL
Sbjct: 121 KILEGSSGDFSNMDHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIKL 180

Query: 192 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQAFKA 251
           +DEIG LFFKVITK+FLGS+LDAKTMVELH LYKELSF MVMSTFPYDFPGFTFHQ  KA
Sbjct: 181 VDEIGILFFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKA 240

Query: 252 RKKIENIIQGVVEEKRSRRFENDKTNEVQCQVDKMVVAIDENGEKLYNNNLIRDLLLRVL 311
           R+KIEN IQGVVEEK  RRFENDKT+EVQCQVDK+VVAI+ENG KLY+NN IRDL+L + 
Sbjct: 241 RRKIENTIQGVVEEK-IRRFENDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLILGIF 300

Query: 312 FAGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNKKGLTLNEIKQMKYLTKV 371
           FAGHSTPAIAA WALLHISQNPHVFQKAKEEQESIIRQRPS +KGLTLNEIKQMKYLTK+
Sbjct: 301 FAGHSTPAIAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTKI 360

Query: 372 INEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIYSNPQEFNPSRW 431
           INEVLRRNTIT TNFRE KTDVNING+FIPKGWT+QIWNIAVHMDPQIYSNPQEFNPSRW
Sbjct: 361 INEVLRRNTITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSRW 420

Query: 432 ENYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILNYK 473
           +NYTPKPG FIPFGLGSRFCPGSELA+LEITILLHHFILNYK
Sbjct: 421 DNYTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYK 460

BLAST of MELO3C003385.jh1 vs. NCBI nr
Match: XP_011652660.1 (beta-amyrin 11-oxidase [Cucumis sativus])

HSP 1 Score: 658 bits (1698), Expect = 2.33e-233
Identity = 320/463 (69.11%), Postives = 385/463 (83.15%), Query Frame = 0

Query: 12  MEMISVCVIIGVVLGVSLLVFRRLNELWYSVKLGGRAYKSLPPGDLGWPVIGSSFSSYKT 71
           ME+++VCVIIGVVLG  + V RR NE+WY  KLG +  KSLPPGDLGWP +GS+FS YK 
Sbjct: 1   MELMNVCVIIGVVLGFYVFV-RRFNEIWYLFKLGRKLNKSLPPGDLGWPFVGSTFSFYKA 60

Query: 72  FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHNPESV 131
           F V+ DP +FI +L  RYG  G+YK+HLYG PT+I T+PEICRRIYLD+ +F+ + P+SV
Sbjct: 61  FKVEGDPYTFIHTLLLRYGRVGMYKSHLYGMPTIIVTNPEICRRIYLDDERFEPNYPKSV 120

Query: 132 KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 191
           KILE ++GDFSK+DHK  YK++A+PMNGSEVLS +V+FIE+ + KGLEEWSSM REPI+L
Sbjct: 121 KILE-TNGDFSKIDHKSGYKIMASPMNGSEVLSKHVEFIEQTVEKGLEEWSSMRREPIEL 180

Query: 192 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQAFKA 251
           +DEIG L FK+I  +FLG+ +D + M ELH LYKEL   ++MS  PYD PGFT+ +A KA
Sbjct: 181 VDEIGGLLFKIILHIFLGNEIDGQAMAELHTLYKELGL-VIMSFLPYDLPGFTYRRALKA 240

Query: 252 RKKIENIIQGVVEEKRSRRFENDKTNEV-QCQVDKMVVAIDENGEKLYNNNLIRDLLLRV 311
           RKKIE I+  V+E+KR R  ++D TNEV  CQVDK++VA +ENG K YNN+ I DL+L +
Sbjct: 241 RKKIEKILHCVIEKKRKRFEKDDGTNEVVHCQVDKLIVATNENGSKPYNNSTIIDLILGI 300

Query: 312 LFAGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNKKGLTLNEIKQMKYLTK 371
            FAGH+TPAIAA WALLHISQNPH+FQ AKEEQESIIRQRPS +KGLT  EIKQMKYLTK
Sbjct: 301 FFAGHNTPAIAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKYLTK 360

Query: 372 VINEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIYSNPQEFNPSR 431
            INEVLRRNT+ PTNFR+A+T VNINGY IPKGWT+QIW++A+HMD QIYSN QEF+PSR
Sbjct: 361 FINEVLRRNTVAPTNFRKARTYVNINGYTIPKGWTVQIWSVAIHMDSQIYSNSQEFDPSR 420

Query: 432 WENYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILNYK 473
           W+NYTPKPG FIPFGLGSRFCPGSELA+LEITILLHHFILNYK
Sbjct: 421 WDNYTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYK 460

BLAST of MELO3C003385.jh1 vs. NCBI nr
Match: KAA0038545.1 (beta-amyrin 11-oxidase-like [Cucumis melo var. makuwa] >TYK31141.1 beta-amyrin 11-oxidase-like [Cucumis melo var. makuwa])

HSP 1 Score: 647 bits (1670), Expect = 1.75e-231
Identity = 330/340 (97.06%), Postives = 332/340 (97.65%), Query Frame = 0

Query: 1   MQVWSFTSYSRMEMISVCVIIGVVLGVSLLVFRRLNELWYSVKLGGRAYKSLPPGDLGWP 60
           MQVWSFTSYSRMEMISVCV IGVVLGVSLLVFRRLNELWY VKLGG+AYKSLPPGDLGWP
Sbjct: 1   MQVWSFTSYSRMEMISVCVTIGVVLGVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWP 60

Query: 61  VIGSSFSSYKTFMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPTVIATDPEICRRIYLDE 120
           VIGSSFSSYKTFMVQEDPISFIQSLHSRYGEGGIYKTHLYGKP VIATD EICRRIYLDE
Sbjct: 61  VIGSSFSSYKTFMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDE 120

Query: 121 AKFKQHNPESVKILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEE 180
           AKFKQHNPESVKILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEE
Sbjct: 121 AKFKQHNPESVKILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEE 180

Query: 181 WSSMLREPIKLLDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDF 240
           WSSMLREPIKLLDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDF
Sbjct: 181 WSSMLREPIKLLDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDF 240

Query: 241 PGFTFHQAFKARKKIENIIQGVVEEKRSRRFENDKTNEVQCQVDKMVVAIDENGEKLYNN 300
           PGFTFHQA KARKKIENIIQGVVEEKR RRFENDKT+EVQCQVDKMVVAIDENG KLYNN
Sbjct: 241 PGFTFHQALKARKKIENIIQGVVEEKR-RRFENDKTSEVQCQVDKMVVAIDENGAKLYNN 300

Query: 301 NLIRDLLLRVLFAGHSTPAIAAFWALLHISQNPHVFQKAK 340
           NLIRDLLL VLFAGHSTPAIAAFWALLHISQNPHVFQKAK
Sbjct: 301 NLIRDLLLGVLFAGHSTPAIAAFWALLHISQNPHVFQKAK 339

BLAST of MELO3C003385.jh1 vs. NCBI nr
Match: KAG6571834.1 (Beta-amyrin 11-oxidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 629 bits (1623), Expect = 1.78e-222
Identity = 311/462 (67.32%), Postives = 379/462 (82.03%), Query Frame = 0

Query: 12  MEMISVCVIIGVVLGVSLLVFRRLNELWYSVKLGGRAYKSLPPGDLGWPVIGSSFSSYKT 71
           MEMI+VC IIG VLGV + V R+LNE+WY VKLG +AYKSLPPGD+GWP++GSSFS YK 
Sbjct: 1   MEMINVCTIIGAVLGVYMFV-RKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKA 60

Query: 72  FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHNPESV 131
           F V  DP SFI  L SR G  G+YK+HLYG+PT+I TDPEICRRIYLD+  FK + P+SV
Sbjct: 61  FTVGGDPDSFIYHLLSRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSV 120

Query: 132 KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 191
           KILE  +G F++ DHK AY+++A PMNG EVLS+YVDFI++V+AKGLEEWSSM +EPI+L
Sbjct: 121 KILE-VNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSM-KEPIEL 180

Query: 192 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQAFKA 251
           L EIGSLFFKVI+++FLG+ + A  M EL  LYK+L  A ++S  PYD PG  +H+A KA
Sbjct: 181 LGEIGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPA-ILSILPYDVPGLVYHRALKA 240

Query: 252 RKKIENIIQGVVEEKRSRRFENDKTNEVQCQVDKMVVAIDENGEKLYNNNLIRDLLLRVL 311
           RKKIEN+++ V+EEKR R  E  +T EV  Q+DK+++A DENG KLY+NN I DLLL +L
Sbjct: 241 RKKIENVLECVIEEKR-RALEKKETREVHSQMDKLIMARDENGMKLYDNNTIIDLLLGLL 300

Query: 312 FAGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNKKGLTLNEIKQMKYLTKV 371
            AGH TPA AA WAL+HIS+ PH+F KAKEEQESI++QRPS +KGLT  EIKQMKYL K 
Sbjct: 301 HAGHHTPAYAAMWALVHISEKPHIFHKAKEEQESILKQRPSTQKGLTFQEIKQMKYLMKF 360

Query: 372 INEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIYSNPQEFNPSRW 431
           INE+LRRNTI   +FR+AKTD+NIN Y IPKGW++ IW  AVHMDPQ+YSNP+EF+PSRW
Sbjct: 361 INELLRRNTIAVASFRKAKTDININDYIIPKGWSVLIWTRAVHMDPQLYSNPEEFDPSRW 420

Query: 432 ENYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILNYK 473
           EN++PKPGTFIPFG+G+RFCPGSEL +LEITILLHHFILNYK
Sbjct: 421 ENHSPKPGTFIPFGIGTRFCPGSELTKLEITILLHHFILNYK 457

BLAST of MELO3C003385.jh1 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 2.4e-105
Identity = 208/469 (44.35%), Postives = 301/469 (64.18%), Query Frame = 0

Query: 12  MEMISVCVIIGVVLGVSLL---VFRRLNELWYSVKLGGRAYKSLPPGDLGWPVIGSSFSS 71
           ME+  VC+    +L   +      R LN  +Y VKL  R    LPPGD+GWP+IG   S 
Sbjct: 1   MEVHWVCMSAATLLVCYIFGSKFVRNLNGWYYDVKL-RRKEHPLPPGDMGWPLIGDLLSF 60

Query: 72  YKTFMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHNP 131
            K F     P SFI +L  +YG  GIYKTHL+G P++I  +P++CRR+  D+  FK   P
Sbjct: 61  IKDFS-SGHPDSFINNLVLKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYP 120

Query: 132 ESVKILEGSSG--DFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLR 191
           +S+K L       D S  +H++  +++ +P+ G + L+ Y++ +EE++   LEE SSM +
Sbjct: 121 KSIKELARCRPMIDVSNAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSM-K 180

Query: 192 EPIKLLDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFH 251
            P++LL E+  + FK I  VF+GS  +   + ++ + + +L   M   + P + PGFTFH
Sbjct: 181 HPVELLKEMKKVSFKAIVHVFMGSS-NQDIIKKIGSSFTDLYNGMF--SIPINVPGFTFH 240

Query: 252 QAFKARKKIENIIQGVVEEKRSRRFENDKTNEVQCQ--VDKMVVAIDENGEKLYNNNLIR 311
           +A +ARKK+  I+Q VV+E+R    EN      Q +  +D ++   DENG KL + + I 
Sbjct: 241 KALEARKKLAKIVQPVVDERR-LMIENGPQEGSQRKDLIDILLEVKDENGRKLEDED-IS 300

Query: 312 DLLLRVLFAGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNKKGLTLNEIKQ 371
           DLL+ +LFAGH + A +  W++ +++Q+PH+ +KAKEEQE I R R S++K L+L EIKQ
Sbjct: 301 DLLIGLLFAGHESTATSLMWSITYLTQHPHILKKAKEEQEEITRTRFSSQKQLSLKEIKQ 360

Query: 372 MKYLTKVINEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIYSNPQ 431
           M YL++VI+E LR   I    FREA  DVNINGY IPKGW + IW  A+HMD + Y NP+
Sbjct: 361 MVYLSQVIDETLRCANIAFATFREATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPE 420

Query: 432 EFNPSRWENYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILNYK 474
           EFNPSRW++Y  K GTF+PFG GSR CPG++LA+LEI+I LH+F+ NY+
Sbjct: 421 EFNPSRWDDYNAKAGTFLPFGAGSRLCPGADLAKLEISIFLHYFLRNYR 461

BLAST of MELO3C003385.jh1 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 366.7 bits (940), Expect = 4.0e-100
Identity = 203/455 (44.62%), Postives = 285/455 (62.64%), Query Frame = 0

Query: 23  VVLGVSLL--VFRRLNELWYSVKLGGRAYKSLPPGDLGWPVIGSSFSSYKTFMVQEDPIS 82
           ++LG+ +L  V +R+N   Y  KLG + +  LPPGDLGWPVIG+ +S  + F    DP S
Sbjct: 14  IILGLFVLKWVLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKT-SDPES 73

Query: 83  FIQSLHSRYGEGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHNPESVKILEG--SS 142
           FIQS  +RYG  GIYK H++G P V+ T PE CRR+  D+  F    P+S   L G  S 
Sbjct: 74  FIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSF 133

Query: 143 GDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKLLDEIGSL 202
              S  +HK   ++ + P+NG E LS Y+ FIEE +   LE+WS M    I+ L  +  L
Sbjct: 134 VGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM--GEIEFLSHLRKL 193

Query: 203 FFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQAFKARKKIENI 262
            FKVI  +FL S  +   M  L   Y  L++   +     + PGF +H+A KARKK+   
Sbjct: 194 TFKVIMYIFLSSESE-HVMDSLEREYTNLNYG--VRAMGINLPGFAYHRALKARKKLVAA 253

Query: 263 IQGVVEEKRSRRFENDKTNEVQCQVDKMVVAIDENGEKLYNNNLIRDLLLRVLFAGHSTP 322
            Q +V  +R++R +N  +N  +  +D ++   DENG  L +  +I DLLL  L AGH + 
Sbjct: 254 FQSIVTNRRNQRKQNISSNR-KDMLDNLIDVKDENGRVLDDEEII-DLLLMYLNAGHESS 313

Query: 323 AIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNKKGLTLNEIKQMKYLTKVINEVLRR 382
                WA + + ++P + QKAKEEQE I+++R   +K LTL E ++M YL++VI+E LR 
Sbjct: 314 GHLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRV 373

Query: 383 NTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIYSNPQEFNPSRWENYTPKP 442
            T + T FREAK+DV ++GY IPKGW +  W   VH+DP+IY +P++F+PSRWE YTPK 
Sbjct: 374 ITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKA 433

Query: 443 GTFIPFGLGSRFCPGSELARLEITILLHHFILNYK 474
           GTF+PFGLGS  CPG++LA+LEI+I LHHF+L Y+
Sbjct: 434 GTFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYR 458

BLAST of MELO3C003385.jh1 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 4.5e-96
Identity = 174/462 (37.66%), Postives = 287/462 (62.12%), Query Frame = 0

Query: 14  MISVCVIIGVVLGVSLLVFRRLNELWYSVKLGGRAYKSLPPGDLGWPVIGSSFSSYKTFM 73
           +++  V+ GVVL  +  V RR ++      LG      LPPG++GWP++GS ++  + F 
Sbjct: 19  VLAAAVVAGVVLVDA--VVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAFK 78

Query: 74  VQEDPISFIQSLHSRYGEGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHNPESVKI 133
              +P +FI S   R+G  G+Y+T ++  PT++A  PE C+++ +D+  F    P++   
Sbjct: 79  -SGNPDAFIASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVT 138

Query: 134 LEG--SSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 193
           L G  S  + S  DH+   K+ A P+NG + L+ Y+ FI++ +   L  WSS     ++ 
Sbjct: 139 LIGPKSFVNMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEF 198

Query: 194 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQAFKA 253
           L E+  + FK+I ++F+    DA TM  L   Y +L++   M     + PGF +++A +A
Sbjct: 199 LTELRRMTFKIIVQIFMSGADDA-TMEALERSYTDLNYG--MRAMAINLPGFAYYRALRA 258

Query: 254 RKKIENIIQGVVEEKRSRRFENDKTNEVQCQVDKMVVAIDENGEKLYNNNLIRDLLLRVL 313
           R+K+ +++QGV++ +R+   +  K +     +D+++ A DE G +L ++ ++ D+L+  L
Sbjct: 259 RRKLVSVLQGVLDGRRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDEIV-DVLIMYL 318

Query: 314 FAGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNKKGLTLNEIKQMKYLTKV 373
            AGH +      WA + + +NP +F +AK EQE I+R  P+ + GLTL + K+M +L++V
Sbjct: 319 NAGHESSGHITMWATVFLQENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQV 378

Query: 374 INEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIYSNPQEFNPSRW 433
           ++E LR   I+  +FR+A  D+ +NGY IPKGW +Q+W  +VHMD Q+Y +P+ FNPSRW
Sbjct: 379 VDETLRCVNISFVSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRW 438

Query: 434 ENYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILNYK 474
           E   PK GTF+PFGLG+R CPG++LA+LEI++ LHHF+L YK
Sbjct: 439 EGPPPKAGTFLPFGLGARLCPGNDLAKLEISVFLHHFLLGYK 473

BLAST of MELO3C003385.jh1 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 1.0e-92
Identity = 172/463 (37.15%), Postives = 280/463 (60.48%), Query Frame = 0

Query: 16  SVCVIIGVVLGVSLLVFRRLNELWY-SVKLGGRAYKSLPPGDLGWPVIGSSFSSYKTFMV 75
           +V  ++  +  V+L    R    WY +  LG      LPPGD+GWP++G  ++  + F  
Sbjct: 8   AVAAVVAALAVVALDAAVRAAHAWYWTASLGAGRRGRLPPGDMGWPLVGGMWAFLRAFK- 67

Query: 76  QEDPISFIQSLHSRYGEGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHNPESVKIL 135
              P SFI S   R+G  G+Y+  ++  PT++AT PE C+++ +D+  F    P++   L
Sbjct: 68  SGRPDSFIDSFARRFGRAGLYRAFMFSSPTIMATTPEACKQVLMDDDAFVTGWPKATVAL 127

Query: 136 EG--SSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLRE-PIKL 195
            G  S  +    +H+   K+ A P+NG + L++Y+ FI++ +   L  WS    +   + 
Sbjct: 128 IGPKSFVNMGYDEHRRLRKLTAAPINGFDALTSYLGFIDDTVVTTLRGWSERGGDGHFEF 187

Query: 196 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQAFKA 255
           L E+  + F++I ++F+G   D +T  EL   Y EL++   M     D PGF +H+A +A
Sbjct: 188 LTELRRMTFRIIVQIFMGG-ADERTAAELERTYTELNYG--MRAMAIDLPGFAYHKAIRA 247

Query: 256 RKKIENIIQGVVEEKRSRRFENDKTNEVQC-QVDKMVVAIDENGEKLYNNNLIRDLLLRV 315
           R+++   +Q V++E+R+R  +           +D+++   DE G +L ++ +I D+L+  
Sbjct: 248 RRRLVAALQRVLDERRARGGKTAAGAAAPVDMMDRLIAVEDEGGRRLQDDEII-DVLVMY 307

Query: 316 LFAGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNKKGLTLNEIKQMKYLTK 375
           L AGH +      WA + + +NP +  KAK EQE+I+R  P  +KGLTL + ++M YL++
Sbjct: 308 LNAGHESSGHITMWATVFLQENPEILAKAKAEQEAIMRSIPPGQKGLTLRDFRKMAYLSQ 367

Query: 376 VINEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIYSNPQEFNPSR 435
           V++E LR   I+  +FR+A  DV +NGY IPKGW +Q+W  +VHMDPQ+Y +P++F+PSR
Sbjct: 368 VVDETLRFVNISFVSFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPKKFDPSR 427

Query: 436 WENYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILNYK 474
           WE   P+ GTF+PFGLG+R CPG++LA+LEI++ LHHF+L YK
Sbjct: 428 WEGPPPRAGTFLPFGLGTRLCPGNDLAKLEISVFLHHFLLGYK 465

BLAST of MELO3C003385.jh1 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 1.8e-92
Identity = 183/451 (40.58%), Postives = 277/451 (61.42%), Query Frame = 0

Query: 26  GVSLLVFRRLNELWYSVKLGGRAYKSLPPGDLGWPVIGSSFSSYKTFMVQEDPISFIQSL 85
           G++ LV R++N   Y   LG   +  LPPGDLGWP IG+  S  + F    DP SF ++L
Sbjct: 21  GLNWLV-RKVNVWLYESSLGENRH-YLPPGDLGWPFIGNMLSFLRAFKT-SDPDSFTRTL 80

Query: 86  HSRYGEGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHNPESVKILEG--SSGDFSK 145
             RYG  GIYK H++G P++I T  + CRR+  D+  FK   P S   L G  S    S 
Sbjct: 81  IKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISF 140

Query: 146 MDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKLLDEIGSLFFKVI 205
            +HK   ++ A P+NG E LS Y+ +IEE +   L++W+ M     + L  +  L F++I
Sbjct: 141 EEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKM--GEFEFLTHLRKLTFRII 200

Query: 206 TKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQAFKARKKIENIIQGVV 265
             +FL S  +   M  L   Y  L++   +     + PGF +H+A KARK +    Q +V
Sbjct: 201 MYIFLSSESE-NVMDALEREYTALNYG--VRAMAVNIPGFAYHRALKARKTLVAAFQSIV 260

Query: 266 EEKRSRRFENDKTNEVQCQVDKMVVAIDENGEKLYNNNLIRDLLLRVLFAGHSTPAIAAF 325
            E+R++R +N  +N+ +  +D ++   DE+G+ L +  +I D+LL  L AGH +      
Sbjct: 261 TERRNQRKQNILSNK-KDMLDNLLNVKDEDGKTLDDEEII-DVLLMYLNAGHESSGHTIM 320

Query: 326 WALLHISQNPHVFQKAKEEQESIIRQRPSNKKGLTLNEIKQMKYLTKVINEVLRRNTITP 385
           WA + + ++P V Q+AK EQE I++ RP  +KGL+L E ++M++L++V++E LR  T + 
Sbjct: 321 WATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSL 380

Query: 386 TNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIYSNPQEFNPSRWEN-YTPKPGTFI 445
           T FREAKTDV +NGY IPKGW +  W   VH+DP+++ +P++F+P+RW+N + PK G F+
Sbjct: 381 TAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFL 440

Query: 446 PFGLGSRFCPGSELARLEITILLHHFILNYK 474
           PFG GS  CPG++LA+LEI+I LHHF+L Y+
Sbjct: 441 PFGAGSHLCPGNDLAKLEISIFLHHFLLKYQ 461

BLAST of MELO3C003385.jh1 vs. ExPASy TrEMBL
Match: A0A1S3CRX8 (beta-amyrin 11-oxidase-like OS=Cucumis melo OX=3656 GN=LOC103504002 PE=3 SV=1)

HSP 1 Score: 892 bits (2306), Expect = 0.0
Identity = 448/460 (97.39%), Postives = 452/460 (98.26%), Query Frame = 0

Query: 12  MEMISVCVIIGVVLGVSLLVFRRLNELWYSVKLGGRAYKSLPPGDLGWPVIGSSFSSYKT 71
           MEMISVCV IGVVLGVSLLVFRRLNELWY VKLGG+AYKSLPPGDLGWPVIGSSFSSYKT
Sbjct: 1   MEMISVCVTIGVVLGVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKT 60

Query: 72  FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHNPESV 131
           FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKP VIATD EICRRIYLDEAKFKQHNPESV
Sbjct: 61  FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESV 120

Query: 132 KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 191
           KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL
Sbjct: 121 KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 180

Query: 192 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQAFKA 251
           LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQA KA
Sbjct: 181 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKA 240

Query: 252 RKKIENIIQGVVEEKRSRRFENDKTNEVQCQVDKMVVAIDENGEKLYNNNLIRDLLLRVL 311
           RKKIENIIQGVVEEKR RRFENDKT+EVQCQVDKMVVAIDENG KLYNNNLIRDLLL VL
Sbjct: 241 RKKIENIIQGVVEEKR-RRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVL 300

Query: 312 FAGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNKKGLTLNEIKQMKYLTKV 371
           FAGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSN+KGLTLNEIKQMKYLTKV
Sbjct: 301 FAGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTKV 360

Query: 372 INEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIYSNPQEFNPSRW 431
           INEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQI+SNPQEFNPSRW
Sbjct: 361 INEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSRW 420

Query: 432 ENYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILN 471
           ENYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILN
Sbjct: 421 ENYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILN 459

BLAST of MELO3C003385.jh1 vs. ExPASy TrEMBL
Match: A0A097IYP6 (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa3G903550 PE=2 SV=1)

HSP 1 Score: 829 bits (2141), Expect = 4.11e-301
Identity = 411/462 (88.96%), Postives = 435/462 (94.16%), Query Frame = 0

Query: 12  MEMISVCVIIGVVLGVSLLVFRRLNELWYSVKLGGRAYKSLPPGDLGWPVIGSSFSSYKT 71
           MEMISVCVIIGVVLGV LLV RRLNELWY VKLGGRAYKSLPPGDLGWPVIG SFSSYKT
Sbjct: 1   MEMISVCVIIGVVLGVCLLV-RRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKT 60

Query: 72  FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHNPESV 131
           F+VQEDPISFIQSLHSRYG+GG+YKTHLYG PTVI T+PEICRRIYLDEA FKQH P+SV
Sbjct: 61  FIVQEDPISFIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSV 120

Query: 132 KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 191
           KILEGSSGDFS MDHKIAYKV+A+PMNG EVLSNYVDFIEEVIAKGLEEWSSM+REPIKL
Sbjct: 121 KILEGSSGDFSNMDHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIKL 180

Query: 192 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQAFKA 251
           +DEIG LFFKVITK+FLGS+LDAKTMVELH LYKELSF MVMSTFPYDFPGFTFHQ  KA
Sbjct: 181 VDEIGILFFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKA 240

Query: 252 RKKIENIIQGVVEEKRSRRFENDKTNEVQCQVDKMVVAIDENGEKLYNNNLIRDLLLRVL 311
           R+KIEN IQGVVEEK  RRFENDKT+EVQCQVDK+VVAI+ENG KLY+NN IRDL+L + 
Sbjct: 241 RRKIENTIQGVVEEK-IRRFENDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLILGIF 300

Query: 312 FAGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNKKGLTLNEIKQMKYLTKV 371
           FAGHSTPAIAA WALLHISQNPHVFQKAKEEQESIIRQRPS +KGLTLNEIKQMKYLTK+
Sbjct: 301 FAGHSTPAIAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTKI 360

Query: 372 INEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIYSNPQEFNPSRW 431
           INEVLRRNTIT TNFRE KTDVNING+FIPKGWT+QIWNIAVHMDPQIYSNPQEFNPSRW
Sbjct: 361 INEVLRRNTITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSRW 420

Query: 432 ENYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILNYK 473
           +NYTPKPG FIPFGLGSRFCPGSELA+LEITILLHHFILNYK
Sbjct: 421 DNYTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYK 460

BLAST of MELO3C003385.jh1 vs. ExPASy TrEMBL
Match: A0A5D3E6B2 (Beta-amyrin 11-oxidase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G004210 PE=4 SV=1)

HSP 1 Score: 647 bits (1670), Expect = 8.47e-232
Identity = 330/340 (97.06%), Postives = 332/340 (97.65%), Query Frame = 0

Query: 1   MQVWSFTSYSRMEMISVCVIIGVVLGVSLLVFRRLNELWYSVKLGGRAYKSLPPGDLGWP 60
           MQVWSFTSYSRMEMISVCV IGVVLGVSLLVFRRLNELWY VKLGG+AYKSLPPGDLGWP
Sbjct: 1   MQVWSFTSYSRMEMISVCVTIGVVLGVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWP 60

Query: 61  VIGSSFSSYKTFMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPTVIATDPEICRRIYLDE 120
           VIGSSFSSYKTFMVQEDPISFIQSLHSRYGEGGIYKTHLYGKP VIATD EICRRIYLDE
Sbjct: 61  VIGSSFSSYKTFMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDE 120

Query: 121 AKFKQHNPESVKILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEE 180
           AKFKQHNPESVKILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEE
Sbjct: 121 AKFKQHNPESVKILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEE 180

Query: 181 WSSMLREPIKLLDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDF 240
           WSSMLREPIKLLDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDF
Sbjct: 181 WSSMLREPIKLLDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDF 240

Query: 241 PGFTFHQAFKARKKIENIIQGVVEEKRSRRFENDKTNEVQCQVDKMVVAIDENGEKLYNN 300
           PGFTFHQA KARKKIENIIQGVVEEKR RRFENDKT+EVQCQVDKMVVAIDENG KLYNN
Sbjct: 241 PGFTFHQALKARKKIENIIQGVVEEKR-RRFENDKTSEVQCQVDKMVVAIDENGAKLYNN 300

Query: 301 NLIRDLLLRVLFAGHSTPAIAAFWALLHISQNPHVFQKAK 340
           NLIRDLLL VLFAGHSTPAIAAFWALLHISQNPHVFQKAK
Sbjct: 301 NLIRDLLLGVLFAGHSTPAIAAFWALLHISQNPHVFQKAK 339

BLAST of MELO3C003385.jh1 vs. ExPASy TrEMBL
Match: A0A6J1GJM1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 SV=1)

HSP 1 Score: 627 bits (1618), Expect = 1.47e-221
Identity = 310/462 (67.10%), Postives = 378/462 (81.82%), Query Frame = 0

Query: 12  MEMISVCVIIGVVLGVSLLVFRRLNELWYSVKLGGRAYKSLPPGDLGWPVIGSSFSSYKT 71
           MEMI+VC IIG VLGV + V R+LNE+WY VKLG +AYKSLPPGD+GWP++GSSFS Y  
Sbjct: 1   MEMINVCTIIGAVLGVYMFV-RKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNA 60

Query: 72  FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHNPESV 131
           F V  DP SFI  L SR G  G+YK+HLYG+PT+I TDPEICRRIYLD+  FK + P+SV
Sbjct: 61  FTVGGDPDSFIYHLLSRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSV 120

Query: 132 KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 191
           KILE  +G F++ DHK AY+++A PMNG EVLS+YVDFI++V+AKGLEEWSSM +EPI+L
Sbjct: 121 KILE-VNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSM-KEPIEL 180

Query: 192 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQAFKA 251
           L EIGSLFFKVI+++FLG+ + A  M EL  LYK+L  A ++S  PYD PG  +H+A KA
Sbjct: 181 LGEIGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPA-ILSILPYDVPGLVYHRALKA 240

Query: 252 RKKIENIIQGVVEEKRSRRFENDKTNEVQCQVDKMVVAIDENGEKLYNNNLIRDLLLRVL 311
           RKKIEN+++ V+EEKR R  E  +T EV  Q+DK+++A DENG KLY+NN I DLLL +L
Sbjct: 241 RKKIENVLECVIEEKR-RALEQKETREVHSQMDKLIMARDENGMKLYDNNTIIDLLLGLL 300

Query: 312 FAGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNKKGLTLNEIKQMKYLTKV 371
            AGH TPA AA WAL+HIS+ PH+F KAKEEQESI++QRPS +KGLT  EIKQMKYL K 
Sbjct: 301 HAGHHTPAYAAMWALVHISEKPHIFHKAKEEQESILKQRPSTQKGLTFQEIKQMKYLMKF 360

Query: 372 INEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIYSNPQEFNPSRW 431
           INE+LRRNTI   +FR+AKTD+NIN Y IPKGW++ IW  AVHMDPQ+YSNP+EF+PSRW
Sbjct: 361 INELLRRNTIVVASFRKAKTDININDYIIPKGWSVLIWTRAVHMDPQLYSNPEEFDPSRW 420

Query: 432 ENYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILNYK 473
           EN++PKPGTFIPFG+G+RFCPGSEL +LEITILLHHFILNYK
Sbjct: 421 ENHSPKPGTFIPFGIGTRFCPGSELTKLEITILLHHFILNYK 457

BLAST of MELO3C003385.jh1 vs. ExPASy TrEMBL
Match: A0A6J1GLR1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455409 PE=3 SV=1)

HSP 1 Score: 625 bits (1611), Expect = 1.64e-220
Identity = 314/462 (67.97%), Postives = 376/462 (81.39%), Query Frame = 0

Query: 12  MEMISVCVIIGVVLGVSLLVFRRLNELWYSVKLGGRAYKSLPPGDLGWPVIGSSFSSYKT 71
           ME I+VCVIIG VLGV + V R+LNE+WY VKLG +AYKSLPPGD+GWP++GSSFS Y  
Sbjct: 1   METINVCVIIGFVLGVYMFV-RKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNA 60

Query: 72  FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHNPESV 131
           F V  DP SFI  L SR G  G+YK+H YG+PT+I TDPEICRRIYLD+  FK + P+SV
Sbjct: 61  FTVGGDPDSFIYHLLSRNGRVGMYKSHFYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSV 120

Query: 132 KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 191
           KILE  +G F++ DHK AY+++A PMNG EVLS+YVDFI++V+AKGLEEWSSM +EPI+L
Sbjct: 121 KILE-VNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSM-KEPIEL 180

Query: 192 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQAFKA 251
           L EIGSLFFKVI+++FLG+ + A  M EL  LYK+L  A ++S  PYD PG TFH A KA
Sbjct: 181 LGEIGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPA-ILSILPYDLPGLTFHGALKA 240

Query: 252 RKKIENIIQGVVEEKRSRRFENDKTNEVQCQVDKMVVAIDENGEKLYNNNLIRDLLLRVL 311
           RKKIENI+Q V+EEKR R  E ++  EV+ Q+DK+++A DENG KLY+NN I DLLL +L
Sbjct: 241 RKKIENILQCVIEEKR-RALEKEE-REVESQMDKLIMARDENGMKLYDNNAIIDLLLGLL 300

Query: 312 FAGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNKKGLTLNEIKQMKYLTKV 371
            AGH TPA AA WAL+HIS+ PHVF KAKEEQESII+QRPS +KGLT  EIKQMKYL K 
Sbjct: 301 HAGHHTPAYAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMKF 360

Query: 372 INEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIYSNPQEFNPSRW 431
           INE+LRRNTI   +FR+AK DVNIN Y IPKGW + IW  A+HMDPQ+YSNPQEF+PSRW
Sbjct: 361 INELLRRNTIAVASFRKAKKDVNINDYIIPKGWNVLIWTRAIHMDPQLYSNPQEFDPSRW 420

Query: 432 ENYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILNYK 473
           ENY+PKPGTF+PFG+G+RFCPGSEL +LEITILLHHFILNYK
Sbjct: 421 ENYSPKPGTFLPFGIGTRFCPGSELTKLEITILLHHFILNYK 456

BLAST of MELO3C003385.jh1 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 366.7 bits (940), Expect = 2.8e-101
Identity = 203/455 (44.62%), Postives = 285/455 (62.64%), Query Frame = 0

Query: 23  VVLGVSLL--VFRRLNELWYSVKLGGRAYKSLPPGDLGWPVIGSSFSSYKTFMVQEDPIS 82
           ++LG+ +L  V +R+N   Y  KLG + +  LPPGDLGWPVIG+ +S  + F    DP S
Sbjct: 14  IILGLFVLKWVLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKT-SDPES 73

Query: 83  FIQSLHSRYGEGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHNPESVKILEG--SS 142
           FIQS  +RYG  GIYK H++G P V+ T PE CRR+  D+  F    P+S   L G  S 
Sbjct: 74  FIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSF 133

Query: 143 GDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKLLDEIGSL 202
              S  +HK   ++ + P+NG E LS Y+ FIEE +   LE+WS M    I+ L  +  L
Sbjct: 134 VGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM--GEIEFLSHLRKL 193

Query: 203 FFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQAFKARKKIENI 262
            FKVI  +FL S  +   M  L   Y  L++   +     + PGF +H+A KARKK+   
Sbjct: 194 TFKVIMYIFLSSESE-HVMDSLEREYTNLNYG--VRAMGINLPGFAYHRALKARKKLVAA 253

Query: 263 IQGVVEEKRSRRFENDKTNEVQCQVDKMVVAIDENGEKLYNNNLIRDLLLRVLFAGHSTP 322
            Q +V  +R++R +N  +N  +  +D ++   DENG  L +  +I DLLL  L AGH + 
Sbjct: 254 FQSIVTNRRNQRKQNISSNR-KDMLDNLIDVKDENGRVLDDEEII-DLLLMYLNAGHESS 313

Query: 323 AIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNKKGLTLNEIKQMKYLTKVINEVLRR 382
                WA + + ++P + QKAKEEQE I+++R   +K LTL E ++M YL++VI+E LR 
Sbjct: 314 GHLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRV 373

Query: 383 NTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIYSNPQEFNPSRWENYTPKP 442
            T + T FREAK+DV ++GY IPKGW +  W   VH+DP+IY +P++F+PSRWE YTPK 
Sbjct: 374 ITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKA 433

Query: 443 GTFIPFGLGSRFCPGSELARLEITILLHHFILNYK 474
           GTF+PFGLGS  CPG++LA+LEI+I LHHF+L Y+
Sbjct: 434 GTFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYR 458

BLAST of MELO3C003385.jh1 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 366.7 bits (940), Expect = 2.8e-101
Identity = 203/455 (44.62%), Postives = 285/455 (62.64%), Query Frame = 0

Query: 23  VVLGVSLL--VFRRLNELWYSVKLGGRAYKSLPPGDLGWPVIGSSFSSYKTFMVQEDPIS 82
           ++LG+ +L  V +R+N   Y  KLG + +  LPPGDLGWPVIG+ +S  + F    DP S
Sbjct: 14  IILGLFVLKWVLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKT-SDPES 73

Query: 83  FIQSLHSRYGEGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHNPESVKILEG--SS 142
           FIQS  +RYG  GIYK H++G P V+ T PE CRR+  D+  F    P+S   L G  S 
Sbjct: 74  FIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSF 133

Query: 143 GDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKLLDEIGSL 202
              S  +HK   ++ + P+NG E LS Y+ FIEE +   LE+WS M    I+ L  +  L
Sbjct: 134 VGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM--GEIEFLSHLRKL 193

Query: 203 FFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQAFKARKKIENI 262
            FKVI  +FL S  +   M  L   Y  L++   +     + PGF +H+A KARKK+   
Sbjct: 194 TFKVIMYIFLSSESE-HVMDSLEREYTNLNYG--VRAMGINLPGFAYHRALKARKKLVAA 253

Query: 263 IQGVVEEKRSRRFENDKTNEVQCQVDKMVVAIDENGEKLYNNNLIRDLLLRVLFAGHSTP 322
            Q +V  +R++R +N  +N  +  +D ++   DENG  L +  +I DLLL  L AGH + 
Sbjct: 254 FQSIVTNRRNQRKQNISSNR-KDMLDNLIDVKDENGRVLDDEEII-DLLLMYLNAGHESS 313

Query: 323 AIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNKKGLTLNEIKQMKYLTKVINEVLRR 382
                WA + + ++P + QKAKEEQE I+++R   +K LTL E ++M YL++VI+E LR 
Sbjct: 314 GHLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRV 373

Query: 383 NTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIYSNPQEFNPSRWENYTPKP 442
            T + T FREAK+DV ++GY IPKGW +  W   VH+DP+IY +P++F+PSRWE YTPK 
Sbjct: 374 ITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKA 433

Query: 443 GTFIPFGLGSRFCPGSELARLEITILLHHFILNYK 474
           GTF+PFGLGS  CPG++LA+LEI+I LHHF+L Y+
Sbjct: 434 GTFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYR 458

BLAST of MELO3C003385.jh1 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 341.3 bits (874), Expect = 1.3e-93
Identity = 183/451 (40.58%), Postives = 277/451 (61.42%), Query Frame = 0

Query: 26  GVSLLVFRRLNELWYSVKLGGRAYKSLPPGDLGWPVIGSSFSSYKTFMVQEDPISFIQSL 85
           G++ LV R++N   Y   LG   +  LPPGDLGWP IG+  S  + F    DP SF ++L
Sbjct: 21  GLNWLV-RKVNVWLYESSLGENRH-YLPPGDLGWPFIGNMLSFLRAFKT-SDPDSFTRTL 80

Query: 86  HSRYGEGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHNPESVKILEG--SSGDFSK 145
             RYG  GIYK H++G P++I T  + CRR+  D+  FK   P S   L G  S    S 
Sbjct: 81  IKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISF 140

Query: 146 MDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKLLDEIGSLFFKVI 205
            +HK   ++ A P+NG E LS Y+ +IEE +   L++W+ M     + L  +  L F++I
Sbjct: 141 EEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKM--GEFEFLTHLRKLTFRII 200

Query: 206 TKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQAFKARKKIENIIQGVV 265
             +FL S  +   M  L   Y  L++   +     + PGF +H+A KARK +    Q +V
Sbjct: 201 MYIFLSSESE-NVMDALEREYTALNYG--VRAMAVNIPGFAYHRALKARKTLVAAFQSIV 260

Query: 266 EEKRSRRFENDKTNEVQCQVDKMVVAIDENGEKLYNNNLIRDLLLRVLFAGHSTPAIAAF 325
            E+R++R +N  +N+ +  +D ++   DE+G+ L +  +I D+LL  L AGH +      
Sbjct: 261 TERRNQRKQNILSNK-KDMLDNLLNVKDEDGKTLDDEEII-DVLLMYLNAGHESSGHTIM 320

Query: 326 WALLHISQNPHVFQKAKEEQESIIRQRPSNKKGLTLNEIKQMKYLTKVINEVLRRNTITP 385
           WA + + ++P V Q+AK EQE I++ RP  +KGL+L E ++M++L++V++E LR  T + 
Sbjct: 321 WATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSL 380

Query: 386 TNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIYSNPQEFNPSRWEN-YTPKPGTFI 445
           T FREAKTDV +NGY IPKGW +  W   VH+DP+++ +P++F+P+RW+N + PK G F+
Sbjct: 381 TAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFL 440

Query: 446 PFGLGSRFCPGSELARLEITILLHHFILNYK 474
           PFG GS  CPG++LA+LEI+I LHHF+L Y+
Sbjct: 441 PFGAGSHLCPGNDLAKLEISIFLHHFLLKYQ 461

BLAST of MELO3C003385.jh1 vs. TAIR 10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )

HSP 1 Score: 211.1 bits (536), Expect = 2.0e-54
Identity = 138/433 (31.87%), Postives = 212/433 (48.96%), Query Frame = 0

Query: 52  LPPGDLGWPVIGSSFSSYKTFMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPTVIATDPE 111
           LPPG +GWP +G +   Y      ++P  F  S   RYGE  I+KT + G P V+   PE
Sbjct: 34  LPPGSMGWPYLGETLQLY-----SQNPNVFFTSKQKRYGE--IFKTRILGYPCVMLASPE 93

Query: 112 ICRRIYLDEA-KFKQHNPESVKILEGSSG-DFSKMDHKIAYKVVATPMNGSEVLSNYVDF 171
             R + +  A  FK   P S + L G S   F + D+    + +       E +   +  
Sbjct: 94  AARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGDYHSHIRKLVQSSFYPETIRKLIPD 153

Query: 172 IEEVIAKGLEEWSSMLREPIKLLDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKEL-- 231
           IE +    L+ W++M    +    E+    F V      G          L + YKE+  
Sbjct: 154 IEHIALSSLQSWANM--PIVSTYQEMKKFAFDVGILAIFG---------HLESSYKEILK 213

Query: 232 -SFAMV---MSTFPYDFPGFTFHQAFKARKKIENIIQGVVEEKRSRRFENDKTNEVQCQV 291
            ++ +V    ++FP   PG ++H+A  ARK+++ I+  ++ E+R +R           Q 
Sbjct: 214 HNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERREKR---------ALQT 273

Query: 292 DKMVVAIDENGEK--LYNNNLIRDLLLRVLFAGHSTPAIAAFWALLHISQNPHVFQKAKE 351
           D +   ++   EK  +     I D ++ VLFA   T A    W L ++  +  + +  K 
Sbjct: 274 DFLGHLLNFKNEKGRVLTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQKLLEAVKA 333

Query: 352 EQESIIRQRPSNKKGLTLNEIKQMKYLTKVINEVLRRNTITPTNFREAKTDVNINGYFIP 411
           EQ++I  +    KK LT  + + M    KVI E LR  +I    FREA  DV   GY IP
Sbjct: 334 EQKAIYEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVEYKGYLIP 393

Query: 412 KGWTIQIWNIAVHMDPQIYSNPQEFNPSRWENYTPKPGTFIPFGLGSRFCPGSELARLEI 471
           KGW +      +H +P+ +SNP+ F+PSR+E   PKP TF+PFG G   CPG+ELA+L+I
Sbjct: 394 KGWKVMPLFRNIHHNPKYFSNPEVFDPSRFE-VNPKPNTFMPFGSGVHACPGNELAKLQI 438

Query: 472 TILLHHFILNYKY 475
            I LHH + N+++
Sbjct: 454 LIFLHHLVSNFRW 438

BLAST of MELO3C003385.jh1 vs. TAIR 10
Match: AT1G12740.1 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 208.8 bits (530), Expect = 9.8e-54
Identity = 132/441 (29.93%), Postives = 224/441 (50.79%), Query Frame = 0

Query: 39  WYSVKLGGRAYKSLPPGDLGWPVIGSSFSSYKTFMVQEDPISFIQSLHSRYGEGGIYKTH 98
           W + K  G+    LPPG +G+P++G S   +K     + P  FI+    +YG   I+KT+
Sbjct: 22  WRNPKCRGK----LPPGSMGFPLLGESIQFFKPNKTSDIP-PFIKERVKKYGP--IFKTN 81

Query: 99  LYGKPTVIATDPEICRRIYLDEAK-FKQHNPESVKILEGSS--GDFSKMDHKIAYKVVAT 158
           L G+P +++TD ++   ++  E + F+   P++   + G    G      +K    +V T
Sbjct: 82  LVGRPVIVSTDADLSYFVFNQEGRCFQSWYPDTFTHIFGKKNVGSLHGFMYKYLKNMVLT 141

Query: 159 PMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKLLDEIGSLFFKVITKVFLGSRLDAK 218
            + G + L   +  +E    K LE WS+  ++ ++L D   S+ F +  K  +    D K
Sbjct: 142 -LFGHDGLKKMLPQVEMTANKRLELWSN--QDSVELKDATASMIFDLTAKKLISHDPD-K 201

Query: 219 TMVELHALYKELSFAMVMSTFPYDFPGFTFHQAFKARKKIENIIQGVVEEKRSRRFENDK 278
           +   L A +  ++F   + +FP+D PG  +H+  + R K   +++ +++E+R    EN +
Sbjct: 202 SSENLRANF--VAFIQGLISFPFDIPGTAYHKCLQGRAKAMKMLRNMLQERR----ENPR 261

Query: 279 TNEVQCQVDKMVVAIDENGEKLYNNNLIRDLLLRVLFAGHSTPAIAAFWALLHISQNPHV 338
            N      D ++  I + G  +    +  DL+  +LFA   T ++A   A+  +S +P V
Sbjct: 262 KNPSDF-FDYVIEEIQKEG-TILTEEIALDLMFVLLFASFETTSLALTLAIKFLSDDPEV 321

Query: 339 FQKAKEEQESIIRQRPSNKKGLTLNEIKQMKYLTKVINEVLRRNTITPTNFREAKTDVNI 398
            ++  EE E+I+R R     GLT  E K M Y  + INE  R   I P  FR+A  D+  
Sbjct: 322 LKRLTEEHETILRNREDADSGLTWEEYKSMTYTFQFINETARLANIVPAIFRKALRDIKF 381

Query: 399 NGYFIPKGWTIQIWNIAVHMDPQIYSNPQEFNPSRWE--NYTPKPGTFIPFGLGSRFCPG 458
             Y IP GW + +   AVH++P++Y +P  FNPSRWE    T     F+ FG G RFC G
Sbjct: 382 KDYTIPAGWAVMVCPPAVHLNPEMYKDPLVFNPSRWEGSKVTNASKHFMAFGGGMRFCVG 441

Query: 459 SELARLEITILLHHFILNYKY 475
           ++  +L++   LH  +  Y++
Sbjct: 442 TDFTKLQMAAFLHSLVTKYRW 443

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008466645.10.097.39PREDICTED: beta-amyrin 11-oxidase-like, partial [Cucumis melo][more]
XP_004148515.28.50e-30188.96beta-amyrin 11-oxidase [Cucumis sativus] >AIT72036.1 cytochrome P450 [Cucumis sa... [more]
XP_011652660.12.33e-23369.11beta-amyrin 11-oxidase [Cucumis sativus][more]
KAA0038545.11.75e-23197.06beta-amyrin 11-oxidase-like [Cucumis melo var. makuwa] >TYK31141.1 beta-amyrin 1... [more]
KAG6571834.11.78e-22267.32Beta-amyrin 11-oxidase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
B5BSX12.4e-10544.35Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1[more]
Q9C5Y24.0e-10044.62Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2[more]
Q5VRM74.5e-9637.66Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ... [more]
Q9AXH91.0e-9237.15Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1[more]
O230511.8e-9240.58Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3CRX80.097.39beta-amyrin 11-oxidase-like OS=Cucumis melo OX=3656 GN=LOC103504002 PE=3 SV=1[more]
A0A097IYP64.11e-30188.96Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa3G903550 PE=2 SV=1[more]
A0A5D3E6B28.47e-23297.06Beta-amyrin 11-oxidase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A6J1GJM11.47e-22167.10beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 S... [more]
A0A6J1GLR11.64e-22067.97beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455409 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT2G32440.12.8e-10144.62ent-kaurenoic acid hydroxylase 2 [more]
AT2G32440.22.8e-10144.62ent-kaurenoic acid hydroxylase 2 [more]
AT1G05160.11.3e-9340.58cytochrome P450, family 88, subfamily A, polypeptide 3 [more]
AT3G19270.12.0e-5431.87cytochrome P450, family 707, subfamily A, polypeptide 4 [more]
AT1G12740.19.8e-5429.93cytochrome P450, family 87, subfamily A, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 369..387
score: 29.16
coord: 441..451
score: 54.21
coord: 451..474
score: 27.84
coord: 409..433
score: 27.67
coord: 88..107
score: 20.64
coord: 198..216
score: 20.38
coord: 322..348
score: 25.71
IPR001128Cytochrome P450PRINTSPR00385P450coord: 442..451
score: 56.28
coord: 451..462
score: 41.8
coord: 370..381
score: 36.04
coord: 313..330
score: 27.65
IPR001128Cytochrome P450PFAMPF00067p450coord: 53..473
e-value: 1.7E-73
score: 247.9
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 46..474
e-value: 3.0E-91
score: 308.4
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 50..473
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 25..472
NoneNo IPR availablePANTHERPTHR24286:SF289ENT-KAURENOIC ACID OXIDASE 2coord: 25..472
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 444..453

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C003385.jh1.t1MELO3C003385.jh1.t1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen