MELO3C002580 (gene) Melon (DHL92) v4

Overview
NameMELO3C002580
Typegene
OrganismCucumis melo cv. DHL92 (Melon (DHL92) v4)
Descriptionpolyphenol oxidase, chloroplastic-like
Locationchr12: 21619714 .. 21621428 (-)
RNA-Seq ExpressionMELO3C002580
SyntenyMELO3C002580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCTCTATCTTCTCTTCTATCACTTTCTCCATATTCTCAATGCAAACCCCAGTCCTCCATTCCTTCAAAATACAAGGCATTAAGTATTAATATGTCTCATGCAAAGCCTCAAAAGGAGAAAAATCACCAAAACCCTCAGGCTCAAGGAGACTCTCAAATCAAATTTGATCGAAGAGATGTGTTGTTTGGACTTGGAGGTCTTTACGGCGTCACCACTCTCGGCAGCAATAAATTTGCGTTAGCTGCTGATGATGATTTGTCATTGTCACCGGATGTTCAAAACTGCGGACCGGCGGACACCCCTGTCGGCCCGATGGACTGTTGCCCCCCAAAAATCAACAAAATCATCGATTTCACTAAGCCAATCAACCCAAAACTTCATGTCCGACCAGCAGTCCAACTTTTAACACCAAAGCAACTCGCCGATTTCGAAAGAGGCATCGAAATAATGAAAAACCTTAAACCAGACGACCCTCGTAACTTTTATCAACAAGCTAAAATTCACTGTGCTTATTGTGATAGTGCTTATAAACAAATAGGCTCTGAACAAACTGATTTCCTGTTCATTTCAATGCCTTATTCTTCCCATTTCACCGTGCTTACCTTTACTACTTTGAAAGAATCTTACGCCATTTTATTGGTGACCCTGATTTTGCCATACCCTTTTGGAATTGGGATGCACCCATTGGCATGCACATGCCTATACATTACAACGGCAACCCAAATTCACCACTTTACGATGCGAATCGTAACCCCCTACACAAAGCACCAAAAGTTCTTGATCTTAACTATGACGGATCAACCGAAAACCCTCCTTATGATGTGGTGGATTGCAATCTCCGTTGGATGAATAAACAAATGAGTGTAAAAACCCCTACTCGGTTCCTTGGAAAAGTGTGCAAACTTGGGGAAACTCCAACTCTCGACGGCATGGGTACGATTGAAAGTTCTCCTCATAATAACGTTCACAATTGGGTCGGTAATTATCCGATAACTCCTAACGGTGAAGACATGGGAGCTTTTTATGCCGCAGCAAGAGATCCCATATTTTATGCTCACCATGCAAATGTGGATCGTGTGTGGTATATTTGGAACAAAATGAATTCTGGACGTATACCATACATTTACAGGCAAGATTGGTTGAATTCGACGTTTGTGTTTTATGACGAGCACCGAAACGCTGTACGTATCAAAGTTTCTGATTGTCTGGACAGTGAAAATCTTGGTTATGTTTATCAAGATGTAGAGATTCCATGGTTGAACACCAAGCAAGTCCCGCGGACTAAACGAAGTACAGGCCAACCCGAGCCGCCGGTTATTAACTTCCCGCAGACTCTGAGCTCGGCCATAACCACATCAGTGGAAAGGCCAAGTGGTGTAGAGCCACAGGAAAATGAAGAAGAAGTTTTGGTGATTGAAGATATTGAGTATGATCCAACTAAGAATCTGCATTTTAATGTGCTTGTTGACGTTGTTGATGAAGGGGATGATGTTTGTCCAGGGAATGTTGAGTTTCTGGGAAGCTTTAAGAACATCCCACACGGTTCAAAGCACGAAGCGAAGTCCTCGTTGCGTTTCGTGTTAACTGAGGCAATTGAAGCTTTGGGTTTGCATGATGCTCGGAAGTTGGCTGTGAGGATTGACCCTAAGCTTAATGGAGATCAAATTAAAGTCGGAGGGATCAAGATTCAGATTGAACCTATCGAATAA

mRNA sequence

ATGGCTTCTCTATCTTCTCTTCTATCACTTTCTCCATATTCTCAATGCAAACCCCAGTCCTCCATTCCTTCAAAATACAAGGCATTAAGTATTAATATGTCTCATGCAAAGCCTCAAAAGGAGAAAAATCACCAAAACCCTCAGGCTCAAGGAGACTCTCAAATCAAATTTGATCGAAGAGATGTGTTGTTTGGACTTGGAGGTCTTTACGGCGTCACCACTCTCGGCAGCAATAAATTTGCGTTAGCTGCTGATGATGATTTGTCATTGTCACCGGATGTTCAAAACTGCGGACCGGCGGACACCCCTGTCGGCCCGATGGACTGTTGCCCCCCAAAAATCAACAAAATCATCGATTTCACTAAGCCAATCAACCCAAAACTTCATGTCCGACCAGCAGTCCAACTTTTAACACCAAAGCAACTCGCCGATTTCGAAAGAGGCATCGAAATAATGAAAAACCTTAAACCAGACGACCCTCGTAACTTTTATCAACAAGCTAAAATTCACTGTGCTTATTGTGATAGTGCTTATAAACAAATAGGCTCTGAACAAACTGATTTCCTAATCTTACGCCATTTTATTGGTGACCCTGATTTTGCCATACCCTTTTGGAATTGGGATGCACCCATTGGCATGCACATGCCTATACATTACAACGGCAACCCAAATTCACCACTTTACGATGCGAATCGTAACCCCCTACACAAAGCACCAAAAGTTCTTGATCTTAACTATGACGGATCAACCGAAAACCCTCCTTATGATGTGGTGGATTGCAATCTCCGTTGGATGAATAAACAAATGAGTGTAAAAACCCCTACTCGGTTCCTTGGAAAAGTGTGCAAACTTGGGGAAACTCCAACTCTCGACGGCATGGGTACGATTGAAAGTTCTCCTCATAATAACGTTCACAATTGGGTCGGTAATTATCCGATAACTCCTAACGGTGAAGACATGGGAGCTTTTTATGCCGCAGCAAGAGATCCCATATTTTATGCTCACCATGCAAATGTGGATCGTGTGTGGTATATTTGGAACAAAATGAATTCTGGACGTATACCATACATTTACAGGCAAGATTGGTTGAATTCGACGTTTGTGTTTTATGACGAGCACCGAAACGCTGTACGTATCAAAGTTTCTGATTGTCTGGACAGTGAAAATCTTGGTTATGTTTATCAAGATGTAGAGATTCCATGGTTGAACACCAAGCAAGTCCCGCGGACTAAACGAAGTACAGGCCAACCCGAGCCGCCGGTTATTAACTTCCCGCAGACTCTGAGCTCGGCCATAACCACATCAGTGGAAAGGCCAAGTGGTGTAGAGCCACAGGAAAATGAAGAAGAAGTTTTGGTGATTGAAGATATTGAGTATGATCCAACTAAGAATCTGCATTTTAATGTGCTTGTTGACGTTGTTGATGAAGGGGATGATGTTTGTCCAGGGAATGTTGAGTTTCTGGGAAGCTTTAAGAACATCCCACACGGTTCAAAGCACGAAGCGAAGTCCTCGTTGCGTTTCGTGTTAACTGAGGCAATTGAAGCTTTGGGTTTGCATGATGCTCGGAAGTTGGCTGTGAGGATTGACCCTAAGCTTAATGGAGATCAAATTAAAGTCGGAGGGATCAAGATTCAGATTGAACCTATCGAATAA

Coding sequence (CDS)

ATGGCTTCTCTATCTTCTCTTCTATCACTTTCTCCATATTCTCAATGCAAACCCCAGTCCTCCATTCCTTCAAAATACAAGGCATTAAGTATTAATATGTCTCATGCAAAGCCTCAAAAGGAGAAAAATCACCAAAACCCTCAGGCTCAAGGAGACTCTCAAATCAAATTTGATCGAAGAGATGTGTTGTTTGGACTTGGAGGTCTTTACGGCGTCACCACTCTCGGCAGCAATAAATTTGCGTTAGCTGCTGATGATGATTTGTCATTGTCACCGGATGTTCAAAACTGCGGACCGGCGGACACCCCTGTCGGCCCGATGGACTGTTGCCCCCCAAAAATCAACAAAATCATCGATTTCACTAAGCCAATCAACCCAAAACTTCATGTCCGACCAGCAGTCCAACTTTTAACACCAAAGCAACTCGCCGATTTCGAAAGAGGCATCGAAATAATGAAAAACCTTAAACCAGACGACCCTCGTAACTTTTATCAACAAGCTAAAATTCACTGTGCTTATTGTGATAGTGCTTATAAACAAATAGGCTCTGAACAAACTGATTTCCTAATCTTACGCCATTTTATTGGTGACCCTGATTTTGCCATACCCTTTTGGAATTGGGATGCACCCATTGGCATGCACATGCCTATACATTACAACGGCAACCCAAATTCACCACTTTACGATGCGAATCGTAACCCCCTACACAAAGCACCAAAAGTTCTTGATCTTAACTATGACGGATCAACCGAAAACCCTCCTTATGATGTGGTGGATTGCAATCTCCGTTGGATGAATAAACAAATGAGTGTAAAAACCCCTACTCGGTTCCTTGGAAAAGTGTGCAAACTTGGGGAAACTCCAACTCTCGACGGCATGGGTACGATTGAAAGTTCTCCTCATAATAACGTTCACAATTGGGTCGGTAATTATCCGATAACTCCTAACGGTGAAGACATGGGAGCTTTTTATGCCGCAGCAAGAGATCCCATATTTTATGCTCACCATGCAAATGTGGATCGTGTGTGGTATATTTGGAACAAAATGAATTCTGGACGTATACCATACATTTACAGGCAAGATTGGTTGAATTCGACGTTTGTGTTTTATGACGAGCACCGAAACGCTGTACGTATCAAAGTTTCTGATTGTCTGGACAGTGAAAATCTTGGTTATGTTTATCAAGATGTAGAGATTCCATGGTTGAACACCAAGCAAGTCCCGCGGACTAAACGAAGTACAGGCCAACCCGAGCCGCCGGTTATTAACTTCCCGCAGACTCTGAGCTCGGCCATAACCACATCAGTGGAAAGGCCAAGTGGTGTAGAGCCACAGGAAAATGAAGAAGAAGTTTTGGTGATTGAAGATATTGAGTATGATCCAACTAAGAATCTGCATTTTAATGTGCTTGTTGACGTTGTTGATGAAGGGGATGATGTTTGTCCAGGGAATGTTGAGTTTCTGGGAAGCTTTAAGAACATCCCACACGGTTCAAAGCACGAAGCGAAGTCCTCGTTGCGTTTCGTGTTAACTGAGGCAATTGAAGCTTTGGGTTTGCATGATGCTCGGAAGTTGGCTGTGAGGATTGACCCTAAGCTTAATGGAGATCAAATTAAAGTCGGAGGGATCAAGATTCAGATTGAACCTATCGAATAA

Protein sequence

MASLSSLLSLSPYSQCKPQSSIPSKYKALSINMSHAKPQKEKNHQNPQAQGDSQIKFDRRDVLFGLGGLYGVTTLGSNKFALAADDDLSLSPDVQNCGPADTPVGPMDCCPPKINKIIDFTKPINPKLHVRPAVQLLTPKQLADFERGIEIMKNLKPDDPRNFYQQAKIHCAYCDSAYKQIGSEQTDFLILRHFIGDPDFAIPFWNWDAPIGMHMPIHYNGNPNSPLYDANRNPLHKAPKVLDLNYDGSTENPPYDVVDCNLRWMNKQMSVKTPTRFLGKVCKLGETPTLDGMGTIESSPHNNVHNWVGNYPITPNGEDMGAFYAAARDPIFYAHHANVDRVWYIWNKMNSGRIPYIYRQDWLNSTFVFYDEHRNAVRIKVSDCLDSENLGYVYQDVEIPWLNTKQVPRTKRSTGQPEPPVINFPQTLSSAITTSVERPSGVEPQENEEEVLVIEDIEYDPTKNLHFNVLVDVVDEGDDVCPGNVEFLGSFKNIPHGSKHEAKSSLRFVLTEAIEALGLHDARKLAVRIDPKLNGDQIKVGGIKIQIEPIE
Homology
BLAST of MELO3C002580 vs. ExPASy Swiss-Prot
Match: P43309 (Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 2.6e-111
Identity = 243/585 (41.54%), Postives = 328/585 (56.07%), Query Frame = 0

Query: 10  LSPYSQCKPQSSIPSKYKALSINMSHAKPQKEKNHQNPQAQGDSQIKFDRRDVLFGLGGL 69
           LSP+SQ   Q S+ +K K         K     N QN QAQ     K DRR+VL GLGGL
Sbjct: 22  LSPFSQNNSQVSLLTKPKRSFARKVSCK--ATNNDQNDQAQS----KLDRRNVLLGLGGL 81

Query: 70  YGVTTLGSNKFALAADDDLSLSPDVQNCGPADTPVG--PMDCCPPKINKIIDFTKPINPK 129
           YGV  +G++ FA A        PDV  CGPAD P G  P +CCPP   KIIDF  P   K
Sbjct: 82  YGVAGMGTDPFAFAKP---IAPPDVSKCGPADLPQGAVPTNCCPPPSTKIIDFKLPAPAK 141

Query: 130 LHVRPAVQLLTPKQLADFERGIEIMKNLKPDDPRNFYQQAKIHCAYCDSAYKQIGSEQTD 189
           L +RP    +       + + +E+MK L  DDPR+F QQA +HCAYCD AY Q+G  + +
Sbjct: 142 LRIRPPAHAVDQAYRDKYYKAMELMKALPDDDPRSFKQQAAVHCAYCDGAYDQVGFPELE 201

Query: 190 F--------------------LILRHFIGDPDFAIPFWNWDAPIGMHMPIHYNGNPNSPL 249
                                 IL   I DP FA+PFWNWD+P GM +P  Y  +P SPL
Sbjct: 202 LQIHNSWLFFPFHRYYLYFFEKILGKLINDPTFALPFWNWDSPAGMPLPAIY-ADPKSPL 261

Query: 250 YDANRNPLHKAPKVLDLNYDGSTENPPYD-VVDCNLRWMNKQM--SVKTPTRFLGKVCKL 309
           YD  R+  H+ P ++DL+Y+G+ +N   +  ++ NL+ M +QM  + K    F G   + 
Sbjct: 262 YDKLRSANHQPPTLVDLDYNGTEDNVSKETTINANLKIMYRQMVSNSKNAKLFFGNPYRA 321

Query: 310 GETPTLDGMGTIESSPHNNVHNWVGNYPITPNGEDMGAFYAAARDPIFYAHHANVDRVWY 369
           G+ P   G G+IE +PH  VH W G+    PN EDMG FY+A RDPIF+AHH+NVDR+W 
Sbjct: 322 GDEPD-PGGGSIEGTPHAPVHLWTGD-NTQPNFEDMGNFYSAGRDPIFFAHHSNVDRMWS 381

Query: 370 IWNKMNSGRIPYIYRQDWLNSTFVFYDEHRNAVRIKVSDCLDSENLGYVYQDVEIPWLNT 429
           IW K   G+   +   DWL+S F+FY+E+   VR+KV DCL+++NLGYVYQDV+IPWL++
Sbjct: 382 IW-KTLGGKRTDLTDSDWLDSGFLFYNENAELVRVKVRDCLETKNLGYVYQDVDIPWLSS 441

Query: 430 KQVPR--------TKRSTGQPEPPVIN---------FPQTLSSAITTSVERPS----GVE 489
           K  PR          +  G     V +         FP +L S I+T V+RP       +
Sbjct: 442 KPTPRRAKVALSKVAKKLGVAHAAVASSSKVVAGTEFPISLGSKISTVVKRPKQKKRSKK 501

Query: 490 PQENEEEVLVIEDIEYDPTKNLHFNVLVDVVDEGDDVCPGNVEFLGSFKNIPHGSKHEAK 547
            +E+EEE+LVIE IE+D    + F+V V+ VD+     P   EF GSF ++PH  KH+ K
Sbjct: 502 AKEDEEEILVIEGIEFDRDVAVKFDVYVNDVDDLPS-GPDKTEFAGSFVSVPHSHKHKKK 561

BLAST of MELO3C002580 vs. ExPASy Swiss-Prot
Match: Q6UIL3 ((+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata OX=66636 PE=1 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 3.4e-103
Identity = 239/592 (40.37%), Postives = 329/592 (55.57%), Query Frame = 0

Query: 1   MASLSSLLSL--SPYSQCKPQSSIPSKYKALSINMSHAKPQKEKNHQNPQAQGDSQIKFD 60
           MASLSS   L  +PYS   P  + PS+     ++   +   K+K    P  Q +     D
Sbjct: 1   MASLSSQSKLLATPYS--FPYHTKPSRVSLRRVSCKASNDNKDK----PNDQ-EKTFSID 60

Query: 61  RRDVLFGLGGLYGVTTL-GSNKFALAADDDLSLSPDVQNCGPADTPVGPMD--CCPPKIN 120
           RR++L GLGGLYG + +  SN+  LAA     + PD  +C P +     ++  CCPP + 
Sbjct: 61  RRNMLIGLGGLYGASNVFPSNQSTLAAPIQPPVLPD--SCHPPEDLAEGVNVLCCPPDVK 120

Query: 121 KIIDFTKPINP-KLHVRPAVQLLTPKQLADFERGIEIMKNLKPDDPRNFYQQAKIHCAYC 180
             IDF  P NP +L +RPA  L  P  +  +++ +  MK L  +DPR+FYQQA IHCAYC
Sbjct: 121 DPIDFQMPSNPSRLRIRPAAHLADPTYIEKYKKALAAMKALPQNDPRSFYQQANIHCAYC 180

Query: 181 DSAYKQIGSEQTD--------FL------------ILRHFIGDPDFAIPFWNWDAPIGMH 240
           + AY Q+G    +        FL            IL   I DP FAIPFWNWDAP GMH
Sbjct: 181 NGAYDQVGFPDVNIQVHHSWLFLPFHRWYLYFYERILGSLIDDPTFAIPFWNWDAPKGMH 240

Query: 241 MPIHYNGNPNSPLYDANRNPLHKAPKVLDLNYDGSTENPPYD--VVDCNLRWMNKQ-MSV 300
           MP H   +PNSPLYDA RNP H    ++DL++  S E P ++   +  NL  M KQ +  
Sbjct: 241 MP-HMFIDPNSPLYDAKRNPAHFPDTIVDLDF-SSGEAPSHNPRQIGNNLSIMYKQVVRA 300

Query: 301 KTPTRFLGKVCKLGETPTLDGMGTIESSPHNNVHNWVGNYPITPNGEDMGAFYAAARDPI 360
           K    F G+  + G  P   G G++E +PH N+H W G+ P   N E+MG FY+A RDP+
Sbjct: 301 KKARLFHGRPLQAGSFPDESGDGSLEGTPHGNIHLWSGD-PRQSNFENMGNFYSAGRDPL 360

Query: 361 FYAHHANVDRVWYIWNKMNSGRIPYIYRQDWLNSTFVFYDEHRNAVRIKVSDCLDSENLG 420
           FYAHHANVDR+WYIW K + GR  Y  ++DWLN+ F+ +DE+   VR+ V D LD   LG
Sbjct: 361 FYAHHANVDRMWYIW-KNSLGRKDY-KKKDWLNAGFLLFDENAQPVRVYVRDALDERKLG 420

Query: 421 YVYQDVEIPWLNTKQVPRTK-------RSTGQPEPPVIN-FPQTLSSAITTSVERPS--- 480
           Y YQ+V+IPW+N+K  PR         RS       +IN FP TL S ++  V+RP    
Sbjct: 421 YAYQEVDIPWINSKPKPRKANPWPNRLRSKATTTTKLINKFPLTLDSTVSFEVKRPKKSR 480

Query: 481 GVEPQENEEEVLVIEDIEYDPTKNLHFNVLVDVVDEGDDVCPGNVEFLGSFKNIPHGSKH 540
               +E+EEEVLVIE I+++P   L F+V ++  DE D       EF GSF N+PH  +H
Sbjct: 481 SKSEKEDEEEVLVIEKIKHEPQFPLKFDVYINDEDE-DPSAADQTEFAGSFVNVPHFHRH 540

Query: 541 EAK------SSLRFVLTEAIEALGLHDARKLAVRIDPKLNGDQIKVGGIKIQ 547
             K      ++L   ++E ++ L +     + V + P++   QI +GG KI+
Sbjct: 541 GDKKDKRQTTNLSIGISEVLDELDVDGDDSIVVTLVPRVGSGQITIGGAKIE 577

BLAST of MELO3C002580 vs. ExPASy Swiss-Prot
Match: P43311 (Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 1.9e-101
Identity = 233/582 (40.03%), Postives = 315/582 (54.12%), Query Frame = 0

Query: 12  PYSQCKPQSSIPSKYKALSINMSHAKPQKEKNHQNPQAQGDSQIKFDRRDVLFGLGGLYG 71
           P ++ + +   PSK   +S N ++  P  +        +  S  K DRR+VL G+GGLYG
Sbjct: 36  PVARNRSRRFAPSK---VSCNSANGDPNSD---STSDVRETSSGKLDRRNVLLGIGGLYG 95

Query: 72  VT-TLGSNKFALAADDDLSLSPDVQNCGPADTPVG--PMDCCPPKINKIIDFTKPIN-PK 131
               LG+ K  LA    +  +PD+  CG A  P G  P +CCPP   KIIDF  P +   
Sbjct: 96  AAGGLGATK-PLAFGAPIQ-APDISKCGTATVPDGVTPTNCCPPVTTKIIDFQLPSSGSP 155

Query: 132 LHVRPAVQLLTPKQLADFERGIEIMKNLKPDDPRNFYQQAKIHCAYCDSAYKQIGSEQTD 191
           +  RPA  L++ + LA +++ IE+ K L  DDPR+F QQA +HC YC  AY Q+G    +
Sbjct: 156 MRTRPAAHLVSKEYLAKYKKAIELQKALPDDDPRSFKQQANVHCTYCQGAYDQVGYTDLE 215

Query: 192 --------FL------------ILRHFIGDPDFAIPFWNWDAPIGMHMPIHYNGNPNSPL 251
                   FL            IL   I DP FA+P+W WD P GM+MP  Y  +P+S L
Sbjct: 216 LQVHASWLFLPFHRYYLYFNERILAKLIDDPTFALPYWAWDNPDGMYMPTIYASSPSS-L 275

Query: 252 YDANRNPLHKAPKVLDLNYDGSTENPPYDVVDC-NLRWMNKQM--SVKTPTRFLGKVCKL 311
           YD  RN  H  P V+DL+YDG+    P D +   NL  M KQ+     TP  FLG   + 
Sbjct: 276 YDEKRNAKHLPPTVIDLDYDGTEPTIPDDELKTDNLAIMYKQIVSGATTPKLFLGYPYRA 335

Query: 312 GETPTLDGMGTIESSPHNNVHNWVGNYPITPNGEDMGAFYAAARDPIFYAHHANVDRVWY 371
           G+     G GT+E +PHN VH W G        EDMG FY A RDPIF+ HHANVDR+W 
Sbjct: 336 GDAID-PGAGTLEHAPHNIVHKWTG--LADKPSEDMGNFYTAGRDPIFFGHHANVDRMWN 395

Query: 372 IWNKMNSGRIPYIYRQDWLNSTFVFYDEHRNAVRIKVSDCLDSENLGYVYQDVEIPWL-- 431
           IW  +           DWL++TFVFYDE++  V++KVSDC+D+  L Y YQD+ IPWL  
Sbjct: 396 IWKTIGGKNRKDFTDTDWLDATFVFYDENKQLVKVKVSDCVDTSKLRYQYQDIPIPWLPK 455

Query: 432 NTKQVPRTKRSTGQ------PEPP------VINFPQTLSSAITTSVERPS---GVEPQEN 491
           NTK   +T   + +       E P      + +FP+ L+S I   V RP      + +E+
Sbjct: 456 NTKAKAKTTTKSSKSGVAKAAELPKTTISSIGDFPKALNSVIRVEVPRPKKSRSKKEKED 515

Query: 492 EEEVLVIEDIEYDPTKNLHFNVLVDVVDEGDDVC-PGNVEFLGSFKNIPHGSKHE--AKS 547
           EEEVL+I+ IE D    + F+V ++  DE   V  P N EF GSF N+PH    E   K+
Sbjct: 516 EEEVLLIKGIELDRENFVKFDVYIN--DEDYSVSRPKNSEFAGSFVNVPHKHMKEMKTKT 575

BLAST of MELO3C002580 vs. ExPASy Swiss-Prot
Match: Q9MB14 (Polyphenol oxidase II, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-2 PE=2 SV=2)

HSP 1 Score: 367.9 bits (943), Expect = 2.1e-100
Identity = 222/537 (41.34%), Postives = 299/537 (55.68%), Query Frame = 0

Query: 51  GDSQIKFDRRDVLFGLGGLYGVTTLGSNKFALAADDDLSLSPDVQNC--GPADTPVGPM- 110
           GDSQ K DRRDVL GLGGL G  +L +N  A A   +   +P++  C   P D P   + 
Sbjct: 56  GDSQPKLDRRDVLLGLGGLAGAASLINNPLAFA---EPIHAPEISKCVVPPKDLPPDAIV 115

Query: 111 -DCCPPKINKIIDFTKP-INPK-LHVRPAVQLLTPKQLADFERGIEIMKNLKPDDPRNFY 170
            +CCPP    +I +  P  +P  + +RPA+  +  + +A FE+ I +MK L  DDPRNFY
Sbjct: 116 DNCCPPLATNVIPYKVPKTSPSAMKIRPAIHRMDKEYIAKFEKAIRLMKELPADDPRNFY 175

Query: 171 QQAKIHCAYCDSAYKQIGSEQTDF--------------------LILRHFIGDPDFAIPF 230
           QQA +HCAYC+  Y Q      +                      IL   IGDP F +PF
Sbjct: 176 QQALVHCAYCNGGYVQTDYPDKEIQVHNSWLFFPFHRWYLYFYERILGKLIGDPTFGLPF 235

Query: 231 WNWDAPIGMHMPIHYNGNPNSPLYDANRNPLHKAPKVLDLNYDGS-TENPPYDVVDCNLR 290
           WNWD P GM +P  Y  N NSPLYD NRN  H  P V+DL Y G+ T+    + +  NL 
Sbjct: 236 WNWDTPAGMLIP-QYFRNQNSPLYDENRNQSH-LPLVMDLGYAGTDTDVTDQERISNNLA 295

Query: 291 WMNKQM--SVKTPTRFLGKVCKLGETPTLDGMGTIESSPHNNVHNWVGNY-PITPNGEDM 350
            M K M  +  T   FLGK  K G+ P   G G+IE+ PH  VH WVG+  P T NGEDM
Sbjct: 296 LMYKSMVTNAGTAELFLGKPYKAGDDPVNKGGGSIENIPHTPVHRWVGDVKPRTQNGEDM 355

Query: 351 GAFYAAARDPIFYAHHANVDRVWYIWNKM-NSGRIPYIYRQDWLNSTFVFYDEHRNAVRI 410
           G FY+A RD +FY HH+NVDR+W IW ++   GR       DWL++TF+FYDE++ AVR+
Sbjct: 356 GNFYSAGRDILFYCHHSNVDRMWTIWQQLGGKGRRRDFTDSDWLDATFIFYDENKQAVRV 415

Query: 411 KVSDCLDSENLGYVYQDVEIPWLNTKQVPRTKRSTG---QPEPPVIN--FPQTLSSAITT 470
           +V D LD++ LGY Y+   +PWLN+K +P TK+ TG   + + P +   FP TL   +  
Sbjct: 416 RVGDALDNQKLGYKYEFTNLPWLNSKPLP-TKKKTGLAARSKAPFVTDVFPLTLDKVVQV 475

Query: 471 SVERPS---GVEPQENEEEVLVIEDIEYDPTKNLHFNVLVDVVDEGDDVCPGNVEFLGSF 530
            V RP      E +E EEE+L I+ IE    +   F+V ++  DE  +      E+ GSF
Sbjct: 476 KVPRPKKSRSKEEKEAEEEILEIQGIEVAIDQYAKFDVYLNDEDE-PEAGKEKAEYAGSF 535

Query: 531 KNIPH---GSKHEAKSSLRFVLTEAIEALGLHDARKLAVRIDPKLNGDQIKVGGIKI 546
            ++PH   GSK + ++SL   L E +E LG  D   + V + PK+ G  + V  IKI
Sbjct: 536 AHLPHKHTGSK-KIRTSLSLGLNEPLEDLGAEDDDAVLVTLAPKVGGGVVTVENIKI 584

BLAST of MELO3C002580 vs. ExPASy Swiss-Prot
Match: O81103 (Polyphenol oxidase latent form, chloroplastic OS=Prunus armeniaca OX=36596 GN=PPO PE=1 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 1.1e-98
Identity = 227/593 (38.28%), Postives = 321/593 (54.13%), Query Frame = 0

Query: 1   MASLSSLLSLSPYSQCKP---QSSIPSKYKALSINMS----HAKPQKEKNHQNPQAQGDS 60
           M + SSL+S + +S   P   Q S+  K K      S     A      N+QN Q +  S
Sbjct: 10  MGTYSSLISTNSFSTFLPNKSQLSLSGKSKHYVARRSSISCKATNNNNSNNQNEQQEESS 69

Query: 61  QI--KFDRRDVLFGLGGLYGVTTLGSNKFALAADDDLSLSPDVQNCGPAD-TPVG--PMD 120
           ++  K DRR++L GLGGLYG TTL    FA A   D    PD+  C PA+ TP G   + 
Sbjct: 70  RLLGKLDRRNILIGLGGLYGATTLDRKPFAFA---DPIAPPDLTTCKPAEITPGGSETVP 129

Query: 121 CCPPKINKIIDFTKPINPKLHVRPAVQLLTPKQLADFERGIEIMKNLKPDDPRNFYQQAK 180
           CCPP   KI  F   ++  L   PA   +T + LA F++    M+ L  DDPR+  QQAK
Sbjct: 130 CCPPVTTKIKTFKPDLSIPLRTSPAAHQVTDEYLAKFKKAQAAMRALPDDDPRSMVQQAK 189

Query: 181 IHCAYCDSAYKQIGSEQTDF--------------------LILRHFIGDPDFAIPFWNWD 240
           +HCAYC+ AY Q+G    D                      IL   I DP FA+P+WNWD
Sbjct: 190 VHCAYCNGAYPQVGFTDNDIQVHFSWLFFPFHRMYLYFYERILGKLIDDPTFALPYWNWD 249

Query: 241 APIGMHMPIHYNGNPNSPLYDANRNPLHKAPKVLDLNYDGSTENPPYDV-VDCNLRWMNK 300
           +P+G  +P  Y  + +SPLYD  RN  H+ P ++DL+Y G  ++      +D NL  M +
Sbjct: 250 SPVGFPIPDIYT-DTSSPLYDQYRNADHQPPVLVDLSYGGKDDDVDEQTRIDENLAIMYR 309

Query: 301 QM--SVKTPTRFLGKVCKLGETPTLDGMGTIESSPHNNVHNWVGNYPITPNGEDMGAFYA 360
           QM    KTP  F G   + G   T    GTIE+ PHNN+H WVG+ P   + EDMG FY+
Sbjct: 310 QMVSGAKTPDLFFGHAYRAGNLNTGKYPGTIENMPHNNIHIWVGD-PSQTHQEDMGNFYS 369

Query: 361 AARDPIFYAHHANVDRVWYIWNKMNSGRIPYIYRQDWLNSTFVFYDEHRNAVRIKVSDCL 420
           A RDP+FYAHHANVDR+W IW K   G+   I   DWL++ F+FYDE+   VR+KV D L
Sbjct: 370 AGRDPLFYAHHANVDRMWNIW-KTLGGKRKDITDTDWLDAEFLFYDENAELVRVKVRDSL 429

Query: 421 DSE-NLGYVYQDVEIPWLNTKQVPRTKRSTGQPEPPVI----NFPQTLSSAITTSVERPS 480
           + E  L Y Y+ V +PWL TK   R  ++  + +         FP TL    T  V RP 
Sbjct: 430 EPEKQLRYNYEPVSLPWLFTKPTARKTKNKTKAKVAATQLTSKFPATLVEVTTVEVARPK 489

Query: 481 ----GVEPQENEEEVLVIEDIEYDPTKNLHFNVLVDVVDEGDDVC-PGNVEFLGSFKNIP 540
                 + + +EEE+L+I+DIE++ T+ + F+V ++  D+ + +      EF GSF ++P
Sbjct: 490 PRKRSKKEKVDEEELLIIKDIEFEGTEAVKFDVFIN--DDAESLSRRDKSEFAGSFVHVP 549

Query: 541 HG--SKHEAKSSLRFVLTEAIEALGLHDARKLAVRIDPKLNGDQIKVGGIKIQ 547
            G  +K + K++L+  +T+ +E LG  D   + V + P+++   I +GG KI+
Sbjct: 550 QGKTTKAKTKTNLKLGITDLLEDLGAEDDSSVLVTLVPRVSNSPITIGGFKIE 594

BLAST of MELO3C002580 vs. NCBI nr
Match: XP_008446638.1 (PREDICTED: polyphenol oxidase, chloroplastic-like [Cucumis melo] >KAA0056970.1 polyphenol oxidase [Cucumis melo var. makuwa] >TYK26396.1 polyphenol oxidase [Cucumis melo var. makuwa])

HSP 1 Score: 1149.8 bits (2973), Expect = 0.0e+00
Identity = 551/551 (100.00%), Postives = 551/551 (100.00%), Query Frame = 0

Query: 1   MASLSSLLSLSPYSQCKPQSSIPSKYKALSINMSHAKPQKEKNHQNPQAQGDSQIKFDRR 60
           MASLSSLLSLSPYSQCKPQSSIPSKYKALSINMSHAKPQKEKNHQNPQAQGDSQIKFDRR
Sbjct: 1   MASLSSLLSLSPYSQCKPQSSIPSKYKALSINMSHAKPQKEKNHQNPQAQGDSQIKFDRR 60

Query: 61  DVLFGLGGLYGVTTLGSNKFALAADDDLSLSPDVQNCGPADTPVGPMDCCPPKINKIIDF 120
           DVLFGLGGLYGVTTLGSNKFALAADDDLSLSPDVQNCGPADTPVGPMDCCPPKINKIIDF
Sbjct: 61  DVLFGLGGLYGVTTLGSNKFALAADDDLSLSPDVQNCGPADTPVGPMDCCPPKINKIIDF 120

Query: 121 TKPINPKLHVRPAVQLLTPKQLADFERGIEIMKNLKPDDPRNFYQQAKIHCAYCDSAYKQ 180
           TKPINPKLHVRPAVQLLTPKQLADFERGIEIMKNLKPDDPRNFYQQAKIHCAYCDSAYKQ
Sbjct: 121 TKPINPKLHVRPAVQLLTPKQLADFERGIEIMKNLKPDDPRNFYQQAKIHCAYCDSAYKQ 180

Query: 181 IGSEQTDFLILRHFIGDPDFAIPFWNWDAPIGMHMPIHYNGNPNSPLYDANRNPLHKAPK 240
           IGSEQTDFLILRHFIGDPDFAIPFWNWDAPIGMHMPIHYNGNPNSPLYDANRNPLHKAPK
Sbjct: 181 IGSEQTDFLILRHFIGDPDFAIPFWNWDAPIGMHMPIHYNGNPNSPLYDANRNPLHKAPK 240

Query: 241 VLDLNYDGSTENPPYDVVDCNLRWMNKQMSVKTPTRFLGKVCKLGETPTLDGMGTIESSP 300
           VLDLNYDGSTENPPYDVVDCNLRWMNKQMSVKTPTRFLGKVCKLGETPTLDGMGTIESSP
Sbjct: 241 VLDLNYDGSTENPPYDVVDCNLRWMNKQMSVKTPTRFLGKVCKLGETPTLDGMGTIESSP 300

Query: 301 HNNVHNWVGNYPITPNGEDMGAFYAAARDPIFYAHHANVDRVWYIWNKMNSGRIPYIYRQ 360
           HNNVHNWVGNYPITPNGEDMGAFYAAARDPIFYAHHANVDRVWYIWNKMNSGRIPYIYRQ
Sbjct: 301 HNNVHNWVGNYPITPNGEDMGAFYAAARDPIFYAHHANVDRVWYIWNKMNSGRIPYIYRQ 360

Query: 361 DWLNSTFVFYDEHRNAVRIKVSDCLDSENLGYVYQDVEIPWLNTKQVPRTKRSTGQPEPP 420
           DWLNSTFVFYDEHRNAVRIKVSDCLDSENLGYVYQDVEIPWLNTKQVPRTKRSTGQPEPP
Sbjct: 361 DWLNSTFVFYDEHRNAVRIKVSDCLDSENLGYVYQDVEIPWLNTKQVPRTKRSTGQPEPP 420

Query: 421 VINFPQTLSSAITTSVERPSGVEPQENEEEVLVIEDIEYDPTKNLHFNVLVDVVDEGDDV 480
           VINFPQTLSSAITTSVERPSGVEPQENEEEVLVIEDIEYDPTKNLHFNVLVDVVDEGDDV
Sbjct: 421 VINFPQTLSSAITTSVERPSGVEPQENEEEVLVIEDIEYDPTKNLHFNVLVDVVDEGDDV 480

Query: 481 CPGNVEFLGSFKNIPHGSKHEAKSSLRFVLTEAIEALGLHDARKLAVRIDPKLNGDQIKV 540
           CPGNVEFLGSFKNIPHGSKHEAKSSLRFVLTEAIEALGLHDARKLAVRIDPKLNGDQIKV
Sbjct: 481 CPGNVEFLGSFKNIPHGSKHEAKSSLRFVLTEAIEALGLHDARKLAVRIDPKLNGDQIKV 540

Query: 541 GGIKIQIEPIE 552
           GGIKIQIEPIE
Sbjct: 541 GGIKIQIEPIE 551

BLAST of MELO3C002580 vs. NCBI nr
Match: KAA0056962.1 (polyphenol oxidase I [Cucumis melo var. makuwa] >TYK26390.1 polyphenol oxidase I [Cucumis melo var. makuwa])

HSP 1 Score: 593.6 bits (1529), Expect = 1.8e-165
Identity = 308/574 (53.66%), Postives = 386/574 (67.25%), Query Frame = 0

Query: 1   MASLSSLLSLSPYSQCKPQSSIPSKYKALSINMSHAKPQKEKNHQNPQAQGDSQIKFDRR 60
           MASLSSLL LSPYSQ K + SI S  + + +N    K     N +      DS  KFDRR
Sbjct: 1   MASLSSLLLLSPYSQTKTKFSISSNKRHVVVNYVSCKAASSNNGE------DSVSKFDRR 60

Query: 61  DVLFGLGGLYGVTTLGSNKFALAADDDLSLSPDVQNCGPADTPVGPMDCCPPKINKIIDF 120
           +VLFGLGGLYGV T   +  A        L+PDV NC  A TP G  +CCPP+I KII F
Sbjct: 61  EVLFGLGGLYGVVTATGSVLA------APLTPDVFNCDMAQTPGGIKNCCPPRIGKIIHF 120

Query: 121 TKPINPKLHV-RPAVQLLTPKQLADFERGIEIMKNLKPDDPRNFYQQAKIHCAYCDSAYK 180
             P   + +V RPA  L+   Q+ + ERGI+ +K L  DDP +F  QAKIHCAYCD AY 
Sbjct: 121 VPPKIQQPYVRRPAAHLMNQTQIEELERGIQKLKQLDEDDPHSFSHQAKIHCAYCDYAYN 180

Query: 181 QIGS-EQTDF--------------------LILRHFIGDPDFAIPFWNWDAPIGMHMPIH 240
           Q+GS  +T F                     ILRH+IG+PDFAIPFWNWD+P GMHMP  
Sbjct: 181 QLGSITETRFEVHSNALFFPFHRAYLYFFERILRHYIGNPDFAIPFWNWDSPPGMHMPEF 240

Query: 241 YNGNPNSPLYDANRNPLHKAPKVLDLNYDGSTENPPYDVVDCNLRWMNKQMSVKTPTRFL 300
           YN  P+S LYD  R+P H   K++DLNY+G TE+  +DVVDCNLRWMN +M V++P  FL
Sbjct: 241 YN-RPSSSLYDNLRDPRHDPHKLIDLNYNGKTEDHSFDVVDCNLRWMNDRMKVQSPRSFL 300

Query: 301 GKVCKLGETPTLDGMGTIESSPHNNVHNWVGNYPITPNGEDMGAFYAAARDPIFYAHHAN 360
           G+V K GETP    MGTIE SPHNN+H+WVG    TP+ EDMG+FY+AA DP+FYAHH N
Sbjct: 301 GRVLKAGETPKPSDMGTIEQSPHNNIHDWVGTK--TPHFEDMGSFYSAALDPLFYAHHTN 360

Query: 361 VDRVWYIWNKMNSGRIPYIYRQDWLNSTFVFYDEHRNAVRIKVSDCLDSENLGYVYQDVE 420
           +DR+W IW  ++     +    DWLNS+FVFYDE++NAVRI+VSDCLD+E LGYVYQ+V 
Sbjct: 361 IDRLWNIWKTLDGNPKDHT-DPDWLNSSFVFYDENKNAVRIEVSDCLDTEKLGYVYQEVP 420

Query: 421 IPWLNTKQVPRTKR--STGQPEPPVINFPQTLSSAITTSVERPSGVEPQENEEEVLVIED 480
           +PWL   ++P       T +PEPP+INFPQTL+S ITT V+RP      E  + VLV+ED
Sbjct: 421 LPWLLLNKIPSPNPWIRTEEPEPPIINFPQTLNSDITTMVDRPEEAGSGEEVDVVLVLED 480

Query: 481 IEYDPTKNLHFNVLVDVVDEGDDVCPGNVEFLGSFKNIPHGSKHEAKSSLRFVLTEAIEA 540
           +EYDPT  +HF+VLV+VVDE  D C   +EF+GSFKNIPHG  H  K+SLRFV+TE I+ 
Sbjct: 481 VEYDPTTRVHFDVLVNVVDEAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQG 540

Query: 541 LGLHDARKLAVRIDPKLNGDQIKVGGIKIQIEPI 551
           LGL   + LAV+I+P + G+ + +GGIKIQ+EP+
Sbjct: 541 LGLERDKNLAVKIEPMVGGEGVTLGGIKIQLEPV 558

BLAST of MELO3C002580 vs. NCBI nr
Match: KAA0056965.1 (polyphenol oxidase I [Cucumis melo var. makuwa] >TYK26391.1 polyphenol oxidase I [Cucumis melo var. makuwa] >TYK26393.1 polyphenol oxidase I [Cucumis melo var. makuwa])

HSP 1 Score: 589.0 bits (1517), Expect = 4.4e-164
Identity = 323/578 (55.88%), Postives = 395/578 (68.34%), Query Frame = 0

Query: 1   MASLSSLLSLSPYSQCKPQSSIPSKYKALSINMSHAKPQKEKNHQNPQAQGDSQIKFDRR 60
           MASLSSLLS+SPYS CK Q S+    K   +N +H   +   N +      DS  KFDRR
Sbjct: 1   MASLSSLLSVSPYSGCKIQPSVIPLNKRRGVN-NHVSCKASNNGE------DSVNKFDRR 60

Query: 61  DVLFGLGGLYGVTTLGSNKFALAADDDLSLSPDVQNCGPA-DTPVG-PMDCCPPKINKII 120
           D+LFGLGGLYGV TL     ALA  DD SLSPDV NC P   + VG  MDCCPPKI +II
Sbjct: 61  DMLFGLGGLYGVATLSGKDIALA--DDWSLSPDVHNCKPVKPSAVGKKMDCCPPKIKEII 120

Query: 121 DFTKPINPKLHVRPAVQLLTPKQLADFERGIEIMKNLKPDDPRNFYQQAKIHCAYCDSAY 180
           +FT P+ P   VRPAV L+TP+Q+AD E+GI++MK L  DDPR+F  QAK+HCAYCD AY
Sbjct: 121 EFTPPVVPTPRVRPAVHLMTPEQIADLEKGIQMMKMLPEDDPRSFAHQAKVHCAYCDDAY 180

Query: 181 KQIGSEQTDF--------------------LILRHFIGDPDFAIPFWNWDAPIGMHMPIH 240
           KQ+ S   DF                     IL H +  PDFAIPFWNWDAP GM MP  
Sbjct: 181 KQMDSTM-DFQVHENALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMCMPKI 240

Query: 241 YNGNPNSPLYDANRNPLHKAPKVLDLNYDGSTENPPYDVVDCNLRWMNKQMSVKTPTRFL 300
           Y   P+S LYD NR+  H+ PK+L+LNY G     P ++VD NLRWMN QMSV+T   FL
Sbjct: 241 YT-KPSSSLYDENRDKCHQPPKLLNLNYGGKEVKDP-EIVDFNLRWMNDQMSVETAKEFL 300

Query: 301 GKVCKLGETPTLDGMGTIESSPHNNVHNWVGNYPITPNGEDMGAFYAAARDPIFYAHHAN 360
           GKV K G  P  +G G+IE+SPHNN+H WVG+   T +GEDMG FY+A RDP+FYAHHAN
Sbjct: 301 GKVIKRGNGPE-NGSGSIETSPHNNIHIWVGDPEATIHGEDMGHFYSAGRDPLFYAHHAN 360

Query: 361 VDRVWYIWNKMNSGRIPYIYRQDWLNSTFVFYDEHRNAVRIKVSDCLDSENLGYVYQDVE 420
           VDR+W IW KM      +    DWLNS+FV YDE+R AVRIKVS CLDS+ LGYVYQDV 
Sbjct: 361 VDRMWNIWQKMKDRPRDHT-DMDWLNSSFVLYDENRKAVRIKVSQCLDSKILGYVYQDVP 420

Query: 421 IPWLNTKQV--PRTKRSTGQPEPPVINFPQTLSSAITTSVERPSGVEPQE--NEEEVLVI 480
           IPW +T  +  P+ +RS G   PP I+FPQ LSS ITT V+RP    P+E  N+ EVLV+
Sbjct: 421 IPWKDTPPIRRPKQERSLGN-LPPTIDFPQILSSDITTMVDRPEVASPEERINKNEVLVL 480

Query: 481 EDIEYDPTKNLHFNVLVDVVDEGDDVCPGNVEFLGSFKNIPHGSKHEAKSSLRFVLTEAI 540
           ED+EYDPT  LHF+VL++V DEG+DV  G++EF+G+F N+PHG     K+SL FV+T+ I
Sbjct: 481 EDVEYDPTLPLHFDVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRTTYHKTSLHFVITKKI 540

Query: 541 EALGLHDARKLAVRIDPKLN-GDQIKVGGIKIQIEPIE 552
           + LGL   +K+AV+I P +N GD+IK+GGIKIQ+E IE
Sbjct: 541 QQLGLEGDKKVAVKIVPNVNGGDRIKIGGIKIQLEDIE 563

BLAST of MELO3C002580 vs. NCBI nr
Match: KAA0056963.1 (polyphenol oxidase I [Cucumis melo var. makuwa])

HSP 1 Score: 587.0 bits (1512), Expect = 1.7e-163
Identity = 323/578 (55.88%), Postives = 393/578 (67.99%), Query Frame = 0

Query: 1   MASLSSLLSLSPYSQCKPQSSIPSKYKALSINMSHAKPQKEKNHQNPQAQGDSQIKFDRR 60
           MASLSSLLS+SPYS CK Q S+    K   +N +H   +   N +      DS  KFDRR
Sbjct: 1   MASLSSLLSVSPYSGCKIQPSVIPLNKRRGVN-NHVSCKASNNGE------DSVNKFDRR 60

Query: 61  DVLFGLGGLYGVTTLGSNKFALAADDDLSLSPDVQNCGPA-DTPVG-PMDCCPPKINKII 120
           D+LFGLGGLYGV TL     ALA  DD SLSPDV NC P   + VG  MDCCPPKI +II
Sbjct: 61  DMLFGLGGLYGVATLSGKDIALA--DDWSLSPDVHNCKPVKPSAVGKKMDCCPPKIKEII 120

Query: 121 DFTKPINPKLHVRPAVQLLTPKQLADFERGIEIMKNLKPDDPRNFYQQAKIHCAYCDSAY 180
           +FT P+ P   VRPAV L+TP Q+AD E+GI++MK L  DDPR+F  QAK+HCAYCD AY
Sbjct: 121 EFTPPVVPTPRVRPAVHLMTPDQIADLEKGIQMMKMLPEDDPRSFAHQAKVHCAYCDDAY 180

Query: 181 KQIGSEQTDF--------------------LILRHFIGDPDFAIPFWNWDAPIGMHMPIH 240
           KQ+ S   DF                     IL H +  PDFAIPFWNWDAP GM MP  
Sbjct: 181 KQMDSTM-DFQVHENALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMCMPKI 240

Query: 241 YNGNPNSPLYDANRNPLHKAPKVLDLNYDGSTENPPYDVVDCNLRWMNKQMSVKTPTRFL 300
           Y   P+S LYD NR+  H+ PK+L+LNY G       ++VD NLRWMN QMSV+T   FL
Sbjct: 241 YT-KPSSSLYDENRDECHQPPKLLNLNYGGKKVKDS-EIVDFNLRWMNDQMSVETAREFL 300

Query: 301 GKVCKLGETPTLDGMGTIESSPHNNVHNWVGNYPITPNGEDMGAFYAAARDPIFYAHHAN 360
           GKV K G  P  +G G+IE+SPHNN+HNWVG+   T +GEDMG FY+A RDP+FYAHHAN
Sbjct: 301 GKVIKRGNCPE-NGSGSIETSPHNNLHNWVGDPEATIHGEDMGHFYSAGRDPLFYAHHAN 360

Query: 361 VDRVWYIWNKMNSGRIPYIYRQDWLNSTFVFYDEHRNAVRIKVSDCLDSENLGYVYQDVE 420
           VDR+W IW  M   R       DWLNS+FV YDE+R AVRIKVS CLDS+ LGYVYQDV 
Sbjct: 361 VDRMWNIWQNMKDRRDH--TDMDWLNSSFVLYDENRKAVRIKVSQCLDSKILGYVYQDVP 420

Query: 421 IPWLNTKQV--PRTKRSTGQPEPPVINFPQTLSSAITTSVERPSGVEPQE--NEEEVLVI 480
           IPW +T  +  P+ +RS G   PP I+FPQ LSS ITT V+RP    P+E  N+ EVLV+
Sbjct: 421 IPWKDTPPIRRPKQERSLGN-LPPTIDFPQILSSDITTMVDRPEVASPEERINKNEVLVL 480

Query: 481 EDIEYDPTKNLHFNVLVDVVDEGDDVCPGNVEFLGSFKNIPHGSKHEAKSSLRFVLTEAI 540
           ED+EYDPT  LHF+VL++V DEG+DV  G++EF+G+F N+PHG     K+SL FV+T+ I
Sbjct: 481 EDVEYDPTLPLHFDVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRTTYHKTSLHFVITKKI 540

Query: 541 EALGLHDARKLAVRIDPKLN-GDQIKVGGIKIQIEPIE 552
           + LGL   +K+AV+I P +N GD+IK+GGIKIQ+E IE
Sbjct: 541 QQLGLEGDKKVAVKIVPNVNGGDRIKIGGIKIQLEDIE 562

BLAST of MELO3C002580 vs. NCBI nr
Match: XP_004146641.1 (polyphenol oxidase, chloroplastic [Cucumis sativus] >KGN64649.1 hypothetical protein Csa_013698 [Cucumis sativus])

HSP 1 Score: 577.4 bits (1487), Expect = 1.3e-160
Identity = 301/574 (52.44%), Postives = 382/574 (66.55%), Query Frame = 0

Query: 1   MASLSSLLSLSPYSQCKPQSSIPSKYKALSINMSHAKPQKEKNHQNPQAQGDSQIKFDRR 60
           MASLSSLL LS YSQ K +SSI S  + + +N    K              DS  KFDRR
Sbjct: 1   MASLSSLLLLSSYSQTKTKSSISSNKRHVVVNYVSCKAASNNGE-------DSVSKFDRR 60

Query: 61  DVLFGLGGLYGVTTLGSNKFALAADDDLSLSPDVQNCGPADTPVGPMDCCPPKINKIIDF 120
           +VLFGLGGLYGV T  S+  A        L+PD+ NC  A TP G  DCCPP+I KII F
Sbjct: 61  EVLFGLGGLYGVVTATSSVLA------APLTPDIFNCHMAKTPGGIKDCCPPQIGKIIHF 120

Query: 121 TKPINPKLHV-RPAVQLLTPKQLADFERGIEIMKNLKPDDPRNFYQQAKIHCAYCDSAYK 180
             P   + +V RPA  L+   Q+ + E GI+ +K L   DP +F  QAKIHCAYCD  Y 
Sbjct: 121 VPPKIQQPYVRRPAAHLMNQTQIEELESGIKKLKQLDEGDPHSFLHQAKIHCAYCDYVYN 180

Query: 181 QIGS-EQTDF--------------------LILRHFIGDPDFAIPFWNWDAPIGMHMPIH 240
           Q+GS  +T F                     ILRH+IG+P+FAIPFWNWD+P GM+MP  
Sbjct: 181 QLGSITETRFGVHSNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMYMPEF 240

Query: 241 YNGNPNSPLYDANRNPLHKAPKVLDLNYDGSTENPPYDVVDCNLRWMNKQMSVKTPTRFL 300
           YN  P+S LYD  R+P H   K++ LNY+G TE+  +DVVDCNLRWMN +M V++P  FL
Sbjct: 241 YN-RPSSSLYDHLRDPRHDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRMQVQSPRSFL 300

Query: 301 GKVCKLGETPTLDGMGTIESSPHNNVHNWVGNYPITPNGEDMGAFYAAARDPIFYAHHAN 360
           G+V K GETP    MGTIE SPHNN+H+W+G    TP+ EDMG+FY+AA DP+FYAHHAN
Sbjct: 301 GRVLKAGETPNPTDMGTIEQSPHNNIHDWLGTK--TPHFEDMGSFYSAALDPLFYAHHAN 360

Query: 361 VDRVWYIWNKMNSGRIPYIYRQDWLNSTFVFYDEHRNAVRIKVSDCLDSENLGYVYQDVE 420
           +DR+W IW  ++     +I   DWLNS+FVFYDE++NAV IKVSDCLD+E LGYVYQ+V 
Sbjct: 361 IDRLWNIWKTLDGNPKDHI-DPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEVP 420

Query: 421 IPWLNTKQVPRTKR--STGQPEPPVINFPQTLSSAITTSVERPSGVEPQENEEEVLVIED 480
           +PWL+  ++P       T + EPP++NFPQ L+S ITT V+RP      E  +EVLV+ED
Sbjct: 421 LPWLHMNKIPTPNPWIRTEESEPPIVNFPQILNSDITTMVDRPEEDRSGEEVDEVLVLED 480

Query: 481 IEYDPTKNLHFNVLVDVVDEGDDVCPGNVEFLGSFKNIPHGSKHEAKSSLRFVLTEAIEA 540
           +EYDPT  +HF+VLV+VVD+  D C   +EF+GSFKNIPHG  H  K+SLRFV+TE I+ 
Sbjct: 481 VEYDPTTRVHFDVLVNVVDKAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQG 540

Query: 541 LGLHDARKLAVRIDPKLNGDQIKVGGIKIQIEPI 551
           LGL   + LAV+I+P + G+ + VGGIKIQ+EP+
Sbjct: 541 LGLERDKNLAVKIEPMVGGEGVTVGGIKIQLEPL 557

BLAST of MELO3C002580 vs. ExPASy TrEMBL
Match: A0A5A7UPD3 (Polyphenol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00590 PE=3 SV=1)

HSP 1 Score: 1149.8 bits (2973), Expect = 0.0e+00
Identity = 551/551 (100.00%), Postives = 551/551 (100.00%), Query Frame = 0

Query: 1   MASLSSLLSLSPYSQCKPQSSIPSKYKALSINMSHAKPQKEKNHQNPQAQGDSQIKFDRR 60
           MASLSSLLSLSPYSQCKPQSSIPSKYKALSINMSHAKPQKEKNHQNPQAQGDSQIKFDRR
Sbjct: 1   MASLSSLLSLSPYSQCKPQSSIPSKYKALSINMSHAKPQKEKNHQNPQAQGDSQIKFDRR 60

Query: 61  DVLFGLGGLYGVTTLGSNKFALAADDDLSLSPDVQNCGPADTPVGPMDCCPPKINKIIDF 120
           DVLFGLGGLYGVTTLGSNKFALAADDDLSLSPDVQNCGPADTPVGPMDCCPPKINKIIDF
Sbjct: 61  DVLFGLGGLYGVTTLGSNKFALAADDDLSLSPDVQNCGPADTPVGPMDCCPPKINKIIDF 120

Query: 121 TKPINPKLHVRPAVQLLTPKQLADFERGIEIMKNLKPDDPRNFYQQAKIHCAYCDSAYKQ 180
           TKPINPKLHVRPAVQLLTPKQLADFERGIEIMKNLKPDDPRNFYQQAKIHCAYCDSAYKQ
Sbjct: 121 TKPINPKLHVRPAVQLLTPKQLADFERGIEIMKNLKPDDPRNFYQQAKIHCAYCDSAYKQ 180

Query: 181 IGSEQTDFLILRHFIGDPDFAIPFWNWDAPIGMHMPIHYNGNPNSPLYDANRNPLHKAPK 240
           IGSEQTDFLILRHFIGDPDFAIPFWNWDAPIGMHMPIHYNGNPNSPLYDANRNPLHKAPK
Sbjct: 181 IGSEQTDFLILRHFIGDPDFAIPFWNWDAPIGMHMPIHYNGNPNSPLYDANRNPLHKAPK 240

Query: 241 VLDLNYDGSTENPPYDVVDCNLRWMNKQMSVKTPTRFLGKVCKLGETPTLDGMGTIESSP 300
           VLDLNYDGSTENPPYDVVDCNLRWMNKQMSVKTPTRFLGKVCKLGETPTLDGMGTIESSP
Sbjct: 241 VLDLNYDGSTENPPYDVVDCNLRWMNKQMSVKTPTRFLGKVCKLGETPTLDGMGTIESSP 300

Query: 301 HNNVHNWVGNYPITPNGEDMGAFYAAARDPIFYAHHANVDRVWYIWNKMNSGRIPYIYRQ 360
           HNNVHNWVGNYPITPNGEDMGAFYAAARDPIFYAHHANVDRVWYIWNKMNSGRIPYIYRQ
Sbjct: 301 HNNVHNWVGNYPITPNGEDMGAFYAAARDPIFYAHHANVDRVWYIWNKMNSGRIPYIYRQ 360

Query: 361 DWLNSTFVFYDEHRNAVRIKVSDCLDSENLGYVYQDVEIPWLNTKQVPRTKRSTGQPEPP 420
           DWLNSTFVFYDEHRNAVRIKVSDCLDSENLGYVYQDVEIPWLNTKQVPRTKRSTGQPEPP
Sbjct: 361 DWLNSTFVFYDEHRNAVRIKVSDCLDSENLGYVYQDVEIPWLNTKQVPRTKRSTGQPEPP 420

Query: 421 VINFPQTLSSAITTSVERPSGVEPQENEEEVLVIEDIEYDPTKNLHFNVLVDVVDEGDDV 480
           VINFPQTLSSAITTSVERPSGVEPQENEEEVLVIEDIEYDPTKNLHFNVLVDVVDEGDDV
Sbjct: 421 VINFPQTLSSAITTSVERPSGVEPQENEEEVLVIEDIEYDPTKNLHFNVLVDVVDEGDDV 480

Query: 481 CPGNVEFLGSFKNIPHGSKHEAKSSLRFVLTEAIEALGLHDARKLAVRIDPKLNGDQIKV 540
           CPGNVEFLGSFKNIPHGSKHEAKSSLRFVLTEAIEALGLHDARKLAVRIDPKLNGDQIKV
Sbjct: 481 CPGNVEFLGSFKNIPHGSKHEAKSSLRFVLTEAIEALGLHDARKLAVRIDPKLNGDQIKV 540

Query: 541 GGIKIQIEPIE 552
           GGIKIQIEPIE
Sbjct: 541 GGIKIQIEPIE 551

BLAST of MELO3C002580 vs. ExPASy TrEMBL
Match: A0A1S3BFK3 (polyphenol oxidase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103489307 PE=3 SV=1)

HSP 1 Score: 1149.8 bits (2973), Expect = 0.0e+00
Identity = 551/551 (100.00%), Postives = 551/551 (100.00%), Query Frame = 0

Query: 1   MASLSSLLSLSPYSQCKPQSSIPSKYKALSINMSHAKPQKEKNHQNPQAQGDSQIKFDRR 60
           MASLSSLLSLSPYSQCKPQSSIPSKYKALSINMSHAKPQKEKNHQNPQAQGDSQIKFDRR
Sbjct: 1   MASLSSLLSLSPYSQCKPQSSIPSKYKALSINMSHAKPQKEKNHQNPQAQGDSQIKFDRR 60

Query: 61  DVLFGLGGLYGVTTLGSNKFALAADDDLSLSPDVQNCGPADTPVGPMDCCPPKINKIIDF 120
           DVLFGLGGLYGVTTLGSNKFALAADDDLSLSPDVQNCGPADTPVGPMDCCPPKINKIIDF
Sbjct: 61  DVLFGLGGLYGVTTLGSNKFALAADDDLSLSPDVQNCGPADTPVGPMDCCPPKINKIIDF 120

Query: 121 TKPINPKLHVRPAVQLLTPKQLADFERGIEIMKNLKPDDPRNFYQQAKIHCAYCDSAYKQ 180
           TKPINPKLHVRPAVQLLTPKQLADFERGIEIMKNLKPDDPRNFYQQAKIHCAYCDSAYKQ
Sbjct: 121 TKPINPKLHVRPAVQLLTPKQLADFERGIEIMKNLKPDDPRNFYQQAKIHCAYCDSAYKQ 180

Query: 181 IGSEQTDFLILRHFIGDPDFAIPFWNWDAPIGMHMPIHYNGNPNSPLYDANRNPLHKAPK 240
           IGSEQTDFLILRHFIGDPDFAIPFWNWDAPIGMHMPIHYNGNPNSPLYDANRNPLHKAPK
Sbjct: 181 IGSEQTDFLILRHFIGDPDFAIPFWNWDAPIGMHMPIHYNGNPNSPLYDANRNPLHKAPK 240

Query: 241 VLDLNYDGSTENPPYDVVDCNLRWMNKQMSVKTPTRFLGKVCKLGETPTLDGMGTIESSP 300
           VLDLNYDGSTENPPYDVVDCNLRWMNKQMSVKTPTRFLGKVCKLGETPTLDGMGTIESSP
Sbjct: 241 VLDLNYDGSTENPPYDVVDCNLRWMNKQMSVKTPTRFLGKVCKLGETPTLDGMGTIESSP 300

Query: 301 HNNVHNWVGNYPITPNGEDMGAFYAAARDPIFYAHHANVDRVWYIWNKMNSGRIPYIYRQ 360
           HNNVHNWVGNYPITPNGEDMGAFYAAARDPIFYAHHANVDRVWYIWNKMNSGRIPYIYRQ
Sbjct: 301 HNNVHNWVGNYPITPNGEDMGAFYAAARDPIFYAHHANVDRVWYIWNKMNSGRIPYIYRQ 360

Query: 361 DWLNSTFVFYDEHRNAVRIKVSDCLDSENLGYVYQDVEIPWLNTKQVPRTKRSTGQPEPP 420
           DWLNSTFVFYDEHRNAVRIKVSDCLDSENLGYVYQDVEIPWLNTKQVPRTKRSTGQPEPP
Sbjct: 361 DWLNSTFVFYDEHRNAVRIKVSDCLDSENLGYVYQDVEIPWLNTKQVPRTKRSTGQPEPP 420

Query: 421 VINFPQTLSSAITTSVERPSGVEPQENEEEVLVIEDIEYDPTKNLHFNVLVDVVDEGDDV 480
           VINFPQTLSSAITTSVERPSGVEPQENEEEVLVIEDIEYDPTKNLHFNVLVDVVDEGDDV
Sbjct: 421 VINFPQTLSSAITTSVERPSGVEPQENEEEVLVIEDIEYDPTKNLHFNVLVDVVDEGDDV 480

Query: 481 CPGNVEFLGSFKNIPHGSKHEAKSSLRFVLTEAIEALGLHDARKLAVRIDPKLNGDQIKV 540
           CPGNVEFLGSFKNIPHGSKHEAKSSLRFVLTEAIEALGLHDARKLAVRIDPKLNGDQIKV
Sbjct: 481 CPGNVEFLGSFKNIPHGSKHEAKSSLRFVLTEAIEALGLHDARKLAVRIDPKLNGDQIKV 540

Query: 541 GGIKIQIEPIE 552
           GGIKIQIEPIE
Sbjct: 541 GGIKIQIEPIE 551

BLAST of MELO3C002580 vs. ExPASy TrEMBL
Match: A0A5A7UM88 (Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00520 PE=3 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 8.6e-166
Identity = 308/574 (53.66%), Postives = 386/574 (67.25%), Query Frame = 0

Query: 1   MASLSSLLSLSPYSQCKPQSSIPSKYKALSINMSHAKPQKEKNHQNPQAQGDSQIKFDRR 60
           MASLSSLL LSPYSQ K + SI S  + + +N    K     N +      DS  KFDRR
Sbjct: 1   MASLSSLLLLSPYSQTKTKFSISSNKRHVVVNYVSCKAASSNNGE------DSVSKFDRR 60

Query: 61  DVLFGLGGLYGVTTLGSNKFALAADDDLSLSPDVQNCGPADTPVGPMDCCPPKINKIIDF 120
           +VLFGLGGLYGV T   +  A        L+PDV NC  A TP G  +CCPP+I KII F
Sbjct: 61  EVLFGLGGLYGVVTATGSVLA------APLTPDVFNCDMAQTPGGIKNCCPPRIGKIIHF 120

Query: 121 TKPINPKLHV-RPAVQLLTPKQLADFERGIEIMKNLKPDDPRNFYQQAKIHCAYCDSAYK 180
             P   + +V RPA  L+   Q+ + ERGI+ +K L  DDP +F  QAKIHCAYCD AY 
Sbjct: 121 VPPKIQQPYVRRPAAHLMNQTQIEELERGIQKLKQLDEDDPHSFSHQAKIHCAYCDYAYN 180

Query: 181 QIGS-EQTDF--------------------LILRHFIGDPDFAIPFWNWDAPIGMHMPIH 240
           Q+GS  +T F                     ILRH+IG+PDFAIPFWNWD+P GMHMP  
Sbjct: 181 QLGSITETRFEVHSNALFFPFHRAYLYFFERILRHYIGNPDFAIPFWNWDSPPGMHMPEF 240

Query: 241 YNGNPNSPLYDANRNPLHKAPKVLDLNYDGSTENPPYDVVDCNLRWMNKQMSVKTPTRFL 300
           YN  P+S LYD  R+P H   K++DLNY+G TE+  +DVVDCNLRWMN +M V++P  FL
Sbjct: 241 YN-RPSSSLYDNLRDPRHDPHKLIDLNYNGKTEDHSFDVVDCNLRWMNDRMKVQSPRSFL 300

Query: 301 GKVCKLGETPTLDGMGTIESSPHNNVHNWVGNYPITPNGEDMGAFYAAARDPIFYAHHAN 360
           G+V K GETP    MGTIE SPHNN+H+WVG    TP+ EDMG+FY+AA DP+FYAHH N
Sbjct: 301 GRVLKAGETPKPSDMGTIEQSPHNNIHDWVGTK--TPHFEDMGSFYSAALDPLFYAHHTN 360

Query: 361 VDRVWYIWNKMNSGRIPYIYRQDWLNSTFVFYDEHRNAVRIKVSDCLDSENLGYVYQDVE 420
           +DR+W IW  ++     +    DWLNS+FVFYDE++NAVRI+VSDCLD+E LGYVYQ+V 
Sbjct: 361 IDRLWNIWKTLDGNPKDHT-DPDWLNSSFVFYDENKNAVRIEVSDCLDTEKLGYVYQEVP 420

Query: 421 IPWLNTKQVPRTKR--STGQPEPPVINFPQTLSSAITTSVERPSGVEPQENEEEVLVIED 480
           +PWL   ++P       T +PEPP+INFPQTL+S ITT V+RP      E  + VLV+ED
Sbjct: 421 LPWLLLNKIPSPNPWIRTEEPEPPIINFPQTLNSDITTMVDRPEEAGSGEEVDVVLVLED 480

Query: 481 IEYDPTKNLHFNVLVDVVDEGDDVCPGNVEFLGSFKNIPHGSKHEAKSSLRFVLTEAIEA 540
           +EYDPT  +HF+VLV+VVDE  D C   +EF+GSFKNIPHG  H  K+SLRFV+TE I+ 
Sbjct: 481 VEYDPTTRVHFDVLVNVVDEAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQG 540

Query: 541 LGLHDARKLAVRIDPKLNGDQIKVGGIKIQIEPI 551
           LGL   + LAV+I+P + G+ + +GGIKIQ+EP+
Sbjct: 541 LGLERDKNLAVKIEPMVGGEGVTLGGIKIQLEPV 558

BLAST of MELO3C002580 vs. ExPASy TrEMBL
Match: A0A5A7UTU0 (Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00530 PE=3 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 2.1e-164
Identity = 323/578 (55.88%), Postives = 395/578 (68.34%), Query Frame = 0

Query: 1   MASLSSLLSLSPYSQCKPQSSIPSKYKALSINMSHAKPQKEKNHQNPQAQGDSQIKFDRR 60
           MASLSSLLS+SPYS CK Q S+    K   +N +H   +   N +      DS  KFDRR
Sbjct: 1   MASLSSLLSVSPYSGCKIQPSVIPLNKRRGVN-NHVSCKASNNGE------DSVNKFDRR 60

Query: 61  DVLFGLGGLYGVTTLGSNKFALAADDDLSLSPDVQNCGPA-DTPVG-PMDCCPPKINKII 120
           D+LFGLGGLYGV TL     ALA  DD SLSPDV NC P   + VG  MDCCPPKI +II
Sbjct: 61  DMLFGLGGLYGVATLSGKDIALA--DDWSLSPDVHNCKPVKPSAVGKKMDCCPPKIKEII 120

Query: 121 DFTKPINPKLHVRPAVQLLTPKQLADFERGIEIMKNLKPDDPRNFYQQAKIHCAYCDSAY 180
           +FT P+ P   VRPAV L+TP+Q+AD E+GI++MK L  DDPR+F  QAK+HCAYCD AY
Sbjct: 121 EFTPPVVPTPRVRPAVHLMTPEQIADLEKGIQMMKMLPEDDPRSFAHQAKVHCAYCDDAY 180

Query: 181 KQIGSEQTDF--------------------LILRHFIGDPDFAIPFWNWDAPIGMHMPIH 240
           KQ+ S   DF                     IL H +  PDFAIPFWNWDAP GM MP  
Sbjct: 181 KQMDSTM-DFQVHENALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMCMPKI 240

Query: 241 YNGNPNSPLYDANRNPLHKAPKVLDLNYDGSTENPPYDVVDCNLRWMNKQMSVKTPTRFL 300
           Y   P+S LYD NR+  H+ PK+L+LNY G     P ++VD NLRWMN QMSV+T   FL
Sbjct: 241 YT-KPSSSLYDENRDKCHQPPKLLNLNYGGKEVKDP-EIVDFNLRWMNDQMSVETAKEFL 300

Query: 301 GKVCKLGETPTLDGMGTIESSPHNNVHNWVGNYPITPNGEDMGAFYAAARDPIFYAHHAN 360
           GKV K G  P  +G G+IE+SPHNN+H WVG+   T +GEDMG FY+A RDP+FYAHHAN
Sbjct: 301 GKVIKRGNGPE-NGSGSIETSPHNNIHIWVGDPEATIHGEDMGHFYSAGRDPLFYAHHAN 360

Query: 361 VDRVWYIWNKMNSGRIPYIYRQDWLNSTFVFYDEHRNAVRIKVSDCLDSENLGYVYQDVE 420
           VDR+W IW KM      +    DWLNS+FV YDE+R AVRIKVS CLDS+ LGYVYQDV 
Sbjct: 361 VDRMWNIWQKMKDRPRDHT-DMDWLNSSFVLYDENRKAVRIKVSQCLDSKILGYVYQDVP 420

Query: 421 IPWLNTKQV--PRTKRSTGQPEPPVINFPQTLSSAITTSVERPSGVEPQE--NEEEVLVI 480
           IPW +T  +  P+ +RS G   PP I+FPQ LSS ITT V+RP    P+E  N+ EVLV+
Sbjct: 421 IPWKDTPPIRRPKQERSLGN-LPPTIDFPQILSSDITTMVDRPEVASPEERINKNEVLVL 480

Query: 481 EDIEYDPTKNLHFNVLVDVVDEGDDVCPGNVEFLGSFKNIPHGSKHEAKSSLRFVLTEAI 540
           ED+EYDPT  LHF+VL++V DEG+DV  G++EF+G+F N+PHG     K+SL FV+T+ I
Sbjct: 481 EDVEYDPTLPLHFDVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRTTYHKTSLHFVITKKI 540

Query: 541 EALGLHDARKLAVRIDPKLN-GDQIKVGGIKIQIEPIE 552
           + LGL   +K+AV+I P +N GD+IK+GGIKIQ+E IE
Sbjct: 541 QQLGLEGDKKVAVKIVPNVNGGDRIKIGGIKIQLEDIE 563

BLAST of MELO3C002580 vs. ExPASy TrEMBL
Match: A0A5A7UM24 (Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G001410 PE=3 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 8.0e-164
Identity = 323/578 (55.88%), Postives = 393/578 (67.99%), Query Frame = 0

Query: 1   MASLSSLLSLSPYSQCKPQSSIPSKYKALSINMSHAKPQKEKNHQNPQAQGDSQIKFDRR 60
           MASLSSLLS+SPYS CK Q S+    K   +N +H   +   N +      DS  KFDRR
Sbjct: 1   MASLSSLLSVSPYSGCKIQPSVIPLNKRRGVN-NHVSCKASNNGE------DSVNKFDRR 60

Query: 61  DVLFGLGGLYGVTTLGSNKFALAADDDLSLSPDVQNCGPA-DTPVG-PMDCCPPKINKII 120
           D+LFGLGGLYGV TL     ALA  DD SLSPDV NC P   + VG  MDCCPPKI +II
Sbjct: 61  DMLFGLGGLYGVATLSGKDIALA--DDWSLSPDVHNCKPVKPSAVGKKMDCCPPKIKEII 120

Query: 121 DFTKPINPKLHVRPAVQLLTPKQLADFERGIEIMKNLKPDDPRNFYQQAKIHCAYCDSAY 180
           +FT P+ P   VRPAV L+TP Q+AD E+GI++MK L  DDPR+F  QAK+HCAYCD AY
Sbjct: 121 EFTPPVVPTPRVRPAVHLMTPDQIADLEKGIQMMKMLPEDDPRSFAHQAKVHCAYCDDAY 180

Query: 181 KQIGSEQTDF--------------------LILRHFIGDPDFAIPFWNWDAPIGMHMPIH 240
           KQ+ S   DF                     IL H +  PDFAIPFWNWDAP GM MP  
Sbjct: 181 KQMDSTM-DFQVHENALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMCMPKI 240

Query: 241 YNGNPNSPLYDANRNPLHKAPKVLDLNYDGSTENPPYDVVDCNLRWMNKQMSVKTPTRFL 300
           Y   P+S LYD NR+  H+ PK+L+LNY G       ++VD NLRWMN QMSV+T   FL
Sbjct: 241 YT-KPSSSLYDENRDECHQPPKLLNLNYGGKKVKDS-EIVDFNLRWMNDQMSVETAREFL 300

Query: 301 GKVCKLGETPTLDGMGTIESSPHNNVHNWVGNYPITPNGEDMGAFYAAARDPIFYAHHAN 360
           GKV K G  P  +G G+IE+SPHNN+HNWVG+   T +GEDMG FY+A RDP+FYAHHAN
Sbjct: 301 GKVIKRGNCPE-NGSGSIETSPHNNLHNWVGDPEATIHGEDMGHFYSAGRDPLFYAHHAN 360

Query: 361 VDRVWYIWNKMNSGRIPYIYRQDWLNSTFVFYDEHRNAVRIKVSDCLDSENLGYVYQDVE 420
           VDR+W IW  M   R       DWLNS+FV YDE+R AVRIKVS CLDS+ LGYVYQDV 
Sbjct: 361 VDRMWNIWQNMKDRRDH--TDMDWLNSSFVLYDENRKAVRIKVSQCLDSKILGYVYQDVP 420

Query: 421 IPWLNTKQV--PRTKRSTGQPEPPVINFPQTLSSAITTSVERPSGVEPQE--NEEEVLVI 480
           IPW +T  +  P+ +RS G   PP I+FPQ LSS ITT V+RP    P+E  N+ EVLV+
Sbjct: 421 IPWKDTPPIRRPKQERSLGN-LPPTIDFPQILSSDITTMVDRPEVASPEERINKNEVLVL 480

Query: 481 EDIEYDPTKNLHFNVLVDVVDEGDDVCPGNVEFLGSFKNIPHGSKHEAKSSLRFVLTEAI 540
           ED+EYDPT  LHF+VL++V DEG+DV  G++EF+G+F N+PHG     K+SL FV+T+ I
Sbjct: 481 EDVEYDPTLPLHFDVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRTTYHKTSLHFVITKKI 540

Query: 541 EALGLHDARKLAVRIDPKLN-GDQIKVGGIKIQIEPIE 552
           + LGL   +K+AV+I P +N GD+IK+GGIKIQ+E IE
Sbjct: 541 QQLGLEGDKKVAVKIVPNVNGGDRIKIGGIKIQLEDIE 562

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P433092.6e-11141.54Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1[more]
Q6UIL33.4e-10340.37(+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata OX=66636 PE=1 S... [more]
P433111.9e-10140.03Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1[more]
Q9MB142.1e-10041.34Polyphenol oxidase II, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-2 PE=2 SV=... [more]
O811031.1e-9838.28Polyphenol oxidase latent form, chloroplastic OS=Prunus armeniaca OX=36596 GN=PP... [more]
Match NameE-valueIdentityDescription
XP_008446638.10.0e+00100.00PREDICTED: polyphenol oxidase, chloroplastic-like [Cucumis melo] >KAA0056970.1 p... [more]
KAA0056962.11.8e-16553.66polyphenol oxidase I [Cucumis melo var. makuwa] >TYK26390.1 polyphenol oxidase I... [more]
KAA0056965.14.4e-16455.88polyphenol oxidase I [Cucumis melo var. makuwa] >TYK26391.1 polyphenol oxidase I... [more]
KAA0056963.11.7e-16355.88polyphenol oxidase I [Cucumis melo var. makuwa][more]
XP_004146641.11.3e-16052.44polyphenol oxidase, chloroplastic [Cucumis sativus] >KGN64649.1 hypothetical pro... [more]
Match NameE-valueIdentityDescription
A0A5A7UPD30.0e+00100.00Polyphenol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G0... [more]
A0A1S3BFK30.0e+00100.00polyphenol oxidase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103489307 P... [more]
A0A5A7UM888.6e-16653.66Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861... [more]
A0A5A7UTU02.1e-16455.88Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861... [more]
A0A5A7UM248.0e-16455.88Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002227Tyrosinase copper-binding domainPRINTSPR00092TYROSINASEcoord: 202..207
score: 57.82
coord: 300..311
score: 41.5
coord: 328..346
score: 57.25
IPR002227Tyrosinase copper-binding domainPFAMPF00264Tyrosinasecoord: 191..348
e-value: 2.6E-22
score: 80.2
IPR002227Tyrosinase copper-binding domainPROSITEPS00498TYROSINASE_2coord: 329..340
IPR022740Polyphenol oxidase, C-terminalPFAMPF12143PPO1_KFDVcoord: 424..546
e-value: 9.4E-28
score: 96.9
IPR022739Polyphenol oxidase, central domainPFAMPF12142PPO1_DWLcoord: 360..405
e-value: 3.9E-22
score: 77.8
IPR008922Uncharacterised domain, di-copper centreGENE3D1.10.1280.10coord: 89..406
e-value: 4.5E-81
score: 275.0
IPR008922Uncharacterised domain, di-copper centreSUPERFAMILY48056Di-copper centre-containing domaincoord: 103..402
NoneNo IPR availablePIRSRPIRSR000290-1PIRSR000290-1coord: 190..547
e-value: 3.5E-111
score: 370.4
NoneNo IPR availablePANTHERPTHR11474TYROSINASE FAMILY MEMBERcoord: 47..547
NoneNo IPR availablePANTHERPTHR11474:SF115OS04G0624500 PROTEINcoord: 47..547

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C002580.1MELO3C002580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004097 catechol oxidase activity
molecular_function GO:0016491 oxidoreductase activity