MELO.jh101542.1 (gene) Melon (Harukei-3) v1.41

Overview
NameMELO.jh101542.1
Typegene
OrganismCucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
Descriptiondisease resistance protein RGA2-like
Locationchr04: 29057300 .. 29058352 (+)
RNA-Seq ExpressionMELO.jh101542.1
SyntenyMELO.jh101542.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGAATTTCTATGGACTTTTGCTGTTCAGGAAGTTTTGAAGAAGATTGTGAAATATGGAGCAGAGCAAATTGTTGTGGCATGGGAGTTGGAGGATGAGGTGTCCCTGTTGAAAGACAAGTTGCACGATGCTGATGCAATCTTAGAAGACATCAACAGAAAGAAATCACACCCTGGTAATTCTGTGAAGAGATGGGTGGAAAAACTTGAAGATATTGTCCATGAAGCCGATGATCTACTGGATGAGCTTGTTTATGAACATCTTCGACGAACTGTGGAGCATACTGACAAATTTAGGAAGGTACGTGATTCAATCTCACCATCCAAAAATTCTTTTTTGTTTCGTCGCAAGATGGCCAAGAAAATCAAGAACATTACTGATACTTTATACAAACATTATTGTGCGGCAAGTGCTTTTGGGCTCGTTGGTGTGGAATCTGTCACAGAAACAGAGCTTGCTCTCAGACAGATACGAGAGACAACCTCAATTCTTGACTTCCAAGTTGAAGGAAGGGAAGCTGAAGCTTTGGAGCTCATTAAATTGGCGATCGACTCTGCCAATGAACATCATCTGTCTGTGATATCCATTGTTGGAATGGGTGGGCTTGGGAAAACGACTTTGGCCAAGATGATCTTCAATCACGACGCCATTAAAAGACATTTTGATCAAACTATATGGGTCTGCGTGTCTAAACCATTTACTGTTACGAAAATTTTGGAACAAATTTTTCAAGGTTTAACGAATAATTGTAGTGGGTTGGAATCCAATAAGGAGGCGTTGCTTGGGAGGCTTCGAAAGGAGATGCAAGACAAGAAGTATTTTCTTGTGCTTGATGATGTTTGGGATGATGAGAAACGCTTGTGGGACGAACTTTGTGGCTGTTTGAAACATATCGCTGGAAAACATGGAAATAGTATAATGGTGACAACAAGAGATGTAAAAGTGGCAACCATGGTGGAGCCAATTTCCATTCATCATCTAACAAAATTATCTGATGATCAATGTTGGGCATTGTTTAAAGAAAGTCAAATGCAAATCAGCTACCAATGA

mRNA sequence

ATGGCTGAATTTCTATGGACTTTTGCTGTTCAGGAAGTTTTGAAGAAGATTGTGAAATATGGAGCAGAGCAAATTGTTGTGGCATGGGAGTTGGAGGATGAGGTGTCCCTGTTGAAAGACAAGTTGCACGATGCTGATGCAATCTTAGAAGACATCAACAGAAAGAAATCACACCCTGGTAATTCTGTGAAGAGATGGGTGGAAAAACTTGAAGATATTGTCCATGAAGCCGATGATCTACTGGATGAGCTTGTTTATGAACATCTTCGACGAACTGTGGAGCATACTGACAAATTTAGGAAGGTACGTGATTCAATCTCACCATCCAAAAATTCTTTTTTGTTTCGTCGCAAGATGGCCAAGAAAATCAAGAACATTACTGATACTTTATACAAACATTATTGTGCGGCAAGTGCTTTTGGGCTCGTTGGTGTGGAATCTGTCACAGAAACAGAGCTTGCTCTCAGACAGATACGAGAGACAACCTCAATTCTTGACTTCCAAGTTGAAGGAAGGGAAGCTGAAGCTTTGGAGCTCATTAAATTGGCGATCGACTCTGCCAATGAACATCATCTGTCTGTGATATCCATTGTTGGAATGGGTGGGCTTGGGAAAACGACTTTGGCCAAGATGATCTTCAATCACGACGCCATTAAAAGACATTTTGATCAAACTATATGGGTCTGCGTGTCTAAACCATTTACTGTTACGAAAATTTTGGAACAAATTTTTCAAGGTTTAACGAATAATTGTAGTGGGTTGGAATCCAATAAGGAGGCGTTGCTTGGGAGGCTTCGAAAGGAGATGCAAGACAAGAAGTATTTTCTTGTGCTTGATGATGTTTGGGATGATGAGAAACGCTTGTGGGACGAACTTTGTGGCTGTTTGAAACATATCGCTGGAAAACATGGAAATAGTATAATGGTGACAACAAGAGATGTAAAAGTGGCAACCATGGTGGAGCCAATTTCCATTCATCATCTAACAAAATTATCTGATGATCAATGTTGGGCATTGTTTAAAGAAAGTCAAATGCAAATCAGCTACCAATGA

Coding sequence (CDS)

ATGGCTGAATTTCTATGGACTTTTGCTGTTCAGGAAGTTTTGAAGAAGATTGTGAAATATGGAGCAGAGCAAATTGTTGTGGCATGGGAGTTGGAGGATGAGGTGTCCCTGTTGAAAGACAAGTTGCACGATGCTGATGCAATCTTAGAAGACATCAACAGAAAGAAATCACACCCTGGTAATTCTGTGAAGAGATGGGTGGAAAAACTTGAAGATATTGTCCATGAAGCCGATGATCTACTGGATGAGCTTGTTTATGAACATCTTCGACGAACTGTGGAGCATACTGACAAATTTAGGAAGGTACGTGATTCAATCTCACCATCCAAAAATTCTTTTTTGTTTCGTCGCAAGATGGCCAAGAAAATCAAGAACATTACTGATACTTTATACAAACATTATTGTGCGGCAAGTGCTTTTGGGCTCGTTGGTGTGGAATCTGTCACAGAAACAGAGCTTGCTCTCAGACAGATACGAGAGACAACCTCAATTCTTGACTTCCAAGTTGAAGGAAGGGAAGCTGAAGCTTTGGAGCTCATTAAATTGGCGATCGACTCTGCCAATGAACATCATCTGTCTGTGATATCCATTGTTGGAATGGGTGGGCTTGGGAAAACGACTTTGGCCAAGATGATCTTCAATCACGACGCCATTAAAAGACATTTTGATCAAACTATATGGGTCTGCGTGTCTAAACCATTTACTGTTACGAAAATTTTGGAACAAATTTTTCAAGGTTTAACGAATAATTGTAGTGGGTTGGAATCCAATAAGGAGGCGTTGCTTGGGAGGCTTCGAAAGGAGATGCAAGACAAGAAGTATTTTCTTGTGCTTGATGATGTTTGGGATGATGAGAAACGCTTGTGGGACGAACTTTGTGGCTGTTTGAAACATATCGCTGGAAAACATGGAAATAGTATAATGGTGACAACAAGAGATGTAAAAGTGGCAACCATGGTGGAGCCAATTTCCATTCATCATCTAACAAAATTATCTGATGATCAATGTTGGGCATTGTTTAAAGAAAGTCAAATGCAAATCAGCTACCAATGA

Protein sequence

MAEFLWTFAVQEVLKKIVKYGAEQIVVAWELEDEVSLLKDKLHDADAILEDINRKKSHPGNSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTDKFRKVRDSISPSKNSFLFRRKMAKKIKNITDTLYKHYCAASAFGLVGVESVTETELALRQIRETTSILDFQVEGREAEALELIKLAIDSANEHHLSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFTVTKILEQIFQGLTNNCSGLESNKEALLGRLRKEMQDKKYFLVLDDVWDDEKRLWDELCGCLKHIAGKHGNSIMVTTRDVKVATMVEPISIHHLTKLSDDQCWALFKESQMQISYQ
Homology
BLAST of MELO.jh101542.1 vs. NCBI nr
Match: XP_004136783.2 (disease resistance protein RGA2 [Cucumis sativus])

HSP 1 Score: 588 bits (1517), Expect = 3.69e-200
Identity = 300/343 (87.46%), Postives = 317/343 (92.42%), Query Frame = 0

Query: 1   MAEFLWTFAVQEVLKKIVKYGAEQIVVAWELEDEVSLLKDKLHDADAILEDINRKKSHPG 60
           MAE + TF VQEVLK+IVKYGAEQIVVAWELE+EVSLLKDKLHDAD ILEDINRKKSHPG
Sbjct: 1   MAE-IGTFVVQEVLKRIVKYGAEQIVVAWELENEVSLLKDKLHDADTILEDINRKKSHPG 60

Query: 61  NSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTDKFRKVRDSISPSKNSFLFRRKMA 120
           NSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHT+KF KV DSIS S NSFLFRRKMA
Sbjct: 61  NSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTEKFSKVSDSISSSINSFLFRRKMA 120

Query: 121 KKIKNITDTLYKHYCAASAFGLVGVESVTETELALRQIRETTSILDFQVEGREAEALELI 180
           KKIKNITDTL +HYCAASAFGLVGVE+VTE ELAL QIRETTSILDFQVEGREAE LEL+
Sbjct: 121 KKIKNITDTLNQHYCAASAFGLVGVETVTEIELALNQIRETTSILDFQVEGREAEVLELL 180

Query: 181 KLAIDSANEHHLSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFTVTKIL 240
           KLAIDS NEHH+SVISIVGMGGLGKTTLAKMIFNH  I+ HFD+TIWVCVSKPF VTKIL
Sbjct: 181 KLAIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVTKIL 240

Query: 241 EQIFQGLTNNCSGLESNKEALLGRLRKEMQDKKYFLVLDDVWDDEKRLWDELCGCLKHIA 300
           E+IFQGLT  CSGLESNKEALLGRLRKEMQDK YFLVLDDVWD+EK LWDEL GCLKHIA
Sbjct: 241 EKIFQGLTKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRGCLKHIA 300

Query: 301 GKHGNSIMVTTRDVKVATMVEPISIHHLTKLSDDQCWALFKES 343
           GK GN+IMVTTR+ +VATMVEPISI+ L KLS+DQCWALFKES
Sbjct: 301 GKPGNTIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKES 342

BLAST of MELO.jh101542.1 vs. NCBI nr
Match: XP_038877687.1 (putative disease resistance protein RGA1 [Benincasa hispida] >XP_038877688.1 putative disease resistance protein RGA1 [Benincasa hispida])

HSP 1 Score: 488 bits (1255), Expect = 7.12e-163
Identity = 252/343 (73.47%), Postives = 281/343 (81.92%), Query Frame = 0

Query: 1   MAEFLWTFAVQEVLKKIVKYGAEQIVVAWELEDEVSLLKDKLHDADAILEDINRKKSHPG 60
           MAEFLWTFAVQEVLKKIVK+GAEQI +AW LE EVS L++ L  A+ IL DIN+KK H  
Sbjct: 1   MAEFLWTFAVQEVLKKIVKFGAEQIGLAWGLEKEVSQLRNWLLKAETILADINKKKLHIA 60

Query: 61  NSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTDKFRKVRDSISPSKNSFLFRRKMA 120
            SV+ WVE L+DIV+EADDLLDELVYEHLRR VE T K R+V DSIS SKN FLFRRK A
Sbjct: 61  -SVRLWVEDLQDIVYEADDLLDELVYEHLRRAVEQTGKLREVCDSISLSKNPFLFRRKKA 120

Query: 121 KKIKNITDTLYKHYCAASAFGLVGVESVTETELALRQIRETTSILDFQVEGREAEALELI 180
           K++KNIT  LYKHYC AS  GLV  ES TETE+ L QIRETTSILDF+V GREAE  E++
Sbjct: 121 KQMKNITQALYKHYCEASPLGLVSDESATETEVTLAQIRETTSILDFKVRGREAEFQEIL 180

Query: 181 KLAIDSANEHHLSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFTVTKIL 240
           KL I+S+NEH +SVISIVGMGGLGKTTLAKM+FNHDAIK HFD+TIWVCVSKPF V KIL
Sbjct: 181 KLVIESSNEHEMSVISIVGMGGLGKTTLAKMVFNHDAIKGHFDKTIWVCVSKPFIVMKIL 240

Query: 241 EQIFQGLTNNCSGLESNKEALLGRLRKEMQDKKYFLVLDDVWDDEKRLWDELCGCLKHIA 300
           E IFQG TN CSGL S KEALL RLR+ MQ K+YFLVLDDVWD +  LWDEL GCLK I 
Sbjct: 241 EAIFQGSTNTCSGLNS-KEALLKRLREAMQGKRYFLVLDDVWDKDNGLWDELSGCLKQIN 300

Query: 301 GKHGNSIMVTTRDVKVATMVEPISIHHLTKLSDDQCWALFKES 343
           GK GN +MVTTR V+VATMV  +S+HHL +LSDDQCW LFKES
Sbjct: 301 GKSGNCVMVTTRSVEVATMVNTVSVHHLKELSDDQCWVLFKES 341

BLAST of MELO.jh101542.1 vs. NCBI nr
Match: XP_004140442.1 (disease resistance protein RGA2 [Cucumis sativus] >KGN50811.1 hypothetical protein Csa_018865 [Cucumis sativus])

HSP 1 Score: 489 bits (1259), Expect = 1.35e-161
Identity = 255/343 (74.34%), Postives = 284/343 (82.80%), Query Frame = 0

Query: 1   MAEFLWTFAVQEVLKKIVKYGAEQIVVAWELEDEVSLLKDKLHDADAILEDINRKKSHPG 60
           MAEFLWTFAVQEVLKKIV +GAEQI +AW LE E+S LK  L  A  IL DIN KKSH  
Sbjct: 1   MAEFLWTFAVQEVLKKIVNFGAEQISLAWGLEKELSHLKKWLLKAQTILADINTKKSHH- 60

Query: 61  NSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTDKFRKVRDSISPSKNSFLFRRKMA 120
           +SV  WVE+L DI++EADDLLDE+VYE +R+TVE T K RKVRDSISPSKNSFLF  KMA
Sbjct: 61  HSVGLWVEELHDIIYEADDLLDEIVYEQIRQTVEQTGKLRKVRDSISPSKNSFLFGLKMA 120

Query: 121 KKIKNITDTLYKHYCAASAFGLVGVESVTETELALRQIRETTSILDFQVEGREAEALELI 180
           KK+K IT TLY+HYC AS  GLVG ES TE+E AL QIRETTSILDF+VEGREAE LE++
Sbjct: 121 KKMKKITKTLYEHYCEASPLGLVGDESTTESEAALNQIRETTSILDFEVEGREAEVLEIL 180

Query: 181 KLAIDSANEHHLSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFTVTKIL 240
           KL IDS +E H+SVISIVGMGGLGKTTLAKM+FNHDAIK HFD+T+WVCVSKPF V KIL
Sbjct: 181 KLVIDSTDEDHISVISIVGMGGLGKTTLAKMVFNHDAIKGHFDKTVWVCVSKPFIVMKIL 240

Query: 241 EQIFQGLTNNCSGLESNKEALLGRLRKEMQDKKYFLVLDDVWDDEKRLWDELCGCLKHIA 300
           E IFQGLTN  SGL S +EALL RLR+EMQ KKYFLVLDDVWD E  LWDEL G LK+IA
Sbjct: 241 EAIFQGLTNTSSGLNS-REALLNRLREEMQGKKYFLVLDDVWDKENCLWDELIGNLKYIA 300

Query: 301 GKHGNSIMVTTRDVKVATMVEPISIHHLTKLSDDQCWALFKES 343
           GK GNSIMVTTR V+VATMV+ + I+HL KLSDD CWAL K+S
Sbjct: 301 GKSGNSIMVTTRSVEVATMVKTVPIYHLKKLSDDHCWALLKKS 341

BLAST of MELO.jh101542.1 vs. NCBI nr
Match: XP_038877689.1 (putative disease resistance protein RGA3 [Benincasa hispida])

HSP 1 Score: 483 bits (1242), Expect = 1.54e-158
Identity = 253/342 (73.98%), Postives = 283/342 (82.75%), Query Frame = 0

Query: 1   MAEFLWTFAVQEVLKKIVKYGAEQIVVAWELEDEVSLLKDKLHDADAILEDINRKKSHPG 60
           MAEFLWTFAVQE+LKKIVK+GAEQI VAW LE E   LK  L  A+ IL DIN+KK H  
Sbjct: 1   MAEFLWTFAVQEILKKIVKFGAEQIGVAWGLEKEEFQLKKWLLKAETILADINKKKLHH- 60

Query: 61  NSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTDKFRKVRDSISPSKNSFLFRRKMA 120
           ++V+ WVE L+DIV+EADDLLDEL YE LRRT E T KF+KVRDSISPSKNSFLFR KMA
Sbjct: 61  HAVRLWVEDLQDIVYEADDLLDELRYEDLRRTGEQTGKFKKVRDSISPSKNSFLFRLKMA 120

Query: 121 KKIKNITDTLYKHYCAASAFGLVGVESVTETELALRQIRETTSILDFQVEGREAEALELI 180
            K+K IT  L+KHYC AS  GLVG ES TE E+AL+QIRETTSILDF+V GRE E LE++
Sbjct: 121 NKMKKITAALHKHYCEASPLGLVGEESATEIEVALQQIRETTSILDFEVVGREDEVLEIL 180

Query: 181 KLAIDSANEHHLSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFTVTKIL 240
           KL IDS+NEH +S+ISIVGMGGLGKTTLAKM+FNHDAIK HFDQTIWVCVSKPF V KIL
Sbjct: 181 KLVIDSSNEHDMSMISIVGMGGLGKTTLAKMVFNHDAIKGHFDQTIWVCVSKPFIVMKIL 240

Query: 241 EQIFQGLTNNCSGLESNKEALLGRLRKEMQDKKYFLVLDDVWDDEKRLWDELCGCLKHIA 300
           E IFQ LTN+ SGL S KEALL RLRKEMQ KKYFLVLDDVW+ E  LWDEL G LK IA
Sbjct: 241 EAIFQSLTNSSSGLNS-KEALLQRLRKEMQSKKYFLVLDDVWEKENDLWDELNGYLKQIA 300

Query: 301 GKHGNSIMVTTRDVKVATMVEPISIHHLTKLSDDQCWALFKE 342
           GK GNSIMVTTR+V+VATMV+ +SIHHL  LSDD CW+LF++
Sbjct: 301 GKSGNSIMVTTRNVEVATMVKTVSIHHLQTLSDDNCWSLFEK 340

BLAST of MELO.jh101542.1 vs. NCBI nr
Match: XP_022145177.1 (putative disease resistance protein RGA3 [Momordica charantia])

HSP 1 Score: 422 bits (1085), Expect = 4.42e-136
Identity = 220/343 (64.14%), Postives = 263/343 (76.68%), Query Frame = 0

Query: 1   MAEFLWTFAVQEVLKKIVKYGAEQIVVAWELEDEVSLLKDKLHDADAILEDINRKKSHPG 60
           MAEFLWTFAVQEVL+KIVK  A+QI +AW LE E+S L   L  A+ IL DINR+K H  
Sbjct: 1   MAEFLWTFAVQEVLRKIVKVAAQQIGLAWGLEKELSKLTKWLLKAETILGDINRRKLHH- 60

Query: 61  NSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTDKFRKVRDSISPSKNSFLFRRKMA 120
           +SV+ W+E L+D+VHEA DLLDELVYE LRR VE   K  KVR SIS S N F FRRKMA
Sbjct: 61  DSVRLWMEDLQDVVHEAHDLLDELVYEDLRRKVE-IGKMNKVRGSISRSYNLFSFRRKMA 120

Query: 121 KKIKNITDTLYKHYCAASAFGLVGVESVTETELALRQIRETTSILDFQVEGREAEALELI 180
           KK+K++T+TLYKHYC AS  GLVG ES+   ++ L+QIRETTS L+F+V GRE E   ++
Sbjct: 121 KKMKDVTETLYKHYCEASPLGLVGDESIETNQIVLKQIRETTSNLNFEVVGREIEVSNIV 180

Query: 181 KLAIDSANEHHLSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFTVTKIL 240
           KL IDS+NEHH S+I IVGMGGLGKTTLA+MIFNH+ IK HFD+TIW+CVS+PF +  IL
Sbjct: 181 KLVIDSSNEHHTSIIPIVGMGGLGKTTLAQMIFNHEVIKGHFDKTIWICVSEPFIIINIL 240

Query: 241 EQIFQGLTNNCSGLESNKEALLGRLRKEMQDKKYFLVLDDVWDDEKRLWDELCGCLKHIA 300
           E IFQGLT   SGL S KE L+ +L++EM  KKYFLVLDDVW++E  LWD+L  CLK I 
Sbjct: 241 EGIFQGLTKTSSGLNS-KETLVQKLQEEMHGKKYFLVLDDVWNEESGLWDDLGDCLKQIT 300

Query: 301 GKHGNSIMVTTRDVKVATMVEPISIHHLTKLSDDQCWALFKES 343
            K GN I+VTTR ++VAT +E +S HHL KLSDDQ W LFK S
Sbjct: 301 EKSGNCIIVTTRSLEVATTIETVSSHHLKKLSDDQSWFLFKGS 340

BLAST of MELO.jh101542.1 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 4.9e-39
Identity = 105/335 (31.34%), Postives = 179/335 (53.43%), Query Frame = 0

Query: 10  VQEVLKKIVKYGAEQIVVAWELEDEVSLLKDKLHDADAILEDINRKKSHPGNSVKRWVEK 69
           +Q +L  +  +   ++V+ +  +DE   L        A+LED   K+ +    ++ W++K
Sbjct: 6   IQVLLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLN-NKPLENWLQK 65

Query: 70  LEDIVHEADDLLDELVYEHLRRTVEHTDKFRKVRDSISPSKNSFLFRRKMAKKIKNITDT 129
           L    +E DD+LDE      + T     ++ +    + P      FR K+ K++  +   
Sbjct: 66  LNAATYEVDDILDEY---KTKATRFSQSEYGRYHPKVIP------FRHKVGKRMDQVMKK 125

Query: 130 LYKHYCAASAFGLVGVESVTETELALRQIRETTSIL-DFQVEGREAEALELIKLAIDSAN 189
           L         F L   E + E + A+R  RET S+L + QV GR+ E  E++K+ I++ +
Sbjct: 126 LKAIAEERKNFHL--HEKIVERQ-AVR--RETGSVLTEPQVYGRDKEKDEIVKILINNVS 185

Query: 190 E-HHLSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFTVTKILEQIFQGL 249
           +  HLSV+ I+GMGGLGKTTLA+M+FN   +  HF   IW+CVS+ F   ++++ I + +
Sbjct: 186 DAQHLSVLPILGMGGLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFDEKRLIKAIVESI 245

Query: 250 TNNCSGLESNKEALLGRLRKEMQDKKYFLVLDDVWDDEKRLWDELCGCLKHIAGKHGNSI 309
                  E +   L  +L++ +  K+Y LVLDDVW+++++ W  L   LK   G  G S+
Sbjct: 246 EGRPLLGEMDLAPLQKKLQELLNGKRYLLVLDDVWNEDQQKWANLRAVLK--VGASGASV 305

Query: 310 MVTTRDVKVATMVEPISIHHLTKLSDDQCWALFKE 343
           + TTR  KV +++  +  + L+ LS + CW LF +
Sbjct: 306 LTTTRLEKVGSIMGTLQPYELSNLSQEDCWLLFMQ 323

BLAST of MELO.jh101542.1 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 156.0 bits (393), Expect = 7.9e-37
Identity = 104/334 (31.14%), Postives = 176/334 (52.69%), Query Frame = 0

Query: 10  VQEVLKKIVKYGAEQIVVAWELEDEVSLLKDKLHDADAILEDINRKKSHPGNSVKRWVEK 69
           +Q +L  +  +   ++ + +  E E   L        A+LED  ++K     ++K W++K
Sbjct: 6   LQVLLDNLTFFIQGELGLVFGFEKEFKKLSSMFSMIQAVLEDA-QEKQLKYKAIKNWLQK 65

Query: 70  LEDIVHEADDLLDELVYEHLRRTVEHTDKFRKVRDSISPSKNSFLFRRKMAKKIKNITDT 129
           L    +E DD+LD+   E  R         + V     P   +F +  K+ K++K + + 
Sbjct: 66  LNVAAYEVDDILDDCKTEAARFK-------QAVLGRYHPRTITFCY--KVGKRMKEMMEK 125

Query: 130 LYKHYCAASAFGLVGVESVTETELALRQIRETTSILDFQVEGREAEALELIKLAIDSAN- 189
           L         F L   E + E + A RQ      + + +V GRE E  E++K+ I++ + 
Sbjct: 126 LDAIAEERRNFHL--DERIIERQAARRQTGFV--LTEPKVYGREKEEDEIVKILINNVSY 185

Query: 190 EHHLSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFTVTKILEQIFQGLT 249
              + V+ I+GMGGLGKTTLA+M+FN   I  HF+  IWVCVS  F   ++++ I + + 
Sbjct: 186 SEEVPVLPILGMGGLGKTTLAQMVFNDQRITEHFNLKIWVCVSDDFDEKRLIKAIVESIE 245

Query: 250 NNCSGLESNKEALLGRLRKEMQDKKYFLVLDDVWDDEKRLWDELCGCLKHIAGKHGNSIM 309
               G + +   L  +L++ +  K+YFLVLDDVW++++  WD L   LK   G  G SI+
Sbjct: 246 GKSLG-DMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQEKWDNLRAVLK--IGASGASIL 305

Query: 310 VTTRDVKVATMVEPISIHHLTKLSDDQCWALFKE 343
           +TTR  K+ +++  + ++ L+ LS + CW LFK+
Sbjct: 306 ITTRLEKIGSIMGTLQLYQLSNLSQEDCWLLFKQ 322

BLAST of MELO.jh101542.1 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 154.1 bits (388), Expect = 3.0e-36
Identity = 102/335 (30.45%), Postives = 175/335 (52.24%), Query Frame = 0

Query: 10  VQEVLKKIVKYGAEQIVVAWELEDEVSLLKDKLHDADAILEDINRKKSHPGNSVKRWVEK 69
           +Q VL  +  +   ++V+ +  +DE   L        A+LED   K+ +    ++ W++K
Sbjct: 6   IQVVLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLN-DKPLENWLQK 65

Query: 70  LEDIVHEADDLLDELVYEHLRRTVEHTDKFRKVRDSISPSKNSFLFRRKMAKKIKNITDT 129
           L    +E DD+LDE      + T     ++ +    + P      FR K+ K++  +   
Sbjct: 66  LNAATYEVDDILDEY---KTKATRFLQSEYGRYHPKVIP------FRHKVGKRMDQVMKK 125

Query: 130 LYKHYCAASAFGLVGVESVTETELALRQIRETTSIL-DFQVEGREAEALELIKLAIDSAN 189
           L         F L   E + E + A    RET S+L + QV GR+ E  E++K+ I++A+
Sbjct: 126 LNAIAEERKKFHL--QEKIIERQAA---TRETGSVLTEPQVYGRDKEKDEIVKILINTAS 185

Query: 190 E-HHLSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFTVTKILEQIFQGL 249
           +   LSV+ I+GMGGLGKTTL++M+FN   +   F   IW+C+S  F   ++++ I + +
Sbjct: 186 DAQKLSVLPILGMGGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKAIVESI 245

Query: 250 TNNCSGLESNKEALLGRLRKEMQDKKYFLVLDDVWDDEKRLWDELCGCLKHIAGKHGNSI 309
               S  + +   L  +L++ +  K+YFLVLDDVW++++  W  L   LK   G  G  +
Sbjct: 246 EGK-SLSDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQHKWANLRAVLK--VGASGAFV 305

Query: 310 MVTTRDVKVATMVEPISIHHLTKLSDDQCWALFKE 343
           + TTR  KV +++  +  + L+ LS + CW LF +
Sbjct: 306 LTTTRLEKVGSIMGTLQPYELSNLSPEDCWFLFMQ 322

BLAST of MELO.jh101542.1 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 2.1e-34
Identity = 103/335 (30.75%), Postives = 174/335 (51.94%), Query Frame = 0

Query: 10  VQEVLKKIVKYGAEQIVVAWELEDEVSLLKDKLHDADAILEDINRKKSHPGNSVKRWVEK 69
           +Q +L+ +  +  +++V+ +  E E   L        A+L+D  ++K     +++ W++K
Sbjct: 6   LQVLLENLTSFIGDKLVLIFGFEKECEKLSSVFSTIQAVLQDA-QEKQLKDKAIENWLQK 65

Query: 70  LEDIVHEADDLLDELVYEHLRRTVEHTDKFRKVRDSISPSKNSFLFRRKMAKKIKNITDT 129
           L    +E DD+L E   E +R        F + R           FR K+ +++K I + 
Sbjct: 66  LNSAAYEVDDILGECKNEAIR--------FEQSRLGFY-HPGIINFRHKIGRRMKEIMEK 125

Query: 130 LYKHYCAASAFGLVGVESVTETELALRQIRETTSIL-DFQVEGREAEALELIKLAIDSAN 189
           L         F    +E +TE + A    RET  +L + +V GR+ E  E++K+ I++ N
Sbjct: 126 LDAISEERRKFHF--LEKITERQ-AAAATRETGFVLTEPKVYGRDKEEDEIVKILINNVN 185

Query: 190 -EHHLSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFTVTKILEQIFQGL 249
               L V  I+GMGGLGKTTLA+MIFN + + +HF+  IWVCVS  F   ++++ I   +
Sbjct: 186 VAEELPVFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRLIKTIIGNI 245

Query: 250 TNNCSGLESNKEALLGRLRKEMQDKKYFLVLDDVWDDEKRLWDELCGCLKHIAGKHGNSI 309
             +   +E +  +   +L++ +  K+Y LVLDDVW+D+   W +L   L    G  G SI
Sbjct: 246 ERSSPHVE-DLASFQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVL--TVGARGASI 305

Query: 310 MVTTRDVKVATMVEPISIHHLTKLSDDQCWALFKE 343
           + TTR  KV +++  +  +HL+ LS      LF +
Sbjct: 306 LATTRLEKVGSIMGTLQPYHLSNLSPHDSLLLFMQ 324

BLAST of MELO.jh101542.1 vs. ExPASy Swiss-Prot
Match: P0C8S1 (Probable disease resistance RPP8-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RPP8L2 PE=3 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.7e-31
Identity = 98/343 (28.57%), Postives = 178/343 (51.90%), Query Frame = 0

Query: 17  IVKYGAEQIVVAWEL-----------EDEVSLLKDKLHDADAILEDINRKKSHPGNSVKR 76
           +V +G E++   WEL           +++V  LK +L    ++L+D + KK+     V+ 
Sbjct: 5   VVSFGVEKL---WELLSRESARLNGIDEQVDGLKRQLGRLQSLLKDADAKKNET-ERVRN 64

Query: 77  WVEKLEDIVHEADDLLDELVYEHLRRTVEHTDKFRKVRDSISPSKNSFLFRRKMAKKIKN 136
           ++E ++DIV++ADD+++  +   LR       K + ++  +       + RRK A  I+ 
Sbjct: 65  FLEDVKDIVYDADDIIESFLLNELR------GKEKGIKKQVRTLACFLVDRRKFASDIEG 124

Query: 137 ITDTLYKHYCAASAFGLVGVESVTETELAL----RQIRETTS-ILDFQVEGREAEALELI 196
           IT  + +      + G+  +       L+L    R+IR+T S   +  + G +    EL+
Sbjct: 125 ITKRISEVIVGMQSLGIQHIADGGGRSLSLQERQREIRQTFSRNSESDLVGLDQSVEELV 184

Query: 197 KLAIDSANEHHLSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFTVTKIL 256
              +++ +   + V+S+ GMGG+GKTTLA+ +F+HD ++RHFD   WVCVS+ FT   + 
Sbjct: 185 DHLVENDS---VQVVSVSGMGGIGKTTLARQVFHHDIVRRHFDGFSWVCVSQQFTRKDVW 244

Query: 257 EQIFQGLTNNCSG-LESNKEALLGRLRKEMQDKKYFLVLDDVWDDEKRLWDELCGCLKHI 316
           ++I Q L     G ++ ++  L G L + ++  +Y LVLDDVW +E   WD +     H 
Sbjct: 245 QRILQDLRPYDEGIIQMDEYTLQGELFELLESGRYLLVLDDVWKEED--WDRIKAVFPH- 304

Query: 317 AGKHGNSIMVTTRDVKVATMVEPISIHHLTK-LSDDQCWALFK 342
             K G  +++T+R+  +    +P       + L+ +Q W LF+
Sbjct: 305 --KRGWKMLLTSRNEGLGLHADPTCFAFRPRILTPEQSWKLFE 329

BLAST of MELO.jh101542.1 vs. ExPASy TrEMBL
Match: A0A0A0LBV0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G822360 PE=4 SV=1)

HSP 1 Score: 588 bits (1517), Expect = 1.79e-200
Identity = 300/343 (87.46%), Postives = 317/343 (92.42%), Query Frame = 0

Query: 1   MAEFLWTFAVQEVLKKIVKYGAEQIVVAWELEDEVSLLKDKLHDADAILEDINRKKSHPG 60
           MAE + TF VQEVLK+IVKYGAEQIVVAWELE+EVSLLKDKLHDAD ILEDINRKKSHPG
Sbjct: 1   MAE-IGTFVVQEVLKRIVKYGAEQIVVAWELENEVSLLKDKLHDADTILEDINRKKSHPG 60

Query: 61  NSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTDKFRKVRDSISPSKNSFLFRRKMA 120
           NSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHT+KF KV DSIS S NSFLFRRKMA
Sbjct: 61  NSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTEKFSKVSDSISSSINSFLFRRKMA 120

Query: 121 KKIKNITDTLYKHYCAASAFGLVGVESVTETELALRQIRETTSILDFQVEGREAEALELI 180
           KKIKNITDTL +HYCAASAFGLVGVE+VTE ELAL QIRETTSILDFQVEGREAE LEL+
Sbjct: 121 KKIKNITDTLNQHYCAASAFGLVGVETVTEIELALNQIRETTSILDFQVEGREAEVLELL 180

Query: 181 KLAIDSANEHHLSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFTVTKIL 240
           KLAIDS NEHH+SVISIVGMGGLGKTTLAKMIFNH  I+ HFD+TIWVCVSKPF VTKIL
Sbjct: 181 KLAIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVTKIL 240

Query: 241 EQIFQGLTNNCSGLESNKEALLGRLRKEMQDKKYFLVLDDVWDDEKRLWDELCGCLKHIA 300
           E+IFQGLT  CSGLESNKEALLGRLRKEMQDK YFLVLDDVWD+EK LWDEL GCLKHIA
Sbjct: 241 EKIFQGLTKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRGCLKHIA 300

Query: 301 GKHGNSIMVTTRDVKVATMVEPISIHHLTKLSDDQCWALFKES 343
           GK GN+IMVTTR+ +VATMVEPISI+ L KLS+DQCWALFKES
Sbjct: 301 GKPGNTIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKES 342

BLAST of MELO.jh101542.1 vs. ExPASy TrEMBL
Match: A0A0A0KST5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G266890 PE=4 SV=1)

HSP 1 Score: 489 bits (1259), Expect = 6.54e-162
Identity = 255/343 (74.34%), Postives = 284/343 (82.80%), Query Frame = 0

Query: 1   MAEFLWTFAVQEVLKKIVKYGAEQIVVAWELEDEVSLLKDKLHDADAILEDINRKKSHPG 60
           MAEFLWTFAVQEVLKKIV +GAEQI +AW LE E+S LK  L  A  IL DIN KKSH  
Sbjct: 1   MAEFLWTFAVQEVLKKIVNFGAEQISLAWGLEKELSHLKKWLLKAQTILADINTKKSHH- 60

Query: 61  NSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTDKFRKVRDSISPSKNSFLFRRKMA 120
           +SV  WVE+L DI++EADDLLDE+VYE +R+TVE T K RKVRDSISPSKNSFLF  KMA
Sbjct: 61  HSVGLWVEELHDIIYEADDLLDEIVYEQIRQTVEQTGKLRKVRDSISPSKNSFLFGLKMA 120

Query: 121 KKIKNITDTLYKHYCAASAFGLVGVESVTETELALRQIRETTSILDFQVEGREAEALELI 180
           KK+K IT TLY+HYC AS  GLVG ES TE+E AL QIRETTSILDF+VEGREAE LE++
Sbjct: 121 KKMKKITKTLYEHYCEASPLGLVGDESTTESEAALNQIRETTSILDFEVEGREAEVLEIL 180

Query: 181 KLAIDSANEHHLSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFTVTKIL 240
           KL IDS +E H+SVISIVGMGGLGKTTLAKM+FNHDAIK HFD+T+WVCVSKPF V KIL
Sbjct: 181 KLVIDSTDEDHISVISIVGMGGLGKTTLAKMVFNHDAIKGHFDKTVWVCVSKPFIVMKIL 240

Query: 241 EQIFQGLTNNCSGLESNKEALLGRLRKEMQDKKYFLVLDDVWDDEKRLWDELCGCLKHIA 300
           E IFQGLTN  SGL S +EALL RLR+EMQ KKYFLVLDDVWD E  LWDEL G LK+IA
Sbjct: 241 EAIFQGLTNTSSGLNS-REALLNRLREEMQGKKYFLVLDDVWDKENCLWDELIGNLKYIA 300

Query: 301 GKHGNSIMVTTRDVKVATMVEPISIHHLTKLSDDQCWALFKES 343
           GK GNSIMVTTR V+VATMV+ + I+HL KLSDD CWAL K+S
Sbjct: 301 GKSGNSIMVTTRSVEVATMVKTVPIYHLKKLSDDHCWALLKKS 341

BLAST of MELO.jh101542.1 vs. ExPASy TrEMBL
Match: A0A6J1CVR6 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111014685 PE=4 SV=1)

HSP 1 Score: 422 bits (1085), Expect = 2.14e-136
Identity = 220/343 (64.14%), Postives = 263/343 (76.68%), Query Frame = 0

Query: 1   MAEFLWTFAVQEVLKKIVKYGAEQIVVAWELEDEVSLLKDKLHDADAILEDINRKKSHPG 60
           MAEFLWTFAVQEVL+KIVK  A+QI +AW LE E+S L   L  A+ IL DINR+K H  
Sbjct: 1   MAEFLWTFAVQEVLRKIVKVAAQQIGLAWGLEKELSKLTKWLLKAETILGDINRRKLHH- 60

Query: 61  NSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTDKFRKVRDSISPSKNSFLFRRKMA 120
           +SV+ W+E L+D+VHEA DLLDELVYE LRR VE   K  KVR SIS S N F FRRKMA
Sbjct: 61  DSVRLWMEDLQDVVHEAHDLLDELVYEDLRRKVE-IGKMNKVRGSISRSYNLFSFRRKMA 120

Query: 121 KKIKNITDTLYKHYCAASAFGLVGVESVTETELALRQIRETTSILDFQVEGREAEALELI 180
           KK+K++T+TLYKHYC AS  GLVG ES+   ++ L+QIRETTS L+F+V GRE E   ++
Sbjct: 121 KKMKDVTETLYKHYCEASPLGLVGDESIETNQIVLKQIRETTSNLNFEVVGREIEVSNIV 180

Query: 181 KLAIDSANEHHLSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFTVTKIL 240
           KL IDS+NEHH S+I IVGMGGLGKTTLA+MIFNH+ IK HFD+TIW+CVS+PF +  IL
Sbjct: 181 KLVIDSSNEHHTSIIPIVGMGGLGKTTLAQMIFNHEVIKGHFDKTIWICVSEPFIIINIL 240

Query: 241 EQIFQGLTNNCSGLESNKEALLGRLRKEMQDKKYFLVLDDVWDDEKRLWDELCGCLKHIA 300
           E IFQGLT   SGL S KE L+ +L++EM  KKYFLVLDDVW++E  LWD+L  CLK I 
Sbjct: 241 EGIFQGLTKTSSGLNS-KETLVQKLQEEMHGKKYFLVLDDVWNEESGLWDDLGDCLKQIT 300

Query: 301 GKHGNSIMVTTRDVKVATMVEPISIHHLTKLSDDQCWALFKES 343
            K GN I+VTTR ++VAT +E +S HHL KLSDDQ W LFK S
Sbjct: 301 EKSGNCIIVTTRSLEVATTIETVSSHHLKKLSDDQSWFLFKGS 340

BLAST of MELO.jh101542.1 vs. ExPASy TrEMBL
Match: A0A6J1CVR3 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111014682 PE=4 SV=1)

HSP 1 Score: 408 bits (1048), Expect = 7.39e-136
Identity = 215/344 (62.50%), Postives = 263/344 (76.45%), Query Frame = 0

Query: 1   MAEFLWTFAVQEVLKKIVKYGAEQIVVAWELEDEVSLLKDKLHDADAILEDINRKKSHPG 60
           MAEFLWTFAVQEVLKK++K  AEQI VAW L+ E+S L+ +L  A+ IL DINRKK    
Sbjct: 1   MAEFLWTFAVQEVLKKVLKLAAEQIGVAWGLDKELSKLRKRLLKAETILGDINRKKLRH- 60

Query: 61  NSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTDKFRKVRDSISPSKNSFLFRRKMA 120
            SV+ WVE L+ +V++ADDLLDELVYE LRR VE  +K  KVR+  S + N FLFR KMA
Sbjct: 61  ESVRLWVEDLQHLVYQADDLLDELVYEDLRRKVE-IEKINKVRNFFSLTNNPFLFRSKMA 120

Query: 121 KKIKNITDTLYKHYCAASAFGLVGVESV-TETELALRQIRETTSILDFQVEGREAEALEL 180
             +K I++TLY+HY  ASA GLVG E+  T+ +  L+QIRETTSIL+F+V GRE E L +
Sbjct: 121 NSMKAISETLYQHYYEASALGLVGEETTETDNKNILKQIRETTSILNFEVIGREIEVLNI 180

Query: 181 IKLAIDSANEHHLSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFTVTKI 240
           +KL IDS+ EH +S+I IVGMGGLGKTTLAKM+FNH+ IK HFD++IWVCVS+ F +  I
Sbjct: 181 VKLVIDSSYEHRMSIIPIVGMGGLGKTTLAKMVFNHELIKEHFDKSIWVCVSESFIIINI 240

Query: 241 LEQIFQGLTNNCSGLESNKEALLGRLRKEMQDKKYFLVLDDVWDDEKRLWDELCGCLKHI 300
           LE IFQ LTN  SGL + KE LL RL+KEMQ KKYFLVLDDVW++E  LW EL  CL  I
Sbjct: 241 LEGIFQSLTNTSSGL-NYKETLLQRLQKEMQGKKYFLVLDDVWNEEVSLWGELRDCLNEI 300

Query: 301 AGKHGNSIMVTTRDVKVATMVEPISIHHLTKLSDDQCWALFKES 343
           AGK GNSI+VTTR ++VAT++  +  HH+ KLSDD+CW LFKES
Sbjct: 301 AGKSGNSIIVTTRSIEVATIMMTVPSHHIRKLSDDECWTLFKES 341

BLAST of MELO.jh101542.1 vs. ExPASy TrEMBL
Match: A0A6J1CU86 (disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111014619 PE=4 SV=1)

HSP 1 Score: 417 bits (1071), Expect = 7.68e-134
Identity = 219/343 (63.85%), Postives = 257/343 (74.93%), Query Frame = 0

Query: 1   MAEFLWTFAVQEVLKKIVKYGAEQIVVAWELEDEVSLLKDKLHDADAILEDINRKKSHPG 60
           MAEFLWTFAVQEVL+KIVK  A+QI +A  LE E+S L   L  A+ IL DINRKKSH  
Sbjct: 1   MAEFLWTFAVQEVLRKIVKVAAQQIGLARGLEKELSNLTKWLQKAETILRDINRKKSH-S 60

Query: 61  NSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTDKFRKVRDSISPSKNSFLFRRKMA 120
           +SV+ WVE L+D+VHEADDLLDELVYEHLRR VE   K  KVRDS S S N FLFR KMA
Sbjct: 61  DSVRLWVEDLQDVVHEADDLLDELVYEHLRRKVE-IGKINKVRDSFSTSHNVFLFRLKMA 120

Query: 121 KKIKNITDTLYKHYCAASAFGLVGVESVTETELALRQIRETTSILDFQVEGREAEALELI 180
           KKIK+IT  L+KHYC AS  GLVG ES    ++ L QIR+TTS L+F+V GRE E   ++
Sbjct: 121 KKIKDITQILHKHYCEASPLGLVGQESTEANQIVLSQIRDTTSNLNFEVVGREDEVSNIV 180

Query: 181 KLAIDSANEHHLSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFTVTKIL 240
           KL IDS+NEHH+S++ IVGMGGLGKTT A+ IFNHD IK HFD  IWVCVS+PF +  IL
Sbjct: 181 KLVIDSSNEHHMSILPIVGMGGLGKTTFAQKIFNHDTIKGHFDNIIWVCVSEPFIIINIL 240

Query: 241 EQIFQGLTNNCSGLESNKEALLGRLRKEMQDKKYFLVLDDVWDDEKRLWDELCGCLKHIA 300
           E IFQ LT   SGL ++K  LL +++KEM  K YFLVLDDVW++E  LWD+L  CLK IA
Sbjct: 241 EGIFQSLTKTSSGL-NHKGVLLEKIQKEMHGKMYFLVLDDVWNEESGLWDDLGDCLKQIA 300

Query: 301 GKHGNSIMVTTRDVKVATMVEPISIHHLTKLSDDQCWALFKES 343
           GK GN I+VTTR ++VA +V  +  H L KLSDDQCW LFKES
Sbjct: 301 GKSGNCIIVTTRSLEVANIVGTVPSHRLKKLSDDQCWFLFKES 340

BLAST of MELO.jh101542.1 vs. TAIR 10
Match: AT1G53350.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 138.3 bits (347), Expect = 1.2e-32
Identity = 98/343 (28.57%), Postives = 178/343 (51.90%), Query Frame = 0

Query: 17  IVKYGAEQIVVAWEL-----------EDEVSLLKDKLHDADAILEDINRKKSHPGNSVKR 76
           +V +G E++   WEL           +++V  LK +L    ++L+D + KK+     V+ 
Sbjct: 5   VVSFGVEKL---WELLSRESARLNGIDEQVDGLKRQLGRLQSLLKDADAKKNET-ERVRN 64

Query: 77  WVEKLEDIVHEADDLLDELVYEHLRRTVEHTDKFRKVRDSISPSKNSFLFRRKMAKKIKN 136
           ++E ++DIV++ADD+++  +   LR       K + ++  +       + RRK A  I+ 
Sbjct: 65  FLEDVKDIVYDADDIIESFLLNELR------GKEKGIKKQVRTLACFLVDRRKFASDIEG 124

Query: 137 ITDTLYKHYCAASAFGLVGVESVTETELAL----RQIRETTS-ILDFQVEGREAEALELI 196
           IT  + +      + G+  +       L+L    R+IR+T S   +  + G +    EL+
Sbjct: 125 ITKRISEVIVGMQSLGIQHIADGGGRSLSLQERQREIRQTFSRNSESDLVGLDQSVEELV 184

Query: 197 KLAIDSANEHHLSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFTVTKIL 256
              +++ +   + V+S+ GMGG+GKTTLA+ +F+HD ++RHFD   WVCVS+ FT   + 
Sbjct: 185 DHLVENDS---VQVVSVSGMGGIGKTTLARQVFHHDIVRRHFDGFSWVCVSQQFTRKDVW 244

Query: 257 EQIFQGLTNNCSG-LESNKEALLGRLRKEMQDKKYFLVLDDVWDDEKRLWDELCGCLKHI 316
           ++I Q L     G ++ ++  L G L + ++  +Y LVLDDVW +E   WD +     H 
Sbjct: 245 QRILQDLRPYDEGIIQMDEYTLQGELFELLESGRYLLVLDDVWKEED--WDRIKAVFPH- 304

Query: 317 AGKHGNSIMVTTRDVKVATMVEPISIHHLTK-LSDDQCWALFK 342
             K G  +++T+R+  +    +P       + L+ +Q W LF+
Sbjct: 305 --KRGWKMLLTSRNEGLGLHADPTCFAFRPRILTPEQSWKLFE 329

BLAST of MELO.jh101542.1 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 135.6 bits (340), Expect = 7.8e-32
Identity = 91/321 (28.35%), Postives = 183/321 (57.01%), Query Frame = 0

Query: 32  EDEVSLLKDKLHDADAILEDINRKK-SHPGNSVKRWVEKLEDIVHEADDLLDELVYEHLR 91
           E+ +  L   L    A+L D   K+ ++P   V++WV +L D+V+ A+D LD++  E LR
Sbjct: 36  ENLLERLSTALLTITAVLIDAEEKQITNP--VVEKWVNELRDVVYHAEDALDDIATEALR 95

Query: 92  RTV----EHTDKFRKVRDSISPSKNSFLFRRKMAKKIKNITDTLYKHYCAASAFGLVGVE 151
             +      +++ R++R  +S           +  +++ +T  L +    AS   ++G++
Sbjct: 96  LNIGAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLER---LASQRNILGLK 155

Query: 152 SVTETELALRQIRETTSILD-FQVEGREAEALELIKLAI-DSANEHHLSVISIVGMGGLG 211
            +  T +  +Q   TTS++D  +V GR+ +  E+++  I ++  ++ ++V++IVG+GG+G
Sbjct: 156 EL--TAMIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVG 215

Query: 212 KTTLAKMIFNHDAIKRHFDQTIWVCVSKPFTVTKILEQIFQGLTNNCSGLESNKEALLGR 271
           KTTL+++++N   ++ +F   +W  VS+ F V KI +++++ +T+      ++ + L  +
Sbjct: 216 KTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEF-TDLDVLQVK 275

Query: 272 LRKEMQDK--KYFLVLDDVWDDEKRLWDELCGCLKHIAGKHGNSIMVTTRDVKVATMVEP 331
           L++ +      + LVLDD+W++    WD L     H A   G+ I+VTTR  +VA+++  
Sbjct: 276 LKERLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAA--QGSQILVTTRSQRVASIMCA 335

Query: 332 ISIHHLTKLSDDQCWALFKES 344
           + +H+L  LSD  CW+LF ++
Sbjct: 336 VHVHNLQPLSDGDCWSLFMKT 346

BLAST of MELO.jh101542.1 vs. TAIR 10
Match: AT5G48620.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 129.0 bits (323), Expect = 7.3e-30
Identity = 92/318 (28.93%), Postives = 170/318 (53.46%), Query Frame = 0

Query: 31  LEDEVSLLKDKLHDADAILEDINRKKSHPGNSVKRWVEKLEDIVHEADDLLDELVYEHLR 90
           +++++  LK +L    ++L+D + KK H  + V+ ++E ++D+V +A+D+++  V   LR
Sbjct: 27  IDEQLDGLKRQLRSLQSLLKDADAKK-HGSDRVRNFLEDVKDLVFDAEDIIESYVLNKLR 86

Query: 91  RTVEHTDKFRKVRDSISPSKNSFLFRRKMAKKIKNITDTLYKHYCAASAFGLV----GVE 150
                  + + V+  +         R K+A  I+ IT  +        +FG+     GV 
Sbjct: 87  ------GEGKGVKKHVRRLARFLTDRHKVASDIEGITKRISDVIGEMQSFGIQQIIDGVR 146

Query: 151 SVT--ETELALRQIRET-TSILDFQVEGREAEALELIKLAIDSANEHHLSVISIVGMGGL 210
           S++  E +   R+IR+T     +  + G E    EL+   +++       V+SI GMGG+
Sbjct: 147 SLSLQERQRVQREIRQTYPDSSESDLVGVEQSVEELVGHLVEN---DIYQVVSIAGMGGI 206

Query: 211 GKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFTVTKILEQIFQGL-TNNCSGLESNKEALL 270
           GKTTLA+ +F+HD ++RHFD   WVCVS+ FT+  + ++I Q L  ++ + L+ ++ AL 
Sbjct: 207 GKTTLARQVFHHDLVRRHFDGFAWVCVSQQFTLKHVWQRILQELQPHDGNILQMDESALQ 266

Query: 271 GRLRKEMQDKKYFLVLDDVWDDEKRLWDELCGCLKHIAGKHGNSIMVTTRDVKVATMVEP 330
            +L + ++  +Y LVLDDVW  +K  WD +         K G  +++T+R+  V    +P
Sbjct: 267 PKLFQLLETGRYLLVLDDVW--KKEDWDRIKAVFPR---KRGWKMLLTSRNEGVGIHADP 326

Query: 331 ISI-HHLTKLSDDQCWAL 340
             +    + L+ ++ W L
Sbjct: 327 TCLTFRASILNPEESWKL 329

BLAST of MELO.jh101542.1 vs. TAIR 10
Match: AT5G35450.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 128.6 bits (322), Expect = 9.5e-30
Identity = 100/349 (28.65%), Postives = 188/349 (53.87%), Query Frame = 0

Query: 1   MAEFLWTFAVQEVLKKIVKYGAEQIVVAWELEDEVSLLKDKLHDADAILEDINRKKSHPG 60
           MAE + +F VQ+ L  ++   +E++     ++++V  LK +L    ++L+D + KK H  
Sbjct: 1   MAEGVVSFGVQK-LWALLNRESERLN---GIDEQVDGLKRQLRGLQSLLKDADAKK-HGS 60

Query: 61  NSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTDKFRKVRDSISPSKNSFLFRRKMA 120
           + V+ ++E ++D+V +A+D+++  V   LR       + + V++ +         R K+A
Sbjct: 61  DRVRNFLEDVKDLVFDAEDIIESYVLNKLR------GEGKGVKNHVRRLACFLTDRHKVA 120

Query: 121 KKIKNITDTLYKHYCAASAFGLV-----GVESVTETELALRQIRET-TSILDFQVEGREA 180
             I+ IT  + K      + G+      G  S++  ++  R+IR+T  +  +  + G E 
Sbjct: 121 SDIEGITKRISKVIGEMQSLGIQQQIIDGGRSLSLQDIQ-REIRQTFPNSSESDLVGVEQ 180

Query: 181 EALELIKLAIDSANEHHLSVISIVGMGGLGKTTLAKMIFNHDAIKRHFDQTIWVCVSKPF 240
              EL+   ++  N   + V+SI GMGG+GKTTLA+ IF+HD ++RHFD   WVCVS+ F
Sbjct: 181 SVEELVGPMVEIDN---IQVVSISGMGGIGKTTLARQIFHHDLVRRHFDGFAWVCVSQQF 240

Query: 241 TVTKILEQIFQGL-TNNCSGLESNKEALLGRLRKEMQDKKYFLVLDDVWDDEKRLWDELC 300
           T   + ++I Q L  ++   L+ ++  + G+L + ++  +Y +VLDDVW +E   WD + 
Sbjct: 241 TQKHVWQRILQELRPHDGEILQMDEYTIQGKLFQLLETGRYLVVLDDVWKEED--WDRI- 300

Query: 301 GCLKHIAGKHGNSIMVTTRDVKVATMVEPISIHHLTK-LSDDQCWALFK 342
              +    K G  +++T+R+  V    +P  +    + L+  + W LF+
Sbjct: 301 --KEVFPRKRGWKMLLTSRNEGVGLHADPTCLSFRARILNPKESWKLFE 329

BLAST of MELO.jh101542.1 vs. TAIR 10
Match: AT5G43470.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 124.8 bits (312), Expect = 1.4e-28
Identity = 91/318 (28.62%), Postives = 168/318 (52.83%), Query Frame = 0

Query: 31  LEDEVSLLKDKLHDADAILEDINRKKSHPGNSVKRWVEKLEDIVHEADDLLDELVYEHLR 90
           ++ ++  LK +L    ++L+D + KK H  + V+ ++E ++D+V +A+D+++  V   L 
Sbjct: 27  IDGQLDGLKRQLRSLQSLLKDADAKK-HGSDRVRNFLEDVKDLVFDAEDIIESYVLNKL- 86

Query: 91  RTVEHTDKFRKVRDSISPSKNSFLFRRKMAKKIKNITDTLYKHYCAASAFGLVGV----- 150
                + K + V+  +         R K+A  I+ IT  + +      +FG+  +     
Sbjct: 87  -----SGKGKGVKKHVRRLACFLTDRHKVASDIEGITKRISEVIGEMQSFGIQQIIDGGR 146

Query: 151 -ESVTETELALRQIRET-TSILDFQVEGREAEALELIKLAIDSANEHHLSVISIVGMGGL 210
             S+ E +   R+IR+T     +  + G E    EL+   ++  N+ H  V+SI GMGG+
Sbjct: 147 SLSLQERQRVQREIRQTYPDSSESDLVGVEQSVKELVGHLVE--NDVH-QVVSIAGMGGI 206

Query: 211 GKTTLAKMIFNHDAIKRHFDQTIWVCVSKPFTVTKILEQIFQGL-TNNCSGLESNKEALL 270
           GKTTLA+ +F+HD ++RHFD   WVCVS+ FT   + ++I Q L  ++   L+ ++ AL 
Sbjct: 207 GKTTLARQVFHHDLVRRHFDGFAWVCVSQQFTQKHVWQRILQELQPHDGDILQMDEYALQ 266

Query: 271 GRLRKEMQDKKYFLVLDDVWDDEKRLWDELCGCLKHIAGKHGNSIMVTTRDVKVATMVEP 330
            +L + ++  +Y +VLDDVW  +K  WD +         K G  +++T+R+  V    +P
Sbjct: 267 RKLFQLLEAGRYLVVLDDVW--KKEDWDVIKAVFPR---KRGWKMLLTSRNEGVGIHADP 326

Query: 331 ISI-HHLTKLSDDQCWAL 340
             +    + L+ ++ W L
Sbjct: 327 TCLTFRASILNPEESWKL 329

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004136783.23.69e-20087.46disease resistance protein RGA2 [Cucumis sativus][more]
XP_038877687.17.12e-16373.47putative disease resistance protein RGA1 [Benincasa hispida] >XP_038877688.1 put... [more]
XP_004140442.11.35e-16174.34disease resistance protein RGA2 [Cucumis sativus] >KGN50811.1 hypothetical prote... [more]
XP_038877689.11.54e-15873.98putative disease resistance protein RGA3 [Benincasa hispida][more]
XP_022145177.14.42e-13664.14putative disease resistance protein RGA3 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q7XBQ94.9e-3931.34Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 ... [more]
Q7XA407.9e-3731.14Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q7XA423.0e-3630.45Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q7XA392.1e-3430.75Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
P0C8S11.7e-3128.57Probable disease resistance RPP8-like protein 2 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A0A0LBV01.79e-20087.46Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G822360 PE=4 SV=1[more]
A0A0A0KST56.54e-16274.34Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G266890 PE=4 SV=1[more]
A0A6J1CVR62.14e-13664.14putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1CVR37.39e-13662.50putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1CU867.68e-13463.85disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111014... [more]
Match NameE-valueIdentityDescription
AT1G53350.11.2e-3228.57Disease resistance protein (CC-NBS-LRR class) family [more]
AT3G14470.17.8e-3228.35NB-ARC domain-containing disease resistance protein [more]
AT5G48620.17.3e-3028.93Disease resistance protein (CC-NBS-LRR class) family [more]
AT5G35450.19.5e-3028.65Disease resistance protein (CC-NBS-LRR class) family [more]
AT5G43470.11.4e-2828.62Disease resistance protein (CC-NBS-LRR class) family [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 63..87
NoneNo IPR availablePRINTSPR00364DISEASERSISTcoord: 268..282
score: 42.83
coord: 194..209
score: 56.41
NoneNo IPR availableGENE3D1.20.5.4130coord: 8..129
e-value: 2.9E-15
score: 58.2
NoneNo IPR availablePANTHERPTHR23155:SF860OS10G0130600 PROTEINcoord: 20..342
IPR041118Rx, N-terminalPFAMPF18052Rx_Ncoord: 9..98
e-value: 3.9E-15
score: 55.9
IPR002182NB-ARCPFAMPF00931NB-ARCcoord: 183..346
e-value: 1.3E-41
score: 142.5
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 154..324
e-value: 3.4E-39
score: 136.2
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 168..342
IPR044974Disease resistance protein, plantsPANTHERPTHR23155DISEASE RESISTANCE PROTEIN RPcoord: 20..342

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO.jh101542.1.t1MELO.jh101542.1.t1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
molecular_function GO:0043531 ADP binding