MELO.jh031516.1 (gene) Melon (Harukei-3) v1.41

Overview
NameMELO.jh031516.1
Typegene
OrganismCucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
DescriptionTrypsin inhibitor 1-like
Locationchr07: 27186177 .. 27186807 (+)
RNA-Seq ExpressionMELO.jh031516.1
SyntenyMELO.jh031516.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonstart_codonCDSpolypeptidestop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAGAGAAGTCCAAAGGGAAGAAAAGTGAGAAGAAATTAACAATGGAGATGAGTAAGAACACAGTTGGGTTTTTTAAGTGGAAAGTGATGATTATGATAGTACTGCTCTTGGGGACTTCAATTTCAGCCAACAACATGGTGGACGCAAATGCAATGGAAGTTGAAGAAGGGAATGGAATTTTTATGTTGCCAAGAAAAATGATGAAAAATGGATTACTCATATTTCAATATGGAGTTTGTCCTCTCATTTATATGCCATGTAAACAAGACTCCGACTGCTTGAAAGATTGTGTTTGCAAACAAACAGGTATATGCGGTGGAAAATATTGGTGACCTCAATCATATCATCTATTATATGCTTCCTTTCCTATAACAATTGCAGTTCCTTTTTTTTCTCTCTTTGTAAACTATGTGATGGCAGTTTTTACCCTTTCTTTCCATATGAATAAATATTAATCGAGTGTCATGTTTGTATGAGTCTAGTGAACGGTAAATTTGTTTGATTGTGGTTGGTCGACCTAATGCCTAAAAAACAAAATAATTGTGTGTCACAATGCAATGCCCTGCCACACATAGATGTTATGTATTCTAATGCTCGAGTTATTATCAAGTTTATGTGTGTACGTATAAA

mRNA sequence

AGAGAGAAGTCCAAAGGGAAGAAAAGTGAGAAGAAATTAACAATGGAGATGAGTAAGAACACAGTTGGGTTTTTTAAGTGGAAAGTGATGATTATGATAGTACTGCTCTTGGGGACTTCAATTTCAGCCAACAACATGGTGGACGCAAATGCAATGGAAGTTGAAGAAGGGAATGGAATTTTTATGTTGCCAAGAAAAATGATGAAAAATGGATTACTCATATTTCAATATGGAGTTTGTCCTCTCATTTATATGCCATGTAAACAAGACTCCGACTGCTTGAAAGATTGTGTTTGCAAACAAACAGGTATATGCGGTGGAAAATATTGGTGACCTCAATCATATCATCTATTATATGCTTCCTTTCCTATAACAATTGCAGTTCCTTTTTTTTCTCTCTTTGTAAACTATGTGATGGCAGTTTTTACCCTTTCTTTCCATATGAATAAATATTAATCGAGTGTCATGTTTGTATGAGTCTAGTGAACGGTAAATTTGTTTGATTGTGGTTGGTCGACCTAATGCCTAAAAAACAAAATAATTGTGTGTCACAATGCAATGCCCTGCCACACATAGATGTTATGTATTCTAATGCTCGAGTTATTATCAAGTTTATGTGTGTACGTATAAA

Coding sequence (CDS)

ATGGAGATGAGTAAGAACACAGTTGGGTTTTTTAAGTGGAAAGTGATGATTATGATAGTACTGCTCTTGGGGACTTCAATTTCAGCCAACAACATGGTGGACGCAAATGCAATGGAAGTTGAAGAAGGGAATGGAATTTTTATGTTGCCAAGAAAAATGATGAAAAATGGATTACTCATATTTCAATATGGAGTTTGTCCTCTCATTTATATGCCATGTAAACAAGACTCCGACTGCTTGAAAGATTGTGTTTGCAAACAAACAGGTATATGCGGTGGAAAATATTGGTGA

Protein sequence

MEMSKNTVGFFKWKVMIMIVLLLGTSISANNMVDANAMEVEEGNGIFMLPRKMMKNGLLIFQYGVCPLIYMPCKQDSDCLKDCVCKQTGICGGKYW
Homology
BLAST of MELO.jh031516.1 vs. NCBI nr
Match: KAG6576919.1 (hypothetical protein SDJN03_24493, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 62.4 bits (150), Expect = 9.43e-10
Identity = 34/75 (45.33%), Postives = 49/75 (65.33%), Query Frame = 0

Query: 20  VLLLGTSISANNMVDANAME-VEEGNGI-FMLPRKMMKNGLLIFQYGVCPLIYMPCKQDS 79
           +LL+ T  SA   +D ++M+ V +G  I   +PRK+MK G+L  +  VCP I M CK+DS
Sbjct: 67  ILLVATLSSAAYAIDDDSMDLVFDGRDINHGVPRKIMKWGVLNHEERVCPKILMECKKDS 126

Query: 80  DCLKDCVCKQTGICG 92
           DCL +C+C + G CG
Sbjct: 127 DCLAECICLEHGYCG 141

BLAST of MELO.jh031516.1 vs. NCBI nr
Match: KAG7014944.1 (hypothetical protein SDJN02_22575, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 62.4 bits (150), Expect = 9.43e-10
Identity = 34/75 (45.33%), Postives = 49/75 (65.33%), Query Frame = 0

Query: 20  VLLLGTSISANNMVDANAME-VEEGNGI-FMLPRKMMKNGLLIFQYGVCPLIYMPCKQDS 79
           +LL+ T  SA   +D ++M+ V +G  I   +PRK+MK G+L  +  VCP I M CK+DS
Sbjct: 67  ILLVATLSSAAYAIDDDSMDLVFDGRDINHGVPRKIMKWGVLNHEERVCPKILMECKKDS 126

Query: 80  DCLKDCVCKQTGICG 92
           DCL +C+C + G CG
Sbjct: 127 DCLAECICLEHGYCG 141

BLAST of MELO.jh031516.1 vs. NCBI nr
Match: KAA0044339.1 (trypsin inhibitor 1-like [Cucumis melo var. makuwa] >TYK29468.1 trypsin inhibitor 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 57.0 bits (136), Expect = 3.99e-08
Identity = 35/79 (44.30%), Postives = 45/79 (56.96%), Query Frame = 0

Query: 18 MIVLLLGTSISANNMVDANAMEVEEGNGIFM---LPRKMM-KNGLLIFQYGVCPLIYMPC 77
          MI++L   S SA+ +VD         +G  M    PRK+M K G+   +  +CP I M C
Sbjct: 14 MIIMLAAMSSSASFVVDDKDSTNLVSDGRDMNNGCPRKIMIKGGVFKEEQRMCPKILMKC 73

Query: 78 KQDSDCLKDCVCKQTGICG 92
          KQDSDCL DCVC + G CG
Sbjct: 74 KQDSDCLLDCVCLKEGFCG 92

BLAST of MELO.jh031516.1 vs. NCBI nr
Match: AAB22436.1 (serine proteinase inhibitor I, trypsin inhibitor I, LLTI-I [Lagenaria leucantha=gourd, Rusby, var. Gourda Makino, seeds, Peptide, 31 aa] [Lagenaria siceraria])

HSP 1 Score: 53.5 bits (127), Expect = 2.39e-07
Identity = 21/28 (75.00%), Postives = 22/28 (78.57%), Query Frame = 0

Query: 66 CPLIYMPCKQDSDCLKDCVCKQTGICGG 93
          CP IYM CK DSDCL DCVC + GICGG
Sbjct: 4  CPRIYMECKHDSDCLADCVCLEHGICGG 31

BLAST of MELO.jh031516.1 vs. NCBI nr
Match: P11969.1 (RecName: Full=Trypsin inhibitor 1; AltName: Full=CVTI-I; AltName: Full=Trypsin inhibitor I [Citrullus lanatus])

HSP 1 Score: 51.6 bits (122), Expect = 1.34e-06
Identity = 20/27 (74.07%), Postives = 22/27 (81.48%), Query Frame = 0

Query: 66 CPLIYMPCKQDSDCLKDCVCKQTGICG 92
          CP IYM CK+D+DCL DCVC Q GICG
Sbjct: 4  CPRIYMECKRDADCLADCVCLQHGICG 30

BLAST of MELO.jh031516.1 vs. ExPASy Swiss-Prot
Match: P11969 (Trypsin inhibitor 1 OS=Citrullus lanatus OX=3654 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 4.4e-06
Identity = 20/27 (74.07%), Postives = 22/27 (81.48%), Query Frame = 0

Query: 66 CPLIYMPCKQDSDCLKDCVCKQTGICG 93
          CP IYM CK+D+DCL DCVC Q GICG
Sbjct: 4  CPRIYMECKRDADCLADCVCLQHGICG 30

BLAST of MELO.jh031516.1 vs. ExPASy Swiss-Prot
Match: P26771 (Trypsin inhibitor 1 OS=Lagenaria siceraria OX=3668 PE=1 SV=2)

HSP 1 Score: 51.6 bits (122), Expect = 5.7e-06
Identity = 20/27 (74.07%), Postives = 21/27 (77.78%), Query Frame = 0

Query: 66 CPLIYMPCKQDSDCLKDCVCKQTGICG 93
          CP IYM CK DSDCL DCVC + GICG
Sbjct: 4  CPRIYMECKHDSDCLADCVCLEHGICG 30

BLAST of MELO.jh031516.1 vs. ExPASy Swiss-Prot
Match: P17680 (Trypsin inhibitor 1 OS=Momordica repens OX=3675 PE=1 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 9.8e-06
Identity = 19/29 (65.52%), Postives = 22/29 (75.86%), Query Frame = 0

Query: 64 GVCPLIYMPCKQDSDCLKDCVCKQTGICG 93
          G+CP I M CK+DSDCL  CVCK+ G CG
Sbjct: 1  GICPRILMECKRDSDCLAQCVCKRQGYCG 29

BLAST of MELO.jh031516.1 vs. ExPASy Swiss-Prot
Match: P34950 (Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 9.8e-06
Identity = 22/44 (50.00%), Postives = 25/44 (56.82%), Query Frame = 0

Query: 49 LPRKMMKNGLLIFQYGVCPLIYMPCKQDSDCLKDCVCKQTGICG 93
          LPRK+M         G+CP I MPCK D DC+ DC C   G CG
Sbjct: 24 LPRKIMSGR----HGGICPRILMPCKTDDDCMLDCRCLSNGYCG 63

BLAST of MELO.jh031516.1 vs. ExPASy Swiss-Prot
Match: P32041 (Trypsin inhibitor 3 OS=Cucumis melo var. conomon OX=3657 PE=1 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 3.7e-05
Identity = 19/28 (67.86%), Postives = 21/28 (75.00%), Query Frame = 0

Query: 65 VCPLIYMPCKQDSDCLKDCVCKQTGICG 93
          +CP I M CKQDSDCL DCVC + G CG
Sbjct: 3  MCPKILMKCKQDSDCLLDCVCLKEGFCG 30

BLAST of MELO.jh031516.1 vs. ExPASy TrEMBL
Match: A0A5D3E148 (Trypsin inhibitor 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00640 PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 1.93e-08
Identity = 35/79 (44.30%), Postives = 45/79 (56.96%), Query Frame = 0

Query: 18 MIVLLLGTSISANNMVDANAMEVEEGNGIFM---LPRKMM-KNGLLIFQYGVCPLIYMPC 77
          MI++L   S SA+ +VD         +G  M    PRK+M K G+   +  +CP I M C
Sbjct: 14 MIIMLAAMSSSASFVVDDKDSTNLVSDGRDMNNGCPRKIMIKGGVFKEEQRMCPKILMKC 73

Query: 78 KQDSDCLKDCVCKQTGICG 92
          KQDSDCL DCVC + G CG
Sbjct: 74 KQDSDCLLDCVCLKEGFCG 92

BLAST of MELO.jh031516.1 vs. ExPASy TrEMBL
Match: Q9S8W3 (CMCTI-I=TRYPSIN inhibitor OS=Cucumis melo OX=3656 PE=3 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 1.03e-05
Identity = 19/28 (67.86%), Postives = 21/28 (75.00%), Query Frame = 0

Query: 65 VCPLIYMPCKQDSDCLKDCVCKQTGICG 92
          +CP I M CKQDSDCL DCVC + G CG
Sbjct: 1  MCPKILMKCKQDSDCLLDCVCLKEGFCG 28

BLAST of MELO.jh031516.1 vs. ExPASy TrEMBL
Match: Q9S8W2 (CMCTI-III=TRYPSIN inhibitor OS=Cucumis melo OX=3656 PE=3 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 1.05e-05
Identity = 19/28 (67.86%), Postives = 21/28 (75.00%), Query Frame = 0

Query: 65 VCPLIYMPCKQDSDCLKDCVCKQTGICG 92
          +CP I M CKQDSDCL DCVC + G CG
Sbjct: 3  MCPKILMKCKQDSDCLLDCVCLKEGFCG 30

BLAST of MELO.jh031516.1 vs. ExPASy TrEMBL
Match: A0A6J1E4H0 (probable polygalacturonase At3g15720 OS=Cucurbita moschata OX=3662 GN=LOC111430533 PE=3 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.85e-05
Identity = 30/67 (44.78%), Postives = 44/67 (65.67%), Query Frame = 0

Query: 20 VLLLGTSISANNMVDANAME-VEEGNGI-FMLPRKMMKNGLLIFQYGVCPLIYMPCKQDS 79
          +LL+ T  SA   +D ++M+ V +G  I   +PRK+MK G+L  +  VCP I M CK+DS
Sbjct: 14 ILLVATLSSAAYAIDDDSMDLVFDGRDINHRVPRKIMKWGVLNHEERVCPKILMECKKDS 73

Query: 80 DCLKDCV 84
          DCL +C+
Sbjct: 74 DCLAECL 80

BLAST of MELO.jh031516.1 vs. ExPASy TrEMBL
Match: A0A6J1JBL8 (trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482991 PE=3 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 1.17e-04
Identity = 23/49 (46.94%), Postives = 29/49 (59.18%), Query Frame = 0

Query: 44 NGIFMLPRKMMKNGLLIFQYGVCPLIYMPCKQDSDCLKDCVCKQTGICG 92
          +G+   PRKMM NG+       CP I M C+ DSDCL  C+C+  G CG
Sbjct: 37 DGVNDFPRKMM-NGV-----QGCPRILMECELDSDCLPGCICRPNGFCG 79

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6576919.19.43e-1045.33hypothetical protein SDJN03_24493, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7014944.19.43e-1045.33hypothetical protein SDJN02_22575, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAA0044339.13.99e-0844.30trypsin inhibitor 1-like [Cucumis melo var. makuwa] >TYK29468.1 trypsin inhibito... [more]
AAB22436.12.39e-0775.00serine proteinase inhibitor I, trypsin inhibitor I, LLTI-I [Lagenaria leucantha=... [more]
P11969.11.34e-0674.07RecName: Full=Trypsin inhibitor 1; AltName: Full=CVTI-I; AltName: Full=Trypsin i... [more]
Match NameE-valueIdentityDescription
P119694.4e-0674.07Trypsin inhibitor 1 OS=Citrullus lanatus OX=3654 PE=1 SV=1[more]
P267715.7e-0674.07Trypsin inhibitor 1 OS=Lagenaria siceraria OX=3668 PE=1 SV=2[more]
P176809.8e-0665.52Trypsin inhibitor 1 OS=Momordica repens OX=3675 PE=1 SV=1[more]
P349509.8e-0650.00Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1[more]
P320413.7e-0567.86Trypsin inhibitor 3 OS=Cucumis melo var. conomon OX=3657 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3E1481.93e-0844.30Trypsin inhibitor 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
Q9S8W31.03e-0567.86CMCTI-I=TRYPSIN inhibitor OS=Cucumis melo OX=3656 PE=3 SV=1[more]
Q9S8W21.05e-0567.86CMCTI-III=TRYPSIN inhibitor OS=Cucumis melo OX=3656 PE=3 SV=1[more]
A0A6J1E4H01.85e-0544.78probable polygalacturonase At3g15720 OS=Cucurbita moschata OX=3662 GN=LOC1114305... [more]
A0A6J1JBL81.17e-0446.94trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482991 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00286PTI_1coord: 64..92
e-value: 2.2E-8
score: 43.9
NoneNo IPR availableGENE3D4.10.75.20coord: 42..92
e-value: 3.2E-12
score: 48.1
IPR000737Proteinase inhibitor I7, squashPFAMPF00299Squashcoord: 65..92
e-value: 3.9E-13
score: 49.2
IPR000737Proteinase inhibitor I7, squashPROSITEPS00286SQUASH_INHIBITORcoord: 66..85
IPR000737Proteinase inhibitor I7, squashCDDcd00150PlantTIcoord: 66..92
e-value: 5.71481E-10
score: 48.1427
IPR011052Proteinase/amylase inhibitor domain superfamilySUPERFAMILY57027Plant inhibitors of proteinases and amylasescoord: 63..92

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO.jh031516.1.t1MELO.jh031516.1.t1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004866 endopeptidase inhibitor activity
molecular_function GO:0004867 serine-type endopeptidase inhibitor activity