MELO.jh008690.1 (gene) Melon (Harukei-3) v1.41

Overview
NameMELO.jh008690.1
Typegene
OrganismCucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
Descriptionpentatricopeptide repeat-containing protein DOT4, chloroplastic-like
Locationchr04: 25628384 .. 25630588 (-)
RNA-Seq ExpressionMELO.jh008690.1
SyntenyMELO.jh008690.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidestart_codonstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
CATTTAAAGAATGAATGACAATGGAAAGTTAAGTTCTCCTCTTTTGTTGTCCTCTGTTAGTGTTTCCCGATGCCTAACGTCTAAAACTCTATCATGAATCTTCTTCTCTCCACCCACACCCACTGTCTCCCCATTACCCAAAAACCCTATCACGCATACCATCGCCACCCACCATTTAATAATCTCCCTCATGTTCGTACTACGACTGTCGAGAATTATGCTGATTTATGCGTAGCCCACCAAGTGTTCGACGAAATTCCTATTTGGGATACTTTTGCATGGAACAATCTGATTCAAACCCATCTCACCAATGGAGATTGGGGGCATGTAATTTCAATTTATCGACAGATGTTATTTCGTGGAGTTCGTCCTGACAAACACACCCTTCCTCGAATTATATGTGCTACACGCCAGTATGGCGATCTGCCGGTTGGCAAACAGCTCCACGCTCAAGCCTTCAAACTTGGGTTCTCCTCTGACCTTTATGTACTTACTTCCTTAATTGAGTTATATGGGATTCTTGACAGTGCTGACACTGCAAAGTGGCTTCATGACAAGTCCACTTGTAGAAACTCTGTTTCTTGGACCATTTTAGCTAAGCTGTACTTGAGGGAAGATAAACCCAGTTTTGCTATAGACTTGTTTTACCAAATGGTGGAGTTGGCGGATGATATTGATTCAGTGGCTTTGGCCACGGCCATTGGTGCCTGTGGTGCTCTCAAAATGCTGCATCATGGAAGAAACATCCACCATCTCGCAAGAATTCATGGCTTGGAATTTAATATATTGGTCAGTAATTCTCTATTGAAAATGTACCTCGACTGTGATAGTATCAAAGATGCTCGTGGGTTCTTCGACCAAATGCCGTCCAAAGATGTAATTTCCTGGACAGAACTTATCCATATGTACGTTAAGAAAGGTGGAATCAATGAGGCCTTTAAGCTGTTTCGACAAATGAATATGGATGGAGAATTGAAGCCTGATCCTCTTACAATCAGCAGCATTCTCCCAGCTTGTGGAAGAATGGCTGCACATAAGCATGGAAAAGAAATTCATGGATATGTGCTTAAAAATGGTTTTGACGAGAATCTCATTGTCCAAAATGCTTTGGTCGACATGTATGTCAAATCTGGATGTATCCAGTCTGCATCAAAAACTTTCTCGATGATGAAGGAGAAAGATATGGTTTCGTGGAGCATCATGACTTTGGGCTACAGCTTACACGGTCAAGGAAAACTTGGAGTCGGTTTGTTCCGTGAGATGGAGAAGAACTTGAAGATGCATAGAGATGAGATCACTTACACTGCAGTTTTGCATGCTTGTACTACTGCAAACATGGTAGATGAAGGGGATTTTTACTTCAGTCGCATTACCAAACCAACCGTGGCACACATTGCTCTCAAGGTGGCTCTTTTAGCTCGAGCGGGGAGACTGGATGAAGCTAGGACATTTGTAGAAAAGAAGAAACTTAATAAACATCCTGAGATTTTGAGAGCATTGCTCGATGGATGCAGGAACCACCGTCAACAAAAACTAGGCAAACGAATCATTGAGCAGCTGTGTGATTTGGAGCCTCTAAATACTGAGAATTACATTCTACTTTCGAACTGGTATGCGTGCAACAAAAAATGGGACATGGTTGAAGAGTTGAGAGAAACAATAAGAGACATGGGATTAAGACCCAAGAAGGCTTACAGTTGGATAGAGTTCTGCAACAAAATTCACGTGTTCGGGACAGGGGATGTATCCCACCCGAGATCACAAAACATATATTGGAATTTACAGTGCTTAATGAAAAAAATGGAAGAAGATGGTTCCAAGACGAATCCTGAATTCAGCTTGCACGACGTCGACGAGGAGCGAGAGTGTGTTCCAATAGGACACAGCGAATTGTTGGCGATTTCATTCGGGCTGATTAGTACAGAAGCAGGAAGGACAATTCGTATTACAAAGAACCTTCGTGTATGTCATAGTTGTCATGAGTCTGCAAAGTTTATATCCAAGATGGTTGGCCGAGAAATCATAGTAAAAGATCCTTATGTTTTCCATCATTTTAAAGATGGCTGCTGTTCTTGTGAAAACTTTTGTTAGCTTTATGGATGGTCGTCGTTGGTTATAATTTAGAGACCTCCACTGCTCTCATTTGGGTGTAGCTTAGTTCTATAACGTTATGAGATTTAAAAATAAACAAATTTTCAGGAGAAA

mRNA sequence

CATTTAAAGAATGAATGACAATGGAAAGTTAAGTTCTCCTCTTTTGTTGTCCTCTGTTAGTGTTTCCCGATGCCTAACGTCTAAAACTCTATCATGAATCTTCTTCTCTCCACCCACACCCACTGTCTCCCCATTACCCAAAAACCCTATCACGCATACCATCGCCACCCACCATTTAATAATCTCCCTCATGTTCGTACTACGACTGTCGAGAATTATGCTGATTTATGCGTAGCCCACCAAGTGTTCGACGAAATTCCTATTTGGGATACTTTTGCATGGAACAATCTGATTCAAACCCATCTCACCAATGGAGATTGGGGGCATGTAATTTCAATTTATCGACAGATGTTATTTCGTGGAGTTCGTCCTGACAAACACACCCTTCCTCGAATTATATGTGCTACACGCCAGTATGGCGATCTGCCGGTTGGCAAACAGCTCCACGCTCAAGCCTTCAAACTTGGGTTCTCCTCTGACCTTTATGTACTTACTTCCTTAATTGAGTTATATGGGATTCTTGACAGTGCTGACACTGCAAAGTGGCTTCATGACAAGTCCACTTGTAGAAACTCTGTTTCTTGGACCATTTTAGCTAAGCTGTACTTGAGGGAAGATAAACCCAGTTTTGCTATAGACTTGTTTTACCAAATGGTGGAGTTGGCGGATGATATTGATTCAGTGGCTTTGGCCACGGCCATTGGTGCCTGTGGTGCTCTCAAAATGCTGCATCATGGAAGAAACATCCACCATCTCGCAAGAATTCATGGCTTGGAATTTAATATATTGGTCAGTAATTCTCTATTGAAAATGTACCTCGACTGTGATAGTATCAAAGATGCTCGTGGGTTCTTCGACCAAATGCCGTCCAAAGATGTAATTTCCTGGACAGAACTTATCCATATGTACGTTAAGAAAGGTGGAATCAATGAGGCCTTTAAGCTGTTTCGACAAATGAATATGGATGGAGAATTGAAGCCTGATCCTCTTACAATCAGCAGCATTCTCCCAGCTTGTGGAAGAATGGCTGCACATAAGCATGGAAAAGAAATTCATGGATATGTGCTTAAAAATGGTTTTGACGAGAATCTCATTGTCCAAAATGCTTTGGTCGACATGTATGTCAAATCTGGATGTATCCAGTCTGCATCAAAAACTTTCTCGATGATGAAGGAGAAAGATATGGTTTCGTGGAGCATCATGACTTTGGGCTACAGCTTACACGGTCAAGGAAAACTTGGAGTCGGTTTGTTCCGTGAGATGGAGAAGAACTTGAAGATGCATAGAGATGAGATCACTTACACTGCAGTTTTGCATGCTTGTACTACTGCAAACATGGTAGATGAAGGGGATTTTTACTTCAGTCGCATTACCAAACCAACCGTGGCACACATTGCTCTCAAGGTGGCTCTTTTAGCTCGAGCGGGGAGACTGGATGAAGCTAGGACATTTGTAGAAAAGAAGAAACTTAATAAACATCCTGAGATTTTGAGAGCATTGCTCGATGGATGCAGGAACCACCGTCAACAAAAACTAGGCAAACGAATCATTGAGCAGCTGTGTGATTTGGAGCCTCTAAATACTGAGAATTACATTCTACTTTCGAACTGGTATGCGTGCAACAAAAAATGGGACATGGTTGAAGAGTTGAGAGAAACAATAAGAGACATGGGATTAAGACCCAAGAAGGCTTACAGTTGGATAGAGTTCTGCAACAAAATTCACGTGTTCGGGACAGGGGATGTATCCCACCCGAGATCACAAAACATATATTGGAATTTACAGTGCTTAATGAAAAAAATGGAAGAAGATGGTTCCAAGACGAATCCTGAATTCAGCTTGCACGACGTCGACGAGGAGCGAGAGTGTGTTCCAATAGGACACAGCGAATTGTTGGCGATTTCATTCGGGCTGATTAGTACAGAAGCAGGAAGGACAATTCGTATTACAAAGAACCTTCGTGTATGTCATAGTTGTCATGAGTCTGCAAAGTTTATATCCAAGATGGTTGGCCGAGAAATCATAGTAAAAGATCCTTATGTTTTCCATCATTTTAAAGATGGCTGCTGTTCTTGTGAAAACTTTTGTTAGCTTTATGGATGGTCGTCGTTGGTTATAATTTAGAGACCTCCACTGCTCTCATTTGGGTGTAGCTTAGTTCTATAACGTTATGAGATTTAAAAATAAACAAATTTTCAGGAGAAA

Coding sequence (CDS)

ATGAATCTTCTTCTCTCCACCCACACCCACTGTCTCCCCATTACCCAAAAACCCTATCACGCATACCATCGCCACCCACCATTTAATAATCTCCCTCATGTTCGTACTACGACTGTCGAGAATTATGCTGATTTATGCGTAGCCCACCAAGTGTTCGACGAAATTCCTATTTGGGATACTTTTGCATGGAACAATCTGATTCAAACCCATCTCACCAATGGAGATTGGGGGCATGTAATTTCAATTTATCGACAGATGTTATTTCGTGGAGTTCGTCCTGACAAACACACCCTTCCTCGAATTATATGTGCTACACGCCAGTATGGCGATCTGCCGGTTGGCAAACAGCTCCACGCTCAAGCCTTCAAACTTGGGTTCTCCTCTGACCTTTATGTACTTACTTCCTTAATTGAGTTATATGGGATTCTTGACAGTGCTGACACTGCAAAGTGGCTTCATGACAAGTCCACTTGTAGAAACTCTGTTTCTTGGACCATTTTAGCTAAGCTGTACTTGAGGGAAGATAAACCCAGTTTTGCTATAGACTTGTTTTACCAAATGGTGGAGTTGGCGGATGATATTGATTCAGTGGCTTTGGCCACGGCCATTGGTGCCTGTGGTGCTCTCAAAATGCTGCATCATGGAAGAAACATCCACCATCTCGCAAGAATTCATGGCTTGGAATTTAATATATTGGTCAGTAATTCTCTATTGAAAATGTACCTCGACTGTGATAGTATCAAAGATGCTCGTGGGTTCTTCGACCAAATGCCGTCCAAAGATGTAATTTCCTGGACAGAACTTATCCATATGTACGTTAAGAAAGGTGGAATCAATGAGGCCTTTAAGCTGTTTCGACAAATGAATATGGATGGAGAATTGAAGCCTGATCCTCTTACAATCAGCAGCATTCTCCCAGCTTGTGGAAGAATGGCTGCACATAAGCATGGAAAAGAAATTCATGGATATGTGCTTAAAAATGGTTTTGACGAGAATCTCATTGTCCAAAATGCTTTGGTCGACATGTATGTCAAATCTGGATGTATCCAGTCTGCATCAAAAACTTTCTCGATGATGAAGGAGAAAGATATGGTTTCGTGGAGCATCATGACTTTGGGCTACAGCTTACACGGTCAAGGAAAACTTGGAGTCGGTTTGTTCCGTGAGATGGAGAAGAACTTGAAGATGCATAGAGATGAGATCACTTACACTGCAGTTTTGCATGCTTGTACTACTGCAAACATGGTAGATGAAGGGGATTTTTACTTCAGTCGCATTACCAAACCAACCGTGGCACACATTGCTCTCAAGGTGGCTCTTTTAGCTCGAGCGGGGAGACTGGATGAAGCTAGGACATTTGTAGAAAAGAAGAAACTTAATAAACATCCTGAGATTTTGAGAGCATTGCTCGATGGATGCAGGAACCACCGTCAACAAAAACTAGGCAAACGAATCATTGAGCAGCTGTGTGATTTGGAGCCTCTAAATACTGAGAATTACATTCTACTTTCGAACTGGTATGCGTGCAACAAAAAATGGGACATGGTTGAAGAGTTGAGAGAAACAATAAGAGACATGGGATTAAGACCCAAGAAGGCTTACAGTTGGATAGAGTTCTGCAACAAAATTCACGTGTTCGGGACAGGGGATGTATCCCACCCGAGATCACAAAACATATATTGGAATTTACAGTGCTTAATGAAAAAAATGGAAGAAGATGGTTCCAAGACGAATCCTGAATTCAGCTTGCACGACGTCGACGAGGAGCGAGAGTGTGTTCCAATAGGACACAGCGAATTGTTGGCGATTTCATTCGGGCTGATTAGTACAGAAGCAGGAAGGACAATTCGTATTACAAAGAACCTTCGTGTATGTCATAGTTGTCATGAGTCTGCAAAGTTTATATCCAAGATGGTTGGCCGAGAAATCATAGTAAAAGATCCTTATGTTTTCCATCATTTTAAAGATGGCTGCTGTTCTTGTGAAAACTTTTGTTAG

Protein sequence

MNLLLSTHTHCLPITQKPYHAYHRHPPFNNLPHVRTTTVENYADLCVAHQVFDEIPIWDTFAWNNLIQTHLTNGDWGHVISIYRQMLFRGVRPDKHTLPRIICATRQYGDLPVGKQLHAQAFKLGFSSDLYVLTSLIELYGILDSADTAKWLHDKSTCRNSVSWTILAKLYLREDKPSFAIDLFYQMVELADDIDSVALATAIGACGALKMLHHGRNIHHLARIHGLEFNILVSNSLLKMYLDCDSIKDARGFFDQMPSKDVISWTELIHMYVKKGGINEAFKLFRQMNMDGELKPDPLTISSILPACGRMAAHKHGKEIHGYVLKNGFDENLIVQNALVDMYVKSGCIQSASKTFSMMKEKDMVSWSIMTLGYSLHGQGKLGVGLFREMEKNLKMHRDEITYTAVLHACTTANMVDEGDFYFSRITKPTVAHIALKVALLARAGRLDEARTFVEKKKLNKHPEILRALLDGCRNHRQQKLGKRIIEQLCDLEPLNTENYILLSNWYACNKKWDMVEELRETIRDMGLRPKKAYSWIEFCNKIHVFGTGDVSHPRSQNIYWNLQCLMKKMEEDGSKTNPEFSLHDVDEERECVPIGHSELLAISFGLISTEAGRTIRITKNLRVCHSCHESAKFISKMVGREIIVKDPYVFHHFKDGCCSCENFC
Homology
BLAST of MELO.jh008690.1 vs. NCBI nr
Match: XP_008465161.1 (PREDICTED: pentatricopeptide repeat-containing protein DOT4, chloroplastic-like [Cucumis melo])

HSP 1 Score: 1384 bits (3581), Expect = 0.0
Identity = 665/665 (100.00%), Postives = 665/665 (100.00%), Query Frame = 0

Query: 1   MNLLLSTHTHCLPITQKPYHAYHRHPPFNNLPHVRTTTVENYADLCVAHQVFDEIPIWDT 60
           MNLLLSTHTHCLPITQKPYHAYHRHPPFNNLPHVRTTTVENYADLCVAHQVFDEIPIWDT
Sbjct: 1   MNLLLSTHTHCLPITQKPYHAYHRHPPFNNLPHVRTTTVENYADLCVAHQVFDEIPIWDT 60

Query: 61  FAWNNLIQTHLTNGDWGHVISIYRQMLFRGVRPDKHTLPRIICATRQYGDLPVGKQLHAQ 120
           FAWNNLIQTHLTNGDWGHVISIYRQMLFRGVRPDKHTLPRIICATRQYGDLPVGKQLHAQ
Sbjct: 61  FAWNNLIQTHLTNGDWGHVISIYRQMLFRGVRPDKHTLPRIICATRQYGDLPVGKQLHAQ 120

Query: 121 AFKLGFSSDLYVLTSLIELYGILDSADTAKWLHDKSTCRNSVSWTILAKLYLREDKPSFA 180
           AFKLGFSSDLYVLTSLIELYGILDSADTAKWLHDKSTCRNSVSWTILAKLYLREDKPSFA
Sbjct: 121 AFKLGFSSDLYVLTSLIELYGILDSADTAKWLHDKSTCRNSVSWTILAKLYLREDKPSFA 180

Query: 181 IDLFYQMVELADDIDSVALATAIGACGALKMLHHGRNIHHLARIHGLEFNILVSNSLLKM 240
           IDLFYQMVELADDIDSVALATAIGACGALKMLHHGRNIHHLARIHGLEFNILVSNSLLKM
Sbjct: 181 IDLFYQMVELADDIDSVALATAIGACGALKMLHHGRNIHHLARIHGLEFNILVSNSLLKM 240

Query: 241 YLDCDSIKDARGFFDQMPSKDVISWTELIHMYVKKGGINEAFKLFRQMNMDGELKPDPLT 300
           YLDCDSIKDARGFFDQMPSKDVISWTELIHMYVKKGGINEAFKLFRQMNMDGELKPDPLT
Sbjct: 241 YLDCDSIKDARGFFDQMPSKDVISWTELIHMYVKKGGINEAFKLFRQMNMDGELKPDPLT 300

Query: 301 ISSILPACGRMAAHKHGKEIHGYVLKNGFDENLIVQNALVDMYVKSGCIQSASKTFSMMK 360
           ISSILPACGRMAAHKHGKEIHGYVLKNGFDENLIVQNALVDMYVKSGCIQSASKTFSMMK
Sbjct: 301 ISSILPACGRMAAHKHGKEIHGYVLKNGFDENLIVQNALVDMYVKSGCIQSASKTFSMMK 360

Query: 361 EKDMVSWSIMTLGYSLHGQGKLGVGLFREMEKNLKMHRDEITYTAVLHACTTANMVDEGD 420
           EKDMVSWSIMTLGYSLHGQGKLGVGLFREMEKNLKMHRDEITYTAVLHACTTANMVDEGD
Sbjct: 361 EKDMVSWSIMTLGYSLHGQGKLGVGLFREMEKNLKMHRDEITYTAVLHACTTANMVDEGD 420

Query: 421 FYFSRITKPTVAHIALKVALLARAGRLDEARTFVEKKKLNKHPEILRALLDGCRNHRQQK 480
           FYFSRITKPTVAHIALKVALLARAGRLDEARTFVEKKKLNKHPEILRALLDGCRNHRQQK
Sbjct: 421 FYFSRITKPTVAHIALKVALLARAGRLDEARTFVEKKKLNKHPEILRALLDGCRNHRQQK 480

Query: 481 LGKRIIEQLCDLEPLNTENYILLSNWYACNKKWDMVEELRETIRDMGLRPKKAYSWIEFC 540
           LGKRIIEQLCDLEPLNTENYILLSNWYACNKKWDMVEELRETIRDMGLRPKKAYSWIEFC
Sbjct: 481 LGKRIIEQLCDLEPLNTENYILLSNWYACNKKWDMVEELRETIRDMGLRPKKAYSWIEFC 540

Query: 541 NKIHVFGTGDVSHPRSQNIYWNLQCLMKKMEEDGSKTNPEFSLHDVDEERECVPIGHSEL 600
           NKIHVFGTGDVSHPRSQNIYWNLQCLMKKMEEDGSKTNPEFSLHDVDEERECVPIGHSEL
Sbjct: 541 NKIHVFGTGDVSHPRSQNIYWNLQCLMKKMEEDGSKTNPEFSLHDVDEERECVPIGHSEL 600

Query: 601 LAISFGLISTEAGRTIRITKNLRVCHSCHESAKFISKMVGREIIVKDPYVFHHFKDGCCS 660
           LAISFGLISTEAGRTIRITKNLRVCHSCHESAKFISKMVGREIIVKDPYVFHHFKDGCCS
Sbjct: 601 LAISFGLISTEAGRTIRITKNLRVCHSCHESAKFISKMVGREIIVKDPYVFHHFKDGCCS 660

Query: 661 CENFC 665
           CENFC
Sbjct: 661 CENFC 665

BLAST of MELO.jh008690.1 vs. NCBI nr
Match: XP_004137884.2 (pentatricopeptide repeat-containing protein DOT4, chloroplastic [Cucumis sativus])

HSP 1 Score: 1326 bits (3431), Expect = 0.0
Identity = 635/665 (95.49%), Postives = 650/665 (97.74%), Query Frame = 0

Query: 1   MNLLLSTHTHCLPITQKPYHAYHRHPPFNNLPHVRTTTVENYADLCVAHQVFDEIPIWDT 60
           MNLLLSTHTHCLPITQKP HAYHRHPPFNNLPHVRT TVENYA+LCVAHQVFD+IPIWDT
Sbjct: 1   MNLLLSTHTHCLPITQKPNHAYHRHPPFNNLPHVRTMTVENYANLCVAHQVFDDIPIWDT 60

Query: 61  FAWNNLIQTHLTNGDWGHVISIYRQMLFRGVRPDKHTLPRIICATRQYGDLPVGKQLHAQ 120
           FAWNNLIQTHLTNGD GHVIS YRQMLFRGVRPDKHTLPRIICATRQYGDL VGKQLHAQ
Sbjct: 61  FAWNNLIQTHLTNGDLGHVISTYRQMLFRGVRPDKHTLPRIICATRQYGDLQVGKQLHAQ 120

Query: 121 AFKLGFSSDLYVLTSLIELYGILDSADTAKWLHDKSTCRNSVSWTILAKLYLREDKPSFA 180
           AFKLGFSS+LYVLTSLIELYGILDSADTAKWLHDKSTCRNSVSWT+LAKLYLREDKPS A
Sbjct: 121 AFKLGFSSNLYVLTSLIELYGILDSADTAKWLHDKSTCRNSVSWTVLAKLYLREDKPSLA 180

Query: 181 IDLFYQMVELADDIDSVALATAIGACGALKMLHHGRNIHHLARIHGLEFNILVSNSLLKM 240
           +DLFYQMVELADDID+VALATAIGACGALKMLHHGRNIHHLAR+HGLEFNILVSNSLLKM
Sbjct: 181 LDLFYQMVELADDIDAVALATAIGACGALKMLHHGRNIHHLARVHGLEFNILVSNSLLKM 240

Query: 241 YLDCDSIKDARGFFDQMPSKDVISWTELIHMYVKKGGINEAFKLFRQMNMDGELKPDPLT 300
           Y+DCDSIKDARGFFDQMPSKD+ISWTELIHMYVKKGGINEAFKLFRQMNMDGELKPDP T
Sbjct: 241 YIDCDSIKDARGFFDQMPSKDIISWTELIHMYVKKGGINEAFKLFRQMNMDGELKPDPRT 300

Query: 301 ISSILPACGRMAAHKHGKEIHGYVLKNGFDENLIVQNALVDMYVKSGCIQSASKTFSMMK 360
           ISSILPACGRMAAHKHGKEIHGYV+KN FDENLIVQNALVDMYVKSGCIQSASKTFSMMK
Sbjct: 301 ISSILPACGRMAAHKHGKEIHGYVVKNAFDENLIVQNALVDMYVKSGCIQSASKTFSMMK 360

Query: 361 EKDMVSWSIMTLGYSLHGQGKLGVGLFREMEKNLKMHRDEITYTAVLHACTTANMVDEGD 420
           EKDMVSWSIMTLGYSLHGQGKLGV LFREMEKN KM RDEITYTAVLHACTTANMVDEGD
Sbjct: 361 EKDMVSWSIMTLGYSLHGQGKLGVSLFREMEKNFKMRRDEITYTAVLHACTTANMVDEGD 420

Query: 421 FYFSRITKPTVAHIALKVALLARAGRLDEARTFVEKKKLNKHPEILRALLDGCRNHRQQK 480
            YFS ITKPTVAHIALKVALLARAGRLDEARTFVEKKKL+KHPEILRALLDGCRNHRQQK
Sbjct: 421 SYFSCITKPTVAHIALKVALLARAGRLDEARTFVEKKKLDKHPEILRALLDGCRNHRQQK 480

Query: 481 LGKRIIEQLCDLEPLNTENYILLSNWYACNKKWDMVEELRETIRDMGLRPKKAYSWIEFC 540
           LGKRIIEQLCDLEPLN ENYILLSNWYACN+KWDMVE+LRETIRDMGLRPKKAYSWIEFC
Sbjct: 481 LGKRIIEQLCDLEPLNAENYILLSNWYACNEKWDMVEKLRETIRDMGLRPKKAYSWIEFC 540

Query: 541 NKIHVFGTGDVSHPRSQNIYWNLQCLMKKMEEDGSKTNPEFSLHDVDEERECVPIGHSEL 600
           NKIHVFGTGDVSHPRSQNIYWNLQCLMK+MEEDGSK NP+FSLHDVDEERECVPIGHSEL
Sbjct: 541 NKIHVFGTGDVSHPRSQNIYWNLQCLMKEMEEDGSKPNPDFSLHDVDEERECVPIGHSEL 600

Query: 601 LAISFGLISTEAGRTIRITKNLRVCHSCHESAKFISKMVGREIIVKDPYVFHHFKDGCCS 660
           LAISFGLISTEAGRTIRITKNLRVCHSCHESAKFISKMVGREIIVKDPYVFHHFKDGCCS
Sbjct: 601 LAISFGLISTEAGRTIRITKNLRVCHSCHESAKFISKMVGREIIVKDPYVFHHFKDGCCS 660

Query: 661 CENFC 665
           CENFC
Sbjct: 661 CENFC 665

BLAST of MELO.jh008690.1 vs. NCBI nr
Match: XP_038905218.1 (pentatricopeptide repeat-containing protein DOT4, chloroplastic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1233 bits (3191), Expect = 0.0
Identity = 598/666 (89.79%), Postives = 627/666 (94.14%), Query Frame = 0

Query: 1   MNLLLSTHTHCLPITQKPYHAYHRHPPFNNLPHVRTTTVENYADLCVAHQVFDEIPIWDT 60
           MNLLLSTH HCLPITQ+  H      PFNN PHVRTTT +N A+L VAHQ+FDEIPIWDT
Sbjct: 1   MNLLLSTHVHCLPITQETSHR----QPFNNPPHVRTTTAKNSANLSVAHQLFDEIPIWDT 60

Query: 61  FAWNNLIQTHLTNGDWGHVISIYRQMLFRGVRPDKHTLPRIICATRQYGDLPVGKQLHAQ 120
           FAWNNLIQTHLTNGD GHVIS Y+QMLFRGVRPDKHTLPRIICATRQYG+L  GKQLHAQ
Sbjct: 61  FAWNNLIQTHLTNGDLGHVISTYQQMLFRGVRPDKHTLPRIICATRQYGNLQFGKQLHAQ 120

Query: 121 AFKLGFSSDLYVLTSLIELYGILDSADTAKWLHDKSTCRNSVSWTILAKLYLREDKPSFA 180
           AFKLGFSS+LYVLTSLIE YGILDSADTAKWLHDKS CRNSVSWT+LAKLYL EDKPS A
Sbjct: 121 AFKLGFSSNLYVLTSLIEFYGILDSADTAKWLHDKSACRNSVSWTMLAKLYLMEDKPSCA 180

Query: 181 IDLFYQMVELADDIDSVALATAIGACGALKMLHHGRNIHHLARIHGLEFNILVSNSLLKM 240
           IDLFYQMVELADDID+VALATAIGACGALKML HGRNIH LARIHGLEFN+LVSNSLLKM
Sbjct: 181 IDLFYQMVELADDIDAVALATAIGACGALKMLQHGRNIHLLARIHGLEFNVLVSNSLLKM 240

Query: 241 YLDCDSIKDARGFFDQMPSKDVISWTELIHMYVKKGGINEAFKLFRQMNMDGELKPDPLT 300
           YLDCDSIKDARGFFD+MPSKDVISWTELIHMYVKKGGINEAFKLFRQMN DG LKPDPLT
Sbjct: 241 YLDCDSIKDARGFFDRMPSKDVISWTELIHMYVKKGGINEAFKLFRQMNKDGGLKPDPLT 300

Query: 301 ISSILPACGRMAAHKHGKEIHGYVLKNGFDENLIVQNALVDMYVKSGCIQSASKTFSMMK 360
           ISSILPACGRMAAHKHGKEIHGYVLKN FDENLIVQNALVDMYVKSGCIQSAS+TFSMMK
Sbjct: 301 ISSILPACGRMAAHKHGKEIHGYVLKNAFDENLIVQNALVDMYVKSGCIQSASETFSMMK 360

Query: 361 EKDMVSWSIMTLGYSLHGQGKLGVGLFREMEKNLKMH-RDEITYTAVLHACTTANMVDEG 420
           EKDMVSW+IMTLGYSLHGQGKLGV LFRE+E+NL+MH RD+ITYTAVLHACTTANMVDEG
Sbjct: 361 EKDMVSWTIMTLGYSLHGQGKLGVSLFREIERNLRMHNRDQITYTAVLHACTTANMVDEG 420

Query: 421 DFYFSRITKPTVAHIALKVALLARAGRLDEARTFVEKKKLNKHPEILRALLDGCRNHRQQ 480
           DFYFS IT+PTVAHIALKVALLARAGRLDEA TFVEK KL+KH  ILRALLDGCR H Q+
Sbjct: 421 DFYFSCITEPTVAHIALKVALLARAGRLDEATTFVEKNKLDKHAVILRALLDGCRKHHQR 480

Query: 481 KLGKRIIEQLCDLEPLNTENYILLSNWYACNKKWDMVEELRETIRDMGLRPKKAYSWIEF 540
           KLGK+IIE+LCDLEPLN ENYILLSNWYACNKKWDMVE+LRET+RDMGLRPKKAYSW+EF
Sbjct: 481 KLGKQIIEKLCDLEPLNAENYILLSNWYACNKKWDMVEKLRETMRDMGLRPKKAYSWMEF 540

Query: 541 CNKIHVFGTGDVSHPRSQNIYWNLQCLMKKMEEDGSKTNPEFSLHDVDEERECVPIGHSE 600
           CNKIHVFGTGDVSHPRS+NIYWNLQCLMKKMEEDGSK NP+FS HDVDEERECVPIGHSE
Sbjct: 541 CNKIHVFGTGDVSHPRSRNIYWNLQCLMKKMEEDGSKPNPDFSFHDVDEERECVPIGHSE 600

Query: 601 LLAISFGLISTEAGRTIRITKNLRVCHSCHESAKFISKMVGREIIVKDPYVFHHFKDGCC 660
           LLAISFGLIST+AGRTIRITKNLRVCHSCHESAKFISKMVGREIIVKDPYVFHHFKDGCC
Sbjct: 601 LLAISFGLISTKAGRTIRITKNLRVCHSCHESAKFISKMVGREIIVKDPYVFHHFKDGCC 660

Query: 661 SCENFC 665
           SCE+ C
Sbjct: 661 SCEDVC 662

BLAST of MELO.jh008690.1 vs. NCBI nr
Match: KAA0037696.1 (pentatricopeptide repeat-containing protein DOT4 [Cucumis melo var. makuwa])

HSP 1 Score: 1196 bits (3095), Expect = 0.0
Identity = 580/580 (100.00%), Postives = 580/580 (100.00%), Query Frame = 0

Query: 86  MLFRGVRPDKHTLPRIICATRQYGDLPVGKQLHAQAFKLGFSSDLYVLTSLIELYGILDS 145
           MLFRGVRPDKHTLPRIICATRQYGDLPVGKQLHAQAFKLGFSSDLYVLTSLIELYGILDS
Sbjct: 1   MLFRGVRPDKHTLPRIICATRQYGDLPVGKQLHAQAFKLGFSSDLYVLTSLIELYGILDS 60

Query: 146 ADTAKWLHDKSTCRNSVSWTILAKLYLREDKPSFAIDLFYQMVELADDIDSVALATAIGA 205
           ADTAKWLHDKSTCRNSVSWTILAKLYLREDKPSFAIDLFYQMVELADDIDSVALATAIGA
Sbjct: 61  ADTAKWLHDKSTCRNSVSWTILAKLYLREDKPSFAIDLFYQMVELADDIDSVALATAIGA 120

Query: 206 CGALKMLHHGRNIHHLARIHGLEFNILVSNSLLKMYLDCDSIKDARGFFDQMPSKDVISW 265
           CGALKMLHHGRNIHHLARIHGLEFNILVSNSLLKMYLDCDSIKDARGFFDQMPSKDVISW
Sbjct: 121 CGALKMLHHGRNIHHLARIHGLEFNILVSNSLLKMYLDCDSIKDARGFFDQMPSKDVISW 180

Query: 266 TELIHMYVKKGGINEAFKLFRQMNMDGELKPDPLTISSILPACGRMAAHKHGKEIHGYVL 325
           TELIHMYVKKGGINEAFKLFRQMNMDGELKPDPLTISSILPACGRMAAHKHGKEIHGYVL
Sbjct: 181 TELIHMYVKKGGINEAFKLFRQMNMDGELKPDPLTISSILPACGRMAAHKHGKEIHGYVL 240

Query: 326 KNGFDENLIVQNALVDMYVKSGCIQSASKTFSMMKEKDMVSWSIMTLGYSLHGQGKLGVG 385
           KNGFDENLIVQNALVDMYVKSGCIQSASKTFSMMKEKDMVSWSIMTLGYSLHGQGKLGVG
Sbjct: 241 KNGFDENLIVQNALVDMYVKSGCIQSASKTFSMMKEKDMVSWSIMTLGYSLHGQGKLGVG 300

Query: 386 LFREMEKNLKMHRDEITYTAVLHACTTANMVDEGDFYFSRITKPTVAHIALKVALLARAG 445
           LFREMEKNLKMHRDEITYTAVLHACTTANMVDEGDFYFSRITKPTVAHIALKVALLARAG
Sbjct: 301 LFREMEKNLKMHRDEITYTAVLHACTTANMVDEGDFYFSRITKPTVAHIALKVALLARAG 360

Query: 446 RLDEARTFVEKKKLNKHPEILRALLDGCRNHRQQKLGKRIIEQLCDLEPLNTENYILLSN 505
           RLDEARTFVEKKKLNKHPEILRALLDGCRNHRQQKLGKRIIEQLCDLEPLNTENYILLSN
Sbjct: 361 RLDEARTFVEKKKLNKHPEILRALLDGCRNHRQQKLGKRIIEQLCDLEPLNTENYILLSN 420

Query: 506 WYACNKKWDMVEELRETIRDMGLRPKKAYSWIEFCNKIHVFGTGDVSHPRSQNIYWNLQC 565
           WYACNKKWDMVEELRETIRDMGLRPKKAYSWIEFCNKIHVFGTGDVSHPRSQNIYWNLQC
Sbjct: 421 WYACNKKWDMVEELRETIRDMGLRPKKAYSWIEFCNKIHVFGTGDVSHPRSQNIYWNLQC 480

Query: 566 LMKKMEEDGSKTNPEFSLHDVDEERECVPIGHSELLAISFGLISTEAGRTIRITKNLRVC 625
           LMKKMEEDGSKTNPEFSLHDVDEERECVPIGHSELLAISFGLISTEAGRTIRITKNLRVC
Sbjct: 481 LMKKMEEDGSKTNPEFSLHDVDEERECVPIGHSELLAISFGLISTEAGRTIRITKNLRVC 540

Query: 626 HSCHESAKFISKMVGREIIVKDPYVFHHFKDGCCSCENFC 665
           HSCHESAKFISKMVGREIIVKDPYVFHHFKDGCCSCENFC
Sbjct: 541 HSCHESAKFISKMVGREIIVKDPYVFHHFKDGCCSCENFC 580

BLAST of MELO.jh008690.1 vs. NCBI nr
Match: TYK10677.1 (pentatricopeptide repeat-containing protein DOT4 [Cucumis melo var. makuwa])

HSP 1 Score: 1193 bits (3086), Expect = 0.0
Identity = 579/580 (99.83%), Postives = 579/580 (99.83%), Query Frame = 0

Query: 86  MLFRGVRPDKHTLPRIICATRQYGDLPVGKQLHAQAFKLGFSSDLYVLTSLIELYGILDS 145
           MLFRGVRPDKHTLPRIICATRQYGDLPVGKQLHAQAFKLGFSSDLYVLTSLIELYGILDS
Sbjct: 1   MLFRGVRPDKHTLPRIICATRQYGDLPVGKQLHAQAFKLGFSSDLYVLTSLIELYGILDS 60

Query: 146 ADTAKWLHDKSTCRNSVSWTILAKLYLREDKPSFAIDLFYQMVELADDIDSVALATAIGA 205
           ADTAKWLHDKSTCRNSVSWTILAKLYLREDKPSFAIDLFYQMVELADDIDSVALATAIGA
Sbjct: 61  ADTAKWLHDKSTCRNSVSWTILAKLYLREDKPSFAIDLFYQMVELADDIDSVALATAIGA 120

Query: 206 CGALKMLHHGRNIHHLARIHGLEFNILVSNSLLKMYLDCDSIKDARGFFDQMPSKDVISW 265
           CGALKMLHHGRNIHHLARIHGLEFNILVSNSLLKMYLDCDSIKDARGFFDQMPSKDVISW
Sbjct: 121 CGALKMLHHGRNIHHLARIHGLEFNILVSNSLLKMYLDCDSIKDARGFFDQMPSKDVISW 180

Query: 266 TELIHMYVKKGGINEAFKLFRQMNMDGELKPDPLTISSILPACGRMAAHKHGKEIHGYVL 325
           TELIHMYVKKGGINEAFKLFRQMNMDGELKPDPLTISSILPACGRMAAHKHGKEIHGYVL
Sbjct: 181 TELIHMYVKKGGINEAFKLFRQMNMDGELKPDPLTISSILPACGRMAAHKHGKEIHGYVL 240

Query: 326 KNGFDENLIVQNALVDMYVKSGCIQSASKTFSMMKEKDMVSWSIMTLGYSLHGQGKLGVG 385
           KNGFDENLIVQNALVDMYVKSGCIQSASKTFSMMKEKDMVSWSIMTLGYSLHGQGKLGVG
Sbjct: 241 KNGFDENLIVQNALVDMYVKSGCIQSASKTFSMMKEKDMVSWSIMTLGYSLHGQGKLGVG 300

Query: 386 LFREMEKNLKMHRDEITYTAVLHACTTANMVDEGDFYFSRITKPTVAHIALKVALLARAG 445
           LFREMEKNLKMHRDEITYTAVLHACTTANMVDEGDFYFSRITKPTVAHIALKVALLARAG
Sbjct: 301 LFREMEKNLKMHRDEITYTAVLHACTTANMVDEGDFYFSRITKPTVAHIALKVALLARAG 360

Query: 446 RLDEARTFVEKKKLNKHPEILRALLDGCRNHRQQKLGKRIIEQLCDLEPLNTENYILLSN 505
           RLDEARTFVEKKKLNKHPEILRALLDGCRNHRQQKLGKRIIEQLCDLEPLNTENYILLSN
Sbjct: 361 RLDEARTFVEKKKLNKHPEILRALLDGCRNHRQQKLGKRIIEQLCDLEPLNTENYILLSN 420

Query: 506 WYACNKKWDMVEELRETIRDMGLRPKKAYSWIEFCNKIHVFGTGDVSHPRSQNIYWNLQC 565
           WYACNKKWDMVEELRETIRDMGLRPKKAYSWIEFCNKIHVF TGDVSHPRSQNIYWNLQC
Sbjct: 421 WYACNKKWDMVEELRETIRDMGLRPKKAYSWIEFCNKIHVFVTGDVSHPRSQNIYWNLQC 480

Query: 566 LMKKMEEDGSKTNPEFSLHDVDEERECVPIGHSELLAISFGLISTEAGRTIRITKNLRVC 625
           LMKKMEEDGSKTNPEFSLHDVDEERECVPIGHSELLAISFGLISTEAGRTIRITKNLRVC
Sbjct: 481 LMKKMEEDGSKTNPEFSLHDVDEERECVPIGHSELLAISFGLISTEAGRTIRITKNLRVC 540

Query: 626 HSCHESAKFISKMVGREIIVKDPYVFHHFKDGCCSCENFC 665
           HSCHESAKFISKMVGREIIVKDPYVFHHFKDGCCSCENFC
Sbjct: 541 HSCHESAKFISKMVGREIIVKDPYVFHHFKDGCCSCENFC 580

BLAST of MELO.jh008690.1 vs. ExPASy Swiss-Prot
Match: Q9SN39 (Pentatricopeptide repeat-containing protein DOT4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DOT4 PE=2 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 5.2e-122
Identity = 230/623 (36.92%), Postives = 360/623 (57.78%), Query Frame = 0

Query: 48  AHQVFDEIPIWDTFAWNNLIQTHLTNGDWGHVISIYRQMLFRGVRPDKHTLPRIICATRQ 107
           A +VFDE+   D  +WN++I  +++NG     +S++ QML  G+  D  T+  +      
Sbjct: 249 ARKVFDEMTERDVISWNSIINGYVSNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGCAD 308

Query: 108 YGDLPVGKQLHAQAFKLGFSSDLYVLTSLIELYGILDSADTAKWLHDKSTCRNSVSWTIL 167
              + +G+ +H+   K  FS +     +L+++Y      D+AK +  + + R+ VS+T +
Sbjct: 309 SRLISLGRAVHSIGVKACFSREDRFCNTLLDMYSKCGDLDSAKAVFREMSDRSVVSYTSM 368

Query: 168 AKLYLREDKPSFAIDLFYQMVELADDIDSVALATAIGACGALKMLHHGRNIHHLARIHGL 227
              Y RE     A+ LF +M E     D   +   +  C   ++L  G+ +H   + + L
Sbjct: 369 IAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTAVLNCCARYRLLDEGKRVHEWIKENDL 428

Query: 228 EFNILVSNSLLKMYLDCDSIKDARGFFDQMPSKDVISWTELIHMYVKKGGINEAFKLFRQ 287
            F+I VSN+L+ MY  C S+++A   F +M  KD+ISW  +I  Y K    NEA  LF  
Sbjct: 429 GFDIFVSNALMDMYAKCGSMQEAELVFSEMRVKDIISWNTIIGGYSKNCYANEALSLFNL 488

Query: 288 MNMDGELKPDPLTISSILPACGRMAAHKHGKEIHGYVLKNGFDENLIVQNALVDMYVKSG 347
           +  +    PD  T++ +LPAC  ++A   G+EIHGY+++NG+  +  V N+LVDMY K G
Sbjct: 489 LLEEKRFSPDERTVACVLPACASLSAFDKGREIHGYIMRNGYFSDRHVANSLVDMYAKCG 548

Query: 348 CIQSASKTFSMMKEKDMVSWSIMTLGYSLHGQGKLGVGLFREMEKNLKMHRDEITYTAVL 407
            +  A   F  +  KD+VSW++M  GY +HG GK  + LF +M +   +  DEI++ ++L
Sbjct: 549 ALLLAHMLFDDIASKDLVSWTVMIAGYGMHGFGKEAIALFNQM-RQAGIEADEISFVSLL 608

Query: 408 HACTTANMVDEGDFYFSRI-----TKPTVAHIALKVALLARAGRLDEARTFVEKKKLNKH 467
           +AC+ + +VDEG  +F+ +      +PTV H A  V +LAR G L +A  F+E   +   
Sbjct: 609 YACSHSGLVDEGWRFFNIMRHECKIEPTVEHYACIVDMLARTGDLIKAYRFIENMPIPPD 668

Query: 468 PEILRALLDGCRNHRQQKLGKRIIEQLCDLEPLNTENYILLSNWYACNKKWDMVEELRET 527
             I  ALL GCR H   KL +++ E++ +LEP NT  Y+L++N YA  +KW+ V+ LR+ 
Sbjct: 669 ATIWGALLCGCRIHHDVKLAEKVAEKVFELEPENTGYYVLMANIYAEAEKWEQVKRLRKR 728

Query: 528 IRDMGLRPKKAYSWIEFCNKIHVFGTGDVSHPRSQNIYWNLQCLMKKMEEDGSKTNPEFS 587
           I   GLR     SWIE   ++++F  GD S+P ++NI   L+ +  +M E+G     +++
Sbjct: 729 IGQRGLRKNPGCSWIEIKGRVNIFVAGDSSNPETENIEAFLRKVRARMIEEGYSPLTKYA 788

Query: 588 LHDVDE-ERECVPIGHSELLAISFGLISTEAGRTIRITKNLRVCHSCHESAKFISKMVGR 647
           L D +E E+E    GHSE LA++ G+IS+  G+ IR+TKNLRVC  CHE AKF+SK+  R
Sbjct: 789 LIDAEEMEKEEALCGHSEKLAMALGIISSGHGKIIRVTKNLRVCGDCHEMAKFMSKLTRR 848

Query: 648 EIIVKDPYVFHHFKDGCCSCENF 665
           EI+++D   FH FKDG CSC  F
Sbjct: 849 EIVLRDSNRFHQFKDGHCSCRGF 870

BLAST of MELO.jh008690.1 vs. ExPASy Swiss-Prot
Match: Q9LTV8 (Pentatricopeptide repeat-containing protein At3g12770 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H43 PE=2 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 4.1e-119
Identity = 222/631 (35.18%), Postives = 357/631 (56.58%), Query Frame = 0

Query: 41  NYADLCVAHQVFDEIPIWDTFAWNNLIQTHLTNGDWGHVISIYRQMLFRGVRPDKHTLPR 100
           ++ D+  A QVFD++P    F WN +I+ +  N  +   + +Y  M    V PD  T P 
Sbjct: 65  SFGDITFARQVFDDLPRPQIFPWNAIIRGYSRNNHFQDALLMYSNMQLARVSPDSFTFPH 124

Query: 101 IICATRQYGDLPVGKQLHAQAFKLGFSSDLYVLTSLIELYGILDSADTAKWLHDKSTC-- 160
           ++ A      L +G+ +HAQ F+LGF +D++V   LI LY       +A+ + +      
Sbjct: 125 LLKACSGLSHLQMGRFVHAQVFRLGFDADVFVQNGLIALYAKCRRLGSARTVFEGLPLPE 184

Query: 161 RNSVSWTILAKLYLREDKPSFAIDLFYQMVELADDIDSVALATAIGACGALKMLHHGRNI 220
           R  VSWT +   Y +  +P  A+++F QM ++    D VAL + + A   L+ L  GR+I
Sbjct: 185 RTIVSWTAIVSAYAQNGEPMEALEIFSQMRKMDVKPDWVALVSVLNAFTCLQDLKQGRSI 244

Query: 221 HHLARIHGLEFNILVSNSLLKMYLDCDSIKDARGFFDQMPSKDVISWTELIHMYVKKGGI 280
           H      GLE    +  SL  MY  C  +  A+  FD+M S ++I W  +I  Y K G  
Sbjct: 245 HASVVKMGLEIEPDLLISLNTMYAKCGQVATAKILFDKMKSPNLILWNAMISGYAKNGYA 304

Query: 281 NEAFKLFRQMNMDGELKPDPLTISSILPACGRMAAHKHGKEIHGYVLKNGFDENLIVQNA 340
            EA  +F +M ++ +++PD ++I+S + AC ++ + +  + ++ YV ++ + +++ + +A
Sbjct: 305 REAIDMFHEM-INKDVRPDTISITSAISACAQVGSLEQARSMYEYVGRSDYRDDVFISSA 364

Query: 341 LVDMYVKSGCIQSASKTFSMMKEKDMVSWSIMTLGYSLHGQGKLGVGLFREMEKNLKMHR 400
           L+DM+ K G ++ A   F    ++D+V WS M +GY LHG+ +  + L+R ME+   +H 
Sbjct: 365 LIDMFAKCGSVEGARLVFDRTLDRDVVVWSAMIVGYGLHGRAREAISLYRAMERG-GVHP 424

Query: 401 DEITYTAVLHACTTANMVDEGDFYFSRIT----KPTVAHIALKVALLARAGRLDEARTFV 460
           +++T+  +L AC  + MV EG ++F+R+      P   H A  + LL RAG LD+A   +
Sbjct: 425 NDVTFLGLLMACNHSGMVREGWWFFNRMADHKINPQQQHYACVIDLLGRAGHLDQAYEVI 484

Query: 461 EKKKLNKHPEILRALLDGCRNHRQQKLGKRIIEQLCDLEPLNTENYILLSNWYACNKKWD 520
           +   +     +  ALL  C+ HR  +LG+   +QL  ++P NT +Y+ LSN YA  + WD
Sbjct: 485 KCMPVQPGVTVWGALLSACKKHRHVELGEYAAQQLFSIDPSNTGHYVQLSNLYAAARLWD 544

Query: 521 MVEELRETIRDMGLRPKKAYSWIEFCNKIHVFGTGDVSHPRSQNIYWNLQCLMKKMEEDG 580
            V E+R  +++ GL      SW+E   ++  F  GD SHPR + I   ++ +  +++E G
Sbjct: 545 RVAEVRVRMKEKGLNKDVGCSWVEVRGRLEAFRVGDKSHPRYEEIERQVEWIESRLKEGG 604

Query: 581 SKTNPEFSLHDV-DEERECVPIGHSELLAISFGLISTEAGRTIRITKNLRVCHSCHESAK 640
              N + SLHD+ DEE E     HSE +AI++GLIST  G  +RITKNLR C +CH + K
Sbjct: 605 FVANKDASLHDLNDEEAEETLCSHSERIAIAYGLISTPQGTPLRITKNLRACVNCHAATK 664

Query: 641 FISKMVGREIIVKDPYVFHHFKDGCCSCENF 665
            ISK+V REI+V+D   FHHFKDG CSC ++
Sbjct: 665 LISKLVDREIVVRDTNRFHHFKDGVCSCGDY 693

BLAST of MELO.jh008690.1 vs. ExPASy Swiss-Prot
Match: Q9M9E2 (Pentatricopeptide repeat-containing protein At1g15510, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PCMP-H73 PE=1 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 7.3e-108
Identity = 229/623 (36.76%), Postives = 338/623 (54.25%), Query Frame = 0

Query: 44  DLCVAHQVFDEIPIWDTFAWNNLIQTHLTNGDWGHVISIYRQMLFRGVRPDKHTLPRIIC 103
           D+  A  +FD +P  D  +WN +I  +  NG     + ++  M    V PD  TL  +I 
Sbjct: 246 DVKSARLLFDRMPRRDIISWNAMISGYFENGMCHEGLELFFAMRGLSVDPDLMTLTSVIS 305

Query: 104 ATRQYGDLPVGKQLHAQAFKLGFSSDLYVLTSLIELYGILDSADTAKWLHDKSTCRNSVS 163
           A    GD  +G+ +HA     GF+ D+ V  SL ++Y    S   A+ L  +   ++ VS
Sbjct: 306 ACELLGDRRLGRDIHAYVITTGFAVDISVCNSLTQMYLNAGSWREAEKLFSRMERKDIVS 365

Query: 164 WTILAKLYLREDKPSFAIDLFYQMVELADDIDSVALATAIGACGALKMLHHGRNIHHLAR 223
           WT +   Y     P  AID +  M + +   D + +A  + AC  L  L  G  +H LA 
Sbjct: 366 WTTMISGYEYNFLPDKAIDTYRMMDQDSVKPDEITVAAVLSACATLGDLDTGVELHKLAI 425

Query: 224 IHGLEFNILVSNSLLKMYLDCDSIKDARGFFDQMPSKDVISWTELIHMYVKKGGINEAFK 283
              L   ++V+N+L+ MY  C  I  A   F  +P K+VISWT +I          EA  
Sbjct: 426 KARLISYVIVANNLINMYSKCKCIDKALDIFHNIPRKNVISWTSIIAGLRLNNRCFEALI 485

Query: 284 LFRQMNMDGELKPDPLTISSILPACGRMAAHKHGKEIHGYVLKNGFDENLIVQNALVDMY 343
             RQM M   L+P+ +T+++ L AC R+ A   GKEIH +VL+ G   +  + NAL+DMY
Sbjct: 486 FLRQMKM--TLQPNAITLTAALAACARIGALMCGKEIHAHVLRTGVGLDDFLPNALLDMY 545

Query: 344 VKSGCIQSASKTFSMMKEKDMVSWSIMTLGYSLHGQGKLGVGLFREMEKNLKMHRDEITY 403
           V+ G + +A   F+  K KD+ SW+I+  GYS  GQG + V LF  M K+ ++  DEIT+
Sbjct: 546 VRCGRMNTAWSQFNSQK-KDVTSWNILLTGYSERGQGSMVVELFDRMVKS-RVRPDEITF 605

Query: 404 TAVLHACTTANMVDEGDFYFSRI----TKPTVAHIALKVALLARAGRLDEARTFVEKKKL 463
            ++L  C+ + MV +G  YFS++      P + H A  V LL RAG L EA  F++K  +
Sbjct: 606 ISLLCGCSKSQMVRQGLMYFSKMEDYGVTPNLKHYACVVDLLGRAGELQEAHKFIQKMPV 665

Query: 464 NKHPEILRALLDGCRNHRQQKLGKRIIEQLCDLEPLNTENYILLSNWYACNKKWDMVEEL 523
              P +  ALL+ CR H +  LG+   + + +L+  +   YILL N YA   KW  V ++
Sbjct: 666 TPDPAVWGALLNACRIHHKIDLGELSAQHIFELDKKSVGYYILLCNLYADCGKWREVAKV 725

Query: 524 RETIRDMGLRPKKAYSWIEFCNKIHVFGTGDVSHPRSQNIYWNLQCLMKKMEEDG-SKTN 583
           R  +++ GL      SW+E   K+H F + D  HP+++ I   L+   +KM E G +K +
Sbjct: 726 RRMMKENGLTVDAGCSWVEVKGKVHAFLSDDKYHPQTKEINTVLEGFYEKMSEVGLTKIS 785

Query: 584 PEFSLHDVDEERECVPIGHSELLAISFGLISTEAGRTIRITKNLRVCHSCHESAKFISKM 643
              S+ + +  R+ +  GHSE  AI+FGLI+T  G  I +TKNL +C +CH++ KFISK 
Sbjct: 786 ESSSMDETEISRDEIFCGHSERKAIAFGLINTVPGMPIWVTKNLSMCENCHDTVKFISKT 845

Query: 644 VGREIIVKDPYVFHHFKDGCCSC 662
           V REI V+D   FHHFKDG CSC
Sbjct: 846 VRREISVRDAEHFHHFKDGECSC 864

BLAST of MELO.jh008690.1 vs. ExPASy Swiss-Prot
Match: Q9SHZ8 (Pentatricopeptide repeat-containing protein At2g22070 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H41 PE=3 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 1.0e-106
Identity = 234/731 (32.01%), Postives = 371/731 (50.75%), Query Frame = 0

Query: 14  ITQKPYHAYHRHPPFNNLPHVRT-----TTVENYA---DLCVAHQVFDEIPIWDTFAWNN 73
           +  K  +A H    F+ +P +RT     T +  Y+   D+    + FD++P  D+ +W  
Sbjct: 58  VYSKTGYALHARKLFDEMP-LRTAFSWNTVLSAYSKRGDMDSTCEFFDQLPQRDSVSWTT 117

Query: 74  LIQTHLTNGDWGHVISIYRQMLFRGVRPDKHTLPRIICATRQYGDLPVGKQLHAQAFKLG 133
           +I  +   G +   I +   M+  G+ P + TL  ++ +      +  GK++H+   KLG
Sbjct: 118 MIVGYKNIGQYHKAIRVMGDMVKEGIEPTQFTLTNVLASVAATRCMETGKKVHSFIVKLG 177

Query: 134 FSSDLYVLTSLIELYGILDSADTAKWLHDKSTCRNSVSWTILAKLYLREDKPSFAIDLFY 193
              ++ V  SL+ +Y        AK++ D+   R+  SW  +  L+++  +   A+  F 
Sbjct: 178 LRGNVSVSNSLLNMYAKCGDPMMAKFVFDRMVVRDISSWNAMIALHMQVGQMDLAMAQFE 237

Query: 194 QMVE-----------------------------LADDI---DSVALATAIGACGALKMLH 253
           QM E                             L D +   D   LA+ + AC  L+ L 
Sbjct: 238 QMAERDIVTWNSMISGFNQRGYDLRALDIFSKMLRDSLLSPDRFTLASVLSACANLEKLC 297

Query: 254 HGRNIHHLARIHGLEFNILVSNSLLKMYLDCDSIKDARGFFDQ----------------- 313
            G+ IH      G + + +V N+L+ MY  C  ++ AR   +Q                 
Sbjct: 298 IGKQIHSHIVTTGFDISGIVLNALISMYSRCGGVETARRLIEQRGTKDLKIEGFTALLDG 357

Query: 314 ----------------MPSKDVISWTELIHMYVKKGGINEAFKLFRQMNMDGELKPDPLT 373
                           +  +DV++WT +I  Y + G   EA  LFR M + G  +P+  T
Sbjct: 358 YIKLGDMNQAKNIFVSLKDRDVVAWTAMIVGYEQHGSYGEAINLFRSM-VGGGQRPNSYT 417

Query: 374 ISSILPACGRMAAHKHGKEIHGYVLKNGFDENLIVQNALVDMYVKSGCIQSASKTFSMMK 433
           ++++L     +A+  HGK+IHG  +K+G   ++ V NAL+ MY K+G I SAS+ F +++
Sbjct: 418 LAAMLSVASSLASLSHGKQIHGSAVKSGEIYSVSVSNALITMYAKAGNITSASRAFDLIR 477

Query: 434 -EKDMVSWSIMTLGYSLHGQGKLGVGLFREMEKNLKMHRDEITYTAVLHACTTANMVDEG 493
            E+D VSW+ M +  + HG  +  + LF  M     +  D ITY  V  ACT A +V++G
Sbjct: 478 CERDTVSWTSMIIALAQHGHAEEALELFETMLME-GLRPDHITYVGVFSACTHAGLVNQG 537

Query: 494 DFYFSRITK-----PTVAHIALKVALLARAGRLDEARTFVEKKKLNKHPEILRALLDGCR 553
             YF  +       PT++H A  V L  RAG L EA+ F+EK  +        +LL  CR
Sbjct: 538 RQYFDMMKDVDKIIPTLSHYACMVDLFGRAGLLQEAQEFIEKMPIEPDVVTWGSLLSACR 597

Query: 554 NHRQQKLGKRIIEQLCDLEPLNTENYILLSNWYACNKKWDMVEELRETIRDMGLRPKKAY 613
            H+   LGK   E+L  LEP N+  Y  L+N Y+   KW+   ++R++++D  ++ ++ +
Sbjct: 598 VHKNIDLGKVAAERLLLLEPENSGAYSALANLYSACGKWEEAAKIRKSMKDGRVKKEQGF 657

Query: 614 SWIEFCNKIHVFGTGDVSHPRSQNIYWNLQCLMKKMEEDGSKTNPEFSLHDVDEE-RECV 665
           SWIE  +K+HVFG  D +HP    IY  ++ +  ++++ G   +    LHD++EE +E +
Sbjct: 658 SWIEVKHKVHVFGVEDGTHPEKNEIYMTMKKIWDEIKKMGYVPDTASVLHDLEEEVKEQI 717

BLAST of MELO.jh008690.1 vs. ExPASy Swiss-Prot
Match: Q3E6Q1 (Pentatricopeptide repeat-containing protein At1g11290, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PCMP-H40 PE=2 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 6.8e-106
Identity = 210/622 (33.76%), Postives = 339/622 (54.50%), Query Frame = 0

Query: 48  AHQVFDEIPIWDTFAWNNLIQTHLTNGDWGHVISIYRQMLFRGVRPDKHTLPRIICATRQ 107
           A +VFD +P  D  +WN ++  +  NG     + + + M    ++P   T+  ++ A   
Sbjct: 189 ARKVFDRMPERDLVSWNTIVAGYSQNGMARMALEMVKSMCEENLKPSFITIVSVLPAVSA 248

Query: 108 YGDLPVGKQLHAQAFKLGFSSDLYVLTSLIELYGILDSADTAKWLHDKSTCRNSVSWTIL 167
              + VGK++H  A + GF S + + T+L+++Y    S +TA+ L D    RN VSW  +
Sbjct: 249 LRLISVGKEIHGYAMRSGFDSLVNISTALVDMYAKCGSLETARQLFDGMLERNVVSWNSM 308

Query: 168 AKLYLREDKPSFAIDLFYQMVELADDIDSVALATAIGACGALKMLHHGRNIHHLARIHGL 227
              Y++ + P  A+ +F +M++       V++  A+ AC  L  L  GR IH L+   GL
Sbjct: 309 IDAYVQNENPKEAMLIFQKMLDEGVKPTDVSVMGALHACADLGDLERGRFIHKLSVELGL 368

Query: 228 EFNILVSNSLLKMYLDCDSIKDARGFFDQMPSKDVISWTELIHMYVKKGGINEAFKLFRQ 287
           + N+ V NSL+ MY  C  +  A   F ++ S+ ++SW  +I  + + G   +A   F Q
Sbjct: 369 DRNVSVVNSLISMYCKCKEVDTAASMFGKLQSRTLVSWNAMILGFAQNGRPIDALNYFSQ 428

Query: 288 MNMDGELKPDPLTISSILPACGRMAAHKHGKEIHGYVLKNGFDENLIVQNALVDMYVKSG 347
           M     +KPD  T  S++ A   ++   H K IHG V+++  D+N+ V  ALVDMY K G
Sbjct: 429 MR-SRTVKPDTFTYVSVITAIAELSITHHAKWIHGVVMRSCLDKNVFVTTALVDMYAKCG 488

Query: 348 CIQSASKTFSMMKEKDMVSWSIMTLGYSLHGQGKLGVGLFREMEKNLKMHRDEITYTAVL 407
            I  A   F MM E+ + +W+ M  GY  HG GK  + LF EM+K   +  + +T+ +V+
Sbjct: 489 AIMIARLIFDMMSERHVTTWNAMIDGYGTHGFGKAALELFEEMQKG-TIKPNGVTFLSVI 548

Query: 408 HACTTANMVDEG--DFYFSR---ITKPTVAHIALKVALLARAGRLDEARTFVEKKKLNKH 467
            AC+ + +V+ G   FY  +     + ++ H    V LL RAGRL+EA  F+ +  +   
Sbjct: 549 SACSHSGLVEAGLKCFYMMKENYSIELSMDHYGAMVDLLGRAGRLNEAWDFIMQMPVKPA 608

Query: 468 PEILRALLDGCRNHRQQKLGKRIIEQLCDLEPLNTENYILLSNWYACNKKWDMVEELRET 527
             +  A+L  C+ H+     ++  E+L +L P +   ++LL+N Y     W+ V ++R +
Sbjct: 609 VNVYGAMLGACQIHKNVNFAEKAAERLFELNPDDGGYHVLLANIYRAASMWEKVGQVRVS 668

Query: 528 IRDMGLRPKKAYSWIEFCNKIHVFGTGDVSHPRSQNIYWNLQCLMKKMEEDGSKTNPEFS 587
           +   GLR     S +E  N++H F +G  +HP S+ IY  L+ L+  ++E G   +    
Sbjct: 669 MLRQGLRKTPGCSMVEIKNEVHSFFSGSTAHPDSKKIYAFLEKLICHIKEAGYVPDTNLV 728

Query: 588 LHDVDEERECVPIGHSELLAISFGLISTEAGRTIRITKNLRVCHSCHESAKFISKMVGRE 647
           L   ++ +E +   HSE LAISFGL++T AG TI + KNLRVC  CH + K+IS + GRE
Sbjct: 729 LGVENDVKEQLLSTHSEKLAISFGLLNTTAGTTIHVRKNLRVCADCHNATKYISLVTGRE 788

Query: 648 IIVKDPYVFHHFKDGCCSCENF 665
           I+V+D   FHHFK+G CSC ++
Sbjct: 789 IVVRDMQRFHHFKNGACSCGDY 808

BLAST of MELO.jh008690.1 vs. ExPASy TrEMBL
Match: A0A1S3CPR5 (pentatricopeptide repeat-containing protein DOT4, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103502829 PE=3 SV=1)

HSP 1 Score: 1384 bits (3581), Expect = 0.0
Identity = 665/665 (100.00%), Postives = 665/665 (100.00%), Query Frame = 0

Query: 1   MNLLLSTHTHCLPITQKPYHAYHRHPPFNNLPHVRTTTVENYADLCVAHQVFDEIPIWDT 60
           MNLLLSTHTHCLPITQKPYHAYHRHPPFNNLPHVRTTTVENYADLCVAHQVFDEIPIWDT
Sbjct: 1   MNLLLSTHTHCLPITQKPYHAYHRHPPFNNLPHVRTTTVENYADLCVAHQVFDEIPIWDT 60

Query: 61  FAWNNLIQTHLTNGDWGHVISIYRQMLFRGVRPDKHTLPRIICATRQYGDLPVGKQLHAQ 120
           FAWNNLIQTHLTNGDWGHVISIYRQMLFRGVRPDKHTLPRIICATRQYGDLPVGKQLHAQ
Sbjct: 61  FAWNNLIQTHLTNGDWGHVISIYRQMLFRGVRPDKHTLPRIICATRQYGDLPVGKQLHAQ 120

Query: 121 AFKLGFSSDLYVLTSLIELYGILDSADTAKWLHDKSTCRNSVSWTILAKLYLREDKPSFA 180
           AFKLGFSSDLYVLTSLIELYGILDSADTAKWLHDKSTCRNSVSWTILAKLYLREDKPSFA
Sbjct: 121 AFKLGFSSDLYVLTSLIELYGILDSADTAKWLHDKSTCRNSVSWTILAKLYLREDKPSFA 180

Query: 181 IDLFYQMVELADDIDSVALATAIGACGALKMLHHGRNIHHLARIHGLEFNILVSNSLLKM 240
           IDLFYQMVELADDIDSVALATAIGACGALKMLHHGRNIHHLARIHGLEFNILVSNSLLKM
Sbjct: 181 IDLFYQMVELADDIDSVALATAIGACGALKMLHHGRNIHHLARIHGLEFNILVSNSLLKM 240

Query: 241 YLDCDSIKDARGFFDQMPSKDVISWTELIHMYVKKGGINEAFKLFRQMNMDGELKPDPLT 300
           YLDCDSIKDARGFFDQMPSKDVISWTELIHMYVKKGGINEAFKLFRQMNMDGELKPDPLT
Sbjct: 241 YLDCDSIKDARGFFDQMPSKDVISWTELIHMYVKKGGINEAFKLFRQMNMDGELKPDPLT 300

Query: 301 ISSILPACGRMAAHKHGKEIHGYVLKNGFDENLIVQNALVDMYVKSGCIQSASKTFSMMK 360
           ISSILPACGRMAAHKHGKEIHGYVLKNGFDENLIVQNALVDMYVKSGCIQSASKTFSMMK
Sbjct: 301 ISSILPACGRMAAHKHGKEIHGYVLKNGFDENLIVQNALVDMYVKSGCIQSASKTFSMMK 360

Query: 361 EKDMVSWSIMTLGYSLHGQGKLGVGLFREMEKNLKMHRDEITYTAVLHACTTANMVDEGD 420
           EKDMVSWSIMTLGYSLHGQGKLGVGLFREMEKNLKMHRDEITYTAVLHACTTANMVDEGD
Sbjct: 361 EKDMVSWSIMTLGYSLHGQGKLGVGLFREMEKNLKMHRDEITYTAVLHACTTANMVDEGD 420

Query: 421 FYFSRITKPTVAHIALKVALLARAGRLDEARTFVEKKKLNKHPEILRALLDGCRNHRQQK 480
           FYFSRITKPTVAHIALKVALLARAGRLDEARTFVEKKKLNKHPEILRALLDGCRNHRQQK
Sbjct: 421 FYFSRITKPTVAHIALKVALLARAGRLDEARTFVEKKKLNKHPEILRALLDGCRNHRQQK 480

Query: 481 LGKRIIEQLCDLEPLNTENYILLSNWYACNKKWDMVEELRETIRDMGLRPKKAYSWIEFC 540
           LGKRIIEQLCDLEPLNTENYILLSNWYACNKKWDMVEELRETIRDMGLRPKKAYSWIEFC
Sbjct: 481 LGKRIIEQLCDLEPLNTENYILLSNWYACNKKWDMVEELRETIRDMGLRPKKAYSWIEFC 540

Query: 541 NKIHVFGTGDVSHPRSQNIYWNLQCLMKKMEEDGSKTNPEFSLHDVDEERECVPIGHSEL 600
           NKIHVFGTGDVSHPRSQNIYWNLQCLMKKMEEDGSKTNPEFSLHDVDEERECVPIGHSEL
Sbjct: 541 NKIHVFGTGDVSHPRSQNIYWNLQCLMKKMEEDGSKTNPEFSLHDVDEERECVPIGHSEL 600

Query: 601 LAISFGLISTEAGRTIRITKNLRVCHSCHESAKFISKMVGREIIVKDPYVFHHFKDGCCS 660
           LAISFGLISTEAGRTIRITKNLRVCHSCHESAKFISKMVGREIIVKDPYVFHHFKDGCCS
Sbjct: 601 LAISFGLISTEAGRTIRITKNLRVCHSCHESAKFISKMVGREIIVKDPYVFHHFKDGCCS 660

Query: 661 CENFC 665
           CENFC
Sbjct: 661 CENFC 665

BLAST of MELO.jh008690.1 vs. ExPASy TrEMBL
Match: A0A0A0L9N4 (DYW_deaminase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G722890 PE=3 SV=1)

HSP 1 Score: 1230 bits (3183), Expect = 0.0
Identity = 593/624 (95.03%), Postives = 609/624 (97.60%), Query Frame = 0

Query: 1   MNLLLSTHTHCLPITQKPYHAYHRHPPFNNLPHVRTTTVENYADLCVAHQVFDEIPIWDT 60
           MNLLLSTHTHCLPITQKP HAYHRHPPFNNLPHVRT TVENYA+LCVAHQVFD+IPIWDT
Sbjct: 1   MNLLLSTHTHCLPITQKPNHAYHRHPPFNNLPHVRTMTVENYANLCVAHQVFDDIPIWDT 60

Query: 61  FAWNNLIQTHLTNGDWGHVISIYRQMLFRGVRPDKHTLPRIICATRQYGDLPVGKQLHAQ 120
           FAWNNLIQTHLTNGD GHVIS YRQMLFRGVRPDKHTLPRIICATRQYGDL VGKQLHAQ
Sbjct: 61  FAWNNLIQTHLTNGDLGHVISTYRQMLFRGVRPDKHTLPRIICATRQYGDLQVGKQLHAQ 120

Query: 121 AFKLGFSSDLYVLTSLIELYGILDSADTAKWLHDKSTCRNSVSWTILAKLYLREDKPSFA 180
           AFKLGFSS+LYVLTSLIELYGILDSADTAKWLHDKSTCRNSVSWT+LAKLYLREDKPS A
Sbjct: 121 AFKLGFSSNLYVLTSLIELYGILDSADTAKWLHDKSTCRNSVSWTVLAKLYLREDKPSLA 180

Query: 181 IDLFYQMVELADDIDSVALATAIGACGALKMLHHGRNIHHLARIHGLEFNILVSNSLLKM 240
           +DLFYQMVELADDID+VALATAIGACGALKMLHHGRNIHHLAR+HGLEFNILVSNSLLKM
Sbjct: 181 LDLFYQMVELADDIDAVALATAIGACGALKMLHHGRNIHHLARVHGLEFNILVSNSLLKM 240

Query: 241 YLDCDSIKDARGFFDQMPSKDVISWTELIHMYVKKGGINEAFKLFRQMNMDGELKPDPLT 300
           Y+DCDSIKDARGFFDQMPSKD+ISWTELIHMYVKKGGINEAFKLFRQMNMDGELKPDP T
Sbjct: 241 YIDCDSIKDARGFFDQMPSKDIISWTELIHMYVKKGGINEAFKLFRQMNMDGELKPDPRT 300

Query: 301 ISSILPACGRMAAHKHGKEIHGYVLKNGFDENLIVQNALVDMYVKSGCIQSASKTFSMMK 360
           ISSILPACGRMAAHKHGKEIHGYV+KN FDENLIVQNALVDMYVKSGCIQSASKTFSMMK
Sbjct: 301 ISSILPACGRMAAHKHGKEIHGYVVKNAFDENLIVQNALVDMYVKSGCIQSASKTFSMMK 360

Query: 361 EKDMVSWSIMTLGYSLHGQGKLGVGLFREMEKNLKMHRDEITYTAVLHACTTANMVDEGD 420
           EKDMVSWSIMTLGYSLHGQGKLGV LFREMEKN KM RDEITYTAVLHACTTANMVDEGD
Sbjct: 361 EKDMVSWSIMTLGYSLHGQGKLGVSLFREMEKNFKMRRDEITYTAVLHACTTANMVDEGD 420

Query: 421 FYFSRITKPTVAHIALKVALLARAGRLDEARTFVEKKKLNKHPEILRALLDGCRNHRQQK 480
            YFS ITKPTVAHIALKVALLARAGRLDEARTFVEKKKL+KHPEILRALLDGCRNHRQQK
Sbjct: 421 SYFSCITKPTVAHIALKVALLARAGRLDEARTFVEKKKLDKHPEILRALLDGCRNHRQQK 480

Query: 481 LGKRIIEQLCDLEPLNTENYILLSNWYACNKKWDMVEELRETIRDMGLRPKKAYSWIEFC 540
           LGKRIIEQLCDLEPLN ENYILLSNWYACN+KWDMVE+LRETIRDMGLRPKKAYSWIEFC
Sbjct: 481 LGKRIIEQLCDLEPLNAENYILLSNWYACNEKWDMVEKLRETIRDMGLRPKKAYSWIEFC 540

Query: 541 NKIHVFGTGDVSHPRSQNIYWNLQCLMKKMEEDGSKTNPEFSLHDVDEERECVPIGHSEL 600
           NKIHVFGTGDVSHPRSQNIYWNLQCLMK+MEEDGSK NP+FSLHDVDEERECVPIGHSEL
Sbjct: 541 NKIHVFGTGDVSHPRSQNIYWNLQCLMKEMEEDGSKPNPDFSLHDVDEERECVPIGHSEL 600

Query: 601 LAISFGLISTEAGRTIRITKNLRV 624
           LAISFGLISTEAGRTIRITKNLR+
Sbjct: 601 LAISFGLISTEAGRTIRITKNLRM 624

BLAST of MELO.jh008690.1 vs. ExPASy TrEMBL
Match: A0A5A7T6A7 (Pentatricopeptide repeat-containing protein DOT4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1593G00200 PE=3 SV=1)

HSP 1 Score: 1196 bits (3095), Expect = 0.0
Identity = 580/580 (100.00%), Postives = 580/580 (100.00%), Query Frame = 0

Query: 86  MLFRGVRPDKHTLPRIICATRQYGDLPVGKQLHAQAFKLGFSSDLYVLTSLIELYGILDS 145
           MLFRGVRPDKHTLPRIICATRQYGDLPVGKQLHAQAFKLGFSSDLYVLTSLIELYGILDS
Sbjct: 1   MLFRGVRPDKHTLPRIICATRQYGDLPVGKQLHAQAFKLGFSSDLYVLTSLIELYGILDS 60

Query: 146 ADTAKWLHDKSTCRNSVSWTILAKLYLREDKPSFAIDLFYQMVELADDIDSVALATAIGA 205
           ADTAKWLHDKSTCRNSVSWTILAKLYLREDKPSFAIDLFYQMVELADDIDSVALATAIGA
Sbjct: 61  ADTAKWLHDKSTCRNSVSWTILAKLYLREDKPSFAIDLFYQMVELADDIDSVALATAIGA 120

Query: 206 CGALKMLHHGRNIHHLARIHGLEFNILVSNSLLKMYLDCDSIKDARGFFDQMPSKDVISW 265
           CGALKMLHHGRNIHHLARIHGLEFNILVSNSLLKMYLDCDSIKDARGFFDQMPSKDVISW
Sbjct: 121 CGALKMLHHGRNIHHLARIHGLEFNILVSNSLLKMYLDCDSIKDARGFFDQMPSKDVISW 180

Query: 266 TELIHMYVKKGGINEAFKLFRQMNMDGELKPDPLTISSILPACGRMAAHKHGKEIHGYVL 325
           TELIHMYVKKGGINEAFKLFRQMNMDGELKPDPLTISSILPACGRMAAHKHGKEIHGYVL
Sbjct: 181 TELIHMYVKKGGINEAFKLFRQMNMDGELKPDPLTISSILPACGRMAAHKHGKEIHGYVL 240

Query: 326 KNGFDENLIVQNALVDMYVKSGCIQSASKTFSMMKEKDMVSWSIMTLGYSLHGQGKLGVG 385
           KNGFDENLIVQNALVDMYVKSGCIQSASKTFSMMKEKDMVSWSIMTLGYSLHGQGKLGVG
Sbjct: 241 KNGFDENLIVQNALVDMYVKSGCIQSASKTFSMMKEKDMVSWSIMTLGYSLHGQGKLGVG 300

Query: 386 LFREMEKNLKMHRDEITYTAVLHACTTANMVDEGDFYFSRITKPTVAHIALKVALLARAG 445
           LFREMEKNLKMHRDEITYTAVLHACTTANMVDEGDFYFSRITKPTVAHIALKVALLARAG
Sbjct: 301 LFREMEKNLKMHRDEITYTAVLHACTTANMVDEGDFYFSRITKPTVAHIALKVALLARAG 360

Query: 446 RLDEARTFVEKKKLNKHPEILRALLDGCRNHRQQKLGKRIIEQLCDLEPLNTENYILLSN 505
           RLDEARTFVEKKKLNKHPEILRALLDGCRNHRQQKLGKRIIEQLCDLEPLNTENYILLSN
Sbjct: 361 RLDEARTFVEKKKLNKHPEILRALLDGCRNHRQQKLGKRIIEQLCDLEPLNTENYILLSN 420

Query: 506 WYACNKKWDMVEELRETIRDMGLRPKKAYSWIEFCNKIHVFGTGDVSHPRSQNIYWNLQC 565
           WYACNKKWDMVEELRETIRDMGLRPKKAYSWIEFCNKIHVFGTGDVSHPRSQNIYWNLQC
Sbjct: 421 WYACNKKWDMVEELRETIRDMGLRPKKAYSWIEFCNKIHVFGTGDVSHPRSQNIYWNLQC 480

Query: 566 LMKKMEEDGSKTNPEFSLHDVDEERECVPIGHSELLAISFGLISTEAGRTIRITKNLRVC 625
           LMKKMEEDGSKTNPEFSLHDVDEERECVPIGHSELLAISFGLISTEAGRTIRITKNLRVC
Sbjct: 481 LMKKMEEDGSKTNPEFSLHDVDEERECVPIGHSELLAISFGLISTEAGRTIRITKNLRVC 540

Query: 626 HSCHESAKFISKMVGREIIVKDPYVFHHFKDGCCSCENFC 665
           HSCHESAKFISKMVGREIIVKDPYVFHHFKDGCCSCENFC
Sbjct: 541 HSCHESAKFISKMVGREIIVKDPYVFHHFKDGCCSCENFC 580

BLAST of MELO.jh008690.1 vs. ExPASy TrEMBL
Match: A0A5D3CFC2 (Pentatricopeptide repeat-containing protein DOT4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold25G00420 PE=3 SV=1)

HSP 1 Score: 1193 bits (3086), Expect = 0.0
Identity = 579/580 (99.83%), Postives = 579/580 (99.83%), Query Frame = 0

Query: 86  MLFRGVRPDKHTLPRIICATRQYGDLPVGKQLHAQAFKLGFSSDLYVLTSLIELYGILDS 145
           MLFRGVRPDKHTLPRIICATRQYGDLPVGKQLHAQAFKLGFSSDLYVLTSLIELYGILDS
Sbjct: 1   MLFRGVRPDKHTLPRIICATRQYGDLPVGKQLHAQAFKLGFSSDLYVLTSLIELYGILDS 60

Query: 146 ADTAKWLHDKSTCRNSVSWTILAKLYLREDKPSFAIDLFYQMVELADDIDSVALATAIGA 205
           ADTAKWLHDKSTCRNSVSWTILAKLYLREDKPSFAIDLFYQMVELADDIDSVALATAIGA
Sbjct: 61  ADTAKWLHDKSTCRNSVSWTILAKLYLREDKPSFAIDLFYQMVELADDIDSVALATAIGA 120

Query: 206 CGALKMLHHGRNIHHLARIHGLEFNILVSNSLLKMYLDCDSIKDARGFFDQMPSKDVISW 265
           CGALKMLHHGRNIHHLARIHGLEFNILVSNSLLKMYLDCDSIKDARGFFDQMPSKDVISW
Sbjct: 121 CGALKMLHHGRNIHHLARIHGLEFNILVSNSLLKMYLDCDSIKDARGFFDQMPSKDVISW 180

Query: 266 TELIHMYVKKGGINEAFKLFRQMNMDGELKPDPLTISSILPACGRMAAHKHGKEIHGYVL 325
           TELIHMYVKKGGINEAFKLFRQMNMDGELKPDPLTISSILPACGRMAAHKHGKEIHGYVL
Sbjct: 181 TELIHMYVKKGGINEAFKLFRQMNMDGELKPDPLTISSILPACGRMAAHKHGKEIHGYVL 240

Query: 326 KNGFDENLIVQNALVDMYVKSGCIQSASKTFSMMKEKDMVSWSIMTLGYSLHGQGKLGVG 385
           KNGFDENLIVQNALVDMYVKSGCIQSASKTFSMMKEKDMVSWSIMTLGYSLHGQGKLGVG
Sbjct: 241 KNGFDENLIVQNALVDMYVKSGCIQSASKTFSMMKEKDMVSWSIMTLGYSLHGQGKLGVG 300

Query: 386 LFREMEKNLKMHRDEITYTAVLHACTTANMVDEGDFYFSRITKPTVAHIALKVALLARAG 445
           LFREMEKNLKMHRDEITYTAVLHACTTANMVDEGDFYFSRITKPTVAHIALKVALLARAG
Sbjct: 301 LFREMEKNLKMHRDEITYTAVLHACTTANMVDEGDFYFSRITKPTVAHIALKVALLARAG 360

Query: 446 RLDEARTFVEKKKLNKHPEILRALLDGCRNHRQQKLGKRIIEQLCDLEPLNTENYILLSN 505
           RLDEARTFVEKKKLNKHPEILRALLDGCRNHRQQKLGKRIIEQLCDLEPLNTENYILLSN
Sbjct: 361 RLDEARTFVEKKKLNKHPEILRALLDGCRNHRQQKLGKRIIEQLCDLEPLNTENYILLSN 420

Query: 506 WYACNKKWDMVEELRETIRDMGLRPKKAYSWIEFCNKIHVFGTGDVSHPRSQNIYWNLQC 565
           WYACNKKWDMVEELRETIRDMGLRPKKAYSWIEFCNKIHVF TGDVSHPRSQNIYWNLQC
Sbjct: 421 WYACNKKWDMVEELRETIRDMGLRPKKAYSWIEFCNKIHVFVTGDVSHPRSQNIYWNLQC 480

Query: 566 LMKKMEEDGSKTNPEFSLHDVDEERECVPIGHSELLAISFGLISTEAGRTIRITKNLRVC 625
           LMKKMEEDGSKTNPEFSLHDVDEERECVPIGHSELLAISFGLISTEAGRTIRITKNLRVC
Sbjct: 481 LMKKMEEDGSKTNPEFSLHDVDEERECVPIGHSELLAISFGLISTEAGRTIRITKNLRVC 540

Query: 626 HSCHESAKFISKMVGREIIVKDPYVFHHFKDGCCSCENFC 665
           HSCHESAKFISKMVGREIIVKDPYVFHHFKDGCCSCENFC
Sbjct: 541 HSCHESAKFISKMVGREIIVKDPYVFHHFKDGCCSCENFC 580

BLAST of MELO.jh008690.1 vs. ExPASy TrEMBL
Match: A0A6J1I9E1 (pentatricopeptide repeat-containing protein DOT4, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111470851 PE=3 SV=1)

HSP 1 Score: 1149 bits (2973), Expect = 0.0
Identity = 546/665 (82.11%), Postives = 600/665 (90.23%), Query Frame = 0

Query: 1   MNLLLSTHTHCLPITQKPYHAYHRHPPFNNLPHVRTTTVENYADLCVAHQVFDEIPIWDT 60
           M+LLLST  H LP+TQKP H YHRH  FNN PHVRTTT E  A LCVAHQ+FD+IPIWDT
Sbjct: 1   MDLLLSTPIHRLPLTQKPNHTYHRHRLFNNPPHVRTTTAEKNAHLCVAHQLFDDIPIWDT 60

Query: 61  FAWNNLIQTHLTNGDWGHVISIYRQMLFRGVRPDKHTLPRIICATRQYGDLPVGKQLHAQ 120
           FAWNNLIQTHLT+GD GHVIS Y+QML RGVRPD HTLPR+ICA+R YGDL +GKQLHAQ
Sbjct: 61  FAWNNLIQTHLTSGDVGHVISTYQQMLSRGVRPDNHTLPRVICASRHYGDLQLGKQLHAQ 120

Query: 121 AFKLGFSSDLYVLTSLIELYGILDSADTAKWLHDKSTCRNSVSWTILAKLYLREDKPSFA 180
           AFKLG  S+LYV TSLIELYGILDSADTA+WLHDKS CRN+VSWT+LAKLYL EDKPSF+
Sbjct: 121 AFKLGLFSNLYVFTSLIELYGILDSADTARWLHDKSACRNAVSWTMLAKLYLMEDKPSFS 180

Query: 181 IDLFYQMVELADDIDSVALATAIGACGALKMLHHGRNIHHLARIHGLEFNILVSNSLLKM 240
           +DLFYQMVELA DID+VALATAIGACGA K+L HGRNIHH+ARIHGLEF++LVSN LLKM
Sbjct: 181 LDLFYQMVELAADIDAVALATAIGACGARKLLQHGRNIHHVARIHGLEFDVLVSNCLLKM 240

Query: 241 YLDCDSIKDARGFFDQMPSKDVISWTELIHMYVKKGGINEAFKLFRQMNMDGELKPDPLT 300
           YLDC SIKDARG F++MP +D+ISWT+LIH YVK GGINEA KLFRQMNMDGELKPDPLT
Sbjct: 241 YLDCSSIKDARGLFNRMPFRDIISWTDLIHFYVKNGGINEALKLFRQMNMDGELKPDPLT 300

Query: 301 ISSILPACGRMAAHKHGKEIHGYVLKNGFDENLIVQNALVDMYVKSGCIQSASKTFSMMK 360
           ISSILPACGR+AAHKHG+EIHGYVLKN FD+NLIVQNALVDMYVKSGCIQSA K FS MK
Sbjct: 301 ISSILPACGRIAAHKHGREIHGYVLKNDFDDNLIVQNALVDMYVKSGCIQSALKIFSRMK 360

Query: 361 EKDMVSWSIMTLGYSLHGQGKLGVGLFREMEKNLKMHRDEITYTAVLHACTTANMVDEGD 420
           EKDMVSW++M  GYSLHGQGKLGVGLFREM++N ++HRDEITYTAVL +C+TA+MV+EGD
Sbjct: 361 EKDMVSWTVMISGYSLHGQGKLGVGLFREMDRNFRVHRDEITYTAVLQSCSTASMVEEGD 420

Query: 421 FYFSRITKPTVAHIALKVALLARAGRLDEARTFVEKKKLNKHPEILRALLDGCRNHRQQK 480
           FYF+ IT+PT+AH  LKVALL RAGR DEARTFV+K KL+K+ EILRALLDGCR H Q K
Sbjct: 421 FYFNCITEPTMAHFVLKVALLGRAGRFDEARTFVDKHKLDKNSEILRALLDGCRKHHQHK 480

Query: 481 LGKRIIEQLCDLEPLNTENYILLSNWYACNKKWDMVEELRETIRDMGLRPKKAYSWIEFC 540
           LGKRIIEQLCDLEPLN ENY+LLSNWYA N++W+MVE+LR+TIRDMGLRPKKAYSW+EF 
Sbjct: 481 LGKRIIEQLCDLEPLNAENYVLLSNWYASNEEWEMVEKLRKTIRDMGLRPKKAYSWMEFR 540

Query: 541 NKIHVFGTGDVSHPRSQNIYWNLQCLMKKMEEDGSKTNPEFSLHDVDEERECVPIGHSEL 600
           NKIH FGTGDVSHPRSQ IYWNLQCLMKKMEEDG K N +F  HDVDEEREC PIGHSEL
Sbjct: 541 NKIHAFGTGDVSHPRSQAIYWNLQCLMKKMEEDGFKRNTDFRFHDVDEERECAPIGHSEL 600

Query: 601 LAISFGLISTEAGRTIRITKNLRVCHSCHESAKFISKMVGREIIVKDPYVFHHFKDGCCS 660
           LAISFGLISTEAGRTIRI+KNLRVCHSCHESAKFIS  VGREIIVKDPYVFHHFKDG CS
Sbjct: 601 LAISFGLISTEAGRTIRISKNLRVCHSCHESAKFISNKVGREIIVKDPYVFHHFKDGRCS 660

Query: 661 CENFC 665
           CE+FC
Sbjct: 661 CEDFC 665

BLAST of MELO.jh008690.1 vs. TAIR 10
Match: AT4G18750.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 439.9 bits (1130), Expect = 3.7e-123
Identity = 230/623 (36.92%), Postives = 360/623 (57.78%), Query Frame = 0

Query: 48  AHQVFDEIPIWDTFAWNNLIQTHLTNGDWGHVISIYRQMLFRGVRPDKHTLPRIICATRQ 107
           A +VFDE+   D  +WN++I  +++NG     +S++ QML  G+  D  T+  +      
Sbjct: 249 ARKVFDEMTERDVISWNSIINGYVSNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGCAD 308

Query: 108 YGDLPVGKQLHAQAFKLGFSSDLYVLTSLIELYGILDSADTAKWLHDKSTCRNSVSWTIL 167
              + +G+ +H+   K  FS +     +L+++Y      D+AK +  + + R+ VS+T +
Sbjct: 309 SRLISLGRAVHSIGVKACFSREDRFCNTLLDMYSKCGDLDSAKAVFREMSDRSVVSYTSM 368

Query: 168 AKLYLREDKPSFAIDLFYQMVELADDIDSVALATAIGACGALKMLHHGRNIHHLARIHGL 227
              Y RE     A+ LF +M E     D   +   +  C   ++L  G+ +H   + + L
Sbjct: 369 IAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTAVLNCCARYRLLDEGKRVHEWIKENDL 428

Query: 228 EFNILVSNSLLKMYLDCDSIKDARGFFDQMPSKDVISWTELIHMYVKKGGINEAFKLFRQ 287
            F+I VSN+L+ MY  C S+++A   F +M  KD+ISW  +I  Y K    NEA  LF  
Sbjct: 429 GFDIFVSNALMDMYAKCGSMQEAELVFSEMRVKDIISWNTIIGGYSKNCYANEALSLFNL 488

Query: 288 MNMDGELKPDPLTISSILPACGRMAAHKHGKEIHGYVLKNGFDENLIVQNALVDMYVKSG 347
           +  +    PD  T++ +LPAC  ++A   G+EIHGY+++NG+  +  V N+LVDMY K G
Sbjct: 489 LLEEKRFSPDERTVACVLPACASLSAFDKGREIHGYIMRNGYFSDRHVANSLVDMYAKCG 548

Query: 348 CIQSASKTFSMMKEKDMVSWSIMTLGYSLHGQGKLGVGLFREMEKNLKMHRDEITYTAVL 407
            +  A   F  +  KD+VSW++M  GY +HG GK  + LF +M +   +  DEI++ ++L
Sbjct: 549 ALLLAHMLFDDIASKDLVSWTVMIAGYGMHGFGKEAIALFNQM-RQAGIEADEISFVSLL 608

Query: 408 HACTTANMVDEGDFYFSRI-----TKPTVAHIALKVALLARAGRLDEARTFVEKKKLNKH 467
           +AC+ + +VDEG  +F+ +      +PTV H A  V +LAR G L +A  F+E   +   
Sbjct: 609 YACSHSGLVDEGWRFFNIMRHECKIEPTVEHYACIVDMLARTGDLIKAYRFIENMPIPPD 668

Query: 468 PEILRALLDGCRNHRQQKLGKRIIEQLCDLEPLNTENYILLSNWYACNKKWDMVEELRET 527
             I  ALL GCR H   KL +++ E++ +LEP NT  Y+L++N YA  +KW+ V+ LR+ 
Sbjct: 669 ATIWGALLCGCRIHHDVKLAEKVAEKVFELEPENTGYYVLMANIYAEAEKWEQVKRLRKR 728

Query: 528 IRDMGLRPKKAYSWIEFCNKIHVFGTGDVSHPRSQNIYWNLQCLMKKMEEDGSKTNPEFS 587
           I   GLR     SWIE   ++++F  GD S+P ++NI   L+ +  +M E+G     +++
Sbjct: 729 IGQRGLRKNPGCSWIEIKGRVNIFVAGDSSNPETENIEAFLRKVRARMIEEGYSPLTKYA 788

Query: 588 LHDVDE-ERECVPIGHSELLAISFGLISTEAGRTIRITKNLRVCHSCHESAKFISKMVGR 647
           L D +E E+E    GHSE LA++ G+IS+  G+ IR+TKNLRVC  CHE AKF+SK+  R
Sbjct: 789 LIDAEEMEKEEALCGHSEKLAMALGIISSGHGKIIRVTKNLRVCGDCHEMAKFMSKLTRR 848

Query: 648 EIIVKDPYVFHHFKDGCCSCENF 665
           EI+++D   FH FKDG CSC  F
Sbjct: 849 EIVLRDSNRFHQFKDGHCSCRGF 870

BLAST of MELO.jh008690.1 vs. TAIR 10
Match: AT3G12770.1 (mitochondrial editing factor 22 )

HSP 1 Score: 430.3 bits (1105), Expect = 2.9e-120
Identity = 222/631 (35.18%), Postives = 357/631 (56.58%), Query Frame = 0

Query: 41  NYADLCVAHQVFDEIPIWDTFAWNNLIQTHLTNGDWGHVISIYRQMLFRGVRPDKHTLPR 100
           ++ D+  A QVFD++P    F WN +I+ +  N  +   + +Y  M    V PD  T P 
Sbjct: 65  SFGDITFARQVFDDLPRPQIFPWNAIIRGYSRNNHFQDALLMYSNMQLARVSPDSFTFPH 124

Query: 101 IICATRQYGDLPVGKQLHAQAFKLGFSSDLYVLTSLIELYGILDSADTAKWLHDKSTC-- 160
           ++ A      L +G+ +HAQ F+LGF +D++V   LI LY       +A+ + +      
Sbjct: 125 LLKACSGLSHLQMGRFVHAQVFRLGFDADVFVQNGLIALYAKCRRLGSARTVFEGLPLPE 184

Query: 161 RNSVSWTILAKLYLREDKPSFAIDLFYQMVELADDIDSVALATAIGACGALKMLHHGRNI 220
           R  VSWT +   Y +  +P  A+++F QM ++    D VAL + + A   L+ L  GR+I
Sbjct: 185 RTIVSWTAIVSAYAQNGEPMEALEIFSQMRKMDVKPDWVALVSVLNAFTCLQDLKQGRSI 244

Query: 221 HHLARIHGLEFNILVSNSLLKMYLDCDSIKDARGFFDQMPSKDVISWTELIHMYVKKGGI 280
           H      GLE    +  SL  MY  C  +  A+  FD+M S ++I W  +I  Y K G  
Sbjct: 245 HASVVKMGLEIEPDLLISLNTMYAKCGQVATAKILFDKMKSPNLILWNAMISGYAKNGYA 304

Query: 281 NEAFKLFRQMNMDGELKPDPLTISSILPACGRMAAHKHGKEIHGYVLKNGFDENLIVQNA 340
            EA  +F +M ++ +++PD ++I+S + AC ++ + +  + ++ YV ++ + +++ + +A
Sbjct: 305 REAIDMFHEM-INKDVRPDTISITSAISACAQVGSLEQARSMYEYVGRSDYRDDVFISSA 364

Query: 341 LVDMYVKSGCIQSASKTFSMMKEKDMVSWSIMTLGYSLHGQGKLGVGLFREMEKNLKMHR 400
           L+DM+ K G ++ A   F    ++D+V WS M +GY LHG+ +  + L+R ME+   +H 
Sbjct: 365 LIDMFAKCGSVEGARLVFDRTLDRDVVVWSAMIVGYGLHGRAREAISLYRAMERG-GVHP 424

Query: 401 DEITYTAVLHACTTANMVDEGDFYFSRIT----KPTVAHIALKVALLARAGRLDEARTFV 460
           +++T+  +L AC  + MV EG ++F+R+      P   H A  + LL RAG LD+A   +
Sbjct: 425 NDVTFLGLLMACNHSGMVREGWWFFNRMADHKINPQQQHYACVIDLLGRAGHLDQAYEVI 484

Query: 461 EKKKLNKHPEILRALLDGCRNHRQQKLGKRIIEQLCDLEPLNTENYILLSNWYACNKKWD 520
           +   +     +  ALL  C+ HR  +LG+   +QL  ++P NT +Y+ LSN YA  + WD
Sbjct: 485 KCMPVQPGVTVWGALLSACKKHRHVELGEYAAQQLFSIDPSNTGHYVQLSNLYAAARLWD 544

Query: 521 MVEELRETIRDMGLRPKKAYSWIEFCNKIHVFGTGDVSHPRSQNIYWNLQCLMKKMEEDG 580
            V E+R  +++ GL      SW+E   ++  F  GD SHPR + I   ++ +  +++E G
Sbjct: 545 RVAEVRVRMKEKGLNKDVGCSWVEVRGRLEAFRVGDKSHPRYEEIERQVEWIESRLKEGG 604

Query: 581 SKTNPEFSLHDV-DEERECVPIGHSELLAISFGLISTEAGRTIRITKNLRVCHSCHESAK 640
              N + SLHD+ DEE E     HSE +AI++GLIST  G  +RITKNLR C +CH + K
Sbjct: 605 FVANKDASLHDLNDEEAEETLCSHSERIAIAYGLISTPQGTPLRITKNLRACVNCHAATK 664

Query: 641 FISKMVGREIIVKDPYVFHHFKDGCCSCENF 665
            ISK+V REI+V+D   FHHFKDG CSC ++
Sbjct: 665 LISKLVDREIVVRDTNRFHHFKDGVCSCGDY 693

BLAST of MELO.jh008690.1 vs. TAIR 10
Match: AT1G15510.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 392.9 bits (1008), Expect = 5.2e-109
Identity = 229/623 (36.76%), Postives = 338/623 (54.25%), Query Frame = 0

Query: 44  DLCVAHQVFDEIPIWDTFAWNNLIQTHLTNGDWGHVISIYRQMLFRGVRPDKHTLPRIIC 103
           D+  A  +FD +P  D  +WN +I  +  NG     + ++  M    V PD  TL  +I 
Sbjct: 246 DVKSARLLFDRMPRRDIISWNAMISGYFENGMCHEGLELFFAMRGLSVDPDLMTLTSVIS 305

Query: 104 ATRQYGDLPVGKQLHAQAFKLGFSSDLYVLTSLIELYGILDSADTAKWLHDKSTCRNSVS 163
           A    GD  +G+ +HA     GF+ D+ V  SL ++Y    S   A+ L  +   ++ VS
Sbjct: 306 ACELLGDRRLGRDIHAYVITTGFAVDISVCNSLTQMYLNAGSWREAEKLFSRMERKDIVS 365

Query: 164 WTILAKLYLREDKPSFAIDLFYQMVELADDIDSVALATAIGACGALKMLHHGRNIHHLAR 223
           WT +   Y     P  AID +  M + +   D + +A  + AC  L  L  G  +H LA 
Sbjct: 366 WTTMISGYEYNFLPDKAIDTYRMMDQDSVKPDEITVAAVLSACATLGDLDTGVELHKLAI 425

Query: 224 IHGLEFNILVSNSLLKMYLDCDSIKDARGFFDQMPSKDVISWTELIHMYVKKGGINEAFK 283
              L   ++V+N+L+ MY  C  I  A   F  +P K+VISWT +I          EA  
Sbjct: 426 KARLISYVIVANNLINMYSKCKCIDKALDIFHNIPRKNVISWTSIIAGLRLNNRCFEALI 485

Query: 284 LFRQMNMDGELKPDPLTISSILPACGRMAAHKHGKEIHGYVLKNGFDENLIVQNALVDMY 343
             RQM M   L+P+ +T+++ L AC R+ A   GKEIH +VL+ G   +  + NAL+DMY
Sbjct: 486 FLRQMKM--TLQPNAITLTAALAACARIGALMCGKEIHAHVLRTGVGLDDFLPNALLDMY 545

Query: 344 VKSGCIQSASKTFSMMKEKDMVSWSIMTLGYSLHGQGKLGVGLFREMEKNLKMHRDEITY 403
           V+ G + +A   F+  K KD+ SW+I+  GYS  GQG + V LF  M K+ ++  DEIT+
Sbjct: 546 VRCGRMNTAWSQFNSQK-KDVTSWNILLTGYSERGQGSMVVELFDRMVKS-RVRPDEITF 605

Query: 404 TAVLHACTTANMVDEGDFYFSRI----TKPTVAHIALKVALLARAGRLDEARTFVEKKKL 463
            ++L  C+ + MV +G  YFS++      P + H A  V LL RAG L EA  F++K  +
Sbjct: 606 ISLLCGCSKSQMVRQGLMYFSKMEDYGVTPNLKHYACVVDLLGRAGELQEAHKFIQKMPV 665

Query: 464 NKHPEILRALLDGCRNHRQQKLGKRIIEQLCDLEPLNTENYILLSNWYACNKKWDMVEEL 523
              P +  ALL+ CR H +  LG+   + + +L+  +   YILL N YA   KW  V ++
Sbjct: 666 TPDPAVWGALLNACRIHHKIDLGELSAQHIFELDKKSVGYYILLCNLYADCGKWREVAKV 725

Query: 524 RETIRDMGLRPKKAYSWIEFCNKIHVFGTGDVSHPRSQNIYWNLQCLMKKMEEDG-SKTN 583
           R  +++ GL      SW+E   K+H F + D  HP+++ I   L+   +KM E G +K +
Sbjct: 726 RRMMKENGLTVDAGCSWVEVKGKVHAFLSDDKYHPQTKEINTVLEGFYEKMSEVGLTKIS 785

Query: 584 PEFSLHDVDEERECVPIGHSELLAISFGLISTEAGRTIRITKNLRVCHSCHESAKFISKM 643
              S+ + +  R+ +  GHSE  AI+FGLI+T  G  I +TKNL +C +CH++ KFISK 
Sbjct: 786 ESSSMDETEISRDEIFCGHSERKAIAFGLINTVPGMPIWVTKNLSMCENCHDTVKFISKT 845

Query: 644 VGREIIVKDPYVFHHFKDGCCSC 662
           V REI V+D   FHHFKDG CSC
Sbjct: 846 VRREISVRDAEHFHHFKDGECSC 864

BLAST of MELO.jh008690.1 vs. TAIR 10
Match: AT2G22070.1 (pentatricopeptide (PPR) repeat-containing protein )

HSP 1 Score: 389.0 bits (998), Expect = 7.4e-108
Identity = 234/731 (32.01%), Postives = 371/731 (50.75%), Query Frame = 0

Query: 14  ITQKPYHAYHRHPPFNNLPHVRT-----TTVENYA---DLCVAHQVFDEIPIWDTFAWNN 73
           +  K  +A H    F+ +P +RT     T +  Y+   D+    + FD++P  D+ +W  
Sbjct: 58  VYSKTGYALHARKLFDEMP-LRTAFSWNTVLSAYSKRGDMDSTCEFFDQLPQRDSVSWTT 117

Query: 74  LIQTHLTNGDWGHVISIYRQMLFRGVRPDKHTLPRIICATRQYGDLPVGKQLHAQAFKLG 133
           +I  +   G +   I +   M+  G+ P + TL  ++ +      +  GK++H+   KLG
Sbjct: 118 MIVGYKNIGQYHKAIRVMGDMVKEGIEPTQFTLTNVLASVAATRCMETGKKVHSFIVKLG 177

Query: 134 FSSDLYVLTSLIELYGILDSADTAKWLHDKSTCRNSVSWTILAKLYLREDKPSFAIDLFY 193
              ++ V  SL+ +Y        AK++ D+   R+  SW  +  L+++  +   A+  F 
Sbjct: 178 LRGNVSVSNSLLNMYAKCGDPMMAKFVFDRMVVRDISSWNAMIALHMQVGQMDLAMAQFE 237

Query: 194 QMVE-----------------------------LADDI---DSVALATAIGACGALKMLH 253
           QM E                             L D +   D   LA+ + AC  L+ L 
Sbjct: 238 QMAERDIVTWNSMISGFNQRGYDLRALDIFSKMLRDSLLSPDRFTLASVLSACANLEKLC 297

Query: 254 HGRNIHHLARIHGLEFNILVSNSLLKMYLDCDSIKDARGFFDQ----------------- 313
            G+ IH      G + + +V N+L+ MY  C  ++ AR   +Q                 
Sbjct: 298 IGKQIHSHIVTTGFDISGIVLNALISMYSRCGGVETARRLIEQRGTKDLKIEGFTALLDG 357

Query: 314 ----------------MPSKDVISWTELIHMYVKKGGINEAFKLFRQMNMDGELKPDPLT 373
                           +  +DV++WT +I  Y + G   EA  LFR M + G  +P+  T
Sbjct: 358 YIKLGDMNQAKNIFVSLKDRDVVAWTAMIVGYEQHGSYGEAINLFRSM-VGGGQRPNSYT 417

Query: 374 ISSILPACGRMAAHKHGKEIHGYVLKNGFDENLIVQNALVDMYVKSGCIQSASKTFSMMK 433
           ++++L     +A+  HGK+IHG  +K+G   ++ V NAL+ MY K+G I SAS+ F +++
Sbjct: 418 LAAMLSVASSLASLSHGKQIHGSAVKSGEIYSVSVSNALITMYAKAGNITSASRAFDLIR 477

Query: 434 -EKDMVSWSIMTLGYSLHGQGKLGVGLFREMEKNLKMHRDEITYTAVLHACTTANMVDEG 493
            E+D VSW+ M +  + HG  +  + LF  M     +  D ITY  V  ACT A +V++G
Sbjct: 478 CERDTVSWTSMIIALAQHGHAEEALELFETMLME-GLRPDHITYVGVFSACTHAGLVNQG 537

Query: 494 DFYFSRITK-----PTVAHIALKVALLARAGRLDEARTFVEKKKLNKHPEILRALLDGCR 553
             YF  +       PT++H A  V L  RAG L EA+ F+EK  +        +LL  CR
Sbjct: 538 RQYFDMMKDVDKIIPTLSHYACMVDLFGRAGLLQEAQEFIEKMPIEPDVVTWGSLLSACR 597

Query: 554 NHRQQKLGKRIIEQLCDLEPLNTENYILLSNWYACNKKWDMVEELRETIRDMGLRPKKAY 613
            H+   LGK   E+L  LEP N+  Y  L+N Y+   KW+   ++R++++D  ++ ++ +
Sbjct: 598 VHKNIDLGKVAAERLLLLEPENSGAYSALANLYSACGKWEEAAKIRKSMKDGRVKKEQGF 657

Query: 614 SWIEFCNKIHVFGTGDVSHPRSQNIYWNLQCLMKKMEEDGSKTNPEFSLHDVDEE-RECV 665
           SWIE  +K+HVFG  D +HP    IY  ++ +  ++++ G   +    LHD++EE +E +
Sbjct: 658 SWIEVKHKVHVFGVEDGTHPEKNEIYMTMKKIWDEIKKMGYVPDTASVLHDLEEEVKEQI 717

BLAST of MELO.jh008690.1 vs. TAIR 10
Match: AT1G11290.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 386.3 bits (991), Expect = 4.8e-107
Identity = 210/622 (33.76%), Postives = 339/622 (54.50%), Query Frame = 0

Query: 48  AHQVFDEIPIWDTFAWNNLIQTHLTNGDWGHVISIYRQMLFRGVRPDKHTLPRIICATRQ 107
           A +VFD +P  D  +WN ++  +  NG     + + + M    ++P   T+  ++ A   
Sbjct: 189 ARKVFDRMPERDLVSWNTIVAGYSQNGMARMALEMVKSMCEENLKPSFITIVSVLPAVSA 248

Query: 108 YGDLPVGKQLHAQAFKLGFSSDLYVLTSLIELYGILDSADTAKWLHDKSTCRNSVSWTIL 167
              + VGK++H  A + GF S + + T+L+++Y    S +TA+ L D    RN VSW  +
Sbjct: 249 LRLISVGKEIHGYAMRSGFDSLVNISTALVDMYAKCGSLETARQLFDGMLERNVVSWNSM 308

Query: 168 AKLYLREDKPSFAIDLFYQMVELADDIDSVALATAIGACGALKMLHHGRNIHHLARIHGL 227
              Y++ + P  A+ +F +M++       V++  A+ AC  L  L  GR IH L+   GL
Sbjct: 309 IDAYVQNENPKEAMLIFQKMLDEGVKPTDVSVMGALHACADLGDLERGRFIHKLSVELGL 368

Query: 228 EFNILVSNSLLKMYLDCDSIKDARGFFDQMPSKDVISWTELIHMYVKKGGINEAFKLFRQ 287
           + N+ V NSL+ MY  C  +  A   F ++ S+ ++SW  +I  + + G   +A   F Q
Sbjct: 369 DRNVSVVNSLISMYCKCKEVDTAASMFGKLQSRTLVSWNAMILGFAQNGRPIDALNYFSQ 428

Query: 288 MNMDGELKPDPLTISSILPACGRMAAHKHGKEIHGYVLKNGFDENLIVQNALVDMYVKSG 347
           M     +KPD  T  S++ A   ++   H K IHG V+++  D+N+ V  ALVDMY K G
Sbjct: 429 MR-SRTVKPDTFTYVSVITAIAELSITHHAKWIHGVVMRSCLDKNVFVTTALVDMYAKCG 488

Query: 348 CIQSASKTFSMMKEKDMVSWSIMTLGYSLHGQGKLGVGLFREMEKNLKMHRDEITYTAVL 407
            I  A   F MM E+ + +W+ M  GY  HG GK  + LF EM+K   +  + +T+ +V+
Sbjct: 489 AIMIARLIFDMMSERHVTTWNAMIDGYGTHGFGKAALELFEEMQKG-TIKPNGVTFLSVI 548

Query: 408 HACTTANMVDEG--DFYFSR---ITKPTVAHIALKVALLARAGRLDEARTFVEKKKLNKH 467
            AC+ + +V+ G   FY  +     + ++ H    V LL RAGRL+EA  F+ +  +   
Sbjct: 549 SACSHSGLVEAGLKCFYMMKENYSIELSMDHYGAMVDLLGRAGRLNEAWDFIMQMPVKPA 608

Query: 468 PEILRALLDGCRNHRQQKLGKRIIEQLCDLEPLNTENYILLSNWYACNKKWDMVEELRET 527
             +  A+L  C+ H+     ++  E+L +L P +   ++LL+N Y     W+ V ++R +
Sbjct: 609 VNVYGAMLGACQIHKNVNFAEKAAERLFELNPDDGGYHVLLANIYRAASMWEKVGQVRVS 668

Query: 528 IRDMGLRPKKAYSWIEFCNKIHVFGTGDVSHPRSQNIYWNLQCLMKKMEEDGSKTNPEFS 587
           +   GLR     S +E  N++H F +G  +HP S+ IY  L+ L+  ++E G   +    
Sbjct: 669 MLRQGLRKTPGCSMVEIKNEVHSFFSGSTAHPDSKKIYAFLEKLICHIKEAGYVPDTNLV 728

Query: 588 LHDVDEERECVPIGHSELLAISFGLISTEAGRTIRITKNLRVCHSCHESAKFISKMVGRE 647
           L   ++ +E +   HSE LAISFGL++T AG TI + KNLRVC  CH + K+IS + GRE
Sbjct: 729 LGVENDVKEQLLSTHSEKLAISFGLLNTTAGTTIHVRKNLRVCADCHNATKYISLVTGRE 788

Query: 648 IIVKDPYVFHHFKDGCCSCENF 665
           I+V+D   FHHFK+G CSC ++
Sbjct: 789 IVVRDMQRFHHFKNGACSCGDY 808

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008465161.10.0100.00PREDICTED: pentatricopeptide repeat-containing protein DOT4, chloroplastic-like ... [more]
XP_004137884.20.095.49pentatricopeptide repeat-containing protein DOT4, chloroplastic [Cucumis sativus... [more]
XP_038905218.10.089.79pentatricopeptide repeat-containing protein DOT4, chloroplastic-like isoform X1 ... [more]
KAA0037696.10.0100.00pentatricopeptide repeat-containing protein DOT4 [Cucumis melo var. makuwa][more]
TYK10677.10.099.83pentatricopeptide repeat-containing protein DOT4 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q9SN395.2e-12236.92Pentatricopeptide repeat-containing protein DOT4, chloroplastic OS=Arabidopsis t... [more]
Q9LTV84.1e-11935.18Pentatricopeptide repeat-containing protein At3g12770 OS=Arabidopsis thaliana OX... [more]
Q9M9E27.3e-10836.76Pentatricopeptide repeat-containing protein At1g15510, chloroplastic OS=Arabidop... [more]
Q9SHZ81.0e-10632.01Pentatricopeptide repeat-containing protein At2g22070 OS=Arabidopsis thaliana OX... [more]
Q3E6Q16.8e-10633.76Pentatricopeptide repeat-containing protein At1g11290, chloroplastic OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A1S3CPR50.0100.00pentatricopeptide repeat-containing protein DOT4, chloroplastic-like OS=Cucumis ... [more]
A0A0A0L9N40.095.03DYW_deaminase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G7228... [more]
A0A5A7T6A70.0100.00Pentatricopeptide repeat-containing protein DOT4 OS=Cucumis melo var. makuwa OX=... [more]
A0A5D3CFC20.099.83Pentatricopeptide repeat-containing protein DOT4 OS=Cucumis melo var. makuwa OX=... [more]
A0A6J1I9E10.082.11pentatricopeptide repeat-containing protein DOT4, chloroplastic-like OS=Cucurbit... [more]
Match NameE-valueIdentityDescription
AT4G18750.13.7e-12336.92Pentatricopeptide repeat (PPR) superfamily protein [more]
AT3G12770.12.9e-12035.18mitochondrial editing factor 22 [more]
AT1G15510.15.2e-10936.76Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT2G22070.17.4e-10832.01pentatricopeptide (PPR) repeat-containing protein [more]
AT1G11290.14.8e-10733.76Pentatricopeptide repeat (PPR) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 314..598
e-value: 3.1E-17
score: 65.0
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 34..168
e-value: 1.8E-10
score: 42.7
coord: 169..313
e-value: 5.4E-24
score: 87.1
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 62..94
e-value: 1.6E-4
score: 19.6
coord: 263..297
e-value: 0.0015
score: 16.6
coord: 335..365
e-value: 0.0029
score: 15.7
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 260..308
e-value: 5.7E-9
score: 36.1
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 162..189
e-value: 0.015
score: 15.6
coord: 62..91
e-value: 0.17
score: 12.2
coord: 365..393
e-value: 0.025
score: 14.8
coord: 337..363
e-value: 0.011
score: 15.9
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 332..366
score: 8.53891
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 261..295
score: 9.96388
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 59..93
score: 10.577712
IPR032867DYW domainPFAMPF14432DYW_deaminasecoord: 534..655
e-value: 5.0E-32
score: 110.4
NoneNo IPR availablePANTHERPTHR24015OS07G0578800 PROTEIN-RELATEDcoord: 1..658
NoneNo IPR availablePANTHERPTHR24015:SF1853REPEAT-CONTAINING PROTEIN, PUTATIVE-RELATEDcoord: 1..658

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO.jh008690.1.t1MELO.jh008690.1.t1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding
molecular_function GO:0008270 zinc ion binding