MELO.jh003103.1 (gene) Melon (Harukei-3) v1.41

Overview


Name	MELO.jh003103.1
Type	gene
Organism	Cucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
Description	(1->3)-beta-glucan endohydrolase
Location	chr02: 2641046 .. 2642542 (-)
RNA-Seq Expression	MELO.jh003103.1
Synteny	MELO.jh003103.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDSstart_codonstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTGTGCCAACATTTTAAAAACCCCTTTTCTTGTGTTGTTTCTTTGTAAAATTTTCTTTAGAACATTCTTAAAAAAAAAAATGGCGAAACTTGTCCAAATAATTGTCTTCTTAATGGCATCGATATTAATGCCCACTAGCTTGGTCAGAGCACACGATGTTCTTCTCGGCGCCTACTACGGTCTTAGTGGCAACAACTTGCCTCCACCATGGAAGGTGGTGCATCTCTGCGAAAAATACAACATTCGTCGGATCAGATTAGATATACCAACCGTGGATGTTTTGACTGCGTTTCGTGGTTCGAATATTGATCTCTCTTTTAGTGTACCAAATGACATGCTAACGGATATGGCCACAAATAAAGCTCTTGTAGAAGAGTGGTTTAATACTTACATTAAACCCTTTGTTGACGATTTCAAAATCAATTACATCATTGTTGGGGACAAGGCCGTCCCTGGCCTTGACAATTTCATATTGCCAGTCATGATGTCCCTTCAAGATCTCCTCAATGCAAACTATTTTGGACAAGTGAAGCTTACTACATTGGTCGGGTACGGCTAAACTAACGTCATAAGTTCAAAAGGTTAAGTCAATAGATATATTAAATGATCACCAACTAACCCAAAAGTTGATACAGTAAGTTGAGGTATGTTAAATCAAATTCATGGCCAAATAAGAGAAGTTCATCCCTCATTTGAGAAAACTCTTCGAGAGGACTAAAGTTAATACAATAAGTTTTTCTTAAGCTGAGGAGTGAAAGCAAATTTAATATTGTATCATTGAAAAGTAATTTTATTGGTATTGTTGACAGGTACAATGCTGCATTGGTCTCTCAAGACCCGCCATCCTCCGGCGTATTCAACCCAACCGTAAGGGAGAACATGAGAGGCATACTCAAATTCTTAGCAGAAGAAGGCTCAGCTTTGATGGTTAGCGTATTCCCGCATCGACAATATAAGTCCAATGGCGGTATGAGTTTGAACTACGCAGCATTAAAGGAGACAAAGCCAGTAGTTGAAGACGGAGAGTTGAAATATTCCAACTTGTTCGATGCAATGATGGATGCATTTTATGCAGCTATCGAAAAAGAAACGATTGGAGAAGTGACTCTTGTAGTTGGAGAAACTGGGTGGCCAACTTGCGGAGACAATCATGAAATTGAAACACTCGATATTGCCAAGCAGTACAACAACAAATTTAAGAGTCATATTTCCTCTGGCAAGGGAACTCCAAGGAAGCCAAATGTTTATCTTGAAGGTTTCATACAAAGTTTATTCAATGAAAATCAAAAACCTGAAGGAGAGTCACAATGCTTTGGGGTGTTTGATGTCAACATGAACCCTATTTATTCATTGTTTTGGTAGTTTGCTGCCCAAAAATTATTTAGAATTATAAATTTAATATTTACTACGATTTTTCCTATTTGACGTTTATCGTGTTCAACCCAAACTAAAATAATTTGATCAAACTATAATAACATAGAACCTGTAATAACTCA

mRNA sequence

TTTGTGCCAACATTTTAAAAACCCCTTTTCTTGTGTTGTTTCTTTGTAAAATTTTCTTTAGAACATTCTTAAAAAAAAAAATGGCGAAACTTGTCCAAATAATTGTCTTCTTAATGGCATCGATATTAATGCCCACTAGCTTGGTCAGAGCACACGATGTTCTTCTCGGCGCCTACTACGGTCTTAGTGGCAACAACTTGCCTCCACCATGGAAGGTGGTGCATCTCTGCGAAAAATACAACATTCGTCGGATCAGATTAGATATACCAACCGTGGATGTTTTGACTGCGTTTCGTGGTTCGAATATTGATCTCTCTTTTAGTGTACCAAATGACATGCTAACGGATATGGCCACAAATAAAGCTCTTGTAGAAGAGTGGTTTAATACTTACATTAAACCCTTTGTTGACGATTTCAAAATCAATTACATCATTGTTGGGGACAAGGCCGTCCCTGGCCTTGACAATTTCATATTGCCAGTCATGATGTCCCTTCAAGATCTCCTCAATGCAAACTATTTTGGACAAGTGAAGCTTACTACATTGGTCGGGTACAATGCTGCATTGGTCTCTCAAGACCCGCCATCCTCCGGCGTATTCAACCCAACCGTAAGGGAGAACATGAGAGGCATACTCAAATTCTTAGCAGAAGAAGGCTCAGCTTTGATGGTTAGCGTATTCCCGCATCGACAATATAAGTCCAATGGCGGTATGAGTTTGAACTACGCAGCATTAAAGGAGACAAAGCCAGTAGTTGAAGACGGAGAGTTGAAATATTCCAACTTGTTCGATGCAATGATGGATGCATTTTATGCAGCTATCGAAAAAGAAACGATTGGAGAAGTGACTCTTGTAGTTGGAGAAACTGGGTGGCCAACTTGCGGAGACAATCATGAAATTGAAACACTCGATATTGCCAAGCAGTACAACAACAAATTTAAGAGTCATATTTCCTCTGGCAAGGGAACTCCAAGGAAGCCAAATGTTTATCTTGAAGGTTTCATACAAAGTTTATTCAATGAAAATCAAAAACCTGAAGGAGAGTCACAATGCTTTGGGGTGTTTGATGTCAACATGAACCCTATTTATTCATTGTTTTGGTAGTTTGCTGCCCAAAAATTATTTAGAATTATAAATTTAATATTTACTACGATTTTTCCTATTTGACGTTTATCGTGTTCAACCCAAACTAAAATAATTTGATCAAACTATAATAACATAGAACCTGTAATAACTCA

Coding sequence (CDS)

ATGGCGAAACTTGTCCAAATAATTGTCTTCTTAATGGCATCGATATTAATGCCCACTAGCTTGGTCAGAGCACACGATGTTCTTCTCGGCGCCTACTACGGTCTTAGTGGCAACAACTTGCCTCCACCATGGAAGGTGGTGCATCTCTGCGAAAAATACAACATTCGTCGGATCAGATTAGATATACCAACCGTGGATGTTTTGACTGCGTTTCGTGGTTCGAATATTGATCTCTCTTTTAGTGTACCAAATGACATGCTAACGGATATGGCCACAAATAAAGCTCTTGTAGAAGAGTGGTTTAATACTTACATTAAACCCTTTGTTGACGATTTCAAAATCAATTACATCATTGTTGGGGACAAGGCCGTCCCTGGCCTTGACAATTTCATATTGCCAGTCATGATGTCCCTTCAAGATCTCCTCAATGCAAACTATTTTGGACAAGTGAAGCTTACTACATTGGTCGGGTACAATGCTGCATTGGTCTCTCAAGACCCGCCATCCTCCGGCGTATTCAACCCAACCGTAAGGGAGAACATGAGAGGCATACTCAAATTCTTAGCAGAAGAAGGCTCAGCTTTGATGGTTAGCGTATTCCCGCATCGACAATATAAGTCCAATGGCGGTATGAGTTTGAACTACGCAGCATTAAAGGAGACAAAGCCAGTAGTTGAAGACGGAGAGTTGAAATATTCCAACTTGTTCGATGCAATGATGGATGCATTTTATGCAGCTATCGAAAAAGAAACGATTGGAGAAGTGACTCTTGTAGTTGGAGAAACTGGGTGGCCAACTTGCGGAGACAATCATGAAATTGAAACACTCGATATTGCCAAGCAGTACAACAACAAATTTAAGAGTCATATTTCCTCTGGCAAGGGAACTCCAAGGAAGCCAAATGTTTATCTTGAAGGTTTCATACAAAGTTTATTCAATGAAAATCAAAAACCTGAAGGAGAGTCACAATGCTTTGGGGTGTTTGATGTCAACATGAACCCTATTTATTCATTGTTTTGGTAG

Protein sequence

MAKLVQIIVFLMASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRLDIPTVDVLTAFRGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVGDKAVPGLDNFILPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVRENMRGILKFLAEEGSALMVSVFPHRQYKSNGGMSLNYAALKETKPVVEDGELKYSNLFDAMMDAFYAAIEKETIGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKPNVYLEGFIQSLFNENQKPEGESQCFGVFDVNMNPIYSLFW

Homology

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BLAST of MELO.jh003103.1 vs. NCBI nr
Match: XP_008451156.1 (PREDICTED: probable glucan endo-1,3-beta-glucosidase BG5 [Cucumis melo])

HSP 1 Score: 689 bits (1777), Expect = 4.22e-250
Identity = 340/340 (100.00%), Postives = 340/340 (100.00%), Query Frame = 0


Query: 1   MAKLVQIIVFLMASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRL 60
           MAKLVQIIVFLMASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRL
Sbjct: 1   MAKLVQIIVFLMASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRL 60

Query: 61  DIPTVDVLTAFRGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVG 120
           DIPTVDVLTAFRGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVG
Sbjct: 61  DIPTVDVLTAFRGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVG 120

Query: 121 DKAVPGLDNFILPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVREN 180
           DKAVPGLDNFILPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVREN
Sbjct: 121 DKAVPGLDNFILPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVREN 180

Query: 181 MRGILKFLAEEGSALMVSVFPHRQYKSNGGMSLNYAALKETKPVVEDGELKYSNLFDAMM 240
           MRGILKFLAEEGSALMVSVFPHRQYKSNGGMSLNYAALKETKPVVEDGELKYSNLFDAMM
Sbjct: 181 MRGILKFLAEEGSALMVSVFPHRQYKSNGGMSLNYAALKETKPVVEDGELKYSNLFDAMM 240

Query: 241 DAFYAAIEKETIGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKP 300
           DAFYAAIEKETIGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKP
Sbjct: 241 DAFYAAIEKETIGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKP 300

Query: 301 NVYLEGFIQSLFNENQKPEGESQCFGVFDVNMNPIYSLFW 340
           NVYLEGFIQSLFNENQKPEGESQCFGVFDVNMNPIYSLFW
Sbjct: 301 NVYLEGFIQSLFNENQKPEGESQCFGVFDVNMNPIYSLFW 340

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BLAST of MELO.jh003103.1 vs. NCBI nr
Match: KAA0055784.1 (putative glucan endo-1,3-beta-glucosidase BG5 [Cucumis melo var. makuwa] >TYK10035.1 putative glucan endo-1,3-beta-glucosidase BG5 [Cucumis melo var. makuwa])

HSP 1 Score: 672 bits (1734), Expect = 1.00e-243
Identity = 329/329 (100.00%), Postives = 329/329 (100.00%), Query Frame = 0


Query: 12  MASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRLDIPTVDVLTAF 71
           MASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRLDIPTVDVLTAF
Sbjct: 1   MASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRLDIPTVDVLTAF 60

Query: 72  RGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVGDKAVPGLDNFI 131
           RGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVGDKAVPGLDNFI
Sbjct: 61  RGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVGDKAVPGLDNFI 120

Query: 132 LPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVRENMRGILKFLAEE 191
           LPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVRENMRGILKFLAEE
Sbjct: 121 LPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVRENMRGILKFLAEE 180

Query: 192 GSALMVSVFPHRQYKSNGGMSLNYAALKETKPVVEDGELKYSNLFDAMMDAFYAAIEKET 251
           GSALMVSVFPHRQYKSNGGMSLNYAALKETKPVVEDGELKYSNLFDAMMDAFYAAIEKET
Sbjct: 181 GSALMVSVFPHRQYKSNGGMSLNYAALKETKPVVEDGELKYSNLFDAMMDAFYAAIEKET 240

Query: 252 IGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKPNVYLEGFIQSL 311
           IGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKPNVYLEGFIQSL
Sbjct: 241 IGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKPNVYLEGFIQSL 300

Query: 312 FNENQKPEGESQCFGVFDVNMNPIYSLFW 340
           FNENQKPEGESQCFGVFDVNMNPIYSLFW
Sbjct: 301 FNENQKPEGESQCFGVFDVNMNPIYSLFW 329

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BLAST of MELO.jh003103.1 vs. NCBI nr
Match: XP_004144124.2 (probable glucan endo-1,3-beta-glucosidase BG4 [Cucumis sativus])

HSP 1 Score: 544 bits (1401), Expect = 1.24e-192
Identity = 265/339 (78.17%), Postives = 301/339 (88.79%), Query Frame = 0


Query: 1   MAKLVQIIVFLMASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRL 60
           MAK+VQI VFLMASILM  ++V A+D+LLGAYYGL G+NLPPPWKVV LCE+YNIRR+RL
Sbjct: 1   MAKVVQI-VFLMASILMAATVVGAYDILLGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRL 60

Query: 61  DIPTVDVLTAFRGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVG 120
           D+P VDVLTAFRG  ID+SF +PN+MLTD+A NK LVEEWFNTY+K FVDDF+INYIIVG
Sbjct: 61  DVPDVDVLTAFRGGAIDVSFGIPNNMLTDLANNKGLVEEWFNTYVKTFVDDFRINYIIVG 120

Query: 121 DKAVPGLDNFILPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVREN 180
           DKA+P   NFILP+MMSLQDLLNANY GQVKLTTLVGYNAALVS+DPPSSG F+PTVRE+
Sbjct: 121 DKAIPSHANFILPIMMSLQDLLNANYLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRES 180

Query: 181 MRGILKFLAEEGSALMVSVFPHRQYKSNGGMSLNYAALKETKPVVEDGELKYSNLFDAMM 240
           MRGILKFLAEEGSALMVSVFPH++Y+ NGG+SLNYA   ET PVV+D  L+Y NLFDAM+
Sbjct: 181 MRGILKFLAEEGSALMVSVFPHQKYEFNGGISLNYAIFNETNPVVQDEGLEYYNLFDAMV 240

Query: 241 DAFYAAIEKETIGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKP 300
           DAFYAAIEKE +GEV +VVGETGWPTCGD     T  IAK+YNNKFKSHISSG GTPRKP
Sbjct: 241 DAFYAAIEKEMVGEVNIVVGETGWPTCGDISG--TQAIAKEYNNKFKSHISSGNGTPRKP 300

Query: 301 NVYLEGFIQSLFNENQKPEGESQCFGVFDVNMNPIYSLF 339
           NVYLEGFIQS+FNE++KPEG+SQC+G+FDVNM PIY LF
Sbjct: 301 NVYLEGFIQSIFNEDKKPEGDSQCYGMFDVNMKPIYPLF 336

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BLAST of MELO.jh003103.1 vs. NCBI nr
Match: KAE8653445.1 (hypothetical protein Csa_006879 [Cucumis sativus])

HSP 1 Score: 540 bits (1391), Expect = 4.62e-186
Identity = 260/332 (78.31%), Postives = 295/332 (88.86%), Query Frame = 0


Query: 8   IVFLMASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRLDIPTVDV 67
           IVFLMASILM  ++V A+D+LLGAYYGL G+NLPPPWKVV LCE+YNIRR+RLD+P VDV
Sbjct: 345 IVFLMASILMAATVVGAYDILLGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDVPDVDV 404

Query: 68  LTAFRGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVGDKAVPGL 127
           LTAFRG  ID+SF +PN+MLTD+A NK LVEEWFNTY+K FVDDF+INYIIVGDKA+P  
Sbjct: 405 LTAFRGGAIDVSFGIPNNMLTDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSH 464

Query: 128 DNFILPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVRENMRGILKF 187
            NFILP+MMSLQDLLNANY GQVKLTTLVGYNAALVS+DPPSSG F+PTVRE+MRGILKF
Sbjct: 465 ANFILPIMMSLQDLLNANYLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKF 524

Query: 188 LAEEGSALMVSVFPHRQYKSNGGMSLNYAALKETKPVVEDGELKYSNLFDAMMDAFYAAI 247
           LAEEGSALMVSVFPH++Y+ NGG+SLNYA   ET PVV+D  L+Y NLFDAM+DAFYAAI
Sbjct: 525 LAEEGSALMVSVFPHQKYEFNGGISLNYAIFNETNPVVQDEGLEYYNLFDAMVDAFYAAI 584

Query: 248 EKETIGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKPNVYLEGF 307
           EKE +GEV +VVGETGWPTCGD     T  IAK+YNNKFKSHISSG GTPRKPNVYLEGF
Sbjct: 585 EKEMVGEVNIVVGETGWPTCGDISG--TQAIAKEYNNKFKSHISSGNGTPRKPNVYLEGF 644

Query: 308 IQSLFNENQKPEGESQCFGVFDVNMNPIYSLF 339
           IQS+FNE++KPEG+SQC+G+FDVNM PIY LF
Sbjct: 645 IQSIFNEDKKPEGDSQCYGMFDVNMKPIYPLF 674

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BLAST of MELO.jh003103.1 vs. NCBI nr
Match: XP_038880473.1 (probable glucan endo-1,3-beta-glucosidase BG5 [Benincasa hispida])

HSP 1 Score: 524 bits (1350), Expect = 4.82e-185
Identity = 253/338 (74.85%), Postives = 297/338 (87.87%), Query Frame = 0


Query: 1   MAKLVQIIVFLMASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRL 60
           MAKL QII FLMASI+M  ++VRA++VLLG   GLSG+NLPPPWK+V LC+KY+IRRIRL
Sbjct: 1   MAKLAQII-FLMASIIMAATIVRAYNVLLGVCLGLSGDNLPPPWKMVQLCKKYSIRRIRL 60

Query: 61  DIPTVDVLTAFRGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVG 120
           D P +DVL AFRG+ IDLSF +PNDMLTDMATNKALVEEWFNTYIKPFV++FKINY+IVG
Sbjct: 61  DEPNIDVLNAFRGAEIDLSFGIPNDMLTDMATNKALVEEWFNTYIKPFVNEFKINYVIVG 120

Query: 121 DKAVPGLDNFILPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVREN 180
           DKA+PGLD  ILPVMMS QDLLNANY GQVKLTTLVGYNAAL  +DPPSSG F+PT+ EN
Sbjct: 121 DKAIPGLDICILPVMMSFQDLLNANYIGQVKLTTLVGYNAALAYKDPPSSGAFHPTISEN 180

Query: 181 MRGILKFLAEEGSALMVSVFPHRQYKSNGGMSLNYAALKETKPVVEDGELKYSNLFDAMM 240
           MRGILKFL+EEGS LMVSVFP+R+Y  +GG+SLNYA   ET+PVV DGEL Y NLFDAM+
Sbjct: 181 MRGILKFLSEEGSPLMVSVFPYRKYMFDGGISLNYAIFNETEPVVRDGELNYYNLFDAMV 240

Query: 241 DAFYAAIEKETIGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKP 300
           +A YAAI+KE +G+VT+VVGETGWPTCG + +  T  IA++YN KF SHI SGKGTPRKP
Sbjct: 241 EAVYAAIDKEAVGDVTVVVGETGWPTCGSDDDA-TPAIAEEYNKKFVSHICSGKGTPRKP 300

Query: 301 NVYLEGFIQSLFNENQKPEGESQCFGVFDVNMNPIYSL 338
           NV+LEGFIQS+FNE++KP+G+SQC+G+FD+NMNPIYSL
Sbjct: 301 NVFLEGFIQSIFNEDEKPQGDSQCYGMFDINMNPIYSL 336

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BLAST of MELO.jh003103.1 vs. ExPASy Swiss-Prot
Match: O49353 (Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN=BG5 PE=2 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 6.2e-63
Identity = 130/315 (41.27%), Postives = 195/315 (61.90%), Query Frame = 0


Query: 29  LGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRLDIPTVDVLTAFRG-SNIDLSFSVPNDML 88
           +G  YGL G+NLP P KV+ L +  +I +IR+  P  +VL A RG  +I ++  V +  L
Sbjct: 43  IGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQDL 102

Query: 89  TDMATNKALVEEWFNTYIKPFVDDFKINYIIVGDKAVPG-LDNFILPVMMSLQDLLNANY 148
             ++ ++  V+ WF T I+P++ D  I +I VG++ +PG +   +LPVM SL +L+ +  
Sbjct: 103 AALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRN 162

Query: 149 FGQVKLTTLVGYNAALVSQDPPSSGVFNPTVRENMRGILKFLAEEGSALMVSVFPHRQYK 208
                 T +  +N  L    PPS+G+F    RE +  +LK L++  S ++V ++P+  Y 
Sbjct: 163 LPISISTVVAMWN--LEQSYPPSAGMFTSQAREQLVPVLKLLSQTNSPILVKIYPYFSYA 222

Query: 209 SN-GGMSLNYAALKETKPVVEDGELKYSNLFDAMMDAFYAAIEKETIGEVTLVVGETGWP 268
           S+   + L+YA       VV+DG L YSN+FDA+ DAF  A+EKE + ++ +VV ETGWP
Sbjct: 223 SDPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGWP 282

Query: 269 TCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKPNVYLEGFIQSLFNENQKPEGESQCF 328
           + G N  I T DIA  YN  F  HI+SGKGTP++PN  ++GF+ + FNENQKP G  Q F
Sbjct: 283 SAG-NGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFLFATFNENQKPVGTEQNF 342

Query: 329 GVFDVN-MNPIYSLF 340
           G+++ N M PIY+LF
Sbjct: 343 GLYNPNDMKPIYNLF 354

[Close]

BLAST of MELO.jh003103.1 vs. ExPASy Swiss-Prot
Match: O49352 (Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN=BG4 PE=2 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 2.2e-60
Identity = 125/315 (39.68%), Postives = 193/315 (61.27%), Query Frame = 0


Query: 29  LGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRLDIPTVDVLTAFRG-SNIDLSFSVPNDML 88
           +G  YGL G+NLP P  V++L +   I RIR+  P  +VL A RG  +I+++  V +  L
Sbjct: 34  IGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTVGVKDQDL 93

Query: 89  TDMATNKALVEEWFNTYIKPFVDDFKINYIIVGDKAVPG-LDNFILPVMMSLQDLLNANY 148
             +A ++  V+ WF   I+ ++ D  I +I VG++ +PG +   +LPVM SL +L+ +  
Sbjct: 94  AALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRN 153

Query: 149 FGQVKLTTLVGYNAALVSQDPPSSGVFNPTVRENMRGILKFLAEEGSALMVSVFPHRQYK 208
              + ++T+V   + L    PPS+G+F    RE +  +LK L++  + ++V+++P+  Y 
Sbjct: 154 L-PISISTVVAM-SNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNIYPYFAYA 213

Query: 209 SN-GGMSLNYAALKETKPVVEDGELKYSNLFDAMMDAFYAAIEKETIGEVTLVVGETGWP 268
           S+   + L+YA+      VV+DG L YSN+FDA+ DAF  A+EKE +  + +VV ETGWP
Sbjct: 214 SDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPMVVSETGWP 273

Query: 269 TCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKPNVYLEGFIQSLFNENQKPEGESQCF 328
           + G N    T  IA  YN  F  HI+SGKGTP++PN  + GF+ + FNENQKP G  Q F
Sbjct: 274 SAG-NGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFNENQKPAGTEQNF 333

Query: 329 GVFD-VNMNPIYSLF 340
           G+++  +M PIY LF
Sbjct: 334 GLYNPSDMKPIYKLF 345

[Close]

BLAST of MELO.jh003103.1 vs. ExPASy Swiss-Prot
Match: O22317 (Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2)

HSP 1 Score: 219.9 bits (559), Expect = 4.3e-56
Identity = 120/314 (38.22%), Postives = 177/314 (56.37%), Query Frame = 0


Query: 29  LGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRLDIPTVDVLTAFRGSNIDLSFSVPNDMLT 88
           +G  YG+ GNNLPPP +VV L +  NI R+RL  P    L A R SNI +   VP   + 
Sbjct: 29  IGVCYGMLGNNLPPPSEVVSLYKSNNIARMRLYDPNQAALQALRNSNIQVLLDVPRSDVQ 88

Query: 89  DMATNKALVEEWFNTYIKPFVDDFKINYIIVGDKAVPGLD--NFILPVMMSLQDLL-NAN 148
            +A+N +   +W    +  +       YI VG++ +PG D   +ILP M ++ + L +A 
Sbjct: 89  SLASNPSAAGDWIRRNVVAYWPSVSFRYIAVGNELIPGSDLAQYILPAMRNIYNALSSAG 148

Query: 149 YFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVRENMRGILKFLAEEGSALMVSVFPHRQY 208
              Q+K++T V     L +  PPS+G F+   +  +  I++FLA  G+ L+V+V+P+  Y
Sbjct: 149 LQNQIKVSTAVD-TGVLGTSYPPSAGAFSSAAQAYLSPIVQFLASNGAPLLVNVYPYFSY 208

Query: 209 KSN-GGMSLNYAALKETKPVVEDGELKYSNLFDAMMDAFYAAIEKETIGEVTLVVGETGW 268
             N G +SL YA    +  VV+DG   Y NLFDA++DA +AA+E+     V +VV E+GW
Sbjct: 209 TGNPGQISLPYALFTASGVVVQDGRFSYQNLFDAIVDAVFAALERVGGANVAVVVSESGW 268

Query: 269 PTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKPNVYLEGFIQSLFNENQKPEGESQC 328
           P+ G   E  T + A+ YN     H+  G GTPR+P   +E +I  +FNENQK  G  Q 
Sbjct: 269 PSAGGGAEASTSN-ARTYNQNLIRHV--GGGTPRRPGKEIEAYIFEMFNENQKAGGIEQN 328

Query: 329 FGVFDVNMNPIYSL 339
           FG+F  N  P+Y +
Sbjct: 329 FGLFYPNKQPVYQI 338

[Close]

BLAST of MELO.jh003103.1 vs. ExPASy Swiss-Prot
Match: Q03773 (Glucan endo-1,3-beta-glucosidase OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 1.9e-51
Identity = 121/337 (35.91%), Postives = 189/337 (56.08%), Query Frame = 0


Query: 8   IVFLMASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRLDIPTVDV 67
           I FL   ++  T      D   G  YG  GNNLP P +VV L  + NIRR+R+  P+ +V
Sbjct: 16  IAFLFILLITYTGTT---DAQSGVCYGRLGNNLPTPQEVVALYNQANIRRMRIYGPSPEV 75

Query: 68  LTAFRGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVGDKAVP-- 127
           L A RGSNI+L   +PND L ++A+++    +W    IK + ++ +  Y+ VG++  P  
Sbjct: 76  LEALRGSNIELLLDIPNDNLRNLASSQDNANKWVQDNIKNYANNVRFRYVSVGNEVKPEH 135

Query: 128 GLDNFILPVMMSLQDLL-NANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVR-ENMRG 187
               F++P + ++Q  + NA    QVK++T +    AL    PPS G F    R   + G
Sbjct: 136 SFAQFLVPALENIQRAISNAGLGNQVKVSTAID-TGALAESFPPSKGSFKSDYRGAYLDG 195

Query: 188 ILKFLAEEGSALMVSVFPHRQYKSN-GGMSLNYAALKETKPVVEDGELKYSNLFDAMMDA 247
           +++FL    + LMV+V+ +  Y +N   +SL+YA  +    VV+DG L Y NLFDA +DA
Sbjct: 196 VIRFLVNNNAPLMVNVYSYFAYTANPKDISLDYALFRSPSVVVQDGSLGYRNLFDASVDA 255

Query: 248 FYAAIEKETIGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKPNV 307
            YAA+EK   G + +VV E+GWP+ G      +LD A+ YN     ++   +GTP++P  
Sbjct: 256 VYAALEKAGGGSLNIVVSESGWPSSGGT--ATSLDNARTYNTNLVRNVK--QGTPKRPGA 315

Query: 308 YLEGFIQSLFNENQKPEGESQCFGVFD-VNMNPIYSL 339
            LE ++ ++F+ENQK     + +G+F  +   P YS+
Sbjct: 316 PLETYVFAMFDENQKQPEFEKFWGLFSPITKQPKYSI 344

[Close]

BLAST of MELO.jh003103.1 vs. ExPASy Swiss-Prot
Match: P36401 (Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q' OS=Nicotiana tabacum OX=4097 PE=1 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 3.0e-49
Identity = 112/341 (32.84%), Postives = 186/341 (54.55%), Query Frame = 0


Query: 4   LVQIIVFLMASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRLDIP 63
           +  +IV L+   ++  + +       G  YG  GN LP P  VV LC + NIRR+R+  P
Sbjct: 1   MAHLIVTLLLLSVLTLATLDFTGAQAGVCYGRQGNGLPSPADVVSLCNRNNIRRMRIYDP 60

Query: 64  TVDVLTAFRGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVGDKA 123
               L A RGSNI+L   VPN  L ++A ++A  + W    ++ +  + K  YI VG++ 
Sbjct: 61  DQPTLEALRGSNIELMLGVPNPDLENVAASQANADTWVQNNVRNY-GNVKFRYIAVGNEV 120

Query: 124 VPGLDN-----FILPVMMSLQDLLNANYFG-QVKLTTLVGYNAALVSQDPPSSGVFNPTV 183
            P  +N      +L  M ++Q  ++    G Q+K++T +          PPS+G F   V
Sbjct: 121 SPLNENSKYVPVLLNAMRNIQTAISGAGLGNQIKVSTAI-ETGLTTDTSPPSNGRFKDDV 180

Query: 184 RENMRGILKFLAEEGSALMVSVFPHRQYKSNGGMSLNYAALKETKPVVEDGELKYSNLFD 243
           R+ +  I+ FL    + L+V+++P+    +N  + L YA    ++ VV D    Y NLFD
Sbjct: 181 RQFIEPIINFLVTNRAPLLVNLYPYFAIANNADIKLEYALFTSSEVVVNDNGRGYRNLFD 240

Query: 244 AMMDAFYAAIEKETIGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTP 303
           A++DA Y+A+EK +   + +VV E+GWP+ G   ++ ++D A+ YNN   SH+    G+P
Sbjct: 241 AILDATYSALEKASGSSLEIVVSESGWPSAGAG-QLTSIDNARTYNNNLISHVKG--GSP 300

Query: 304 RKPNVYLEGFIQSLFNENQKPEGESQCFGVFDVNMNPIYSL 339
           ++P+  +E ++ +LF+E+QK     + FG+F  NM P Y +
Sbjct: 301 KRPSGPIETYVFALFDEDQKDPEIEKHFGLFSANMQPKYQI 336

[Close]

BLAST of MELO.jh003103.1 vs. ExPASy TrEMBL
Match: A0A1S3BQX5 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103492533 PE=3 SV=1)

HSP 1 Score: 689 bits (1777), Expect = 2.04e-250
Identity = 340/340 (100.00%), Postives = 340/340 (100.00%), Query Frame = 0


Query: 1   MAKLVQIIVFLMASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRL 60
           MAKLVQIIVFLMASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRL
Sbjct: 1   MAKLVQIIVFLMASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRL 60

Query: 61  DIPTVDVLTAFRGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVG 120
           DIPTVDVLTAFRGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVG
Sbjct: 61  DIPTVDVLTAFRGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVG 120

Query: 121 DKAVPGLDNFILPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVREN 180
           DKAVPGLDNFILPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVREN
Sbjct: 121 DKAVPGLDNFILPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVREN 180

Query: 181 MRGILKFLAEEGSALMVSVFPHRQYKSNGGMSLNYAALKETKPVVEDGELKYSNLFDAMM 240
           MRGILKFLAEEGSALMVSVFPHRQYKSNGGMSLNYAALKETKPVVEDGELKYSNLFDAMM
Sbjct: 181 MRGILKFLAEEGSALMVSVFPHRQYKSNGGMSLNYAALKETKPVVEDGELKYSNLFDAMM 240

Query: 241 DAFYAAIEKETIGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKP 300
           DAFYAAIEKETIGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKP
Sbjct: 241 DAFYAAIEKETIGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKP 300

Query: 301 NVYLEGFIQSLFNENQKPEGESQCFGVFDVNMNPIYSLFW 340
           NVYLEGFIQSLFNENQKPEGESQCFGVFDVNMNPIYSLFW
Sbjct: 301 NVYLEGFIQSLFNENQKPEGESQCFGVFDVNMNPIYSLFW 340

[Close]

BLAST of MELO.jh003103.1 vs. ExPASy TrEMBL
Match: A0A5A7UKS6 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001680 PE=3 SV=1)

HSP 1 Score: 672 bits (1734), Expect = 4.86e-244
Identity = 329/329 (100.00%), Postives = 329/329 (100.00%), Query Frame = 0


Query: 12  MASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRLDIPTVDVLTAF 71
           MASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRLDIPTVDVLTAF
Sbjct: 1   MASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRLDIPTVDVLTAF 60

Query: 72  RGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVGDKAVPGLDNFI 131
           RGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVGDKAVPGLDNFI
Sbjct: 61  RGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVGDKAVPGLDNFI 120

Query: 132 LPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVRENMRGILKFLAEE 191
           LPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVRENMRGILKFLAEE
Sbjct: 121 LPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVRENMRGILKFLAEE 180

Query: 192 GSALMVSVFPHRQYKSNGGMSLNYAALKETKPVVEDGELKYSNLFDAMMDAFYAAIEKET 251
           GSALMVSVFPHRQYKSNGGMSLNYAALKETKPVVEDGELKYSNLFDAMMDAFYAAIEKET
Sbjct: 181 GSALMVSVFPHRQYKSNGGMSLNYAALKETKPVVEDGELKYSNLFDAMMDAFYAAIEKET 240

Query: 252 IGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKPNVYLEGFIQSL 311
           IGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKPNVYLEGFIQSL
Sbjct: 241 IGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKPNVYLEGFIQSL 300

Query: 312 FNENQKPEGESQCFGVFDVNMNPIYSLFW 340
           FNENQKPEGESQCFGVFDVNMNPIYSLFW
Sbjct: 301 FNENQKPEGESQCFGVFDVNMNPIYSLFW 329

[Close]

BLAST of MELO.jh003103.1 vs. ExPASy TrEMBL
Match: A0A0A0LZ10 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G595900 PE=3 SV=1)

HSP 1 Score: 544 bits (1401), Expect = 6.00e-193
Identity = 265/339 (78.17%), Postives = 301/339 (88.79%), Query Frame = 0


Query: 1   MAKLVQIIVFLMASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRL 60
           MAK+VQI VFLMASILM  ++V A+D+LLGAYYGL G+NLPPPWKVV LCE+YNIRR+RL
Sbjct: 1   MAKVVQI-VFLMASILMAATVVGAYDILLGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRL 60

Query: 61  DIPTVDVLTAFRGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVG 120
           D+P VDVLTAFRG  ID+SF +PN+MLTD+A NK LVEEWFNTY+K FVDDF+INYIIVG
Sbjct: 61  DVPDVDVLTAFRGGAIDVSFGIPNNMLTDLANNKGLVEEWFNTYVKTFVDDFRINYIIVG 120

Query: 121 DKAVPGLDNFILPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVREN 180
           DKA+P   NFILP+MMSLQDLLNANY GQVKLTTLVGYNAALVS+DPPSSG F+PTVRE+
Sbjct: 121 DKAIPSHANFILPIMMSLQDLLNANYLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRES 180

Query: 181 MRGILKFLAEEGSALMVSVFPHRQYKSNGGMSLNYAALKETKPVVEDGELKYSNLFDAMM 240
           MRGILKFLAEEGSALMVSVFPH++Y+ NGG+SLNYA   ET PVV+D  L+Y NLFDAM+
Sbjct: 181 MRGILKFLAEEGSALMVSVFPHQKYEFNGGISLNYAIFNETNPVVQDEGLEYYNLFDAMV 240

Query: 241 DAFYAAIEKETIGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKP 300
           DAFYAAIEKE +GEV +VVGETGWPTCGD     T  IAK+YNNKFKSHISSG GTPRKP
Sbjct: 241 DAFYAAIEKEMVGEVNIVVGETGWPTCGDISG--TQAIAKEYNNKFKSHISSGNGTPRKP 300

Query: 301 NVYLEGFIQSLFNENQKPEGESQCFGVFDVNMNPIYSLF 339
           NVYLEGFIQS+FNE++KPEG+SQC+G+FDVNM PIY LF
Sbjct: 301 NVYLEGFIQSIFNEDKKPEGDSQCYGMFDVNMKPIYPLF 336

[Close]

BLAST of MELO.jh003103.1 vs. ExPASy TrEMBL
Match: A0A6J1C4V7 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111007943 PE=3 SV=1)

HSP 1 Score: 439 bits (1129), Expect = 8.18e-152
Identity = 217/340 (63.82%), Postives = 262/340 (77.06%), Query Frame = 0


Query: 1   MAKLVQIIVFLMASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRL 60
           MAKL  IIVF MAS+    S VRA+DVLLG YYGL G+N+PPPWKVV LC KYNIRRIRL
Sbjct: 1   MAKLAHIIVFFMASM----SAVRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRL 60

Query: 61  DIPTVDVLTAFRGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVG 120
           D P ++V  AF G+ ID+SF VPNDML +M  N++ V EWFNTY++P++ DF INYIIVG
Sbjct: 61  DEPNLEVFDAFCGAGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIVG 120

Query: 121 DKAVPGLDNFILPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVREN 180
           +KA+PGLD+ ILPVM SLQ+LLNA Y GQVKLTTLVGYNAAL  +DPPSSG F+P   +N
Sbjct: 121 NKAIPGLDDCILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDN 180

Query: 181 MRGILKFLAEEGSALMVSVFPHRQYK-SNGGMSLNYAALKETKPVVEDGELKYSNLFDAM 240
           +R IL+FL  EGS LMVSV P+ +Y  SN  +SLNYA      PVV DG L Y NLFDAM
Sbjct: 181 IREILRFLLREGSPLMVSVCPYTKYAYSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAM 240

Query: 241 MDAFYAAIEKETIGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRK 300
           +D FYAA++K  +G+V + VGETGWPTCG N  I T   A  YN  FKSHISSG+GTP++
Sbjct: 241 VDPFYAAMDKLGVGDVDVAVGETGWPTCG-NGNIATPQYASAYNKNFKSHISSGRGTPKR 300

Query: 301 PNVYLEGFIQSLFNENQKPEGESQCFGVFDVNMNPIYSLF 339
           PNV + GFI+S+FNEN+ PEGESQC+G+F+V+  P+Y LF
Sbjct: 301 PNVLIRGFIKSMFNENKMPEGESQCYGIFNVDSTPLYPLF 335

[Close]

BLAST of MELO.jh003103.1 vs. ExPASy TrEMBL
Match: A0A6J1HVR6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111467895 PE=3 SV=1)

HSP 1 Score: 439 bits (1128), Expect = 1.16e-151
Identity = 214/337 (63.50%), Postives = 265/337 (78.64%), Query Frame = 0


Query: 1   MAKLVQIIVFLMASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRL 60
           M K+  +I   MA + +   +V A+DVLLGAYYGL G+NL  P  VV LC KYNI R+RL
Sbjct: 1   MVKISHMIGLFMADMFL---IVGAYDVLLGAYYGLLGDNLSSPASVVQLCRKYNISRVRL 60

Query: 61  DIPTVDVLTAFRGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVG 120
           ++  +DV+ AFRG+ IDLS  VPNDML +MATNK LVEEWFNTY++P+V+D+ INYI+VG
Sbjct: 61  EVSNIDVIEAFRGTEIDLSLGVPNDMLPNMATNKTLVEEWFNTYVEPYVNDYIINYIVVG 120

Query: 121 DKAVPGLDNFILPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVREN 180
           DKA+PGLD+ ILPVM SLQDLLNA+YFG+ KLTTLVG+NA  V+ +PPSSG FNP + EN
Sbjct: 121 DKAIPGLDDNILPVMKSLQDLLNAHYFGEAKLTTLVGFNALAVT-NPPSSGAFNPAIVEN 180

Query: 181 MRGILKFLAEEGSALMVSVFPHRQYKSNGGMSLNYAALKETKPVVEDGELKYSNLFDAMM 240
           MRGIL+FL  EGS LMVSV+ + QY   G  SL YA L E   +V DG L Y+NLFD M+
Sbjct: 181 MRGILEFLRGEGSPLMVSVYTYDQYVYGGNRSLGYATLSEQNSLVRDGMLSYNNLFDEMV 240

Query: 241 DAFYAAIEKETIGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKP 300
           DAFYAAI+KE +G+V +V+GETGWPTCG N+   T  +A +YN  FKSHISSGKGTPRK 
Sbjct: 241 DAFYAAIDKENVGDVAIVIGETGWPTCG-NYNATTPLLAAEYNRNFKSHISSGKGTPRKE 300

Query: 301 NVYLEGFIQSLFNENQKPEGESQCFGVFDVNMNPIYS 337
           N+Y+EGFI+S+FNENQKPEGES+C+G+F+V+  PIYS
Sbjct: 301 NIYIEGFIRSIFNENQKPEGESRCYGMFNVDSTPIYS 332

[Close]

BLAST of MELO.jh003103.1 vs. TAIR 10
Match: AT5G20390.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 245.7 bits (626), Expect = 5.2e-65
Identity = 133/339 (39.23%), Postives = 207/339 (61.06%), Query Frame = 0


Query: 7   IIVFLMASILMPTSLVRAHDVL--LGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRLDIPT 66
           ++ FL  + L+  S V   D    +G  YGL G+NLPPP +V++L +  ++  IR+   T
Sbjct: 9   LLKFLCVAFLLNYSNVGFVDAATNIGLNYGLLGDNLPPPSEVINLYKSLSVTNIRIFDTT 68

Query: 67  VDVLTAFRGS-NIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVGDKA 126
            DVL AFRG+ NI +   V N  L  ++ ++  V  WF T I+P++ D  I +I VG++ 
Sbjct: 69  TDVLNAFRGNRNIGVMVDVKNQDLEALSVSEEAVNTWFVTNIEPYLADVNITFIAVGNEV 128

Query: 127 VPG-LDNFILPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVRENMR 186
           +PG + +++LPVM SL +++ +     +  TT+   N  L    PPS+G F P  RE + 
Sbjct: 129 IPGEIGSYVLPVMKSLTNIVKSRSLPILISTTVAMTN--LGQSYPPSAGDFMPQAREQLT 188

Query: 187 GILKFLAEEGSALMVSVFPHRQYKSNG-GMSLNYAALKETKPVVEDGELKYSNLFDAMMD 246
            +LKFL++  + ++V+++P+  Y ++   + L+YA     K VV+DG L Y+N+FD + D
Sbjct: 189 PVLKFLSQTNTPILVNIYPYFAYAADPINIQLDYAIFNTNKVVVQDGPLGYTNMFDVIFD 248

Query: 247 AFYAAIEKETIGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKPN 306
           AF  A+EKE + ++ +VV ETGWP+ G N  + T DIA  YN  F  H+ SGKGTP++P 
Sbjct: 249 AFVWAMEKEGVKDLPMVVTETGWPSAG-NGNLTTPDIASIYNTNFVKHVESGKGTPKRPK 308

Query: 307 VYLEGFIQSLFNENQKPEGESQCFGVFD-VNMNPIYSLF 340
             + GF+ + FNENQKP G  Q FG+++  +M PIY +F
Sbjct: 309 SGISGFLFATFNENQKPAGTEQNFGLYNPTDMKPIYKMF 344

[Close]

BLAST of MELO.jh003103.1 vs. TAIR 10
Match: AT5G20340.1 (beta-1,3-glucanase 5 )

HSP 1 Score: 242.7 bits (618), Expect = 4.4e-64
Identity = 130/315 (41.27%), Postives = 195/315 (61.90%), Query Frame = 0


Query: 29  LGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRLDIPTVDVLTAFRG-SNIDLSFSVPNDML 88
           +G  YGL G+NLP P KV+ L +  +I +IR+  P  +VL A RG  +I ++  V +  L
Sbjct: 43  IGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQDL 102

Query: 89  TDMATNKALVEEWFNTYIKPFVDDFKINYIIVGDKAVPG-LDNFILPVMMSLQDLLNANY 148
             ++ ++  V+ WF T I+P++ D  I +I VG++ +PG +   +LPVM SL +L+ +  
Sbjct: 103 AALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRN 162

Query: 149 FGQVKLTTLVGYNAALVSQDPPSSGVFNPTVRENMRGILKFLAEEGSALMVSVFPHRQYK 208
                 T +  +N  L    PPS+G+F    RE +  +LK L++  S ++V ++P+  Y 
Sbjct: 163 LPISISTVVAMWN--LEQSYPPSAGMFTSQAREQLVPVLKLLSQTNSPILVKIYPYFSYA 222

Query: 209 SN-GGMSLNYAALKETKPVVEDGELKYSNLFDAMMDAFYAAIEKETIGEVTLVVGETGWP 268
           S+   + L+YA       VV+DG L YSN+FDA+ DAF  A+EKE + ++ +VV ETGWP
Sbjct: 223 SDPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGWP 282

Query: 269 TCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKPNVYLEGFIQSLFNENQKPEGESQCF 328
           + G N  I T DIA  YN  F  HI+SGKGTP++PN  ++GF+ + FNENQKP G  Q F
Sbjct: 283 SAG-NGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFLFATFNENQKPVGTEQNF 342

Query: 329 GVFDVN-MNPIYSLF 340
           G+++ N M PIY+LF
Sbjct: 343 GLYNPNDMKPIYNLF 354

[Close]

BLAST of MELO.jh003103.1 vs. TAIR 10
Match: AT5G20330.1 (beta-1,3-glucanase 4 )

HSP 1 Score: 234.2 bits (596), Expect = 1.6e-61
Identity = 125/315 (39.68%), Postives = 193/315 (61.27%), Query Frame = 0


Query: 29  LGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRLDIPTVDVLTAFRG-SNIDLSFSVPNDML 88
           +G  YGL G+NLP P  V++L +   I RIR+  P  +VL A RG  +I+++  V +  L
Sbjct: 34  IGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTVGVKDQDL 93

Query: 89  TDMATNKALVEEWFNTYIKPFVDDFKINYIIVGDKAVPG-LDNFILPVMMSLQDLLNANY 148
             +A ++  V+ WF   I+ ++ D  I +I VG++ +PG +   +LPVM SL +L+ +  
Sbjct: 94  AALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRN 153

Query: 149 FGQVKLTTLVGYNAALVSQDPPSSGVFNPTVRENMRGILKFLAEEGSALMVSVFPHRQYK 208
              + ++T+V   + L    PPS+G+F    RE +  +LK L++  + ++V+++P+  Y 
Sbjct: 154 L-PISISTVVAM-SNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNIYPYFAYA 213

Query: 209 SN-GGMSLNYAALKETKPVVEDGELKYSNLFDAMMDAFYAAIEKETIGEVTLVVGETGWP 268
           S+   + L+YA+      VV+DG L YSN+FDA+ DAF  A+EKE +  + +VV ETGWP
Sbjct: 214 SDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPMVVSETGWP 273

Query: 269 TCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKPNVYLEGFIQSLFNENQKPEGESQCF 328
           + G N    T  IA  YN  F  HI+SGKGTP++PN  + GF+ + FNENQKP G  Q F
Sbjct: 274 SAG-NGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFNENQKPAGTEQNF 333

Query: 329 GVFD-VNMNPIYSLF 340
           G+++  +M PIY LF
Sbjct: 334 GLYNPSDMKPIYKLF 345

[Close]

BLAST of MELO.jh003103.1 vs. TAIR 10
Match: AT5G20560.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 226.5 bits (576), Expect = 3.3e-59
Identity = 120/306 (39.22%), Postives = 187/306 (61.11%), Query Frame = 0


Query: 28  LLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRLDIPTVDVLTAFRGS-NIDLSFSVPNDM 87
           ++G  YGL G+NLP P  V+   +  N+ +IR+  P  DVL A RG+ +I ++  + N+ 
Sbjct: 33  VVGLNYGLLGDNLPSPSNVIKFYKSQNVAKIRIFEPNKDVLNALRGNRDIGVTVGIKNED 92

Query: 88  LTDMATNKALVEEWFNTYIKPFVDDFKINYIIVGDKAVPGLDN--FILPVMMSLQDLLNA 147
           L  +A NK  V+ WF+T I P++ D  I +I VG++A+PG  +   +LPV+ SL DL+ +
Sbjct: 93  LEALAANKDAVKSWFSTNIDPYIADVNITFITVGNQAIPGDKHGPHVLPVIQSLTDLVKS 152

Query: 148 NYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVRENMRGILKFLAEEGSALMVSVFPHRQ 207
               Q+ ++T V    +L    PPS+GV  P  R+ +  +L+ L++  + + V+++P+  
Sbjct: 153 RNL-QISISTTV-TTTSLARLKPPSAGVLTPQARQQLVPVLRLLSQTSTPIFVNIYPYYF 212

Query: 208 YKSN-GGMSLNYAALKETKPVVEDGELKYSNLFDAMMDAFYAAIEKETIGEVTLVVGETG 267
           + S+   + L YA     + VV+DG LKYSNLFDA+ DAF  A+EKE +  + LVV ETG
Sbjct: 213 HASDPKNVPLEYANFNNDQIVVKDGALKYSNLFDAIFDAFLWAMEKEGVKGLPLVVSETG 272

Query: 268 WPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKPNVYLEGFIQSLFNENQKPEGESQ 327
           WP+ G N  + T  +   Y   F  H++SGKGTP++PN  ++ +I   +NENQKP G  Q
Sbjct: 273 WPSAG-NGGMTTPALQYTYIGNFVKHVASGKGTPKRPNSRIDAYIFETYNENQKPVGIYQ 332

Query: 328 CFGVFD 330
            FG++D
Sbjct: 333 HFGLYD 335

[Close]

BLAST of MELO.jh003103.1 vs. TAIR 10
Match: AT1G33220.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 224.6 bits (571), Expect = 1.2e-58
Identity = 124/314 (39.49%), Postives = 188/314 (59.87%), Query Frame = 0


Query: 29  LGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRLDIPTVDVLTAFRG-SNIDLSFSVPNDML 88
           +G  YGL G+N P P  V++L +   I +IR+  P  +VL A RG  NI+++  V +  L
Sbjct: 34  IGLNYGLLGDNFPTPSNVLNLYKSIGITKIRIFDPKTEVLNALRGHRNIEVTVGVRDQDL 93

Query: 89  TDMATNKALVEEWFNTYIKPFVDDFKINYIIVGDKAVPG-LDNFILPVMMSLQDLLNANY 148
             ++ N+  V+ WF T I+P++ D  I +I VG++ +PG +   +LPVM SL  L+ +  
Sbjct: 94  GALSANEEAVKGWFATNIEPYLADVNIAFITVGNEVIPGPIGPQVLPVMQSLTILVKSMN 153

Query: 149 FGQVKLTTLVGYNAALVSQDPPSSGVFNPTVRENMRGILKFLAEEGSALMVSVFPHRQYK 208
              + ++T+V   + L    PPS+G F    RE +  +LK L++  + ++V+++P+  Y 
Sbjct: 154 L-PISISTVVAM-SNLEQSYPPSAGEFTSQAREQLVPVLKLLSQTSTPILVNIYPYFPYA 213

Query: 209 SN-GGMSLNYAALKETKPVVEDGELKYSNLFDAMMDAFYAAIEKETIGEVTLVVGETGWP 268
           S+   + L+YA       VV+DG L YSN+FDA+ DAF  A+EKE + ++ +VV ETGWP
Sbjct: 214 SDPTNIPLDYATFNTKATVVQDGPLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGWP 273

Query: 269 TCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKPNVYLEGFIQSLFNENQKPEGESQCF 328
           + G N  + T DIA  YN  F  HI SGKGTP++PN  ++GF+ + FNENQKP G     
Sbjct: 274 SAG-NGNLTTPDIAGTYNRNFVKHIVSGKGTPKRPNNGMDGFLFATFNENQKPAG----- 333

Query: 329 GVFDVNMNPIYSLF 340
                +M PIY LF
Sbjct: 334 ----TDMKPIYKLF 335

The following BLAST results are available for this feature:

BLAST of MELO.jh003103.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008451156.1	4.22e-250	100.00	PREDICTED: probable glucan endo-1,3-beta-glucosidase BG5 [Cucumis melo]	[more]
KAA0055784.1	1.00e-243	100.00	putative glucan endo-1,3-beta-glucosidase BG5 [Cucumis melo var. makuwa] >TYK100...	[more]
XP_004144124.2	1.24e-192	78.17	probable glucan endo-1,3-beta-glucosidase BG4 [Cucumis sativus]	[more]
KAE8653445.1	4.62e-186	78.31	hypothetical protein Csa_006879 [Cucumis sativus]	[more]
XP_038880473.1	4.82e-185	74.85	probable glucan endo-1,3-beta-glucosidase BG5 [Benincasa hispida]	[more]

BLAST of MELO.jh003103.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49353	6.2e-63	41.27	Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN...	[more]
O49352	2.2e-60	39.68	Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN...	[more]
O22317	4.3e-56	38.22	Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2	[more]
Q03773	1.9e-51	35.91	Glucan endo-1,3-beta-glucosidase OS=Glycine max OX=3847 PE=1 SV=1	[more]
P36401	3.0e-49	32.84	Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q' OS=Nicotiana tabacum OX=4...	[more]

BLAST of MELO.jh003103.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BQX5	2.04e-250	100.00	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103492533 PE=3 SV...	[more]
A0A5A7UKS6	4.86e-244	100.00	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A0A0LZ10	6.00e-193	78.17	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G595900 PE=3...	[more]
A0A6J1C4V7	8.18e-152	63.82	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111007943 ...	[more]
A0A6J1HVR6	1.16e-151	63.50	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111467895 PE=...	[more]

BLAST of MELO.jh003103.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20390.1	5.2e-65	39.23	Glycosyl hydrolase superfamily protein	[more]
AT5G20340.1	4.4e-64	41.27	beta-1,3-glucanase 5	[more]
AT5G20330.1	1.6e-61	39.68	beta-1,3-glucanase 4	[more]
AT5G20560.1	3.3e-59	39.22	Glycosyl hydrolase superfamily protein	[more]
AT1G33220.1	1.2e-58	39.49	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 29..339 e-value: 1.4E-88 score: 298.9
None	No IPR available	PANTHER	PTHR32227:SF305	GLYCOSYL HYDROLASE SUPERFAMILY PROTEIN-RELATED	coord: 18..337
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 29..338 e-value: 3.0E-62 score: 211.0
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 255..268
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 18..337
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 30..338

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO.jh003103.1.t1	MELO.jh003103.1.t1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds