MC11g_new0505 (gene) Bitter gourd (Dali-11) v1

BLAST of MC11g_new0505 vs. ExPASy Swiss-Prot
Match: Q0WP01 (Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana OX=3702 GN=NPF5.10 PE=2 SV=1)

HSP 1 Score: 694.5 bits (1791), Expect = 9.8e-199
Identity = 353/542 (65.13%), Postives = 434/542 (80.07%), Query Frame = 0

Query: 16  VDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLTDQLRQSTATAAENVN 75
           VDY  +PA +SSSGGWRSA FIIGVEV ERFAYYG+SSNLI +LT  L QSTA AA NVN
Sbjct: 19  VDYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITYLTGPLGQSTAAAAANVN 78

Query: 76  ALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSATFPSPTISASLHTEQ 135
           A SG ASLLPLLGA VADSFLGR+ TIL +SALYI+ LG+LT+SA  PS    ++L    
Sbjct: 79  AWSGTASLLPLLGAFVADSFLGRFRTILAASALYIVGLGVLTLSAMIPSDCKVSNL-LSS 138

Query: 136 ASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQETQAKSSFFNWWYFCIS 195
            SP   Q I FFS+LYL+A+AQGGH+PCVQAFGADQFD + P+E +AKSSFFNWWYF + 
Sbjct: 139 CSP-RFQVITFFSALYLVALAQGGHKPCVQAFGADQFDEKEPEECKAKSSFFNWWYFGMC 198

Query: 196 LATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYRYSNRGAEENPFVRIG 255
             T  T+ +LNY+QDNLSW LGFGIPCIAM+ AL+V LLGT TYR+S R  +++PFVRIG
Sbjct: 199 FGTLTTLWVLNYIQDNLSWALGFGIPCIAMVVALVVLLLGTCTYRFSIRREDQSPFVRIG 258

Query: 256 RVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRFLDKALVVPHGSKEDSLVCSVSEI 315
            V++ A++NW +S  ++A  +E   L++  +SQQF FL+KALV  +GS      CS+ E+
Sbjct: 259 NVYVAAVKNWSVSALDVAAAEERLGLVSCSSSQQFSFLNKALVAKNGS------CSIDEL 318

Query: 316 EEAKAVLRLGPIWVTCLAYALVFSQAFTFFTKQGVTMDRSVVPGFEVPAASLQSFIGLSV 375
           EEAK+VLRL PIW+TCL YA+VF+Q+ TFFTKQG TM+RS+ PG+++  A+LQSFI LS+
Sbjct: 319 EEAKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQGATMERSITPGYKISPATLQSFISLSI 378

Query: 376 VIFLPIYDRILVPIARTLTGEPSGITMLQRIGIGMLLSALAMVIAASVEIKRINTAQEYG 435
           VIF+PIYDR+L+PIAR+ T +P GITMLQRIG G+ LS LAMV+AA VE+KR+ TA +YG
Sbjct: 379 VIFIPIYDRVLIPIARSFTHKPGGITMLQRIGTGIFLSFLAMVVAALVEMKRLKTAADYG 438

Query: 436 LVDLPTATVPLSVWWLVPQYALIGVADGFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGV 495
           LVD P ATVP+SVWWLVPQY L G+ D F MVGLQEFFYDQVP+ LRS+GL+LYLSIFG+
Sbjct: 439 LVDSPDATVPMSVWWLVPQYVLFGITDVFAMVGLQEFFYDQVPNELRSVGLALYLSIFGI 498

Query: 496 GNFLSSFLISCIDKLTSGDGKESWFDNNLNKAHLDYFYWLLAGLSAVGLAAFLCAARTYI 555
           GNFLSSF+IS I+K TS  G+ SWF NNLN+AHLDYFYWLLA LS +GLA++L  A++Y+
Sbjct: 499 GNFLSSFMISIIEKATSQSGQASWFANNLNQAHLDYFYWLLACLSFIGLASYLYVAKSYV 552

Query: 556 YK 558
            K
Sbjct: 559 SK 552

BLAST of MC11g_new0505 vs. ExPASy Swiss-Prot
Match: Q8VZE2 (Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana OX=3702 GN=NPF5.14 PE=2 SV=2)

HSP 1 Score: 603.2 bits (1554), Expect = 3.0e-171
Identity = 307/550 (55.82%), Postives = 404/550 (73.45%), Query Frame = 0

Query: 10  ETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLTDQLRQSTAT 69
           E V  AVD+ G  A RS++G WR+A FIIGVEV ERFAYYG+ SNLI++LT  L +STA 
Sbjct: 12  EYVTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPLGESTAV 71

Query: 70  AAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSATFPSPTISA 129
           AA NVNA SG A+LLP+LGA VAD+FLGRY TI++SS +Y+L L  LT+SA     T   
Sbjct: 72  AAANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVLGLAFLTLSAFLIPNTTEV 131

Query: 130 SLHTEQASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQETQAKSSFFNW 189
           +     +S S+   +LFF SLYL+A+ Q GH+PCVQAFGADQFD +  QE   +SSFFNW
Sbjct: 132 T-----SSTSSFLNVLFFFSLYLVAIGQSGHKPCVQAFGADQFDEKDSQEKSDRSSFFNW 191

Query: 190 WYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYRYSNRGAEE- 249
           WY  +S      + ++ Y+Q+  SW  GFGIPC+ M+ +L++F+ G R YRYS R  EE 
Sbjct: 192 WYLSLSAGICFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLFVSGRRIYRYSKRRHEEE 251

Query: 250 -NPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRFLDKALVVPHGSKEDS 309
            NPF RIGRVF  AL+N  LS S++   +    L A+ + ++  F +KAL+VP+ S +  
Sbjct: 252 INPFTRIGRVFFVALKNQRLSSSDLCKVE----LEANTSPEKQSFFNKALLVPNDSSQGE 311

Query: 310 LVCSVSEIEEAKAVLRLGPIWVTCLAYALVFSQAFTFFTKQGVTMDRSVVPGFEVPAASL 369
                S++E+A A++RL P+W T LAYA+ ++Q  TFFTKQGVTMDR+++PG ++P ASL
Sbjct: 312 NASKSSDVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGVTMDRTILPGVKIPPASL 371

Query: 370 QSFIGLSVVIFLPIYDRILVPIARTLTGEPSGITMLQRIGIGMLLSALAMVIAASVEIKR 429
           Q FIG+S+V+F+PIYDR+ VPIAR +T EP GIT L+RIG G++LS + MVIAA VE KR
Sbjct: 372 QVFIGISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGIVLSTITMVIAALVEFKR 431

Query: 430 INTAQEYGLVDLPTATVPLSVWWLVPQYALIGVADGFTMVGLQEFFYDQVPSGLRSIGLS 489
           + TA+E+GL+D P AT+P+S+WWL+PQY L+G+AD +T+VG+QEFFY QVP+ LRSIGL+
Sbjct: 432 LETAKEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVPTELRSIGLA 491

Query: 490 LYLSIFGVGNFLSSFLISCIDKLTSGDGKESWFDNNLNKAHLDYFYWLLAGLSAVGLAAF 549
           LYLS  GVG+ LSS LIS ID  T GD   SWF++NLN+AHLDYFYWLLA +SAVG   F
Sbjct: 492 LYLSALGVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAIVSAVGFFTF 551

Query: 550 LCAARTYIYK 558
           L  +++YIY+
Sbjct: 552 LFISKSYIYR 552

BLAST of MC11g_new0505 vs. ExPASy Swiss-Prot
Match: Q0WSZ6 (Protein NRT1/ PTR FAMILY 5.13 OS=Arabidopsis thaliana OX=3702 GN=NPF5.13 PE=2 SV=2)

HSP 1 Score: 596.7 bits (1537), Expect = 2.8e-169
Identity = 301/551 (54.63%), Postives = 403/551 (73.14%), Query Frame = 0

Query: 10  ETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLTDQLRQSTAT 69
           E V  AVD+ G  A RS +G WR+A FIIGVEV ERFA YG+ SNLI++LT  L QSTA 
Sbjct: 12  EYVIDAVDHRGFSARRSITGRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPLGQSTAV 71

Query: 70  AAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSA-TFPSPTIS 129
           AA NVNA SG +++LPLLGA VAD+FLGRY TI+++S +Y+L L  LT+SA   P+ T  
Sbjct: 72  AAANVNAWSGISTILPLLGAFVADAFLGRYITIIIASFIYVLGLAFLTLSAFLIPNNTEV 131

Query: 130 ASLHTEQASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQETQAKSSFFN 189
            S      SPS+    LFF SLYL+A+ Q GH+PCVQAFGADQFD ++PQE   +SSFFN
Sbjct: 132 TS------SPSSFLNALFFFSLYLVAIGQSGHKPCVQAFGADQFDEKNPQENSDRSSFFN 191

Query: 190 WWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYRYSNRGAEE 249
           WWY  +     + + ++ Y+Q+N+SW LGFGIPC+ M+ +L++F+LG ++YR+S    EE
Sbjct: 192 WWYLSMCAGIGLAILVVVYIQENVSWALGFGIPCVFMVISLVLFVLGRKSYRFSKTRQEE 251

Query: 250 --NPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRFLDKALVVPHGSKED 309
             NPF RIGRVF  A +N  L+ S++   +      +  + ++  FL+KAL+VP+ S E 
Sbjct: 252 ETNPFTRIGRVFFVAFKNQRLNSSDLCKVELIEANRSQESPEELSFLNKALLVPNDSDEG 311

Query: 310 SLVCSVSEIEEAKAVLRLGPIWVTCLAYALVFSQAFTFFTKQGVTMDRSVVPGFEVPAAS 369
            + C   ++E+A A++RL P+W+T LAYA+ F+Q  TFFTKQGVTM+R++ PG E+P AS
Sbjct: 312 EVACKSRDVEDATALVRLIPVWLTTLAYAIPFAQYMTFFTKQGVTMERTIFPGVEIPPAS 371

Query: 370 LQSFIGLSVVIFLPIYDRILVPIARTLTGEPSGITMLQRIGIGMLLSALAMVIAASVEIK 429
           LQ  I +S+V+F+PIYDR+LVPI R++T +P GIT L+RIG GM+L+ L MV+AA VE K
Sbjct: 372 LQVLISISIVLFVPIYDRVLVPIGRSITKDPCGITTLKRIGTGMVLATLTMVVAALVESK 431

Query: 430 RINTAQEYGLVDLPTATVPLSVWWLVPQYALIGVADGFTMVGLQEFFYDQVPSGLRSIGL 489
           R+ TA+EYGL+D P  T+P+S+WWL PQY L+G+AD  T+VG+QEFFY QVP+ LRS+GL
Sbjct: 432 RLETAKEYGLIDQPKTTLPMSIWWLFPQYMLLGLADVHTLVGMQEFFYSQVPTELRSLGL 491

Query: 490 SLYLSIFGVGNFLSSFLISCIDKLTSGDGKESWFDNNLNKAHLDYFYWLLAGLSAVGLAA 549
           ++YLS  GVG+ LSS LI  ID  T GD   SWF++NLN+AHLDYFYWLLA +SAVG   
Sbjct: 492 AIYLSAMGVGSLLSSLLIYLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAVVSAVGFFT 551

Query: 550 FLCAARTYIYK 558
           FL  +++YIY+
Sbjct: 552 FLFISKSYIYR 556

BLAST of MC11g_new0505 vs. ExPASy Swiss-Prot
Match: Q9C7U1 (Protein NRT1/ PTR FAMILY 5.12 OS=Arabidopsis thaliana OX=3702 GN=NPF5.12 PE=2 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 5.4e-165
Identity = 300/561 (53.48%), Postives = 408/561 (72.73%), Query Frame = 0

Query: 2   ESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLTD 61
           E+  +  +E V+ +VD+ G P+ RSSSG W+S+ F +  EV E+FAY+G++SNLI + T+
Sbjct: 9   ETGAIVSNENVEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIASNLITYFTE 68

Query: 62  QLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSAT 121
            L +STA AA NVN   G A+ LPL+   +ADSFLGR+ TILL+S+ YI+ LGLLT SAT
Sbjct: 69  ALGESTAVAASNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIMGLGLLTFSAT 128

Query: 122 FPSPTISASLHTEQASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQETQ 181
            PS  +     T ++  S  + I+FF +LYL+A+ +GG + C++AFGADQFD Q P E++
Sbjct: 129 IPS--LCNDQETRESCVSQVKVIIFFCALYLIALGEGGFKVCLRAFGADQFDEQDPNESK 188

Query: 182 AKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYRY 241
           AKSS+FNW YF IS+    T  + NYVQ+NLSW LG+ IPC++M+ AL +FLLG +TYR+
Sbjct: 189 AKSSYFNWLYFAISIGILTTRLVTNYVQENLSWALGYAIPCLSMMLALFLFLLGIKTYRF 248

Query: 242 SNRG------AEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRFLDK 301
           S  G        +NPFVRIGRVF+ A RN   +PS      +T +LL + ++++FRFLD+
Sbjct: 249 STGGEGRQGKKHDNPFVRIGRVFVAAARNRRQTPS------DTCLLLPNESTKKFRFLDR 308

Query: 302 ALVVPHGSKEDSLVCSVSEIEEAKAVLRLGPIWVTCLAYALVFSQAFTFFTKQGVTMDRS 361
           A++           C   E+EEAKAVL L PIW+  L + +VF+Q+ TFFTKQG TMDRS
Sbjct: 309 AVI----------SCDSYEVEEAKAVLSLIPIWLCSLVFGIVFAQSPTFFTKQGSTMDRS 368

Query: 362 VVPGFEVPAASLQSFIGLSVVIFLPIYDRILVPIARTLTGEPSGITMLQRIGIGMLLSAL 421
           +    +VPAA+LQ FI L++++F+PIYDR+ VPIAR++T +P+GIT LQRI  G+ LS +
Sbjct: 369 ISSTLQVPAATLQCFISLAILVFIPIYDRLFVPIARSITRKPAGITTLQRISTGIFLSII 428

Query: 422 AMVIAASVEIKRINTAQEYGLVDLPTATVPLSVWWLVPQYALIGVADGFTMVGLQEFFYD 481
           +MVIAA VE+KR+ TA+++GLVD P ATVP+SV WL+PQY L GV+D FTMVGLQEFFY 
Sbjct: 429 SMVIAALVEMKRLKTARDHGLVDSPKATVPMSVCWLIPQYILFGVSDVFTMVGLQEFFYG 488

Query: 482 QVPSGLRSIGLSLYLSIFGVGNFLSSFLISCIDKLTSGDGKESWFDNNLNKAHLDYFYWL 541
           +VP  LRS+GL+LYLSI G+GNFLSSF++S I++ TS  G+ SWF NNLN+AHLDYFYWL
Sbjct: 489 EVPPQLRSMGLALYLSIIGIGNFLSSFMVSVIEEATSQSGQVSWFSNNLNQAHLDYFYWL 548

Query: 542 LAGLSAVGLAAFLCAARTYIY 557
           LA LS++     +  A++Y+Y
Sbjct: 549 LACLSSLAFIFTVYFAKSYLY 551

BLAST of MC11g_new0505 vs. ExPASy Swiss-Prot
Match: Q9SK96 (Protein NRT1/ PTR FAMILY 5.16 OS=Arabidopsis thaliana OX=3702 GN=NPF5.16 PE=2 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 9.3e-165
Identity = 301/561 (53.65%), Postives = 405/561 (72.19%), Query Frame = 0

Query: 2   ESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLTD 61
           E+ L++DS  V  +VD+ G PA +SS+GGWRSA +IIGVEV ERFAY+G+ SNLI +LT 
Sbjct: 7   EAALIEDS--VSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLITYLTG 66

Query: 62  QLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSAT 121
            L QSTATAA NVN  SG AS+LP+LGA +AD++LGRY TI+++S +YIL LGLLT+S+ 
Sbjct: 67  PLGQSTATAAVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYILGLGLLTLSSI 126

Query: 122 FPSPTISAS-LHTEQAS--PSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQ 181
                +S    H   AS  P     ILFF SLYL+A+ QGGH+PCVQAFGADQFD   P+
Sbjct: 127 LILMGLSEQRQHNRNASAKPFFWVNILFFCSLYLVAIGQGGHKPCVQAFGADQFDVGDPK 186

Query: 182 ETQAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRT 241
           E  ++ SFFNWW+  +S    +++ ++ YVQDN++W LGFGIPC+ M+ AL +FL G +T
Sbjct: 187 ERISRGSFFNWWFLSLSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALFLFGRKT 246

Query: 242 YRY--SNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRFLDKA 301
           YRY   +R  + N F RIGRVF+ A +N  L   ++ H  +  V    +   Q  FL KA
Sbjct: 247 YRYPRGDREGKNNAFARIGRVFLVAFKNRKL---KLTHSGQLEVGSYKKCKGQLEFLAKA 306

Query: 302 LVVPHGSKEDSLVCSVSEIEEAKAVLRLGPIWVTCLAYALVFSQAFTFFTKQGVTMDRSV 361
           L+   G  E    CS  ++E+A A++RL PIW+T +   + ++Q  TFFTKQGVT+DR +
Sbjct: 307 LLPGEGGVEP---CSSRDVEDAMALVRLIPIWITSVVSTIPYAQYATFFTKQGVTVDRKI 366

Query: 362 VPGFEVPAASLQSFIGLSVVIFLPIYDRILVPIARTLTGEPSGITMLQRIGIGMLLSALA 421
           +PGFE+P AS Q+ IGLS+ I +P Y+R+ +P+AR +T +PSGITMLQRIG GM+LS+L 
Sbjct: 367 LPGFEIPPASFQALIGLSIFISVPTYERVFLPLARLITKKPSGITMLQRIGAGMVLSSLN 426

Query: 422 MVIAASVEIKRINTAQEYGLVDLPTATVPLSVWWLVPQYALIGVADGFTMVGLQEFFYDQ 481
           MV+AA VE+KR+ TA+E+GLVD P AT+P+S+WW VPQY L+G+ D F++VG QEFFYDQ
Sbjct: 427 MVVAALVEMKRLETAKEHGLVDRPDATIPMSIWWFVPQYLLLGMIDVFSLVGTQEFFYDQ 486

Query: 482 VPSGLRSIGLSLYLSIFGVGNFLSSFLISCIDKLTSGDGKESWFDNNLNKAHLDYFYWLL 541
           VP+ LRSIGL+L LS  G+ +FLS FLI+ I+  T  +G +SWF+ NLN+AH+DYFYWLL
Sbjct: 487 VPTELRSIGLALSLSAMGLASFLSGFLITVINWATGKNGGDSWFNTNLNRAHVDYFYWLL 546

Query: 542 AGLSAVGLAAFLCAARTYIYK 558
           A  +A+G  AFL  +R Y+Y+
Sbjct: 547 AAFTAIGFLAFLLLSRLYVYR 559

BLAST of MC11g_new0505 vs. NCBI nr
Match: XP_022155214.1 (protein NRT1/ PTR FAMILY 5.10-like [Momordica charantia])

HSP 1 Score: 1093 bits (2826), Expect = 0.0
Identity = 561/561 (100.00%), Postives = 561/561 (100.00%), Query Frame = 0

Query: 1   MESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLT 60
           MESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLT
Sbjct: 1   MESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLT 60

Query: 61  DQLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSA 120
           DQLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSA
Sbjct: 61  DQLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSA 120

Query: 121 TFPSPTISASLHTEQASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQET 180
           TFPSPTISASLHTEQASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQET
Sbjct: 121 TFPSPTISASLHTEQASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQET 180

Query: 181 QAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYR 240
           QAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYR
Sbjct: 181 QAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYR 240

Query: 241 YSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRFLDKALVVP 300
           YSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRFLDKALVVP
Sbjct: 241 YSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRFLDKALVVP 300

Query: 301 HGSKEDSLVCSVSEIEEAKAVLRLGPIWVTCLAYALVFSQAFTFFTKQGVTMDRSVVPGF 360
           HGSKEDSLVCSVSEIEEAKAVLRLGPIWVTCLAYALVFSQAFTFFTKQGVTMDRSVVPGF
Sbjct: 301 HGSKEDSLVCSVSEIEEAKAVLRLGPIWVTCLAYALVFSQAFTFFTKQGVTMDRSVVPGF 360

Query: 361 EVPAASLQSFIGLSVVIFLPIYDRILVPIARTLTGEPSGITMLQRIGIGMLLSALAMVIA 420
           EVPAASLQSFIGLSVVIFLPIYDRILVPIARTLTGEPSGITMLQRIGIGMLLSALAMVIA
Sbjct: 361 EVPAASLQSFIGLSVVIFLPIYDRILVPIARTLTGEPSGITMLQRIGIGMLLSALAMVIA 420

Query: 421 ASVEIKRINTAQEYGLVDLPTATVPLSVWWLVPQYALIGVADGFTMVGLQEFFYDQVPSG 480
           ASVEIKRINTAQEYGLVDLPTATVPLSVWWLVPQYALIGVADGFTMVGLQEFFYDQVPSG
Sbjct: 421 ASVEIKRINTAQEYGLVDLPTATVPLSVWWLVPQYALIGVADGFTMVGLQEFFYDQVPSG 480

Query: 481 LRSIGLSLYLSIFGVGNFLSSFLISCIDKLTSGDGKESWFDNNLNKAHLDYFYWLLAGLS 540
           LRSIGLSLYLSIFGVGNFLSSFLISCIDKLTSGDGKESWFDNNLNKAHLDYFYWLLAGLS
Sbjct: 481 LRSIGLSLYLSIFGVGNFLSSFLISCIDKLTSGDGKESWFDNNLNKAHLDYFYWLLAGLS 540

Query: 541 AVGLAAFLCAARTYIYKGSTT 561
           AVGLAAFLCAARTYIYKGSTT
Sbjct: 541 AVGLAAFLCAARTYIYKGSTT 561

BLAST of MC11g_new0505 vs. NCBI nr
Match: XP_022155213.1 (protein NRT1/ PTR FAMILY 5.10-like [Momordica charantia])

HSP 1 Score: 891 bits (2302), Expect = 0.0
Identity = 459/564 (81.38%), Postives = 500/564 (88.65%), Query Frame = 0

Query: 4   PLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLTDQL 63
           PLLD  ETV+GAVDY GRP HRS+SGGWRSA+ IIGVEV ERFAYYGVSSNLINFLTDQL
Sbjct: 6   PLLD--ETVEGAVDYQGRPVHRSNSGGWRSAALIIGVEVAERFAYYGVSSNLINFLTDQL 65

Query: 64  RQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSATFP 123
            QSTA AA NVNA SGAASLLPLLGA +ADSFLGR+ TILLSSALY+L LGLLT+SATFP
Sbjct: 66  GQSTAMAAANVNAWSGAASLLPLLGAFLADSFLGRFRTILLSSALYVLGLGLLTLSATFP 125

Query: 124 SPTISA--SLHTEQA---SPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQ 183
           SP ISA    HT++A   SPS AQ ILFFSSLYL+AVAQGGH+PCVQAFGADQFD QHP+
Sbjct: 126 SPNISACQGQHTQKAVPCSPSDAQVILFFSSLYLVAVAQGGHKPCVQAFGADQFDGQHPE 185

Query: 184 ETQAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRT 243
           ET+AKSSFFNWWYFCISLATF TVNILNYVQDNLSWVLGFGIP IAM+ ALLVFLLGTR 
Sbjct: 186 ETKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWVLGFGIPFIAMVLALLVFLLGTRR 245

Query: 244 YRYSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRFLDKALV 303
           YRYSN G EENPFVRIGRVFITALRNW L+ SE+AHE+E   LL H +S+QFRFLD+ALV
Sbjct: 246 YRYSNTGDEENPFVRIGRVFITALRNWRLTSSEIAHEEEIRGLLPHHSSRQFRFLDRALV 305

Query: 304 VPHGSKEDSLVCSVSEIEEAKAVLRLGPIWVTCLAYALVFSQAFTFFTKQGVTMDRSVVP 363
           VP  SKE    CSVSE+ EAKAVLRLGPIWVTCLAYA+VFSQ+ TFFTKQGVTMDR +V 
Sbjct: 306 VPKSSKEGGRACSVSEVAEAKAVLRLGPIWVTCLAYAVVFSQSSTFFTKQGVTMDRLIVS 365

Query: 364 GFEVPAASLQSFIGLSVVIFLPIYDRILVPIARTLTGEPSGITMLQRIGIGMLLSALAMV 423
           GFE+PAASLQSFI LS+VI LPIYDR+L+PIAR  TG+PSGITMLQRIGIGMLLSAL+M+
Sbjct: 366 GFEIPAASLQSFISLSIVISLPIYDRVLIPIARNFTGKPSGITMLQRIGIGMLLSALSMI 425

Query: 424 IAASVEIKRINTAQEYGLVDLPTATVPLSVWWLVPQYALIGVADGFTMVGLQEFFYDQVP 483
           IAAS+E+KR+ TA+EYGLVDLP ATVPLS+WWLVPQY L GVAD FTMVGLQEFFYDQVP
Sbjct: 426 IAASIELKRLKTAEEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVP 485

Query: 484 SGLRSIGLSLYLSIFGVGNFLSSFLISCIDKLTSGDGKESWFDNNLNKAHLDYFYWLLAG 543
           SGLRSIGLSLYLSIFGVG+FLSSF+IS I  LTSGDGKESW DNNLNKAHLDYFYWLL+G
Sbjct: 486 SGLRSIGLSLYLSIFGVGSFLSSFIISAIVNLTSGDGKESWLDNNLNKAHLDYFYWLLSG 545

Query: 544 LSAVGLAAFLCAARTYIY-KGSTT 561
           LS +GLAAFLC ARTYIY KG+ T
Sbjct: 546 LSTIGLAAFLCFARTYIYNKGNPT 567

BLAST of MC11g_new0505 vs. NCBI nr
Match: XP_022925434.1 (protein NRT1/ PTR FAMILY 5.10-like [Cucurbita moschata])

HSP 1 Score: 887 bits (2291), Expect = 0.0
Identity = 453/565 (80.18%), Postives = 500/565 (88.50%), Query Frame = 0

Query: 1   MESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLT 60
           ME+PLL+D  TV+GAVDY GRP  RS SGGWRSA+ IIGVEV ERFAYYGVSSNLINFLT
Sbjct: 1   MEAPLLED--TVEGAVDYHGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLINFLT 60

Query: 61  DQLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSA 120
           DQL QSTATAAENVN  S AASLLPLLGA +ADSFLGRY TI LSS LYIL LGLLTVSA
Sbjct: 61  DQLGQSTATAAENVNVWSAAASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSA 120

Query: 121 TFPSPTISASLHTEQA---SPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHP 180
           T  SP+IS    TE++   SP   Q ILFFSS+Y++A AQGGH+PCVQAFGADQFDAQHP
Sbjct: 121 TLHSPSISGCQQTEKSLPCSPGLFQVILFFSSVYIVAFAQGGHKPCVQAFGADQFDAQHP 180

Query: 181 QETQAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTR 240
           +E++AKSSFFNWWYFCISLATF TVNILNYVQDNLSW LGFGIPCI+M+ AL+VFLLGTR
Sbjct: 181 EESKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTR 240

Query: 241 TYRYSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRFLDKAL 300
           TYR+ NRG EENPFVRIGRVFITA+RNW ++ SE+AHE+E   LL H +SQQFRFL+KAL
Sbjct: 241 TYRFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEEICGLLPHHSSQQFRFLNKAL 300

Query: 301 VVPHGSKEDSLVCSVSEIEEAKAVLRLGPIWVTCLAYALVFSQAFTFFTKQGVTMDRSVV 360
           +VP+ SKE    C++SE+EEAKAVLRL PIWVTCLAYA+VFSQ+ TFFTKQGVTM+RS+V
Sbjct: 301 IVPYSSKEGGHTCTISEVEEAKAVLRLVPIWVTCLAYAIVFSQSSTFFTKQGVTMNRSIV 360

Query: 361 PGFEVPAASLQSFIGLSVVIFLPIYDRILVPIARTLTGEPSGITMLQRIGIGMLLSALAM 420
           PGF+VPAASLQSFIGLS+V+ LPIYDRIL+PIAR  TG+PSGITMLQRIG GMLLSAL+M
Sbjct: 361 PGFDVPAASLQSFIGLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSM 420

Query: 421 VIAASVEIKRINTAQEYGLVDLPTATVPLSVWWLVPQYALIGVADGFTMVGLQEFFYDQV 480
           VIAA VEIKR+ TAQEYGLVDLP ATVPLS+WWLVPQY L GVAD FTMVGLQEFFYDQ 
Sbjct: 421 VIAALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQA 480

Query: 481 PSGLRSIGLSLYLSIFGVGNFLSSFLISCIDKLTSGDGKESWFDNNLNKAHLDYFYWLLA 540
           PSGLRSIGLSLYLSIFGVGNFLSSFLIS I+ L+SGDGKESWF+NNLNKAHLDYFYWLLA
Sbjct: 481 PSGLRSIGLSLYLSIFGVGNFLSSFLISAIENLSSGDGKESWFNNNLNKAHLDYFYWLLA 540

Query: 541 GLSAVGLAAFLCAARTYIY-KGSTT 561
           GLSAVGLAAFLC ARTYIY KG+TT
Sbjct: 541 GLSAVGLAAFLCFARTYIYGKGNTT 563

BLAST of MC11g_new0505 vs. NCBI nr
Match: XP_023535815.1 (protein NRT1/ PTR FAMILY 5.10-like isoform X7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 886 bits (2289), Expect = 0.0
Identity = 454/565 (80.35%), Postives = 500/565 (88.50%), Query Frame = 0

Query: 1   MESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLT 60
           ME+PLLD  ETV+GAVDY+GRP  RS  GGWRSASFIIGVEV ERFAYYGVSSNLINFLT
Sbjct: 1   MEAPLLD--ETVEGAVDYNGRPVLRSKVGGWRSASFIIGVEVAERFAYYGVSSNLINFLT 60

Query: 61  DQLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSA 120
           DQL QSTATAAENVN  SG ASLLPLLGA +ADSF+GRY TI LSS LYIL LGLLTVSA
Sbjct: 61  DQLGQSTATAAENVNVWSGVASLLPLLGAFLADSFIGRYRTIALSSVLYILGLGLLTVSA 120

Query: 121 TFPSPTISASLHTEQA---SPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHP 180
           T  SP+IS+   TE++   SP   Q ILFFSSLY++A AQGGH+PCVQAFGADQFD QHP
Sbjct: 121 TLHSPSISSCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHP 180

Query: 181 QETQAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTR 240
           +E++AKSSFFNWWYFCISLATF TVNILNYVQDNLSW LGFGIPCI+M+ AL+VFLLGTR
Sbjct: 181 EESKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTR 240

Query: 241 TYRYSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRFLDKAL 300
           TYR+ NRG EENPFVRIGRVFITA+RNW ++ SE+AHE+E   LL H +SQQFRFL+KAL
Sbjct: 241 TYRFINRGDEENPFVRIGRVFITAVRNWRINSSEIAHEEEIRGLLPHHSSQQFRFLNKAL 300

Query: 301 VVPHGSKEDSLVCSVSEIEEAKAVLRLGPIWVTCLAYALVFSQAFTFFTKQGVTMDRSVV 360
           +VP+ SKE    C++ E+EEAKAVLRL PIWVTCLAYA+VFSQ+ TFFTKQGVTM+RS+V
Sbjct: 301 IVPNSSKEGGHTCTIREVEEAKAVLRLVPIWVTCLAYAVVFSQSSTFFTKQGVTMNRSIV 360

Query: 361 PGFEVPAASLQSFIGLSVVIFLPIYDRILVPIARTLTGEPSGITMLQRIGIGMLLSALAM 420
           PGFEVPAASLQS IGLS+V+ LPIYDRIL+PIAR  TG+PSGITMLQRIG GMLLSAL+M
Sbjct: 361 PGFEVPAASLQSVIGLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSM 420

Query: 421 VIAASVEIKRINTAQEYGLVDLPTATVPLSVWWLVPQYALIGVADGFTMVGLQEFFYDQV 480
           VIAA VEIKR+ TAQEYGLVDLP ATVPLS+WWLVPQY L GVAD FTMVGLQEFFYDQ 
Sbjct: 421 VIAALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQA 480

Query: 481 PSGLRSIGLSLYLSIFGVGNFLSSFLISCIDKLTSGDGKESWFDNNLNKAHLDYFYWLLA 540
           PSGLRSIGLSLYLSIFGVGNFLSSFLIS I+KL+SGDGKESWF+NNLNKAHLDYFYWLLA
Sbjct: 481 PSGLRSIGLSLYLSIFGVGNFLSSFLISAIEKLSSGDGKESWFNNNLNKAHLDYFYWLLA 540

Query: 541 GLSAVGLAAFLCAARTYIY-KGSTT 561
           GLSAVGLAAFLC ARTYIY KG+TT
Sbjct: 541 GLSAVGLAAFLCFARTYIYGKGNTT 563

BLAST of MC11g_new0505 vs. NCBI nr
Match: XP_023535812.1 (protein NRT1/ PTR FAMILY 5.10-like isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 885 bits (2287), Expect = 0.0
Identity = 453/565 (80.18%), Postives = 502/565 (88.85%), Query Frame = 0

Query: 1   MESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLT 60
           ME+PLL+D  TV+GAVDY+GRP  RS SGGWRSA+ IIGVEV ERFAYYGVSSNLI+FLT
Sbjct: 1   MEAPLLED--TVEGAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLT 60

Query: 61  DQLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSA 120
           DQL QSTATAAENVNA SGAASLLPLLGA +ADSFLGRY TI LS+ LYIL LGLLTVSA
Sbjct: 61  DQLGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSA 120

Query: 121 TFPSPTISASLHTEQA---SPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHP 180
           T  SP+IS+   TE++   SP   Q ILFFSSLY++A AQGGH+PCVQAFGADQFD QHP
Sbjct: 121 TLHSPSISSCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHP 180

Query: 181 QETQAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTR 240
           +E++AKSSFFNWWYFCISLATF TVNILNYVQDNLSW LGFGIPCI+M+ AL+VFLLGTR
Sbjct: 181 EESKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTR 240

Query: 241 TYRYSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRFLDKAL 300
           TYR+ NRG EENPFVRIGRVFITA+RNW ++ SE+AHE+E   LL H +SQQFRFL+KAL
Sbjct: 241 TYRFINRGDEENPFVRIGRVFITAVRNWRINSSEIAHEEEIRGLLPHHSSQQFRFLNKAL 300

Query: 301 VVPHGSKEDSLVCSVSEIEEAKAVLRLGPIWVTCLAYALVFSQAFTFFTKQGVTMDRSVV 360
           +VP+ SKE    C++ E+EEAKAVLRL PIWVTCLAYA+VFSQ+ TFFTKQGVTM+RS+V
Sbjct: 301 IVPNSSKEGGHTCTIREVEEAKAVLRLVPIWVTCLAYAVVFSQSSTFFTKQGVTMNRSIV 360

Query: 361 PGFEVPAASLQSFIGLSVVIFLPIYDRILVPIARTLTGEPSGITMLQRIGIGMLLSALAM 420
           PGFEVPAASLQS IGLS+V+ LPIYDRIL+PIAR  TG+PSGITMLQRIG GMLLSAL+M
Sbjct: 361 PGFEVPAASLQSVIGLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSM 420

Query: 421 VIAASVEIKRINTAQEYGLVDLPTATVPLSVWWLVPQYALIGVADGFTMVGLQEFFYDQV 480
           VIAA VEIKR+ TAQEYGLVDLP ATVPLS+WWLVPQY L GVAD FTMVGLQEFFYDQ 
Sbjct: 421 VIAALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQA 480

Query: 481 PSGLRSIGLSLYLSIFGVGNFLSSFLISCIDKLTSGDGKESWFDNNLNKAHLDYFYWLLA 540
           PSGLRSIGLSLYLSIFGVGNFLSSFLIS I+KL+SGDGKESWF+NNLNKAHLDYFYWLLA
Sbjct: 481 PSGLRSIGLSLYLSIFGVGNFLSSFLISAIEKLSSGDGKESWFNNNLNKAHLDYFYWLLA 540

Query: 541 GLSAVGLAAFLCAARTYIY-KGSTT 561
           GLSAVGLAAFLC ARTYIY KG+TT
Sbjct: 541 GLSAVGLAAFLCFARTYIYGKGNTT 563

BLAST of MC11g_new0505 vs. ExPASy TrEMBL
Match: A0A6J1DPL2 (protein NRT1/ PTR FAMILY 5.10-like OS=Momordica charantia OX=3673 GN=LOC111022356 PE=3 SV=1)

HSP 1 Score: 1093 bits (2826), Expect = 0.0
Identity = 561/561 (100.00%), Postives = 561/561 (100.00%), Query Frame = 0

Query: 1   MESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLT 60
           MESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLT
Sbjct: 1   MESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLT 60

Query: 61  DQLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSA 120
           DQLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSA
Sbjct: 61  DQLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSA 120

Query: 121 TFPSPTISASLHTEQASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQET 180
           TFPSPTISASLHTEQASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQET
Sbjct: 121 TFPSPTISASLHTEQASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQET 180

Query: 181 QAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYR 240
           QAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYR
Sbjct: 181 QAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYR 240

Query: 241 YSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRFLDKALVVP 300
           YSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRFLDKALVVP
Sbjct: 241 YSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRFLDKALVVP 300

Query: 301 HGSKEDSLVCSVSEIEEAKAVLRLGPIWVTCLAYALVFSQAFTFFTKQGVTMDRSVVPGF 360
           HGSKEDSLVCSVSEIEEAKAVLRLGPIWVTCLAYALVFSQAFTFFTKQGVTMDRSVVPGF
Sbjct: 301 HGSKEDSLVCSVSEIEEAKAVLRLGPIWVTCLAYALVFSQAFTFFTKQGVTMDRSVVPGF 360

Query: 361 EVPAASLQSFIGLSVVIFLPIYDRILVPIARTLTGEPSGITMLQRIGIGMLLSALAMVIA 420
           EVPAASLQSFIGLSVVIFLPIYDRILVPIARTLTGEPSGITMLQRIGIGMLLSALAMVIA
Sbjct: 361 EVPAASLQSFIGLSVVIFLPIYDRILVPIARTLTGEPSGITMLQRIGIGMLLSALAMVIA 420

Query: 421 ASVEIKRINTAQEYGLVDLPTATVPLSVWWLVPQYALIGVADGFTMVGLQEFFYDQVPSG 480
           ASVEIKRINTAQEYGLVDLPTATVPLSVWWLVPQYALIGVADGFTMVGLQEFFYDQVPSG
Sbjct: 421 ASVEIKRINTAQEYGLVDLPTATVPLSVWWLVPQYALIGVADGFTMVGLQEFFYDQVPSG 480

Query: 481 LRSIGLSLYLSIFGVGNFLSSFLISCIDKLTSGDGKESWFDNNLNKAHLDYFYWLLAGLS 540
           LRSIGLSLYLSIFGVGNFLSSFLISCIDKLTSGDGKESWFDNNLNKAHLDYFYWLLAGLS
Sbjct: 481 LRSIGLSLYLSIFGVGNFLSSFLISCIDKLTSGDGKESWFDNNLNKAHLDYFYWLLAGLS 540

Query: 541 AVGLAAFLCAARTYIYKGSTT 561
           AVGLAAFLCAARTYIYKGSTT
Sbjct: 541 AVGLAAFLCAARTYIYKGSTT 561

BLAST of MC11g_new0505 vs. ExPASy TrEMBL
Match: A0A6J1DMD5 (protein NRT1/ PTR FAMILY 5.10-like OS=Momordica charantia OX=3673 GN=LOC111022355 PE=3 SV=1)

HSP 1 Score: 891 bits (2302), Expect = 0.0
Identity = 459/564 (81.38%), Postives = 500/564 (88.65%), Query Frame = 0

Query: 4   PLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLTDQL 63
           PLLD  ETV+GAVDY GRP HRS+SGGWRSA+ IIGVEV ERFAYYGVSSNLINFLTDQL
Sbjct: 6   PLLD--ETVEGAVDYQGRPVHRSNSGGWRSAALIIGVEVAERFAYYGVSSNLINFLTDQL 65

Query: 64  RQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSATFP 123
            QSTA AA NVNA SGAASLLPLLGA +ADSFLGR+ TILLSSALY+L LGLLT+SATFP
Sbjct: 66  GQSTAMAAANVNAWSGAASLLPLLGAFLADSFLGRFRTILLSSALYVLGLGLLTLSATFP 125

Query: 124 SPTISA--SLHTEQA---SPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQ 183
           SP ISA    HT++A   SPS AQ ILFFSSLYL+AVAQGGH+PCVQAFGADQFD QHP+
Sbjct: 126 SPNISACQGQHTQKAVPCSPSDAQVILFFSSLYLVAVAQGGHKPCVQAFGADQFDGQHPE 185

Query: 184 ETQAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRT 243
           ET+AKSSFFNWWYFCISLATF TVNILNYVQDNLSWVLGFGIP IAM+ ALLVFLLGTR 
Sbjct: 186 ETKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWVLGFGIPFIAMVLALLVFLLGTRR 245

Query: 244 YRYSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRFLDKALV 303
           YRYSN G EENPFVRIGRVFITALRNW L+ SE+AHE+E   LL H +S+QFRFLD+ALV
Sbjct: 246 YRYSNTGDEENPFVRIGRVFITALRNWRLTSSEIAHEEEIRGLLPHHSSRQFRFLDRALV 305

Query: 304 VPHGSKEDSLVCSVSEIEEAKAVLRLGPIWVTCLAYALVFSQAFTFFTKQGVTMDRSVVP 363
           VP  SKE    CSVSE+ EAKAVLRLGPIWVTCLAYA+VFSQ+ TFFTKQGVTMDR +V 
Sbjct: 306 VPKSSKEGGRACSVSEVAEAKAVLRLGPIWVTCLAYAVVFSQSSTFFTKQGVTMDRLIVS 365

Query: 364 GFEVPAASLQSFIGLSVVIFLPIYDRILVPIARTLTGEPSGITMLQRIGIGMLLSALAMV 423
           GFE+PAASLQSFI LS+VI LPIYDR+L+PIAR  TG+PSGITMLQRIGIGMLLSAL+M+
Sbjct: 366 GFEIPAASLQSFISLSIVISLPIYDRVLIPIARNFTGKPSGITMLQRIGIGMLLSALSMI 425

Query: 424 IAASVEIKRINTAQEYGLVDLPTATVPLSVWWLVPQYALIGVADGFTMVGLQEFFYDQVP 483
           IAAS+E+KR+ TA+EYGLVDLP ATVPLS+WWLVPQY L GVAD FTMVGLQEFFYDQVP
Sbjct: 426 IAASIELKRLKTAEEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVP 485

Query: 484 SGLRSIGLSLYLSIFGVGNFLSSFLISCIDKLTSGDGKESWFDNNLNKAHLDYFYWLLAG 543
           SGLRSIGLSLYLSIFGVG+FLSSF+IS I  LTSGDGKESW DNNLNKAHLDYFYWLL+G
Sbjct: 486 SGLRSIGLSLYLSIFGVGSFLSSFIISAIVNLTSGDGKESWLDNNLNKAHLDYFYWLLSG 545

Query: 544 LSAVGLAAFLCAARTYIY-KGSTT 561
           LS +GLAAFLC ARTYIY KG+ T
Sbjct: 546 LSTIGLAAFLCFARTYIYNKGNPT 567

BLAST of MC11g_new0505 vs. ExPASy TrEMBL
Match: A0A6J1EC56 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111432731 PE=3 SV=1)

HSP 1 Score: 887 bits (2291), Expect = 0.0
Identity = 453/565 (80.18%), Postives = 500/565 (88.50%), Query Frame = 0

Query: 1   MESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLT 60
           ME+PLL+D  TV+GAVDY GRP  RS SGGWRSA+ IIGVEV ERFAYYGVSSNLINFLT
Sbjct: 1   MEAPLLED--TVEGAVDYHGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLINFLT 60

Query: 61  DQLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSA 120
           DQL QSTATAAENVN  S AASLLPLLGA +ADSFLGRY TI LSS LYIL LGLLTVSA
Sbjct: 61  DQLGQSTATAAENVNVWSAAASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSA 120

Query: 121 TFPSPTISASLHTEQA---SPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHP 180
           T  SP+IS    TE++   SP   Q ILFFSS+Y++A AQGGH+PCVQAFGADQFDAQHP
Sbjct: 121 TLHSPSISGCQQTEKSLPCSPGLFQVILFFSSVYIVAFAQGGHKPCVQAFGADQFDAQHP 180

Query: 181 QETQAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTR 240
           +E++AKSSFFNWWYFCISLATF TVNILNYVQDNLSW LGFGIPCI+M+ AL+VFLLGTR
Sbjct: 181 EESKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTR 240

Query: 241 TYRYSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRFLDKAL 300
           TYR+ NRG EENPFVRIGRVFITA+RNW ++ SE+AHE+E   LL H +SQQFRFL+KAL
Sbjct: 241 TYRFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEEICGLLPHHSSQQFRFLNKAL 300

Query: 301 VVPHGSKEDSLVCSVSEIEEAKAVLRLGPIWVTCLAYALVFSQAFTFFTKQGVTMDRSVV 360
           +VP+ SKE    C++SE+EEAKAVLRL PIWVTCLAYA+VFSQ+ TFFTKQGVTM+RS+V
Sbjct: 301 IVPYSSKEGGHTCTISEVEEAKAVLRLVPIWVTCLAYAIVFSQSSTFFTKQGVTMNRSIV 360

Query: 361 PGFEVPAASLQSFIGLSVVIFLPIYDRILVPIARTLTGEPSGITMLQRIGIGMLLSALAM 420
           PGF+VPAASLQSFIGLS+V+ LPIYDRIL+PIAR  TG+PSGITMLQRIG GMLLSAL+M
Sbjct: 361 PGFDVPAASLQSFIGLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSM 420

Query: 421 VIAASVEIKRINTAQEYGLVDLPTATVPLSVWWLVPQYALIGVADGFTMVGLQEFFYDQV 480
           VIAA VEIKR+ TAQEYGLVDLP ATVPLS+WWLVPQY L GVAD FTMVGLQEFFYDQ 
Sbjct: 421 VIAALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQA 480

Query: 481 PSGLRSIGLSLYLSIFGVGNFLSSFLISCIDKLTSGDGKESWFDNNLNKAHLDYFYWLLA 540
           PSGLRSIGLSLYLSIFGVGNFLSSFLIS I+ L+SGDGKESWF+NNLNKAHLDYFYWLLA
Sbjct: 481 PSGLRSIGLSLYLSIFGVGNFLSSFLISAIENLSSGDGKESWFNNNLNKAHLDYFYWLLA 540

Query: 541 GLSAVGLAAFLCAARTYIY-KGSTT 561
           GLSAVGLAAFLC ARTYIY KG+TT
Sbjct: 541 GLSAVGLAAFLCFARTYIYGKGNTT 563

BLAST of MC11g_new0505 vs. ExPASy TrEMBL
Match: A0A6J1IG54 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita maxima OX=3661 GN=LOC111472583 PE=3 SV=1)

HSP 1 Score: 881 bits (2277), Expect = 0.0
Identity = 451/565 (79.82%), Postives = 500/565 (88.50%), Query Frame = 0

Query: 1   MESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLT 60
           ME+PLL+D  TV+GAVDY+GRP  RS SGGWRSA+ IIGVEV ERFAYYGVSSNLI+FLT
Sbjct: 1   MEAPLLED--TVEGAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLT 60

Query: 61  DQLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSA 120
           DQL QSTATAAENVNA SGAASLLPLLGA +ADSFLGRY TI LS+ LYIL LGLLTVSA
Sbjct: 61  DQLGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSA 120

Query: 121 TFPSPTISASLHTEQA---SPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHP 180
           T  SP IS    TE++   SP   Q ILFFSSLY++A AQGGH+PCVQAFGADQFD QHP
Sbjct: 121 TLHSPRISGCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHP 180

Query: 181 QETQAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTR 240
           +E++AKSSFFNWWYFCISLATF TVNILNYVQDNLSW LGFGIPCI+M+ AL+VFLLGTR
Sbjct: 181 EESKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTR 240

Query: 241 TYRYSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRFLDKAL 300
           TYR+ NRG EENPFVRIGRVFITA+RNW ++ SE+AHE+E   LL H +SQQFRFL+KAL
Sbjct: 241 TYRFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEEIRGLLPHHSSQQFRFLNKAL 300

Query: 301 VVPHGSKEDSLVCSVSEIEEAKAVLRLGPIWVTCLAYALVFSQAFTFFTKQGVTMDRSVV 360
           +VP+ SKE    CS+SE+EEAKAVLRL PIWVTCLAYA+VFSQ+ TFFTKQGVTM+RS+V
Sbjct: 301 IVPNSSKEGGHTCSISEVEEAKAVLRLVPIWVTCLAYAIVFSQSSTFFTKQGVTMNRSIV 360

Query: 361 PGFEVPAASLQSFIGLSVVIFLPIYDRILVPIARTLTGEPSGITMLQRIGIGMLLSALAM 420
           PGF+VPAASLQSFI LS+V+ L IYDRIL+PIAR  TG+PSGITMLQRIG GMLLSAL+M
Sbjct: 361 PGFDVPAASLQSFISLSIVVSLLIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSM 420

Query: 421 VIAASVEIKRINTAQEYGLVDLPTATVPLSVWWLVPQYALIGVADGFTMVGLQEFFYDQV 480
           VIAA VEIKR+ TAQEYGLVDLP ATVPLS+WWLVPQY L GVAD FTMVGLQEFFYDQ 
Sbjct: 421 VIAALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQA 480

Query: 481 PSGLRSIGLSLYLSIFGVGNFLSSFLISCIDKLTSGDGKESWFDNNLNKAHLDYFYWLLA 540
           PSGLRSIGLSLYLSIFGVGNFLSSFLIS I+KL+SGDGKESWF++NLNKAHLDYFYWLLA
Sbjct: 481 PSGLRSIGLSLYLSIFGVGNFLSSFLISAIEKLSSGDGKESWFNSNLNKAHLDYFYWLLA 540

Query: 541 GLSAVGLAAFLCAARTYIY-KGSTT 561
           GLSA+GLAAFLC ARTYIY KG+TT
Sbjct: 541 GLSAIGLAAFLCFARTYIYSKGNTT 563

BLAST of MC11g_new0505 vs. ExPASy TrEMBL
Match: A0A6J1EHW2 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111432710 PE=3 SV=1)

HSP 1 Score: 877 bits (2266), Expect = 0.0
Identity = 447/564 (79.26%), Postives = 496/564 (87.94%), Query Frame = 0

Query: 1   MESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLT 60
           ME+PLL+D  TV+ AVDY+GRP  RS SGGWRSA+ IIGVEV ERFAYYGVSSNLI+FLT
Sbjct: 1   MEAPLLED--TVECAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLT 60

Query: 61  DQLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSA 120
           DQL QSTATAAENVNA SGAASLLPLLGA +ADSFLGRY TI LS+ LYIL LGLLTVSA
Sbjct: 61  DQLGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSA 120

Query: 121 TFPSPTISASLHTEQA---SPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHP 180
           T  SP+IS    TE++   SP   Q ILFF SLY++A AQGGH+PCVQAFGADQFDAQHP
Sbjct: 121 TLHSPSISGCQQTEKSLPCSPGLFQVILFFFSLYVVAFAQGGHKPCVQAFGADQFDAQHP 180

Query: 181 QETQAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTR 240
           +E++AKSSFFNWWYFCISLATF TVNILNYVQDNLSW LGFGIPCI+M+ AL+VFLLGTR
Sbjct: 181 EESKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTR 240

Query: 241 TYRYSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRFLDKAL 300
           TYR+ NRG EENPFVRIGRVFITA+RNW ++ SE+AHE+E   LL H +SQQFRFL+KAL
Sbjct: 241 TYRFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEEIRGLLPHHSSQQFRFLNKAL 300

Query: 301 VVPHGSKEDSLVCSVSEIEEAKAVLRLGPIWVTCLAYALVFSQAFTFFTKQGVTMDRSVV 360
           +VP+ SKE    C++SE+EEAKAVLRL PIWVTCLAYA+VFSQ+ TFFTKQGVTM+RS+V
Sbjct: 301 LVPNSSKEGGHTCTISEVEEAKAVLRLVPIWVTCLAYAIVFSQSSTFFTKQGVTMNRSIV 360

Query: 361 PGFEVPAASLQSFIGLSVVIFLPIYDRILVPIARTLTGEPSGITMLQRIGIGMLLSALAM 420
           PGF+VPAASLQSFI LS+V+ LPIYDRIL+PIAR  TG+PSGITMLQRIG GMLLSAL+M
Sbjct: 361 PGFDVPAASLQSFISLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSM 420

Query: 421 VIAASVEIKRINTAQEYGLVDLPTATVPLSVWWLVPQYALIGVADGFTMVGLQEFFYDQV 480
           VIAA VEIKR+ TAQEYGLVDLP ATVPLS+WWLVPQY L GVAD FTMVGLQEFFYDQ 
Sbjct: 421 VIAALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQA 480

Query: 481 PSGLRSIGLSLYLSIFGVGNFLSSFLISCIDKLTSGDGKESWFDNNLNKAHLDYFYWLLA 540
           PSGLRSIGLSLYLSIFGVGNFLSSFLIS I+ L+SGDGKESWF+NNLNKAHLDYFYWLLA
Sbjct: 481 PSGLRSIGLSLYLSIFGVGNFLSSFLISAIENLSSGDGKESWFNNNLNKAHLDYFYWLLA 540

Query: 541 GLSAVGLAAFLCAARTYIYKGSTT 561
           GLSA+GLAAFLC ARTYIY    T
Sbjct: 541 GLSAIGLAAFLCFARTYIYSKKNT 562

BLAST of MC11g_new0505 vs. TAIR 10
Match: AT1G22540.1 (Major facilitator superfamily protein )

HSP 1 Score: 694.5 bits (1791), Expect = 7.0e-200
Identity = 353/542 (65.13%), Postives = 434/542 (80.07%), Query Frame = 0

Query: 16  VDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLTDQLRQSTATAAENVN 75
           VDY  +PA +SSSGGWRSA FIIGVEV ERFAYYG+SSNLI +LT  L QSTA AA NVN
Sbjct: 19  VDYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITYLTGPLGQSTAAAAANVN 78

Query: 76  ALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSATFPSPTISASLHTEQ 135
           A SG ASLLPLLGA VADSFLGR+ TIL +SALYI+ LG+LT+SA  PS    ++L    
Sbjct: 79  AWSGTASLLPLLGAFVADSFLGRFRTILAASALYIVGLGVLTLSAMIPSDCKVSNL-LSS 138

Query: 136 ASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQETQAKSSFFNWWYFCIS 195
            SP   Q I FFS+LYL+A+AQGGH+PCVQAFGADQFD + P+E +AKSSFFNWWYF + 
Sbjct: 139 CSP-RFQVITFFSALYLVALAQGGHKPCVQAFGADQFDEKEPEECKAKSSFFNWWYFGMC 198

Query: 196 LATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYRYSNRGAEENPFVRIG 255
             T  T+ +LNY+QDNLSW LGFGIPCIAM+ AL+V LLGT TYR+S R  +++PFVRIG
Sbjct: 199 FGTLTTLWVLNYIQDNLSWALGFGIPCIAMVVALVVLLLGTCTYRFSIRREDQSPFVRIG 258

Query: 256 RVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRFLDKALVVPHGSKEDSLVCSVSEI 315
            V++ A++NW +S  ++A  +E   L++  +SQQF FL+KALV  +GS      CS+ E+
Sbjct: 259 NVYVAAVKNWSVSALDVAAAEERLGLVSCSSSQQFSFLNKALVAKNGS------CSIDEL 318

Query: 316 EEAKAVLRLGPIWVTCLAYALVFSQAFTFFTKQGVTMDRSVVPGFEVPAASLQSFIGLSV 375
           EEAK+VLRL PIW+TCL YA+VF+Q+ TFFTKQG TM+RS+ PG+++  A+LQSFI LS+
Sbjct: 319 EEAKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQGATMERSITPGYKISPATLQSFISLSI 378

Query: 376 VIFLPIYDRILVPIARTLTGEPSGITMLQRIGIGMLLSALAMVIAASVEIKRINTAQEYG 435
           VIF+PIYDR+L+PIAR+ T +P GITMLQRIG G+ LS LAMV+AA VE+KR+ TA +YG
Sbjct: 379 VIFIPIYDRVLIPIARSFTHKPGGITMLQRIGTGIFLSFLAMVVAALVEMKRLKTAADYG 438

Query: 436 LVDLPTATVPLSVWWLVPQYALIGVADGFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGV 495
           LVD P ATVP+SVWWLVPQY L G+ D F MVGLQEFFYDQVP+ LRS+GL+LYLSIFG+
Sbjct: 439 LVDSPDATVPMSVWWLVPQYVLFGITDVFAMVGLQEFFYDQVPNELRSVGLALYLSIFGI 498

Query: 496 GNFLSSFLISCIDKLTSGDGKESWFDNNLNKAHLDYFYWLLAGLSAVGLAAFLCAARTYI 555
           GNFLSSF+IS I+K TS  G+ SWF NNLN+AHLDYFYWLLA LS +GLA++L  A++Y+
Sbjct: 499 GNFLSSFMISIIEKATSQSGQASWFANNLNQAHLDYFYWLLACLSFIGLASYLYVAKSYV 552

Query: 556 YK 558
            K
Sbjct: 559 SK 552

BLAST of MC11g_new0505 vs. TAIR 10
Match: AT1G72120.1 (Major facilitator superfamily protein )

HSP 1 Score: 603.2 bits (1554), Expect = 2.1e-172
Identity = 307/550 (55.82%), Postives = 404/550 (73.45%), Query Frame = 0

Query: 10  ETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLTDQLRQSTAT 69
           E V  AVD+ G  A RS++G WR+A FIIGVEV ERFAYYG+ SNLI++LT  L +STA 
Sbjct: 12  EYVTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPLGESTAV 71

Query: 70  AAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSATFPSPTISA 129
           AA NVNA SG A+LLP+LGA VAD+FLGRY TI++SS +Y+L L  LT+SA     T   
Sbjct: 72  AAANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVLGLAFLTLSAFLIPNTTEV 131

Query: 130 SLHTEQASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQETQAKSSFFNW 189
           +     +S S+   +LFF SLYL+A+ Q GH+PCVQAFGADQFD +  QE   +SSFFNW
Sbjct: 132 T-----SSTSSFLNVLFFFSLYLVAIGQSGHKPCVQAFGADQFDEKDSQEKSDRSSFFNW 191

Query: 190 WYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYRYSNRGAEE- 249
           WY  +S      + ++ Y+Q+  SW  GFGIPC+ M+ +L++F+ G R YRYS R  EE 
Sbjct: 192 WYLSLSAGICFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLFVSGRRIYRYSKRRHEEE 251

Query: 250 -NPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRFLDKALVVPHGSKEDS 309
            NPF RIGRVF  AL+N  LS S++   +    L A+ + ++  F +KAL+VP+ S +  
Sbjct: 252 INPFTRIGRVFFVALKNQRLSSSDLCKVE----LEANTSPEKQSFFNKALLVPNDSSQGE 311

Query: 310 LVCSVSEIEEAKAVLRLGPIWVTCLAYALVFSQAFTFFTKQGVTMDRSVVPGFEVPAASL 369
                S++E+A A++RL P+W T LAYA+ ++Q  TFFTKQGVTMDR+++PG ++P ASL
Sbjct: 312 NASKSSDVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGVTMDRTILPGVKIPPASL 371

Query: 370 QSFIGLSVVIFLPIYDRILVPIARTLTGEPSGITMLQRIGIGMLLSALAMVIAASVEIKR 429
           Q FIG+S+V+F+PIYDR+ VPIAR +T EP GIT L+RIG G++LS + MVIAA VE KR
Sbjct: 372 QVFIGISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGIVLSTITMVIAALVEFKR 431

Query: 430 INTAQEYGLVDLPTATVPLSVWWLVPQYALIGVADGFTMVGLQEFFYDQVPSGLRSIGLS 489
           + TA+E+GL+D P AT+P+S+WWL+PQY L+G+AD +T+VG+QEFFY QVP+ LRSIGL+
Sbjct: 432 LETAKEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVPTELRSIGLA 491

Query: 490 LYLSIFGVGNFLSSFLISCIDKLTSGDGKESWFDNNLNKAHLDYFYWLLAGLSAVGLAAF 549
           LYLS  GVG+ LSS LIS ID  T GD   SWF++NLN+AHLDYFYWLLA +SAVG   F
Sbjct: 492 LYLSALGVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAIVSAVGFFTF 551

Query: 550 LCAARTYIYK 558
           L  +++YIY+
Sbjct: 552 LFISKSYIYR 552

BLAST of MC11g_new0505 vs. TAIR 10
Match: AT1G72125.1 (Major facilitator superfamily protein )

HSP 1 Score: 596.7 bits (1537), Expect = 2.0e-170
Identity = 301/551 (54.63%), Postives = 403/551 (73.14%), Query Frame = 0

Query: 10  ETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLTDQLRQSTAT 69
           E V  AVD+ G  A RS +G WR+A FIIGVEV ERFA YG+ SNLI++LT  L QSTA 
Sbjct: 12  EYVIDAVDHRGFSARRSITGRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPLGQSTAV 71

Query: 70  AAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSA-TFPSPTIS 129
           AA NVNA SG +++LPLLGA VAD+FLGRY TI+++S +Y+L L  LT+SA   P+ T  
Sbjct: 72  AAANVNAWSGISTILPLLGAFVADAFLGRYITIIIASFIYVLGLAFLTLSAFLIPNNTEV 131

Query: 130 ASLHTEQASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQETQAKSSFFN 189
            S      SPS+    LFF SLYL+A+ Q GH+PCVQAFGADQFD ++PQE   +SSFFN
Sbjct: 132 TS------SPSSFLNALFFFSLYLVAIGQSGHKPCVQAFGADQFDEKNPQENSDRSSFFN 191

Query: 190 WWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYRYSNRGAEE 249
           WWY  +     + + ++ Y+Q+N+SW LGFGIPC+ M+ +L++F+LG ++YR+S    EE
Sbjct: 192 WWYLSMCAGIGLAILVVVYIQENVSWALGFGIPCVFMVISLVLFVLGRKSYRFSKTRQEE 251

Query: 250 --NPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRFLDKALVVPHGSKED 309
             NPF RIGRVF  A +N  L+ S++   +      +  + ++  FL+KAL+VP+ S E 
Sbjct: 252 ETNPFTRIGRVFFVAFKNQRLNSSDLCKVELIEANRSQESPEELSFLNKALLVPNDSDEG 311

Query: 310 SLVCSVSEIEEAKAVLRLGPIWVTCLAYALVFSQAFTFFTKQGVTMDRSVVPGFEVPAAS 369
            + C   ++E+A A++RL P+W+T LAYA+ F+Q  TFFTKQGVTM+R++ PG E+P AS
Sbjct: 312 EVACKSRDVEDATALVRLIPVWLTTLAYAIPFAQYMTFFTKQGVTMERTIFPGVEIPPAS 371

Query: 370 LQSFIGLSVVIFLPIYDRILVPIARTLTGEPSGITMLQRIGIGMLLSALAMVIAASVEIK 429
           LQ  I +S+V+F+PIYDR+LVPI R++T +P GIT L+RIG GM+L+ L MV+AA VE K
Sbjct: 372 LQVLISISIVLFVPIYDRVLVPIGRSITKDPCGITTLKRIGTGMVLATLTMVVAALVESK 431

Query: 430 RINTAQEYGLVDLPTATVPLSVWWLVPQYALIGVADGFTMVGLQEFFYDQVPSGLRSIGL 489
           R+ TA+EYGL+D P  T+P+S+WWL PQY L+G+AD  T+VG+QEFFY QVP+ LRS+GL
Sbjct: 432 RLETAKEYGLIDQPKTTLPMSIWWLFPQYMLLGLADVHTLVGMQEFFYSQVPTELRSLGL 491

Query: 490 SLYLSIFGVGNFLSSFLISCIDKLTSGDGKESWFDNNLNKAHLDYFYWLLAGLSAVGLAA 549
           ++YLS  GVG+ LSS LI  ID  T GD   SWF++NLN+AHLDYFYWLLA +SAVG   
Sbjct: 492 AIYLSAMGVGSLLSSLLIYLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAVVSAVGFFT 551

Query: 550 FLCAARTYIYK 558
           FL  +++YIY+
Sbjct: 552 FLFISKSYIYR 556

BLAST of MC11g_new0505 vs. TAIR 10
Match: AT1G72140.1 (Major facilitator superfamily protein )

HSP 1 Score: 582.4 bits (1500), Expect = 3.9e-166
Identity = 300/561 (53.48%), Postives = 408/561 (72.73%), Query Frame = 0

Query: 2   ESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLTD 61
           E+  +  +E V+ +VD+ G P+ RSSSG W+S+ F +  EV E+FAY+G++SNLI + T+
Sbjct: 9   ETGAIVSNENVEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIASNLITYFTE 68

Query: 62  QLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSAT 121
            L +STA AA NVN   G A+ LPL+   +ADSFLGR+ TILL+S+ YI+ LGLLT SAT
Sbjct: 69  ALGESTAVAASNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIMGLGLLTFSAT 128

Query: 122 FPSPTISASLHTEQASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQETQ 181
            PS  +     T ++  S  + I+FF +LYL+A+ +GG + C++AFGADQFD Q P E++
Sbjct: 129 IPS--LCNDQETRESCVSQVKVIIFFCALYLIALGEGGFKVCLRAFGADQFDEQDPNESK 188

Query: 182 AKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYRY 241
           AKSS+FNW YF IS+    T  + NYVQ+NLSW LG+ IPC++M+ AL +FLLG +TYR+
Sbjct: 189 AKSSYFNWLYFAISIGILTTRLVTNYVQENLSWALGYAIPCLSMMLALFLFLLGIKTYRF 248

Query: 242 SNRG------AEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRFLDK 301
           S  G        +NPFVRIGRVF+ A RN   +PS      +T +LL + ++++FRFLD+
Sbjct: 249 STGGEGRQGKKHDNPFVRIGRVFVAAARNRRQTPS------DTCLLLPNESTKKFRFLDR 308

Query: 302 ALVVPHGSKEDSLVCSVSEIEEAKAVLRLGPIWVTCLAYALVFSQAFTFFTKQGVTMDRS 361
           A++           C   E+EEAKAVL L PIW+  L + +VF+Q+ TFFTKQG TMDRS
Sbjct: 309 AVI----------SCDSYEVEEAKAVLSLIPIWLCSLVFGIVFAQSPTFFTKQGSTMDRS 368

Query: 362 VVPGFEVPAASLQSFIGLSVVIFLPIYDRILVPIARTLTGEPSGITMLQRIGIGMLLSAL 421
           +    +VPAA+LQ FI L++++F+PIYDR+ VPIAR++T +P+GIT LQRI  G+ LS +
Sbjct: 369 ISSTLQVPAATLQCFISLAILVFIPIYDRLFVPIARSITRKPAGITTLQRISTGIFLSII 428

Query: 422 AMVIAASVEIKRINTAQEYGLVDLPTATVPLSVWWLVPQYALIGVADGFTMVGLQEFFYD 481
           +MVIAA VE+KR+ TA+++GLVD P ATVP+SV WL+PQY L GV+D FTMVGLQEFFY 
Sbjct: 429 SMVIAALVEMKRLKTARDHGLVDSPKATVPMSVCWLIPQYILFGVSDVFTMVGLQEFFYG 488

Query: 482 QVPSGLRSIGLSLYLSIFGVGNFLSSFLISCIDKLTSGDGKESWFDNNLNKAHLDYFYWL 541
           +VP  LRS+GL+LYLSI G+GNFLSSF++S I++ TS  G+ SWF NNLN+AHLDYFYWL
Sbjct: 489 EVPPQLRSMGLALYLSIIGIGNFLSSFMVSVIEEATSQSGQVSWFSNNLNQAHLDYFYWL 548

Query: 542 LAGLSAVGLAAFLCAARTYIY 557
           LA LS++     +  A++Y+Y
Sbjct: 549 LACLSSLAFIFTVYFAKSYLY 551

BLAST of MC11g_new0505 vs. TAIR 10
Match: AT1G22550.1 (Major facilitator superfamily protein )

HSP 1 Score: 581.6 bits (1498), Expect = 6.6e-166
Identity = 301/561 (53.65%), Postives = 405/561 (72.19%), Query Frame = 0

Query: 2   ESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLTD 61
           E+ L++DS  V  +VD+ G PA +SS+GGWRSA +IIGVEV ERFAY+G+ SNLI +LT 
Sbjct: 7   EAALIEDS--VSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLITYLTG 66

Query: 62  QLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSAT 121
            L QSTATAA NVN  SG AS+LP+LGA +AD++LGRY TI+++S +YIL LGLLT+S+ 
Sbjct: 67  PLGQSTATAAVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYILGLGLLTLSSI 126

Query: 122 FPSPTISAS-LHTEQAS--PSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQ 181
                +S    H   AS  P     ILFF SLYL+A+ QGGH+PCVQAFGADQFD   P+
Sbjct: 127 LILMGLSEQRQHNRNASAKPFFWVNILFFCSLYLVAIGQGGHKPCVQAFGADQFDVGDPK 186

Query: 182 ETQAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRT 241
           E  ++ SFFNWW+  +S    +++ ++ YVQDN++W LGFGIPC+ M+ AL +FL G +T
Sbjct: 187 ERISRGSFFNWWFLSLSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALFLFGRKT 246

Query: 242 YRY--SNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRFLDKA 301
           YRY   +R  + N F RIGRVF+ A +N  L   ++ H  +  V    +   Q  FL KA
Sbjct: 247 YRYPRGDREGKNNAFARIGRVFLVAFKNRKL---KLTHSGQLEVGSYKKCKGQLEFLAKA 306

Query: 302 LVVPHGSKEDSLVCSVSEIEEAKAVLRLGPIWVTCLAYALVFSQAFTFFTKQGVTMDRSV 361
           L+   G  E    CS  ++E+A A++RL PIW+T +   + ++Q  TFFTKQGVT+DR +
Sbjct: 307 LLPGEGGVEP---CSSRDVEDAMALVRLIPIWITSVVSTIPYAQYATFFTKQGVTVDRKI 366

Query: 362 VPGFEVPAASLQSFIGLSVVIFLPIYDRILVPIARTLTGEPSGITMLQRIGIGMLLSALA 421
           +PGFE+P AS Q+ IGLS+ I +P Y+R+ +P+AR +T +PSGITMLQRIG GM+LS+L 
Sbjct: 367 LPGFEIPPASFQALIGLSIFISVPTYERVFLPLARLITKKPSGITMLQRIGAGMVLSSLN 426

Query: 422 MVIAASVEIKRINTAQEYGLVDLPTATVPLSVWWLVPQYALIGVADGFTMVGLQEFFYDQ 481
           MV+AA VE+KR+ TA+E+GLVD P AT+P+S+WW VPQY L+G+ D F++VG QEFFYDQ
Sbjct: 427 MVVAALVEMKRLETAKEHGLVDRPDATIPMSIWWFVPQYLLLGMIDVFSLVGTQEFFYDQ 486

Query: 482 VPSGLRSIGLSLYLSIFGVGNFLSSFLISCIDKLTSGDGKESWFDNNLNKAHLDYFYWLL 541
           VP+ LRSIGL+L LS  G+ +FLS FLI+ I+  T  +G +SWF+ NLN+AH+DYFYWLL
Sbjct: 487 VPTELRSIGLALSLSAMGLASFLSGFLITVINWATGKNGGDSWFNTNLNRAHVDYFYWLL 546

Query: 542 AGLSAVGLAAFLCAARTYIYK 558
           A  +A+G  AFL  +R Y+Y+
Sbjct: 547 AAFTAIGFLAFLLLSRLYVYR 559