MC11g_new0440 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC11g_new0440
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	FAD-binding Berberine family protein
Location	MC11: 17680914 .. 17681575 (-)
RNA-Seq Expression	MC11g_new0440
Synteny	MC11g_new0440

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATAGGGAAGGCGTCGTTGGTGGGATTGTATCTTGGGCCAGTGGAGAAACTTTTGGAGATCGTGAATCAGAAGCCTACTCAGTTTGAAATTGCAGAAACAAGAGTGCAGTGACATGAGTTGGATTCAATCTGTTATGTTTTGGATCAATTTCCCAAACGGTATGGCCTCAGAGGCGTTATTGAGCAGGGAAATCTCATCTCTACCTTTTCTCAAATGGAAATCGGACTATGTGAGGGAACCTATTTCGAGAGAAGGGATAGAATCGATATAGAAGACAATAATTGAAATTAAGGAAGTGGGTCTCGTTTGGAACCCTTACGGAGGCAGCAACGCCATTTCTACACAGAGCAGGAGTGAAATTCAAGCTCCAATACTCTGCAAACTGGAAGGAAGGGGGCGATGAAGTGGCTGAGGAGAATATTGAATTGGCGAGAAGATTGTACAAAGCAATGACGCCTTATGTGTCGAAGAATTCAAGGGAGACTTTCCTGAATTACAAGGACGTCGACATGGGGAGCACCGCCCATTGGAGCGTGGAGGAAGGTAGGGTTTATGGGGCGAAGTATTTCAAGGGAAATTTTGAGAGATTGGTGAGCGGGAAGACGAAGGTTGATCCTGAGAATTTCTTTAGAAATGAACAAAGTATCCCAACTCGTTGA

mRNA sequence

ATGATAGGGAAGGCGTCGTTGGTGGGATTGTATCTTGGGCCAGTGGAGAAACTTTTGGAGATCGTGAATCAGAAGCCTACTCAGTTTGAAATTGCAGAAACAAGAGTGCAGAAGTGGGTCTCGTTTGGAACCCTTACGGAGGCAGCAACGCCATTTCTACACAGAGCAGGAGTGAAATTCAAGCTCCAATACTCTGCAAACTGGAAGGAAGGGGGCGATGAAGTGGCTGAGGAGAATATTGAATTGGCGAGAAGATTGTACAAAGCAATGACGCCTTATGTGTCGAAGAATTCAAGGGAGACTTTCCTGAATTACAAGGACGTCGACATGGGGAGCACCGCCCATTGGAGCGTGGAGGAAGGTAGGGTTTATGGGGCGAAGTATTTCAAGGGAAATTTTGAGAGATTGGTGAGCGGGAAGACGAAGGTTGATCCTGAGAATTTCTTTAGAAATGAACAAAGTATCCCAACTCGTTGA

Coding sequence (CDS)

ATGATAGGGAAGGCGTCGTTGGTGGGATTGTATCTTGGGCCAGTGGAGAAACTTTTGGAGATCGTGAATCAGAAGCCTACTCAGTTTGAAATTGCAGAAACAAGAGTGCAGAAGTGGGTCTCGTTTGGAACCCTTACGGAGGCAGCAACGCCATTTCTACACAGAGCAGGAGTGAAATTCAAGCTCCAATACTCTGCAAACTGGAAGGAAGGGGGCGATGAAGTGGCTGAGGAGAATATTGAATTGGCGAGAAGATTGTACAAAGCAATGACGCCTTATGTGTCGAAGAATTCAAGGGAGACTTTCCTGAATTACAAGGACGTCGACATGGGGAGCACCGCCCATTGGAGCGTGGAGGAAGGTAGGGTTTATGGGGCGAAGTATTTCAAGGGAAATTTTGAGAGATTGGTGAGCGGGAAGACGAAGGTTGATCCTGAGAATTTCTTTAGAAATGAACAAAGTATCCCAACTCGTTGA

Protein sequence

MIGKASLVGLYLGPVEKLLEIVNQKPTQFEIAETRVQKWVSFGTLTEAATPFLHRAGVKFKLQYSANWKEGGDEVAEENIELARRLYKAMTPYVSKNSRETFLNYKDVDMGSTAHWSVEEGRVYGAKYFKGNFERLVSGKTKVDPENFFRNEQSIPTR

Homology

[Close]

BLAST of MC11g_new0440 vs. ExPASy Swiss-Prot
Match: Q9SA88 (Berberine bridge enzyme-like 11 OS=Arabidopsis thaliana OX=3702 GN=At1g30730 PE=2 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 2.0e-32
Identity = 64/116 (55.17%), Postives = 82/116 (70.69%), Query Frame = 0


Query: 43  GTLTEAATPFLHRAGVKFKLQYSANWKEGGDEVAEENIELARRLYKAMTPYVSKNSRETF 102
           G +  + TPF HR G  F ++Y  +W E GD V ++ + LA   Y+ MTPYVS N RE F
Sbjct: 410 GEIPSSRTPFPHRGGNLFNIEYIIDWSEAGDNVEKKYLALANEFYRFMTPYVSSNPREAF 469

Query: 103 LNYKDVDMGSTAHWSVEEGRVYGAKYFKGNFERLVSGKTKVDPENFFRNEQSIPTR 159
           LNY+D+D+GS+ + + EEG++YGAKYFK NFERLV  KTK D  NF+RNEQSIP R
Sbjct: 470 LNYRDIDIGSSGNSTYEEGKIYGAKYFKDNFERLVDIKTKFDEINFWRNEQSIPVR 525

[Close]

BLAST of MC11g_new0440 vs. ExPASy Swiss-Prot
Match: Q9SA87 (Berberine bridge enzyme-like 10 OS=Arabidopsis thaliana OX=3702 GN=At1g30720 PE=2 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 2.6e-32
Identity = 63/116 (54.31%), Postives = 83/116 (71.55%), Query Frame = 0


Query: 43  GTLTEAATPFLHRAGVKFKLQYSANWKEGGDEVAEENIELARRLYKAMTPYVSKNSRETF 102
           G +  + TPF HRAG  F ++Y  +W E GD V ++ + LA   Y+ MTPYVS N RE F
Sbjct: 411 GEIPSSRTPFPHRAGNLFNIEYIIDWSEAGDNVEKKYLALANEFYRFMTPYVSSNPREAF 470

Query: 103 LNYKDVDMGSTAHWSVEEGRVYGAKYFKGNFERLVSGKTKVDPENFFRNEQSIPTR 159
           LNY+D+D+GS+   + +EG++YGAKYFK NFERLV  KT +D ENF++NEQSIP R
Sbjct: 471 LNYRDLDIGSSVKSTYQEGKIYGAKYFKENFERLVDIKTTIDAENFWKNEQSIPVR 526

[Close]

BLAST of MC11g_new0440 vs. ExPASy Swiss-Prot
Match: F4HV09 (Berberine bridge enzyme-like 14 OS=Arabidopsis thaliana OX=3702 GN=At1g34575 PE=3 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.0e-31
Identity = 63/107 (58.88%), Postives = 80/107 (74.77%), Query Frame = 0


Query: 50  TPFLHRAGVKFKLQYSANWKEGGDEVAEENIELARRLYKAMTPYVSKNSRETFLNYKDVD 109
           T F HRAG  FK+QYS+NW   G+E A + +    R+++AM+PYVSKN RE FLNY+D+D
Sbjct: 416 TAFPHRAGNMFKIQYSSNWFVPGEEAASDCLSQTERVFEAMSPYVSKNPREAFLNYRDID 475

Query: 110 MGSTAHWSVEEGRVYGAKYFKGNFERLVSGKTKVDPENFFRNEQSIP 157
           +G   + + EEG+VYG KYFK NFERLV  KT+VDP+N FR EQSIP
Sbjct: 476 IGKNLNSTYEEGKVYGVKYFKNNFERLVQVKTRVDPDNIFRYEQSIP 522

[Close]

BLAST of MC11g_new0440 vs. ExPASy Swiss-Prot
Match: Q9SA86 (Berberine bridge enzyme-like 9 OS=Arabidopsis thaliana OX=3702 GN=At1g30710 PE=2 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 1.3e-31
Identity = 64/107 (59.81%), Postives = 83/107 (77.57%), Query Frame = 0


Query: 50  TPFLHRAGVKFKLQYSANWKEGGDEVAEENIELARRLYKAMTPYVSKNSRETFLNYKDVD 109
           T F HRAG  FK+QY+ANW   G+ VA++ +    RL++AM+PYVSKN RE FLNY+DVD
Sbjct: 420 TAFPHRAGNMFKIQYAANWFVPGEAVAKDCLSQTERLFEAMSPYVSKNPREAFLNYRDVD 479

Query: 110 MGSTAHWSVEEGRVYGAKYFKGNFERLVSGKTKVDPENFFRNEQSIP 157
           +G + + + EEG+VYG KYFK NFE+LV  K++VDP+NFFR EQSIP
Sbjct: 480 IGKSLNSTYEEGKVYGFKYFKDNFEKLVKIKSRVDPDNFFRYEQSIP 526

[Close]

BLAST of MC11g_new0440 vs. ExPASy Swiss-Prot
Match: Q9SA85 (Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 3.6e-29
Identity = 68/143 (47.55%), Postives = 93/143 (65.03%), Query Frame = 0


Query: 19  LEIVNQKPTQFEIAETRVQKW-VSFGTLTEAATPFLHRAGVKFKLQYSANWKEGGDEVAE 78
           LE + +K  + EI       +    G ++   TPF +RAG  +K+QY ANW++  + + +
Sbjct: 384 LESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFPYRAGNLWKIQYGANWRD--ETLTD 443

Query: 79  ENIELARRLYKAMTPYVSKNSRETFLNYKDVDMGSTAH----WSVEEGRVYGAKYFKGNF 138
             +EL R+LY+ MTP+VSKN R++F NY+DVD+G  +H     S  EG+ YG KYF GNF
Sbjct: 444 RYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHNGKISSYVEGKRYGKKYFAGNF 503

Query: 139 ERLVSGKTKVDPENFFRNEQSIP 157
           ERLV  KT+VD  NFFRNEQSIP
Sbjct: 504 ERLVKIKTRVDSGNFFRNEQSIP 524

[Close]

BLAST of MC11g_new0440 vs. NCBI nr
Match: XP_022146700.1 (LOW QUALITY PROTEIN: berberine bridge enzyme-like 14 [Momordica charantia])

HSP 1 Score: 217 bits (552), Expect = 6.32e-65
Identity = 122/227 (53.74%), Postives = 134/227 (59.03%), Query Frame = 0


Query: 1   MIGKASLVGLYLGPVEKLLEIVNQKPTQFEIA--ETRVQKWVS----------------- 60
           M GKASLVGLYLG VEKLLEIVNQ     ++   E R   W+                  
Sbjct: 300 MTGKASLVGLYLGSVEKLLEIVNQNIPSLKLQKQECREMSWIQSTMFWFNFPNGTAPEAL 359

Query: 61  --------------------------------------------------FGTLTEAATP 120
                                                                ++EAATP
Sbjct: 360 LSREIPSLPFLKRKSDYVREPISRDGIESIWKTIIEIKEVSLVWNPYGGRMSQISEAATP 419

Query: 121 FLHRAGVKFKLQYSANWKEGGDEVAEENIELARRLYKAMTPYVSKNSRETFLNYKDVDMG 158
           F HRAGVKFKLQYSANWKE G+E AEENI L RRLY+AMTPYVSKN RE FLNY+D+D+G
Sbjct: 420 FPHRAGVKFKLQYSANWKERGNEEAEENIALTRRLYEAMTPYVSKNPREAFLNYRDIDVG 479

[Close]

BLAST of MC11g_new0440 vs. NCBI nr
Match: XP_022159193.1 (berberine bridge enzyme-like 14 [Momordica charantia])

HSP 1 Score: 217 bits (552), Expect = 6.74e-65
Identity = 121/227 (53.30%), Postives = 133/227 (58.59%), Query Frame = 0


Query: 1   MIGKASLVGLYLGPVEKLLEIVNQKPTQFEIAETRVQK---------WVSF--------- 60
           M GKASLVGLYLGP EKLL IVNQ  +   + +    +         W +F         
Sbjct: 303 MTGKASLVGLYLGPAEKLLGIVNQNISSLRLQKQECSEISXIQSVLFWFNFPNGXAPEAL 362

Query: 61  ---------------------------------------------------GTLTEAATP 120
                                                                ++EAATP
Sbjct: 363 LSREIPSLPFLKRKSDYVREPISREGIEAIWKAVIEIKEVGLVWNPYGGRMSQISEAATP 422

Query: 121 FLHRAGVKFKLQYSANWKEGGDEVAEENIELARRLYKAMTPYVSKNSRETFLNYKDVDMG 158
           F HR GVKFKLQYSANWKEGGDEVAEENI L RRLY+ MTPYVSKN RE FLNY+D+D+G
Sbjct: 423 FPHRXGVKFKLQYSANWKEGGDEVAEENIALVRRLYEVMTPYVSKNPREAFLNYRDIDVG 482

[Close]

BLAST of MC11g_new0440 vs. NCBI nr
Match: XP_038883273.1 (berberine bridge enzyme-like 14 [Benincasa hispida])

HSP 1 Score: 203 bits (517), Expect = 1.05e-59
Identity = 110/225 (48.89%), Postives = 136/225 (60.44%), Query Frame = 0


Query: 3   GKASLVGLYLGPVEKLLEIVNQKPTQFEIAETR---------VQKWVSF--GT------- 62
           GKA+LV L+LGP+EKL+EI+NQ     ++ +           V  W +F  GT       
Sbjct: 306 GKATLVALFLGPLEKLMEIINQNIPSLKLQKEECIEMNWIESVLFWANFENGTAPEALLK 365

Query: 63  ---------------------------------------------------LTEAATPFL 122
                                                              ++E ATPF 
Sbjct: 366 RETPSVQYLKRKSDYVREPISKEGIEAIWKAIMETEEVGLTCNPYGGRMSEISETATPFP 425

Query: 123 HRAGVKFKLQYSANWKEGGDEVAEENIELARRLYKAMTPYVSKNSRETFLNYKDVDMGST 158
           HRAGVKFK+QYS+NWKEGGD+ A+E +ELARRLY+AMTPYV+KN RE FLNY+D+D+GS+
Sbjct: 426 HRAGVKFKMQYSSNWKEGGDQEAKEEMELARRLYEAMTPYVTKNPREAFLNYRDIDVGSS 485

[Close]

BLAST of MC11g_new0440 vs. NCBI nr
Match: XP_038881609.1 (berberine bridge enzyme-like 14 [Benincasa hispida])

HSP 1 Score: 196 bits (497), Expect = 1.08e-56
Identity = 106/225 (47.11%), Postives = 133/225 (59.11%), Query Frame = 0


Query: 3   GKASLVGLYLGPVEKLLEIVNQKPTQFEIAETR---------VQKWVSF--GT------- 62
           GKA+LV L+LGPVEKL++I+NQ     ++             V  W +F  GT       
Sbjct: 303 GKATLVALFLGPVEKLMQIINQNIPSLKLQRQECFEMSWIQSVLFWANFPNGTAPEALLS 362

Query: 63  ---------------------------------------------------LTEAATPFL 122
                                                              ++E  TPF 
Sbjct: 363 RKISSTPFLKRKSDYVREPISREGIEAIWKAIMEVEEVGLTWNPYGGKMSQISETETPFP 422

Query: 123 HRAGVKFKLQYSANWKEGGDEVAEENIELARRLYKAMTPYVSKNSRETFLNYKDVDMGST 158
           HRAGVKFK+QYS+NWKEGGD+ A+E +ELARRLY+AMTPYV+KN RE FLNY+D+D+G +
Sbjct: 423 HRAGVKFKIQYSSNWKEGGDQEAQEEMELARRLYEAMTPYVTKNPREAFLNYRDIDVGIS 482

[Close]

BLAST of MC11g_new0440 vs. NCBI nr
Match: XP_008441073.1 (PREDICTED: flavin-dependent oxidoreductase FOX2-like [Cucumis melo])

HSP 1 Score: 195 bits (495), Expect = 1.76e-56
Identity = 105/225 (46.67%), Postives = 130/225 (57.78%), Query Frame = 0


Query: 3   GKASLVGLYLGPVEKLLEIVNQKPTQFEIAETRV--QKWVS------------------- 62
           GK +LV L+LGPVEKL+EIVNQ     ++ +       W+                    
Sbjct: 303 GKTTLVALFLGPVEKLMEIVNQNIPSLKLQKQECIEMNWIESVLFFANFANGTAPEALLK 362

Query: 63  ------------------------------------------------FGTLTEAATPFL 122
                                                            G ++E ATPF 
Sbjct: 363 RESPSGTYLKRKSDYVREGISKKGIEDIWKLLMEIEGVGLTCNPYGGRMGEISETATPFP 422

Query: 123 HRAGVKFKLQYSANWKEGGDEVAEENIELARRLYKAMTPYVSKNSRETFLNYKDVDMGST 158
           HRAGVKFK+QYSANWK+ G+E A+ENIELAR+LY+AM+PYV+KN RE FLNY+D+D+GS+
Sbjct: 423 HRAGVKFKIQYSANWKKEGEEEAKENIELARKLYEAMSPYVTKNPREAFLNYRDIDVGSS 482

[Close]

BLAST of MC11g_new0440 vs. ExPASy TrEMBL
Match: A0A6J1CY03 (LOW QUALITY PROTEIN: berberine bridge enzyme-like 14 OS=Momordica charantia OX=3673 GN=LOC111015841 PE=3 SV=1)

HSP 1 Score: 217 bits (552), Expect = 3.06e-65
Identity = 122/227 (53.74%), Postives = 134/227 (59.03%), Query Frame = 0


Query: 1   MIGKASLVGLYLGPVEKLLEIVNQKPTQFEIA--ETRVQKWVS----------------- 60
           M GKASLVGLYLG VEKLLEIVNQ     ++   E R   W+                  
Sbjct: 300 MTGKASLVGLYLGSVEKLLEIVNQNIPSLKLQKQECREMSWIQSTMFWFNFPNGTAPEAL 359

Query: 61  --------------------------------------------------FGTLTEAATP 120
                                                                ++EAATP
Sbjct: 360 LSREIPSLPFLKRKSDYVREPISRDGIESIWKTIIEIKEVSLVWNPYGGRMSQISEAATP 419

Query: 121 FLHRAGVKFKLQYSANWKEGGDEVAEENIELARRLYKAMTPYVSKNSRETFLNYKDVDMG 158
           F HRAGVKFKLQYSANWKE G+E AEENI L RRLY+AMTPYVSKN RE FLNY+D+D+G
Sbjct: 420 FPHRAGVKFKLQYSANWKERGNEEAEENIALTRRLYEAMTPYVSKNPREAFLNYRDIDVG 479

[Close]

BLAST of MC11g_new0440 vs. ExPASy TrEMBL
Match: A0A6J1DY47 (berberine bridge enzyme-like 14 OS=Momordica charantia OX=3673 GN=LOC111025613 PE=3 SV=1)

HSP 1 Score: 217 bits (552), Expect = 3.27e-65
Identity = 121/227 (53.30%), Postives = 133/227 (58.59%), Query Frame = 0


Query: 1   MIGKASLVGLYLGPVEKLLEIVNQKPTQFEIAETRVQK---------WVSF--------- 60
           M GKASLVGLYLGP EKLL IVNQ  +   + +    +         W +F         
Sbjct: 303 MTGKASLVGLYLGPAEKLLGIVNQNISSLRLQKQECSEISXIQSVLFWFNFPNGXAPEAL 362

Query: 61  ---------------------------------------------------GTLTEAATP 120
                                                                ++EAATP
Sbjct: 363 LSREIPSLPFLKRKSDYVREPISREGIEAIWKAVIEIKEVGLVWNPYGGRMSQISEAATP 422

Query: 121 FLHRAGVKFKLQYSANWKEGGDEVAEENIELARRLYKAMTPYVSKNSRETFLNYKDVDMG 158
           F HR GVKFKLQYSANWKEGGDEVAEENI L RRLY+ MTPYVSKN RE FLNY+D+D+G
Sbjct: 423 FPHRXGVKFKLQYSANWKEGGDEVAEENIALVRRLYEVMTPYVSKNPREAFLNYRDIDVG 482

[Close]

BLAST of MC11g_new0440 vs. ExPASy TrEMBL
Match: A0A0A0KKY2 (BBE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G525690 PE=4 SV=1)

HSP 1 Score: 190 bits (483), Expect = 5.32e-58
Identity = 108/225 (48.00%), Postives = 134/225 (59.56%), Query Frame = 0


Query: 3   GKASLVGLYLGPVEKLLEIVNQ--------KPTQFEIAETR-VQKWVSF--GT------- 62
           GKA+LV L+LGPVEK+++I+NQ        K   FE++  + V  W +F  GT       
Sbjct: 26  GKATLVALFLGPVEKVMDIMNQNIPSLKLQKQECFEMSWIQSVLFWANFPSGTAPEALLS 85

Query: 63  ---------------------------------------------------LTEAATPFL 122
                                                              ++E ATPF 
Sbjct: 86  RQMASTPYLKRKSDYVREPISREGVEAIWKALMDVEEVGLTWNPYGGRMSEISETATPFP 145

Query: 123 HRAGVKFKLQYSANWKEGGDEVAEENIELARRLYKAMTPYVSKNSRETFLNYKDVDMGST 158
           HRAGVKFK+QYS+NWKE GD  AEE I L+R+LY+AMTP+VSKN RE FLNY+D+D+GS+
Sbjct: 146 HRAGVKFKIQYSSNWKEAGDTEAEEEIALSRKLYEAMTPFVSKNPREAFLNYRDIDIGSS 205

[Close]

BLAST of MC11g_new0440 vs. ExPASy TrEMBL
Match: A0A1S3B2M2 (flavin-dependent oxidoreductase FOX2-like OS=Cucumis melo OX=3656 GN=LOC103485297 PE=3 SV=1)

HSP 1 Score: 195 bits (495), Expect = 8.51e-57
Identity = 105/225 (46.67%), Postives = 130/225 (57.78%), Query Frame = 0


Query: 3   GKASLVGLYLGPVEKLLEIVNQKPTQFEIAETRV--QKWVS------------------- 62
           GK +LV L+LGPVEKL+EIVNQ     ++ +       W+                    
Sbjct: 303 GKTTLVALFLGPVEKLMEIVNQNIPSLKLQKQECIEMNWIESVLFFANFANGTAPEALLK 362

Query: 63  ------------------------------------------------FGTLTEAATPFL 122
                                                            G ++E ATPF 
Sbjct: 363 RESPSGTYLKRKSDYVREGISKKGIEDIWKLLMEIEGVGLTCNPYGGRMGEISETATPFP 422

Query: 123 HRAGVKFKLQYSANWKEGGDEVAEENIELARRLYKAMTPYVSKNSRETFLNYKDVDMGST 158
           HRAGVKFK+QYSANWK+ G+E A+ENIELAR+LY+AM+PYV+KN RE FLNY+D+D+GS+
Sbjct: 423 HRAGVKFKIQYSANWKKEGEEEAKENIELARKLYEAMSPYVTKNPREAFLNYRDIDVGSS 482

[Close]

BLAST of MC11g_new0440 vs. ExPASy TrEMBL
Match: A0A5A7UD95 (Flavin-dependent oxidoreductase FOX2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G009810 PE=3 SV=1)

HSP 1 Score: 192 bits (487), Expect = 1.26e-55
Identity = 106/225 (47.11%), Postives = 130/225 (57.78%), Query Frame = 0


Query: 3   GKASLVGLYLGPVEKLLEIVNQKPTQFEIAETR---------VQKWVSF--GT------- 62
           GKA+LV L+LGPVEKL+EI+NQ     ++             V  W +F  GT       
Sbjct: 302 GKATLVSLFLGPVEKLMEIMNQNIPSLKLQREECFEMSWIQSVLFWANFANGTAPEALLS 361

Query: 63  ---------------------------------------------------LTEAATPFL 122
                                                              ++E ATPF 
Sbjct: 362 RKMASTPYLKRKSDYVREPISREGIEGIWKAIMDVEKVGLTWNPYGGRMSEISETATPFP 421

Query: 123 HRAGVKFKLQYSANWKEGGDEVAEENIELARRLYKAMTPYVSKNSRETFLNYKDVDMGST 158
           HRAGVKFK+QYS+NWKE GDE AEE IE +R+LY+AM+PYV+ N RE FLNY+D+D+GS+
Sbjct: 422 HRAGVKFKIQYSSNWKEAGDEEAEEEIESSRKLYEAMSPYVTNNPREAFLNYRDIDVGSS 481

[Close]

BLAST of MC11g_new0440 vs. TAIR 10
Match: AT1G30730.1 (FAD-binding Berberine family protein )

HSP 1 Score: 140.2 bits (352), Expect = 1.4e-33
Identity = 64/116 (55.17%), Postives = 82/116 (70.69%), Query Frame = 0


Query: 43  GTLTEAATPFLHRAGVKFKLQYSANWKEGGDEVAEENIELARRLYKAMTPYVSKNSRETF 102
           G +  + TPF HR G  F ++Y  +W E GD V ++ + LA   Y+ MTPYVS N RE F
Sbjct: 410 GEIPSSRTPFPHRGGNLFNIEYIIDWSEAGDNVEKKYLALANEFYRFMTPYVSSNPREAF 469

Query: 103 LNYKDVDMGSTAHWSVEEGRVYGAKYFKGNFERLVSGKTKVDPENFFRNEQSIPTR 159
           LNY+D+D+GS+ + + EEG++YGAKYFK NFERLV  KTK D  NF+RNEQSIP R
Sbjct: 470 LNYRDIDIGSSGNSTYEEGKIYGAKYFKDNFERLVDIKTKFDEINFWRNEQSIPVR 525

[Close]

BLAST of MC11g_new0440 vs. TAIR 10
Match: AT1G30720.1 (FAD-binding Berberine family protein )

HSP 1 Score: 139.8 bits (351), Expect = 1.9e-33
Identity = 63/116 (54.31%), Postives = 83/116 (71.55%), Query Frame = 0


Query: 43  GTLTEAATPFLHRAGVKFKLQYSANWKEGGDEVAEENIELARRLYKAMTPYVSKNSRETF 102
           G +  + TPF HRAG  F ++Y  +W E GD V ++ + LA   Y+ MTPYVS N RE F
Sbjct: 411 GEIPSSRTPFPHRAGNLFNIEYIIDWSEAGDNVEKKYLALANEFYRFMTPYVSSNPREAF 470

Query: 103 LNYKDVDMGSTAHWSVEEGRVYGAKYFKGNFERLVSGKTKVDPENFFRNEQSIPTR 159
           LNY+D+D+GS+   + +EG++YGAKYFK NFERLV  KT +D ENF++NEQSIP R
Sbjct: 471 LNYRDLDIGSSVKSTYQEGKIYGAKYFKENFERLVDIKTTIDAENFWKNEQSIPVR 526

[Close]

BLAST of MC11g_new0440 vs. TAIR 10
Match: AT1G34575.1 (FAD-binding Berberine family protein )

HSP 1 Score: 137.9 bits (346), Expect = 7.1e-33
Identity = 63/107 (58.88%), Postives = 80/107 (74.77%), Query Frame = 0


Query: 50  TPFLHRAGVKFKLQYSANWKEGGDEVAEENIELARRLYKAMTPYVSKNSRETFLNYKDVD 109
           T F HRAG  FK+QYS+NW   G+E A + +    R+++AM+PYVSKN RE FLNY+D+D
Sbjct: 416 TAFPHRAGNMFKIQYSSNWFVPGEEAASDCLSQTERVFEAMSPYVSKNPREAFLNYRDID 475

Query: 110 MGSTAHWSVEEGRVYGAKYFKGNFERLVSGKTKVDPENFFRNEQSIP 157
           +G   + + EEG+VYG KYFK NFERLV  KT+VDP+N FR EQSIP
Sbjct: 476 IGKNLNSTYEEGKVYGVKYFKNNFERLVQVKTRVDPDNIFRYEQSIP 522

[Close]

BLAST of MC11g_new0440 vs. TAIR 10
Match: AT1G30710.1 (FAD-binding Berberine family protein )

HSP 1 Score: 137.5 bits (345), Expect = 9.3e-33
Identity = 64/107 (59.81%), Postives = 83/107 (77.57%), Query Frame = 0


Query: 50  TPFLHRAGVKFKLQYSANWKEGGDEVAEENIELARRLYKAMTPYVSKNSRETFLNYKDVD 109
           T F HRAG  FK+QY+ANW   G+ VA++ +    RL++AM+PYVSKN RE FLNY+DVD
Sbjct: 420 TAFPHRAGNMFKIQYAANWFVPGEAVAKDCLSQTERLFEAMSPYVSKNPREAFLNYRDVD 479

Query: 110 MGSTAHWSVEEGRVYGAKYFKGNFERLVSGKTKVDPENFFRNEQSIP 157
           +G + + + EEG+VYG KYFK NFE+LV  K++VDP+NFFR EQSIP
Sbjct: 480 IGKSLNSTYEEGKVYGFKYFKDNFEKLVKIKSRVDPDNFFRYEQSIP 526

[Close]

BLAST of MC11g_new0440 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 129.4 bits (324), Expect = 2.5e-30
Identity = 68/143 (47.55%), Postives = 93/143 (65.03%), Query Frame = 0


Query: 19  LEIVNQKPTQFEIAETRVQKW-VSFGTLTEAATPFLHRAGVKFKLQYSANWKEGGDEVAE 78
           LE + +K  + EI       +    G ++   TPF +RAG  +K+QY ANW++  + + +
Sbjct: 384 LESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFPYRAGNLWKIQYGANWRD--ETLTD 443

Query: 79  ENIELARRLYKAMTPYVSKNSRETFLNYKDVDMGSTAH----WSVEEGRVYGAKYFKGNF 138
             +EL R+LY+ MTP+VSKN R++F NY+DVD+G  +H     S  EG+ YG KYF GNF
Sbjct: 444 RYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHNGKISSYVEGKRYGKKYFAGNF 503

Query: 139 ERLVSGKTKVDPENFFRNEQSIP 157
           ERLV  KT+VD  NFFRNEQSIP
Sbjct: 504 ERLVKIKTRVDSGNFFRNEQSIP 524

The following BLAST results are available for this feature:

BLAST of MC11g_new0440 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SA88	2.0e-32	55.17	Berberine bridge enzyme-like 11 OS=Arabidopsis thaliana OX=3702 GN=At1g30730 PE=...	[more]
Q9SA87	2.6e-32	54.31	Berberine bridge enzyme-like 10 OS=Arabidopsis thaliana OX=3702 GN=At1g30720 PE=...	[more]
F4HV09	1.0e-31	58.88	Berberine bridge enzyme-like 14 OS=Arabidopsis thaliana OX=3702 GN=At1g34575 PE=...	[more]
Q9SA86	1.3e-31	59.81	Berberine bridge enzyme-like 9 OS=Arabidopsis thaliana OX=3702 GN=At1g30710 PE=2...	[more]
Q9SA85	3.6e-29	47.55	Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2...	[more]

BLAST of MC11g_new0440 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146700.1	6.32e-65	53.74	LOW QUALITY PROTEIN: berberine bridge enzyme-like 14 [Momordica charantia]	[more]
XP_022159193.1	6.74e-65	53.30	berberine bridge enzyme-like 14 [Momordica charantia]	[more]
XP_038883273.1	1.05e-59	48.89	berberine bridge enzyme-like 14 [Benincasa hispida]	[more]
XP_038881609.1	1.08e-56	47.11	berberine bridge enzyme-like 14 [Benincasa hispida]	[more]
XP_008441073.1	1.76e-56	46.67	PREDICTED: flavin-dependent oxidoreductase FOX2-like [Cucumis melo]	[more]

BLAST of MC11g_new0440 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CY03	3.06e-65	53.74	LOW QUALITY PROTEIN: berberine bridge enzyme-like 14 OS=Momordica charantia OX=3...	[more]
A0A6J1DY47	3.27e-65	53.30	berberine bridge enzyme-like 14 OS=Momordica charantia OX=3673 GN=LOC111025613 P...	[more]
A0A0A0KKY2	5.32e-58	48.00	BBE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G525690 PE=4 SV...	[more]
A0A1S3B2M2	8.51e-57	46.67	flavin-dependent oxidoreductase FOX2-like OS=Cucumis melo OX=3656 GN=LOC10348529...	[more]
A0A5A7UD95	1.26e-55	47.11	Flavin-dependent oxidoreductase FOX2-like OS=Cucumis melo var. makuwa OX=1194695...	[more]

BLAST of MC11g_new0440 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G30730.1	1.4e-33	55.17	FAD-binding Berberine family protein	[more]
AT1G30720.1	1.9e-33	54.31	FAD-binding Berberine family protein	[more]
AT1G34575.1	7.1e-33	58.88	FAD-binding Berberine family protein	[more]
AT1G30710.1	9.3e-33	59.81	FAD-binding Berberine family protein	[more]
AT1G30700.1	2.5e-30	47.55	FAD-binding Berberine family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.462.20		coord: 45..97 e-value: 5.9E-39 score: 136.3
None	No IPR available	PANTHER	PTHR32448:SF68	BERBERINE BRIDGE ENZYME-LIKE 14-RELATED	coord: 45..157
None	No IPR available	PANTHER	PTHR32448	OS08G0158400 PROTEIN	coord: 45..157
IPR016169	FAD-binding, type PCMH, subdomain 2	GENE3D	3.30.465.10		coord: 98..156 e-value: 5.9E-39 score: 136.3
IPR012951	Berberine/berberine-like	PFAM	PF08031	BBE	coord: 102..156 e-value: 1.2E-20 score: 73.3

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC11g_new0440.1	MC11g_new0440.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0071949	FAD binding
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0050660	flavin adenine dinucleotide binding