MC11g_new0433 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC11g_new0433
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionRetrovirus-related Pol polyprotein from transposon TNT 1-94
LocationMC11: 17461651 .. 17462280 (+)
RNA-Seq ExpressionMC11g_new0433
SyntenyMC11g_new0433
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GGATGATAGGCGGAGCGTATCAGCAAATGTGTTTACTCTAGGATCAGGAGTAATCACCTGGAGTTCAAAAAAACAAGCCACGACGGCATTGTCGTCTTCGGAAGTAGAGTTCGTCGTAGCAACTTCAGCCGTGTGTCAAGCAATTTGGATAAGAAGAATACTAGCGGAGCTTCAACAGGAGCAAGAGGGAGTAATGGAGATATTCTGCGACAACAAAGCAACGATCTCAATGACGAAGAATCCAACATTTCATAGCAGGACGAAGCACATTGATATTTGTTTCCATTTTATCCGTGATTTGGTTACGAAGGGAGATATTGCTCTAAAGTATTGTAGCACGCACGAACAATGGGCCGACATACTTATGAAGTCTTTGTCAAAGGAGAAATTCTATTACTTTAGAGCGTTGCTTGGAGTTTGCAAATTTGAATCAAGAGGGAGTATTGAAGGAATGATTCAAACTTAGTGGCACCAAATATGGTGGCACTAAGAAAGTGAGATAATGAATAGTGGTACCAATTATGGTGGCACTAAGTTAGTGGGATGATGACTTTTAAGTTTAGGGAGTACTACATTAGTGGAGTTAGTGGTCATGTATACATGTTTTTATTGTTGTAAGCCTATATAAAC

mRNA sequence

ATGGAGATATTCTGCGACAACAAAGCAACGATCTCAATGACGAAGAATCCAACATTTCATAGCAGGACGAAGCACATTGATATTTGTTTCCATTTTATCCGTGATTTGGTTACGAAGGGAGATATTGCTCTAAAGTATTGTAGCACGCACGAACAATGGGCCGACATACTTATGAAGTCTTTGTCAAAGGAGAAATTCTATTACTTTAGAGCGTTGCTTGGAGTTTGCAAATTTGAATCAAGAGGGAGTATTGAAGGAATGATTCAAACTTAG

Coding sequence (CDS)

ATGGAGATATTCTGCGACAACAAAGCAACGATCTCAATGACGAAGAATCCAACATTTCATAGCAGGACGAAGCACATTGATATTTGTTTCCATTTTATCCGTGATTTGGTTACGAAGGGAGATATTGCTCTAAAGTATTGTAGCACGCACGAACAATGGGCCGACATACTTATGAAGTCTTTGTCAAAGGAGAAATTCTATTACTTTAGAGCGTTGCTTGGAGTTTGCAAATTTGAATCAAGAGGGAGTATTGAAGGAATGATTCAAACTTAG

Protein sequence

MEIFCDNKATISMTKNPTFHSRTKHIDICFHFIRDLVTKGDIALKYCSTHEQWADILMKSLSKEKFYYFRALLGVCKFESRGSIEGMIQT
Homology
BLAST of MC11g_new0433 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 2.9e-12
Identity = 34/75 (45.33%), Postives = 46/75 (61.33%), Query Frame = 0

Query: 3    IFCDNKATISMTKNPTFHSRTKHIDICFHFIRDLVTKGDIALKYCSTHEQWADILMKSLS 62
            I+CDN     +  NP FHSR KHI I +HFIR+ V  G + + + STH+Q AD L K LS
Sbjct: 1388 IYCDNVGATYLCANPVFHSRMKHIAIDYHFIRNQVQSGALRVVHVSTHDQLADTLTKPLS 1447

Query: 63   KEKFYYFRALLGVCK 78
            +  F  F + +GV +
Sbjct: 1448 RTAFQNFASKIGVTR 1462

BLAST of MC11g_new0433 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 5.0e-12
Identity = 34/75 (45.33%), Postives = 45/75 (60.00%), Query Frame = 0

Query: 3    IFCDNKATISMTKNPTFHSRTKHIDICFHFIRDLVTKGDIALKYCSTHEQWADILMKSLS 62
            I+CDN     +  NP FHSR KHI + +HFIR+ V  G + + + STH+Q AD L K LS
Sbjct: 1371 IYCDNVGATYLCANPVFHSRMKHIALDYHFIRNQVQSGALRVVHVSTHDQLADTLTKPLS 1430

Query: 63   KEKFYYFRALLGVCK 78
            +  F  F   +GV K
Sbjct: 1431 RVAFQNFSRKIGVIK 1445

BLAST of MC11g_new0433 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 2.7e-10
Identity = 27/73 (36.99%), Postives = 50/73 (68.49%), Query Frame = 0

Query: 3    IFCDNKATISMTKNPTFHSRTKHIDICFHFIRDLVTKGDIALKYCSTHEQWADILMKSLS 62
            ++CD+++ I ++KN  +H+RTKHID+ +H+IR++V    + +   ST+E  AD+L K + 
Sbjct: 1253 VYCDSQSAIDLSKNSMYHARTKHIDVRYHWIREMVDDESLKVLKISTNENPADMLTKVVP 1312

Query: 63   KEKFYYFRALLGV 76
            + KF   + L+G+
Sbjct: 1313 RNKFELCKELVGM 1325

BLAST of MC11g_new0433 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 64.7 bits (156), Expect = 6.1e-10
Identity = 32/75 (42.67%), Postives = 43/75 (57.33%), Query Frame = 0

Query: 1    MEIFCDNKATISMTKNPTFHSRTKHIDICFHFIRDLVTKGDIALKYCSTHEQWADILMKS 60
            ++I+ DN+  IS+  NP+ H R KHIDI +HF R+ V    I L+Y  T  Q ADI  K 
Sbjct: 1326 IKIYEDNQGCISIANNPSCHKRAKHIDIKYHFAREQVQNNVICLEYIPTENQLADIFTKP 1385

Query: 61   LSKEKFYYFRALLGV 76
            L   +F   R  LG+
Sbjct: 1386 LPAARFVELRDKLGL 1400

BLAST of MC11g_new0433 vs. NCBI nr
Match: KAA0036164.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa] >TYK19710.1 Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa])

HSP 1 Score: 146 bits (368), Expect = 2.03e-43
Identity = 67/83 (80.72%), Postives = 75/83 (90.36%), Query Frame = 0

Query: 3  IFCDNKATISMTKNPTFHSRTKHIDICFHFIRDLVTKGDIALKYCSTHEQWADILMKSLS 62
          IFCDNKATISMTKNPTF SRTKHIDI FHFIRDL+ K +++L YCSTHEQWADIL K+LS
Sbjct: 15 IFCDNKATISMTKNPTFRSRTKHIDIRFHFIRDLLAKEEVSLSYCSTHEQWADILTKALS 74

Query: 63 KEKFYYFRALLGVCKFESRGSIE 85
          KEKF YFRA++G+ KFESRGSIE
Sbjct: 75 KEKFCYFRAMMGISKFESRGSIE 97

BLAST of MC11g_new0433 vs. NCBI nr
Match: TYH18023.1 (hypothetical protein ES288_A05G236600v1 [Gossypium darwinii])

HSP 1 Score: 142 bits (358), Expect = 4.74e-42
Identity = 65/84 (77.38%), Postives = 74/84 (88.10%), Query Frame = 0

Query: 1  MEIFCDNKATISMTKNPTFHSRTKHIDICFHFIRDLVTKGDIALKYCSTHEQWADILMKS 60
          MEIFCDNK TI+MTKNPTFH+RTKH+DI +HFIRDLV  G+I L+Y ST +Q AD+L KS
Sbjct: 1  MEIFCDNKETITMTKNPTFHNRTKHVDIRYHFIRDLVANGEITLEYYSTQDQLADVLTKS 60

Query: 61 LSKEKFYYFRALLGVCKFESRGSI 84
          LSKEKFYYFR LLGVCKFESRGS+
Sbjct: 61 LSKEKFYYFRTLLGVCKFESRGSV 84

BLAST of MC11g_new0433 vs. NCBI nr
Match: KAA0054938.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa] >TYK22727.1 Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa])

HSP 1 Score: 144 bits (362), Expect = 3.03e-41
Identity = 67/83 (80.72%), Postives = 75/83 (90.36%), Query Frame = 0

Query: 3   IFCDNKATISMTKNPTFHSRTKHIDICFHFIRDLVTKGDIALKYCSTHEQWADILMKSLS 62
           IFCDNKATIS TKN TFHSRTKHIDI FHFIRDLV K +++L YCSTHEQWADIL+K+LS
Sbjct: 111 IFCDNKATISRTKNLTFHSRTKHIDIRFHFIRDLVAKEEVSLTYCSTHEQWADILIKALS 170

Query: 63  KEKFYYFRALLGVCKFESRGSIE 85
           KEKF YFRA++G+ KFESRGSIE
Sbjct: 171 KEKFCYFRAMMGIRKFESRGSIE 193

BLAST of MC11g_new0433 vs. NCBI nr
Match: KAA0038903.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa])

HSP 1 Score: 144 bits (364), Expect = 3.96e-41
Identity = 67/83 (80.72%), Postives = 74/83 (89.16%), Query Frame = 0

Query: 3   IFCDNKATISMTKNPTFHSRTKHIDICFHFIRDLVTKGDIALKYCSTHEQWADILMKSLS 62
           IFCDNKATISMTKN TFHSRTKHIDI FHFIRDLV + + +L YCSTHEQWADIL K+LS
Sbjct: 145 IFCDNKATISMTKNSTFHSRTKHIDIHFHFIRDLVAEEEFSLSYCSTHEQWADILTKALS 204

Query: 63  KEKFYYFRALLGVCKFESRGSIE 85
           KEKF YFRA++G+ KFESRGSIE
Sbjct: 205 KEKFCYFRAMMGISKFESRGSIE 227

BLAST of MC11g_new0433 vs. NCBI nr
Match: KAA0058475.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa] >TYK07272.1 Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa])

HSP 1 Score: 144 bits (362), Expect = 4.28e-41
Identity = 65/83 (78.31%), Postives = 74/83 (89.16%), Query Frame = 0

Query: 3   IFCDNKATISMTKNPTFHSRTKHIDICFHFIRDLVTKGDIALKYCSTHEQWADILMKSLS 62
           IFCDNKATISM KNPTFHS+TKHIDI FHFIRDLV K +++L YCSTHEQW DIL K+LS
Sbjct: 123 IFCDNKATISMMKNPTFHSQTKHIDIRFHFIRDLVAKEEVSLSYCSTHEQWTDILTKTLS 182

Query: 63  KEKFYYFRALLGVCKFESRGSIE 85
           KEKF YFRA++G+ KFESRG+IE
Sbjct: 183 KEKFCYFRAMMGISKFESRGTIE 205

BLAST of MC11g_new0433 vs. ExPASy TrEMBL
Match: A0A5D3D8B6 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold214G00060 PE=4 SV=1)

HSP 1 Score: 146 bits (368), Expect = 9.83e-44
Identity = 67/83 (80.72%), Postives = 75/83 (90.36%), Query Frame = 0

Query: 3  IFCDNKATISMTKNPTFHSRTKHIDICFHFIRDLVTKGDIALKYCSTHEQWADILMKSLS 62
          IFCDNKATISMTKNPTF SRTKHIDI FHFIRDL+ K +++L YCSTHEQWADIL K+LS
Sbjct: 15 IFCDNKATISMTKNPTFRSRTKHIDIRFHFIRDLLAKEEVSLSYCSTHEQWADILTKALS 74

Query: 63 KEKFYYFRALLGVCKFESRGSIE 85
          KEKF YFRA++G+ KFESRGSIE
Sbjct: 75 KEKFCYFRAMMGISKFESRGSIE 97

BLAST of MC11g_new0433 vs. ExPASy TrEMBL
Match: A0A5D2GJD5 (Uncharacterized protein OS=Gossypium darwinii OX=34276 GN=ES288_A05G236600v1 PE=4 SV=1)

HSP 1 Score: 142 bits (358), Expect = 2.30e-42
Identity = 65/84 (77.38%), Postives = 74/84 (88.10%), Query Frame = 0

Query: 1  MEIFCDNKATISMTKNPTFHSRTKHIDICFHFIRDLVTKGDIALKYCSTHEQWADILMKS 60
          MEIFCDNK TI+MTKNPTFH+RTKH+DI +HFIRDLV  G+I L+Y ST +Q AD+L KS
Sbjct: 1  MEIFCDNKETITMTKNPTFHNRTKHVDIRYHFIRDLVANGEITLEYYSTQDQLADVLTKS 60

Query: 61 LSKEKFYYFRALLGVCKFESRGSI 84
          LSKEKFYYFR LLGVCKFESRGS+
Sbjct: 61 LSKEKFYYFRTLLGVCKFESRGSV 84

BLAST of MC11g_new0433 vs. ExPASy TrEMBL
Match: A0A5A7UIY6 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1163G00510 PE=4 SV=1)

HSP 1 Score: 144 bits (362), Expect = 1.47e-41
Identity = 67/83 (80.72%), Postives = 75/83 (90.36%), Query Frame = 0

Query: 3   IFCDNKATISMTKNPTFHSRTKHIDICFHFIRDLVTKGDIALKYCSTHEQWADILMKSLS 62
           IFCDNKATIS TKN TFHSRTKHIDI FHFIRDLV K +++L YCSTHEQWADIL+K+LS
Sbjct: 111 IFCDNKATISRTKNLTFHSRTKHIDIRFHFIRDLVAKEEVSLTYCSTHEQWADILIKALS 170

Query: 63  KEKFYYFRALLGVCKFESRGSIE 85
           KEKF YFRA++G+ KFESRGSIE
Sbjct: 171 KEKFCYFRAMMGIRKFESRGSIE 193

BLAST of MC11g_new0433 vs. ExPASy TrEMBL
Match: A0A5A7T7G8 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1170G00710 PE=4 SV=1)

HSP 1 Score: 144 bits (364), Expect = 1.92e-41
Identity = 67/83 (80.72%), Postives = 74/83 (89.16%), Query Frame = 0

Query: 3   IFCDNKATISMTKNPTFHSRTKHIDICFHFIRDLVTKGDIALKYCSTHEQWADILMKSLS 62
           IFCDNKATISMTKN TFHSRTKHIDI FHFIRDLV + + +L YCSTHEQWADIL K+LS
Sbjct: 145 IFCDNKATISMTKNSTFHSRTKHIDIHFHFIRDLVAEEEFSLSYCSTHEQWADILTKALS 204

Query: 63  KEKFYYFRALLGVCKFESRGSIE 85
           KEKF YFRA++G+ KFESRGSIE
Sbjct: 205 KEKFCYFRAMMGISKFESRGSIE 227

BLAST of MC11g_new0433 vs. ExPASy TrEMBL
Match: A0A5A7UXY4 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold606G001700 PE=4 SV=1)

HSP 1 Score: 144 bits (362), Expect = 2.07e-41
Identity = 65/83 (78.31%), Postives = 74/83 (89.16%), Query Frame = 0

Query: 3   IFCDNKATISMTKNPTFHSRTKHIDICFHFIRDLVTKGDIALKYCSTHEQWADILMKSLS 62
           IFCDNKATISM KNPTFHS+TKHIDI FHFIRDLV K +++L YCSTHEQW DIL K+LS
Sbjct: 123 IFCDNKATISMMKNPTFHSQTKHIDIRFHFIRDLVAKEEVSLSYCSTHEQWTDILTKTLS 182

Query: 63  KEKFYYFRALLGVCKFESRGSIE 85
           KEKF YFRA++G+ KFESRG+IE
Sbjct: 183 KEKFCYFRAMMGISKFESRGTIE 205

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94HW22.9e-1245.33Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O... [more]
Q9ZT945.0e-1245.33Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O... [more]
P109782.7e-1036.99Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
P041466.1e-1042.67Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3[more]
Match NameE-valueIdentityDescription
KAA0036164.12.03e-4380.72Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. m... [more]
TYH18023.14.74e-4277.38hypothetical protein ES288_A05G236600v1 [Gossypium darwinii][more]
KAA0054938.13.03e-4180.72Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. m... [more]
KAA0038903.13.96e-4180.72Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. m... [more]
KAA0058475.14.28e-4178.31Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. m... [more]
Match NameE-valueIdentityDescription
A0A5D3D8B69.83e-4480.72Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var.... [more]
A0A5D2GJD52.30e-4277.38Uncharacterized protein OS=Gossypium darwinii OX=34276 GN=ES288_A05G236600v1 PE=... [more]
A0A5A7UIY61.47e-4180.72Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var.... [more]
A0A5A7T7G81.92e-4180.72Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var.... [more]
A0A5A7UXY42.07e-4178.31Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var.... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR11439:SF355CYSTEINE-RICH RLK (RECEPTOR-LIKE PROTEIN KINASE) 8coord: 3..77
NoneNo IPR availablePANTHERPTHR11439GAG-POL-RELATED RETROTRANSPOSONcoord: 3..77
NoneNo IPR availableCDDcd09272RNase_HI_RT_Ty1coord: 1..64
e-value: 3.08324E-34
score: 112.176

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC11g_new0433.1MC11g_new0433.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050832 defense response to fungus
biological_process GO:0015074 DNA integration
biological_process GO:0006955 immune response
cellular_component GO:0005886 plasma membrane
molecular_function GO:0052923 all-trans-nonaprenyl-diphosphate synthase (geranyl-diphosphate specific) activity
molecular_function GO:0052924 all-trans-nonaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific) activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0003676 nucleic acid binding