MC11g_new0432 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC11g_new0432
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionResistance gene-like protein
LocationMC11: 17449158 .. 17452141 (-)
RNA-Seq ExpressionMC11g_new0432
SyntenyMC11g_new0432
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGTGTCCTAAATTAGTAGCTCCTGGCAAGTACAAGACTTTAGTTGAAATGTCACGCCGATTGGATAAAATGAATAAGCTCCTTGATTTTGATTTAGAATTGAATGATGTACGTTTTGTAGGAATAGTAGGGATGGGCGGTATTGGTAAGACAACTATTGCTGAAGTTGTTTATGACGACATTTTACTTGGATTTTGGAAGAACAGCTGCTTTCTTCGCATAACTCCGGAGCGCAATTTAGTCACTTTGCAACATCAGCTACTTTCCCAACTTCTTTTAGAAAAAGATATTGAAATTCGGGATGAGGATCAAGGAGCACAACTAATTAAGAATCGTTTGAGTGGTATAAAAGTTCTTATTGTTCTCGATGGAGTCAACAACAAAGGGCAATTAGAAAAGTTAGCTGGAAGTCCCGATTGGTTTGGTCCAAACAGTCGCATCATCATTACCACTAGAAACAAAGACATTCTTCGTCAACCTAATTATGAAGGTAAAATACAAGAATACCATGTGGAGTTACTTGGTTAGAAGGGTGCATTCTCACTCTTTTGCAAGCATGCATTTGGAGATGGTCGTCCTCTCGATGATAATTTAAAAGATCTTTGTAATGGAATTGTAAGACAAACTAGAAGACTTCCATTAGCTTTGATAAAGATTGGATCCTCTTTGTACGGTCAAAGTATAGACATATGGAGAGAAGAGCTTGAGAGTCTCACTAGAGCTCACAATAAACGCCTGTTTGACACATTAAAGATAAGTTTTGATGATATAGGAAAAACCAGTCAACAAGTTTTTCTTGATTTTGCTTGTTTCTTCAATGGAAAAGCAATAGATAGAGTGATTGAAATACTTAGGAGTTTAGGTTACATGCCGCACAGTGAGATGCAATTGTTGTGTGATAGATGTCTTATCGAAGTTGGACCCCAAAAAACAATAATTATGCCTAAATTCATTCAAGCTATGGGTCAACAAATTGAACGCGACGCGGATCGTGAACGAAGTAGGATTTGGCTTCAAGAGGACATCCACACCTTGTTTAATGAACGACGTGTAAGACATATCTTTAGTATAAATGTTATTTTCCGATGATCTTTTTTGTTATTGAAGTTTTATTTTTGTGCTATATACTCAATTTTTCCAGGGATTAAAACACATCAAAGGCATAATTTTGGAGTTGGAAGAGAACCAAGATGAATTAGAGTTGGAAGGGAGGCTCTTTACAAATATGACATGTTTGAGGATGTTGAAAATTGGTAATGTGCAGCTTCGCAGAGACATCACAAATCTGTCCAGAGAATTGCGGTTGCTCCATTGGCATGGCTATCCTTTAGAGTGTTTGCCATCAGATTTTGAATCAAAATATTTATTTGAACTACTCCTACCTGTTGGTCAAACTATACAACTTTGGCAAGGGCGAAAGGTTAGTACATATAATTATCATTTCATTATGGGTATATTTGAGAGCGATGGTAGAATTAGTGATTTTGAAAAAAGAGATTTTAGTGTAATTGATTTTCTAAAATCATCATCAAAGACATATCTTAAAAATCAATTTAAAGTGGTTTTAAATTTTACTAAAATCAATTGGAAAGGAACGTGATTTTGATAATGGCAAAAGACAAAATCACTCACAAGCACCTCCTACGTTAGTTCGATTTTGTTTTGTTGCAGCTATAAACTTTGTCTCCTGTATGAAGTACTAATATCTCAAATAAATGTAAACAAACTGTGTTTTATTACAGAGATTTCAGAAACTGAAGGTCATCGATGCTAGTGATTCCAAGAATTTGTGCGAAACGCCAGATTTTTCTGAGGTTTCGAATCTTGAAAGATTGATTCTACGTAATTGTACAAGATTGCAGAAGATTGATTCTTCCATCAGCACCCTCAGTCGTCTAGTTTTATTGGATGTGAGGGGTTGTTTCAGTCTACAAAACTTTTCATTCTCACGTCTTCTAGACAATTTGGAGGTACTATTTGTTTGAACTTCGACATTTGTTTAGAAAGTAATCTAGACTTCGTTTTAAATTCATGAATTTTGTTCAATTACATGAACATTTTTTGTTGATACATTACAAGTTTCGTCCAAAATTCTTGTGAGTAAGAAGACAGACATCTTTTATAATAGTTTGTTATCCAATGTGAATCGCGATTTGATGAGATTATCAAACATACTCTCTGAAGTTAAACGATACGAGAACCTCAGTGAATTGGCTTAGAAACTCCTTGATTATGCGAACTTCCTTTGAATTTGATAGCTCATTATCAGTTGATCGAGAAGAAGAATGTGGGAGAGCAATGTATTATTTGGGGAGGAGAAAGTTACTTAGAATTGAGGGAGTTGGTGTTCACATATAATTTCCTCTTCCACAGAAATATCCAAATACAACTAAAAATATGTAAATAGGAAGAAAATTATAAAGAATAACGACACAGTAGATTATACTAATTCACCACTAAGGGGTCTTTCATCTCTAACTCACTCATAATTGTTCAAGGATTTGAATACAAAAACTTTCTCCAAAAATACCCCTAGAGGTCCCAACAAAACCCTCTATTTGAACACAATATGAATACTTAAGAACATGAATGAAAACTCACAAGAATCAAGATACAATAATTTCCCCGATAACGTAGTCTTGATCAACCCCAATTCCTCTGCCAATGTCCGAGCTGCCAAAAAACCTCTAAAAATGGCTGCTAAAAAACCCCTCTTTCGGTTGGTAAAACCCTAAACCCGAAGTGCAGTATTTATAAACATTGAAACAGCGTCATGGTGCTTTCCCTAGAGCCATGGCACTACGGACGGAGAGACACGCCCACGTGCCCCATCAGGACAGCATCGCGACGCTACCTCCTCGGCCCTTGTGGCCTACAGCGTTCCGCTTCCCTATTTTCATTCCCACTTCGATCCCAACACATCCCTAGTTCGTTCCGACGTCGTTTTGGTTACACCAATTCTAATAGTGGGGAGGGAATAA

mRNA sequence

ATGTTGTGTCCTAAATTAGTAGCTCCTGGCAAGTACAAGACTTTAGTTGAAATGTCACGCCGATTGGATAAAATGAATAAGCTCCTTGATTTTGATTTAGAATTGAATGATGTACGTTTTGTAGGAATAGTAGGGATGGGCGGTATTGGTAAGACAACTATTGCTGAAGTTGTTTATGACGACATTTTACTTGGATTTTGGAAGAACAGCTGCTTTCTTCGCATAACTCCGGAGCGCAATTTAGTCACTTTGCAACATCAGCTACTTTCCCAACTTCTTTTAGAAAAAGATATTGAAATTCGGGATGAGGATCAAGGAGCACAACTAATTAAGAATCGTTTGAGTGGTATAAAAGTTCTTATTGGTGCATTCTCACTCTTTTGCAAGCATGCATTTGGAGATGGTCGTCCTCTCGATGATAATTTAAAAGATCTTTGTAATGGAATTGTAAGACAAACTAGAAGACTTCCATTAGCTTTGATAAAGATTGGATCCTCTTTGTACGGTCAAAGTATAGACATATGGAGAGAAGAGCTTGAGAGTCTCACTAGAGCTCACAATAAACGCCTGTTTGACACATTAAAGATAAGTTTTGATGATATAGGAAAAACCAGTCAACAAGTTTTTCTTGATTTTGCTTGTTTCTTCAATGGAAAAGCAATAGATAGAGTGATTGAAATACTTAGGAGTTTAGGTTACATGCCGCACAGTGAGATGCAATTGTTGTGTGATAGATGTCTTATCGAAGTTGGACCCCAAAAAACAATAATTATGCCTAAATTCATTCAAGCTATGGGTCAACAAATTGAACGCGACGCGGATCGTGAACGAAGTAGGATTTGGCTTCAAGAGGACATCCACACCTTGTTTAATGAACGACGTGGATTAAAACACATCAAAGGCATAATTTTGGAGTTGGAAGAGAACCAAGATGAATTAGAGTTGGAAGGGAGGCTCTTTACAAATATGACATGTTTGAGGATGTTGAAAATTGGTAATGTGCAGCTTCGCAGAGACATCACAAATCTGTCCAGAGAATTGCGGTTGCTCCATTGGCATGGCTATCCTTTAGAGTGTTTGCCATCAGATTTTGAATCAAAATATTTATTTGAACTACTCCTACCTGTTGGTCAAACTATACAACTTTGGCAAGGGCGAAAGAGATTTCAGAAACTGAAGGTCATCGATGCTAGTGATTCCAAGAATTTGTGCGAAACGCCAGATTTTTCTGAGGTTTCGAATCTTGAAAGATTGATTCTACGTAATTGTACAAGATTGCAGAAGATTGATTCTTCCATCAGCACCCTCAGTCGTCTAGTTTTATTGGATGTGAGGGGTTGTTTCAGTCTACAAAACTTTTCATTCTCACGTCTTCTAGACAATTTGGAGAGCCATGGCACTACGGACGGAGAGACACGCCCACGTGCCCCATCAGGACAGCATCGCGACGCTACCTCCTCGGCCCTTGTGGCCTACAGCGTTCCGCTTCCCTATTTTCATTCCCACTTCGATCCCAACACATCCCTAGTTCGTTCCGACGTCGTTTTGGTTACACCAATTCTAATAGTGGGGAGGGAATAA

Coding sequence (CDS)

ATGTTGTGTCCTAAATTAGTAGCTCCTGGCAAGTACAAGACTTTAGTTGAAATGTCACGCCGATTGGATAAAATGAATAAGCTCCTTGATTTTGATTTAGAATTGAATGATGTACGTTTTGTAGGAATAGTAGGGATGGGCGGTATTGGTAAGACAACTATTGCTGAAGTTGTTTATGACGACATTTTACTTGGATTTTGGAAGAACAGCTGCTTTCTTCGCATAACTCCGGAGCGCAATTTAGTCACTTTGCAACATCAGCTACTTTCCCAACTTCTTTTAGAAAAAGATATTGAAATTCGGGATGAGGATCAAGGAGCACAACTAATTAAGAATCGTTTGAGTGGTATAAAAGTTCTTATTGGTGCATTCTCACTCTTTTGCAAGCATGCATTTGGAGATGGTCGTCCTCTCGATGATAATTTAAAAGATCTTTGTAATGGAATTGTAAGACAAACTAGAAGACTTCCATTAGCTTTGATAAAGATTGGATCCTCTTTGTACGGTCAAAGTATAGACATATGGAGAGAAGAGCTTGAGAGTCTCACTAGAGCTCACAATAAACGCCTGTTTGACACATTAAAGATAAGTTTTGATGATATAGGAAAAACCAGTCAACAAGTTTTTCTTGATTTTGCTTGTTTCTTCAATGGAAAAGCAATAGATAGAGTGATTGAAATACTTAGGAGTTTAGGTTACATGCCGCACAGTGAGATGCAATTGTTGTGTGATAGATGTCTTATCGAAGTTGGACCCCAAAAAACAATAATTATGCCTAAATTCATTCAAGCTATGGGTCAACAAATTGAACGCGACGCGGATCGTGAACGAAGTAGGATTTGGCTTCAAGAGGACATCCACACCTTGTTTAATGAACGACGTGGATTAAAACACATCAAAGGCATAATTTTGGAGTTGGAAGAGAACCAAGATGAATTAGAGTTGGAAGGGAGGCTCTTTACAAATATGACATGTTTGAGGATGTTGAAAATTGGTAATGTGCAGCTTCGCAGAGACATCACAAATCTGTCCAGAGAATTGCGGTTGCTCCATTGGCATGGCTATCCTTTAGAGTGTTTGCCATCAGATTTTGAATCAAAATATTTATTTGAACTACTCCTACCTGTTGGTCAAACTATACAACTTTGGCAAGGGCGAAAGAGATTTCAGAAACTGAAGGTCATCGATGCTAGTGATTCCAAGAATTTGTGCGAAACGCCAGATTTTTCTGAGGTTTCGAATCTTGAAAGATTGATTCTACGTAATTGTACAAGATTGCAGAAGATTGATTCTTCCATCAGCACCCTCAGTCGTCTAGTTTTATTGGATGTGAGGGGTTGTTTCAGTCTACAAAACTTTTCATTCTCACGTCTTCTAGACAATTTGGAGAGCCATGGCACTACGGACGGAGAGACACGCCCACGTGCCCCATCAGGACAGCATCGCGACGCTACCTCCTCGGCCCTTGTGGCCTACAGCGTTCCGCTTCCCTATTTTCATTCCCACTTCGATCCCAACACATCCCTAGTTCGTTCCGACGTCGTTTTGGTTACACCAATTCTAATAGTGGGGAGGGAATAA

Protein sequence

MLCPKLVAPGKYKTLVEMSRRLDKMNKLLDFDLELNDVRFVGIVGMGGIGKTTIAEVVYDDILLGFWKNSCFLRITPERNLVTLQHQLLSQLLLEKDIEIRDEDQGAQLIKNRLSGIKVLIGAFSLFCKHAFGDGRPLDDNLKDLCNGIVRQTRRLPLALIKIGSSLYGQSIDIWREELESLTRAHNKRLFDTLKISFDDIGKTSQQVFLDFACFFNGKAIDRVIEILRSLGYMPHSEMQLLCDRCLIEVGPQKTIIMPKFIQAMGQQIERDADRERSRIWLQEDIHTLFNERRGLKHIKGIILELEENQDELELEGRLFTNMTCLRMLKIGNVQLRRDITNLSRELRLLHWHGYPLECLPSDFESKYLFELLLPVGQTIQLWQGRKRFQKLKVIDASDSKNLCETPDFSEVSNLERLILRNCTRLQKIDSSISTLSRLVLLDVRGCFSLQNFSFSRLLDNLESHGTTDGETRPRAPSGQHRDATSSALVAYSVPLPYFHSHFDPNTSLVRSDVVLVTPILIVGRE
Homology
BLAST of MC11g_new0432 vs. ExPASy Swiss-Prot
Match: Q9SZ66 (Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=1 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 9.0e-53
Identity = 156/532 (29.32%), Postives = 246/532 (46.24%), Query Frame = 0

Query: 7   VAPGKYKTLVEMSRRLDKMNKLLDFDLELNDVRFVGIVGMGGIGKTTIAEVVYDDILLGF 66
           +AP   + LV +  RL  + KLL ++ +L+ V  +GIVGM GIGKTT+A+ +Y   + G 
Sbjct: 179 LAPSGNEGLVGIESRLKNLEKLLSWE-DLDTVHIIGIVGMVGIGKTTLADCLYGR-MRGQ 238

Query: 67  WKNSCFLRITPERN----LVTLQHQLLSQLLLEKDIEIRDEDQGAQLIKNRLSGIKVLI- 126
           +  SCFL    E +    L +L  +L S +L ++D+EI       +  + RL   ++LI 
Sbjct: 239 FDGSCFLTNIRENSGRSGLESLLQKLFSTVLNDRDLEIGAPGNAHERFERRLKSKRLLIV 298

Query: 127 ----------------------------------------------------GAFSLFCK 186
                                                                A  LF  
Sbjct: 299 LDDVNDEKQIRYLMGHCKWYQGGSRIIITTRDSKLIETIKGRKYVLPKLNDREALKLFSL 358

Query: 187 HAFGDGRPLDDNLKDLCNGIVRQTRRLPLALIKIGSSLYGQSIDIWREELESLTRAHNKR 246
           +AF +  PL +  + L N ++   +  PLAL  +GS L  +    W  +L+ L    +  
Sbjct: 359 NAFSNSFPLKE-FEGLTNMVLDYAKGHPLALKVLGSDLCERDDLYWEAKLDRLKSRSHGD 418

Query: 247 LFDTLKISFDDIGKTSQQVFLDFACFFNGKAIDRVIEILRSLGYMPHSEMQLLCDRCLIE 306
           +++ L+ S++++    + VFLD ACFF  + +D V  +L S G      ++ L D+CLI 
Sbjct: 419 IYEVLETSYEELTTEQKNVFLDIACFFRSENVDYVTSLLNSHGVDVSGVVKDLVDKCLIT 478

Query: 307 VGPQKTIIMPKFIQAMGQQIERDAD-----------------RERSRIWLQEDIHTLFNE 366
           +   + I M   +Q M ++I    +                 +   R+W  EDI  L  E
Sbjct: 479 LSDNR-IEMHDMLQTMAKEISLKVETIGIRDCRWLSRHGNQCQWHIRLWDSEDICDLLTE 538

Query: 367 RRGLKHIKGIILELEENQDELELEGRLFTNMTCLRMLKIGN------------VQLRRDI 426
             G   I+GI L+  + +  + L  + F  M  L+ LKI +            + LRR +
Sbjct: 539 GLGTDKIRGIFLDTSKLR-AMRLSAKAFQGMYNLKYLKIYDSHCSRGCEAEFKLHLRRGL 598

Query: 427 TNLSRELRLLHWHGYPLECLPSDFESKYLFELLLPVGQTIQLWQGRKRFQKLKVIDASDS 453
           + L  EL  LHWHGYPL+ +P DF+ K L +L LP  Q  ++W   K    LK +D S S
Sbjct: 599 SFLPNELTYLHWHGYPLQSIPLDFDPKNLVDLKLPHSQLEEIWDDEKDVGMLKWVDLSHS 658

BLAST of MC11g_new0432 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 3.2e-50
Identity = 148/504 (29.37%), Postives = 229/504 (45.44%), Query Frame = 0

Query: 13  KTLVEMSRRLDKMNKLLDFDLELNDVRFVGIVGMGGIGKTTIAEVVYDDILLGF-----W 72
           + +V +   L+K+  LL  ++ +N VR +GI GMGG+GKTTIA  ++D +L        +
Sbjct: 186 QNIVGIDTHLEKIESLL--EIGINGVRIMGIWGMGGVGKTTIARAIFDTLLGRMDSSYQF 245

Query: 73  KNSCFLRITPE--RNLVTLQHQLLSQLLLEKDIEIRDEDQGAQLIKNRLSGIKVLI---- 132
             +CFL+   E  R + +LQ+ LLS+LL EK     +E+ G   + +RL   KVLI    
Sbjct: 246 DGACFLKDIKENKRGMHSLQNALLSELLREK-ANYNNEEDGKHQMASRLRSKKVLIVLDD 305

Query: 133 -------------------------------------------------GAFSLFCKHAF 192
                                                             +  LF +HAF
Sbjct: 306 IDNKDHYLEYLAGDLDWFGNGSRIIITTRDKHLIEKNDIIYEVTALPDHESIQLFKQHAF 365

Query: 193 GDGRPLDDNLKDLCNGIVRQTRRLPLALIKIGSSLYGQSIDIWREELESLTRAHNKRLFD 252
           G   P ++N + L   +V   + LPLAL   GS L+   +  W+  +E +       + D
Sbjct: 366 GKEVP-NENFEKLSLEVVNYAKGLPLALKVWGSLLHNLRLTEWKSAIEHMKNNSYSGIID 425

Query: 253 TLKISFDDIGKTSQQVFLDFACFFNGKAIDRVIEILRSLGYMPHSEMQLLCDRCLIEVGP 312
            LKIS+D +    Q++FLD ACF  G+  D +++IL S        +++L D+ L+ +  
Sbjct: 426 KLKISYDGLEPKQQEMFLDIACFLRGEEKDYILQILESCHIGAEYGLRILIDKSLVFISE 485

Query: 313 QKTIIMPKFIQAMGQQI---ERDADRERSRIWLQEDIHTLFNERRGLKHIKGIILELEEN 372
              + M   IQ MG+ I   ++D   ERSR+WL +++  + +   G   ++ I   +   
Sbjct: 486 YNQVQMHDLIQDMGKYIVNFQKDPG-ERSRLWLAKEVEEVMSNNTGTMAMEAI--WVSSY 545

Query: 373 QDELELEGRLFTNMTCLRMLKIGNVQLRRDITNLSRELRLLHWHGYPLECLPSDFESKYL 432
              L    +   NM  LR+  +G       I  L   LR      YP E  PS FE K L
Sbjct: 546 SSTLRFSNQAVKNMKRLRVFNMGRSSTHYAIDYLPNNLRCFVCTNYPWESFPSTFELKML 605

Query: 433 FELLLPVGQTIQLWQGRKRFQKLKVIDASDSKNLCETPDFSEVSNLERLILRNCTRLQKI 454
             L L       LW   K    L+ ID S SK L  TPDF+ + NLE + L  C+ L+++
Sbjct: 606 VHLQLRHNSLRHLWTETKHLPSLRRIDLSWSKRLTRTPDFTGMPNLEYVNLYQCSNLEEV 665

BLAST of MC11g_new0432 vs. ExPASy Swiss-Prot
Match: V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 1.2e-49
Identity = 163/523 (31.17%), Postives = 234/523 (44.74%), Query Frame = 0

Query: 33  LELNDVRFVGIVGMGGIGKTTIAEVVYDDILLGFWKNSCFLRITPERN---LVTLQHQLL 92
           +E +DVR VG+ G+GGIGKTTIA+V+Y+++   F   S    I  + N   +  LQ+QLL
Sbjct: 217 MESSDVRMVGMYGVGGIGKTTIAKVIYNELSREFEYMSFLENIREKFNTQGVSPLQNQLL 276

Query: 93  SQLLL-EKDIEIRDEDQGAQLIKNRLS-----------------------------GIKV 152
             +L  E    I     GA +IK+ LS                             G +V
Sbjct: 277 DDILKGEGSQNINSVAHGASMIKDILSSKIVFIVLDDVDDQSQLEYLLRHREWLGEGSRV 336

Query: 153 LI-------------------------GAFSLFCKHAFGDGRPLDDNLKDLCNGIVRQTR 212
           +I                          A  LF  +AF    P  D  ++L + +V   +
Sbjct: 337 IITTRNKHVLDVQKVDDLYEVKGLNFEEACELFSLYAFEQNLPKSD-YRNLSHRVVGYCQ 396

Query: 213 RLPLALIKIGSSLYGQSIDIWREELESLTRAHNKRLFDTLKISFDDIGKTSQQVFLDFAC 272
            LPLAL  +G  L  ++I  W  EL  L R     +   LK S+D +G+T + +FLD AC
Sbjct: 397 GLPLALKVLGCLLLKKTIPEWESELRKLDREPEAEILSVLKRSYDGLGRTEKSIFLDVAC 456

Query: 273 FFNGKAIDRVIEILRSLGYMPHSEMQLLCDRCLIEVGPQKTIIMPKFIQAMGQQIER--- 332
           FF G+  D V +IL +  +     ++ L D+CLI +     I M   IQ MG +I R   
Sbjct: 457 FFKGEDRDFVSKILDACDFHAEIGIKNLNDKCLITL-QYNRIRMHDLIQQMGWEIVREKF 516

Query: 333 -DADRERSRIWLQEDIHTLFNERRGLKHIKGIILELEENQDELELEGRLFTNMTCLRMLK 392
            D   + SR+W   D        +G+K ++ I L+L + +  +      F  MT LR+LK
Sbjct: 517 PDEPNKWSRLWDTCDFERALTAYKGIKRVETISLDLSKLK-RVCSNSNAFAKMTRLRLLK 576

Query: 393 I-----------------------------GNVQLRRDITNLSRELRLLHWHGYPLECLP 452
           +                               ++L R     S ELR L W GYPL+ LP
Sbjct: 577 VQSSLDIDFEPEYIDADDKVELYDVVMKNASKMRLGRGFKFPSYELRYLRWDGYPLDFLP 636

Query: 453 SDFESKYLFELLLPVGQTIQLWQGRKRFQKLKVIDASDSKNLCETPDFSEVSNLERLILR 465
           S+F+   L EL L      QL  G K  + LKVID S S+ L +  +FS + NLERL LR
Sbjct: 637 SNFDGGKLVELHLKCSNIKQLRLGNKDLEMLKVIDLSYSRKLSQMSEFSSMPNLERLFLR 696

BLAST of MC11g_new0432 vs. ExPASy Swiss-Prot
Match: V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 1.6e-49
Identity = 164/527 (31.12%), Postives = 235/527 (44.59%), Query Frame = 0

Query: 28  LLDFDLELNDVRFVGIVGMGGIGKTTIAEVVYDDILLGFWKNSCFLRITPERN---LVTL 87
           +L   LE +DVR VGI G+GGIGKTTIA+V+Y+++   F   S    I    N   L  L
Sbjct: 207 ILRLHLESSDVRMVGIYGVGGIGKTTIAKVIYNELSCEFEYMSFLENIREGSNPQVLFHL 266

Query: 88  QHQLLSQLL----------------LEKDI-----------EIRDEDQGAQLIKNRL--- 147
           Q+QLL  +L                + KDI           ++ D  Q   L+ +R    
Sbjct: 267 QNQLLGDILEGEGSQNISSVAHRASMIKDILLSRRVFIVLDDVDDLSQLEYLLGHREWLG 326

Query: 148 SGIKVLI-------------------------GAFSLFCKHAFGDGRPLDDNLKDLCNGI 207
            G +V+I                          A  LF  +AF    P  D  ++L   +
Sbjct: 327 EGSRVIITTRNKHVLAVQEVDDLYEVEGLNFEEACELFSLYAFKQNLPKSD-YRNLTCRV 386

Query: 208 VRQTRRLPLALIKIGSSLYGQSIDIWREELESLTRAHNKRLFDTLKISFDDIGKTSQQVF 267
           V   + LPLAL  +GS L  ++I  W  EL+ L       +   LK S+D + +  + +F
Sbjct: 387 VGYCQGLPLALKVLGSLLCKKTIPQWEGELKKLDSEPKADIHKVLKRSYDGLDRIDKNIF 446

Query: 268 LDFACFFNGKAIDRVIEILRSLGYMPHSEMQLLCDRCLIEVGPQKTIIMPKFIQAMGQQI 327
           LD ACFF G+  D V+ IL    +   + +  L D CLI + P   I M   IQ MG +I
Sbjct: 447 LDLACFFKGEGRDFVLRILDGCDFPAETGISNLNDLCLITL-PYNQICMHDLIQQMGWEI 506

Query: 328 ERD----ADRERSRIWLQEDIHTLFNERRGLKHIKGIILELEENQDELELEGRLFTNMTC 387
            R+       + SR+W   D         G+K ++ + L+L + +  +     +F  MT 
Sbjct: 507 VRENFPVEPNKWSRLWDPCDFERALTADEGIKSVETMSLDLSKLK-RVCSNSNVFAKMTK 566

Query: 388 LRMLKI----------------------------GNVQLRRDITNLSRELRLLHWHGYPL 447
           LR+LK+                              +QL +     S ELR L W GYPL
Sbjct: 567 LRLLKVYSSSDIDSAHGDSDEDIEEVYDVVMKDASKMQLGQSFKFPSYELRYLRWDGYPL 626

Query: 448 ECLPSDFESKYLFELLLPVGQTIQLWQGRKRFQKLKVIDASDSKNLCETPDFSEVSNLER 465
           + LP +F+   L EL L      QLWQG K  ++LKVID S S+ L +  +FS + NLER
Sbjct: 627 DSLPLNFDGGKLVELHLKCSNIKQLWQGHKDLERLKVIDLSYSRKLSQMSEFSSMPNLER 686

BLAST of MC11g_new0432 vs. ExPASy Swiss-Prot
Match: F4JT80 (Disease resistance protein RPP2B OS=Arabidopsis thaliana OX=3702 GN=RPP2B PE=1 SV=2)

HSP 1 Score: 198.0 bits (502), Expect = 2.7e-49
Identity = 151/503 (30.02%), Postives = 232/503 (46.12%), Query Frame = 0

Query: 21  RLDKMNKLLDFDLELND--VRFVGIVGMGGIGKTTIAEVVYDDILLGFWKNSCFLRITPE 80
           R  ++ KLL FD   ND  VR VG++GM GIGKTT+A++VY      F     FL    +
Sbjct: 198 RSKELEKLLMFD---NDECVRVVGVLGMTGIGKTTVADIVYKQNFQRF-DGYEFLEDIED 257

Query: 81  RN----LVTLQHQLLSQLLLEKDIEIRDEDQGAQLIKNRL-----------SGIKVLIG- 140
            +    L  L  +LL +LL  +++++R + +    ++N+              I+ LIG 
Sbjct: 258 NSKRYGLPYLYQKLLHKLLDGENVDVRAQGRPENFLRNKKLFIVLDNVTEEKQIEYLIGK 317

Query: 141 -------------------------------------AFSLFCKHAFGDGRPLDDNLKDL 200
                                                A  LFC   FG+  P ++   DL
Sbjct: 318 KNVYRQGSRIVIITRDKKLLQKNADATYVVPRLNDREAMELFCLQVFGNHYPTEE-FVDL 377

Query: 201 CNGIVRQTRRLPLALIKIGSSLYGQSIDIWREELESLTRAHNKRLFDTLKISFDDIGKTS 260
            N  V   + LPLAL  +G  L    I+ W+++LE L    +K L   LK S+  +    
Sbjct: 378 SNDFVCYAKGLPLALKLLGKGLLTHDINYWKKKLEFLQVNPDKELQKELKSSYKALDDDQ 437

Query: 261 QQVFLDFACFFNGKAIDRVIEILRSLGYMPHSEMQLLCDRCLIEVGPQKTIIMPKFIQAM 320
           + VFLD ACFF  +  D V  IL+S        M+ L ++CL+ +   + I M   + AM
Sbjct: 438 KSVFLDIACFFRSEKADFVSSILKSDDIDAKDVMRELEEKCLVTISYDR-IEMHDLLHAM 497

Query: 321 GQQIERDAD----RERSRIWLQEDIHTLFNERRGLKHIKGIILELEENQDELELEGRLFT 380
           G++I ++       ER R+W  +DI  +     G + ++GI L + E +  ++L    FT
Sbjct: 498 GKEIGKEKSIRKAGERRRLWNHKDIRDILEHNTGTECVRGIFLNMSEVR-RIKLFPAAFT 557

Query: 381 NMTCLRMLKIGN------------VQLRRDITNLSRELRLLHWHGYPLECLPSDFESKYL 440
            ++ L+ LK  +             Q  +   +   EL  LHW GYP +CLPSDF+ K L
Sbjct: 558 MLSKLKFLKFHSSHCSQWCDNDHIFQCSKVPDHFPDELVYLHWQGYPYDCLPSDFDPKEL 617

Query: 441 FELLLPVGQTIQLWQGRKRFQKLKVIDASDSKNLCETPDFSEVSNLERLILRNCTRLQKI 453
            +L L      QLW+  K  + L+ +D   SK+L      S   NLERL L  CT L  +
Sbjct: 618 VDLSLRYSHIKQLWEDEKNTESLRWVDLGQSKDLLNLSGLSRAKNLERLDLEGCTSLDLL 677

BLAST of MC11g_new0432 vs. NCBI nr
Match: XP_022141771.1 (TMV resistance protein N-like isoform X2 [Momordica charantia])

HSP 1 Score: 511 bits (1315), Expect = 9.95e-171
Identity = 292/567 (51.50%), Postives = 363/567 (64.02%), Query Frame = 0

Query: 4   PKLVAPGKYKTLVEMSRRLDKMNKLLDFDLELNDVRFVGIVGMGGIGKTTIAEVVYDDIL 63
           PKLVA  K   LV++ RRL  M+ L+   LE NDV FVGI+GMGGIGKTTIAEV YD + 
Sbjct: 188 PKLVAADKLNNLVDIERRLYSMDTLIG--LESNDVCFVGIIGMGGIGKTTIAEVFYDKVA 247

Query: 64  LGFWKNSCFLRITPER-NLVTLQHQLLSQLLLEKDIEIRDEDQGAQLIKNRLSGIKVLI- 123
             F KN CFLRI     +L++LQHQLLSQLL ++DI+I DE++G  +IK  LSG KVLI 
Sbjct: 248 HKFGKNRCFLRIQGHSLSLISLQHQLLSQLLRKEDIKIMDENEGEYMIKYHLSGKKVLIV 307

Query: 124 --------------------------------------------------------GAFS 183
                                                                     FS
Sbjct: 308 LDGIDEKGQLEKLAGSPDWFGPKSQIIITTRNRDVLRQSNYKDKLLEYKVELLDYKSGFS 367

Query: 184 LFCKHAFGDGRPLDDNLKDLCNGIVRQTRRLPLALIKIGSSLYGQSIDIWREELESLTRA 243
           LFCKHAFGD    ++N + L   IV + +  P AL++IGS LYGQ ID+W+EEL SL   
Sbjct: 368 LFCKHAFGDHTCPNENFEQLSKEIVDRVKGHPQALVQIGSYLYGQDIDVWKEELNSLDID 427

Query: 244 HNKRLFDTLKISFDDIGKTSQQVFLDFACFFNGKAIDRVIEILRSLGYMPHSEMQLLCDR 303
           +NK +F TLKISFD + KTSQQVFLD ACFFNG+  D+VIEILRS  Y PHSE+QLL DR
Sbjct: 428 YNKHVFKTLKISFDGLEKTSQQVFLDLACFFNGRRKDKVIEILRSFDYRPHSEIQLLYDR 487

Query: 304 CLIEVGPQKTIIMPKFIQAMGQQIERDADRERSRIWLQEDIHTLFNERRGLKHIKGIILE 363
           CLIEV    TI +P  IQ MG++IERDAD ERSRIW+++D+  +F++R G+KHI+GI+L+
Sbjct: 488 CLIEVRRDGTIFIPNCIQTMGRKIERDAD-ERSRIWIRKDVLDVFDKRHGVKHIEGIVLD 547

Query: 364 LEENQDELELEGRLFTNMTCLRMLKIGNVQLRRDITNLSRELRLLHWHGYPLECLPSDFE 423
           LEE QD+L+L+  LF +MT LR+L+IGNV+   D T+LS++LRLL+WHGYP  CLP  FE
Sbjct: 548 LEEKQDKLKLQAGLFEDMTGLRILEIGNVEFCEDFTHLSKQLRLLNWHGYPSTCLPLQFE 607

Query: 424 SKYLFELLLPVGQTIQLWQGRKRFQKLKVIDASDSKNLCETPDFSEVSNLERLILRNCTR 483
           S+YLFELLLPVGQ  QLW+G+K F+KLKVID SDS+NL ETP+F++V NLE L+LRNCTR
Sbjct: 608 SRYLFELLLPVGQNTQLWKGQKGFEKLKVIDVSDSRNLRETPNFTKVPNLESLVLRNCTR 667

Query: 484 LQKIDSSISTLSRLVLLDVRGCFSLQNFSFSRLLDNLESHGTTDGETRPRAPSGQHR--- 501
           L  ID SIS L+RL  LD+  C SL++FS      +L         T   A SG  R   
Sbjct: 668 LYMIDHSISRLNRLTSLDLTCCVSLRSFSVINSCKSLT--------TLKLAGSGLERLKG 727

BLAST of MC11g_new0432 vs. NCBI nr
Match: XP_022141770.1 (TMV resistance protein N-like isoform X1 [Momordica charantia])

HSP 1 Score: 509 bits (1312), Expect = 5.16e-170
Identity = 277/509 (54.42%), Postives = 345/509 (67.78%), Query Frame = 0

Query: 4   PKLVAPGKYKTLVEMSRRLDKMNKLLDFDLELNDVRFVGIVGMGGIGKTTIAEVVYDDIL 63
           PKLVA  K   LV++ RRL  M+ L+   LE NDV FVGI+GMGGIGKTTIAEV YD + 
Sbjct: 188 PKLVAADKLNNLVDIERRLYSMDTLIG--LESNDVCFVGIIGMGGIGKTTIAEVFYDKVA 247

Query: 64  LGFWKNSCFLRITPER-NLVTLQHQLLSQLLLEKDIEIRDEDQGAQLIKNRLSGIKVLI- 123
             F KN CFLRI     +L++LQHQLLSQLL ++DI+I DE++G  +IK  LSG KVLI 
Sbjct: 248 HKFGKNRCFLRIQGHSLSLISLQHQLLSQLLRKEDIKIMDENEGEYMIKYHLSGKKVLIV 307

Query: 124 --------------------------------------------------------GAFS 183
                                                                     FS
Sbjct: 308 LDGIDEKGQLEKLAGSPDWFGPKSQIIITTRNRDVLRQSNYKDKLLEYKVELLDYKSGFS 367

Query: 184 LFCKHAFGDGRPLDDNLKDLCNGIVRQTRRLPLALIKIGSSLYGQSIDIWREELESLTRA 243
           LFCKHAFGD    ++N + L   IV + +  P AL++IGS LYGQ ID+W+EEL SL   
Sbjct: 368 LFCKHAFGDHTCPNENFEQLSKEIVDRVKGHPQALVQIGSYLYGQDIDVWKEELNSLDID 427

Query: 244 HNKRLFDTLKISFDDIGKTSQQVFLDFACFFNGKAIDRVIEILRSLGYMPHSEMQLLCDR 303
           +NK +F TLKISFD + KTSQQVFLD ACFFNG+  D+VIEILRS  Y PHSE+QLL DR
Sbjct: 428 YNKHVFKTLKISFDGLEKTSQQVFLDLACFFNGRRKDKVIEILRSFDYRPHSEIQLLYDR 487

Query: 304 CLIEVGPQKTIIMPKFIQAMGQQIERDADRERSRIWLQEDIHTLFNERRGLKHIKGIILE 363
           CLIEV    TI +P  IQ MG++IERDAD ERSRIW+++D+  +F++R G+KHI+GI+L+
Sbjct: 488 CLIEVRRDGTIFIPNCIQTMGRKIERDAD-ERSRIWIRKDVLDVFDKRHGVKHIEGIVLD 547

Query: 364 LEENQDELELEGRLFTNMTCLRMLKIGNVQLRRDITNLSRELRLLHWHGYPLECLPSDFE 423
           LEE QD+L+L+  LF +MT LR+L+IGNV+   D T+LS++LRLL+WHGYP  CLP  FE
Sbjct: 548 LEEKQDKLKLQAGLFEDMTGLRILEIGNVEFCEDFTHLSKQLRLLNWHGYPSTCLPLQFE 607

Query: 424 SKYLFELLLPVGQTIQLWQGRKRFQKLKVIDASDSKNLCETPDFSEVSNLERLILRNCTR 454
           S+YLFELLLPVGQ  QLW+G+K F+KLKVID SDS+NL ETP+F++V NLE L+LRNCTR
Sbjct: 608 SRYLFELLLPVGQNTQLWKGQKGFEKLKVIDVSDSRNLRETPNFTKVPNLESLVLRNCTR 667

BLAST of MC11g_new0432 vs. NCBI nr
Match: XP_038891076.1 (disease resistance protein RPV1-like isoform X3 [Benincasa hispida] >XP_038891077.1 disease resistance protein RPV1-like isoform X3 [Benincasa hispida])

HSP 1 Score: 498 bits (1283), Expect = 4.00e-166
Identity = 276/505 (54.65%), Postives = 345/505 (68.32%), Query Frame = 0

Query: 6   LVAPGKYKTLVEMSRRLDKMNKLLDFDLELNDVRFVGIVGMGGIGKTTIAEVVYDDILLG 65
           LVA  K   LV+M RRL KM++L+D  LE NDVRFVGI GMGGIGKTTIAEV YD +   
Sbjct: 181 LVASDKVDRLVDMERRLSKMDELID--LESNDVRFVGITGMGGIGKTTIAEVFYDKVANK 240

Query: 66  FWKNSCFLRITPERNLVTLQHQLLSQLLLEKDIEIRDEDQGAQLIKNRLSGIKVLIG--- 125
           F KN CFLRI  ER LV+LQ QLLSQLL  KDI I +E++GA++I++ L G KV I    
Sbjct: 241 FGKNHCFLRIY-ERTLVSLQQQLLSQLLRTKDIVINNENEGARMIESYLKGKKVFIALDG 300

Query: 126 ------------------------------------------------------AFSLFC 185
                                                                 A SLFC
Sbjct: 301 VKEISQLEMLVGSPIWFGQGSKIIITTRNRDVLRQPNYRDIMVEYNVEFLDYESAMSLFC 360

Query: 186 KHAFGDGRPLDDNLKDLCNGIVRQTRRLPLALIKIGSSLYGQSIDIWREELESLTRAH-N 245
           KHAFG   P + N +DL   IV + +  P ALI+IG SLY + IDIW+EEL+SL + + N
Sbjct: 361 KHAFGSEFP-NRNFEDLSKEIVERVKGHPQALIQIGLSLYDKGIDIWKEELKSLEKDYMN 420

Query: 246 KRLFDTLKISFDDIGKTSQQVFLDFACFFNGKAIDRVIEILRSLGYMPHSEMQLLCDRCL 305
           K LF TLKISFDD+GKTSQ+VFLD ACFFNGK  ++VIEIL+S  Y PHSE++LL DRCL
Sbjct: 421 KHLFKTLKISFDDLGKTSQEVFLDLACFFNGKKKEKVIEILKSFDYRPHSELKLLEDRCL 480

Query: 306 IEVGPQKTIIMPKFIQAMGQQIERDADRERSRIWLQEDIHTLFNERRGLKHIKGIILELE 365
           IEV    TI MP  IQAMGQ+IER AD+ RSRIWL +D    F++R  ++ IKGI+LE+E
Sbjct: 481 IEVRRDNTIFMPNCIQAMGQEIERGADK-RSRIWLPKDAIDAFDQRHRVRDIKGIVLEME 540

Query: 366 ENQDELELEGRLFTNMTCLRMLKIGNVQLRRDITNLSRELRLLHWHGYPLECLPSDFESK 425
           E QDE+ELEG++F +MTCL++L+I NV++  D T+LS++LRLL+WH YP ECLP  F+S+
Sbjct: 541 EKQDEVELEGKVFEDMTCLKILQISNVKVIGDFTHLSKQLRLLNWHSYPSECLPLRFDSR 600

Query: 426 YLFELLLPVGQTIQLWQGRKRFQKLKVIDASDSKNLCETPDFSEVSNLERLILRNCTRLQ 452
           YLF+LLLP+ QT QLW G+K F+KLK+I+ SDSKNL ETP+F++V NLE L+L NCTRL 
Sbjct: 601 YLFQLLLPLSQTRQLWNGQKGFEKLKLINVSDSKNLRETPNFTKVPNLESLVLSNCTRLW 660

BLAST of MC11g_new0432 vs. NCBI nr
Match: XP_038891073.1 (disease resistance protein RPV1-like isoform X2 [Benincasa hispida] >XP_038891074.1 disease resistance protein RPV1-like isoform X2 [Benincasa hispida] >XP_038891075.1 disease resistance protein RPV1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 498 bits (1283), Expect = 4.25e-166
Identity = 276/505 (54.65%), Postives = 345/505 (68.32%), Query Frame = 0

Query: 6   LVAPGKYKTLVEMSRRLDKMNKLLDFDLELNDVRFVGIVGMGGIGKTTIAEVVYDDILLG 65
           LVA  K   LV+M RRL KM++L+D  LE NDVRFVGI GMGGIGKTTIAEV YD +   
Sbjct: 181 LVASDKVDRLVDMERRLSKMDELID--LESNDVRFVGITGMGGIGKTTIAEVFYDKVANK 240

Query: 66  FWKNSCFLRITPERNLVTLQHQLLSQLLLEKDIEIRDEDQGAQLIKNRLSGIKVLIG--- 125
           F KN CFLRI  ER LV+LQ QLLSQLL  KDI I +E++GA++I++ L G KV I    
Sbjct: 241 FGKNHCFLRIY-ERTLVSLQQQLLSQLLRTKDIVINNENEGARMIESYLKGKKVFIALDG 300

Query: 126 ------------------------------------------------------AFSLFC 185
                                                                 A SLFC
Sbjct: 301 VKEISQLEMLVGSPIWFGQGSKIIITTRNRDVLRQPNYRDIMVEYNVEFLDYESAMSLFC 360

Query: 186 KHAFGDGRPLDDNLKDLCNGIVRQTRRLPLALIKIGSSLYGQSIDIWREELESLTRAH-N 245
           KHAFG   P + N +DL   IV + +  P ALI+IG SLY + IDIW+EEL+SL + + N
Sbjct: 361 KHAFGSEFP-NRNFEDLSKEIVERVKGHPQALIQIGLSLYDKGIDIWKEELKSLEKDYMN 420

Query: 246 KRLFDTLKISFDDIGKTSQQVFLDFACFFNGKAIDRVIEILRSLGYMPHSEMQLLCDRCL 305
           K LF TLKISFDD+GKTSQ+VFLD ACFFNGK  ++VIEIL+S  Y PHSE++LL DRCL
Sbjct: 421 KHLFKTLKISFDDLGKTSQEVFLDLACFFNGKKKEKVIEILKSFDYRPHSELKLLEDRCL 480

Query: 306 IEVGPQKTIIMPKFIQAMGQQIERDADRERSRIWLQEDIHTLFNERRGLKHIKGIILELE 365
           IEV    TI MP  IQAMGQ+IER AD+ RSRIWL +D    F++R  ++ IKGI+LE+E
Sbjct: 481 IEVRRDNTIFMPNCIQAMGQEIERGADK-RSRIWLPKDAIDAFDQRHRVRDIKGIVLEME 540

Query: 366 ENQDELELEGRLFTNMTCLRMLKIGNVQLRRDITNLSRELRLLHWHGYPLECLPSDFESK 425
           E QDE+ELEG++F +MTCL++L+I NV++  D T+LS++LRLL+WH YP ECLP  F+S+
Sbjct: 541 EKQDEVELEGKVFEDMTCLKILQISNVKVIGDFTHLSKQLRLLNWHSYPSECLPLRFDSR 600

Query: 426 YLFELLLPVGQTIQLWQGRKRFQKLKVIDASDSKNLCETPDFSEVSNLERLILRNCTRLQ 452
           YLF+LLLP+ QT QLW G+K F+KLK+I+ SDSKNL ETP+F++V NLE L+L NCTRL 
Sbjct: 601 YLFQLLLPLSQTRQLWNGQKGFEKLKLINVSDSKNLRETPNFTKVPNLESLVLSNCTRLW 660

BLAST of MC11g_new0432 vs. NCBI nr
Match: XP_038891070.1 (disease resistance protein RPV1-like isoform X1 [Benincasa hispida] >XP_038891071.1 disease resistance protein RPV1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 498 bits (1283), Expect = 5.94e-166
Identity = 276/505 (54.65%), Postives = 345/505 (68.32%), Query Frame = 0

Query: 6   LVAPGKYKTLVEMSRRLDKMNKLLDFDLELNDVRFVGIVGMGGIGKTTIAEVVYDDILLG 65
           LVA  K   LV+M RRL KM++L+D  LE NDVRFVGI GMGGIGKTTIAEV YD +   
Sbjct: 181 LVASDKVDRLVDMERRLSKMDELID--LESNDVRFVGITGMGGIGKTTIAEVFYDKVANK 240

Query: 66  FWKNSCFLRITPERNLVTLQHQLLSQLLLEKDIEIRDEDQGAQLIKNRLSGIKVLIG--- 125
           F KN CFLRI  ER LV+LQ QLLSQLL  KDI I +E++GA++I++ L G KV I    
Sbjct: 241 FGKNHCFLRIY-ERTLVSLQQQLLSQLLRTKDIVINNENEGARMIESYLKGKKVFIALDG 300

Query: 126 ------------------------------------------------------AFSLFC 185
                                                                 A SLFC
Sbjct: 301 VKEISQLEMLVGSPIWFGQGSKIIITTRNRDVLRQPNYRDIMVEYNVEFLDYESAMSLFC 360

Query: 186 KHAFGDGRPLDDNLKDLCNGIVRQTRRLPLALIKIGSSLYGQSIDIWREELESLTRAH-N 245
           KHAFG   P + N +DL   IV + +  P ALI+IG SLY + IDIW+EEL+SL + + N
Sbjct: 361 KHAFGSEFP-NRNFEDLSKEIVERVKGHPQALIQIGLSLYDKGIDIWKEELKSLEKDYMN 420

Query: 246 KRLFDTLKISFDDIGKTSQQVFLDFACFFNGKAIDRVIEILRSLGYMPHSEMQLLCDRCL 305
           K LF TLKISFDD+GKTSQ+VFLD ACFFNGK  ++VIEIL+S  Y PHSE++LL DRCL
Sbjct: 421 KHLFKTLKISFDDLGKTSQEVFLDLACFFNGKKKEKVIEILKSFDYRPHSELKLLEDRCL 480

Query: 306 IEVGPQKTIIMPKFIQAMGQQIERDADRERSRIWLQEDIHTLFNERRGLKHIKGIILELE 365
           IEV    TI MP  IQAMGQ+IER AD+ RSRIWL +D    F++R  ++ IKGI+LE+E
Sbjct: 481 IEVRRDNTIFMPNCIQAMGQEIERGADK-RSRIWLPKDAIDAFDQRHRVRDIKGIVLEME 540

Query: 366 ENQDELELEGRLFTNMTCLRMLKIGNVQLRRDITNLSRELRLLHWHGYPLECLPSDFESK 425
           E QDE+ELEG++F +MTCL++L+I NV++  D T+LS++LRLL+WH YP ECLP  F+S+
Sbjct: 541 EKQDEVELEGKVFEDMTCLKILQISNVKVIGDFTHLSKQLRLLNWHSYPSECLPLRFDSR 600

Query: 426 YLFELLLPVGQTIQLWQGRKRFQKLKVIDASDSKNLCETPDFSEVSNLERLILRNCTRLQ 452
           YLF+LLLP+ QT QLW G+K F+KLK+I+ SDSKNL ETP+F++V NLE L+L NCTRL 
Sbjct: 601 YLFQLLLPLSQTRQLWNGQKGFEKLKLINVSDSKNLRETPNFTKVPNLESLVLSNCTRLW 660

BLAST of MC11g_new0432 vs. ExPASy TrEMBL
Match: A0A6J1CK85 (TMV resistance protein N-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111012052 PE=4 SV=1)

HSP 1 Score: 511 bits (1315), Expect = 4.82e-171
Identity = 292/567 (51.50%), Postives = 363/567 (64.02%), Query Frame = 0

Query: 4   PKLVAPGKYKTLVEMSRRLDKMNKLLDFDLELNDVRFVGIVGMGGIGKTTIAEVVYDDIL 63
           PKLVA  K   LV++ RRL  M+ L+   LE NDV FVGI+GMGGIGKTTIAEV YD + 
Sbjct: 188 PKLVAADKLNNLVDIERRLYSMDTLIG--LESNDVCFVGIIGMGGIGKTTIAEVFYDKVA 247

Query: 64  LGFWKNSCFLRITPER-NLVTLQHQLLSQLLLEKDIEIRDEDQGAQLIKNRLSGIKVLI- 123
             F KN CFLRI     +L++LQHQLLSQLL ++DI+I DE++G  +IK  LSG KVLI 
Sbjct: 248 HKFGKNRCFLRIQGHSLSLISLQHQLLSQLLRKEDIKIMDENEGEYMIKYHLSGKKVLIV 307

Query: 124 --------------------------------------------------------GAFS 183
                                                                     FS
Sbjct: 308 LDGIDEKGQLEKLAGSPDWFGPKSQIIITTRNRDVLRQSNYKDKLLEYKVELLDYKSGFS 367

Query: 184 LFCKHAFGDGRPLDDNLKDLCNGIVRQTRRLPLALIKIGSSLYGQSIDIWREELESLTRA 243
           LFCKHAFGD    ++N + L   IV + +  P AL++IGS LYGQ ID+W+EEL SL   
Sbjct: 368 LFCKHAFGDHTCPNENFEQLSKEIVDRVKGHPQALVQIGSYLYGQDIDVWKEELNSLDID 427

Query: 244 HNKRLFDTLKISFDDIGKTSQQVFLDFACFFNGKAIDRVIEILRSLGYMPHSEMQLLCDR 303
           +NK +F TLKISFD + KTSQQVFLD ACFFNG+  D+VIEILRS  Y PHSE+QLL DR
Sbjct: 428 YNKHVFKTLKISFDGLEKTSQQVFLDLACFFNGRRKDKVIEILRSFDYRPHSEIQLLYDR 487

Query: 304 CLIEVGPQKTIIMPKFIQAMGQQIERDADRERSRIWLQEDIHTLFNERRGLKHIKGIILE 363
           CLIEV    TI +P  IQ MG++IERDAD ERSRIW+++D+  +F++R G+KHI+GI+L+
Sbjct: 488 CLIEVRRDGTIFIPNCIQTMGRKIERDAD-ERSRIWIRKDVLDVFDKRHGVKHIEGIVLD 547

Query: 364 LEENQDELELEGRLFTNMTCLRMLKIGNVQLRRDITNLSRELRLLHWHGYPLECLPSDFE 423
           LEE QD+L+L+  LF +MT LR+L+IGNV+   D T+LS++LRLL+WHGYP  CLP  FE
Sbjct: 548 LEEKQDKLKLQAGLFEDMTGLRILEIGNVEFCEDFTHLSKQLRLLNWHGYPSTCLPLQFE 607

Query: 424 SKYLFELLLPVGQTIQLWQGRKRFQKLKVIDASDSKNLCETPDFSEVSNLERLILRNCTR 483
           S+YLFELLLPVGQ  QLW+G+K F+KLKVID SDS+NL ETP+F++V NLE L+LRNCTR
Sbjct: 608 SRYLFELLLPVGQNTQLWKGQKGFEKLKVIDVSDSRNLRETPNFTKVPNLESLVLRNCTR 667

Query: 484 LQKIDSSISTLSRLVLLDVRGCFSLQNFSFSRLLDNLESHGTTDGETRPRAPSGQHR--- 501
           L  ID SIS L+RL  LD+  C SL++FS      +L         T   A SG  R   
Sbjct: 668 LYMIDHSISRLNRLTSLDLTCCVSLRSFSVINSCKSLT--------TLKLAGSGLERLKG 727

BLAST of MC11g_new0432 vs. ExPASy TrEMBL
Match: A0A6J1CJ23 (TMV resistance protein N-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012052 PE=4 SV=1)

HSP 1 Score: 509 bits (1312), Expect = 2.50e-170
Identity = 277/509 (54.42%), Postives = 345/509 (67.78%), Query Frame = 0

Query: 4   PKLVAPGKYKTLVEMSRRLDKMNKLLDFDLELNDVRFVGIVGMGGIGKTTIAEVVYDDIL 63
           PKLVA  K   LV++ RRL  M+ L+   LE NDV FVGI+GMGGIGKTTIAEV YD + 
Sbjct: 188 PKLVAADKLNNLVDIERRLYSMDTLIG--LESNDVCFVGIIGMGGIGKTTIAEVFYDKVA 247

Query: 64  LGFWKNSCFLRITPER-NLVTLQHQLLSQLLLEKDIEIRDEDQGAQLIKNRLSGIKVLI- 123
             F KN CFLRI     +L++LQHQLLSQLL ++DI+I DE++G  +IK  LSG KVLI 
Sbjct: 248 HKFGKNRCFLRIQGHSLSLISLQHQLLSQLLRKEDIKIMDENEGEYMIKYHLSGKKVLIV 307

Query: 124 --------------------------------------------------------GAFS 183
                                                                     FS
Sbjct: 308 LDGIDEKGQLEKLAGSPDWFGPKSQIIITTRNRDVLRQSNYKDKLLEYKVELLDYKSGFS 367

Query: 184 LFCKHAFGDGRPLDDNLKDLCNGIVRQTRRLPLALIKIGSSLYGQSIDIWREELESLTRA 243
           LFCKHAFGD    ++N + L   IV + +  P AL++IGS LYGQ ID+W+EEL SL   
Sbjct: 368 LFCKHAFGDHTCPNENFEQLSKEIVDRVKGHPQALVQIGSYLYGQDIDVWKEELNSLDID 427

Query: 244 HNKRLFDTLKISFDDIGKTSQQVFLDFACFFNGKAIDRVIEILRSLGYMPHSEMQLLCDR 303
           +NK +F TLKISFD + KTSQQVFLD ACFFNG+  D+VIEILRS  Y PHSE+QLL DR
Sbjct: 428 YNKHVFKTLKISFDGLEKTSQQVFLDLACFFNGRRKDKVIEILRSFDYRPHSEIQLLYDR 487

Query: 304 CLIEVGPQKTIIMPKFIQAMGQQIERDADRERSRIWLQEDIHTLFNERRGLKHIKGIILE 363
           CLIEV    TI +P  IQ MG++IERDAD ERSRIW+++D+  +F++R G+KHI+GI+L+
Sbjct: 488 CLIEVRRDGTIFIPNCIQTMGRKIERDAD-ERSRIWIRKDVLDVFDKRHGVKHIEGIVLD 547

Query: 364 LEENQDELELEGRLFTNMTCLRMLKIGNVQLRRDITNLSRELRLLHWHGYPLECLPSDFE 423
           LEE QD+L+L+  LF +MT LR+L+IGNV+   D T+LS++LRLL+WHGYP  CLP  FE
Sbjct: 548 LEEKQDKLKLQAGLFEDMTGLRILEIGNVEFCEDFTHLSKQLRLLNWHGYPSTCLPLQFE 607

Query: 424 SKYLFELLLPVGQTIQLWQGRKRFQKLKVIDASDSKNLCETPDFSEVSNLERLILRNCTR 454
           S+YLFELLLPVGQ  QLW+G+K F+KLKVID SDS+NL ETP+F++V NLE L+LRNCTR
Sbjct: 608 SRYLFELLLPVGQNTQLWKGQKGFEKLKVIDVSDSRNLRETPNFTKVPNLESLVLRNCTR 667

BLAST of MC11g_new0432 vs. ExPASy TrEMBL
Match: Q5DMW2 (MRGH9 OS=Cucumis melo OX=3656 GN=MRGH9 PE=4 SV=1)

HSP 1 Score: 491 bits (1265), Expect = 5.55e-164
Identity = 271/515 (52.62%), Postives = 344/515 (66.80%), Query Frame = 0

Query: 5   KLVAPGKYKTLVEMSRRLDKMNKLLDFDLELNDVRFVGIVGMGGIGKTTIAEVVYDDILL 64
           KLV   +   LV++ R+L KM KL D  LE N VRF+GI+GMGGIGKTTIAEV YD +  
Sbjct: 182 KLVPWDELTKLVDIERQLFKMEKLND--LEPNVVRFIGIIGMGGIGKTTIAEVFYDRVAR 241

Query: 65  GFWKNSCFLRITPERNLVTLQHQLLSQLLLEKDIEIRDEDQGAQLIKNRL---------- 124
            F KN CFLRI     L++LQ QLLSQLL  KD+ I +E++GA++I +RL          
Sbjct: 242 IFGKNRCFLRIYEHTTLLSLQQQLLSQLLQTKDLIINNENEGARMIGSRLKDKRVLIVLD 301

Query: 125 -------------------SGIKVLI----------------------------GAFSLF 184
                              SG K++I                             A +LF
Sbjct: 302 GVKEKSQLDQLVGNPNWFGSGSKIIITTRNRDVLRQPNYKDKMVEYSMEFLDTKSAMTLF 361

Query: 185 CKHAFGDGRPLDDNLKDLCNGIVRQTRRLPLALIKIGSSLYGQSIDIWREELESLTRAHN 244
           CKHAFG G P   N +D    IV + +  P ALI+IGSSLY + I+IW+EEL+SL   +N
Sbjct: 362 CKHAFGCGFP-SKNFEDFSKEIVERVKGHPQALIQIGSSLYDKGIEIWKEELKSLEEDYN 421

Query: 245 KRLFDTLKISFDDIGKTSQQVFLDFACFFNGKAIDRVIEILRSLGYMPHSEMQLLCDRCL 304
            R+F TLKISFDD+ KTSQ+VFLD ACFFN K  ++VIEIL+S  Y PHSE+QLL DRCL
Sbjct: 422 NRIFKTLKISFDDLEKTSQEVFLDLACFFNEKTKEKVIEILKSFDYRPHSEIQLLQDRCL 481

Query: 305 IEVGPQKTIIMPKFIQAMGQQIERDADRERSRIWLQEDIHTLFNERRGLKHIKGIILELE 364
           IEV    TI MPK IQ MGQQIER+AD+ RSRIW+ +D   +F+E   +K IKG++L+LE
Sbjct: 482 IEVRSDNTIFMPKCIQTMGQQIEREADK-RSRIWIPKDAQDVFDEPHRVKDIKGVVLKLE 541

Query: 365 ENQDELELEGRLFTNMTCLRMLKIGNVQLRRDITNLSRELRLLHWHGYPLECLPSDFESK 424
           E QDE+ELEG++F +M  L++L+IGNV++  D T+LS++LRLL+WH YP +CLP  FES+
Sbjct: 542 EKQDEIELEGKVFEDMRSLKILEIGNVEVSGDFTHLSKQLRLLNWHSYPSQCLPLSFESR 601

Query: 425 YLFELLLPVGQTIQLWQGRKRFQKLKVIDASDSKNLCETPDFSEVSNLERLILRNCTRLQ 462
           YLF+LLLP+ QT QLW G+K F+KLKVI+ S SKNL ETP+F++V NLE L L NCTRL 
Sbjct: 602 YLFQLLLPLSQTRQLWNGQKGFEKLKVINVSGSKNLRETPNFTKVPNLESLDLSNCTRLW 661

BLAST of MC11g_new0432 vs. ExPASy TrEMBL
Match: A0A1S3C9Z0 (TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103498649 PE=4 SV=1)

HSP 1 Score: 491 bits (1265), Expect = 2.04e-163
Identity = 271/515 (52.62%), Postives = 344/515 (66.80%), Query Frame = 0

Query: 5   KLVAPGKYKTLVEMSRRLDKMNKLLDFDLELNDVRFVGIVGMGGIGKTTIAEVVYDDILL 64
           KLV   +   LV++ R+L KM KL D  LE N VRF+GI+GMGGIGKTTIAEV YD +  
Sbjct: 182 KLVPWDELTKLVDIERQLFKMEKLND--LEPNVVRFIGIIGMGGIGKTTIAEVFYDRVAR 241

Query: 65  GFWKNSCFLRITPERNLVTLQHQLLSQLLLEKDIEIRDEDQGAQLIKNRL---------- 124
            F KN CFLRI     L++LQ QLLSQLL  KD+ I +E++GA++I +RL          
Sbjct: 242 IFGKNRCFLRIYEHTTLLSLQQQLLSQLLQTKDLIINNENEGARMIGSRLKDKRVLIVLD 301

Query: 125 -------------------SGIKVLI----------------------------GAFSLF 184
                              SG K++I                             A +LF
Sbjct: 302 GVKEKSQLEQLVGNPNWFGSGSKIIITTRNRDVLRQPNYKDKMVEYSMEFLDTKSAMTLF 361

Query: 185 CKHAFGDGRPLDDNLKDLCNGIVRQTRRLPLALIKIGSSLYGQSIDIWREELESLTRAHN 244
           CKHAFG G P   N +D    IV + +  P ALI+IGSSLY + I+IW+EEL+SL   +N
Sbjct: 362 CKHAFGCGFP-SKNFEDFSKEIVERVKGHPQALIQIGSSLYDKGIEIWKEELKSLEEDYN 421

Query: 245 KRLFDTLKISFDDIGKTSQQVFLDFACFFNGKAIDRVIEILRSLGYMPHSEMQLLCDRCL 304
            R+F TLKISFDD+ KTSQ+VFLD ACFFN K  ++VIEIL+S  Y PHSE+QLL DRCL
Sbjct: 422 NRIFKTLKISFDDLEKTSQEVFLDLACFFNEKTKEKVIEILKSFDYRPHSEIQLLQDRCL 481

Query: 305 IEVGPQKTIIMPKFIQAMGQQIERDADRERSRIWLQEDIHTLFNERRGLKHIKGIILELE 364
           IEV    TI MPK IQ MGQQIER+AD+ RSRIW+ +D   +F+E   +K IKG++L+LE
Sbjct: 482 IEVRSDNTIFMPKCIQTMGQQIEREADK-RSRIWIPKDAQDVFDEPHRVKDIKGVVLKLE 541

Query: 365 ENQDELELEGRLFTNMTCLRMLKIGNVQLRRDITNLSRELRLLHWHGYPLECLPSDFESK 424
           E QDE+ELEG++F +M  L++L+IGNV++  D T+LS++LRLL+WH YP +CLP  FES+
Sbjct: 542 EKQDEIELEGKVFEDMRSLKILEIGNVEVSGDFTHLSKQLRLLNWHSYPSQCLPLSFESR 601

Query: 425 YLFELLLPVGQTIQLWQGRKRFQKLKVIDASDSKNLCETPDFSEVSNLERLILRNCTRLQ 462
           YLF+LLLP+ QT QLW G+K F+KLKVI+ S SKNL ETP+F++V NLE L L NCTRL 
Sbjct: 602 YLFQLLLPLSQTRQLWNGQKGFEKLKVINVSGSKNLRETPNFTKVPNLESLDLSNCTRLW 661

BLAST of MC11g_new0432 vs. ExPASy TrEMBL
Match: M4QSV9 (Fom-1 OS=Cucumis melo OX=3656 GN=Fom-1 PE=4 SV=1)

HSP 1 Score: 488 bits (1257), Expect = 8.36e-163
Identity = 273/517 (52.80%), Postives = 346/517 (66.92%), Query Frame = 0

Query: 5   KLVAPGKYKTLVEMSRRLDKMNKLLDFDLELNDVRFVGIVGMGGIGKTTIAEVVYDDILL 64
           KLV   +   LV++ R+L KM KL D  LE N VRF+GI+GMGGIGKTTIAEV YD +  
Sbjct: 178 KLVPWDELTKLVDIERQLFKMEKLND--LEPNVVRFIGIIGMGGIGKTTIAEVFYDRVAR 237

Query: 65  GFWKNSCFLRITPERNLVTLQHQLLSQLLLEKDIEIRDEDQGAQLIKNRL---------- 124
            F KN CFLRI     L++LQ QLLSQLL  KD+ I +E++GA++I +RL          
Sbjct: 238 TFGKNRCFLRIYEHTTLLSLQQQLLSQLLQTKDLIINNENEGARMIGSRLKDKRVLIVLD 297

Query: 125 -------------------SGIKVLI----------------------------GAFSLF 184
                              SG K++I                             A +LF
Sbjct: 298 GVKEKTQLEQLVGNPNWFGSGSKIIITTRNRDVLRQPNYKDKMVEYSVEFLDTKSAMTLF 357

Query: 185 CKHAFGDGRPLDDNLKDLCNGIVRQTRRLPLALIKIGSSLYGQSIDIWREELESLTRAHN 244
           CKHAFG G P   N KD    IV + +  P ALI+IGSSLY + I+IW+EEL+SL   +N
Sbjct: 358 CKHAFGCGFPRK-NFKDFSKEIVERVKGHPQALIQIGSSLYDKGIEIWKEELKSLEEDYN 417

Query: 245 KRLFDTLKISFDDIGKTSQQVFLDFACFFNGKAIDRVIEILRSLGYMPHSEMQLLCDRCL 304
            R+F TLKISFDD+GKTSQ+VFLD ACFFN K  ++VIEIL+S  Y PHSE+QLL DRCL
Sbjct: 418 NRIFKTLKISFDDLGKTSQEVFLDLACFFNEKTKEKVIEILKSFDYRPHSEIQLLQDRCL 477

Query: 305 IEVGPQKTIIMPKFIQAMGQQIERDADRERSRIWLQEDIHTLFNE-RRGLKHIKGIILEL 364
           IEV    TI+MPK IQ MGQQIER+AD+ RSRIWL +D   +F+E  + +K IKG++L+L
Sbjct: 478 IEVRSDNTILMPKCIQTMGQQIEREADK-RSRIWLPKDAQDVFDEPHQRVKDIKGVVLKL 537

Query: 365 EENQDELELEGRLFTNMTCLRMLKIGNVQLRRDITNLSRELRLLHWHGYPLECLPSDFES 424
           EE QDE+ELEG++F +M  L++L+IGNV++  D T+LS++LRLL+WH YP +CLP  FES
Sbjct: 538 EEKQDEIELEGKVFEDMRSLKILEIGNVEVSGDFTHLSKQLRLLNWHSYPSQCLPLSFES 597

Query: 425 KYLFELLLPVGQTIQLWQGRKR-FQKLKVIDASDSKNLCETPDFSEVSNLERLILRNCTR 462
           +YLF+LLLP+ QT QLW G+K  F+KLKVI+ S SKNL ETP+F++V NLE L L NCTR
Sbjct: 598 RYLFQLLLPLSQTRQLWNGQKEGFEKLKVINVSGSKNLRETPNFTKVPNLESLDLSNCTR 657

BLAST of MC11g_new0432 vs. TAIR 10
Match: AT4G12010.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 209.5 bits (532), Expect = 6.4e-54
Identity = 156/532 (29.32%), Postives = 246/532 (46.24%), Query Frame = 0

Query: 7   VAPGKYKTLVEMSRRLDKMNKLLDFDLELNDVRFVGIVGMGGIGKTTIAEVVYDDILLGF 66
           +AP   + LV +  RL  + KLL ++ +L+ V  +GIVGM GIGKTT+A+ +Y   + G 
Sbjct: 179 LAPSGNEGLVGIESRLKNLEKLLSWE-DLDTVHIIGIVGMVGIGKTTLADCLYGR-MRGQ 238

Query: 67  WKNSCFLRITPERN----LVTLQHQLLSQLLLEKDIEIRDEDQGAQLIKNRLSGIKVLI- 126
           +  SCFL    E +    L +L  +L S +L ++D+EI       +  + RL   ++LI 
Sbjct: 239 FDGSCFLTNIRENSGRSGLESLLQKLFSTVLNDRDLEIGAPGNAHERFERRLKSKRLLIV 298

Query: 127 ----------------------------------------------------GAFSLFCK 186
                                                                A  LF  
Sbjct: 299 LDDVNDEKQIRYLMGHCKWYQGGSRIIITTRDSKLIETIKGRKYVLPKLNDREALKLFSL 358

Query: 187 HAFGDGRPLDDNLKDLCNGIVRQTRRLPLALIKIGSSLYGQSIDIWREELESLTRAHNKR 246
           +AF +  PL +  + L N ++   +  PLAL  +GS L  +    W  +L+ L    +  
Sbjct: 359 NAFSNSFPLKE-FEGLTNMVLDYAKGHPLALKVLGSDLCERDDLYWEAKLDRLKSRSHGD 418

Query: 247 LFDTLKISFDDIGKTSQQVFLDFACFFNGKAIDRVIEILRSLGYMPHSEMQLLCDRCLIE 306
           +++ L+ S++++    + VFLD ACFF  + +D V  +L S G      ++ L D+CLI 
Sbjct: 419 IYEVLETSYEELTTEQKNVFLDIACFFRSENVDYVTSLLNSHGVDVSGVVKDLVDKCLIT 478

Query: 307 VGPQKTIIMPKFIQAMGQQIERDAD-----------------RERSRIWLQEDIHTLFNE 366
           +   + I M   +Q M ++I    +                 +   R+W  EDI  L  E
Sbjct: 479 LSDNR-IEMHDMLQTMAKEISLKVETIGIRDCRWLSRHGNQCQWHIRLWDSEDICDLLTE 538

Query: 367 RRGLKHIKGIILELEENQDELELEGRLFTNMTCLRMLKIGN------------VQLRRDI 426
             G   I+GI L+  + +  + L  + F  M  L+ LKI +            + LRR +
Sbjct: 539 GLGTDKIRGIFLDTSKLR-AMRLSAKAFQGMYNLKYLKIYDSHCSRGCEAEFKLHLRRGL 598

Query: 427 TNLSRELRLLHWHGYPLECLPSDFESKYLFELLLPVGQTIQLWQGRKRFQKLKVIDASDS 453
           + L  EL  LHWHGYPL+ +P DF+ K L +L LP  Q  ++W   K    LK +D S S
Sbjct: 599 SFLPNELTYLHWHGYPLQSIPLDFDPKNLVDLKLPHSQLEEIWDDEKDVGMLKWVDLSHS 658

BLAST of MC11g_new0432 vs. TAIR 10
Match: AT1G72840.1 (Disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 200.7 bits (509), Expect = 3.0e-51
Identity = 161/535 (30.09%), Postives = 239/535 (44.67%), Query Frame = 0

Query: 15  LVEMSRRLDKMNKLLDFDLELNDVRFVGIVGMGGIGKTTIAEVVYDDILLGFWKNSCFL- 74
           LV M   + KM  LL+   E ++V  +GI GMGGIGK+TIA+ +YD     F    CFL 
Sbjct: 190 LVGMEAHMMKMTLLLNIGCE-DEVHMIGIWGMGGIGKSTIAKCLYDRFSRQF-PAHCFLE 249

Query: 75  RITPERNLVTLQHQLLSQLLLEKDIEIRDEDQGAQLIKNRLSGIKVLI------------ 134
            ++   ++  LQ +LLS +L ++D+E+   + G+Q IK RL   KV +            
Sbjct: 250 NVSKGYDIKHLQKELLSHILYDEDVELWSMEAGSQEIKERLGHQKVFVVLDNVDKVEQLH 309

Query: 135 ------------------------------------------GAFSLFCKHAFGDGRPLD 194
                                                      A  +F K AFG GRP  
Sbjct: 310 GLAKDPSWFGPGSRIIITTRDKGLLNSCGVNNIYEVKCLDDKDALQVFKKLAFG-GRPPS 369

Query: 195 DNLKDLCNGIVRQTRRLPLALIKIGSSLYG-QSIDIWREELESLTRAHNKRLFDTLKISF 254
           D  + L     R    LP AL+   S L    +ID W +EL  L     K + + L+ S+
Sbjct: 370 DGFEQLFIRASRLAHGLPSALVAFASHLSAIVAIDEWEDELALLETFPQKNVQEILRASY 429

Query: 255 DDIGKTSQQVFLDFACFFNGKAIDRVIEILRSLGYMPHSEMQLLCDRCLIEVGPQKTIIM 314
           D + +  + VFL  ACFFNG  +  +   L++      + +  L  +CL+ +     I M
Sbjct: 430 DGLDQYDKTVFLHVACFFNGGHLRYIRAFLKNC----DARINHLAAKCLVNISIDGCISM 489

Query: 315 PKFIQAMGQQIER-DADRERSR---IWLQEDIHTLFNERRGLKHIKGIILELEENQDELE 374
              +   G++I R ++D   S+   +W   +IH + +   G + ++G+ L L E  D L 
Sbjct: 490 HILLVQTGREIVRQESDWRPSKQRFLWDPTEIHYVLDSNTGTRRVEGLSLHLCEMADTLL 549

Query: 375 LEGRLFTNMTCLRMLK--------IGNVQLRRDITNLSRELRLLHWHGYPLECLPSDFES 434
           L   +F  M  L  LK        + N+QL  D   LSR L+LLHW  YPL  LP  F  
Sbjct: 550 LRNSVFGPMHNLTFLKFFQHLGGNVSNLQLISDDYVLSRNLKLLHWDAYPLTILPPIFRP 609

Query: 435 KYLFELLLPVGQTIQLWQGRKRFQKLKVIDASDSKNLCETPDFSEVSNLERLILRNCTRL 469
             + EL L   +   LW G K    L+++D + S+NL E P+ S   NLE LIL +CT L
Sbjct: 610 HTIIELSLRYSKLNSLWDGTKLLPNLRILDVTGSRNLRELPELSTAVNLEELILESCTSL 669

BLAST of MC11g_new0432 vs. TAIR 10
Match: AT1G72840.2 (Disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 200.7 bits (509), Expect = 3.0e-51
Identity = 161/535 (30.09%), Postives = 239/535 (44.67%), Query Frame = 0

Query: 15  LVEMSRRLDKMNKLLDFDLELNDVRFVGIVGMGGIGKTTIAEVVYDDILLGFWKNSCFL- 74
           LV M   + KM  LL+   E ++V  +GI GMGGIGK+TIA+ +YD     F    CFL 
Sbjct: 190 LVGMEAHMMKMTLLLNIGCE-DEVHMIGIWGMGGIGKSTIAKCLYDRFSRQF-PAHCFLE 249

Query: 75  RITPERNLVTLQHQLLSQLLLEKDIEIRDEDQGAQLIKNRLSGIKVLI------------ 134
            ++   ++  LQ +LLS +L ++D+E+   + G+Q IK RL   KV +            
Sbjct: 250 NVSKGYDIKHLQKELLSHILYDEDVELWSMEAGSQEIKERLGHQKVFVVLDNVDKVEQLH 309

Query: 135 ------------------------------------------GAFSLFCKHAFGDGRPLD 194
                                                      A  +F K AFG GRP  
Sbjct: 310 GLAKDPSWFGPGSRIIITTRDKGLLNSCGVNNIYEVKCLDDKDALQVFKKLAFG-GRPPS 369

Query: 195 DNLKDLCNGIVRQTRRLPLALIKIGSSLYG-QSIDIWREELESLTRAHNKRLFDTLKISF 254
           D  + L     R    LP AL+   S L    +ID W +EL  L     K + + L+ S+
Sbjct: 370 DGFEQLFIRASRLAHGLPSALVAFASHLSAIVAIDEWEDELALLETFPQKNVQEILRASY 429

Query: 255 DDIGKTSQQVFLDFACFFNGKAIDRVIEILRSLGYMPHSEMQLLCDRCLIEVGPQKTIIM 314
           D + +  + VFL  ACFFNG  +  +   L++      + +  L  +CL+ +     I M
Sbjct: 430 DGLDQYDKTVFLHVACFFNGGHLRYIRAFLKNC----DARINHLAAKCLVNISIDGCISM 489

Query: 315 PKFIQAMGQQIER-DADRERSR---IWLQEDIHTLFNERRGLKHIKGIILELEENQDELE 374
              +   G++I R ++D   S+   +W   +IH + +   G + ++G+ L L E  D L 
Sbjct: 490 HILLVQTGREIVRQESDWRPSKQRFLWDPTEIHYVLDSNTGTRRVEGLSLHLCEMADTLL 549

Query: 375 LEGRLFTNMTCLRMLK--------IGNVQLRRDITNLSRELRLLHWHGYPLECLPSDFES 434
           L   +F  M  L  LK        + N+QL  D   LSR L+LLHW  YPL  LP  F  
Sbjct: 550 LRNSVFGPMHNLTFLKFFQHLGGNVSNLQLISDDYVLSRNLKLLHWDAYPLTILPPIFRP 609

Query: 435 KYLFELLLPVGQTIQLWQGRKRFQKLKVIDASDSKNLCETPDFSEVSNLERLILRNCTRL 469
             + EL L   +   LW G K    L+++D + S+NL E P+ S   NLE LIL +CT L
Sbjct: 610 HTIIELSLRYSKLNSLWDGTKLLPNLRILDVTGSRNLRELPELSTAVNLEELILESCTSL 669

BLAST of MC11g_new0432 vs. TAIR 10
Match: AT4G19510.2 (Disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 198.0 bits (502), Expect = 1.9e-50
Identity = 151/503 (30.02%), Postives = 232/503 (46.12%), Query Frame = 0

Query: 21  RLDKMNKLLDFDLELND--VRFVGIVGMGGIGKTTIAEVVYDDILLGFWKNSCFLRITPE 80
           R  ++ KLL FD   ND  VR VG++GM GIGKTT+A++VY      F     FL    +
Sbjct: 198 RSKELEKLLMFD---NDECVRVVGVLGMTGIGKTTVADIVYKQNFQRF-DGYEFLEDIED 257

Query: 81  RN----LVTLQHQLLSQLLLEKDIEIRDEDQGAQLIKNRL-----------SGIKVLIG- 140
            +    L  L  +LL +LL  +++++R + +    ++N+              I+ LIG 
Sbjct: 258 NSKRYGLPYLYQKLLHKLLDGENVDVRAQGRPENFLRNKKLFIVLDNVTEEKQIEYLIGK 317

Query: 141 -------------------------------------AFSLFCKHAFGDGRPLDDNLKDL 200
                                                A  LFC   FG+  P ++   DL
Sbjct: 318 KNVYRQGSRIVIITRDKKLLQKNADATYVVPRLNDREAMELFCLQVFGNHYPTEE-FVDL 377

Query: 201 CNGIVRQTRRLPLALIKIGSSLYGQSIDIWREELESLTRAHNKRLFDTLKISFDDIGKTS 260
            N  V   + LPLAL  +G  L    I+ W+++LE L    +K L   LK S+  +    
Sbjct: 378 SNDFVCYAKGLPLALKLLGKGLLTHDINYWKKKLEFLQVNPDKELQKELKSSYKALDDDQ 437

Query: 261 QQVFLDFACFFNGKAIDRVIEILRSLGYMPHSEMQLLCDRCLIEVGPQKTIIMPKFIQAM 320
           + VFLD ACFF  +  D V  IL+S        M+ L ++CL+ +   + I M   + AM
Sbjct: 438 KSVFLDIACFFRSEKADFVSSILKSDDIDAKDVMRELEEKCLVTISYDR-IEMHDLLHAM 497

Query: 321 GQQIERDAD----RERSRIWLQEDIHTLFNERRGLKHIKGIILELEENQDELELEGRLFT 380
           G++I ++       ER R+W  +DI  +     G + ++GI L + E +  ++L    FT
Sbjct: 498 GKEIGKEKSIRKAGERRRLWNHKDIRDILEHNTGTECVRGIFLNMSEVR-RIKLFPAAFT 557

Query: 381 NMTCLRMLKIGN------------VQLRRDITNLSRELRLLHWHGYPLECLPSDFESKYL 440
            ++ L+ LK  +             Q  +   +   EL  LHW GYP +CLPSDF+ K L
Sbjct: 558 MLSKLKFLKFHSSHCSQWCDNDHIFQCSKVPDHFPDELVYLHWQGYPYDCLPSDFDPKEL 617

Query: 441 FELLLPVGQTIQLWQGRKRFQKLKVIDASDSKNLCETPDFSEVSNLERLILRNCTRLQKI 453
            +L L      QLW+  K  + L+ +D   SK+L      S   NLERL L  CT L  +
Sbjct: 618 VDLSLRYSHIKQLWEDEKNTESLRWVDLGQSKDLLNLSGLSRAKNLERLDLEGCTSLDLL 677

BLAST of MC11g_new0432 vs. TAIR 10
Match: AT5G51630.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 193.7 bits (491), Expect = 3.6e-49
Identity = 144/501 (28.74%), Postives = 237/501 (47.31%), Query Frame = 0

Query: 33  LELNDVRFVGIVGMGGIGKTTIAEVVYDDILLGFWKN--SCFLRITPERNLVTL--QHQL 92
           LE  + R VGI+G  GIGKTTIA ++Y  +   F  +    F R   +   + L  + Q 
Sbjct: 201 LESEEARMVGILGPSGIGKTTIARILYSKLSSQFDYHVFGSFKRTNQDNYGMKLSWEEQF 260

Query: 93  LSQLLLEKDIEIRDEDQGAQLIKNR-----------LSGIKVLIG--------------- 152
           LS++L +KD++I       Q +K++           L  +K L+G               
Sbjct: 261 LSEILDQKDLKISQLGVVKQRLKHKKVLIVLDDVDNLELLKTLVGQTGWFGPGSRIIVTT 320

Query: 153 ------------------------AFSLFCKHAFGDGRPLDDNLKDLCNGIVRQTRRLPL 212
                                   A  + C+ AF    P  D    L N +      LPL
Sbjct: 321 QDRILLKSHKIDHIYEVGYPSRKLALRILCRSAFDRNSP-PDGFMQLANEVTELVGNLPL 380

Query: 213 ALIKIGSSLYGQSIDIWREELESLTRA-HNKRLFDTLKISFDDIGKTSQQVFLDFACFFN 272
           AL  +GSSL G+  + W E + SL  +  +  +  TL++S+D +    Q++FL  AC  N
Sbjct: 381 ALNIMGSSLKGRDKEEWIEMMPSLRNSLVDGEILKTLRVSYDRLHGNYQEIFLYIACLLN 440

Query: 273 GKAIDRVIEILRSLGYMPHSEMQLLCDRCLIEVGP-QKTIIMPKFIQAMGQQIERDAD-- 332
              ++ +I +   LG      +++L ++ LI + P  KT+ M   +Q +G++I RD    
Sbjct: 441 CCGVEYIISM---LGDNAIIGLKILAEKSLIHISPLDKTVEMHSLLQKLGRKIVRDESFG 500

Query: 333 --RERSRIWLQEDIHTLFNERRGLKHIKGIILELEENQDELELEGRLFTNMTCLRMLKI- 392
              +R  +   EDI  +F +  G + + GI L   E    L ++ + F  M  L+ LK+ 
Sbjct: 501 NPGKRRFLLDAEDICDVFTDNTGTETVLGISLNTLEINGTLSVDDKSFQGMHNLQFLKVF 560

Query: 393 ---------GNVQLRRDITNLSRELRLLHWHGYPLECLPSDFESKYLFELLLPVGQTIQL 452
                    G + L + + +L R+LRLLHW+ +PL C+PS+F+++YL  L +   Q  +L
Sbjct: 561 ENWRRGSGEGILSLPQGLNSLPRKLRLLHWYKFPLRCMPSNFKAEYLVNLEMAYSQLERL 620

Query: 453 WQGRKRFQKLKVIDASDSKNLCETPDFSEVSNLERLILRNCTRLQKIDSSISTLSRLVLL 464
           W+G ++   LK +D S S+NL E PD S   NLE + L +C  L  + SS+  L +L +L
Sbjct: 621 WEGTQQLGSLKKMDLSKSENLKEIPDLSYAVNLEEMDLCSCKSLVTLPSSVRNLDKLRVL 680

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SZ669.0e-5329.32Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=... [more]
Q403923.2e-5029.37TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1[more]
V9M3981.2e-4931.17Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=... [more]
V9M2S51.6e-4931.12Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=... [more]
F4JT802.7e-4930.02Disease resistance protein RPP2B OS=Arabidopsis thaliana OX=3702 GN=RPP2B PE=1 S... [more]
Match NameE-valueIdentityDescription
XP_022141771.19.95e-17151.50TMV resistance protein N-like isoform X2 [Momordica charantia][more]
XP_022141770.15.16e-17054.42TMV resistance protein N-like isoform X1 [Momordica charantia][more]
XP_038891076.14.00e-16654.65disease resistance protein RPV1-like isoform X3 [Benincasa hispida] >XP_03889107... [more]
XP_038891073.14.25e-16654.65disease resistance protein RPV1-like isoform X2 [Benincasa hispida] >XP_03889107... [more]
XP_038891070.15.94e-16654.65disease resistance protein RPV1-like isoform X1 [Benincasa hispida] >XP_03889107... [more]
Match NameE-valueIdentityDescription
A0A6J1CK854.82e-17151.50TMV resistance protein N-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1CJ232.50e-17054.42TMV resistance protein N-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC11... [more]
Q5DMW25.55e-16452.62MRGH9 OS=Cucumis melo OX=3656 GN=MRGH9 PE=4 SV=1[more]
A0A1S3C9Z02.04e-16352.62TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103498649 PE=4 SV=1[more]
M4QSV98.36e-16352.80Fom-1 OS=Cucumis melo OX=3656 GN=Fom-1 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G12010.16.4e-5429.32Disease resistance protein (TIR-NBS-LRR class) family [more]
AT1G72840.13.0e-5130.09Disease resistance protein (TIR-NBS-LRR class) [more]
AT1G72840.23.0e-5130.09Disease resistance protein (TIR-NBS-LRR class) [more]
AT4G19510.21.9e-5030.02Disease resistance protein (TIR-NBS-LRR class) [more]
AT5G51630.13.6e-4928.74Disease resistance protein (TIR-NBS-LRR class) family [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 296..316
NoneNo IPR availablePRINTSPR00364DISEASERSISTcoord: 387..403
score: 27.06
coord: 40..55
score: 63.75
NoneNo IPR availablePANTHERPTHR11017:SF434REPEAT-CONTAINING PROTEIN, PUTATIVE-RELATEDcoord: 13..121
NoneNo IPR availablePANTHERPTHR11017:SF434REPEAT-CONTAINING PROTEIN, PUTATIVE-RELATEDcoord: 123..451
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 317..462
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 4..121
e-value: 3.5E-16
score: 61.2
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 12..211
IPR042197Apoptotic protease-activating factors, helical domainGENE3D1.10.8.430coord: 122..202
e-value: 2.1E-10
score: 42.1
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 284..471
e-value: 3.6E-13
score: 50.7
IPR002182NB-ARCPFAMPF00931NB-ARCcoord: 24..116
e-value: 4.9E-7
score: 29.2
IPR044974Disease resistance protein, plantsPANTHERPTHR11017LEUCINE-RICH REPEAT-CONTAINING PROTEINcoord: 13..121
IPR044974Disease resistance protein, plantsPANTHERPTHR11017LEUCINE-RICH REPEAT-CONTAINING PROTEINcoord: 123..451
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 190..289

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC11g_new0432.1MC11g_new0432.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
molecular_function GO:0043531 ADP binding