MC11g_new0199 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC11g_new0199
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	indole-3-acetic acid-amido synthetase GH3.6-like
Location	MC11: 7920345 .. 7923081 (+)
RNA-Seq Expression	MC11g_new0199
Synteny	MC11g_new0199

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAAAGAGAGTTTATATATAATATTTCTTAAATGGTTGGGGTACTGAATAAACTCATCATAAAAAAATGGGAAAAAGGAGAAATTTACCATTATACCCTTGTGGGTGTCTATATAAACCCACTTGCCTTTTTTTGTTTCTCCACACACCCAAAATCAAAACCCACCTTGTGGTCACCAAAAAAAACTCCCTCTTCCTATTCCTCTTCCTCTGTTTCTCTCACAAACGCTTTCCAAAAACCATCTCTTTCAAGCTCAAAGAGCCATGCCTGAAGAAGCTCCTCCAAACACCAACACCACCGACCACTACAAACTCCTCGAGAAGAACAAGAAGAGCCTTCAATTCATTGAAGATGTCACTGCCAACGCCGACCAAGTCCAGCGCCGCGTCCTCGCCGAAATCCTCTCCCGCAATGCTGGCGTCGAGTACCTCCGCCGCCACGGCCTCCCCGCCCACGCCGATCGCGACACCTTCAAAAACACCCTCCCGGTCATCTCCTACGAGGACATTCAGCCCGACATCAACCGCATCGCCAATGGCGACTCCTCTCCCATCCTCTGCTCTTCCCCCATTTCCGAGTTCCTCACCAGGTACTTCCTCCAATACCAATGCATAAGTTTTTGGATTTGAACGGTGGTTTATTCATAGATGCAATGTTATTCCATTCCATTTATTTGGTCTTGTAATAATTTTTCAGGCCCACAAAATTTAACCAACTCATCCGTTTAAACTTTTGGACCGAGTGATGTTTTATCCATCATCTACATCTTTTTCTCTCTTTTCCTTACATGGGTCTCTGCTTTTGCAGCTCGGGGACGTCGGGAGGGGAGCGAAAGCTGATGCCGACGATCGAGGAGGAGCTGGAGAGGAGATCATTGCTGTACAGCCTGCTGATGCCGGTGATGAGCCAATTCGTGGGAGGGCTAGAGAAGGGCAAAGGAATGTACTTCCTGTTCGTGAAATCAGAGGCCAAAACGCCGAGTGGGCTATTGGCGCGCCCTGTTCTCACAAGCTACTACAAAAGCCCCCATTTTAAGCACCGCCCATTGGACCCTTACACTAACTACACGAGCCCCAACGAGGCCATCCTCTGCCCCGACGCCTACCAGAGCATGTACGCCCAATTGCTCGCCGGCCTCCACCACCGCGCCGCCGTCCTCCGCGTCGGCGCCGTCTTCGCCTCCGGCTTCATCCGCGCCCTCCGCTTCCTCCAAAACCACTTCCCCCTCCTCTGCCACGACCTCCGCACCGGCACCCTCAACCCGCAGATCACCGATCACCCCCTCCGCGAATCCCTCCGCCGGATCCTCCTCAAACCCGACCCGAAACTGGCGGACCTCGTGGAGTCCGAGTGCAGCAAGAAGTCGTGGCAGGGGATTATTACCCGCCTCTGGCCCAACACCAAGTATGTGGATGTTATTGTGACGGGGACAATGTCGCAGTATATTCCGACGCTGGATTATTACAGCAACGGCCTGCCGCTGGTCTGCACCATGTATGCTTCCTCCGAGTGCTATTTTGGGGTTAATTTGAATCCTCTGTGTAAGCCCTCTGAGGTTTCTTATACGCTGATTCCCACAATGGCCTATTTCGAGTTCCTCCCTGTCGATAGAAACATGTCCTTGAATGACAACGACTTGCACCGCCAGCTCGTCGACCTCGCCGATGTTCGGCTTGGACACGACTACGAGCTGGTTGTTACAACCTATGCAGGTATGGAGTTTTCTTAAAGAAAAATTTAAGAATCTATTAGACACATAAATTGAAAATGATTATGAGCACTCTAATCCATGTACAGGATTGTACCGGTATAGAGTGGGGGACGTTCTACGAGTCGCGGGGTTCAAGAACAATGCGCCGCAGTTCAACTTCATATGCAGGAAAAACGTGGTGTTGAGCATAGATTCGGATAAGACAGACGAGGTGGAGCTTCAAAACGCTATGAAAAACGCAGTGAGTCAGCACCTAATGCCGTTCGATGCGAGAGTGAGCGAGTACACGAGCTGTGTGAGCACCTCAAAAATCCCTGGTCACTACGTCATGTATTGGGAGCTGACTGTGAACGGAGCCACACCGATCCCACCCTCCATCTTTGAGGACTGTTGCTTGACAATTGAGGAGTCTCTCAACAGCGTGTACCGTCAGGGTCGGGTCTCGGATAAGTCGATCGGGGCACTCGAGATCAAGGTGGTCGAGGCGGGGACGTTCGACAAGCTCATGGACTATGCCATCAGCCTAGGGGCATCAATAAACCAGTATAAGACACCAAGATGTGTGAAGTATGAACCCATCATTCAGCTATTGAACTCAAGGGTTGTGTCAAATTACTTCAGCCCCAAGTGCCCAAAATGGGTTCCAGGTCATAAGCAATGGAATTGAATTGGAATTTGGAAAGGACATTTTTAGGGTTACTTTTTTCATGGAGGTCCTCACAAAATAATTTAACTTGTCAATTTATCTAGGGAAAAATTTAATCACCCTAGATCTCTCTCTCTCTCTTTTTTTGTTTAGAACTCATGTTTCTTAATTAGCTTCTCTAATCTTGTTCTTCTGTATCCTTTCTGAAAATTTTAGCAAGCTGTTGGAAATGGGTAGACTTTGAACTAGACCAATGTACAAAAACAGTTCTTGTTCATGCAAGTAATCTTCTTCTTCTTTTTTTCTCTCCTAAATTTTCCTTAGATAACAAATTTGGTCTTTTTGATTTGGGTATGGTTTAAATTTTAGTCCAATTTAGC

mRNA sequence

ATGCCTGAAGAAGCTCCTCCAAACACCAACACCACCGACCACTACAAACTCCTCGAGAAGAACAAGAAGAGCCTTCAATTCATTGAAGATGTCACTGCCAACGCCGACCAAGTCCAGCGCCGCGTCCTCGCCGAAATCCTCTCCCGCAATGCTGGCGTCGAGTACCTCCGCCGCCACGGCCTCCCCGCCCACGCCGATCGCGACACCTTCAAAAACACCCTCCCGGTCATCTCCTACGAGGACATTCAGCCCGACATCAACCGCATCGCCAATGGCGACTCCTCTCCCATCCTCTGCTCTTCCCCCATTTCCGAGTTCCTCACCAGCTCGGGGACGTCGGGAGGGGAGCGAAAGCTGATGCCGACGATCGAGGAGGAGCTGGAGAGGAGATCATTGCTGTACAGCCTGCTGATGCCGGTGATGAGCCAATTCGTGGGAGGGCTAGAGAAGGGCAAAGGAATGTACTTCCTGTTCGTGAAATCAGAGGCCAAAACGCCGAGTGGGCTATTGGCGCGCCCTGTTCTCACAAGCTACTACAAAAGCCCCCATTTTAAGCACCGCCCATTGGACCCTTACACTAACTACACGAGCCCCAACGAGGCCATCCTCTGCCCCGACGCCTACCAGAGCATGTACGCCCAATTGCTCGCCGGCCTCCACCACCGCGCCGCCGTCCTCCGCGTCGGCGCCGTCTTCGCCTCCGGCTTCATCCGCGCCCTCCGCTTCCTCCAAAACCACTTCCCCCTCCTCTGCCACGACCTCCGCACCGGCACCCTCAACCCGCAGATCACCGATCACCCCCTCCGCGAATCCCTCCGCCGGATCCTCCTCAAACCCGACCCGAAACTGGCGGACCTCGTGGAGTCCGAGTGCAGCAAGAAGTCGTGGCAGGGGATTATTACCCGCCTCTGGCCCAACACCAAGTATGTGGATGTTATTGTGACGGGGACAATGTCGCAGTATATTCCGACGCTGGATTATTACAGCAACGGCCTGCCGCTGGTCTGCACCATGTATGCTTCCTCCGAGTGCTATTTTGGGGTTAATTTGAATCCTCTGTGTAAGCCCTCTGAGGTTTCTTATACGCTGATTCCCACAATGGCCTATTTCGAGTTCCTCCCTGTCGATAGAAACATGTCCTTGAATGACAACGACTTGCACCGCCAGCTCGTCGACCTCGCCGATGTTCGGCTTGGACACGACTACGAGCTGGTTGTTACAACCTATGCAGGATTGTACCGGTATAGAGTGGGGGACGTTCTACGAGTCGCGGGGTTCAAGAACAATGCGCCGCAGTTCAACTTCATATGCAGGAAAAACGTGGTGTTGAGCATAGATTCGGATAAGACAGACGAGGTGGAGCTTCAAAACGCTATGAAAAACGCAGTGAGTCAGCACCTAATGCCGTTCGATGCGAGAGTGAGCGAGTACACGAGCTGTGTGAGCACCTCAAAAATCCCTGGTCACTACGTCATGTATTGGGAGCTGACTGTGAACGGAGCCACACCGATCCCACCCTCCATCTTTGAGGACTGTTGCTTGACAATTGAGGAGTCTCTCAACAGCGTGTACCGTCAGGGTCGGGTCTCGGATAAGTCGATCGGGGCACTCGAGATCAAGGTGGTCGAGGCGGGGACGTTCGACAAGCTCATGGACTATGCCATCAGCCTAGGGGCATCAATAAACCAGTATAAGACACCAAGATGTGTGAAGTATGAACCCATCATTCAGCTATTGAACTCAAGGGTTGTGTCAAATTACTTCAGCCCCAAGTGCCCAAAATGGGTTCCAGGTCATAAGCAATGGAATTGA

Coding sequence (CDS)

ATGCCTGAAGAAGCTCCTCCAAACACCAACACCACCGACCACTACAAACTCCTCGAGAAGAACAAGAAGAGCCTTCAATTCATTGAAGATGTCACTGCCAACGCCGACCAAGTCCAGCGCCGCGTCCTCGCCGAAATCCTCTCCCGCAATGCTGGCGTCGAGTACCTCCGCCGCCACGGCCTCCCCGCCCACGCCGATCGCGACACCTTCAAAAACACCCTCCCGGTCATCTCCTACGAGGACATTCAGCCCGACATCAACCGCATCGCCAATGGCGACTCCTCTCCCATCCTCTGCTCTTCCCCCATTTCCGAGTTCCTCACCAGCTCGGGGACGTCGGGAGGGGAGCGAAAGCTGATGCCGACGATCGAGGAGGAGCTGGAGAGGAGATCATTGCTGTACAGCCTGCTGATGCCGGTGATGAGCCAATTCGTGGGAGGGCTAGAGAAGGGCAAAGGAATGTACTTCCTGTTCGTGAAATCAGAGGCCAAAACGCCGAGTGGGCTATTGGCGCGCCCTGTTCTCACAAGCTACTACAAAAGCCCCCATTTTAAGCACCGCCCATTGGACCCTTACACTAACTACACGAGCCCCAACGAGGCCATCCTCTGCCCCGACGCCTACCAGAGCATGTACGCCCAATTGCTCGCCGGCCTCCACCACCGCGCCGCCGTCCTCCGCGTCGGCGCCGTCTTCGCCTCCGGCTTCATCCGCGCCCTCCGCTTCCTCCAAAACCACTTCCCCCTCCTCTGCCACGACCTCCGCACCGGCACCCTCAACCCGCAGATCACCGATCACCCCCTCCGCGAATCCCTCCGCCGGATCCTCCTCAAACCCGACCCGAAACTGGCGGACCTCGTGGAGTCCGAGTGCAGCAAGAAGTCGTGGCAGGGGATTATTACCCGCCTCTGGCCCAACACCAAGTATGTGGATGTTATTGTGACGGGGACAATGTCGCAGTATATTCCGACGCTGGATTATTACAGCAACGGCCTGCCGCTGGTCTGCACCATGTATGCTTCCTCCGAGTGCTATTTTGGGGTTAATTTGAATCCTCTGTGTAAGCCCTCTGAGGTTTCTTATACGCTGATTCCCACAATGGCCTATTTCGAGTTCCTCCCTGTCGATAGAAACATGTCCTTGAATGACAACGACTTGCACCGCCAGCTCGTCGACCTCGCCGATGTTCGGCTTGGACACGACTACGAGCTGGTTGTTACAACCTATGCAGGATTGTACCGGTATAGAGTGGGGGACGTTCTACGAGTCGCGGGGTTCAAGAACAATGCGCCGCAGTTCAACTTCATATGCAGGAAAAACGTGGTGTTGAGCATAGATTCGGATAAGACAGACGAGGTGGAGCTTCAAAACGCTATGAAAAACGCAGTGAGTCAGCACCTAATGCCGTTCGATGCGAGAGTGAGCGAGTACACGAGCTGTGTGAGCACCTCAAAAATCCCTGGTCACTACGTCATGTATTGGGAGCTGACTGTGAACGGAGCCACACCGATCCCACCCTCCATCTTTGAGGACTGTTGCTTGACAATTGAGGAGTCTCTCAACAGCGTGTACCGTCAGGGTCGGGTCTCGGATAAGTCGATCGGGGCACTCGAGATCAAGGTGGTCGAGGCGGGGACGTTCGACAAGCTCATGGACTATGCCATCAGCCTAGGGGCATCAATAAACCAGTATAAGACACCAAGATGTGTGAAGTATGAACCCATCATTCAGCTATTGAACTCAAGGGTTGTGTCAAATTACTTCAGCCCCAAGTGCCCAAAATGGGTTCCAGGTCATAAGCAATGGAATTGA

Protein sequence

MPEEAPPNTNTTDHYKLLEKNKKSLQFIEDVTANADQVQRRVLAEILSRNAGVEYLRRHGLPAHADRDTFKNTLPVISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSEAKTPSGLLARPVLTSYYKSPHFKHRPLDPYTNYTSPNEAILCPDAYQSMYAQLLAGLHHRAAVLRVGAVFASGFIRALRFLQNHFPLLCHDLRTGTLNPQITDHPLRESLRRILLKPDPKLADLVESECSKKSWQGIITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVSYTLIPTMAYFEFLPVDRNMSLNDNDLHRQLVDLADVRLGHDYELVVTTYAGLYRYRVGDVLRVAGFKNNAPQFNFICRKNVVLSIDSDKTDEVELQNAMKNAVSQHLMPFDARVSEYTSCVSTSKIPGHYVMYWELTVNGATPIPPSIFEDCCLTIEESLNSVYRQGRVSDKSIGALEIKVVEAGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVPGHKQWN

Homology

[Close]

BLAST of MC11g_new0199 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 7.2e-280
Identity = 475/611 (77.74%), Postives = 523/611 (85.60%), Query Frame = 0


Query: 1   MPEEAPPNTNTTDHYKLLEKNKKSLQFIEDVTANADQVQRRVLAEILSRNAGVEYLRRHG 60
           MPE             L EKNK  LQFIEDVT NAD VQRRVL EILSRNA VEYL+RHG
Sbjct: 1   MPEAPKIAALEVSDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHG 60

Query: 61  LPAHADRDTFKNTLPVISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGERKLM 120
           L    DR+TFK+ +PV++YEDIQP+INRIANGD S +LCS+PISEFLTSSGTSGGERKLM
Sbjct: 61  LEGRTDRETFKHIMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLM 120

Query: 121 PTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSEAKTPSGLLARPVLTSYYK 180
           PTIEEEL+RRSLLYSLLMPVM QFV GL+KGKGMYFLF+KSE+KTP GL ARPVLTSYYK
Sbjct: 121 PTIEEELDRRSLLYSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYK 180

Query: 181 SPHFKHRPLDPYTNYTSPNEAILCPDAYQSMYAQLLAGLHHRAAVLRVGAVFASGFIRAL 240
           S HFK+RP DPYTNYTSPN+ ILC D+YQSMY+Q+L GL     VLRVGAVFASGFIRA+
Sbjct: 181 SSHFKNRPYDPYTNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAI 240

Query: 241 RFLQNHFPLLCHDLRTGTLNPQITDHPLRESLRRILLKPDPKLADLVESECSKKSWQGII 300
           +FL+ H+P L  D+RTGTL+ +ITD  +RE++  I LKPDPKLAD VESEC K SWQGII
Sbjct: 241 KFLEKHWPELARDIRTGTLSSEITDSSVREAVGEI-LKPDPKLADFVESECRKTSWQGII 300

Query: 301 TRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVS 360
           TRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNL PLCKPSEVS
Sbjct: 301 TRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVS 360

Query: 361 YTLIPTMAYFEFLPVDRNMSLNDN---------DLHRQLVDLADVRLGHDYELVVTTYAG 420
           YTLIP MAYFEFLPV RN  +  +            ++LVDL DV+LG +YELVVTTYAG
Sbjct: 361 YTLIPNMAYFEFLPVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAG 420

Query: 421 LYRYRVGDVLRVAGFKNNAPQFNFICRKNVVLSIDSDKTDEVELQNAMKNAVSQHLMPFD 480
           LYRYRVGDVL VAGFKNNAPQF+FICRKNVVLSIDSDKTDEVELQNA+KNAV+ HL+PFD
Sbjct: 421 LYRYRVGDVLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVT-HLVPFD 480

Query: 481 ARVSEYTSCVSTSKIPGHYVMYWELTVNGATPIPPSIFEDCCLTIEESLNSVYRQGRVSD 540
           A +SEYTS   TS IPGHYV++WEL +NG TPIPPS+FEDCCLTIEESLNSVYRQGRVSD
Sbjct: 481 ASLSEYTSYADTSSIPGHYVLFWELCLNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSD 540

Query: 541 KSIGALEIKVVEAGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPK 600
           KSIG LEIK+VE+GTFDKLMDYAISLGASINQYKTPRCVK+ PII+LLNSRVV +YFSPK
Sbjct: 541 KSIGPLEIKMVESGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPK 600

Query: 601 CPKWVPGHKQW 603
           CPKW PGHKQW
Sbjct: 601 CPKWSPGHKQW 609

[Close]

BLAST of MC11g_new0199 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 938.3 bits (2424), Expect = 4.2e-272
Identity = 464/611 (75.94%), Postives = 518/611 (84.78%), Query Frame = 0


Query: 1   MPEEAPPNTNTTDHYKLLEKNKKSLQFIEDVTANADQVQRRVLAEILSRNAGVEYLRRHG 60
           MPE     +       L +KNK+ LQ IE++T+NADQVQR+VL EIL+RNA VEYLRRH 
Sbjct: 1   MPEAPKKESLEVFDLTLDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHD 60

Query: 61  LPAHADRDTFKNTLPVISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGERKLM 120
           L    DR+TFKN +PVI+YEDI+P+INRIANGD SPIL S PISEFLTSSGTSGGERKLM
Sbjct: 61  LNGRTDRETFKNIMPVITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLM 120

Query: 121 PTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSEAKTPSGLLARPVLTSYYK 180
           PTIEEEL+RRSLLYSLLMPVMSQFV GLE GKGMYFLF+KSE+KTP GL ARPVLTSYYK
Sbjct: 121 PTIEEELDRRSLLYSLLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYK 180

Query: 181 SPHFKHRPLDPYTNYTSPNEAILCPDAYQSMYAQLLAGLHHRAAVLRVGAVFASGFIRAL 240
           S HFK RP DPYTNYTSPNE ILC D+YQSMY+Q+L GL     VLRVGAVFASGFIRA+
Sbjct: 181 SSHFKERPYDPYTNYTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAI 240

Query: 241 RFLQNHFPLLCHDLRTGTLNPQITDHPLRESLRRILLKPDPKLADLVESECSKKSWQGII 300
           +FL+ H+  L  D+RTGTL+  ITD  +RE++ +I LKP PKLAD VE EC K SWQGII
Sbjct: 241 KFLEKHWIELVRDIRTGTLSSLITDPSVREAVAKI-LKPSPKLADFVEFECKKSSWQGII 300

Query: 301 TRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVS 360
           TRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNL PLCKPSEVS
Sbjct: 301 TRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVS 360

Query: 361 YTLIPTMAYFEFLPVDRNMSLNDN---------DLHRQLVDLADVRLGHDYELVVTTYAG 420
           YTLIP+MAYFEFLPV RN  + ++            ++LVDL DV+LG +YELVVTTYAG
Sbjct: 361 YTLIPSMAYFEFLPVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAG 420

Query: 421 LYRYRVGDVLRVAGFKNNAPQFNFICRKNVVLSIDSDKTDEVELQNAMKNAVSQHLMPFD 480
           L RYRVGD+LRV GFKN APQF+FICRKNVVLSIDSDKTDEVELQNA+KNAV+ HL+PFD
Sbjct: 421 LCRYRVGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVT-HLVPFD 480

Query: 481 ARVSEYTSCVSTSKIPGHYVMYWELTVNGATPIPPSIFEDCCLTIEESLNSVYRQGRVSD 540
           A +SEYTS   TS IPGHYV++WEL ++G TPIPPS+FEDCCL +EES N+VYRQGRVSD
Sbjct: 481 ASLSEYTSYADTSSIPGHYVLFWELCLDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSD 540

Query: 541 KSIGALEIKVVEAGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPK 600
           KSIG LEIK+VE GTFDKLMDYAISLGASINQYKTPRCVK+ PII+LLNSRVV +YFSPK
Sbjct: 541 KSIGPLEIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPK 600

Query: 601 CPKWVPGHKQW 603
           CPKWVPGHKQW
Sbjct: 601 CPKWVPGHKQW 609

[Close]

BLAST of MC11g_new0199 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 829.3 bits (2141), Expect = 2.7e-239
Identity = 406/605 (67.11%), Postives = 487/605 (80.50%), Query Frame = 0


Query: 1   MPEEAPPNTNTTDHYKLLE-KNKKSLQFIEDVTANADQVQRRVLAEILSRNAGVEYLRRH 60
           MPE   P   T   Y      + ++L+FIE VTANA QVQRRVL EIL++NA  EYLRR+
Sbjct: 1   MPE--APTAKTAPAYGYAPGAHAEALEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRY 60

Query: 61  GLPAHAD-RDTFKNTLPVISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGERK 120
           G+P   D  D F+  +P+++YE +QPDI RIANGD+SPI    PISEFLTSSGTSGGERK
Sbjct: 61  GIPGSPDVVDAFRRLVPLVTYEGLQPDILRIANGDTSPIFSGKPISEFLTSSGTSGGERK 120

Query: 121 LMPTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSEAKTPSGLLARPVLTSY 180
           LMPTI +E+ RRSLLYSLLMPVMSQ V GL+KGK MY LFVK+E++TP GL ARPVLTSY
Sbjct: 121 LMPTIADEMNRRSLLYSLLMPVMSQSVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSY 180

Query: 181 YKSPHFKHRPLDPYTNYTSPNEAILCPDAYQSMYAQLLAGLHHRAAVLRVGAVFASGFIR 240
           Y+S  F  RP DPYT+YTSP+EAILC D+YQSMYAQLL GL HRA VLRVGAVFASGF+R
Sbjct: 181 YRSRQFLDRPRDPYTSYTSPDEAILCVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLR 240

Query: 241 ALRFLQNHFPLLCHDLRTGTLNPQITDHPLRESLRRILLKPDPKLADLVESECSKKSWQG 300
           A+ FL+ H+  LCHD+RTG L+P+ITD  +R+++ R+ L+ DP LAD +E EC++ SW+G
Sbjct: 241 AIHFLEKHWARLCHDIRTGELDPEITDRVVRDAVGRV-LRADPALADAIEDECARASWEG 300

Query: 301 IITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSE 360
           II RLWP TKY+DVIVTGTMSQYIPTL++Y  GLPL CTMYASSECYFG+NLNP+CKPS+
Sbjct: 301 IIRRLWPRTKYIDVIVTGTMSQYIPTLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSD 360

Query: 361 VSYTLIPTMAYFEFLPVDRNMSLNDNDLHRQLVDLADVRLGHDYELVVTTYAGLYRYRVG 420
           V+YTLIPTM Y+EFLPV+ N +  +   HR LVDL DV+LGH+YELVVTTY+GLYRYRVG
Sbjct: 361 VAYTLIPTMCYYEFLPVNCNNATAEAS-HRDLVDLVDVKLGHEYELVVTTYSGLYRYRVG 420

Query: 421 DVLRVAGFKNNAPQFNFICRKNVVLSIDSDKTDEVELQNAMKNAVSQHLMPFDARVSEYT 480
           DVLRVAGFKN AP F+F+ R+NV LS+DSDKTDE EL  A+  AV QHL PF A + EYT
Sbjct: 421 DVLRVAGFKNKAPMFSFVRRQNVALSVDSDKTDETELHAAVSGAV-QHLAPFGASLVEYT 480

Query: 481 SCVSTSKIPGHYVMYWELTVNGATPIPPSIFEDCCLTIEESLNSVYRQGRVSDKSIGALE 540
           S    + IPGHYV++WEL   G+T +P S+FE+CCL++EE+LNSVYRQGR  D+SIG LE
Sbjct: 481 SYADAATIPGHYVLFWELRA-GSTAVPASVFEECCLSVEEALNSVYRQGRACDRSIGPLE 540

Query: 541 IKVVEAGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVPG 600
           I+VV  GTFDKLMDYAIS GASINQYK PRCV+  P+++LL++RV   YFSPKCPKW PG
Sbjct: 541 IRVVAEGTFDKLMDYAISRGASINQYKAPRCVRPGPVVELLDARVQGKYFSPKCPKWSPG 599

Query: 601 HKQWN 604
           +KQWN
Sbjct: 601 NKQWN 599

[Close]

BLAST of MC11g_new0199 vs. ExPASy Swiss-Prot
Match: Q60EJ6 (Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.4 PE=2 SV=1)

HSP 1 Score: 792.7 bits (2046), Expect = 2.9e-228
Identity = 396/592 (66.89%), Postives = 470/592 (79.39%), Query Frame = 0


Query: 20  KNKKSLQFIEDVTANADQVQRRVLAEILSRNAGVEYLRRHGLPAHAD--RDTFKNTLPVI 79
           ++++ L+ IE VT NA Q QRRVL EIL++NA  EYLRR G+P  A    + F+   P++
Sbjct: 22  EHREKLEHIERVTRNAGQEQRRVLEEILAQNAQAEYLRRLGVPGDAPGADEAFRRLAPLV 81

Query: 80  SYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSLL 139
           +YEDI PD+ RIANGD+SPIL   P+SEFLTSSGTSGGERKLMPTIEEE+ERRS LYSLL
Sbjct: 82  TYEDILPDVLRIANGDTSPILSGKPVSEFLTSSGTSGGERKLMPTIEEEMERRSGLYSLL 141

Query: 140 MPVMSQFVGGLEKGKGMYFLFVKSEAKTPSGLLARPVLTSYYKSPHFKHRPLDPYTNYTS 199
           MPVMS+ V GL+KGK MY  FVKSE +TP GL ARPVLTS+Y+S +F  RP DPYT YTS
Sbjct: 142 MPVMSRQVPGLDKGKAMYLYFVKSEWRTPGGLPARPVLTSFYRSRYFLERPHDPYTVYTS 201

Query: 200 PNEAILCPDAYQSMYAQLLAGLHHRAAVLRVGAVFASGFIRALRFLQNHFPLLCHDLRTG 259
           P+EA+LC DAYQSMYAQL+ GL HRA VLRVGAVFASGF+RA+RFL+ H+P LC D+R G
Sbjct: 202 PDEAVLCEDAYQSMYAQLICGLVHRADVLRVGAVFASGFLRAIRFLEKHWPSLCRDIRAG 261

Query: 260 TLNPQITDHPLRESLRRILLKPDPKLADLVESECSKKSWQGIITRLWPNTKYVDVIVTGT 319
            L+  +TD  +R ++ R+L   DP LAD +E+EC++ SWQGII R+WP+TKY+DVIVTG 
Sbjct: 262 ELDGGVTDPAVRGAVGRVLRGADPALADAIEAECARPSWQGIIRRVWPSTKYIDVIVTGA 321

Query: 320 MSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVSYTLIPTMAYFEFLPVDR 379
           M+QYIPTL++Y  GLPL CTMYASSECYFG+NLNP+CKPSEV+YTLIPTM YFEFLPV  
Sbjct: 322 MAQYIPTLEFYGGGLPLACTMYASSECYFGLNLNPMCKPSEVAYTLIPTMCYFEFLPV-- 381

Query: 380 NMSLND----NDLHRQLVDLADVRLGHDYELVVTTYAGLYRYRVGDVLRVAGFKNNAPQF 439
           N   ND       HR LVDL DV+LGH+YELVVTTY+GLYRYRVGDVLRVAGFKN AP F
Sbjct: 382 NSGANDVAAPEPDHRGLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNAAPMF 441

Query: 440 NFICRKNVVLSIDSDKTDEVELQNAMKNAVSQHLMPFDARVSEYTSCVST-SKIPGHYVM 499
            F+ RKNV LSIDSDKTDE EL  A+  AV QHL PF A + EYTS   T + IPGHYV+
Sbjct: 442 AFVRRKNVALSIDSDKTDEAELHAAVTEAV-QHLAPFGASLVEYTSYADTATTIPGHYVL 501

Query: 500 YWEL-TVNGATPIPPSIFEDCCLTIEESLNSVYRQGRVSDKSIGALEIKVVEAGTFDKLM 559
           +WEL +  G TP+P S+FEDCCL +EE LNSVYRQ R +D+SIG LEI+VV  GTFDKLM
Sbjct: 502 FWELRSPAGGTPVPASVFEDCCLAVEEGLNSVYRQCRAADRSIGPLEIRVVADGTFDKLM 561

Query: 560 DYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKW-VPGHKQW 603
           DYA+S GASINQYK PRCV+  P+++LL+ RV + YFSPKCPKW   G+KQW
Sbjct: 562 DYALSRGASINQYKAPRCVRPGPVVELLDGRVQATYFSPKCPKWCAGGNKQW 610

[Close]

BLAST of MC11g_new0199 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 4.9e-220
Identity = 380/584 (65.07%), Postives = 455/584 (77.91%), Query Frame = 0


Query: 19  EKNKKSLQFIEDVTANADQVQRRVLAEILSRNAGVEYLRRHGLPAHADRDTFKNTLPVIS 78
           EK+ K+L+FIE++T NAD VQ  +LAEIL+RNA  EYLRR  L    DRDTFK  +PVI+
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76

Query: 79  YEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSLLM 138
           YED+QP+I RIA+GD SPIL + PISEFLTSSGTS GERKLMPTI+EEL+RR LLYSLLM
Sbjct: 77  YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136

Query: 139 PVMSQFVGGLEKGKGMYFLFVKSEAKTPSGLLARPVLTSYYKSPHFKHRPLDPYTNYTSP 198
           PVM+ +V GL+KGKGMYFLFVKSE KTP GL ARPVLTSYYKS HF+ RP DPY  YTSP
Sbjct: 137 PVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTSP 196

Query: 199 NEAILCPDAYQSMYAQLLAGLHHRAAVLRVGAVFASGFIRALRFLQNHFPLLCHDLRTGT 258
           NEAILCPD++QSMY Q+L GL  R +VLRVGAVFASG +RA+RFLQ H+    HD+  G 
Sbjct: 197 NEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGC 256

Query: 259 LNPQITDHPLRESLRRILLKPDPKLADLVESECSKKSWQGIITRLWPNTKYVDVIVTGTM 318
           L+ +ITD  +R+ +  I LKPDP LA+ +  EC   +W+ IITR+WPNTKY+DVIVTG M
Sbjct: 257 LDSEITDPSIRQCMSGI-LKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAM 316

Query: 319 SQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVSYTLIPTMAYFEFLPVDRN 378
           +QYIPTL+YYS GLP+ CTMYASSECYFG+NLNP+ KPSEVSYT++P MAYFEF+P+   
Sbjct: 317 AQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLGGT 376

Query: 379 MSLNDNDLHRQLVDLADVRLGHDYELVVTTYAGLYRYRVGDVLRVAGFKNNAPQFNFICR 438
                     + V+L DV +G +YELVVTTYAGL RYRVGD+LRV GF N+APQF+F+ R
Sbjct: 377 ----------KAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRR 436

Query: 439 KNVVLSIDSDKTDEVELQNAMKNAVSQHLMPFDARVSEYTSCVSTSKIPGHYVMYWELTV 498
           KNV+LSIDSDKTDE ELQ A++NA S       +RV+EYTS   TS IPGHYV+YWEL V
Sbjct: 437 KNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELLV 496

Query: 499 -NGATPIPPSIFEDCCLTIEESLNSVYRQGRVSDKSIGALEIKVVEAGTFDKLMDYAISL 558
            +GA          CCL +EESLNSVYRQ RV+D S+G LEI+VV  GTF++LMDYAIS 
Sbjct: 497 RDGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAISR 556

Query: 559 GASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVPGHKQ 602
           GASINQYK PRCV + PI++LL+SRVVS +FSP  P W P  ++
Sbjct: 557 GASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERRR 589

[Close]

BLAST of MC11g_new0199 vs. NCBI nr
Match: XP_022139392.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia])

HSP 1 Score: 1210 bits (3131), Expect = 0.0
Identity = 593/603 (98.34%), Postives = 597/603 (99.00%), Query Frame = 0


Query: 1   MPEEAPPNTNTTDHYKLLEKNKKSLQFIEDVTANADQVQRRVLAEILSRNAGVEYLRRHG 60
           MPEEAPPNTNTTDHYKLLEKNKKSLQFIEDVTANADQVQRRVLAEILSRNAGVEYLRRHG
Sbjct: 1   MPEEAPPNTNTTDHYKLLEKNKKSLQFIEDVTANADQVQRRVLAEILSRNAGVEYLRRHG 60

Query: 61  LPAHADRDTFKNTLPVISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGERKLM 120
           LPAHADRDTFKNTLPVISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGERKLM
Sbjct: 61  LPAHADRDTFKNTLPVISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGERKLM 120

Query: 121 PTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSEAKTPSGLLARPVLTSYYK 180
           PTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSEAKTPSGLLARPVLTSYYK
Sbjct: 121 PTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSEAKTPSGLLARPVLTSYYK 180

Query: 181 SPHFKHRPLDPYTNYTSPNEAILCPDAYQSMYAQLLAGLHHRAAVLRVGAVFASGFIRAL 240
           SPHFKHRPLDPYTNYTSPNEAILCPD++QSMY Q+L GL  R  VLR+GAVFASGFIRAL
Sbjct: 181 SPHFKHRPLDPYTNYTSPNEAILCPDSFQSMYTQMLCGLLEREQVLRLGAVFASGFIRAL 240

Query: 241 RFLQNHFPLLCHDLRTGTLNPQITDHPLRESLRRILLKPDPKLADLVESECSKKSWQGII 300
           RFLQNHFPLLCHDLRTGTLNPQITDHPLRESLRRILLKPDPKLADLVESECSKKSWQGII
Sbjct: 241 RFLQNHFPLLCHDLRTGTLNPQITDHPLRESLRRILLKPDPKLADLVESECSKKSWQGII 300

Query: 301 TRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVS 360
           TRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVS
Sbjct: 301 TRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVS 360

Query: 361 YTLIPTMAYFEFLPVDRNMSLNDNDLHRQLVDLADVRLGHDYELVVTTYAGLYRYRVGDV 420
           YTLIPTMAYFEFLPVDRNMSLNDNDLHRQLVDLADVRLGHDYELVVTTYAGLYRYRVGDV
Sbjct: 361 YTLIPTMAYFEFLPVDRNMSLNDNDLHRQLVDLADVRLGHDYELVVTTYAGLYRYRVGDV 420

Query: 421 LRVAGFKNNAPQFNFICRKNVVLSIDSDKTDEVELQNAMKNAVSQHLMPFDARVSEYTSC 480
           LRVAGFKNNAPQFNFICRKNVVLSIDSDKTDEVELQNAMKNAVSQHLMPFDARVSEYTSC
Sbjct: 421 LRVAGFKNNAPQFNFICRKNVVLSIDSDKTDEVELQNAMKNAVSQHLMPFDARVSEYTSC 480

Query: 481 VSTSKIPGHYVMYWELTVNGATPIPPSIFEDCCLTIEESLNSVYRQGRVSDKSIGALEIK 540
           VSTSKIPGHYVMYWELTVNGATPIPPSIFEDCCLTIEESLNSVYRQGRVSDKSIGALEIK
Sbjct: 481 VSTSKIPGHYVMYWELTVNGATPIPPSIFEDCCLTIEESLNSVYRQGRVSDKSIGALEIK 540

Query: 541 VVEAGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVPGHK 600
           VVEAGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVPGHK
Sbjct: 541 VVEAGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVPGHK 600

Query: 601 QWN 603
           QWN
Sbjct: 601 QWN 603

[Close]

BLAST of MC11g_new0199 vs. NCBI nr
Match: KAG6592005.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1078 bits (2788), Expect = 0.0
Identity = 529/608 (87.01%), Postives = 565/608 (92.93%), Query Frame = 0


Query: 1   MPEEAPPNTN---TTDHYKLLEKNKKSLQFIEDVTANADQVQRRVLAEILSRNAGVEYLR 60
           MPEEAP  ++   TT++YKLL++NKK+LQFIEDVTANA QVQRRVLAEILSRNA VEYLR
Sbjct: 1   MPEEAPQKSSFLSTTEYYKLLQRNKKTLQFIEDVTANAAQVQRRVLAEILSRNAHVEYLR 60

Query: 61  RHGLPAHADRDTFKNTLPVISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGER 120
           RHGL    D  TFKNT+PVISYEDIQ DINRIANGDSSPILCSSPISEFLTSSGTSGGER
Sbjct: 61  RHGLAGATDPKTFKNTIPVISYEDIQHDINRIANGDSSPILCSSPISEFLTSSGTSGGER 120

Query: 121 KLMPTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSEAKTPSGLLARPVLTS 180
           KLMPTIEEELERRSLLYSLLMPVMSQF+GGLEKGKGMYFLF+KSE+KTPSGLLARPVLTS
Sbjct: 121 KLMPTIEEELERRSLLYSLLMPVMSQFMGGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180

Query: 181 YYKSPHFKHRPLDPYTNYTSPNEAILCPDAYQSMYAQLLAGLHHRAAVLRVGAVFASGFI 240
           YYKS HFKHRP DPYTNYTSPNEAILC DAYQSMYAQLL GLHHR  VLRVGAVFASGFI
Sbjct: 181 YYKSAHFKHRPFDPYTNYTSPNEAILCSDAYQSMYAQLLCGLHHRTDVLRVGAVFASGFI 240

Query: 241 RALRFLQNHFPLLCHDLRTGTLNPQITDHPLRESLRRILLKPDPKLADLVESECSKKSWQ 300
           RA+RFL+ HF LLCHD+RTGTLNPQITD P+RESL++ L KPDPKLAD +E ECSKKSW 
Sbjct: 241 RAIRFLEKHFHLLCHDIRTGTLNPQITDQPIRESLKKTL-KPDPKLADFIEGECSKKSWH 300

Query: 301 GIITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPS 360
           GIITR+WPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNP+CKP+
Sbjct: 301 GIITRIWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPICKPA 360

Query: 361 EVSYTLIPTMAYFEFLPVDRNMSLNDNDLHRQLVDLADVRLGHDYELVVTTYAGLYRYRV 420
           EVSYTLIPTMAYFEFLPV+RNMSL + +L  +LVDL DV+LG +YELVVTTYAGLYRYRV
Sbjct: 361 EVSYTLIPTMAYFEFLPVNRNMSLTEKELQLELVDLVDVQLGQEYELVVTTYAGLYRYRV 420

Query: 421 GDVLRVAGFKNNAPQFNFICRKNVVLSIDSDKTDEVELQNAMKNAVSQHLMPFDARVSEY 480
           GDVLRVAGFKNNAPQFNFICRKNV LSIDSDKTDEVELQNA+KNA+S HL+PFDA VSEY
Sbjct: 421 GDVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALS-HLVPFDATVSEY 480

Query: 481 TSCVSTSKIPGHYVMYWELTVNGA--TPIPPSIFEDCCLTIEESLNSVYRQGRVSDKSIG 540
           TSC  TSKIPGHYV+YWEL+VNGA  TPIPPS+FEDCCLTIEESLN+VYRQGRVSDKSIG
Sbjct: 481 TSCADTSKIPGHYVLYWELSVNGAKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIG 540

Query: 541 ALEIKVVEAGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKW 600
            LEIK+VE GTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKW
Sbjct: 541 PLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKW 600

Query: 601 VPGHKQWN 603
           VPGHKQW+
Sbjct: 601 VPGHKQWS 606

[Close]

BLAST of MC11g_new0199 vs. NCBI nr
Match: KAG7024880.1 (Indole-3-acetic acid-amido synthetase GH3.6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1077 bits (2785), Expect = 0.0
Identity = 528/608 (86.84%), Postives = 565/608 (92.93%), Query Frame = 0


Query: 1   MPEEAPPNTN---TTDHYKLLEKNKKSLQFIEDVTANADQVQRRVLAEILSRNAGVEYLR 60
           MPEEAP  ++   TT++YKLL++NKK+LQFIEDVTANA QVQRRVLAEILSRNA VEYLR
Sbjct: 1   MPEEAPQKSSFLSTTEYYKLLQRNKKTLQFIEDVTANAAQVQRRVLAEILSRNAHVEYLR 60

Query: 61  RHGLPAHADRDTFKNTLPVISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGER 120
           RHGL    D  TFKNT+PVISYEDIQ DINRIANGDSSPILCSSPISEFLTSSGTSGGER
Sbjct: 61  RHGLAGATDPKTFKNTIPVISYEDIQHDINRIANGDSSPILCSSPISEFLTSSGTSGGER 120

Query: 121 KLMPTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSEAKTPSGLLARPVLTS 180
           KLMPTIEEELERRSLLYSLLMPVM+QF+GGLEKGKGMYFLF+KSE+KTPSGLLARPVLTS
Sbjct: 121 KLMPTIEEELERRSLLYSLLMPVMNQFMGGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180

Query: 181 YYKSPHFKHRPLDPYTNYTSPNEAILCPDAYQSMYAQLLAGLHHRAAVLRVGAVFASGFI 240
           YYKS HFKHRP DPYTNYTSPNEAILC DAYQSMYAQLL GLHHR  VLRVGAVFASGFI
Sbjct: 181 YYKSAHFKHRPFDPYTNYTSPNEAILCSDAYQSMYAQLLCGLHHRTDVLRVGAVFASGFI 240

Query: 241 RALRFLQNHFPLLCHDLRTGTLNPQITDHPLRESLRRILLKPDPKLADLVESECSKKSWQ 300
           RA+RFL+ HF LLCHD+RTGTLNPQITD P+RESL++ L KPDPKLAD +E ECSKKSW 
Sbjct: 241 RAIRFLEKHFHLLCHDIRTGTLNPQITDQPIRESLKKTL-KPDPKLADFIEGECSKKSWH 300

Query: 301 GIITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPS 360
           GIITR+WPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNP+CKP+
Sbjct: 301 GIITRIWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPICKPA 360

Query: 361 EVSYTLIPTMAYFEFLPVDRNMSLNDNDLHRQLVDLADVRLGHDYELVVTTYAGLYRYRV 420
           EVSYTLIPTMAYFEFLPV+RNMSL + +L  +LVDL DV+LG +YELVVTTYAGLYRYRV
Sbjct: 361 EVSYTLIPTMAYFEFLPVNRNMSLTEKELQLELVDLVDVQLGQEYELVVTTYAGLYRYRV 420

Query: 421 GDVLRVAGFKNNAPQFNFICRKNVVLSIDSDKTDEVELQNAMKNAVSQHLMPFDARVSEY 480
           GDVLRVAGFKNNAPQFNFICRKNV LSIDSDKTDEVELQNA+KNA+S HL+PFDA VSEY
Sbjct: 421 GDVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALS-HLVPFDATVSEY 480

Query: 481 TSCVSTSKIPGHYVMYWELTVNGA--TPIPPSIFEDCCLTIEESLNSVYRQGRVSDKSIG 540
           TSC  TSKIPGHYV+YWEL+VNGA  TPIPPS+FEDCCLTIEESLN+VYRQGRVSDKSIG
Sbjct: 481 TSCADTSKIPGHYVLYWELSVNGAKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIG 540

Query: 541 ALEIKVVEAGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKW 600
            LEIK+VE GTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKW
Sbjct: 541 PLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKW 600

Query: 601 VPGHKQWN 603
           VPGHKQW+
Sbjct: 601 VPGHKQWS 606

[Close]

BLAST of MC11g_new0199 vs. NCBI nr
Match: XP_022935867.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 1077 bits (2785), Expect = 0.0
Identity = 529/608 (87.01%), Postives = 564/608 (92.76%), Query Frame = 0


Query: 1   MPEEAPPNTN---TTDHYKLLEKNKKSLQFIEDVTANADQVQRRVLAEILSRNAGVEYLR 60
           MPEEAP  ++   TT++YKLL++NKK+LQFIEDVTANA QVQRRVLAEILSRNA VEYLR
Sbjct: 1   MPEEAPQKSSFLSTTEYYKLLQRNKKTLQFIEDVTANAAQVQRRVLAEILSRNAHVEYLR 60

Query: 61  RHGLPAHADRDTFKNTLPVISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGER 120
           RHGL    D  TFK T+PVISYEDIQ DINRIANGDSSPILCSSPISEFLTSSGTSGGER
Sbjct: 61  RHGLAGATDPKTFKKTIPVISYEDIQHDINRIANGDSSPILCSSPISEFLTSSGTSGGER 120

Query: 121 KLMPTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSEAKTPSGLLARPVLTS 180
           KLMPTIEEELERRSLLYSLLMPVMSQF+GGLEKGKGMYFLF+KSE+KTPSGLLARPVLTS
Sbjct: 121 KLMPTIEEELERRSLLYSLLMPVMSQFMGGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180

Query: 181 YYKSPHFKHRPLDPYTNYTSPNEAILCPDAYQSMYAQLLAGLHHRAAVLRVGAVFASGFI 240
           YYKS HFKHRP DPYTNYTSPNEAILC DAYQSMYAQLL GLHHR  VLRVGAVFASGFI
Sbjct: 181 YYKSAHFKHRPFDPYTNYTSPNEAILCSDAYQSMYAQLLCGLHHRTDVLRVGAVFASGFI 240

Query: 241 RALRFLQNHFPLLCHDLRTGTLNPQITDHPLRESLRRILLKPDPKLADLVESECSKKSWQ 300
           RA+RFL+ HF LLCHD+RTGTLNPQITD P+RESL++ L KPDPKLAD +E ECSKKSW 
Sbjct: 241 RAIRFLEKHFHLLCHDIRTGTLNPQITDQPIRESLKKTL-KPDPKLADFIEGECSKKSWH 300

Query: 301 GIITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPS 360
           GIITR+WPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNP+CKPS
Sbjct: 301 GIITRIWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPICKPS 360

Query: 361 EVSYTLIPTMAYFEFLPVDRNMSLNDNDLHRQLVDLADVRLGHDYELVVTTYAGLYRYRV 420
           EVSYTLIPTMAYFEFLPV+RNMSL + +L  +LVDL DV+LG +YELVVTTYAGLYRYRV
Sbjct: 361 EVSYTLIPTMAYFEFLPVNRNMSLTEKELQLELVDLVDVQLGQEYELVVTTYAGLYRYRV 420

Query: 421 GDVLRVAGFKNNAPQFNFICRKNVVLSIDSDKTDEVELQNAMKNAVSQHLMPFDARVSEY 480
           GDVLRVAGFKNNAPQFNFICRKNV LSIDSDKTDEVELQNA+KNA+S HL+PFDA VSEY
Sbjct: 421 GDVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALS-HLVPFDATVSEY 480

Query: 481 TSCVSTSKIPGHYVMYWELTVNGA--TPIPPSIFEDCCLTIEESLNSVYRQGRVSDKSIG 540
           TSC  TSKIPGHYV+YWEL+VNGA  TPIPPS+FEDCCLTIEESLN+VYRQGRVSDKSIG
Sbjct: 481 TSCADTSKIPGHYVLYWELSVNGAKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIG 540

Query: 541 ALEIKVVEAGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKW 600
            LEIK+VE GTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKW
Sbjct: 541 PLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKW 600

Query: 601 VPGHKQWN 603
           VPGHKQW+
Sbjct: 601 VPGHKQWS 606

[Close]

BLAST of MC11g_new0199 vs. NCBI nr
Match: XP_023535071.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1075 bits (2781), Expect = 0.0
Identity = 527/608 (86.68%), Postives = 564/608 (92.76%), Query Frame = 0


Query: 1   MPEEAPPNTN---TTDHYKLLEKNKKSLQFIEDVTANADQVQRRVLAEILSRNAGVEYLR 60
           MPEEAP  ++   TT++YKLL++NKK+LQFIEDVTANA QVQRRVLAEILSRNA VEYLR
Sbjct: 1   MPEEAPQKSSFLSTTEYYKLLQRNKKTLQFIEDVTANAAQVQRRVLAEILSRNAHVEYLR 60

Query: 61  RHGLPAHADRDTFKNTLPVISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGER 120
           RH L    D  TFKNT+PVISYEDIQ DINRIANGDSSPILCSSPISEFLTSSGTSGGER
Sbjct: 61  RHSLAGATDPKTFKNTIPVISYEDIQHDINRIANGDSSPILCSSPISEFLTSSGTSGGER 120

Query: 121 KLMPTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSEAKTPSGLLARPVLTS 180
           KLMPTIEEELERRSLLYSLLMPVMSQF+GGLEKGKGMYFLF+KSE+KTPSGLLARPVLTS
Sbjct: 121 KLMPTIEEELERRSLLYSLLMPVMSQFMGGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180

Query: 181 YYKSPHFKHRPLDPYTNYTSPNEAILCPDAYQSMYAQLLAGLHHRAAVLRVGAVFASGFI 240
           YYKS HFKHRP DPYTNYTSPNEAILC DAYQSMYAQLL GLHHR  VLRVGAVFASGFI
Sbjct: 181 YYKSAHFKHRPFDPYTNYTSPNEAILCSDAYQSMYAQLLCGLHHRTDVLRVGAVFASGFI 240

Query: 241 RALRFLQNHFPLLCHDLRTGTLNPQITDHPLRESLRRILLKPDPKLADLVESECSKKSWQ 300
           RA+RFL+ HF LLCHD+RTGTLNPQITD P+RESL++ L KPDPKLAD +E ECSKKSW 
Sbjct: 241 RAIRFLEKHFHLLCHDIRTGTLNPQITDQPIRESLKKTL-KPDPKLADFIEGECSKKSWH 300

Query: 301 GIITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPS 360
           GIITR+WPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNP+CKP+
Sbjct: 301 GIITRIWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPICKPA 360

Query: 361 EVSYTLIPTMAYFEFLPVDRNMSLNDNDLHRQLVDLADVRLGHDYELVVTTYAGLYRYRV 420
           EVSYTLIPTMAYFEFLPV+RNMSL + +L  +LVDL DV+LG +YELVVTTYAGLYRYRV
Sbjct: 361 EVSYTLIPTMAYFEFLPVNRNMSLTEKELQLELVDLVDVQLGQEYELVVTTYAGLYRYRV 420

Query: 421 GDVLRVAGFKNNAPQFNFICRKNVVLSIDSDKTDEVELQNAMKNAVSQHLMPFDARVSEY 480
           GDVLRVAGFKNNAPQFNFICRKNV LSIDSDKTDEVELQNA+KNA+S HL+PFDA VSEY
Sbjct: 421 GDVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALS-HLVPFDATVSEY 480

Query: 481 TSCVSTSKIPGHYVMYWELTVNGA--TPIPPSIFEDCCLTIEESLNSVYRQGRVSDKSIG 540
           TSC  TSKIPGHYV+YWEL+VNGA  TPIPPS+FEDCCLTIEESLN+VYRQGRVSDKSIG
Sbjct: 481 TSCADTSKIPGHYVLYWELSVNGAKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIG 540

Query: 541 ALEIKVVEAGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKW 600
            LEIK+VE GTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSR+VSNYFSPKCPKW
Sbjct: 541 PLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRIVSNYFSPKCPKW 600

Query: 601 VPGHKQWN 603
           VPGHKQW+
Sbjct: 601 VPGHKQWS 606

[Close]

BLAST of MC11g_new0199 vs. ExPASy TrEMBL
Match: A0A6J1CDU3 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 GN=LOC111010333 PE=3 SV=1)

HSP 1 Score: 1210 bits (3131), Expect = 0.0
Identity = 593/603 (98.34%), Postives = 597/603 (99.00%), Query Frame = 0


Query: 1   MPEEAPPNTNTTDHYKLLEKNKKSLQFIEDVTANADQVQRRVLAEILSRNAGVEYLRRHG 60
           MPEEAPPNTNTTDHYKLLEKNKKSLQFIEDVTANADQVQRRVLAEILSRNAGVEYLRRHG
Sbjct: 1   MPEEAPPNTNTTDHYKLLEKNKKSLQFIEDVTANADQVQRRVLAEILSRNAGVEYLRRHG 60

Query: 61  LPAHADRDTFKNTLPVISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGERKLM 120
           LPAHADRDTFKNTLPVISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGERKLM
Sbjct: 61  LPAHADRDTFKNTLPVISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGERKLM 120

Query: 121 PTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSEAKTPSGLLARPVLTSYYK 180
           PTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSEAKTPSGLLARPVLTSYYK
Sbjct: 121 PTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSEAKTPSGLLARPVLTSYYK 180

Query: 181 SPHFKHRPLDPYTNYTSPNEAILCPDAYQSMYAQLLAGLHHRAAVLRVGAVFASGFIRAL 240
           SPHFKHRPLDPYTNYTSPNEAILCPD++QSMY Q+L GL  R  VLR+GAVFASGFIRAL
Sbjct: 181 SPHFKHRPLDPYTNYTSPNEAILCPDSFQSMYTQMLCGLLEREQVLRLGAVFASGFIRAL 240

Query: 241 RFLQNHFPLLCHDLRTGTLNPQITDHPLRESLRRILLKPDPKLADLVESECSKKSWQGII 300
           RFLQNHFPLLCHDLRTGTLNPQITDHPLRESLRRILLKPDPKLADLVESECSKKSWQGII
Sbjct: 241 RFLQNHFPLLCHDLRTGTLNPQITDHPLRESLRRILLKPDPKLADLVESECSKKSWQGII 300

Query: 301 TRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVS 360
           TRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVS
Sbjct: 301 TRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVS 360

Query: 361 YTLIPTMAYFEFLPVDRNMSLNDNDLHRQLVDLADVRLGHDYELVVTTYAGLYRYRVGDV 420
           YTLIPTMAYFEFLPVDRNMSLNDNDLHRQLVDLADVRLGHDYELVVTTYAGLYRYRVGDV
Sbjct: 361 YTLIPTMAYFEFLPVDRNMSLNDNDLHRQLVDLADVRLGHDYELVVTTYAGLYRYRVGDV 420

Query: 421 LRVAGFKNNAPQFNFICRKNVVLSIDSDKTDEVELQNAMKNAVSQHLMPFDARVSEYTSC 480
           LRVAGFKNNAPQFNFICRKNVVLSIDSDKTDEVELQNAMKNAVSQHLMPFDARVSEYTSC
Sbjct: 421 LRVAGFKNNAPQFNFICRKNVVLSIDSDKTDEVELQNAMKNAVSQHLMPFDARVSEYTSC 480

Query: 481 VSTSKIPGHYVMYWELTVNGATPIPPSIFEDCCLTIEESLNSVYRQGRVSDKSIGALEIK 540
           VSTSKIPGHYVMYWELTVNGATPIPPSIFEDCCLTIEESLNSVYRQGRVSDKSIGALEIK
Sbjct: 481 VSTSKIPGHYVMYWELTVNGATPIPPSIFEDCCLTIEESLNSVYRQGRVSDKSIGALEIK 540

Query: 541 VVEAGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVPGHK 600
           VVEAGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVPGHK
Sbjct: 541 VVEAGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVPGHK 600

Query: 601 QWN 603
           QWN
Sbjct: 601 QWN 603

[Close]

BLAST of MC11g_new0199 vs. ExPASy TrEMBL
Match: A0A6J1F6T2 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111442645 PE=3 SV=1)

HSP 1 Score: 1077 bits (2785), Expect = 0.0
Identity = 529/608 (87.01%), Postives = 564/608 (92.76%), Query Frame = 0


Query: 1   MPEEAPPNTN---TTDHYKLLEKNKKSLQFIEDVTANADQVQRRVLAEILSRNAGVEYLR 60
           MPEEAP  ++   TT++YKLL++NKK+LQFIEDVTANA QVQRRVLAEILSRNA VEYLR
Sbjct: 1   MPEEAPQKSSFLSTTEYYKLLQRNKKTLQFIEDVTANAAQVQRRVLAEILSRNAHVEYLR 60

Query: 61  RHGLPAHADRDTFKNTLPVISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGER 120
           RHGL    D  TFK T+PVISYEDIQ DINRIANGDSSPILCSSPISEFLTSSGTSGGER
Sbjct: 61  RHGLAGATDPKTFKKTIPVISYEDIQHDINRIANGDSSPILCSSPISEFLTSSGTSGGER 120

Query: 121 KLMPTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSEAKTPSGLLARPVLTS 180
           KLMPTIEEELERRSLLYSLLMPVMSQF+GGLEKGKGMYFLF+KSE+KTPSGLLARPVLTS
Sbjct: 121 KLMPTIEEELERRSLLYSLLMPVMSQFMGGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180

Query: 181 YYKSPHFKHRPLDPYTNYTSPNEAILCPDAYQSMYAQLLAGLHHRAAVLRVGAVFASGFI 240
           YYKS HFKHRP DPYTNYTSPNEAILC DAYQSMYAQLL GLHHR  VLRVGAVFASGFI
Sbjct: 181 YYKSAHFKHRPFDPYTNYTSPNEAILCSDAYQSMYAQLLCGLHHRTDVLRVGAVFASGFI 240

Query: 241 RALRFLQNHFPLLCHDLRTGTLNPQITDHPLRESLRRILLKPDPKLADLVESECSKKSWQ 300
           RA+RFL+ HF LLCHD+RTGTLNPQITD P+RESL++ L KPDPKLAD +E ECSKKSW 
Sbjct: 241 RAIRFLEKHFHLLCHDIRTGTLNPQITDQPIRESLKKTL-KPDPKLADFIEGECSKKSWH 300

Query: 301 GIITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPS 360
           GIITR+WPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNP+CKPS
Sbjct: 301 GIITRIWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPICKPS 360

Query: 361 EVSYTLIPTMAYFEFLPVDRNMSLNDNDLHRQLVDLADVRLGHDYELVVTTYAGLYRYRV 420
           EVSYTLIPTMAYFEFLPV+RNMSL + +L  +LVDL DV+LG +YELVVTTYAGLYRYRV
Sbjct: 361 EVSYTLIPTMAYFEFLPVNRNMSLTEKELQLELVDLVDVQLGQEYELVVTTYAGLYRYRV 420

Query: 421 GDVLRVAGFKNNAPQFNFICRKNVVLSIDSDKTDEVELQNAMKNAVSQHLMPFDARVSEY 480
           GDVLRVAGFKNNAPQFNFICRKNV LSIDSDKTDEVELQNA+KNA+S HL+PFDA VSEY
Sbjct: 421 GDVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALS-HLVPFDATVSEY 480

Query: 481 TSCVSTSKIPGHYVMYWELTVNGA--TPIPPSIFEDCCLTIEESLNSVYRQGRVSDKSIG 540
           TSC  TSKIPGHYV+YWEL+VNGA  TPIPPS+FEDCCLTIEESLN+VYRQGRVSDKSIG
Sbjct: 481 TSCADTSKIPGHYVLYWELSVNGAKTTPIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIG 540

Query: 541 ALEIKVVEAGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKW 600
            LEIK+VE GTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKW
Sbjct: 541 PLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKW 600

Query: 601 VPGHKQWN 603
           VPGHKQW+
Sbjct: 601 VPGHKQWS 606

[Close]

BLAST of MC11g_new0199 vs. ExPASy TrEMBL
Match: A0A6J1IM75 (indole-3-acetic acid-amido synthetase GH3.6 OS=Cucurbita maxima OX=3661 GN=LOC111476851 PE=3 SV=1)

HSP 1 Score: 1070 bits (2768), Expect = 0.0
Identity = 525/608 (86.35%), Postives = 562/608 (92.43%), Query Frame = 0


Query: 1   MPEEAPPNTN---TTDHYKLLEKNKKSLQFIEDVTANADQVQRRVLAEILSRNAGVEYLR 60
           MPEEAP  ++   TT++YKLL++NKK+LQFIEDVTANA Q+QRRVLAEILSRNA VEYLR
Sbjct: 1   MPEEAPQKSSFLSTTEYYKLLQRNKKTLQFIEDVTANAAQIQRRVLAEILSRNAHVEYLR 60

Query: 61  RHGLPAHADRDTFKNTLPVISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGER 120
           RHGL    D  TFKNT+PVISYEDIQ DINRIANGD SPILCSSPISEFLTSSGTSGGER
Sbjct: 61  RHGLAGATDSKTFKNTIPVISYEDIQHDINRIANGDFSPILCSSPISEFLTSSGTSGGER 120

Query: 121 KLMPTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSEAKTPSGLLARPVLTS 180
           KLMPTIEEELERRSLLYSLLMPVMSQF+GGLEKGKGMYFLF+KSE+KTPSGLLARPVLTS
Sbjct: 121 KLMPTIEEELERRSLLYSLLMPVMSQFMGGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180

Query: 181 YYKSPHFKHRPLDPYTNYTSPNEAILCPDAYQSMYAQLLAGLHHRAAVLRVGAVFASGFI 240
           YYKS HFKHRP DPYTNYTSPNEAILC DAYQSMYAQLL GLHHR  VLRVGAVFASGFI
Sbjct: 181 YYKSAHFKHRPFDPYTNYTSPNEAILCSDAYQSMYAQLLCGLHHRTDVLRVGAVFASGFI 240

Query: 241 RALRFLQNHFPLLCHDLRTGTLNPQITDHPLRESLRRILLKPDPKLADLVESECSKKSWQ 300
           RA+RFL+ HF LLCHD+RTGTLNPQITD P+RESL++ L KPDPKLAD +E ECSKKSW 
Sbjct: 241 RAIRFLEKHFHLLCHDIRTGTLNPQITDQPIRESLKKTL-KPDPKLADFIEGECSKKSWH 300

Query: 301 GIITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPS 360
           GIITR+WPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNP+CKP+
Sbjct: 301 GIITRIWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPICKPA 360

Query: 361 EVSYTLIPTMAYFEFLPVDRNMSLNDNDLHRQLVDLADVRLGHDYELVVTTYAGLYRYRV 420
           EVSYTLIPTMAYFEFLPV+RNMSL + +L  +LVDL DV+LG +YELVVTTYAGLYRYRV
Sbjct: 361 EVSYTLIPTMAYFEFLPVNRNMSLTEKELQLELVDLVDVQLGQEYELVVTTYAGLYRYRV 420

Query: 421 GDVLRVAGFKNNAPQFNFICRKNVVLSIDSDKTDEVELQNAMKNAVSQHLMPFDARVSEY 480
           GDVLRVAGFKNNAPQFNFICRKNV LSIDSDKTDEVELQNA+KNA+S HL+PFDA VSEY
Sbjct: 421 GDVLRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALS-HLVPFDATVSEY 480

Query: 481 TSCVSTSKIPGHYVMYWELTVNGA--TPIPPSIFEDCCLTIEESLNSVYRQGRVSDKSIG 540
           TSC  TSKIPGHYV+YWEL+VNGA  T IPPS+FEDCCLTIEESLN+VYRQGRVSDKSIG
Sbjct: 481 TSCADTSKIPGHYVLYWELSVNGAKTTLIPPSVFEDCCLTIEESLNAVYRQGRVSDKSIG 540

Query: 541 ALEIKVVEAGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKW 600
            LEIK+VE GTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSR VSNYFSPKCPKW
Sbjct: 541 PLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRAVSNYFSPKCPKW 600

Query: 601 VPGHKQWN 603
           VPGHKQW+
Sbjct: 601 VPGHKQWS 606

[Close]

BLAST of MC11g_new0199 vs. ExPASy TrEMBL
Match: A0A5A7V8A5 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold977G00060 PE=3 SV=1)

HSP 1 Score: 1015 bits (2625), Expect = 0.0
Identity = 501/608 (82.40%), Postives = 546/608 (89.80%), Query Frame = 0


Query: 1   MPEEAPPNTN---TTDHYKLLEKNKKSLQFIEDVTANADQVQRRVLAEILSRNAGVEYLR 60
           MPEE P  ++   TT++YKLLEKNKK+LQFIEDVT NADQVQRRVL EILSRNAGVEYLR
Sbjct: 1   MPEETPQKSSFLSTTEYYKLLEKNKKTLQFIEDVTTNADQVQRRVLTEILSRNAGVEYLR 60

Query: 61  RHGLPAHADRDTFKNTLPVISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGER 120
           RHGL    D  TFKNTLP+ISYEDIQPDINRIANGD+SPILCSSPISEFLTSSGTSGGER
Sbjct: 61  RHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDTSPILCSSPISEFLTSSGTSGGER 120

Query: 121 KLMPTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSEAKTPSGLLARPVLTS 180
           KLMPTIEEELERRS LYSLLMPVMSQF+ GLEKGKGMYFLF+KSE+KTPSGLLARPVLTS
Sbjct: 121 KLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180

Query: 181 YYKSPHFKHRPLDPYTNYTSPNEAILCPDAYQSMYAQLLAGLHHRAAVLRVGAVFASGFI 240
           YYKSPHFK+RP DPYTNYTSPNEAILC D+YQSMYAQLL GL HR  VLRVGAVFASGFI
Sbjct: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFI 240

Query: 241 RALRFLQNHFPLLCHDLRTGTLNPQITDHPLRESLRRILLKPDPKLADLVESECSKKSWQ 300
           RA+RFL+ H+ LLCHD+RTGTLN +ITD  +RES+R+IL K D KLAD VE EC KK W 
Sbjct: 241 RAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKIL-KADGKLADFVEGECGKKCWG 300

Query: 301 GIITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPS 360
           GII+R+WPNTKYVDVIVTGTMSQYI TLDYYSNGLP+VCTMYASSECYFGVNL P+CKP 
Sbjct: 301 GIISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPG 360

Query: 361 EVSYTLIPTMAYFEFLPVDRNMSLNDNDLHRQLVDLADVRLGHDYELVVTTYAGLYRYRV 420
           EV+YTLIPTMAYFEFLPV+R+    + +L ++LVDL DV+LGH+YELVVTTY+GLYRYRV
Sbjct: 361 EVAYTLIPTMAYFEFLPVNRS---KEKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRV 420

Query: 421 GDVLRVAGFKNNAPQFNFICRKNVVLSIDSDKTDEVELQNAMKNAVSQHLMPFDARVSEY 480
           GD+LRVAGFKNNAPQFNFICRKNV LSIDSDKTDEVELQNA+KNA++ HL+PFD  +SEY
Sbjct: 421 GDILRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALT-HLIPFDVTLSEY 480

Query: 481 TSCVSTSKIPGHYVMYWELTVNG---ATPIPPSIFEDCCLTIEESLNSVYRQGRVSDKSI 540
           TSCV TSKIPGHYV+YWELT N    +TPIPPS+FEDCC  IEESLN+VYRQGRVSDKSI
Sbjct: 481 TSCVDTSKIPGHYVLYWELTPNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSI 540

Query: 541 GALEIKVVEAGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPK 600
           G LEIK+VE GTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSR VSNYFSPKCPK
Sbjct: 541 GPLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRTVSNYFSPKCPK 600

Query: 601 WVPGHKQW 602
           WVP  KQW
Sbjct: 601 WVP--KQW 601

[Close]

BLAST of MC11g_new0199 vs. ExPASy TrEMBL
Match: A0A1S3BYK9 (indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo OX=3656 GN=LOC103494980 PE=3 SV=1)

HSP 1 Score: 1015 bits (2625), Expect = 0.0
Identity = 501/608 (82.40%), Postives = 546/608 (89.80%), Query Frame = 0


Query: 1   MPEEAPPNTN---TTDHYKLLEKNKKSLQFIEDVTANADQVQRRVLAEILSRNAGVEYLR 60
           MPEE P  ++   TT++YKLLEKNKK+LQFIEDVT NADQVQRRVL EILSRNAGVEYLR
Sbjct: 1   MPEETPQKSSFLSTTEYYKLLEKNKKTLQFIEDVTTNADQVQRRVLTEILSRNAGVEYLR 60

Query: 61  RHGLPAHADRDTFKNTLPVISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGER 120
           RHGL    D  TFKNTLP+ISYEDIQPDINRIANGD+SPILCSSPISEFLTSSGTSGGER
Sbjct: 61  RHGLGGATDCKTFKNTLPLISYEDIQPDINRIANGDTSPILCSSPISEFLTSSGTSGGER 120

Query: 121 KLMPTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSEAKTPSGLLARPVLTS 180
           KLMPTIEEELERRS LYSLLMPVMSQF+ GLEKGKGMYFLF+KSE+KTPSGLLARPVLTS
Sbjct: 121 KLMPTIEEELERRSSLYSLLMPVMSQFIEGLEKGKGMYFLFIKSESKTPSGLLARPVLTS 180

Query: 181 YYKSPHFKHRPLDPYTNYTSPNEAILCPDAYQSMYAQLLAGLHHRAAVLRVGAVFASGFI 240
           YYKSPHFK+RP DPYTNYTSPNEAILC D+YQSMYAQLL GL HR  VLRVGAVFASGFI
Sbjct: 181 YYKSPHFKNRPFDPYTNYTSPNEAILCSDSYQSMYAQLLCGLLHRTDVLRVGAVFASGFI 240

Query: 241 RALRFLQNHFPLLCHDLRTGTLNPQITDHPLRESLRRILLKPDPKLADLVESECSKKSWQ 300
           RA+RFL+ H+ LLCHD+RTGTLN +ITD  +RES+R+IL K D KLAD VE EC KK W 
Sbjct: 241 RAIRFLEKHYTLLCHDIRTGTLNSEITDQGIRESVRKIL-KADGKLADFVEGECGKKCWG 300

Query: 301 GIITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPS 360
           GII+R+WPNTKYVDVIVTGTMSQYI TLDYYSNGLP+VCTMYASSECYFGVNL P+CKP 
Sbjct: 301 GIISRIWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPIVCTMYASSECYFGVNLKPMCKPG 360

Query: 361 EVSYTLIPTMAYFEFLPVDRNMSLNDNDLHRQLVDLADVRLGHDYELVVTTYAGLYRYRV 420
           EV+YTLIPTMAYFEFLPV+R+    + +L ++LVDL DV+LGH+YELVVTTY+GLYRYRV
Sbjct: 361 EVAYTLIPTMAYFEFLPVNRS---KEKELEKELVDLVDVKLGHEYELVVTTYSGLYRYRV 420

Query: 421 GDVLRVAGFKNNAPQFNFICRKNVVLSIDSDKTDEVELQNAMKNAVSQHLMPFDARVSEY 480
           GD+LRVAGFKNNAPQFNFICRKNV LSIDSDKTDEVELQNA+KNA++ HL+PFD  +SEY
Sbjct: 421 GDILRVAGFKNNAPQFNFICRKNVALSIDSDKTDEVELQNAVKNALT-HLIPFDVTLSEY 480

Query: 481 TSCVSTSKIPGHYVMYWELTVNG---ATPIPPSIFEDCCLTIEESLNSVYRQGRVSDKSI 540
           TSCV TSKIPGHYV+YWELT N    +TPIPPS+FEDCC  IEESLN+VYRQGRVSDKSI
Sbjct: 481 TSCVDTSKIPGHYVLYWELTPNNEIKSTPIPPSVFEDCCFAIEESLNAVYRQGRVSDKSI 540

Query: 541 GALEIKVVEAGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPK 600
           G LEIK+VE GTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSR VSNYFSPKCPK
Sbjct: 541 GPLEIKIVETGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRTVSNYFSPKCPK 600

Query: 601 WVPGHKQW 602
           WVP  KQW
Sbjct: 601 WVP--KQW 601

[Close]

BLAST of MC11g_new0199 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 964.1 bits (2491), Expect = 5.1e-281
Identity = 475/611 (77.74%), Postives = 523/611 (85.60%), Query Frame = 0


Query: 1   MPEEAPPNTNTTDHYKLLEKNKKSLQFIEDVTANADQVQRRVLAEILSRNAGVEYLRRHG 60
           MPE             L EKNK  LQFIEDVT NAD VQRRVL EILSRNA VEYL+RHG
Sbjct: 1   MPEAPKIAALEVSDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHG 60

Query: 61  LPAHADRDTFKNTLPVISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGERKLM 120
           L    DR+TFK+ +PV++YEDIQP+INRIANGD S +LCS+PISEFLTSSGTSGGERKLM
Sbjct: 61  LEGRTDRETFKHIMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLM 120

Query: 121 PTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSEAKTPSGLLARPVLTSYYK 180
           PTIEEEL+RRSLLYSLLMPVM QFV GL+KGKGMYFLF+KSE+KTP GL ARPVLTSYYK
Sbjct: 121 PTIEEELDRRSLLYSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYK 180

Query: 181 SPHFKHRPLDPYTNYTSPNEAILCPDAYQSMYAQLLAGLHHRAAVLRVGAVFASGFIRAL 240
           S HFK+RP DPYTNYTSPN+ ILC D+YQSMY+Q+L GL     VLRVGAVFASGFIRA+
Sbjct: 181 SSHFKNRPYDPYTNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAI 240

Query: 241 RFLQNHFPLLCHDLRTGTLNPQITDHPLRESLRRILLKPDPKLADLVESECSKKSWQGII 300
           +FL+ H+P L  D+RTGTL+ +ITD  +RE++  I LKPDPKLAD VESEC K SWQGII
Sbjct: 241 KFLEKHWPELARDIRTGTLSSEITDSSVREAVGEI-LKPDPKLADFVESECRKTSWQGII 300

Query: 301 TRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVS 360
           TRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNL PLCKPSEVS
Sbjct: 301 TRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVS 360

Query: 361 YTLIPTMAYFEFLPVDRNMSLNDN---------DLHRQLVDLADVRLGHDYELVVTTYAG 420
           YTLIP MAYFEFLPV RN  +  +            ++LVDL DV+LG +YELVVTTYAG
Sbjct: 361 YTLIPNMAYFEFLPVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAG 420

Query: 421 LYRYRVGDVLRVAGFKNNAPQFNFICRKNVVLSIDSDKTDEVELQNAMKNAVSQHLMPFD 480
           LYRYRVGDVL VAGFKNNAPQF+FICRKNVVLSIDSDKTDEVELQNA+KNAV+ HL+PFD
Sbjct: 421 LYRYRVGDVLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVT-HLVPFD 480

Query: 481 ARVSEYTSCVSTSKIPGHYVMYWELTVNGATPIPPSIFEDCCLTIEESLNSVYRQGRVSD 540
           A +SEYTS   TS IPGHYV++WEL +NG TPIPPS+FEDCCLTIEESLNSVYRQGRVSD
Sbjct: 481 ASLSEYTSYADTSSIPGHYVLFWELCLNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSD 540

Query: 541 KSIGALEIKVVEAGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPK 600
           KSIG LEIK+VE+GTFDKLMDYAISLGASINQYKTPRCVK+ PII+LLNSRVV +YFSPK
Sbjct: 541 KSIGPLEIKMVESGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPK 600

Query: 601 CPKWVPGHKQW 603
           CPKW PGHKQW
Sbjct: 601 CPKWSPGHKQW 609

[Close]

BLAST of MC11g_new0199 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 938.3 bits (2424), Expect = 3.0e-273
Identity = 464/611 (75.94%), Postives = 518/611 (84.78%), Query Frame = 0


Query: 1   MPEEAPPNTNTTDHYKLLEKNKKSLQFIEDVTANADQVQRRVLAEILSRNAGVEYLRRHG 60
           MPE     +       L +KNK+ LQ IE++T+NADQVQR+VL EIL+RNA VEYLRRH 
Sbjct: 1   MPEAPKKESLEVFDLTLDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHD 60

Query: 61  LPAHADRDTFKNTLPVISYEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGERKLM 120
           L    DR+TFKN +PVI+YEDI+P+INRIANGD SPIL S PISEFLTSSGTSGGERKLM
Sbjct: 61  LNGRTDRETFKNIMPVITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLM 120

Query: 121 PTIEEELERRSLLYSLLMPVMSQFVGGLEKGKGMYFLFVKSEAKTPSGLLARPVLTSYYK 180
           PTIEEEL+RRSLLYSLLMPVMSQFV GLE GKGMYFLF+KSE+KTP GL ARPVLTSYYK
Sbjct: 121 PTIEEELDRRSLLYSLLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYK 180

Query: 181 SPHFKHRPLDPYTNYTSPNEAILCPDAYQSMYAQLLAGLHHRAAVLRVGAVFASGFIRAL 240
           S HFK RP DPYTNYTSPNE ILC D+YQSMY+Q+L GL     VLRVGAVFASGFIRA+
Sbjct: 181 SSHFKERPYDPYTNYTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAI 240

Query: 241 RFLQNHFPLLCHDLRTGTLNPQITDHPLRESLRRILLKPDPKLADLVESECSKKSWQGII 300
           +FL+ H+  L  D+RTGTL+  ITD  +RE++ +I LKP PKLAD VE EC K SWQGII
Sbjct: 241 KFLEKHWIELVRDIRTGTLSSLITDPSVREAVAKI-LKPSPKLADFVEFECKKSSWQGII 300

Query: 301 TRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVS 360
           TRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNL PLCKPSEVS
Sbjct: 301 TRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVS 360

Query: 361 YTLIPTMAYFEFLPVDRNMSLNDN---------DLHRQLVDLADVRLGHDYELVVTTYAG 420
           YTLIP+MAYFEFLPV RN  + ++            ++LVDL DV+LG +YELVVTTYAG
Sbjct: 361 YTLIPSMAYFEFLPVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAG 420

Query: 421 LYRYRVGDVLRVAGFKNNAPQFNFICRKNVVLSIDSDKTDEVELQNAMKNAVSQHLMPFD 480
           L RYRVGD+LRV GFKN APQF+FICRKNVVLSIDSDKTDEVELQNA+KNAV+ HL+PFD
Sbjct: 421 LCRYRVGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVT-HLVPFD 480

Query: 481 ARVSEYTSCVSTSKIPGHYVMYWELTVNGATPIPPSIFEDCCLTIEESLNSVYRQGRVSD 540
           A +SEYTS   TS IPGHYV++WEL ++G TPIPPS+FEDCCL +EES N+VYRQGRVSD
Sbjct: 481 ASLSEYTSYADTSSIPGHYVLFWELCLDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSD 540

Query: 541 KSIGALEIKVVEAGTFDKLMDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPK 600
           KSIG LEIK+VE GTFDKLMDYAISLGASINQYKTPRCVK+ PII+LLNSRVV +YFSPK
Sbjct: 541 KSIGPLEIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPK 600

Query: 601 CPKWVPGHKQW 603
           CPKWVPGHKQW
Sbjct: 601 CPKWVPGHKQW 609

[Close]

BLAST of MC11g_new0199 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 765.4 bits (1975), Expect = 3.5e-221
Identity = 380/584 (65.07%), Postives = 455/584 (77.91%), Query Frame = 0


Query: 19  EKNKKSLQFIEDVTANADQVQRRVLAEILSRNAGVEYLRRHGLPAHADRDTFKNTLPVIS 78
           EK+ K+L+FIE++T NAD VQ  +LAEIL+RNA  EYLRR  L    DRDTFK  +PVI+
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76

Query: 79  YEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSLLM 138
           YED+QP+I RIA+GD SPIL + PISEFLTSSGTS GERKLMPTI+EEL+RR LLYSLLM
Sbjct: 77  YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136

Query: 139 PVMSQFVGGLEKGKGMYFLFVKSEAKTPSGLLARPVLTSYYKSPHFKHRPLDPYTNYTSP 198
           PVM+ +V GL+KGKGMYFLFVKSE KTP GL ARPVLTSYYKS HF+ RP DPY  YTSP
Sbjct: 137 PVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTSP 196

Query: 199 NEAILCPDAYQSMYAQLLAGLHHRAAVLRVGAVFASGFIRALRFLQNHFPLLCHDLRTGT 258
           NEAILCPD++QSMY Q+L GL  R +VLRVGAVFASG +RA+RFLQ H+    HD+  G 
Sbjct: 197 NEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGC 256

Query: 259 LNPQITDHPLRESLRRILLKPDPKLADLVESECSKKSWQGIITRLWPNTKYVDVIVTGTM 318
           L+ +ITD  +R+ +  I LKPDP LA+ +  EC   +W+ IITR+WPNTKY+DVIVTG M
Sbjct: 257 LDSEITDPSIRQCMSGI-LKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAM 316

Query: 319 SQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVSYTLIPTMAYFEFLPVDRN 378
           +QYIPTL+YYS GLP+ CTMYASSECYFG+NLNP+ KPSEVSYT++P MAYFEF+P+   
Sbjct: 317 AQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLGGT 376

Query: 379 MSLNDNDLHRQLVDLADVRLGHDYELVVTTYAGLYRYRVGDVLRVAGFKNNAPQFNFICR 438
                     + V+L DV +G +YELVVTTYAGL RYRVGD+LRV GF N+APQF+F+ R
Sbjct: 377 ----------KAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRR 436

Query: 439 KNVVLSIDSDKTDEVELQNAMKNAVSQHLMPFDARVSEYTSCVSTSKIPGHYVMYWELTV 498
           KNV+LSIDSDKTDE ELQ A++NA S       +RV+EYTS   TS IPGHYV+YWEL V
Sbjct: 437 KNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELLV 496

Query: 499 -NGATPIPPSIFEDCCLTIEESLNSVYRQGRVSDKSIGALEIKVVEAGTFDKLMDYAISL 558
            +GA          CCL +EESLNSVYRQ RV+D S+G LEI+VV  GTF++LMDYAIS 
Sbjct: 497 RDGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAISR 556

Query: 559 GASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVPGHKQ 602
           GASINQYK PRCV + PI++LL+SRVVS +FSP  P W P  ++
Sbjct: 557 GASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERRR 589

[Close]

BLAST of MC11g_new0199 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 745.0 bits (1922), Expect = 4.8e-215
Identity = 370/591 (62.61%), Postives = 459/591 (77.66%), Query Frame = 0


Query: 19  EKNKKSLQFIEDVTANADQVQRRVLAEILSRNAGVEYLRRHGLPAHADRDTFKNTLPVIS 78
           EK+ K+L+FIE++T N D VQ +VL EIL+RN+  EYL+R  L    DR TFK+ +PV++
Sbjct: 18  EKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKSKVPVVT 77

Query: 79  YEDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSLLM 138
           YED++P+I RI+NGD SPIL S PI+EFLTSSGTS GERKLMPTIEE+L+RR LLYSLLM
Sbjct: 78  YEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQLLYSLLM 137

Query: 139 PVMSQFVGGLEKGKGMYFLFVKSEAKTPSGLLARPVLTSYYKSPHFKHRPLDPYTNYTSP 198
           PVM+ +V GL+KGKG+YFLFVKSE+KT  GL ARPVLTSYYKS HFK RP DPY  YTSP
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPYNVYTSP 197

Query: 199 NEAILCPDAYQSMYAQLLAGLHHRAAVLRVGAVFASGFIRALRFLQNHFPLLCHDLRTGT 258
           NEAILC D+ QSMYAQ+L GL  R  VLR+GAVFASG +RA+ FLQN++  L  D+ TGT
Sbjct: 198 NEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELARDISTGT 257

Query: 259 LNPQITDHPLRESLRRILLKPDPKLADLVESECSKKSWQGIITRLWPNTKYVDVIVTGTM 318
           L+ +I D  ++  + +IL KPD +LA+ +   CS+++W+GIIT++WPNTKY+DVIVTG M
Sbjct: 258 LSSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLDVIVTGAM 317

Query: 319 SQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVSYTLIPTMAYFEFLPVDRN 378
           +QYIPTL+YYS GLP+ CTMYASSE YFG+NL P+CKPSEVSYT++P MAYFEFLP + +
Sbjct: 318 AQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPHNHD 377

Query: 379 ------MSLNDNDLHRQLVDLADVRLGHDYELVVTTYAGLYRYRVGDVLRVAGFKNNAPQ 438
                  SL++      LV+LA+V +G +YELV+TTYAGLYRYRVGD+LRV GF N+APQ
Sbjct: 378 GDGAAEASLDET----SLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQ 437

Query: 439 FNFICRKNVVLSIDSDKTDEVELQNAMKNAVSQHLMPFDARVSEYTSCVSTSKIPGHYVM 498
           F FI RKNV+LS++SDKTDE ELQ A++NA S+       RV EYTS   T  IPGHYV+
Sbjct: 438 FKFIRRKNVLLSVESDKTDEAELQKAVENA-SRLFAEQGTRVIEYTSYAETKTIPGHYVI 497

Query: 499 YWELTVNGATP--IPPSIFEDCCLTIEESLNSVYRQGRVSDKSIGALEIKVVEAGTFDKL 558
           YWEL     +   +   +   CCL +EESLNSVYRQ RV+DKSIG LEI+VV  GTF++L
Sbjct: 498 YWELLGRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVRNGTFEEL 557

Query: 559 MDYAISLGASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVPGHKQ 602
           MDYAIS GASINQYK PRCV + PI++LL+SRVVS +FSP  P W P  ++
Sbjct: 558 MDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHWSPERRR 603

[Close]

BLAST of MC11g_new0199 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 737.3 bits (1902), Expect = 1.0e-212
Identity = 368/584 (63.01%), Postives = 454/584 (77.74%), Query Frame = 0


Query: 20  KNKKSLQFIEDVTANADQVQRRVLAEILSRNAGVEYLRRHGLPAHADRDTFKNTLPVISY 79
           K+ K+L+FIE++T N D VQ++V+ EILSRN+  EYL+R GL    DR TFK  +PV+ Y
Sbjct: 18  KDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTFKTKVPVVIY 77

Query: 80  EDIQPDINRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSLLMP 139
           +D++P+I RIANGD S IL S PI+EFLTSSGTS GERKLMPTI+E+++RR LLYSLLMP
Sbjct: 78  DDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRRQLLYSLLMP 137

Query: 140 VMSQFVGGLEKGKGMYFLFVKSEAKTPSGLLARPVLTSYYKSPHFKHRPLDPYTNYTSPN 199
           VM+ +V GL+KGK +YFLFVK+E+KTP GL ARPVLTSYYKS  FK RP DPY  YTSPN
Sbjct: 138 VMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPNDPYNVYTSPN 197

Query: 200 EAILCPDAYQSMYAQLLAGLHHRAAVLRVGAVFASGFIRALRFLQNHFPLLCHDLRTGTL 259
           EAILCPD+ QSMY Q+L GL  R  VLR+GAVFASG +RA+ FLQ ++  L  D+ TGTL
Sbjct: 198 EAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELADDISTGTL 257

Query: 260 NPQITDHPLRESLRRILLKPDPKLADLVESECSK-KSWQGIITRLWPNTKYVDVIVTGTM 319
           + +I+D  ++ES+ +IL KPD +LAD + S C +  SW+GIIT++WPNTKY+DVIVTG M
Sbjct: 258 SSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYLDVIVTGAM 317

Query: 320 SQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVSYTLIPTMAYFEFLPVDRN 379
           +QYIP L+YYS GLP+ CTMYASSE YFG+NL P+CKPSEVSYT++P MAYFEFLP    
Sbjct: 318 AQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPHHEV 377

Query: 380 MSLNDNDLHRQLVDLADVRLGHDYELVVTTYAGLYRYRVGDVLRVAGFKNNAPQFNFICR 439
            +        +LV+LADV +G +YELV+TTYAGL RYRVGD+L+V GF N+APQF F+ R
Sbjct: 378 PTEKS-----ELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYNSAPQFKFVRR 437

Query: 440 KNVVLSIDSDKTDEVELQNAMKNAVSQHLMPFDARVSEYTSCVSTSKIPGHYVMYWELTV 499
           KNV+LSI+SDKTDE ELQ+A++NA S  L     RV EYTS   T  IPGHYV+YWEL V
Sbjct: 438 KNVLLSIESDKTDEAELQSAVENA-SLLLGEQGTRVIEYTSYAETKTIPGHYVIYWELLV 497

Query: 500 NGAT-PIPPSIFEDCCLTIEESLNSVYRQGRVSDKSIGALEIKVVEAGTFDKLMDYAISL 559
              T P    +   CCL +EESLNSVYRQ RV+DKSIG LEI+VV+ GTF++LMDYAIS 
Sbjct: 498 KDQTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGTFEELMDYAISR 557

Query: 560 GASINQYKTPRCVKYEPIIQLLNSRVVSNYFSPKCPKWVPGHKQ 602
           GASINQYK PRCV + PI++LL+SRVVS +FSP  P W P  ++
Sbjct: 558 GASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPERRR 595

The following BLAST results are available for this feature:

BLAST of MC11g_new0199 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LSQ4	7.2e-280	77.74	Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
O81829	4.2e-272	75.94	Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q8LQM5	2.7e-239	67.11	Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo...	[more]
Q60EJ6	2.9e-228	66.89	Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japo...	[more]
O82333	4.9e-220	65.07	Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=...	[more]

BLAST of MC11g_new0199 vs. NCBI nr


Match Name	Identity	Description
XP_022139392.1	98.34	indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia]	[more]
KAG6592005.1	87.01	Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so...	[more]
KAG7024880.1	86.84	Indole-3-acetic acid-amido synthetase GH3.6, partial [Cucurbita argyrosperma sub...	[more]
XP_022935867.1	87.01	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata]	[more]
XP_023535071.1	86.68	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC11g_new0199 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1CDU3	98.34	indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 ...	[more]
A0A6J1F6T2	87.01	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1IM75	86.35	indole-3-acetic acid-amido synthetase GH3.6 OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A5A7V8A5	82.40	Indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3BYK9	82.40	indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo OX=3656 GN=LOC103494...	[more]

BLAST of MC11g_new0199 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G54510.1	5.1e-281	77.74	Auxin-responsive GH3 family protein	[more]
AT4G27260.1	3.0e-273	75.94	Auxin-responsive GH3 family protein	[more]
AT2G14960.1	3.5e-221	65.07	Auxin-responsive GH3 family protein	[more]
AT4G37390.1	4.8e-215	62.61	Auxin-responsive GH3 family protein	[more]
AT2G23170.1	1.0e-212	63.01	Auxin-responsive GH3 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004993	GH3 family	PFAM	PF03321	GH3	coord: 28..572 e-value: 2.2E-198 score: 660.4
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 9..602
None	No IPR available	PANTHER	PTHR31901:SF70	BNAC03G14570D PROTEIN	coord: 9..602

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC11g_new0199.1	MC11g_new0199.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0010279	indole-3-acetic acid amido synthetase activity