MC11g_new0166 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC11g_new0166
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionpersulfide dioxygenase ETHE1 homolog, mitochondrial
LocationMC11: 6567722 .. 6571433 (+)
RNA-Seq ExpressionMC11g_new0166
SyntenyMC11g_new0166
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GCTCAAAGGCCAAAGCCAACTACTTTTTTAAGAATGGAATACCTAAGTTGATCACAGGTTACAAATTCACCTTGAATTTTTTTAAGTTTTGTGGACAAAAATTATGGCGTTCACCATGTTTTTCTTGGGCAAGAGGCAAAACCAGGAGCGCGGGATGGAAATGGAGAGGAGAGAGATGACGGATGAGGCGAGGCGAGGCGAAGCTTGCGAAATCTGACACATCTTCCATTGATGACGATTCCGCCATTGTCCCATTTGCCATAAACAAATCCGCTAAATCACTATCATCCTTCGCCACGTTCCAATCCAGTTTGAGTCAATAACAAAGCGCGATGCAAAGGTTTCGATTCTTACGAACTCCTCTGTTTCCTCTCAATCCTCCCTTCTTCGTCTCCCAAACTTCTCATCTTCTTCCGCTGAAACCCATTTCCAAAATTCCCACCAAGAGTATGGCGTCCTCATCATCTTCTTCCTCTTCTTCTAACCTCTTGTTCCGTCAACTCTTCGAGAAGGAATCCTCCACTTTCACCTACCTCCTCGCTGATGTTTCCCATCCGGACAAACCCGCGCTTGTAATTTCCCTTTCTATGTTTTGTTACGGTGTTTGGACTTTGAATTAAATTTGAAATTTGGCTGTTCTTTGCCGAAACGTGTGATGGATTTGGTTTCATTTCATCACAGTTCTTAGTTGGGTGGGGAACTTTGGTCAAAAGCATTAATGGGGTTCTTCGATTTTCTCGTAATTTGTTGAATTTTCTCTGTTCTCAGTTGATTGACCCGGTTGACAAGACTGTAGATAGGGATCTAAACCTTGTTCGAGAACTGGGGCTGAAGCTTGTTTATGCTATGAACACTCATGTGCATGCTGATCATGTCACTGGCACTGGACTTATCAAGGTTATTCTTCTCCATCGTTGTTTTTGTTGGTGATTTTGCATTCCCAATTATCGTTTCTTCTGTGTCGGACTTCATAGTTAATCTTTGATGCGATCATTTCATTTAGAGTAGAGTTCCAGGAGTGGAATCTGTAATCTCGAGGGCAAGTGGTTCAAAAGCTGATGTGTTGATTCAGCCTGGCGATAGAATTCCTTTTGGTGATCTATTCCTGGAGGTAGGCCAATACTTATATTTAACACTACAAAGATACAGTATTGATTTCTGTTTGCTTTCTTAAAGACTAAACAGATATTATCACAATCTGCTATGAATATTTACTTTTGTGGGTCTTGAGAACACAAATGCAATACAGAGACGGAGTCAATTGTTGCACCAGGAGTTGTTCTATATTATTCCACACTGGATCTGGCTCCTTTGTCTCCTATTTTCTTCTTGGGGTGTGGCTCCACATCATTACTTCTTATTGTTGTTTGTTCATTATTCTTATATTACTATCAACAATAATATGCCTTTCTCTGGAAATAAAAAAGTTGTACTGCAAAGATATGTCTTCTAGTTCCCTCTTGATGATCATAACAATCACAATCCTGGAATAGGATTATTTCCCTAGTATAGGTGACCACATTAAGGAAGGAGGAAATAGTTGAACATTTTAGAAACTTTGTGTTATTGCCACCTCCATCTTTCATCTCTTAATGCTGAAATGAAGAAACATATGGATAACATAGGGAGTGTTAGCTAGGAAGCTACCACTTACCATTAGATGTGCCATCATTTTGGTAAAGATTCTGGAGATGCGACTTGGTTTTCTGCTATTATAGAGTTAGAGATCTCTTATGCTTTAATTTGTTTGGAGTGAGAGGGAAATATGTAATTTAACTGTGATTTTACCAAGCCTTGAATGGAAAACTGATGTGTCGTATGGTTTGACGATTTTGATCAACTATGATCCCCACTGTTGTATACTTTCTGGTGTCGGTTCTTTTCCTGAACCCGAGTGACAGCAGAATATTCTTTGTATGCTTTCAAAGGGTTCGTCATCAAGTAAAGCAGTGTTAAGAAAATGATAAATTTTGGGGACAATCCTAGAGTTGACTCTTGACTCGACTGTATCTTGCATTTGATTTCAGGTTCGTGCTACTCCTGGCCATACGTCAGGATGTGTCACCTATGTAACAGGGGAGGAACCTGATCAGCCTCATCCTAGGATGGCATTCACTGGGGATGCTCTGTTGATACGTGGATGTGGCAGGACAGATTTTCAGGTGGCTTTCACACCTTTTGACTTATTGTTCAATTGTTACTGGTAGTACTCGAGTTGTTCTCAAAAGCACTGGTCTTTCAGATAAAGTGATTTTAGTATAATAATTTGGCGGTCTTTTCCCCTCTAGCTTGTGACTCGATGTATATTTAAAAAAATTTCCAAACTTCTTTGGTATCTACTGGGTGGTACACAATTTTCTGTTCTGTTGTATAAACCTCGAGTGATACTGGTAACTGGTGTCGTTTTCATTACACTGGAAACATTATGAATGATCGACTGATCACTTAGGCGTAAATGTTTTCTTAATTAATCACAACCATTCTGCATGATTCTGACCTTTATGTTTAGCACAAACTGCCTTTATCTACAATTTCAACATTTCCAATATAACGTATCGATAAGAAACTTTTCGATATTACTGGATTTCCCGTGCTTGGATAGGTATTATCCTCTTTCACATTTGAAGAATTGACCTTTATTGCTACAAGGCTGAATATCATTGTATTTTCTGCAGGGTGGAAGTTCAAAGCAGCTCTATGAATCTGTACACTCGCAGGTAAGGACCTGATACTTGGTATATTTGCAACTAAAACAGGAAGGAGAGATTATAGGAAAAGCTAAGAACATAATCACTATTAATGGAGTTTCTTTGCTTTAGCTTTCTGTAACATAAACTCATCTTATTATTAACTCTCTGTGCCAGATCTTCACCCTGCCAAAGGACACATTGATCTATCCTGCTCACGATTACAAGGGATTCAATGTAAGGAAAATTTCTACAGTTCATTCTTTCTTCGAAACACGGTTTGGTTACTTATATCCAACTCGGGATTTCAGGTTAGTACTGTAGGCGAAGAGATGGTACACAATCCCCGGCTCACCAAGGATCTGGTAATTTCTTAACTAACCAAAAACACTTATCTGGAAATTTCATTATGAAGCAGAATTAGTTACTAAAACCTGCGTCTAAACTTTGTAACACTTTACCTGGGTTTGCTTTTGTTTTGCAGGAAGAATTCAAGAAAATAATGGAAAGTGAGTACATAAACAAAAATTTTATTCTGGTGTGTACCGATCGCCTCTGCTATAATAAATGTCTGGTTTATCTTTGTTACAGATTTGAACCTGGCGTATCCAAAGATGATTGACATTGCAGTTCCAGCAAATTTGGTTTGTGGTTTGCAAGATCCAGCTTCTTAACTCTGTCAACTCTACGAGTCATTGTGTATAATTGGCCTTCAAATTCAAAATATATAAAATAAAAAAAGAAGAAGAAGAAGAAGAAGAAGAAGAAAGATCAATCAAAATCAATAGGCCAATGGTGTGATCTCCACTAACAAAAATAAAAATACATGTAGAAATGACATTATCTGCTAGCCTTCTGCTATGCCTGTCTTGTGGGTTACAAAATGGTTTGCTTGATGAGTTGTCTCTCTCTTTTCATTTCACTGAAGTTTTTGTTTGCCACCACCCTCAATAAGAATGATTTCTAGTTCATTGATAGATCAAATGACTTGCAAAAATAGTTCTGCAATCTCAATACTGTTCGTA

mRNA sequence

ATGCAAAGGTTTCGATTCTTACGAACTCCTCTGTTTCCTCTCAATCCTCCCTTCTTCGTCTCCCAAACTTCTCATCTTCTTCCGCTGAAACCCATTTCCAAAATTCCCACCAAGAGTATGGCGTCCTCATCATCTTCTTCCTCTTCTTCTAACCTCTTGTTCCGTCAACTCTTCGAGAAGGAATCCTCCACTTTCACCTACCTCCTCGCTGATGTTTCCCATCCGGACAAACCCGCGCTTTTGATTGACCCGGTTGACAAGACTGTAGATAGGGATCTAAACCTTGTTCGAGAACTGGGGCTGAAGCTTGTTTATGCTATGAACACTCATGTGCATGCTGATCATGTCACTGGCACTGGACTTATCAAGAGTAGAGTTCCAGGAGTGGAATCTGTAATCTCGAGGGCAAGTGGTTCAAAAGCTGATGTGTTGATTCAGCCTGGCGATAGAATTCCTTTTGGTGATCTATTCCTGGAGGTTCGTGCTACTCCTGGCCATACGTCAGGATGTGTCACCTATGTAACAGGGGAGGAACCTGATCAGCCTCATCCTAGGATGGCATTCACTGGGGATGCTCTGTTGATACGTGGATGTGGCAGGACAGATTTTCAGGGTGGAAGTTCAAAGCAGCTCTATGAATCTGTACACTCGCAGATCTTCACCCTGCCAAAGGACACATTGATCTATCCTGCTCACGATTACAAGGGATTCAATGTTAGTACTGTAGGCGAAGAGATGGTACACAATCCCCGGCTCACCAAGGATCTGGAAGAATTCAAGAAAATAATGGAAAATTTGAACCTGGCGTATCCAAAGATGATTGACATTGCAGTTCCAGCAAATTTGGTTTGTGGTTTGCAAGATCCAGCTTCTTAA

Coding sequence (CDS)

ATGCAAAGGTTTCGATTCTTACGAACTCCTCTGTTTCCTCTCAATCCTCCCTTCTTCGTCTCCCAAACTTCTCATCTTCTTCCGCTGAAACCCATTTCCAAAATTCCCACCAAGAGTATGGCGTCCTCATCATCTTCTTCCTCTTCTTCTAACCTCTTGTTCCGTCAACTCTTCGAGAAGGAATCCTCCACTTTCACCTACCTCCTCGCTGATGTTTCCCATCCGGACAAACCCGCGCTTTTGATTGACCCGGTTGACAAGACTGTAGATAGGGATCTAAACCTTGTTCGAGAACTGGGGCTGAAGCTTGTTTATGCTATGAACACTCATGTGCATGCTGATCATGTCACTGGCACTGGACTTATCAAGAGTAGAGTTCCAGGAGTGGAATCTGTAATCTCGAGGGCAAGTGGTTCAAAAGCTGATGTGTTGATTCAGCCTGGCGATAGAATTCCTTTTGGTGATCTATTCCTGGAGGTTCGTGCTACTCCTGGCCATACGTCAGGATGTGTCACCTATGTAACAGGGGAGGAACCTGATCAGCCTCATCCTAGGATGGCATTCACTGGGGATGCTCTGTTGATACGTGGATGTGGCAGGACAGATTTTCAGGGTGGAAGTTCAAAGCAGCTCTATGAATCTGTACACTCGCAGATCTTCACCCTGCCAAAGGACACATTGATCTATCCTGCTCACGATTACAAGGGATTCAATGTTAGTACTGTAGGCGAAGAGATGGTACACAATCCCCGGCTCACCAAGGATCTGGAAGAATTCAAGAAAATAATGGAAAATTTGAACCTGGCGTATCCAAAGATGATTGACATTGCAGTTCCAGCAAATTTGGTTTGTGGTTTGCAAGATCCAGCTTCTTAA

Protein sequence

MQRFRFLRTPLFPLNPPFFVSQTSHLLPLKPISKIPTKSMASSSSSSSSSNLLFRQLFEKESSTFTYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHVTGTGLIKSRVPGVESVISRASGSKADVLIQPGDRIPFGDLFLEVRATPGHTSGCVTYVTGEEPDQPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKGFNVSTVGEEMVHNPRLTKDLEEFKKIMENLNLAYPKMIDIAVPANLVCGLQDPAS
Homology
BLAST of MC11g_new0166 vs. ExPASy Swiss-Prot
Match: Q9C8L4 (Persulfide dioxygenase ETHE1 homolog, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=GLY3 PE=1 SV=3)

HSP 1 Score: 434.1 bits (1115), Expect = 1.2e-120
Identity = 220/291 (75.60%), Postives = 245/291 (84.19%), Query Frame = 0

Query: 1   MQRFRFLRTPLFPLNPPFFVSQTSHLLPLKPISKIPTKSMASSSSSSSSSNLLFRQLFEK 60
           M  F  LR  L  L  P F+S     L   P + + +   +SSS SSSSS LLFRQLFE 
Sbjct: 3   MTHFSRLRQLL--LLQPKFLSSQPRPLRSPPPTFLRSVMGSSSSFSSSSSKLLFRQLFEN 62

Query: 61  ESSTFTYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHVTGTG 120
           ESSTFTYLLADVSHPDKPALLIDPVDKTVDRDL L+ ELGLKL+YAMNTHVHADHVTGTG
Sbjct: 63  ESSTFTYLLADVSHPDKPALLIDPVDKTVDRDLKLIDELGLKLIYAMNTHVHADHVTGTG 122

Query: 121 LIKSRVPGVESVISRASGSKADVLIQPGDRIPFGDLFLEVRATPGHTSGCVTYVTGEEPD 180
           L+K+++PGV+SVIS+ASGSKAD+ ++PGD++  GD++LEVRATPGHT+GCVTYVTGE  D
Sbjct: 123 LLKTKLPGVKSVISKASGSKADLFLEPGDKVSIGDIYLEVRATPGHTAGCVTYVTGEGAD 182

Query: 181 QPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKGFNVS 240
           QP PRMAFTGDA+LIRGCGRTDFQ GSS QLYESVHSQIFTLPKDTLIYPAHDYKGF VS
Sbjct: 183 QPQPRMAFTGDAVLIRGCGRTDFQEGSSDQLYESVHSQIFTLPKDTLIYPAHDYKGFEVS 242

Query: 241 TVGEEMVHNPRLTKDLEEFKKIMENLNLAYPKMIDIAVPANLVCGLQDPAS 292
           TVGEEM HNPRLTKD E FK IM NLNL+YPKMID+AVPAN+VCGLQD  S
Sbjct: 243 TVGEEMQHNPRLTKDKETFKTIMSNLNLSYPKMIDVAVPANMVCGLQDVPS 291

BLAST of MC11g_new0166 vs. ExPASy Swiss-Prot
Match: Q9DCM0 (Persulfide dioxygenase ETHE1, mitochondrial OS=Mus musculus OX=10090 GN=Ethe1 PE=1 SV=2)

HSP 1 Score: 293.5 bits (750), Expect = 2.6e-78
Identity = 149/249 (59.84%), Postives = 183/249 (73.49%), Query Frame = 0

Query: 43  SSSSSSSSNLLFRQLFEKESSTFTYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLK 102
           S  S+S + +L RQ+FE +S T+TYLL D     + A+LIDPV +T  RD  L++ELGLK
Sbjct: 14  SQQSASGAPVLLRQMFEPKSCTYTYLLGD--RESREAVLIDPVLETAHRDAQLIKELGLK 73

Query: 103 LVYAMNTHVHADHVTGTGLIKSRVPGVESVISRASGSKADVLIQPGDRIPFGDLFLEVRA 162
           L+YA+NTH HADH+TGTG+++S +PG +SVISR SG++AD+ I  GD I FG   LE RA
Sbjct: 74  LLYAVNTHCHADHITGTGVLRSLLPGCQSVISRLSGAQADLHIGEGDSIRFGRFALETRA 133

Query: 163 TPGHTSGCVTYVTGEEPDQPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTL 222
           +PGHT GCVT+V  ++       MAFTGDALLIRGCGRTDFQ G +K LY SVH +IFTL
Sbjct: 134 SPGHTPGCVTFVLNDQ------SMAFTGDALLIRGCGRTDFQQGCAKTLYHSVHEKIFTL 193

Query: 223 PKDTLIYPAHDYKGFNVSTVGEEMVHNPRLTKDLEEFKKIMENLNLAYPKMIDIAVPANL 282
           P + LIYPAHDY G  VSTV EE   NPRLT   EEF K+M+NLNL  P+ IDIAVPAN+
Sbjct: 194 PGNCLIYPAHDYHGLTVSTVEEERTLNPRLTLSCEEFIKVMDNLNLPKPQQIDIAVPANM 253

Query: 283 VCGLQDPAS 292
            CG+Q P S
Sbjct: 254 RCGVQTPPS 254

BLAST of MC11g_new0166 vs. ExPASy Swiss-Prot
Match: O95571 (Persulfide dioxygenase ETHE1, mitochondrial OS=Homo sapiens OX=9606 GN=ETHE1 PE=1 SV=2)

HSP 1 Score: 292.0 bits (746), Expect = 7.6e-78
Identity = 149/249 (59.84%), Postives = 179/249 (71.89%), Query Frame = 0

Query: 43  SSSSSSSSNLLFRQLFEKESSTFTYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLK 102
           S    S + +L RQ+FE  S TFTYLL D     + A+LIDPV +T  RD  L++ELGL+
Sbjct: 14  SQRGGSGAPILLRQMFEPVSCTFTYLLGD--RESREAVLIDPVLETAPRDAQLIKELGLR 73

Query: 103 LVYAMNTHVHADHVTGTGLIKSRVPGVESVISRASGSKADVLIQPGDRIPFGDLFLEVRA 162
           L+YA+NTH HADH+TG+GL++S +PG +SVISR SG++AD+ I+ GD I FG   LE RA
Sbjct: 74  LLYAVNTHCHADHITGSGLLRSLLPGCQSVISRLSGAQADLHIEDGDSIRFGRFALETRA 133

Query: 163 TPGHTSGCVTYVTGEEPDQPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTL 222
           +PGHT GCVT+V  +        MAFTGDALLIRGCGRTDFQ G +K LY SVH +IFTL
Sbjct: 134 SPGHTPGCVTFVLNDH------SMAFTGDALLIRGCGRTDFQQGCAKTLYHSVHEKIFTL 193

Query: 223 PKDTLIYPAHDYKGFNVSTVGEEMVHNPRLTKDLEEFKKIMENLNLAYPKMIDIAVPANL 282
           P D LIYPAHDY GF VSTV EE   NPRLT   EEF KIM NLNL  P+ ID AVPAN+
Sbjct: 194 PGDCLIYPAHDYHGFTVSTVEEERTLNPRLTLSCEEFVKIMGNLNLPKPQQIDFAVPANM 253

Query: 283 VCGLQDPAS 292
            CG+Q P +
Sbjct: 254 RCGVQTPTA 254

BLAST of MC11g_new0166 vs. ExPASy Swiss-Prot
Match: Q3T094 (Persulfide dioxygenase ETHE1, mitochondrial OS=Bos taurus OX=9913 GN=ETHE1 PE=2 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 5.5e-76
Identity = 145/249 (58.23%), Postives = 179/249 (71.89%), Query Frame = 0

Query: 43  SSSSSSSSNLLFRQLFEKESSTFTYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLK 102
           S  + S + +L RQ+FE +S T+TYLL D     + A+LIDPV +T  RD  LV+ELGL+
Sbjct: 14  SQHTGSGAPVLLRQMFEPKSCTYTYLLGD--RESREAVLIDPVLETAQRDAQLVKELGLR 73

Query: 103 LVYAMNTHVHADHVTGTGLIKSRVPGVESVISRASGSKADVLIQPGDRIPFGDLFLEVRA 162
           L+YA+NTH HADH+TG+GL++S +PG +SVISR SG++AD  I+ GD I FG   LE RA
Sbjct: 74  LLYAVNTHCHADHITGSGLLRSLLPGCQSVISRLSGAQADWHIEDGDSIQFGRFALETRA 133

Query: 163 TPGHTSGCVTYVTGEEPDQPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTL 222
           +PGHT GCVT+V  +        MAFTGDALLIRGCGRTDFQ G ++ LY SVH +IFTL
Sbjct: 134 SPGHTPGCVTFVLNDH------SMAFTGDALLIRGCGRTDFQQGCAETLYHSVHEKIFTL 193

Query: 223 PKDTLIYPAHDYKGFNVSTVGEEMVHNPRLTKDLEEFKKIMENLNLAYPKMIDIAVPANL 282
           P + LIYPAHDY G  VSTV EE   NPRLT   EEF K+M+ LNL  P+ ID AVPAN+
Sbjct: 194 PGNCLIYPAHDYHGLTVSTVEEERTLNPRLTLSCEEFVKVMDKLNLPKPQQIDFAVPANM 253

Query: 283 VCGLQDPAS 292
            CG+Q P S
Sbjct: 254 RCGIQTPPS 254

BLAST of MC11g_new0166 vs. ExPASy Swiss-Prot
Match: Q8UAA9 (Beta-lactamase hydrolase-like protein OS=Agrobacterium fabrum (strain C58 / ATCC 33970) OX=176299 GN=blh PE=2 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 6.6e-21
Identity = 75/221 (33.94%), Postives = 105/221 (47.51%), Query Frame = 0

Query: 90  DRDLNLVRELGLKLVYAMNTHVHADHVTGTGLIK----------SRVPGVESVIS----- 149
           D  L+ V+  GL + + ++TH HADH +    +K          ++V GV+ +       
Sbjct: 192 DAILDYVKRHGLSVEWILDTHPHADHFSAADYLKQKTGAKTAIGAKVTGVQKLWQEKYNW 251

Query: 150 ---RASGSKADVLIQPGDRIPFGDLFLEVRATPGHTSGCVTYVTGEEPDQPHPRMAFTGD 209
              +  GS+ D L + GDR   G L   V  +PGHT   VTYV G          AF  D
Sbjct: 252 SDFKTDGSQWDQLFEAGDRFSIGSLEARVLFSPGHTLASVTYVVG--------NAAFVHD 311

Query: 210 ALLI--RGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKGFN-----VSTVGE 269
            L +   G  R DF GGS+KQL+ S+   I  LP DT ++  HDY+         STVGE
Sbjct: 312 TLFMPDSGTARADFPGGSAKQLWASI-QDILALPDDTRLFTGHDYQPGGRAPKWESTVGE 371

Query: 270 EMVHNPRLTKDLEE-FKKIME--NLNLAYPKMIDIAVPANL 283
           +   NP L    EE F ++ E  +  L  PK+I  A+  N+
Sbjct: 372 QTRSNPHLAGMTEEDFVRLREARDRTLPMPKLILHALQVNI 403

BLAST of MC11g_new0166 vs. NCBI nr
Match: XP_022142030.1 (persulfide dioxygenase ETHE1 homolog, mitochondrial [Momordica charantia])

HSP 1 Score: 580 bits (1494), Expect = 1.29e-208
Identity = 291/291 (100.00%), Postives = 291/291 (100.00%), Query Frame = 0

Query: 1   MQRFRFLRTPLFPLNPPFFVSQTSHLLPLKPISKIPTKSMASSSSSSSSSNLLFRQLFEK 60
           MQRFRFLRTPLFPLNPPFFVSQTSHLLPLKPISKIPTKSMASSSSSSSSSNLLFRQLFEK
Sbjct: 1   MQRFRFLRTPLFPLNPPFFVSQTSHLLPLKPISKIPTKSMASSSSSSSSSNLLFRQLFEK 60

Query: 61  ESSTFTYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHVTGTG 120
           ESSTFTYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHVTGTG
Sbjct: 61  ESSTFTYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHVTGTG 120

Query: 121 LIKSRVPGVESVISRASGSKADVLIQPGDRIPFGDLFLEVRATPGHTSGCVTYVTGEEPD 180
           LIKSRVPGVESVISRASGSKADVLIQPGDRIPFGDLFLEVRATPGHTSGCVTYVTGEEPD
Sbjct: 121 LIKSRVPGVESVISRASGSKADVLIQPGDRIPFGDLFLEVRATPGHTSGCVTYVTGEEPD 180

Query: 181 QPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKGFNVS 240
           QPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKGFNVS
Sbjct: 181 QPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKGFNVS 240

Query: 241 TVGEEMVHNPRLTKDLEEFKKIMENLNLAYPKMIDIAVPANLVCGLQDPAS 291
           TVGEEMVHNPRLTKDLEEFKKIMENLNLAYPKMIDIAVPANLVCGLQDPAS
Sbjct: 241 TVGEEMVHNPRLTKDLEEFKKIMENLNLAYPKMIDIAVPANLVCGLQDPAS 291

BLAST of MC11g_new0166 vs. NCBI nr
Match: XP_022976826.1 (persulfide dioxygenase ETHE1 homolog, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 514 bits (1325), Expect = 8.59e-183
Identity = 262/295 (88.81%), Postives = 275/295 (93.22%), Query Frame = 0

Query: 1   MQRFRFLRTPLF-PLNPPFFVSQTSHLLPLKPISKIPTKSMAS---SSSSSSSSNLLFRQ 60
           MQ FRFLR PLF P N  FFVSQ  HLLP+K IS+I TK+ +S   SSSSSSSS LLFRQ
Sbjct: 1   MQSFRFLRIPLFSPPNALFFVSQPPHLLPVKSISRISTKNFSSQMESSSSSSSSKLLFRQ 60

Query: 61  LFEKESSTFTYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHV 120
           LFEKESST+TYLLADVSHPDKPALLIDPVD+TVDRDLNLVRELGLKLVYAMNTHVHADHV
Sbjct: 61  LFEKESSTYTYLLADVSHPDKPALLIDPVDRTVDRDLNLVRELGLKLVYAMNTHVHADHV 120

Query: 121 TGTGLIKSRVPGVESVISRASGSKADVLIQPGDRIPFGDLFLEVRATPGHTSGCVTYVTG 180
           TGTGLIKS+ PG +SVISRASGSKADVLI PGDRIPFGDLFLEVRATPGHTSGCVTYVTG
Sbjct: 121 TGTGLIKSKFPGAKSVISRASGSKADVLIGPGDRIPFGDLFLEVRATPGHTSGCVTYVTG 180

Query: 181 EEPDQPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKG 240
           +EPDQPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKG
Sbjct: 181 DEPDQPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKG 240

Query: 241 FNVSTVGEEMVHNPRLTKDLEEFKKIMENLNLAYPKMIDIAVPANLVCGLQDPAS 291
           FNVSTVGEE+V+NPRLTKDLEEFKKIMENLNL YPKM+D+AVPANLVCGLQDP S
Sbjct: 241 FNVSTVGEEIVYNPRLTKDLEEFKKIMENLNLPYPKMMDLAVPANLVCGLQDPTS 295

BLAST of MC11g_new0166 vs. NCBI nr
Match: XP_038900177.1 (persulfide dioxygenase ETHE1 homolog, mitochondrial [Benincasa hispida])

HSP 1 Score: 514 bits (1324), Expect = 1.18e-182
Identity = 260/293 (88.74%), Postives = 272/293 (92.83%), Query Frame = 0

Query: 1   MQRFRFLRTPLFPLNPPFFVSQTSHLLPLKPISKIPTKSMAS---SSSSSSSSNLLFRQL 60
           MQ FRFLR PLFP N  FFVS T HLLP+KPIS+IP K  +S   SS SSSSS LLFRQL
Sbjct: 1   MQGFRFLRIPLFPSNALFFVSPTPHLLPVKPISRIPIKYFSSQMGSSPSSSSSKLLFRQL 60

Query: 61  FEKESSTFTYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHVT 120
           FEKESST+TYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHVT
Sbjct: 61  FEKESSTYTYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHVT 120

Query: 121 GTGLIKSRVPGVESVISRASGSKADVLIQPGDRIPFGDLFLEVRATPGHTSGCVTYVTGE 180
           GTGLIKS+ PG +SVISRASGSKADVLI+PGDRI FGDLFLEVRATPGHTSGCVTYVTG+
Sbjct: 121 GTGLIKSKFPGAKSVISRASGSKADVLIEPGDRISFGDLFLEVRATPGHTSGCVTYVTGD 180

Query: 181 EPDQPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKGF 240
           EPDQPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKGF
Sbjct: 181 EPDQPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKGF 240

Query: 241 NVSTVGEEMVHNPRLTKDLEEFKKIMENLNLAYPKMIDIAVPANLVCGLQDPA 290
           + STVGEEM +NPRLTKDLEEFKKIME+LNL YPKM+DIAVPANLVCGLQDPA
Sbjct: 241 SASTVGEEMKYNPRLTKDLEEFKKIMEHLNLPYPKMMDIAVPANLVCGLQDPA 293

BLAST of MC11g_new0166 vs. NCBI nr
Match: KAG6591786.1 (Persulfide dioxygenase ETHE1-like, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 513 bits (1321), Expect = 3.50e-182
Identity = 261/295 (88.47%), Postives = 274/295 (92.88%), Query Frame = 0

Query: 1   MQRFRFLRTPLF-PLNPPFFVSQTSHLLPLKPISKIPTKSMASS---SSSSSSSNLLFRQ 60
           MQ FRFLR PLF P N  FFVSQ  HLLP+K IS+I TK+ +S    S SSSSS LLFRQ
Sbjct: 1   MQSFRFLRIPLFSPPNALFFVSQPPHLLPVKSISRISTKNFSSQMELSLSSSSSKLLFRQ 60

Query: 61  LFEKESSTFTYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHV 120
           LFEK+SST+TYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHV
Sbjct: 61  LFEKDSSTYTYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHV 120

Query: 121 TGTGLIKSRVPGVESVISRASGSKADVLIQPGDRIPFGDLFLEVRATPGHTSGCVTYVTG 180
           TGTGLIKS+ PG +SVISRASGSKADVLI+PGDRIPFGDLFLEVRATPGHTSGCVTYVTG
Sbjct: 121 TGTGLIKSKFPGAKSVISRASGSKADVLIEPGDRIPFGDLFLEVRATPGHTSGCVTYVTG 180

Query: 181 EEPDQPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKG 240
           +EPDQPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKG
Sbjct: 181 DEPDQPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKG 240

Query: 241 FNVSTVGEEMVHNPRLTKDLEEFKKIMENLNLAYPKMIDIAVPANLVCGLQDPAS 291
           FNVSTVGEE+V+NPRLTKDLEEFKKIMENLNL YPKM+DIAVPANLVCGLQDP S
Sbjct: 241 FNVSTVGEEIVYNPRLTKDLEEFKKIMENLNLPYPKMMDIAVPANLVCGLQDPTS 295

BLAST of MC11g_new0166 vs. NCBI nr
Match: XP_023536198.1 (persulfide dioxygenase ETHE1 homolog, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 511 bits (1317), Expect = 1.42e-181
Identity = 261/295 (88.47%), Postives = 274/295 (92.88%), Query Frame = 0

Query: 1   MQRFRFLRTPLF-PLNPPFFVSQTSHLLPLKPISKIPTKSMAS---SSSSSSSSNLLFRQ 60
           MQ FRFLR P F P N  FFVSQ  HLLP+K IS+I TK+ +S   SS SSSSS LLFRQ
Sbjct: 1   MQSFRFLRIPSFSPPNALFFVSQPPHLLPVKSISRISTKNFSSQMESSLSSSSSKLLFRQ 60

Query: 61  LFEKESSTFTYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHV 120
           LFEKESST+TYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHV
Sbjct: 61  LFEKESSTYTYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHV 120

Query: 121 TGTGLIKSRVPGVESVISRASGSKADVLIQPGDRIPFGDLFLEVRATPGHTSGCVTYVTG 180
           TGTGLIKS+ PG +SVISRASGSKADVLI+PGDRIPFGDLFLEVRATPGHTSGCVTYVTG
Sbjct: 121 TGTGLIKSKFPGAKSVISRASGSKADVLIEPGDRIPFGDLFLEVRATPGHTSGCVTYVTG 180

Query: 181 EEPDQPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKG 240
           +EPDQPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKG
Sbjct: 181 DEPDQPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKG 240

Query: 241 FNVSTVGEEMVHNPRLTKDLEEFKKIMENLNLAYPKMIDIAVPANLVCGLQDPAS 291
           FNVSTVGEE+V+NPRLTK+LEEFKKIMENLNL YPKM+DIAVPANLVCGLQDP S
Sbjct: 241 FNVSTVGEEIVYNPRLTKNLEEFKKIMENLNLPYPKMMDIAVPANLVCGLQDPTS 295

BLAST of MC11g_new0166 vs. ExPASy TrEMBL
Match: A0A6J1CJS0 (persulfide dioxygenase ETHE1 homolog, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111012255 PE=4 SV=1)

HSP 1 Score: 580 bits (1494), Expect = 6.26e-209
Identity = 291/291 (100.00%), Postives = 291/291 (100.00%), Query Frame = 0

Query: 1   MQRFRFLRTPLFPLNPPFFVSQTSHLLPLKPISKIPTKSMASSSSSSSSSNLLFRQLFEK 60
           MQRFRFLRTPLFPLNPPFFVSQTSHLLPLKPISKIPTKSMASSSSSSSSSNLLFRQLFEK
Sbjct: 1   MQRFRFLRTPLFPLNPPFFVSQTSHLLPLKPISKIPTKSMASSSSSSSSSNLLFRQLFEK 60

Query: 61  ESSTFTYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHVTGTG 120
           ESSTFTYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHVTGTG
Sbjct: 61  ESSTFTYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHVTGTG 120

Query: 121 LIKSRVPGVESVISRASGSKADVLIQPGDRIPFGDLFLEVRATPGHTSGCVTYVTGEEPD 180
           LIKSRVPGVESVISRASGSKADVLIQPGDRIPFGDLFLEVRATPGHTSGCVTYVTGEEPD
Sbjct: 121 LIKSRVPGVESVISRASGSKADVLIQPGDRIPFGDLFLEVRATPGHTSGCVTYVTGEEPD 180

Query: 181 QPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKGFNVS 240
           QPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKGFNVS
Sbjct: 181 QPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKGFNVS 240

Query: 241 TVGEEMVHNPRLTKDLEEFKKIMENLNLAYPKMIDIAVPANLVCGLQDPAS 291
           TVGEEMVHNPRLTKDLEEFKKIMENLNLAYPKMIDIAVPANLVCGLQDPAS
Sbjct: 241 TVGEEMVHNPRLTKDLEEFKKIMENLNLAYPKMIDIAVPANLVCGLQDPAS 291

BLAST of MC11g_new0166 vs. ExPASy TrEMBL
Match: A0A6J1IHY8 (persulfide dioxygenase ETHE1 homolog, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111477087 PE=4 SV=1)

HSP 1 Score: 514 bits (1325), Expect = 4.16e-183
Identity = 262/295 (88.81%), Postives = 275/295 (93.22%), Query Frame = 0

Query: 1   MQRFRFLRTPLF-PLNPPFFVSQTSHLLPLKPISKIPTKSMAS---SSSSSSSSNLLFRQ 60
           MQ FRFLR PLF P N  FFVSQ  HLLP+K IS+I TK+ +S   SSSSSSSS LLFRQ
Sbjct: 1   MQSFRFLRIPLFSPPNALFFVSQPPHLLPVKSISRISTKNFSSQMESSSSSSSSKLLFRQ 60

Query: 61  LFEKESSTFTYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHV 120
           LFEKESST+TYLLADVSHPDKPALLIDPVD+TVDRDLNLVRELGLKLVYAMNTHVHADHV
Sbjct: 61  LFEKESSTYTYLLADVSHPDKPALLIDPVDRTVDRDLNLVRELGLKLVYAMNTHVHADHV 120

Query: 121 TGTGLIKSRVPGVESVISRASGSKADVLIQPGDRIPFGDLFLEVRATPGHTSGCVTYVTG 180
           TGTGLIKS+ PG +SVISRASGSKADVLI PGDRIPFGDLFLEVRATPGHTSGCVTYVTG
Sbjct: 121 TGTGLIKSKFPGAKSVISRASGSKADVLIGPGDRIPFGDLFLEVRATPGHTSGCVTYVTG 180

Query: 181 EEPDQPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKG 240
           +EPDQPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKG
Sbjct: 181 DEPDQPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKG 240

Query: 241 FNVSTVGEEMVHNPRLTKDLEEFKKIMENLNLAYPKMIDIAVPANLVCGLQDPAS 291
           FNVSTVGEE+V+NPRLTKDLEEFKKIMENLNL YPKM+D+AVPANLVCGLQDP S
Sbjct: 241 FNVSTVGEEIVYNPRLTKDLEEFKKIMENLNLPYPKMMDLAVPANLVCGLQDPTS 295

BLAST of MC11g_new0166 vs. ExPASy TrEMBL
Match: A0A6J1FEX7 (persulfide dioxygenase ETHE1 homolog, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111443326 PE=4 SV=1)

HSP 1 Score: 509 bits (1311), Expect = 5.65e-181
Identity = 259/295 (87.80%), Postives = 272/295 (92.20%), Query Frame = 0

Query: 1   MQRFRFLRTPLF-PLNPPFFVSQTSHLLPLKPISKIPTKSMASS---SSSSSSSNLLFRQ 60
           MQ  RFLR PLF P N  FFVSQ  HLLP+K IS+I TK+ +S    S SSSSS LLFRQ
Sbjct: 1   MQSLRFLRIPLFSPPNALFFVSQPPHLLPVKSISRISTKNFSSQMELSLSSSSSKLLFRQ 60

Query: 61  LFEKESSTFTYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHV 120
           LFEK+SST+TYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHV
Sbjct: 61  LFEKDSSTYTYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHV 120

Query: 121 TGTGLIKSRVPGVESVISRASGSKADVLIQPGDRIPFGDLFLEVRATPGHTSGCVTYVTG 180
           TGTGLIKS+ PG +SVISRASGSKADV I+PGDRIPFGDLFLEVRATPGHTSGCVTYVTG
Sbjct: 121 TGTGLIKSKFPGAKSVISRASGSKADVFIEPGDRIPFGDLFLEVRATPGHTSGCVTYVTG 180

Query: 181 EEPDQPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKG 240
           +EPDQPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKG
Sbjct: 181 DEPDQPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKG 240

Query: 241 FNVSTVGEEMVHNPRLTKDLEEFKKIMENLNLAYPKMIDIAVPANLVCGLQDPAS 291
           FNVSTVGEE+V+NPRLTKDLEEFKKIMENLNL YPKM+DIAVPANLVCGLQDP S
Sbjct: 241 FNVSTVGEEIVYNPRLTKDLEEFKKIMENLNLPYPKMMDIAVPANLVCGLQDPTS 295

BLAST of MC11g_new0166 vs. ExPASy TrEMBL
Match: A0A1S3CJS9 (persulfide dioxygenase ETHE1 homolog, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103501765 PE=4 SV=1)

HSP 1 Score: 501 bits (1291), Expect = 6.07e-178
Identity = 254/293 (86.69%), Postives = 269/293 (91.81%), Query Frame = 0

Query: 1   MQRFRFLRTPLFPLNPPFFVSQTSHLLPLKPISKIPTKSMAS---SSSSSSSSNLLFRQL 60
           M   RFLR PLF  N   F SQT HLL +KPIS IP ++ +S   SS SSSSS LLFRQL
Sbjct: 1   MHSCRFLRVPLFQPNALCFFSQTPHLLSVKPISSIPVRNFSSQMGSSPSSSSSKLLFRQL 60

Query: 61  FEKESSTFTYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHVT 120
           FEK+SST+TYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHVT
Sbjct: 61  FEKDSSTYTYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHVT 120

Query: 121 GTGLIKSRVPGVESVISRASGSKADVLIQPGDRIPFGDLFLEVRATPGHTSGCVTYVTGE 180
           GTGLIK++ PG +SVISRASGSKADVLI+PGDRI FGDLFLEVRATPGHTSGCVTYVTG+
Sbjct: 121 GTGLIKNKFPGAKSVISRASGSKADVLIEPGDRISFGDLFLEVRATPGHTSGCVTYVTGD 180

Query: 181 EPDQPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKGF 240
           EPDQP+PRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKGF
Sbjct: 181 EPDQPNPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKGF 240

Query: 241 NVSTVGEEMVHNPRLTKDLEEFKKIMENLNLAYPKMIDIAVPANLVCGLQDPA 290
           +VSTVGEEM +NPRLTKDLEEFKKIMENLNLAYPKM+DIAVPANLVCGLQDPA
Sbjct: 241 SVSTVGEEMTYNPRLTKDLEEFKKIMENLNLAYPKMMDIAVPANLVCGLQDPA 293

BLAST of MC11g_new0166 vs. ExPASy TrEMBL
Match: A0A6J1FJM1 (persulfide dioxygenase ETHE1 homolog, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111446332 PE=4 SV=1)

HSP 1 Score: 489 bits (1259), Expect = 3.94e-173
Identity = 248/294 (84.35%), Postives = 269/294 (91.50%), Query Frame = 0

Query: 1   MQRFRFLRTPLFPLNPPFFVSQTSHLLPLKPISKIPTKSMAS---SSSSSSSSNLLFRQL 60
           MQ  RFLR P FP N  FF     HLLPLKPISKI  K+ +S   SS SSSSS LLFRQL
Sbjct: 1   MQSIRFLRIPRFPPNVLFF-----HLLPLKPISKISIKNFSSQIGSSPSSSSSKLLFRQL 60

Query: 61  FEKESSTFTYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHVT 120
           FEKESST+TYLLA+VSHPDKPALLIDPVDKTVDRDLNL+RELGLKLVYAMNTHVHADH+T
Sbjct: 61  FEKESSTYTYLLANVSHPDKPALLIDPVDKTVDRDLNLIRELGLKLVYAMNTHVHADHIT 120

Query: 121 GTGLIKSRVPGVESVISRASGSKADVLIQPGDRIPFGDLFLEVRATPGHTSGCVTYVTGE 180
           GTGLIK++VPG +SVISRASGSKAD+LI+PGDRI FGDLFLEVRATPGHT+GCVTYVTG+
Sbjct: 121 GTGLIKTKVPGAKSVISRASGSKADLLIEPGDRISFGDLFLEVRATPGHTAGCVTYVTGD 180

Query: 181 EPDQPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKGF 240
           EPDQPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDY GF
Sbjct: 181 EPDQPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYAGF 240

Query: 241 NVSTVGEEMVHNPRLTKDLEEFKKIMENLNLAYPKMIDIAVPANLVCGLQDPAS 291
           +VS+VGEE+ +NPRL+KDLEEFKKIMENLNLAYPK++D+AVPANLVCGLQ PAS
Sbjct: 241 SVSSVGEEIENNPRLSKDLEEFKKIMENLNLAYPKVMDMAVPANLVCGLQHPAS 289

BLAST of MC11g_new0166 vs. TAIR 10
Match: AT1G53580.1 (glyoxalase II 3 )

HSP 1 Score: 434.1 bits (1115), Expect = 8.8e-122
Identity = 220/291 (75.60%), Postives = 245/291 (84.19%), Query Frame = 0

Query: 1   MQRFRFLRTPLFPLNPPFFVSQTSHLLPLKPISKIPTKSMASSSSSSSSSNLLFRQLFEK 60
           M  F  LR  L  L  P F+S     L   P + + +   +SSS SSSSS LLFRQLFE 
Sbjct: 3   MTHFSRLRQLL--LLQPKFLSSQPRPLRSPPPTFLRSVMGSSSSFSSSSSKLLFRQLFEN 62

Query: 61  ESSTFTYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHVTGTG 120
           ESSTFTYLLADVSHPDKPALLIDPVDKTVDRDL L+ ELGLKL+YAMNTHVHADHVTGTG
Sbjct: 63  ESSTFTYLLADVSHPDKPALLIDPVDKTVDRDLKLIDELGLKLIYAMNTHVHADHVTGTG 122

Query: 121 LIKSRVPGVESVISRASGSKADVLIQPGDRIPFGDLFLEVRATPGHTSGCVTYVTGEEPD 180
           L+K+++PGV+SVIS+ASGSKAD+ ++PGD++  GD++LEVRATPGHT+GCVTYVTGE  D
Sbjct: 123 LLKTKLPGVKSVISKASGSKADLFLEPGDKVSIGDIYLEVRATPGHTAGCVTYVTGEGAD 182

Query: 181 QPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKGFNVS 240
           QP PRMAFTGDA+LIRGCGRTDFQ GSS QLYESVHSQIFTLPKDTLIYPAHDYKGF VS
Sbjct: 183 QPQPRMAFTGDAVLIRGCGRTDFQEGSSDQLYESVHSQIFTLPKDTLIYPAHDYKGFEVS 242

Query: 241 TVGEEMVHNPRLTKDLEEFKKIMENLNLAYPKMIDIAVPANLVCGLQDPAS 292
           TVGEEM HNPRLTKD E FK IM NLNL+YPKMID+AVPAN+VCGLQD  S
Sbjct: 243 TVGEEMQHNPRLTKDKETFKTIMSNLNLSYPKMIDVAVPANMVCGLQDVPS 291

BLAST of MC11g_new0166 vs. TAIR 10
Match: AT1G53580.2 (glyoxalase II 3 )

HSP 1 Score: 431.8 bits (1109), Expect = 4.4e-121
Identity = 219/291 (75.26%), Postives = 244/291 (83.85%), Query Frame = 0

Query: 1   MQRFRFLRTPLFPLNPPFFVSQTSHLLPLKPISKIPTKSMASSSSSSSSSNLLFRQLFEK 60
           M  F  LR  L  L  P F+S     L   P + + +   +SSS SSSSS LLFRQLFE 
Sbjct: 3   MTHFSRLRQLL--LLQPKFLSSQPRPLRSPPPTFLRSVMGSSSSFSSSSSKLLFRQLFEN 62

Query: 61  ESSTFTYLLADVSHPDKPALLIDPVDKTVDRDLNLVRELGLKLVYAMNTHVHADHVTGTG 120
           ESSTFTYLLADVSHPDKPALLIDPVDKTVDRDL L+ ELGLKL+YAMNTHVHADHVTGTG
Sbjct: 63  ESSTFTYLLADVSHPDKPALLIDPVDKTVDRDLKLIDELGLKLIYAMNTHVHADHVTGTG 122

Query: 121 LIKSRVPGVESVISRASGSKADVLIQPGDRIPFGDLFLEVRATPGHTSGCVTYVTGEEPD 180
           L+K+++PGV+SVIS+ASGSKAD+ ++PGD++  GD++LEVRATPGHT+GCVTYVTGE  D
Sbjct: 123 LLKTKLPGVKSVISKASGSKADLFLEPGDKVSIGDIYLEVRATPGHTAGCVTYVTGEGAD 182

Query: 181 QPHPRMAFTGDALLIRGCGRTDFQGGSSKQLYESVHSQIFTLPKDTLIYPAHDYKGFNVS 240
           QP PRMAFTGDA+LIRGCGRTDFQ GSS QLYESVHSQIFTLPKDTLIYPAHDYKGF V 
Sbjct: 183 QPQPRMAFTGDAVLIRGCGRTDFQEGSSDQLYESVHSQIFTLPKDTLIYPAHDYKGFEVI 242

Query: 241 TVGEEMVHNPRLTKDLEEFKKIMENLNLAYPKMIDIAVPANLVCGLQDPAS 292
           TVGEEM HNPRLTKD E FK IM NLNL+YPKMID+AVPAN+VCGLQD  S
Sbjct: 243 TVGEEMQHNPRLTKDKETFKTIMSNLNLSYPKMIDVAVPANMVCGLQDVPS 291

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C8L41.2e-12075.60Persulfide dioxygenase ETHE1 homolog, mitochondrial OS=Arabidopsis thaliana OX=3... [more]
Q9DCM02.6e-7859.84Persulfide dioxygenase ETHE1, mitochondrial OS=Mus musculus OX=10090 GN=Ethe1 PE... [more]
O955717.6e-7859.84Persulfide dioxygenase ETHE1, mitochondrial OS=Homo sapiens OX=9606 GN=ETHE1 PE=... [more]
Q3T0945.5e-7658.23Persulfide dioxygenase ETHE1, mitochondrial OS=Bos taurus OX=9913 GN=ETHE1 PE=2 ... [more]
Q8UAA96.6e-2133.94Beta-lactamase hydrolase-like protein OS=Agrobacterium fabrum (strain C58 / ATCC... [more]
Match NameE-valueIdentityDescription
XP_022142030.11.29e-208100.00persulfide dioxygenase ETHE1 homolog, mitochondrial [Momordica charantia][more]
XP_022976826.18.59e-18388.81persulfide dioxygenase ETHE1 homolog, mitochondrial-like [Cucurbita maxima][more]
XP_038900177.11.18e-18288.74persulfide dioxygenase ETHE1 homolog, mitochondrial [Benincasa hispida][more]
KAG6591786.13.50e-18288.47Persulfide dioxygenase ETHE1-like, mitochondrial, partial [Cucurbita argyrosperm... [more]
XP_023536198.11.42e-18188.47persulfide dioxygenase ETHE1 homolog, mitochondrial-like [Cucurbita pepo subsp. ... [more]
Match NameE-valueIdentityDescription
A0A6J1CJS06.26e-209100.00persulfide dioxygenase ETHE1 homolog, mitochondrial OS=Momordica charantia OX=36... [more]
A0A6J1IHY84.16e-18388.81persulfide dioxygenase ETHE1 homolog, mitochondrial-like OS=Cucurbita maxima OX=... [more]
A0A6J1FEX75.65e-18187.80persulfide dioxygenase ETHE1 homolog, mitochondrial OS=Cucurbita moschata OX=366... [more]
A0A1S3CJS96.07e-17886.69persulfide dioxygenase ETHE1 homolog, mitochondrial OS=Cucumis melo OX=3656 GN=L... [more]
A0A6J1FJM13.94e-17384.35persulfide dioxygenase ETHE1 homolog, mitochondrial-like OS=Cucurbita moschata O... [more]
Match NameE-valueIdentityDescription
AT1G53580.18.8e-12275.60glyoxalase II 3 [more]
AT1G53580.24.4e-12175.26glyoxalase II 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001279Metallo-beta-lactamaseSMARTSM00849Lactamase_B_5acoord: 63..232
e-value: 2.1E-22
score: 90.4
IPR001279Metallo-beta-lactamasePFAMPF00753Lactamase_Bcoord: 63..232
e-value: 7.8E-11
score: 42.3
IPR036866Ribonuclease Z/Hydroxyacylglutathione hydrolase-likeGENE3D3.60.15.10coord: 53..281
e-value: 2.8E-91
score: 306.5
IPR036866Ribonuclease Z/Hydroxyacylglutathione hydrolase-likeSUPERFAMILY56281Metallo-hydrolase/oxidoreductasecoord: 76..276
NoneNo IPR availablePANTHERPTHR43084PERSULFIDE DIOXYGENASE ETHE1coord: 22..288
NoneNo IPR availablePANTHERPTHR43084:SF10BNAC03G69760D PROTEINcoord: 22..288
IPR044528Persulfide dioxygenase-like, MBL-fold metallo-hydrolase domainCDDcd07724POD-like_MBL-foldcoord: 53..234
e-value: 1.15183E-78
score: 234.601

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC11g_new0166.1MC11g_new0166.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009793 embryo development ending in seed dormancy
biological_process GO:0009960 endosperm development
biological_process GO:0006749 glutathione metabolic process
biological_process GO:0070813 hydrogen sulfide metabolic process
cellular_component GO:0005739 mitochondrion
molecular_function GO:0016788 hydrolase activity, acting on ester bonds
molecular_function GO:0050313 sulfur dioxygenase activity