MC11g_new0086 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC11g_new0086
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	17.3 kDa class II heat shock protein-like
Location	MC11: 2757825 .. 2758307 (-)
RNA-Seq Expression	MC11g_new0086
Synteny	MC11g_new0086

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTTCAGGATCATGGGCATGGACTCCCCGATCTTCTCCACCCTCCAGCACGTGATGGATCTCGCCGACGACGGCGACAATAAGTCGTTCAACGCCCCGACGCGCACCTACGTCCGGGATGCCAAGGCGATGGCGGCGACGCCAGCCGACATCAAGGAGTATCCTAACTCCTACGTCTTCGTCGTCGACATGCCGGGGCTGAAGGTCGGCGACATCAAGGTCCAGGTTGAGGATGACAATGTGCTGCTCATAAGTGGGGAGAGGAAGAGGGAGGAGGACAAAGAGGGGGCCAATGCCGCCAAGTACGTGAGAATGGAAAGGAGGGTTGGTAAGTTTATGAGGAAATTTGTGCTGCCGGAAAATGCCAACACCGATGCCATCTCCGCCGTGTGCCAGGATGGTGTGCTCACTGTCACTGTGCAGAAGTTGCCACCACCTCAGCCCAAGAAGCCCAAGATCGTTGAAGTCAAGGTCAATTGA

mRNA sequence

ATGGATTTCAGGATCATGGGCATGGACTCCCCGATCTTCTCCACCCTCCAGCACGTGATGGATCTCGCCGACGACGGCGACAATAAGTCGTTCAACGCCCCGACGCGCACCTACGTCCGGGATGCCAAGGCGATGGCGGCGACGCCAGCCGACATCAAGGAGTATCCTAACTCCTACGTCTTCGTCGTCGACATGCCGGGGCTGAAGGTCGGCGACATCAAGGTCCAGGTTGAGGATGACAATGTGCTGCTCATAAGTGGGGAGAGGAAGAGGGAGGAGGACAAAGAGGGGGCCAATGCCGCCAAGTACGTGAGAATGGAAAGGAGGGTTGGTAAGTTTATGAGGAAATTTGTGCTGCCGGAAAATGCCAACACCGATGCCATCTCCGCCGTGTGCCAGGATGGTGTGCTCACTGTCACTGTGCAGAAGTTGCCACCACCTCAGCCCAAGAAGCCCAAGATCGTTGAAGTCAAGGTCAATTGA

Coding sequence (CDS)

ATGGATTTCAGGATCATGGGCATGGACTCCCCGATCTTCTCCACCCTCCAGCACGTGATGGATCTCGCCGACGACGGCGACAATAAGTCGTTCAACGCCCCGACGCGCACCTACGTCCGGGATGCCAAGGCGATGGCGGCGACGCCAGCCGACATCAAGGAGTATCCTAACTCCTACGTCTTCGTCGTCGACATGCCGGGGCTGAAGGTCGGCGACATCAAGGTCCAGGTTGAGGATGACAATGTGCTGCTCATAAGTGGGGAGAGGAAGAGGGAGGAGGACAAAGAGGGGGCCAATGCCGCCAAGTACGTGAGAATGGAAAGGAGGGTTGGTAAGTTTATGAGGAAATTTGTGCTGCCGGAAAATGCCAACACCGATGCCATCTCCGCCGTGTGCCAGGATGGTGTGCTCACTGTCACTGTGCAGAAGTTGCCACCACCTCAGCCCAAGAAGCCCAAGATCGTTGAAGTCAAGGTCAATTGA

Protein sequence

MDFRIMGMDSPIFSTLQHVMDLADDGDNKSFNAPTRTYVRDAKAMAATPADIKEYPNSYVFVVDMPGLKVGDIKVQVEDDNVLLISGERKREEDKEGANAAKYVRMERRVGKFMRKFVLPENANTDAISAVCQDGVLTVTVQKLPPPQPKKPKIVEVKVN

Homology

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BLAST of MC11g_new0086 vs. ExPASy Swiss-Prot
Match: P05477 (17.9 kDa class II heat shock protein OS=Glycine max OX=3847 GN=HSP17.9-D PE=3 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 1.1e-67
Identity = 127/161 (78.88%), Postives = 148/161 (91.93%), Query Frame = 0


Query: 1   MDFRIMGMDSPIFSTLQHVMDLADD--GDNKSFNAPTRTYVRDAKAMAATPADIKEYPNS 60
           MDFR+MG++SP+F TLQH+MD+++D  GDNK+ NAPT +YVRDAKAMAATPAD+KEYPNS
Sbjct: 1   MDFRVMGLESPLFHTLQHMMDMSEDGAGDNKTHNAPTWSYVRDAKAMAATPADVKEYPNS 60

Query: 61  YVFVVDMPGLKVGDIKVQVEDDNVLLISGERKREEDKEGANAAKYVRMERRVGKFMRKFV 120
           YVF +DMPGLK GDIKVQVEDDN+LLI GERKR+E+KEG   AKY+RMERRVGK MRKFV
Sbjct: 61  YVFEIDMPGLKSGDIKVQVEDDNLLLICGERKRDEEKEG---AKYLRMERRVGKLMRKFV 120

Query: 121 LPENANTDAISAVCQDGVLTVTVQKLPPPQPKKPKIVEVKV 160
           LPENANTDAISAVCQDGVL+VTVQKLPPP+PKKP+ ++VKV
Sbjct: 121 LPENANTDAISAVCQDGVLSVTVQKLPPPEPKKPRTIQVKV 158

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BLAST of MC11g_new0086 vs. ExPASy Swiss-Prot
Match: O82013 (17.3 kDa class II heat shock protein OS=Solanum peruvianum OX=4082 PE=2 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 3.8e-63
Identity = 125/160 (78.12%), Postives = 142/160 (88.75%), Query Frame = 0


Query: 1   MDFRIMGMD-SPIFSTLQHVMDLADDGDNKSFNAPTRTYVRDAKAMAATPADIKEYPNSY 60
           MD R++G+D +P+F TL H+M+ A +    S NAP++ YVRDAKAMAATPAD+KEYPNSY
Sbjct: 1   MDLRLLGIDNTPLFHTLHHMMEAAGE---DSVNAPSKIYVRDAKAMAATPADVKEYPNSY 60

Query: 61  VFVVDMPGLKVGDIKVQVEDDNVLLISGERKREEDKEGANAAKYVRMERRVGKFMRKFVL 120
           VFVVDMPGLK GDIKVQVE+DNVLLISGERKREE+KEG   AK++RMERRVGKFMRKF L
Sbjct: 61  VFVVDMPGLKSGDIKVQVEEDNVLLISGERKREEEKEG---AKFIRMERRVGKFMRKFSL 120

Query: 121 PENANTDAISAVCQDGVLTVTVQKLPPPQPKKPKIVEVKV 160
           PENANTDAISAVCQDGVLTVTVQKLPPP+PKKPK +EVKV
Sbjct: 121 PENANTDAISAVCQDGVLTVTVQKLPPPEPKKPKTIEVKV 154

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BLAST of MC11g_new0086 vs. ExPASy Swiss-Prot
Match: P19242 (17.1 kDa class II heat shock protein OS=Pisum sativum OX=3888 GN=HSP17.7 PE=1 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 1.1e-62
Identity = 118/154 (76.62%), Postives = 137/154 (88.96%), Query Frame = 0


Query: 6   MGMDSPIFSTLQHVMDLADDGDNKSFNAPTRTYVRDAKAMAATPADIKEYPNSYVFVVDM 65
           M +DSP+F+TL H+MDL DD   K+ NAPTRTYVRDAKAMAATPAD+KE+PNSYVF+VDM
Sbjct: 1   MDLDSPLFNTLHHIMDLTDDTTEKNLNAPTRTYVRDAKAMAATPADVKEHPNSYVFMVDM 60

Query: 66  PGLKVGDIKVQVEDDNVLLISGERKREEDKEGANAAKYVRMERRVGKFMRKFVLPENANT 125
           PG+K GDIKVQVED+NVLLISGERKREE+KEG    KY++MERR+GK MRKFVLPENAN 
Sbjct: 61  PGVKSGDIKVQVEDENVLLISGERKREEEKEG---VKYLKMERRIGKLMRKFVLPENANI 120

Query: 126 DAISAVCQDGVLTVTVQKLPPPQPKKPKIVEVKV 160
           +AISA+ QDGVLTVTV KLPPP+PKKPK ++VKV
Sbjct: 121 EAISAISQDGVLTVTVNKLPPPEPKKPKTIQVKV 151

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BLAST of MC11g_new0086 vs. ExPASy Swiss-Prot
Match: Q01544 (17.2 kDa class II heat shock protein OS=Ipomoea nil OX=35883 GN=SHSP-1 PE=2 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 9.1e-57
Identity = 112/160 (70.00%), Postives = 134/160 (83.75%), Query Frame = 0


Query: 1   MDFRIMGMDSPIFSTLQHVMDLADDGDNKSFNAPTRTYVRDAKAMAATPADIKEYPNSYV 60
           MD R+MG D P+F    H+MD A D  + + +AP+RT++ DAKAMAATPAD+KEYPNSYV
Sbjct: 1   MDLRLMGFDHPLF---HHIMDYAGDDKSSNSSAPSRTFMLDAKAMAATPADVKEYPNSYV 60

Query: 61  FVVDMPGLKVGDIKVQVEDDNVLLISGERKRE-EDKEGANAAKYVRMERRVGKFMRKFVL 120
           F++DMPGLK GDIKVQV+ DNVL ISGERKRE E+KEG   AKYVRMERRVGK MRKFVL
Sbjct: 61  FIIDMPGLKSGDIKVQVDGDNVLSISGERKREAEEKEG---AKYVRMERRVGKLMRKFVL 120

Query: 121 PENANTDAISAVCQDGVLTVTVQKLPPPQPKKPKIVEVKV 160
           PENAN + I+AVCQDGVLTVTV+ +PPP+PKKP+ +EVK+
Sbjct: 121 PENANKEKITAVCQDGVLTVTVENVPPPEPKKPRTIEVKI 154

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BLAST of MC11g_new0086 vs. ExPASy Swiss-Prot
Match: P46516 (17.9 kDa class II heat shock protein OS=Helianthus annuus OX=4232 GN=HSP17.9 PE=2 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 7.2e-54
Identity = 109/158 (68.99%), Postives = 132/158 (83.54%), Query Frame = 0


Query: 5   IMGMDSPIFSTLQHVMDLADDG--DNKSFNA-PTRTYVRDAKAMAATPADIKEYPNSYVF 64
           +MG D P+   L ++++  DD    NKS N+ P+R YVRDA+AMAATPAD+KE PNSYVF
Sbjct: 6   LMGFD-PLLRNLHYILEATDDNTTGNKSNNSGPSRAYVRDARAMAATPADVKECPNSYVF 65

Query: 65  VVDMPGLKVGDIKVQVEDDNVLLISGERKREEDKEGANAAKYVRMERRVGKFMRKFVLPE 124
           +VDMPGLK GDIKVQVE DNVL+ISG+R REE+KEG    KYVRMERR+GKFM+KF LPE
Sbjct: 66  IVDMPGLKSGDIKVQVERDNVLVISGKRNREEEKEG---VKYVRMERRMGKFMKKFALPE 125

Query: 125 NANTDAISAVCQDGVLTVTVQKLPPPQPKKPKIVEVKV 160
           +ANTD ISA+CQDGVLTVTV+KLPPP+PKKPK ++V+V
Sbjct: 126 DANTDKISAICQDGVLTVTVEKLPPPEPKKPKTIQVQV 159

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BLAST of MC11g_new0086 vs. NCBI nr
Match: XP_022135183.1 (LOW QUALITY PROTEIN: 17.9 kDa class II heat shock protein-like [Momordica charantia])

HSP 1 Score: 290 bits (743), Expect = 2.26e-98
Identity = 154/167 (92.22%), Postives = 154/167 (92.22%), Query Frame = 0


Query: 1   MDFRIMGMDSPIFSTLQHVMDLADDGDNKSFNAPTRTY-------VRDAKAMAATPADIK 60
           MDFRIMGMDSPIFSTLQHVMDLADDGDNKSFN             VRDAKAMAATPADIK
Sbjct: 1   MDFRIMGMDSPIFSTLQHVMDLADDGDNKSFNXXXXXXXXXXXXXVRDAKAMAATPADIK 60

Query: 61  EYPNSYVFVVDMPGLKVGDIKVQVEDDNVLLISGERKREEDKEGANAAKYVRMERRVGKF 120
           EYPNSYVFVVDMPGLKVGDIKVQVEDDNVLLISGERKREEDKEGANAAKYVRMERRVGKF
Sbjct: 61  EYPNSYVFVVDMPGLKVGDIKVQVEDDNVLLISGERKREEDKEGANAAKYVRMERRVGKF 120

Query: 121 MRKFVLPENANTDAISAVCQDGVLTVTVQKLPPPQPKKPKIVEVKVN 160
           MRKFVLPENANTDAISAVCQDGVLTVTVQKLPPPQPKKPKIVEVKVN
Sbjct: 121 MRKFVLPENANTDAISAVCQDGVLTVTVQKLPPPQPKKPKIVEVKVN 167

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BLAST of MC11g_new0086 vs. NCBI nr
Match: ADU55791.1 (HSP17.4 [Citrullus lanatus])

HSP 1 Score: 280 bits (716), Expect = 2.03e-94
Identity = 147/160 (91.88%), Postives = 153/160 (95.62%), Query Frame = 0


Query: 1   MDFRIMGMDSPIFSTLQHVMDLADDGDNKSFNAPTRTYVRDAKAMAATPADIKEYPNSYV 60
           MD RIMG+DSPIFSTLQHVMDLAD+ D KSF+APTRTYVRDAKAMAATPAD+KEYPNSYV
Sbjct: 1   MDLRIMGLDSPIFSTLQHVMDLADEAD-KSFSAPTRTYVRDAKAMAATPADVKEYPNSYV 60

Query: 61  FVVDMPGLKVGDIKVQVEDDNVLLISGERKREEDKEGANAAKYVRMERRVGKFMRKFVLP 120
           FVVDMPGLKVGDIKVQVEDDNVLLISGERKREE+KEGA   KYVRMERRVGK MRKFVLP
Sbjct: 61  FVVDMPGLKVGDIKVQVEDDNVLLISGERKREEEKEGA---KYVRMERRVGKLMRKFVLP 120

Query: 121 ENANTDAISAVCQDGVLTVTVQKLPPPQPKKPKIVEVKVN 160
           ENANTDAISAVCQDGVLTVTVQKLPPP+PKKPKIVEVKVN
Sbjct: 121 ENANTDAISAVCQDGVLTVTVQKLPPPEPKKPKIVEVKVN 156

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BLAST of MC11g_new0086 vs. NCBI nr
Match: XP_022936723.1 (17.3 kDa class II heat shock protein-like [Cucurbita moschata] >XP_022977130.1 17.3 kDa class II heat shock protein-like [Cucurbita maxima])

HSP 1 Score: 278 bits (711), Expect = 1.17e-93
Identity = 143/160 (89.38%), Postives = 153/160 (95.62%), Query Frame = 0


Query: 1   MDFRIMGMDSPIFSTLQHVMDLADDGDNKSFNAPTRTYVRDAKAMAATPADIKEYPNSYV 60
           MD R+MG+DSP+FSTLQHVMD ADD D KSF+APTRTYVRDAKAMAATPAD+KEYPNSYV
Sbjct: 1   MDLRVMGLDSPLFSTLQHVMDFADDAD-KSFSAPTRTYVRDAKAMAATPADVKEYPNSYV 60

Query: 61  FVVDMPGLKVGDIKVQVEDDNVLLISGERKREEDKEGANAAKYVRMERRVGKFMRKFVLP 120
           FVVDMPGLKVGDIKVQVE+DNVLL+SGERKREE+KEGA   KYVRMERRVGKFMRKFVLP
Sbjct: 61  FVVDMPGLKVGDIKVQVEEDNVLLVSGERKREEEKEGA---KYVRMERRVGKFMRKFVLP 120

Query: 121 ENANTDAISAVCQDGVLTVTVQKLPPPQPKKPKIVEVKVN 160
           ENANTDAI+AVCQDGVLTVTVQKLPPP+PKKPKIVEVKVN
Sbjct: 121 ENANTDAITAVCQDGVLTVTVQKLPPPEPKKPKIVEVKVN 156

[Close]

BLAST of MC11g_new0086 vs. NCBI nr
Match: XP_022941229.1 (17.1 kDa class II heat shock protein-like [Cucurbita moschata] >XP_023524413.1 17.1 kDa class II heat shock protein-like [Cucurbita pepo subsp. pepo] >KAG6608349.1 17.9 kDa class II heat shock protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 276 bits (707), Expect = 4.78e-93
Identity = 145/160 (90.62%), Postives = 150/160 (93.75%), Query Frame = 0


Query: 1   MDFRIMGMDSPIFSTLQHVMDLADDGDNKSFNAPTRTYVRDAKAMAATPADIKEYPNSYV 60
           MD RIMG+DSPIFSTL HVMDL D+ D KSFNAPTRTYVRDAKAMAATPADIKEYPNSYV
Sbjct: 1   MDLRIMGLDSPIFSTLHHVMDLVDEAD-KSFNAPTRTYVRDAKAMAATPADIKEYPNSYV 60

Query: 61  FVVDMPGLKVGDIKVQVEDDNVLLISGERKREEDKEGANAAKYVRMERRVGKFMRKFVLP 120
           FVVDMPGLKVGDIKVQVEDDNVLLISGERKREE+KEGA   KYVRMERRVGK MRKFVLP
Sbjct: 61  FVVDMPGLKVGDIKVQVEDDNVLLISGERKREEEKEGA---KYVRMERRVGKLMRKFVLP 120

Query: 121 ENANTDAISAVCQDGVLTVTVQKLPPPQPKKPKIVEVKVN 160
           ENAN DAISAVCQDGVLTVTVQKLPPP+PKKPK+VEVKVN
Sbjct: 121 ENANVDAISAVCQDGVLTVTVQKLPPPEPKKPKVVEVKVN 156

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BLAST of MC11g_new0086 vs. NCBI nr
Match: XP_038898182.1 (17.9 kDa class II heat shock protein-like [Benincasa hispida])

HSP 1 Score: 276 bits (705), Expect = 9.64e-93
Identity = 145/160 (90.62%), Postives = 152/160 (95.00%), Query Frame = 0


Query: 1   MDFRIMGMDSPIFSTLQHVMDLADDGDNKSFNAPTRTYVRDAKAMAATPADIKEYPNSYV 60
           MD RIMG+DSPIFSTLQHVMDLAD+ D KSF+APTRTYVRDAKAMAATPAD+KEY NSYV
Sbjct: 1   MDLRIMGLDSPIFSTLQHVMDLADEAD-KSFSAPTRTYVRDAKAMAATPADVKEYTNSYV 60

Query: 61  FVVDMPGLKVGDIKVQVEDDNVLLISGERKREEDKEGANAAKYVRMERRVGKFMRKFVLP 120
           FVVDMPGLKVGDIKVQVEDDNVLLISGERKREE+KEGA   KYVRMERRVGK MRKFVLP
Sbjct: 61  FVVDMPGLKVGDIKVQVEDDNVLLISGERKREEEKEGA---KYVRMERRVGKLMRKFVLP 120

Query: 121 ENANTDAISAVCQDGVLTVTVQKLPPPQPKKPKIVEVKVN 160
           +NANTDAISAVCQDGVLTVTVQKLPPP+PKKPKIVEVKVN
Sbjct: 121 DNANTDAISAVCQDGVLTVTVQKLPPPEPKKPKIVEVKVN 156

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BLAST of MC11g_new0086 vs. ExPASy TrEMBL
Match: A0A6J1C436 (LOW QUALITY PROTEIN: 17.9 kDa class II heat shock protein-like OS=Momordica charantia OX=3673 GN=LOC111007209 PE=3 SV=1)

HSP 1 Score: 290 bits (743), Expect = 1.10e-98
Identity = 154/167 (92.22%), Postives = 154/167 (92.22%), Query Frame = 0


Query: 1   MDFRIMGMDSPIFSTLQHVMDLADDGDNKSFNAPTRTY-------VRDAKAMAATPADIK 60
           MDFRIMGMDSPIFSTLQHVMDLADDGDNKSFN             VRDAKAMAATPADIK
Sbjct: 1   MDFRIMGMDSPIFSTLQHVMDLADDGDNKSFNXXXXXXXXXXXXXVRDAKAMAATPADIK 60

Query: 61  EYPNSYVFVVDMPGLKVGDIKVQVEDDNVLLISGERKREEDKEGANAAKYVRMERRVGKF 120
           EYPNSYVFVVDMPGLKVGDIKVQVEDDNVLLISGERKREEDKEGANAAKYVRMERRVGKF
Sbjct: 61  EYPNSYVFVVDMPGLKVGDIKVQVEDDNVLLISGERKREEDKEGANAAKYVRMERRVGKF 120

Query: 121 MRKFVLPENANTDAISAVCQDGVLTVTVQKLPPPQPKKPKIVEVKVN 160
           MRKFVLPENANTDAISAVCQDGVLTVTVQKLPPPQPKKPKIVEVKVN
Sbjct: 121 MRKFVLPENANTDAISAVCQDGVLTVTVQKLPPPQPKKPKIVEVKVN 167

[Close]

BLAST of MC11g_new0086 vs. ExPASy TrEMBL
Match: H6TB44 (HSP17.4 OS=Citrullus lanatus OX=3654 PE=2 SV=1)

HSP 1 Score: 280 bits (716), Expect = 9.82e-95
Identity = 147/160 (91.88%), Postives = 153/160 (95.62%), Query Frame = 0


Query: 1   MDFRIMGMDSPIFSTLQHVMDLADDGDNKSFNAPTRTYVRDAKAMAATPADIKEYPNSYV 60
           MD RIMG+DSPIFSTLQHVMDLAD+ D KSF+APTRTYVRDAKAMAATPAD+KEYPNSYV
Sbjct: 1   MDLRIMGLDSPIFSTLQHVMDLADEAD-KSFSAPTRTYVRDAKAMAATPADVKEYPNSYV 60

Query: 61  FVVDMPGLKVGDIKVQVEDDNVLLISGERKREEDKEGANAAKYVRMERRVGKFMRKFVLP 120
           FVVDMPGLKVGDIKVQVEDDNVLLISGERKREE+KEGA   KYVRMERRVGK MRKFVLP
Sbjct: 61  FVVDMPGLKVGDIKVQVEDDNVLLISGERKREEEKEGA---KYVRMERRVGKLMRKFVLP 120

Query: 121 ENANTDAISAVCQDGVLTVTVQKLPPPQPKKPKIVEVKVN 160
           ENANTDAISAVCQDGVLTVTVQKLPPP+PKKPKIVEVKVN
Sbjct: 121 ENANTDAISAVCQDGVLTVTVQKLPPPEPKKPKIVEVKVN 156

[Close]

BLAST of MC11g_new0086 vs. ExPASy TrEMBL
Match: A0A6J1ILE9 (17.3 kDa class II heat shock protein-like OS=Cucurbita maxima OX=3661 GN=LOC111477291 PE=3 SV=1)

HSP 1 Score: 278 bits (711), Expect = 5.68e-94
Identity = 143/160 (89.38%), Postives = 153/160 (95.62%), Query Frame = 0


Query: 1   MDFRIMGMDSPIFSTLQHVMDLADDGDNKSFNAPTRTYVRDAKAMAATPADIKEYPNSYV 60
           MD R+MG+DSP+FSTLQHVMD ADD D KSF+APTRTYVRDAKAMAATPAD+KEYPNSYV
Sbjct: 1   MDLRVMGLDSPLFSTLQHVMDFADDAD-KSFSAPTRTYVRDAKAMAATPADVKEYPNSYV 60

Query: 61  FVVDMPGLKVGDIKVQVEDDNVLLISGERKREEDKEGANAAKYVRMERRVGKFMRKFVLP 120
           FVVDMPGLKVGDIKVQVE+DNVLL+SGERKREE+KEGA   KYVRMERRVGKFMRKFVLP
Sbjct: 61  FVVDMPGLKVGDIKVQVEEDNVLLVSGERKREEEKEGA---KYVRMERRVGKFMRKFVLP 120

Query: 121 ENANTDAISAVCQDGVLTVTVQKLPPPQPKKPKIVEVKVN 160
           ENANTDAI+AVCQDGVLTVTVQKLPPP+PKKPKIVEVKVN
Sbjct: 121 ENANTDAITAVCQDGVLTVTVQKLPPPEPKKPKIVEVKVN 156

[Close]

BLAST of MC11g_new0086 vs. ExPASy TrEMBL
Match: A0A6J1FE17 (17.3 kDa class II heat shock protein-like OS=Cucurbita moschata OX=3662 GN=LOC111443230 PE=3 SV=1)

HSP 1 Score: 278 bits (711), Expect = 5.68e-94
Identity = 143/160 (89.38%), Postives = 153/160 (95.62%), Query Frame = 0


Query: 1   MDFRIMGMDSPIFSTLQHVMDLADDGDNKSFNAPTRTYVRDAKAMAATPADIKEYPNSYV 60
           MD R+MG+DSP+FSTLQHVMD ADD D KSF+APTRTYVRDAKAMAATPAD+KEYPNSYV
Sbjct: 1   MDLRVMGLDSPLFSTLQHVMDFADDAD-KSFSAPTRTYVRDAKAMAATPADVKEYPNSYV 60

Query: 61  FVVDMPGLKVGDIKVQVEDDNVLLISGERKREEDKEGANAAKYVRMERRVGKFMRKFVLP 120
           FVVDMPGLKVGDIKVQVE+DNVLL+SGERKREE+KEGA   KYVRMERRVGKFMRKFVLP
Sbjct: 61  FVVDMPGLKVGDIKVQVEEDNVLLVSGERKREEEKEGA---KYVRMERRVGKFMRKFVLP 120

Query: 121 ENANTDAISAVCQDGVLTVTVQKLPPPQPKKPKIVEVKVN 160
           ENANTDAI+AVCQDGVLTVTVQKLPPP+PKKPKIVEVKVN
Sbjct: 121 ENANTDAITAVCQDGVLTVTVQKLPPPEPKKPKIVEVKVN 156

[Close]

BLAST of MC11g_new0086 vs. ExPASy TrEMBL
Match: A0A6J1FMP1 (17.1 kDa class II heat shock protein-like OS=Cucurbita moschata OX=3662 GN=LOC111446600 PE=3 SV=1)

HSP 1 Score: 276 bits (707), Expect = 2.31e-93
Identity = 145/160 (90.62%), Postives = 150/160 (93.75%), Query Frame = 0


Query: 1   MDFRIMGMDSPIFSTLQHVMDLADDGDNKSFNAPTRTYVRDAKAMAATPADIKEYPNSYV 60
           MD RIMG+DSPIFSTL HVMDL D+ D KSFNAPTRTYVRDAKAMAATPADIKEYPNSYV
Sbjct: 1   MDLRIMGLDSPIFSTLHHVMDLVDEAD-KSFNAPTRTYVRDAKAMAATPADIKEYPNSYV 60

Query: 61  FVVDMPGLKVGDIKVQVEDDNVLLISGERKREEDKEGANAAKYVRMERRVGKFMRKFVLP 120
           FVVDMPGLKVGDIKVQVEDDNVLLISGERKREE+KEGA   KYVRMERRVGK MRKFVLP
Sbjct: 61  FVVDMPGLKVGDIKVQVEDDNVLLISGERKREEEKEGA---KYVRMERRVGKLMRKFVLP 120

Query: 121 ENANTDAISAVCQDGVLTVTVQKLPPPQPKKPKIVEVKVN 160
           ENAN DAISAVCQDGVLTVTVQKLPPP+PKKPK+VEVKVN
Sbjct: 121 ENANVDAISAVCQDGVLTVTVQKLPPPEPKKPKVVEVKVN 156

[Close]

BLAST of MC11g_new0086 vs. TAIR 10
Match: AT5G12020.1 (17.6 kDa class II heat shock protein )

HSP 1 Score: 192.6 bits (488), Expect = 2.5e-49
Identity = 99/151 (65.56%), Postives = 126/151 (83.44%), Query Frame = 0


Query: 11  PIFSTLQHVMDLADDGDN-KSFNAPTRTYVRDAKAMAATPADIKEYPNSYVFVVDMPGLK 70
           PI S L+ ++++ +D +N K+ N P+R Y+RDAKAMAATPAD+ E+PN+Y FVVDMPG+K
Sbjct: 7   PIISILEDMLEVPEDHNNEKTRNNPSRVYMRDAKAMAATPADVIEHPNAYAFVVDMPGIK 66

Query: 71  VGDIKVQVEDDNVLLISGERKRE-EDKEGANAAKYVRMERRVGKFMRKFVLPENANTDAI 130
             +IKVQVE+DNVL++SGER+RE ++ EG    KYVRMERR+GKFMRKF LPENA+ D I
Sbjct: 67  GDEIKVQVENDNVLVVSGERQRENKENEG---VKYVRMERRMGKFMRKFQLPENADLDKI 126

Query: 131 SAVCQDGVLTVTVQKLPPPQPKKPKIVEVKV 160
           SAVC DGVL VTVQKLPPP+PKKPK ++V+V
Sbjct: 127 SAVCHDGVLKVTVQKLPPPEPKKPKTIQVQV 154

[Close]

BLAST of MC11g_new0086 vs. TAIR 10
Match: AT5G12030.1 (heat shock protein 17.6A )

HSP 1 Score: 178.3 bits (451), Expect = 4.8e-45
Identity = 85/149 (57.05%), Postives = 118/149 (79.19%), Query Frame = 0


Query: 11  PIFSTLQHVMDLADDGDNKSFNAPTRTYVRDAKAMAATPADIKEYPNSYVFVVDMPGLKV 70
           PIFS L+ +++  ++   K+ N P+R Y+RDAKAMAATPAD+ E+P++YVF VDMPG+K 
Sbjct: 9   PIFSILEDMLEAPEEQTEKTRNNPSRAYMRDAKAMAATPADVIEHPDAYVFAVDMPGIKG 68

Query: 71  GDIKVQVEDDNVLLISGERKREEDKEGANAAKYVRMERRVGKFMRKFVLPENANTDAISA 130
            +I+VQ+E++NVL++SG  KR+ D +     K+VRMERR+GKFMRKF LP+NA+ + ISA
Sbjct: 69  DEIQVQIENENVLVVSG--KRQRDNKENEGVKFVRMERRMGKFMRKFQLPDNADLEKISA 128

Query: 131 VCQDGVLTVTVQKLPPPQPKKPKIVEVKV 160
            C DGVL VT+ KLPPP+PKKPK ++V+V
Sbjct: 129 ACNDGVLKVTIPKLPPPEPKKPKTIQVQV 155

[Close]

BLAST of MC11g_new0086 vs. TAIR 10
Match: AT1G54050.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 99.8 bits (247), Expect = 2.2e-21
Identity = 53/115 (46.09%), Postives = 83/115 (72.17%), Query Frame = 0


Query: 49  PADIKEYPNSYVFVVDMPGLKVGDIKVQVEDDNVLLI--SGERKREEDKEGANAAKYVRM 108
           P DI E P  Y+F +D+PG+   DI+V VE++  L+I  +G+RKR++D E    +KY+R+
Sbjct: 43  PIDILESPKEYIFYLDIPGISKSDIQVTVEEERTLVIKSNGKRKRDDD-ESEEGSKYIRL 102

Query: 109 ERRVGK-FMRKFVLPENANTDAISAVCQDGVLTVTVQKLPPPQPKKPKIVEVKVN 161
           ERR+ +  ++KF LPE+A+  +++A  Q+GVLTV ++KL PPQP KPK V++ V+
Sbjct: 103 ERRLAQNLVKKFRLPEDADMASVTAKYQEGVLTVVIKKL-PPQPPKPKTVQIAVS 155

[Close]

BLAST of MC11g_new0086 vs. TAIR 10
Match: AT5G59720.1 (heat shock protein 18.2 )

HSP 1 Score: 99.8 bits (247), Expect = 2.2e-21
Identity = 55/121 (45.45%), Postives = 77/121 (63.64%), Query Frame = 0


Query: 40  RDAKAMAATPADIKEYPNSYVFVVDMPGLKVGDIKVQVEDDNVLLISGERKREEDKEGAN 99
           RD  A      D KE P ++VF  D+PGLK  ++KV+VED NVL ISGER +E +++   
Sbjct: 44  RDVAAFTNARVDWKETPEAHVFKADLPGLKKEEVKVEVEDKNVLQISGERSKENEEKN-- 103

Query: 100 AAKYVRMERRVGKFMRKFVLPENANTDAISAVCQDGVLTVTVQKLPPPQPKKPKIVEVKV 159
             K+ R+ER  GKFMR+F LPENA  + + A  ++GVLTV V K P    KKP++  + +
Sbjct: 104 -DKWHRVERASGKFMRRFRLPENAKMEEVKATMENGVLTVVVPKAP---EKKPQVKSIDI 158

Query: 160 N 161
           +
Sbjct: 164 S 158

[Close]

BLAST of MC11g_new0086 vs. TAIR 10
Match: AT3G46230.1 (heat shock protein 17.4 )

HSP 1 Score: 94.0 bits (232), Expect = 1.2e-19
Identity = 52/126 (41.27%), Postives = 78/126 (61.90%), Query Frame = 0


Query: 35  TRTYVRDAKAMAATPADIKEYPNSYVFVVDMPGLKVGDIKVQVEDDNVLLISGERKREED 94
           T    +D  A      D +E P ++VF  D+PGLK  ++KV+VED N+L ISGER  E +
Sbjct: 36  TNAPAKDVAAFTNAKVDWRETPEAHVFKADVPGLKKEEVKVEVEDGNILQISGERSSENE 95

Query: 95  KEGANAAKYVRMERRVGKFMRKFVLPENANTDAISAVCQDGVLTVTVQKLPPPQPKKPKI 154
           ++   +  + R+ER  GKFMR+F LPENA  + + A  ++GVL+VTV K+   Q  KP++
Sbjct: 96  EK---SDTWHRVERSSGKFMRRFRLPENAKVEEVKASMENGVLSVTVPKV---QESKPEV 155

Query: 155 VEVKVN 161
             V ++
Sbjct: 156 KSVDIS 155

The following BLAST results are available for this feature:

BLAST of MC11g_new0086 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P05477	1.1e-67	78.88	17.9 kDa class II heat shock protein OS=Glycine max OX=3847 GN=HSP17.9-D PE=3 SV...	[more]
O82013	3.8e-63	78.13	17.3 kDa class II heat shock protein OS=Solanum peruvianum OX=4082 PE=2 SV=1	[more]
P19242	1.1e-62	76.62	17.1 kDa class II heat shock protein OS=Pisum sativum OX=3888 GN=HSP17.7 PE=1 SV...	[more]
Q01544	9.1e-57	70.00	17.2 kDa class II heat shock protein OS=Ipomoea nil OX=35883 GN=SHSP-1 PE=2 SV=1	[more]
P46516	7.2e-54	68.99	17.9 kDa class II heat shock protein OS=Helianthus annuus OX=4232 GN=HSP17.9 PE=...	[more]

BLAST of MC11g_new0086 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135183.1	2.26e-98	92.22	LOW QUALITY PROTEIN: 17.9 kDa class II heat shock protein-like [Momordica charan...	[more]
ADU55791.1	2.03e-94	91.88	HSP17.4 [Citrullus lanatus]	[more]
XP_022936723.1	1.17e-93	89.38	17.3 kDa class II heat shock protein-like [Cucurbita moschata] >XP_022977130.1 1...	[more]
XP_022941229.1	4.78e-93	90.63	17.1 kDa class II heat shock protein-like [Cucurbita moschata] >XP_023524413.1 1...	[more]
XP_038898182.1	9.64e-93	90.63	17.9 kDa class II heat shock protein-like [Benincasa hispida]	[more]

BLAST of MC11g_new0086 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C436	1.10e-98	92.22	LOW QUALITY PROTEIN: 17.9 kDa class II heat shock protein-like OS=Momordica char...	[more]
H6TB44	9.82e-95	91.88	HSP17.4 OS=Citrullus lanatus OX=3654 PE=2 SV=1	[more]
A0A6J1ILE9	5.68e-94	89.38	17.3 kDa class II heat shock protein-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1FE17	5.68e-94	89.38	17.3 kDa class II heat shock protein-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1FMP1	2.31e-93	90.63	17.1 kDa class II heat shock protein-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]

BLAST of MC11g_new0086 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G12020.1	2.5e-49	65.56	17.6 kDa class II heat shock protein	[more]
AT5G12030.1	4.8e-45	57.05	heat shock protein 17.6A	[more]
AT1G54050.1	2.2e-21	46.09	HSP20-like chaperones superfamily protein	[more]
AT5G59720.1	2.2e-21	45.45	heat shock protein 18.2	[more]
AT3G46230.1	1.2e-19	41.27	heat shock protein 17.4	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008978	HSP20-like chaperone	GENE3D	2.60.40.790		coord: 6..157 e-value: 8.1E-39 score: 134.7
IPR008978	HSP20-like chaperone	SUPERFAMILY	49764	HSP20-like chaperones	coord: 28..154
IPR002068	Alpha crystallin/Hsp20 domain	PFAM	PF00011	HSP20	coord: 51..157 e-value: 7.5E-29 score: 99.9
IPR002068	Alpha crystallin/Hsp20 domain	PROSITE	PS01031	SHSP	coord: 41..160 score: 28.758341
IPR031107	Small heat shock protein HSP20	PANTHER	PTHR11527	HEAT-SHOCK PROTEIN 20 FAMILY MEMBER	coord: 8..159
None	No IPR available	PANTHER	PTHR11527:SF260	18.0 KDA CLASS II HEAT SHOCK PROTEIN	coord: 8..159

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC11g_new0086.1	MC11g_new0086.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051259	protein complex oligomerization
biological_process	GO:0006457	protein folding
biological_process	GO:0009408	response to heat
biological_process	GO:0042542	response to hydrogen peroxide
biological_process	GO:0009651	response to salt stress
molecular_function	GO:0043621	protein self-association
molecular_function	GO:0051082	unfolded protein binding