MC11g_new0060 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC11g_new0060
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	alpha/beta-Hydrolases superfamily protein
Location	MC11: 1839301 .. 1844080 (-)
RNA-Seq Expression	MC11g_new0060
Synteny	MC11g_new0060

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TAACAAATTAAAAAAATTCTCAATCCTCTTGAGCATTATTGTTGGGTGGGATTACAAAAAAATGGGTCCCGAAGATTACCAATTCAGTAATGATTATCTGATACTGAAGCCTGAGGAAGCAACTCTGCTGGATCTGTTTCTCTTCATACTTCCTTTCGGTTACTTAAGGCTCAGGAAGTTGGTGGATTGCCCCGAAGAAAAAGAAGAGTCTTACAAAAGCTTTGGAAACCGATGGGTTATCTTCATCTCCATTTTGTCCCAGAAATTCCTCCTCGCCATCGCCAATCTGCTCGAAAAGTGGGAGCCGATGCGAGGTACATTTTATAAGGAGGAGTAACATTTATTCAAAAAAGTAATCACGGTTCGATGAAACGAAATGGTTGAATTTCGACACCATAACTCAACTCGTTAAAACATATATACAAAGACTTAATTTCGTTTTATGTTAATTACTTGTATTGTTACCGTATTGCGTGCACATGCATGCAGGAAAATTCTGGCGGGTGCCTGATCATAAAACTTATGCATCGGAAGTGAACTGTAGAGATTGGAAAATAGGTACGAGCATAAAATTACCCAACGAAGACGCTTTCATATATTATGGTGCCGTCACAGTTATGGCTTCGAATTTGGCTTACCAAGACTATTCATCTGAACCCTCCGTGGTCGAGACCGTCGTCAGTTCCTGCTGGAAGGTAAATACAATAATACTTGATTACCTCGTGATCAGTAGAAAGATTCCTATAGATGGGTATATGGCTAAGTATATTATATTCAAATAAATATATGTGCGGTGGTACTGATTCTTCTAACAGTTAGATATGTGTTATATGGCTATCAATAAATAAAGACATCTATTACCATCTCAAAAGTTGATTGCTTGATTCTCATCCTACGACTGTAAAAATTATATATTATAGGTAAAAGTATCAGTTTTACCTTGGATCTTTTTACCTATGAAATTTGACGAAGTTAAACTTTCAATTTAGAATTAATGGACCTTAATTGCTTCAAAAAGGGTGCAATATTTGTCCAATGCTAAGATTTTTATTTTTTATTTTTTAAGAACTGTAAAAAATGGTTATCATGCTAGCTATTGAATGAATTATTTTTTTCGAACAGAAAGAGGCCAAAGGCCTAAGAAACAGGAGAAGGAGCTATTGAATGAATTATGATACGTAGGTTCAAACTTTAGTACAAGAAGAACTGAACTTTTAATGGCTAGATTTTCTTGTTAAAATTTGCGTATATGTTTATATTATTCAATTGTTGATCTTGTGTGTATGACTTCAGATTTAATTTTGTGTTACTTAATTAAGGTTTGATCTTCATTGGAATTAGTGATTTTTATGTTTTTTTTTTTTTTTGAAAAGTTGGTGATTTTTATGCCGTTTTTTGAAAATTAGTGAGAATGAGTAGAGCAAAAGTTGTATATAGAAGGTAAATATTGTAATACTCAGATCTAAATCTATTGTTTAATTTGATAGACTAAAAGTGTACGGTGAAAATTGAAACAAAACTTATACACCTGTTACCTTTTTGACCTGGCTAATTTTTATCTGTCTATTTCTATTGCAAAGTAGATCACTGGATTTTTTAAGTCAATAAAAATATGTCACATCATAAAATCGGTAGAAAATCATATGTGTAGGGAAGTATTTAAAAAAAGTGGATAGGCAGTATTTTACTAAAAAAATTGATTTACTTTGCGAGGTTTAGAGATATAAATTATAATTCATTTCTTAAAAATACTATTTTTTCTAAATATATAATATATCTAACTAAATCGAATTATTAATATTTTGGATGGTAGAGTGCGTGCCATTAACTATGGAGCTATAACTTATTCAAACATAGCTCAGTAAATAAAGGTACTTATTACTATACCATATATCCTAGCATTGTTGAACTCAATAAAATAAAATAAAAATTGAACTATTTTTTAATTGCCTAAAATATAAAAAACCATAAGATGCAATTTCAATTATAATCATGGTTGAAATATTTGGGGATGGTGATAAAATGATGATGTGTTATTATTTTTAACGCAGATGAATCTACTTGGATGCTACAATTTTTGGAATGGTAAGATTTTGGACTTCTATCGTATATATTTTTGTCTTTAATATCTTTCTTTACAAATTTAGTTTGTAACCAAGAAGCCGTGAGTTCAATCCTCTTCCCACATATTGTACTCATAGGAAAAAAAATAATTCAAAACATTAAAAGTAGATAGATGGAAATAGTATTTATTTCTAAGAAATTCATGAACCCAATTCATATATGCTTATGAAACTGAACAATTTTTCTCTTTAATTTGAACAATATGATCTTCAGATTTCCAGAACAAGGCTTCGACTCAAGCATTTATGTTTCAGAATACCGCGACGGACCCAAACGTAACAGTGGTGGCATTTAGAGGCACCAATAGCATAGATAATATCATGGTAGATTTGAACTTCTCGTGGTGCAATTTTGATCAGCGCATTGGCAACATCCACAGCGGCTTCATGGAAGCTCTGGGCCTCCAAAAGGGCCCCATAGGTTGGCCAACGGAACTCCCCACGCCTGACCACGATTTCGCATATTACTACCTCCGCCAACAACTTAGAGACATTGCCAAATCCAACGACAAGGCAAGGTTCGTGTTCACCGGCCACAGCTTGGGCGGAGCCCTCGCAATCCTGTTTGTCACCATCCTCTCGTACCACAACGAGTCCGATGTGCTCAACAAGCTGCAGGCTGTTTACACCTTCGGCCAACCCAGAGTCGGCAATAGCCAGTTTGCTCAGTTTATGGAGAAAACTTTACAACAATATGCCTTCAAATATTATCGATACGTTTACTCTATGGACTTGGCGCCCAGAATTCCTTTCGATATCTTTGATCAATGGTACGAACACTTTGGCGGATGCGTTTACTTCAACTGCTGCTACAGTGGCAAGGTAATTATATTCTTGGAAGAGTGATAATACTAAAAGAAAAATACTTCCGTTCTAACTAGTGAGGACCTATTTTTATATTGTTAAAGATTTCACGTTGAAAAGATAAGGGACCTTACAATGGTTTATAATAAATATGGGTTACTACTCTCATCTCCAATTGGTTTTGAGATGCAACCCCATGAATATTAATATGGTCGGATCCCATTTTAGACCGGAAGTTCGTTTTAGCGTCTTGGGATCTGATACCATCTTAATATTCATGAGATTTCATCTCAAAACCAATTGACAAAGAGAGTTAACAAAGAGAGGAATAGCTCACGTTTATTATAAGTCATTGTATAGTCTCTACAAACTGATTGCTCATTTAAAAATATATATTTATAAGTCATTGTATGATCTCTACGAACTGATTGCTCATTTAAAAATATATATTTGTTAAAAGAAAAAAGTACAAAGGGTATACAACATCCCATTACCCCAAATTGACTAAGTATTGTCCATTTTATATATAACTATGTAGTAAAAAAAAATTAATTTGTTCAAGACATATTTGCTAAGTGTTTATAGTGACAAAAAAAAAACACTGATTGCAAACTTCCCTGAACAAATTGATTTTTTTACTACCATATTATATATAAAATAAATGGTATAGGTGGGTTTTACAAGTGAATGATAATAGATGGGTATACCCCGTACTTCTAAGACATGAACTATTTTTTTAATTATGTTAATCGGTTTTGAAATGAAATTTATGGGGAGGATCATTTTTAATTAGTTCCCTCTTTTTTAAGGATAGGAAGGAGAATGAATTAATTGTTTTGGGTTGAGATGTGCAGTTTCTGGAGGTGCAGCCAAACAAGAACTACTTCTCGCCGATATGGCTGATACCCAAATACTTGTTCGCATGGTGGGAGCTTATAAGAAGTTTAATGATCCCAATCATCAAGTGTTCCAGCTATTACTTCGAGGGTTTTTCTGCTTTATTGGTTAGGTTGTTTGGGTTGCTTCTTCCAGGGGCCTCGGCTCACATATGTCTCAATTATATTAATTTGATCCGTTGGGGAAAAATACAACAACCAACCAAAGACGTCCAAAAGGCCCTAATTCCTACAAACTATCTCCAAGCTCATCATCAGTAGTACTAAAATCTGGAGAACATGAACATCCCAAATATATAATATATTTGTAATGTGTGCGCTTTTATATTTTAATGTGGGAATAAGGTGTGTTTATTTGTTAGTGTATTTCTTGATGTTATGCAAATCTGACCCACGAGCCAAAACGTTTGGTAACTGCTTTTAAAGACTTTATGTATGTGAGTGGTTCATAATAAAGTTTCGTTCTAATGTGCTTTAAGATTCCTGCTTAATTTCAATCTAATTGAAGTACTATTGTTTTAGTAGCCTAGACTACGTGTCAACCTCACATGTATAATCTTATTACAGAATGATAAGAAGATTCAACCACATCTTTATCAAGTGATTTTGGCAGCTCGACAACTAGAAGCTGAGTTACGTATTACCTTTTGGTCGACTGTCATCAGGTACGAGTGAACTTTAAAGAACACCTGCAAGTGCAAAGACAATCAGGGATGTTCTCTGACAACATGTCGAATTTCCTTGAGATGTCGAAGAACCGTTAGAGTGCTGTCGAACGAAGTTCTGTTGGAAGTTCATCTCTATAAATAGAGATCCACGCCTCCGATATGTGAAACTATTCTCTCTATGAGCAATAGATCAGATTGTGTACTAATTAGATCCTCGGAGTGTTGTCAATACACTTTTTTCGAGCTTTGCAAATCCTTGATTCGCAATTAAATTAAACACTGATGATTA

mRNA sequence

ATGGGTCCCGAAGATTACCAATTCAGTAATGATTATCTGATACTGAAGCCTGAGGAAGCAACTCTGCTGGATCTGTTTCTCTTCATACTTCCTTTCGGTTACTTAAGGCTCAGGAAGTTGGTGGATTGCCCCGAAGAAAAAGAAGAGTCTTACAAAAGCTTTGGAAACCGATGGGTTATCTTCATCTCCATTTTGTCCCAGAAATTCCTCCTCGCCATCGCCAATCTGCTCGAAAAGTGGGAGCCGATGCGAGGAAAATTCTGGCGGGTGCCTGATCATAAAACTTATGCATCGGAAGTGAACTGTAGAGATTGGAAAATAGGTACGAGCATAAAATTACCCAACGAAGACGCTTTCATATATTATGGTGCCGTCACAGTTATGGCTTCGAATTTGGCTTACCAAGACTATTCATCTGAACCCTCCGTGGTCGAGACCGTCGTCAGTTCCTGCTGGAAGATGAATCTACTTGGATGCTACAATTTTTGGAATGATTTCCAGAACAAGGCTTCGACTCAAGCATTTATGTTTCAGAATACCGCGACGGACCCAAACGTAACAGTGGTGGCATTTAGAGGCACCAATAGCATAGATAATATCATGGTAGATTTGAACTTCTCGTGGTGCAATTTTGATCAGCGCATTGGCAACATCCACAGCGGCTTCATGGAAGCTCTGGGCCTCCAAAAGGGCCCCATAGGTTGGCCAACGGAACTCCCCACGCCTGACCACGATTTCGCATATTACTACCTCCGCCAACAACTTAGAGACATTGCCAAATCCAACGACAAGGCAAGGTTCGTGTTCACCGGCCACAGCTTGGGCGGAGCCCTCGCAATCCTGTTTGTCACCATCCTCTCGTACCACAACGAGTCCGATGTGCTCAACAAGCTGCAGGCTGTTTACACCTTCGGCCAACCCAGAGTCGGCAATAGCCAGTTTGCTCAGTTTATGGAGAAAACTTTACAACAATATGCCTTCAAATATTATCGATACGTTTACTCTATGGACTTGGCGCCCAGAATTCCTTTCGATATCTTTGATCAATGGTACGAACACTTTGGCGGATGCGTTTACTTCAACTGCTGCTACAGTGGCAAGTTTCTGGAGGTGCAGCCAAACAAGAACTACTTCTCGCCGATATGGCTGATACCCAAATACTTGTTCGCATGGTGGGAGCTTATAAGAAGTTTAATGATCCCAATCATCAAGTGTTCCAGCTATTACTTCGAGGGTTTTTCTGCTTTATTGGTTAGGTTGTTTGGGTTGCTTCTTCCAGGGGCCTCGGCTCACATATGTCTCAATTATATTAATTTGATCCGTTGGGGAAAAATACAACAACCAACCAAAGACGTCCAAAAGGCCCTAATTCCTACAAACTATCTCCAAGCTCATCATCAGTAG

Coding sequence (CDS)

ATGGGTCCCGAAGATTACCAATTCAGTAATGATTATCTGATACTGAAGCCTGAGGAAGCAACTCTGCTGGATCTGTTTCTCTTCATACTTCCTTTCGGTTACTTAAGGCTCAGGAAGTTGGTGGATTGCCCCGAAGAAAAAGAAGAGTCTTACAAAAGCTTTGGAAACCGATGGGTTATCTTCATCTCCATTTTGTCCCAGAAATTCCTCCTCGCCATCGCCAATCTGCTCGAAAAGTGGGAGCCGATGCGAGGAAAATTCTGGCGGGTGCCTGATCATAAAACTTATGCATCGGAAGTGAACTGTAGAGATTGGAAAATAGGTACGAGCATAAAATTACCCAACGAAGACGCTTTCATATATTATGGTGCCGTCACAGTTATGGCTTCGAATTTGGCTTACCAAGACTATTCATCTGAACCCTCCGTGGTCGAGACCGTCGTCAGTTCCTGCTGGAAGATGAATCTACTTGGATGCTACAATTTTTGGAATGATTTCCAGAACAAGGCTTCGACTCAAGCATTTATGTTTCAGAATACCGCGACGGACCCAAACGTAACAGTGGTGGCATTTAGAGGCACCAATAGCATAGATAATATCATGGTAGATTTGAACTTCTCGTGGTGCAATTTTGATCAGCGCATTGGCAACATCCACAGCGGCTTCATGGAAGCTCTGGGCCTCCAAAAGGGCCCCATAGGTTGGCCAACGGAACTCCCCACGCCTGACCACGATTTCGCATATTACTACCTCCGCCAACAACTTAGAGACATTGCCAAATCCAACGACAAGGCAAGGTTCGTGTTCACCGGCCACAGCTTGGGCGGAGCCCTCGCAATCCTGTTTGTCACCATCCTCTCGTACCACAACGAGTCCGATGTGCTCAACAAGCTGCAGGCTGTTTACACCTTCGGCCAACCCAGAGTCGGCAATAGCCAGTTTGCTCAGTTTATGGAGAAAACTTTACAACAATATGCCTTCAAATATTATCGATACGTTTACTCTATGGACTTGGCGCCCAGAATTCCTTTCGATATCTTTGATCAATGGTACGAACACTTTGGCGGATGCGTTTACTTCAACTGCTGCTACAGTGGCAAGTTTCTGGAGGTGCAGCCAAACAAGAACTACTTCTCGCCGATATGGCTGATACCCAAATACTTGTTCGCATGGTGGGAGCTTATAAGAAGTTTAATGATCCCAATCATCAAGTGTTCCAGCTATTACTTCGAGGGTTTTTCTGCTTTATTGGTTAGGTTGTTTGGGTTGCTTCTTCCAGGGGCCTCGGCTCACATATGTCTCAATTATATTAATTTGATCCGTTGGGGAAAAATACAACAACCAACCAAAGACGTCCAAAAGGCCCTAATTCCTACAAACTATCTCCAAGCTCATCATCAGTAG

Protein sequence

MGPEDYQFSNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEKEESYKSFGNRWVIFISILSQKFLLAIANLLEKWEPMRGKFWRVPDHKTYASEVNCRDWKIGTSIKLPNEDAFIYYGAVTVMASNLAYQDYSSEPSVVETVVSSCWKMNLLGCYNFWNDFQNKASTQAFMFQNTATDPNVTVVAFRGTNSIDNIMVDLNFSWCNFDQRIGNIHSGFMEALGLQKGPIGWPTELPTPDHDFAYYYLRQQLRDIAKSNDKARFVFTGHSLGGALAILFVTILSYHNESDVLNKLQAVYTFGQPRVGNSQFAQFMEKTLQQYAFKYYRYVYSMDLAPRIPFDIFDQWYEHFGGCVYFNCCYSGKFLEVQPNKNYFSPIWLIPKYLFAWWELIRSLMIPIIKCSSYYFEGFSALLVRLFGLLLPGASAHICLNYINLIRWGKIQQPTKDVQKALIPTNYLQAHHQ

Homology

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BLAST of MC11g_new0060 vs. ExPASy Swiss-Prot
Match: A0A1S3ZP85 (Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 6.1e-61
Identity = 140/378 (37.04%), Postives = 197/378 (52.12%), Query Frame = 0


Query: 127 VMASNLAYQDYSSEPSVVETVVSSCWKMNLLGCYNFWNDFQNKASTQAFMFQNTATDPNV 186
           +MAS LAY++      VV  VV+  WKM+ +  YN WNDF+ + STQ F+  +   D N+
Sbjct: 192 MMASKLAYEN----EKVVRNVVNLHWKMHFVDFYNCWNDFEKEMSTQVFLLCDKPKDANL 251

Query: 187 TVVAFRGTN--SIDNIMVDLNFSWCNFDQRIGNIHSGFMEALGLQK-------------- 246
            +V+FRGT     D+ + D ++SW     ++G +H GF+EALGL                
Sbjct: 252 ILVSFRGTEPFDADDWITDFDYSWYEI-PKLGKVHMGFLEALGLGNRTNASTFHEQLFVN 311

Query: 247 ----------------------------------GPIGWPTELPTPDHD----------F 306
                                               +   +E PT               
Sbjct: 312 NLKFANLENVHATIPPSESSKSSTSFSDSDAHTGSDLSSDSERPTDTRKKKFRLEIPERT 371

Query: 307 AYYYLRQQLRDIAKSNDKARFVFTGHSLGGALAILFVTILSYHNESDVLNKLQAVYTFGQ 366
           AYY +R +L+ + K +  A+FV TGHSLGGALAILF  +L  H E DV+ +L  +YT+GQ
Sbjct: 372 AYYVVRSKLKRLLKEHKNAKFVVTGHSLGGALAILFPAVLVLHEEVDVMERLLGIYTYGQ 431

Query: 367 PRVGNSQFAQFMEKTLQQYAFKYYRYVYSMDLAPRIPFDIFDQWYEHFGGCVYFNCCYSG 426
           PRVGN Q  +FME  L+    KY+R VY  DL PR+P+D     ++HFG C Y+N  Y  
Sbjct: 432 PRVGNRQLGRFMEAHLEHPVPKYFRVVYCNDLVPRLPYDNKTFLFKHFGICQYYNSLYIE 491

Query: 427 KFLEVQPNKNYFSPIWLIPKYLFAWWELIRSLMIPIIKCSSYYFEGFSALLVRLFGLLLP 445
           + +  +PN NYF   +L+P YL A WELIRS  +  +   S Y E + ++++R  GL LP
Sbjct: 492 QNINEEPNPNYFGMRFLVPLYLNAGWELIRSFTMGYM-YGSEYEECWESVMLRALGLFLP 551

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BLAST of MC11g_new0060 vs. ExPASy Swiss-Prot
Match: F4JFU8 (Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 1.5e-59
Identity = 164/511 (32.09%), Postives = 250/511 (48.92%), Query Frame = 0


Query: 2   GPEDYQFSNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEKEES-----YKSFGN 61
           G    Q SN YLI++P     +DLF + +        K ++ P+ +E S      ++  +
Sbjct: 10  GSNPGQVSN-YLIVRPHRGGYIDLFRYGVRDDQTSKAKFLEMPDNREWSTITIDEEAEDH 69

Query: 62  RWVIFISILSQKFLLAI--------------ANLLEKWEPMRGKFWRVPDHK-------- 121
           RWVI +SIL +K +  +               NL        G   R+   K        
Sbjct: 70  RWVIVVSILVRKIIRLLRTPMEFTGFVVDFFLNLFSANGGFFGLLLRLIQAKVVIPERGS 129

Query: 122 -TYASEVNCRDWKIGTSIK------LPNEDAF------IYYGA-----VTVMASNLAYQD 181
            T+ S +   D +I    +      L   D+       I  G+     + VMAS LAY++
Sbjct: 130 VTFVSTIGQLDGRISLYKEWNFVEHLEGIDSVDSGRVKIELGSRGLMDLCVMASKLAYEN 189

Query: 182 YSSEPSVVETVVSSCWKMNLLGCYNFWNDFQNKASTQAFMFQNTATDPNVTVVAFRGTN- 241
                 VVE VV   WKMNL+   + WND+Q + STQ F+F +   D N+ V++FRGT  
Sbjct: 190 ----AKVVENVVDLHWKMNLVEFLDCWNDYQKQMSTQVFVFTDKQKDANLIVISFRGTEP 249

Query: 242 -SIDNIMVDLNFSWCNFDQRIGNIHSGFMEALGLQKGPIGWPTELPTPDHDF-------- 301
              D+   D ++SW      +G +H GF+EA+GL     G   +  T  ++         
Sbjct: 250 FDADDWGTDFDYSWYEV-PNVGKLHMGFLEAMGL-----GNRDDTTTFHYNLFEQTSSEE 309

Query: 302 --------------AYYYLRQQLRDIAKSNDKARFVFTGHSLGGALAILFVTILSYHNES 361
                         AYY +R  L+ +   ++ ARFV TGHSLGGALAILF T+L  + E+
Sbjct: 310 ENSKKNLLDMVERSAYYAVRVILKRLLSEHENARFVVTGHSLGGALAILFPTLLVLNEET 369

Query: 362 DVLNKLQAVYTFGQPRVGNSQFAQFMEKTLQQYAFKYYRYVYSMDLAPRIPFDIFDQWYE 421
           +++ +L  VYTFGQPR+GN +   FM+  L Q   +Y+R VY  D+ PR+P+D     Y+
Sbjct: 370 EIMKRLLGVYTFGQPRIGNREVGLFMKAQLNQPVDRYFRVVYCNDIVPRLPYDDKTFLYK 429

Query: 422 HFGGCVYFNCCYSGKFLEVQPNKNYFSPIWLIPKYLFAWWELIRSLMIPIIKCSSYYFEG 444
           HFG C++++  Y+    E +P+ N +   + I  ++ A WEL+R L +       Y  EG
Sbjct: 430 HFGLCLFYDSFYNETKAEDEPDPNPYGLRYKILGHVIAVWELVRGLTMGYTHGPDYK-EG 489

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BLAST of MC11g_new0060 vs. ExPASy Swiss-Prot
Match: Q5VKJ7 (Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 8.2e-58
Identity = 135/344 (39.24%), Postives = 192/344 (55.81%), Query Frame = 0


Query: 127 VMASNLAYQDYSSEPSVVETVVSSCWKMNLLGCYNFWNDFQNKASTQAFMFQNTATDPNV 186
           +MAS LAY++      VVE VV+  WKM+ +  Y   N FQ+  +T AF+F +   D N+
Sbjct: 174 IMASKLAYEN----TKVVERVVAEHWKMHFVADYGGMNYFQDARNTHAFIFCDKPKDANL 233

Query: 187 TVVAFRGTN--SIDNIMVDLNFSWCNFDQRIGNIHSGFMEALGL--------------QK 246
            V++FRGT   SI N   D +FS        G++H GF+EA+GL               K
Sbjct: 234 IVISFRGTGPFSIPNWCTDFDFSLVGLGD-AGSVHVGFLEAMGLGHRNSISSFETSINTK 293

Query: 247 GPIGWPTEL-----PTPDH-----DFAYYYLRQQLRDIAKSNDKARFVFTGHSLGGALAI 306
            P G  TEL       PDH     D  Y+     L+ + K +  A+FV TGHSLGGALAI
Sbjct: 294 SP-GSITELRKESEMAPDHLVWAYDGVYFLAASTLKGLLKDHKNAKFVVTGHSLGGALAI 353

Query: 307 LFVTILSYHNESDVLNKLQAVYTFGQPRVGNSQFAQFMEKTLQQYAFKYYRYVYSMDLAP 366
           LF  IL    E++VL++L  VYTFGQPR+GN     FM+  L     +Y+R VY  D+ P
Sbjct: 354 LFTCILEIQQETEVLDRLLNVYTFGQPRIGNYNLGYFMQNRLNFPERRYFRVVYCNDMVP 413

Query: 367 RIPFDIFDQWYEHFGGCVYFNCCYSGKFLEVQPNKNYFSPIWLIPKYLFAWWELIRSLMI 426
           R+PFD     +EHFG C+Y++  + G F + +P++N F     I  ++ AWWEL RS ++
Sbjct: 414 RVPFDDVFFTFEHFGTCIYYDSRFFGYFTKEEPSRNPFGIENAISAHITAWWELWRSFIL 473

Query: 427 PIIKCSSYYFEGFSALLVRLFGLLLPGASAHICLNYINLIRWGK 445
             +   + Y E + + + R+ GL LPG +AH  +NY+N +R G+
Sbjct: 474 NHV-YGAEYKETWESRMFRILGLFLPGVAAHSPVNYVNSVRLGR 510

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BLAST of MC11g_new0060 vs. ExPASy Swiss-Prot
Match: O59952 (Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 4.4e-11
Identity = 54/156 (34.62%), Postives = 78/156 (50.00%), Query Frame = 0


Query: 188 VVAFRGTNSIDNIMVDLNFSWCNFDQRIGNIHSGFMEALGLQKGPIGWPTELPTPDHDFA 247
           V++FRG+ SI+N + +LNF      + I +I SG     G       W +   T      
Sbjct: 99  VLSFRGSRSIENWIGNLNFDL----KEINDICSGCRGHDGFTS---SWRSVADT------ 158

Query: 248 YYYLRQQLRDIAKSNDKARFVFTGHSLGGALAILFVTILSYHNESDVLNKLQAVYTFGQP 307
              LRQ++ D  + +   R VFTGHSLGGALA +    L   N  D+      V+++G P
Sbjct: 159 ---LRQKVEDAVREHPDYRVVFTGHSLGGALATVAGADLR-GNGYDI-----DVFSYGAP 218

Query: 308 RVGNSQFAQFMEKTLQQYAFKYYRYVYSMDLAPRIP 344
           RVGN  FA+F+     Q     YR  ++ D+ PR+P
Sbjct: 219 RVGNRAFAEFL---TVQTGGTLYRITHTNDIVPRLP 229

[Close]

BLAST of MC11g_new0060 vs. ExPASy Swiss-Prot
Match: B8NIB8 (Probable feruloyl esterase A OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=faeA PE=3 SV=2)

HSP 1 Score: 63.9 bits (154), Expect = 5.4e-09
Identity = 55/177 (31.07%), Postives = 81/177 (45.76%), Query Frame = 0


Query: 188 VVAFRGTNSIDNIMVDLNFSWCNFD---QRIG-NIHSGFMEALGLQKGPIGWPTELPTPD 247
           +  FRGT S  N+ +D N++   FD   Q  G  +H G+          +GW +      
Sbjct: 83  ITVFRGTGSDTNLQLDTNYTQAPFDTLPQCSGCAVHGGYY---------VGWISV----- 142

Query: 248 HDFAYYYLRQQLRDIAKSNDKARFVFTGHSLGGALAILFVTILS--YHNESDVLNKLQAV 307
            D     ++QQ    A        V TGHSLG ++A +    LS  Y+N +        V
Sbjct: 143 KDQVEGLVQQQ----ASQYPDYSLVITGHSLGASMAAITAAQLSATYNNIT--------V 202

Query: 308 YTFGQPRVGNSQFAQFMEKTLQQY---AFKYYRYVYSMDLAPRIPFDIFDQWYEHFG 356
           YTFG+PR GN  +A ++++T Q     A K+YR  ++ D  P +P     Q Y H G
Sbjct: 203 YTFGEPRTGNQAYASYVDETFQATNPDATKFYRVTHTNDGIPNLP--PTSQGYVHHG 231

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BLAST of MC11g_new0060 vs. NCBI nr
Match: XP_022135571.1 (uncharacterized protein LOC111007483 isoform X2 [Momordica charantia])

HSP 1 Score: 793 bits (2049), Expect = 4.38e-287
Identity = 387/468 (82.69%), Postives = 419/468 (89.53%), Query Frame = 0


Query: 1   MGPEDYQFSNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEKEESYKSFGNRWVI 60
           MGPEDYQFSNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEKEESYKSFGNRWVI
Sbjct: 1   MGPEDYQFSNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEKEESYKSFGNRWVI 60

Query: 61  FISILSQKFLLAIANLLEKWEPMRGKFWRVPDHKTYASEVNCRDWKIGT--SIKLPNED- 120
           FISILSQKFLLAIANLLEKWEPMRGKFWRV   +TYASEVNCRDWKIG   +IKL N+D 
Sbjct: 61  FISILSQKFLLAIANLLEKWEPMRGKFWRV---QTYASEVNCRDWKIGKEENIKLLNDDD 120

Query: 121 AFIYYGAVTVMASNLAYQDYSS-EPSVVETVVSSCWKMNLLGCYNFWNDFQNKASTQAFM 180
            F+YYGAVTVMASNL YQD S  E SVV TVV+SCWKM LLGCY+F N F++KA TQA M
Sbjct: 121 TFVYYGAVTVMASNLVYQDCSPPESSVVATVVNSCWKMKLLGCYDFENMFESKARTQATM 180

Query: 181 FQNTATDPNVTVVAFRGTNSIDNIMVDLNFSWCNFDQRIGNIHSGFMEALGLQKGPIGWP 240
           FQNTATDPNVTVVAF+GTN+I + ++DLNFSW N DQRIGNIHSGFMEALGLQKG IGWP
Sbjct: 181 FQNTATDPNVTVVAFKGTNTIYDAILDLNFSWYNVDQRIGNIHSGFMEALGLQKGTIGWP 240

Query: 241 TELPTPDHDFAYYYLRQQLRDIAKSNDKARFVFTGHSLGGALAILFVTILSYHNESDVLN 300
            ELP  DH FAYYYLRQQLRDIAKSNDKA+F+FTGHSLGGALAILFVTILSYHNESDVLN
Sbjct: 241 KELPKSDHQFAYYYLRQQLRDIAKSNDKAKFIFTGHSLGGALAILFVTILSYHNESDVLN 300

Query: 301 KLQAVYTFGQPRVGNSQFAQFMEKTLQQYAFKYYRYVYSMDLAPRIPFDIFDQWYEHFGG 360
           KLQAVYTFGQPRVGNSQF +FME T +QYAFKYYRYVYSMDL PRIPFDIFD+WY+HFGG
Sbjct: 301 KLQAVYTFGQPRVGNSQFVKFMESTSKQYAFKYYRYVYSMDLVPRIPFDIFDEWYKHFGG 360

Query: 361 CVYFNCCYSGKFLEVQPNKNYFSPIWLIPKYLFAWWELIRSLMIPIIKCSSYYFEGFSAL 420
           CVYFNCCYSG+F+EVQPNKNYFSP+WLIPKYLFA WELIRSLM+P+IK S YYFEGF  L
Sbjct: 361 CVYFNCCYSGQFMEVQPNKNYFSPLWLIPKYLFALWELIRSLMMPLIKFSLYYFEGFKTL 420

Query: 421 LVRLFGLLLPGASAHICLNYINLIRWGKIQQPTKDVQKALIPTNYLQA 464
           L+R FGLL+PGASAH+C+NYI L R GK+  P KD+QK LI  + L+A
Sbjct: 421 LLRFFGLLIPGASAHLCVNYIYLARQGKMP-PLKDIQKTLISEDGLEA 464

[Close]

BLAST of MC11g_new0060 vs. NCBI nr
Match: XP_022135562.1 (uncharacterized protein LOC111007483 isoform X1 [Momordica charantia])

HSP 1 Score: 739 bits (1909), Expect = 9.40e-266
Identity = 362/468 (77.35%), Postives = 402/468 (85.90%), Query Frame = 0


Query: 1   MGPEDYQFSNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEKEESYKSFGNRWVI 60
           MG  +Y+FSNDYL LKP+EATLLDLF+F+LPFG ++LRKLVDCP EKEESY SF NRWVI
Sbjct: 1   MGSLEYEFSNDYLKLKPKEATLLDLFIFLLPFGSIKLRKLVDCPPEKEESYTSFTNRWVI 60

Query: 61  FISILSQKFLLAIANLLEKWEPMRGKFWRVPDHKTYASEVNCRDWKIGT--SIKLPNED- 120
           F SILSQK L A+ANLLEKWE    K    P  +TYASEVNCRDWKIG   +IKL N+D 
Sbjct: 61  FFSILSQKSLFALANLLEKWESSTEKLRTAP--QTYASEVNCRDWKIGKEENIKLLNDDD 120

Query: 121 AFIYYGAVTVMASNLAYQDYSS-EPSVVETVVSSCWKMNLLGCYNFWNDFQNKASTQAFM 180
            F+YYGAVTVMASNL YQD S  E SVV TVV+SCWKM LLGCY+F N F++KA TQA M
Sbjct: 121 TFVYYGAVTVMASNLVYQDCSPPESSVVATVVNSCWKMKLLGCYDFENMFESKARTQATM 180

Query: 181 FQNTATDPNVTVVAFRGTNSIDNIMVDLNFSWCNFDQRIGNIHSGFMEALGLQKGPIGWP 240
           FQNTATDPNVTVVAF+GTN+I + ++DLNFSW N DQRIGNIHSGFMEALGLQKG IGWP
Sbjct: 181 FQNTATDPNVTVVAFKGTNTIYDAILDLNFSWYNVDQRIGNIHSGFMEALGLQKGTIGWP 240

Query: 241 TELPTPDHDFAYYYLRQQLRDIAKSNDKARFVFTGHSLGGALAILFVTILSYHNESDVLN 300
            ELP  DH FAYYYLRQQLRDIAKSNDKA+F+FTGHSLGGALAILFVTILSYHNESDVLN
Sbjct: 241 KELPKSDHQFAYYYLRQQLRDIAKSNDKAKFIFTGHSLGGALAILFVTILSYHNESDVLN 300

Query: 301 KLQAVYTFGQPRVGNSQFAQFMEKTLQQYAFKYYRYVYSMDLAPRIPFDIFDQWYEHFGG 360
           KLQAVYTFGQPRVGNSQF +FME T +QYAFKYYRYVYSMDL PRIPFDIFD+WY+HFGG
Sbjct: 301 KLQAVYTFGQPRVGNSQFVKFMESTSKQYAFKYYRYVYSMDLVPRIPFDIFDEWYKHFGG 360

Query: 361 CVYFNCCYSGKFLEVQPNKNYFSPIWLIPKYLFAWWELIRSLMIPIIKCSSYYFEGFSAL 420
           CVYFNCCYSG+F+EVQPNKNYFSP+WLIPKYLFA WELIRSLM+P+IK S YYFEGF  L
Sbjct: 361 CVYFNCCYSGQFMEVQPNKNYFSPLWLIPKYLFALWELIRSLMMPLIKFSLYYFEGFKTL 420

Query: 421 LVRLFGLLLPGASAHICLNYINLIRWGKIQQPTKDVQKALIPTNYLQA 464
           L+R FGLL+PGASAH+C+NYI L R GK+  P KD+QK LI  + L+A
Sbjct: 421 LLRFFGLLIPGASAHLCVNYIYLARQGKMP-PLKDIQKTLISEDGLEA 465

[Close]

BLAST of MC11g_new0060 vs. NCBI nr
Match: XP_022135582.1 (uncharacterized protein LOC111007494 [Momordica charantia])

HSP 1 Score: 719 bits (1855), Expect = 8.62e-260
Identity = 340/340 (100.00%), Postives = 340/340 (100.00%), Query Frame = 0


Query: 128 MASNLAYQDYSSEPSVVETVVSSCWKMNLLGCYNFWNDFQNKASTQAFMFQNTATDPNVT 187
           MASNLAYQDYSSEPSVVETVVSSCWKMNLLGCYNFWNDFQNKASTQAFMFQNTATDPNVT
Sbjct: 1   MASNLAYQDYSSEPSVVETVVSSCWKMNLLGCYNFWNDFQNKASTQAFMFQNTATDPNVT 60

Query: 188 VVAFRGTNSIDNIMVDLNFSWCNFDQRIGNIHSGFMEALGLQKGPIGWPTELPTPDHDFA 247
           VVAFRGTNSIDNIMVDLNFSWCNFDQRIGNIHSGFMEALGLQKGPIGWPTELPTPDHDFA
Sbjct: 61  VVAFRGTNSIDNIMVDLNFSWCNFDQRIGNIHSGFMEALGLQKGPIGWPTELPTPDHDFA 120

Query: 248 YYYLRQQLRDIAKSNDKARFVFTGHSLGGALAILFVTILSYHNESDVLNKLQAVYTFGQP 307
           YYYLRQQLRDIAKSNDKARFVFTGHSLGGALAILFVTILSYHNESDVLNKLQAVYTFGQP
Sbjct: 121 YYYLRQQLRDIAKSNDKARFVFTGHSLGGALAILFVTILSYHNESDVLNKLQAVYTFGQP 180

Query: 308 RVGNSQFAQFMEKTLQQYAFKYYRYVYSMDLAPRIPFDIFDQWYEHFGGCVYFNCCYSGK 367
           RVGNSQFAQFMEKTLQQYAFKYYRYVYSMDLAPRIPFDIFDQWYEHFGGCVYFNCCYSGK
Sbjct: 181 RVGNSQFAQFMEKTLQQYAFKYYRYVYSMDLAPRIPFDIFDQWYEHFGGCVYFNCCYSGK 240

Query: 368 FLEVQPNKNYFSPIWLIPKYLFAWWELIRSLMIPIIKCSSYYFEGFSALLVRLFGLLLPG 427
           FLEVQPNKNYFSPIWLIPKYLFAWWELIRSLMIPIIKCSSYYFEGFSALLVRLFGLLLPG
Sbjct: 241 FLEVQPNKNYFSPIWLIPKYLFAWWELIRSLMIPIIKCSSYYFEGFSALLVRLFGLLLPG 300

Query: 428 ASAHICLNYINLIRWGKIQQPTKDVQKALIPTNYLQAHHQ 467
           ASAHICLNYINLIRWGKIQQPTKDVQKALIPTNYLQAHHQ
Sbjct: 301 ASAHICLNYINLIRWGKIQQPTKDVQKALIPTNYLQAHHQ 340

[Close]

BLAST of MC11g_new0060 vs. NCBI nr
Match: XP_022139747.1 (uncharacterized protein LOC111010582 [Momordica charantia])

HSP 1 Score: 687 bits (1772), Expect = 4.27e-245
Identity = 340/473 (71.88%), Postives = 388/473 (82.03%), Query Frame = 0


Query: 1   MGPEDYQFSNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEKEESYKSFGNRWVI 60
           MG ++ +FS+DYLILKPEEATLL LFLF LPFGY +LR LVDCP  KE SY +FGNRWVI
Sbjct: 1   MGSQENKFSSDYLILKPEEATLLHLFLFTLPFGYRKLRNLVDCPVAKENSYTTFGNRWVI 60

Query: 61  FISILSQKFLLAIANLLEKWEPMRGKFWRVPDHKTYAS-EVNCRDWKI---GTSIKLPNE 120
           FISIL QKFLLAIANL++  + MRGKF     HKT A+ +VNC++WKI     +IKL ++
Sbjct: 61  FISILLQKFLLAIANLIKYCKSMRGKF-----HKTCAAPKVNCKEWKIIGMEKNIKLADD 120

Query: 121 DAFIYYGAVTVMASNLAYQDYSS-EPSVVETVVSSCWKMNLLGCYNFWNDFQNKASTQAF 180
           D F YYGA+TVMASNLAYQDY+S EPSVV+TVV+ CWKMNLLGCY+FWNDFQNKA+TQ F
Sbjct: 121 DTFKYYGALTVMASNLAYQDYNSPEPSVVQTVVNGCWKMNLLGCYDFWNDFQNKATTQVF 180

Query: 181 MFQNTATDPNVTVVAFRGTNSIDNIMVDLNFSWCNFDQRIGNIHSGFMEALGLQKGPIGW 240
           MFQNTATDPNVTVVAFRG++ I + MVDLN SW N +  IG IH GFM+ALGLQKG  GW
Sbjct: 181 MFQNTATDPNVTVVAFRGSSEIYDWMVDLNLSWYNIE-GIGKIHGGFMQALGLQKGT-GW 240

Query: 241 PTELPTPDHDFAYYYLRQQLRDIAKSNDKARFVFTGHSLGGALAILFVTILSYHNESDVL 300
           P EL  PDHDFAYY LRQQLRDI KSND ARF+FTGHSLGGALA+LF T+L++H+++ +L
Sbjct: 241 PKELANPDHDFAYYTLRQQLRDIVKSNDNARFIFTGHSLGGALAVLFATLLAFHDDATLL 300

Query: 301 NKLQAVYTFGQPRVGNSQFAQFMEKTLQQYAFKYYRYVYSMDLAPRIPFDIFDQ-WYEHF 360
            KLQAVYTFGQPRVG+ +FAQFME T+++Y FKYYRYVYS DL PR+PFD  D  WYEHF
Sbjct: 301 EKLQAVYTFGQPRVGDRRFAQFMENTIEKYGFKYYRYVYSSDLVPRVPFDFNDNLWYEHF 360

Query: 361 GGCVYFNCCYSGKFLEVQPNKNYFSPIWLIPKYLFAWWELIRSLMIPIIKCSSYYFEGFS 420
           GGCVYFNC Y+GKFLEVQPNKNYFSPIWLI KY+ AWWELIRSLMIP+IKCSS Y EGFS
Sbjct: 361 GGCVYFNCSYNGKFLEVQPNKNYFSPIWLISKYVSAWWELIRSLMIPVIKCSSDYLEGFS 420

Query: 421 ALLVRLFGLLLPGASAHICLNYINLIRWGKIQQPTKDVQKALIPTNYLQAHHQ 467
            LL+RLFGLL PGASAH+CLNYIN IRWGK        +K LIP     A HQ
Sbjct: 421 NLLLRLFGLLFPGASAHMCLNYINSIRWGKCNH-----EKTLIPA----AEHQ 457

[Close]

BLAST of MC11g_new0060 vs. NCBI nr
Match: XP_022132571.1 (uncharacterized protein LOC111005368 isoform X1 [Momordica charantia])

HSP 1 Score: 618 bits (1593), Expect = 4.45e-218
Identity = 304/459 (66.23%), Postives = 365/459 (79.52%), Query Frame = 0


Query: 1   MGPEDYQFSNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEKEESYKSFGNRWVI 60
           MG ED+QFS+DYLILKPEEATLLDLFLFILPFG+ ++RKLVDCP  KE SY+S  +R +I
Sbjct: 1   MGSEDHQFSDDYLILKPEEATLLDLFLFILPFGFRKIRKLVDCPAAKENSYRSIQSRLII 60

Query: 61  FISILSQKFLLAIANLLEKWEPMRGKFWRVPDHKTYASEVNCRDWKIG--TSIKLPNEDA 120
           F+SIL QK +LAI+NL +K++ MRGK W V  +K YA +VNCRDWKIG   ++KLP ED 
Sbjct: 61  FVSILFQKLVLAISNLAKKFKLMRGKLWAV-SYKPYAPKVNCRDWKIGGEKNVKLP-EDN 120

Query: 121 FIYYGAVTVMASNLAYQDYSSEPSVVETVVSSCWKMNLLGCYNFWNDFQNKASTQAFMFQ 180
           F YY A+TVMAS LAY DYS +P++V+TVV  CWKMNL+  Y F N F  KA T AFMFQ
Sbjct: 121 FKYYSALTVMASQLAYTDYSPQPNIVQTVVEGCWKMNLINTYKFRNGFIEKARTCAFMFQ 180

Query: 181 NTATDPNVTVVAFRGTNSIDNIMVDLNFSWCNFDQRIGNIHSGFMEALGLQKGPIGWPTE 240
            T+TDPNV ++AF+GT+   +  VD+NFSWC+    +G IH GFM+ALGLQ+    WP E
Sbjct: 181 TTSTDPNVIILAFKGTSQCCDCAVDVNFSWCSIP-GVGRIHMGFMQALGLQEA-FHWPDE 240

Query: 241 LPTPDHDFAYYYLRQQLRDIAKSNDKARFVFTGHSLGGALAILFVTILSYHNESDVLNKL 300
           LP P+ DFAYY LRQQLRDIAKSND ARF+FTGHSLGGALA+LFVTIL+YHNESDVL KL
Sbjct: 241 LPKPNSDFAYYTLRQQLRDIAKSNDNARFIFTGHSLGGALAVLFVTILAYHNESDVLEKL 300

Query: 301 QAVYTFGQPRVGNSQFAQFMEKTLQQYAFKYYRYVYSMDLAPRIPFDIFDQW--YEHFGG 360
           QAVYTFGQPRVG+ +FAQFME T ++Y FKYYRYVYS DL  RIPF+  D +  Y+HFGG
Sbjct: 301 QAVYTFGQPRVGDPKFAQFMENTTKKYDFKYYRYVYSFDLVSRIPFNCGDFFCSYKHFGG 360

Query: 361 CVYFNCCYSGKFLEVQPNKNYFSPIWLIPKYLFAWWELIRSLMIPIIKCSSYYFEGFSAL 420
           CVYF+CCY+GKFLE QPN NY+ PI+LIP++L A WE IRSLMIP++KCS  YFEGF  L
Sbjct: 361 CVYFDCCYNGKFLEEQPNNNYYWPIYLIPQFLAALWEFIRSLMIPLVKCSWDYFEGFGNL 420

Query: 421 LVRLFGLLLPGASAHICLNYINLIRWGKIQQPTKDVQKA 455
             R+ GL +P  SAH+ LNYIN  R+GKIQ P+KD++KA
Sbjct: 421 KNRVLGLFIPATSAHMLLNYINSTRYGKIQ-PSKDIKKA 454

[Close]

BLAST of MC11g_new0060 vs. ExPASy TrEMBL
Match: A0A6J1C148 (uncharacterized protein LOC111007483 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007483 PE=4 SV=1)

HSP 1 Score: 793 bits (2049), Expect = 2.12e-287
Identity = 387/468 (82.69%), Postives = 419/468 (89.53%), Query Frame = 0


Query: 1   MGPEDYQFSNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEKEESYKSFGNRWVI 60
           MGPEDYQFSNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEKEESYKSFGNRWVI
Sbjct: 1   MGPEDYQFSNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEKEESYKSFGNRWVI 60

Query: 61  FISILSQKFLLAIANLLEKWEPMRGKFWRVPDHKTYASEVNCRDWKIGT--SIKLPNED- 120
           FISILSQKFLLAIANLLEKWEPMRGKFWRV   +TYASEVNCRDWKIG   +IKL N+D 
Sbjct: 61  FISILSQKFLLAIANLLEKWEPMRGKFWRV---QTYASEVNCRDWKIGKEENIKLLNDDD 120

Query: 121 AFIYYGAVTVMASNLAYQDYSS-EPSVVETVVSSCWKMNLLGCYNFWNDFQNKASTQAFM 180
            F+YYGAVTVMASNL YQD S  E SVV TVV+SCWKM LLGCY+F N F++KA TQA M
Sbjct: 121 TFVYYGAVTVMASNLVYQDCSPPESSVVATVVNSCWKMKLLGCYDFENMFESKARTQATM 180

Query: 181 FQNTATDPNVTVVAFRGTNSIDNIMVDLNFSWCNFDQRIGNIHSGFMEALGLQKGPIGWP 240
           FQNTATDPNVTVVAF+GTN+I + ++DLNFSW N DQRIGNIHSGFMEALGLQKG IGWP
Sbjct: 181 FQNTATDPNVTVVAFKGTNTIYDAILDLNFSWYNVDQRIGNIHSGFMEALGLQKGTIGWP 240

Query: 241 TELPTPDHDFAYYYLRQQLRDIAKSNDKARFVFTGHSLGGALAILFVTILSYHNESDVLN 300
            ELP  DH FAYYYLRQQLRDIAKSNDKA+F+FTGHSLGGALAILFVTILSYHNESDVLN
Sbjct: 241 KELPKSDHQFAYYYLRQQLRDIAKSNDKAKFIFTGHSLGGALAILFVTILSYHNESDVLN 300

Query: 301 KLQAVYTFGQPRVGNSQFAQFMEKTLQQYAFKYYRYVYSMDLAPRIPFDIFDQWYEHFGG 360
           KLQAVYTFGQPRVGNSQF +FME T +QYAFKYYRYVYSMDL PRIPFDIFD+WY+HFGG
Sbjct: 301 KLQAVYTFGQPRVGNSQFVKFMESTSKQYAFKYYRYVYSMDLVPRIPFDIFDEWYKHFGG 360

Query: 361 CVYFNCCYSGKFLEVQPNKNYFSPIWLIPKYLFAWWELIRSLMIPIIKCSSYYFEGFSAL 420
           CVYFNCCYSG+F+EVQPNKNYFSP+WLIPKYLFA WELIRSLM+P+IK S YYFEGF  L
Sbjct: 361 CVYFNCCYSGQFMEVQPNKNYFSPLWLIPKYLFALWELIRSLMMPLIKFSLYYFEGFKTL 420

Query: 421 LVRLFGLLLPGASAHICLNYINLIRWGKIQQPTKDVQKALIPTNYLQA 464
           L+R FGLL+PGASAH+C+NYI L R GK+  P KD+QK LI  + L+A
Sbjct: 421 LLRFFGLLIPGASAHLCVNYIYLARQGKMP-PLKDIQKTLISEDGLEA 464

[Close]

BLAST of MC11g_new0060 vs. ExPASy TrEMBL
Match: A0A6J1C321 (uncharacterized protein LOC111007483 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007483 PE=4 SV=1)

HSP 1 Score: 739 bits (1909), Expect = 4.55e-266
Identity = 362/468 (77.35%), Postives = 402/468 (85.90%), Query Frame = 0


Query: 1   MGPEDYQFSNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEKEESYKSFGNRWVI 60
           MG  +Y+FSNDYL LKP+EATLLDLF+F+LPFG ++LRKLVDCP EKEESY SF NRWVI
Sbjct: 1   MGSLEYEFSNDYLKLKPKEATLLDLFIFLLPFGSIKLRKLVDCPPEKEESYTSFTNRWVI 60

Query: 61  FISILSQKFLLAIANLLEKWEPMRGKFWRVPDHKTYASEVNCRDWKIGT--SIKLPNED- 120
           F SILSQK L A+ANLLEKWE    K    P  +TYASEVNCRDWKIG   +IKL N+D 
Sbjct: 61  FFSILSQKSLFALANLLEKWESSTEKLRTAP--QTYASEVNCRDWKIGKEENIKLLNDDD 120

Query: 121 AFIYYGAVTVMASNLAYQDYSS-EPSVVETVVSSCWKMNLLGCYNFWNDFQNKASTQAFM 180
            F+YYGAVTVMASNL YQD S  E SVV TVV+SCWKM LLGCY+F N F++KA TQA M
Sbjct: 121 TFVYYGAVTVMASNLVYQDCSPPESSVVATVVNSCWKMKLLGCYDFENMFESKARTQATM 180

Query: 181 FQNTATDPNVTVVAFRGTNSIDNIMVDLNFSWCNFDQRIGNIHSGFMEALGLQKGPIGWP 240
           FQNTATDPNVTVVAF+GTN+I + ++DLNFSW N DQRIGNIHSGFMEALGLQKG IGWP
Sbjct: 181 FQNTATDPNVTVVAFKGTNTIYDAILDLNFSWYNVDQRIGNIHSGFMEALGLQKGTIGWP 240

Query: 241 TELPTPDHDFAYYYLRQQLRDIAKSNDKARFVFTGHSLGGALAILFVTILSYHNESDVLN 300
            ELP  DH FAYYYLRQQLRDIAKSNDKA+F+FTGHSLGGALAILFVTILSYHNESDVLN
Sbjct: 241 KELPKSDHQFAYYYLRQQLRDIAKSNDKAKFIFTGHSLGGALAILFVTILSYHNESDVLN 300

Query: 301 KLQAVYTFGQPRVGNSQFAQFMEKTLQQYAFKYYRYVYSMDLAPRIPFDIFDQWYEHFGG 360
           KLQAVYTFGQPRVGNSQF +FME T +QYAFKYYRYVYSMDL PRIPFDIFD+WY+HFGG
Sbjct: 301 KLQAVYTFGQPRVGNSQFVKFMESTSKQYAFKYYRYVYSMDLVPRIPFDIFDEWYKHFGG 360

Query: 361 CVYFNCCYSGKFLEVQPNKNYFSPIWLIPKYLFAWWELIRSLMIPIIKCSSYYFEGFSAL 420
           CVYFNCCYSG+F+EVQPNKNYFSP+WLIPKYLFA WELIRSLM+P+IK S YYFEGF  L
Sbjct: 361 CVYFNCCYSGQFMEVQPNKNYFSPLWLIPKYLFALWELIRSLMMPLIKFSLYYFEGFKTL 420

Query: 421 LVRLFGLLLPGASAHICLNYINLIRWGKIQQPTKDVQKALIPTNYLQA 464
           L+R FGLL+PGASAH+C+NYI L R GK+  P KD+QK LI  + L+A
Sbjct: 421 LLRFFGLLIPGASAHLCVNYIYLARQGKMP-PLKDIQKTLISEDGLEA 465

[Close]

BLAST of MC11g_new0060 vs. ExPASy TrEMBL
Match: A0A6J1C341 (uncharacterized protein LOC111007494 OS=Momordica charantia OX=3673 GN=LOC111007494 PE=4 SV=1)

HSP 1 Score: 719 bits (1855), Expect = 4.17e-260
Identity = 340/340 (100.00%), Postives = 340/340 (100.00%), Query Frame = 0


Query: 128 MASNLAYQDYSSEPSVVETVVSSCWKMNLLGCYNFWNDFQNKASTQAFMFQNTATDPNVT 187
           MASNLAYQDYSSEPSVVETVVSSCWKMNLLGCYNFWNDFQNKASTQAFMFQNTATDPNVT
Sbjct: 1   MASNLAYQDYSSEPSVVETVVSSCWKMNLLGCYNFWNDFQNKASTQAFMFQNTATDPNVT 60

Query: 188 VVAFRGTNSIDNIMVDLNFSWCNFDQRIGNIHSGFMEALGLQKGPIGWPTELPTPDHDFA 247
           VVAFRGTNSIDNIMVDLNFSWCNFDQRIGNIHSGFMEALGLQKGPIGWPTELPTPDHDFA
Sbjct: 61  VVAFRGTNSIDNIMVDLNFSWCNFDQRIGNIHSGFMEALGLQKGPIGWPTELPTPDHDFA 120

Query: 248 YYYLRQQLRDIAKSNDKARFVFTGHSLGGALAILFVTILSYHNESDVLNKLQAVYTFGQP 307
           YYYLRQQLRDIAKSNDKARFVFTGHSLGGALAILFVTILSYHNESDVLNKLQAVYTFGQP
Sbjct: 121 YYYLRQQLRDIAKSNDKARFVFTGHSLGGALAILFVTILSYHNESDVLNKLQAVYTFGQP 180

Query: 308 RVGNSQFAQFMEKTLQQYAFKYYRYVYSMDLAPRIPFDIFDQWYEHFGGCVYFNCCYSGK 367
           RVGNSQFAQFMEKTLQQYAFKYYRYVYSMDLAPRIPFDIFDQWYEHFGGCVYFNCCYSGK
Sbjct: 181 RVGNSQFAQFMEKTLQQYAFKYYRYVYSMDLAPRIPFDIFDQWYEHFGGCVYFNCCYSGK 240

Query: 368 FLEVQPNKNYFSPIWLIPKYLFAWWELIRSLMIPIIKCSSYYFEGFSALLVRLFGLLLPG 427
           FLEVQPNKNYFSPIWLIPKYLFAWWELIRSLMIPIIKCSSYYFEGFSALLVRLFGLLLPG
Sbjct: 241 FLEVQPNKNYFSPIWLIPKYLFAWWELIRSLMIPIIKCSSYYFEGFSALLVRLFGLLLPG 300

Query: 428 ASAHICLNYINLIRWGKIQQPTKDVQKALIPTNYLQAHHQ 467
           ASAHICLNYINLIRWGKIQQPTKDVQKALIPTNYLQAHHQ
Sbjct: 301 ASAHICLNYINLIRWGKIQQPTKDVQKALIPTNYLQAHHQ 340

[Close]

BLAST of MC11g_new0060 vs. ExPASy TrEMBL
Match: A0A6J1CEU2 (uncharacterized protein LOC111010582 OS=Momordica charantia OX=3673 GN=LOC111010582 PE=4 SV=1)

HSP 1 Score: 687 bits (1772), Expect = 2.07e-245
Identity = 340/473 (71.88%), Postives = 388/473 (82.03%), Query Frame = 0


Query: 1   MGPEDYQFSNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEKEESYKSFGNRWVI 60
           MG ++ +FS+DYLILKPEEATLL LFLF LPFGY +LR LVDCP  KE SY +FGNRWVI
Sbjct: 1   MGSQENKFSSDYLILKPEEATLLHLFLFTLPFGYRKLRNLVDCPVAKENSYTTFGNRWVI 60

Query: 61  FISILSQKFLLAIANLLEKWEPMRGKFWRVPDHKTYAS-EVNCRDWKI---GTSIKLPNE 120
           FISIL QKFLLAIANL++  + MRGKF     HKT A+ +VNC++WKI     +IKL ++
Sbjct: 61  FISILLQKFLLAIANLIKYCKSMRGKF-----HKTCAAPKVNCKEWKIIGMEKNIKLADD 120

Query: 121 DAFIYYGAVTVMASNLAYQDYSS-EPSVVETVVSSCWKMNLLGCYNFWNDFQNKASTQAF 180
           D F YYGA+TVMASNLAYQDY+S EPSVV+TVV+ CWKMNLLGCY+FWNDFQNKA+TQ F
Sbjct: 121 DTFKYYGALTVMASNLAYQDYNSPEPSVVQTVVNGCWKMNLLGCYDFWNDFQNKATTQVF 180

Query: 181 MFQNTATDPNVTVVAFRGTNSIDNIMVDLNFSWCNFDQRIGNIHSGFMEALGLQKGPIGW 240
           MFQNTATDPNVTVVAFRG++ I + MVDLN SW N +  IG IH GFM+ALGLQKG  GW
Sbjct: 181 MFQNTATDPNVTVVAFRGSSEIYDWMVDLNLSWYNIE-GIGKIHGGFMQALGLQKGT-GW 240

Query: 241 PTELPTPDHDFAYYYLRQQLRDIAKSNDKARFVFTGHSLGGALAILFVTILSYHNESDVL 300
           P EL  PDHDFAYY LRQQLRDI KSND ARF+FTGHSLGGALA+LF T+L++H+++ +L
Sbjct: 241 PKELANPDHDFAYYTLRQQLRDIVKSNDNARFIFTGHSLGGALAVLFATLLAFHDDATLL 300

Query: 301 NKLQAVYTFGQPRVGNSQFAQFMEKTLQQYAFKYYRYVYSMDLAPRIPFDIFDQ-WYEHF 360
            KLQAVYTFGQPRVG+ +FAQFME T+++Y FKYYRYVYS DL PR+PFD  D  WYEHF
Sbjct: 301 EKLQAVYTFGQPRVGDRRFAQFMENTIEKYGFKYYRYVYSSDLVPRVPFDFNDNLWYEHF 360

Query: 361 GGCVYFNCCYSGKFLEVQPNKNYFSPIWLIPKYLFAWWELIRSLMIPIIKCSSYYFEGFS 420
           GGCVYFNC Y+GKFLEVQPNKNYFSPIWLI KY+ AWWELIRSLMIP+IKCSS Y EGFS
Sbjct: 361 GGCVYFNCSYNGKFLEVQPNKNYFSPIWLISKYVSAWWELIRSLMIPVIKCSSDYLEGFS 420

Query: 421 ALLVRLFGLLLPGASAHICLNYINLIRWGKIQQPTKDVQKALIPTNYLQAHHQ 467
            LL+RLFGLL PGASAH+CLNYIN IRWGK        +K LIP     A HQ
Sbjct: 421 NLLLRLFGLLFPGASAHMCLNYINSIRWGKCNH-----EKTLIPA----AEHQ 457

[Close]

BLAST of MC11g_new0060 vs. ExPASy TrEMBL
Match: A0A6J1BSM4 (uncharacterized protein LOC111005368 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005368 PE=4 SV=1)

HSP 1 Score: 618 bits (1593), Expect = 2.16e-218
Identity = 304/459 (66.23%), Postives = 365/459 (79.52%), Query Frame = 0


Query: 1   MGPEDYQFSNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEKEESYKSFGNRWVI 60
           MG ED+QFS+DYLILKPEEATLLDLFLFILPFG+ ++RKLVDCP  KE SY+S  +R +I
Sbjct: 1   MGSEDHQFSDDYLILKPEEATLLDLFLFILPFGFRKIRKLVDCPAAKENSYRSIQSRLII 60

Query: 61  FISILSQKFLLAIANLLEKWEPMRGKFWRVPDHKTYASEVNCRDWKIG--TSIKLPNEDA 120
           F+SIL QK +LAI+NL +K++ MRGK W V  +K YA +VNCRDWKIG   ++KLP ED 
Sbjct: 61  FVSILFQKLVLAISNLAKKFKLMRGKLWAV-SYKPYAPKVNCRDWKIGGEKNVKLP-EDN 120

Query: 121 FIYYGAVTVMASNLAYQDYSSEPSVVETVVSSCWKMNLLGCYNFWNDFQNKASTQAFMFQ 180
           F YY A+TVMAS LAY DYS +P++V+TVV  CWKMNL+  Y F N F  KA T AFMFQ
Sbjct: 121 FKYYSALTVMASQLAYTDYSPQPNIVQTVVEGCWKMNLINTYKFRNGFIEKARTCAFMFQ 180

Query: 181 NTATDPNVTVVAFRGTNSIDNIMVDLNFSWCNFDQRIGNIHSGFMEALGLQKGPIGWPTE 240
            T+TDPNV ++AF+GT+   +  VD+NFSWC+    +G IH GFM+ALGLQ+    WP E
Sbjct: 181 TTSTDPNVIILAFKGTSQCCDCAVDVNFSWCSIP-GVGRIHMGFMQALGLQEA-FHWPDE 240

Query: 241 LPTPDHDFAYYYLRQQLRDIAKSNDKARFVFTGHSLGGALAILFVTILSYHNESDVLNKL 300
           LP P+ DFAYY LRQQLRDIAKSND ARF+FTGHSLGGALA+LFVTIL+YHNESDVL KL
Sbjct: 241 LPKPNSDFAYYTLRQQLRDIAKSNDNARFIFTGHSLGGALAVLFVTILAYHNESDVLEKL 300

Query: 301 QAVYTFGQPRVGNSQFAQFMEKTLQQYAFKYYRYVYSMDLAPRIPFDIFDQW--YEHFGG 360
           QAVYTFGQPRVG+ +FAQFME T ++Y FKYYRYVYS DL  RIPF+  D +  Y+HFGG
Sbjct: 301 QAVYTFGQPRVGDPKFAQFMENTTKKYDFKYYRYVYSFDLVSRIPFNCGDFFCSYKHFGG 360

Query: 361 CVYFNCCYSGKFLEVQPNKNYFSPIWLIPKYLFAWWELIRSLMIPIIKCSSYYFEGFSAL 420
           CVYF+CCY+GKFLE QPN NY+ PI+LIP++L A WE IRSLMIP++KCS  YFEGF  L
Sbjct: 361 CVYFDCCYNGKFLEEQPNNNYYWPIYLIPQFLAALWEFIRSLMIPLVKCSWDYFEGFGNL 420

Query: 421 LVRLFGLLLPGASAHICLNYINLIRWGKIQQPTKDVQKA 455
             R+ GL +P  SAH+ LNYIN  R+GKIQ P+KD++KA
Sbjct: 421 KNRVLGLFIPATSAHMLLNYINSTRYGKIQ-PSKDIKKA 454

[Close]

BLAST of MC11g_new0060 vs. TAIR 10
Match: AT1G45201.1 (triacylglycerol lipase-like 1 )

HSP 1 Score: 316.6 bits (810), Expect = 3.3e-86
Identity = 178/477 (37.32%), Postives = 270/477 (56.60%), Query Frame = 0


Query: 1   MGPEDYQFSNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEKEESYKSFGNRWVI 60
           M   + +F N Y ++ P +A+ LDL L +        R +   P+  +   +SF +RW++
Sbjct: 1   MSKTNMKFCNSYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWIL 60

Query: 61  FISILSQKFLLA-------IANLLEKW---------------EPMRGKFWRVPDHK--TY 120
            ++I  QK L+        I   L  W                 M GK  + PD    TY
Sbjct: 61  ALAIFLQKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMSGKLVK-PDKSSATY 120

Query: 121 ASEVNCRDWKIGTSIKLPNEDAFIYYGAVTVMASNLAYQDYSSEPSVVETVVSSCWKMNL 180
            S + C D +I    K+ N  +  Y   +++MAS ++Y+   S+P  + +VV + WKM+L
Sbjct: 121 TSFIGCSDRRIELDEKI-NVGSIEYKSMLSIMASKISYE---SKP-YITSVVKNTWKMDL 180

Query: 181 LGCYNFWNDFQNKASTQAFMFQNTATDPNVTVVAFRGTNSID--NIMVDLNFSWCNFDQR 240
           +G Y+F+N FQ    TQAF+F+ ++T+P++ VV+FRGT   +  +   DL+ SW    + 
Sbjct: 181 VGNYDFYNAFQESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEM-KN 240

Query: 241 IGNIHSGFMEALGLQKGPIGWPTELPTPDHDFAYYYLRQQLRDIAKSNDKARFVFTGHSL 300
           +G +H+GF  ALGLQK   GWP E  +  H +AYY +RQ LRD    N   +++ TGHSL
Sbjct: 241 VGKVHAGFSRALGLQKD--GWPKENISLLHQYAYYTIRQMLRDKLGRNKNLKYILTGHSL 300

Query: 301 GGALAILFVTILSYHNESDVLNKLQAVYTFGQPRVGNSQFAQFMEKTLQQYAFKYYRYVY 360
           GGALA LF  IL+ H E ++L+KL+ +YTFGQPRVG+  F +FM+  ++++  +Y R+VY
Sbjct: 301 GGALAALFPAILAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHGIEYERFVY 360

Query: 361 SMDLAPRIPFDIFDQW---YEHFGGCVYFNCCYSGKFLEVQPNKNYFSPIWLIPKYLFAW 420
           + D+ PR+PFD  D++   Y+H+G C  FN  Y GK  E  PN NYF+ +WLIP+ L   
Sbjct: 361 NNDVVPRVPFD--DKYLFSYKHYGPCNSFNSLYKGKVREDAPNANYFNLLWLIPQLLTGL 420

Query: 421 WELIRSLMIPIIKCSSYYFEGFSALLVRLFGLLLPGASAHICLNYINLIRWGKIQQP 449
           WE IRS ++   K    Y E +    VR+ G++ PG S H   +Y+N  R G + +P
Sbjct: 421 WEFIRSFILQFWK-GDEYKENWLMRFVRVVGIVFPGGSNHFPFDYVNSTRLGGLVRP 465

[Close]

BLAST of MC11g_new0060 vs. TAIR 10
Match: AT5G42930.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 312.8 bits (800), Expect = 4.8e-85
Identity = 177/473 (37.42%), Postives = 269/473 (56.87%), Query Frame = 0


Query: 9   SNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEK-EESYKSFGNRWVIFISILSQ 68
           + +Y +L P EAT+ DL + +L    L  RK +   EE+ E+    F  RW+IF+SI+ Q
Sbjct: 4   TKNYFVLDPREATVSDL-IHLLFSSDLGDRKFIHSSEERIEDDLSRFRRRWIIFVSIVIQ 63

Query: 69  K----------------------------FLLAIANLLEKWEPMRGKFWRVPDHKTYASE 128
           K                            FL+ + NL +         W      T+AS 
Sbjct: 64  KLIILFKKPLYFVGFSLACWLNLLSSNGGFLMILPNLFK-----GNIIWPEKASATFASL 123

Query: 129 VNCRDWKIGTSIKLPNEDAFIYYGAVTVMASNLAYQDYSSEPSVVETVVSSCWKMNLLGC 188
               D ++  + K+    +  Y   +++MAS L+Y++     + V +V+ + WKM+LLG 
Sbjct: 124 NGNLDRRVELNPKV-ERGSKRYKAMLSIMASKLSYENI----NFVSSVLHNHWKMDLLGF 183

Query: 189 YNFWNDFQNKASTQAFMFQNTATDPNVTVVAFRGTN--SIDNIMVDLNFSWCNFDQRIGN 248
           Y+ WN +Q + ST+  + ++T+TDPN+ +V+FRGT+    D+   DL+ SW    + +G 
Sbjct: 184 YSCWNGYQKQRSTEVIVIKDTSTDPNLIIVSFRGTDPFDADDWCTDLDLSWYEV-KNVGK 243

Query: 249 IHSGFMEALGLQKGPIGWPTELPTPDHD-----FAYYYLRQQLRDIAKSNDKARFVFTGH 308
           IH GFM+ALGLQK   GWP E+   +       +AYY +R+ L++I   N  ++F+ TGH
Sbjct: 244 IHGGFMKALGLQKE--GWPKEVNLDETQNATTLYAYYTVRRHLKEILDQNPTSKFILTGH 303

Query: 309 SLGGALAILFVTILSYHNESDVLNKLQAVYTFGQPRVGNSQFAQFMEKTLQQYAFKYYRY 368
           SLGGALAILF  +L  H+E  +L +L+ VYTFGQPRVG+ +F  FM+ +L+++  KY RY
Sbjct: 304 SLGGALAILFTAVLVMHDEEQMLERLEGVYTFGQPRVGDEEFGNFMKDSLKKFDVKYERY 363

Query: 369 VYSMDLAPRIPFDIFDQWYEHFGGCVYFNCCYSGKFLEVQPNKNYFSPIWLIPKYLFAWW 428
           VY  D+ PR+PFD     ++HFG C+Y++  Y GK  E +PNKNYF+ +W++PK + A W
Sbjct: 364 VYCNDMVPRLPFDDKTLMFKHFGACLYYDSFYKGKVEEEEPNKNYFNLVWVLPKIMNALW 423

Query: 429 ELIRSLMIPIIKCSSYYFEGFSALLVRLFGLLLPGASAHICLNYINLIRWGKI 446
           ELIRS ++P  K    + EG+     R+  LL+PG  AH    YIN+   G +
Sbjct: 424 ELIRSFVMPYWK-GGEFREGWFLRCFRVVALLIPGLPAHFPNEYINVTLLGDL 461

[Close]

BLAST of MC11g_new0060 vs. TAIR 10
Match: AT1G56630.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 302.8 bits (774), Expect = 4.9e-82
Identity = 184/480 (38.33%), Postives = 264/480 (55.00%), Query Frame = 0


Query: 11  DYLILKPEEATLLDLFLFILPFGYLRLRKLVDCP-EEKEESYKSFGNRWVIFISILSQKF 70
           +Y +L P EAT+ DL + +L +  L  RK VD   E  E     F  RW+IF+SI+ QK 
Sbjct: 6   NYFVLDPREATVTDL-MRLLFYSDLENRKFVDTSVENLENRLCEFRGRWIIFVSIVVQKL 65

Query: 71  LLAIAN-------LLEKW---------------EPMRGKF-WRVPDHKTYASEVNCRDWK 130
           L+ +          L  W                 ++G+F W      T+AS     D K
Sbjct: 66  LIILRKPLSFLGFALGFWLNLPSSNGGFFKIFLNLVKGRFIWPEKTSATFASINGNLDQK 125

Query: 131 I----GTSIKLPNEDAFIYYGAVTVMASNLAYQDYSSEPSVVETVVSSCWKMNLLGCYNF 190
           +    G SIK+ +E    Y   +++MAS LAY++       + +V+   W+M+LLG Y+ 
Sbjct: 126 VELGLGRSIKIGDER---YKPLLSIMASKLAYEN----EHFIRSVLQDHWQMDLLGFYSC 185

Query: 191 WNDFQNKASTQAFMFQNTATDPNVTVVAFRGTN--SIDNIMVDLNFSWCNFDQRIGNIHS 250
            NDF    ST+  + ++T  +PN+ VV+FRGT+  + D+   DL+ SW N    +G IH 
Sbjct: 186 PNDFDQTRSTEVIVIRDTKDNPNLIVVSFRGTDPFNADDWCTDLDLSWHNV-MNVGKIHG 245

Query: 251 GFMEALGLQKGPIGWPTELPTPD-----HDFAYYYLRQQLRDIAKSNDKARFVFTGHSLG 310
           GFM+ALGL K   GW  E+ +          AYY + +QL+++ + N  ++F+ +GHSLG
Sbjct: 246 GFMKALGLPKE--GWYEEINSDQTQNKTSQLAYYTILRQLKEVFEQNPTSKFILSGHSLG 305

Query: 311 GALAILFVTILSYHNESDVLNKLQAVYTFGQPRVGNSQFAQFMEKTLQQYAFKYYRYVYS 370
           GALAILF  +L  H+E ++L +L+ VYTFGQPRVG+  F  +M+  L+++  KY RYVY 
Sbjct: 306 GALAILFTAVLIMHDEKEMLERLEGVYTFGQPRVGDEDFGIYMKDKLKEFDVKYKRYVYC 365

Query: 371 MDLAPRIPFDIFDQWYEHFGGCVYFNCCYSGKFLEVQPNKNYFSPIWLIPKYLFAWWELI 430
            D+ PR+PFD     ++HFGGC+Y +  Y GK  E +PNKNYF+  W+IPK + A WELI
Sbjct: 366 NDMVPRLPFDDKTLMFKHFGGCLYCDSFYKGKVEEEEPNKNYFNIFWVIPKIINAVWELI 425

Query: 431 RSLMIPIIKC--SSYYFEGFSALLVRLFGLLLPGASAHICLNYINLIRWGKIQQPTKDVQ 454
           RS    II C     Y EG+     RL  LL+PG  AH    Y+N+   G       D Q
Sbjct: 426 RSF---IISCWQGREYREGWLLTCFRLVALLIPGLPAHFPNEYVNVALLGNFPPQVPDSQ 471

[Close]

BLAST of MC11g_new0060 vs. TAIR 10
Match: AT5G67050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 282.3 bits (721), Expect = 6.9e-76
Identity = 172/471 (36.52%), Postives = 256/471 (54.35%), Query Frame = 0


Query: 1   MGPEDYQFSNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEKEESYKSFGNRWVI 60
           M  +D      YLIL+PEE    +L + +L  G +   + VD  E +E S++   +RW+I
Sbjct: 2   MNSDDDDEPRGYLILRPEELRPWEL-VRLLFSGDIEKPRSVDSSETEEHSFR---HRWLI 61

Query: 61  FISILSQKFLLAIANLLE-------------KWEPMRGKFWR----VPDH--KTYASEVN 120
           F+S++  K L   + LL                    G F R    +P    + Y S + 
Sbjct: 62  FVSLVLLKLLRFFSKLLALVGSALEFSLNFLSNNSFSGLFLRGEVVMPQRTSENYQSFIG 121

Query: 121 CRDWKIGTSIKLPNEDAFIYYGAVTVMASNLAYQDYSSEPSVVETVVSSCWKMNLLGCYN 180
             D ++   + L  ED   YY A+++MAS +AY++ +     ++ VV + W M  LG  +
Sbjct: 122 HLDTRVSLDMTLNREDGEKYYAALSIMASKIAYENAAR----IKHVVENHWNMKYLGLVD 181

Query: 181 FWNDFQNKASTQAFMFQNTAT------DPNVTVVAFRGTN--SIDNIMVDLNFSWCNFDQ 240
           +WN++Q K +TQAF+     T           VVAFRGT   + ++   D + +W     
Sbjct: 182 YWNEYQEKETTQAFIMSTDETTTRSNGQETTVVVAFRGTELFNSEDWCSDFDITWFEL-P 241

Query: 241 RIGNIHSGFMEALGLQKGPIGWPTE-LPTPDH--DFAYYYLRQQLRDIAKSNDKARFVFT 300
            IGNIH GFM+ALGLQ     WP E L  PD     AYY +R  L+ +   N   +FV T
Sbjct: 242 NIGNIHGGFMKALGLQNN-CSWPKEPLSNPDRKSPLAYYSIRDSLKTLIAQNKNTKFVLT 301

Query: 301 GHSLGGALAILFVTILSYHNESDVLNKLQAVYTFGQPRVGNSQFAQFMEKTLQQYAFKYY 360
           GHSLGGALAILF  +L  H+E+++L ++Q VYT+GQPRVG+S+F +FMEK L++Y  KYY
Sbjct: 302 GHSLGGALAILFTAVLVIHHETELLERIQGVYTYGQPRVGDSKFGEFMEKKLEKYNIKYY 361

Query: 361 RYVYSMDLAPRIPFDIFDQWYEHFGGCVYFNCCYSGKFLEVQPNKNYFSPIWLIPKYLFA 420
           R+VY+ D+ PR+P+D  D  ++HFG C+Y++  Y  K +  Q ++N+F    +I     A
Sbjct: 362 RFVYNNDIVPRLPYDDKDLMFKHFGTCIYYDQNYQAKVMREQSDENFFLLRGIIKMMWSA 421

Query: 421 WWELIRSLMIPIIKCSSYYFEGFSALLVRLFGLLLPGASAHICLNYINLIR 442
             E IRS  I + +  S Y EG+     R  G+++PG S H   +Y+N  R
Sbjct: 422 ILEFIRSFTI-VAEKGSEYSEGWLLKGGRALGIIVPGVSNHTPQDYVNATR 461

[Close]

BLAST of MC11g_new0060 vs. TAIR 10
Match: AT1G45201.2 (triacylglycerol lipase-like 1 )

HSP 1 Score: 260.0 bits (663), Expect = 3.7e-69
Identity = 147/396 (37.12%), Postives = 226/396 (57.07%), Query Frame = 0


Query: 1   MGPEDYQFSNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEKEESYKSFGNRWVI 60
           M   + +F N Y ++ P +A+ LDL L +        R +   P+  +   +SF +RW++
Sbjct: 1   MSKTNMKFCNSYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWIL 60

Query: 61  FISILSQKFLLA-------IANLLEKW---------------EPMRGKFWRVPDHK--TY 120
            ++I  QK L+        I   L  W                 M GK  + PD    TY
Sbjct: 61  ALAIFLQKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMSGKLVK-PDKSSATY 120

Query: 121 ASEVNCRDWKIGTSIKLPNEDAFIYYGAVTVMASNLAYQDYSSEPSVVETVVSSCWKMNL 180
            S + C D +I    K+ N  +  Y   +++MAS ++Y+   S+P  + +VV + WKM+L
Sbjct: 121 TSFIGCSDRRIELDEKI-NVGSIEYKSMLSIMASKISYE---SKP-YITSVVKNTWKMDL 180

Query: 181 LGCYNFWNDFQNKASTQAFMFQNTATDPNVTVVAFRGTNSID--NIMVDLNFSWCNFDQR 240
           +G Y+F+N FQ    TQAF+F+ ++T+P++ VV+FRGT   +  +   DL+ SW    + 
Sbjct: 181 VGNYDFYNAFQESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEM-KN 240

Query: 241 IGNIHSGFMEALGLQKGPIGWPTELPTPDHDFAYYYLRQQLRDIAKSNDKARFVFTGHSL 300
           +G +H+GF  ALGLQK   GWP E  +  H +AYY +RQ LRD    N   +++ TGHSL
Sbjct: 241 VGKVHAGFSRALGLQKD--GWPKENISLLHQYAYYTIRQMLRDKLGRNKNLKYILTGHSL 300

Query: 301 GGALAILFVTILSYHNESDVLNKLQAVYTFGQPRVGNSQFAQFMEKTLQQYAFKYYRYVY 360
           GGALA LF  IL+ H E ++L+KL+ +YTFGQPRVG+  F +FM+  ++++  +Y R+VY
Sbjct: 301 GGALAALFPAILAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHGIEYERFVY 360

Query: 361 SMDLAPRIPFDIFDQW---YEHFGGCVYFNCCYSGK 368
           + D+ PR+PFD  D++   Y+H+G C  FN  Y GK
Sbjct: 361 NNDVVPRVPFD--DKYLFSYKHYGPCNSFNSLYKGK 385

The following BLAST results are available for this feature:

BLAST of MC11g_new0060 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A1S3ZP85	6.1e-61	37.04	Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1	[more]
F4JFU8	1.5e-59	32.09	Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1	[more]
Q5VKJ7	8.2e-58	39.24	Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1	[more]
O59952	4.4e-11	34.62	Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1	[more]
B8NIB8	5.4e-09	31.07	Probable feruloyl esterase A OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1...	[more]

BLAST of MC11g_new0060 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135571.1	4.38e-287	82.69	uncharacterized protein LOC111007483 isoform X2 [Momordica charantia]	[more]
XP_022135562.1	9.40e-266	77.35	uncharacterized protein LOC111007483 isoform X1 [Momordica charantia]	[more]
XP_022135582.1	8.62e-260	100.00	uncharacterized protein LOC111007494 [Momordica charantia]	[more]
XP_022139747.1	4.27e-245	71.88	uncharacterized protein LOC111010582 [Momordica charantia]	[more]
XP_022132571.1	4.45e-218	66.23	uncharacterized protein LOC111005368 isoform X1 [Momordica charantia]	[more]

BLAST of MC11g_new0060 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C148	2.12e-287	82.69	uncharacterized protein LOC111007483 isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1C321	4.55e-266	77.35	uncharacterized protein LOC111007483 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1C341	4.17e-260	100.00	uncharacterized protein LOC111007494 OS=Momordica charantia OX=3673 GN=LOC111007...	[more]
A0A6J1CEU2	2.07e-245	71.88	uncharacterized protein LOC111010582 OS=Momordica charantia OX=3673 GN=LOC111010...	[more]
A0A6J1BSM4	2.16e-218	66.23	uncharacterized protein LOC111005368 isoform X1 OS=Momordica charantia OX=3673 G...	[more]

BLAST of MC11g_new0060 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G45201.1	3.3e-86	37.32	triacylglycerol lipase-like 1	[more]
AT5G42930.1	4.8e-85	37.42	alpha/beta-Hydrolases superfamily protein	[more]
AT1G56630.1	4.9e-82	38.33	alpha/beta-Hydrolases superfamily protein	[more]
AT5G67050.1	6.9e-76	36.52	alpha/beta-Hydrolases superfamily protein	[more]
AT1G45201.2	3.7e-69	37.12	triacylglycerol lipase-like 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002921	Fungal lipase-like domain	PFAM	PF01764	Lipase_3	coord: 188..345 e-value: 4.6E-38 score: 130.3
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 122..380 e-value: 2.9E-47 score: 163.1
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 129..374
None	No IPR available	PANTHER	PTHR46086:SF4	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 7..450
None	No IPR available	CDD	cd00519	Lipase_3	coord: 127..361 e-value: 3.49434E-38 score: 136.837
IPR044819	Triacylglycerol lipase OBL1-like	PANTHER	PTHR46086	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 7..450

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC11g_new0060.1	MC11g_new0060.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004806	triglyceride lipase activity