MC11g_new0047 (gene) Bitter gourd (Dali-11) v1

BLAST of MC11g_new0047 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 4.7e-140
Identity = 244/510 (47.84%), Postives = 336/510 (65.88%), Query Frame = 0

Query: 57  NPTSVATLVGIFVFCYYYLRRKWQERKQAPSPPGAWPIIGHLHLLHNAKLPHHTLGAMAD 116
           N  ++A+++ +   C  +L RK    K AP   GAWPI+GHL LL+ ++ PH  LGA+AD
Sbjct: 11  NTIAIASILSLIFLC-LFLYRKNSRGKDAPVVSGAWPILGHLSLLNGSQTPHKVLGALAD 70

Query: 117 KYGPIFRLQLGCRSALVVSNWEMAKESMCINDAAAVSRPGISGTKHFSYDYAAFGLAPYS 176
           KYGP+F ++LG + ALV+SNWEM+KE    ND A  SRP +   +  SY+ A  GLAPY 
Sbjct: 71  KYGPLFTIKLGMKPALVLSNWEMSKELFTTNDLAVSSRPKLVAVEVMSYNQAFVGLAPYG 130

Query: 177 PYWREMRKITHLELLSNSRVDQVKNLMFAQIDMSLAEL--------HAGSAQIPVEMKRW 236
           PYWRE+RKI   E LSN R++Q  ++  +++  S+ EL           S    V++K+W
Sbjct: 131 PYWRELRKIVTFEFLSNRRIEQRNHIRVSEVRTSIKELFDIWSNGNKNESRYTLVDIKQW 190

Query: 237 FGDVIVNMLLKIIIGKRCVGPNAEGGEKGAKDLQMAIRDSFHLMGQGLLTDYIPLIGRLG 296
              +  NM++++++GKR  G     G+  A+     IR+  +LMG   + D +P +  L 
Sbjct: 191 LAYLTFNMVVRMVVGKRYFGVMHVEGKDKAQRFMKNIREFMNLMGTFTVADGVPCLRWLD 250

Query: 297 FDGQVKVMESIAKRFDVIMSEWLEEHKRKRDYGLGQNQDGDFMDSLISLLDGKGIQGYYD 356
             G  K M++ AK  D ++SEWLEEH++K+  G     D DFMD +IS L+G  I G +D
Sbjct: 251 LGGHEKAMKANAKEVDKLLSEWLEEHRQKKLLGENVESDRDFMDVMISALNGAQI-GAFD 310

Query: 357 ADTIIKATTLNMIAGGTESTTVTLTWTMTLLLNNPHTLEMAQQELDAVVGRDRRVNESDI 416
           ADTI KAT+L +I GGT+ST VTLTW ++LLL NP  L  A++E+D  +G+D  + ESDI
Sbjct: 311 ADTICKATSLELILGGTDSTAVTLTWALSLLLRNPLALGKAKEEIDMQIGKDEYIRESDI 370

Query: 417 SKLHYLQAVVKEAMRMYPAGPLLGPREFSSDCVVAGYFVPRGTQLIPNIWKIQTDPRVWP 476
           SKL YLQA+VKE +R+YP  P   PREF+ +C++ GY + +GT+LI N+WKI  DP VW 
Sbjct: 371 SKLVYLQAIVKETLRLYPPAPFSSPREFTENCILGGYHIKKGTRLIHNLWKIHRDPSVWS 430

Query: 477 NPFEFKPERFLTTHKDVDAKGNHFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQSFDVK 536
           +P EFKPERFLTTHKDVD +G++FEL+PFGSGRR C G++ GL MVHFTLA  L SFD+ 
Sbjct: 431 DPLEFKPERFLTTHKDVDLRGHNFELLPFGSGRRVCAGMSLGLNMVHFTLANLLHSFDIL 490

Query: 537 SSSADLIDMSENFGMANEKVVPLNVLVTSR 559
           + SA+ +DM+E FG  N K  PL +LV  R
Sbjct: 491 NPSAEPVDMTEFFGFTNTKATPLEILVKPR 518

BLAST of MC11g_new0047 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 1.1e-136
Identity = 247/509 (48.53%), Postives = 339/509 (66.60%), Query Frame = 0

Query: 57  NPTSVATLVGIFVFC---YYYLRRKWQERKQAPSPPGAWPIIGHLHLLHNAKLPHHTLGA 116
           N T++  +  IF+ C   Y  L++     K+AP+  GAWPI GHL LL  +K PH  LGA
Sbjct: 9   NTTTIGVVSLIFLLCLFLYGPLKKVHGSSKEAPTVGGAWPIFGHLPLLIGSKSPHKALGA 68

Query: 117 MADKYGPIFRLQLGCRSALVVSNWEMAKESMCINDAAAVSRPGISGTKHFSYDYAAFGLA 176
           +A+K+GP+F ++LG + ALVVS+WEMA+E    ND A  +RP +   +   Y+ A   +A
Sbjct: 69  LAEKHGPLFTIKLGAKKALVVSDWEMARECFTTNDVAVSARPKLLVAELMCYNNAMLLVA 128

Query: 177 PYSPYWREMRKITHLELLSNSRVDQVKNLMFAQIDMSLAELH-------AGSAQIPVEMK 236
           PY PYWRE+RKI   E+LS+SRV+Q++++  +++  S+ EL+         S    VE+K
Sbjct: 129 PYGPYWRELRKIIVTEILSSSRVEQLQDVRVSEVQNSIVELYDVWRSQKNESDYASVELK 188

Query: 237 RWFGDVIVNMLLKIIIGKRCVGPNAEGGEKGAKDLQMAIRDSFHLMGQGLLTDYIPLIGR 296
           +WF   I NM+L++++GKR +  +A   ++ A+    A+ +   L G   + D IP +  
Sbjct: 189 QWFAQPIFNMVLRMVVGKRFL--SATATDEKAEKCVKAVDEFMRLAGVFTVGDAIPYLRW 248

Query: 297 LGFDGQVKVMESIAKRFDVIMSEWLEEHKRKRDYGLGQNQDGDFMDSLISLLDGKGIQGY 356
           L F G  K M+  AK  DV++SEWLEEH++KR  G G +   DFM+ ++S LDGK I G 
Sbjct: 249 LDFGGYEKAMKETAKELDVMISEWLEEHRQKRALGEGVDGAQDFMNVMLSSLDGKTIDG- 308

Query: 357 YDADTIIKATTLNMIAGGTESTTVTLTWTMTLLLNNPHTLEMAQQELDAVVGRDRRVNES 416
            DADT+IK+T L +I  GTE++  T+ W M L+L NP  LE  + ELD  VG+DR + ES
Sbjct: 309 IDADTLIKSTVLTIIQAGTEASISTIIWAMCLILKNPLILE-NKAELDIQVGKDRCICES 368

Query: 417 DISKLHYLQAVVKEAMRMYPAGPLLGPREFSSDCVVAGYFVPRGTQLIPNIWKIQTDPRV 476
           DIS L YLQAVVKE +R+Y  GPL  PREF+ DC + GY V +GT+LI NIWKI TDP V
Sbjct: 369 DISNLVYLQAVVKETLRLYAPGPLSSPREFAEDCTLGGYHVKKGTRLITNIWKIHTDPNV 428

Query: 477 WPNPFEFKPERFLTTHKDVDAKGNHFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQSFD 536
           W +PFEFKP+RFLTTHKD+D KG+HF+L+PFGSGRR CPG++FGLQ VH  LA FL SF+
Sbjct: 429 WSDPFEFKPDRFLTTHKDIDVKGHHFQLLPFGSGRRVCPGISFGLQTVHLALASFLHSFE 488

Query: 537 VKSSSADLIDMSENFGMANEKVVPLNVLV 556
           + + S + +DM+E FG+ N K  PL VLV
Sbjct: 489 ILNPSTEPLDMTEAFGVTNTKATPLEVLV 513

BLAST of MC11g_new0047 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 478.8 bits (1231), Expect = 8.6e-134
Identity = 236/529 (44.61%), Postives = 335/529 (63.33%), Query Frame = 0

Query: 57  NPTSVATLVGIFVFCYYYLRRKWQERKQAPSPPGAWPIIGHLHLLHNAKLPHHTLGAMAD 116
           N T++A +  I +  + +   K    K+ P   G+WP++GHL L+ N + PH TLGA+ D
Sbjct: 7   NTTTIAFISLISLLFFLFRFSKVSHTKEPPIISGSWPLLGHLPLMRNTQTPHKTLGALVD 66

Query: 117 KYGPIFRLQLGCRSALVVSNWEMAKESMCINDAAAVSRPGISGTKHFSYDYAAFGLAPYS 176
           KYGPIF ++LG  +ALV+SNWE+AKE    ND    SRP     +  SY+ A  G APY 
Sbjct: 67  KYGPIFTIKLGATNALVLSNWELAKECFTKNDIVVSSRPKPVAVELMSYNQAFIGWAPYG 126

Query: 177 PYWREMRKITHLELLSNSRVDQVKNLMFAQIDMSLAEL-HAGSAQI-------------- 236
            YWR++RKI  LE+LSN R++ + ++  +++  S+ EL +  S QI              
Sbjct: 127 AYWRQLRKIVTLEILSNRRIELLSHIRVSEVQTSIKELVNVWSNQISSQYGLLDDTKSSS 186

Query: 237 -----------PVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKGAKDLQMAIRDSFH 296
                       VE+K+WF  + +NM+L++++GKRC G      ++ AK     IRD   
Sbjct: 187 TNDEPSTTDYVSVELKKWFAQLTLNMVLRMVVGKRCFGDVDVENKEEAKRFLENIRDFMR 246

Query: 297 LMGQGLLTDYIPLIGRLGFDGQVKVMESIAKRFDVIMSEWLEEHKRKRDYGLGQNQ---- 356
           L+G   + D +P +  L   G  K M+  AK+FDV+++EWLEEH+ K+  GLG       
Sbjct: 247 LIGTFTVGDGVPFLKWLDLGGHEKEMKKCAKKFDVMLNEWLEEHREKK--GLGSEDKVVG 306

Query: 357 DGDFMDSLISLLDGKGIQGYYDADTIIKATTLNMIAGGTESTTVTLTWTMTLLLNNPHTL 416
           + DFMD+++ +L  K I+G +D DTIIKATTL +I GG+++T  TLTW M LLL +PH L
Sbjct: 307 ERDFMDAMLLVLKDKPIEG-FDVDTIIKATTLELILGGSDTTAGTLTWAMCLLLKHPHVL 366

Query: 417 EMAQQELDAVVGRDRRVNESDISKLHYLQAVVKEAMRMYPAGPLLGPREFSSDCVVAGYF 476
           E  ++EL+  +G++R VNESDI+KL YL A++KE +R+YP  P   PREF+ DC + GY 
Sbjct: 367 EKLKEELNTYIGKERCVNESDINKLVYLHAIIKETLRLYPPAPFSSPREFTEDCTIGGYH 426

Query: 477 VPRGTQLIPNIWKIQTDPRVWPNPFEFKPERFLTTHKDVDAKGNHFELIPFGSGRRGCPG 536
           + +GT+L+PN+WKI  DP VWP+P EFKPERFL+THKDVD +G +FEL+PFGSGRR C G
Sbjct: 427 IKKGTRLMPNLWKIHRDPSVWPDPLEFKPERFLSTHKDVDVRGQNFELLPFGSGRRMCAG 486

Query: 537 VAFGLQMVHFTLAGFLQSFDVKSSSADLIDMSENFGMANEKVVPLNVLV 556
           ++ GL MVH+ LA FL SF++ + S + ID++E       K  PL VLV
Sbjct: 487 MSLGLHMVHYILANFLHSFEILNPSPESIDVTEVLEFVTTKATPLEVLV 532

BLAST of MC11g_new0047 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 3.7e-129
Identity = 234/512 (45.70%), Postives = 329/512 (64.26%), Query Frame = 0

Query: 67  IFVFCYYYLRRKWQERKQAPSPPGAWPIIGHLHLL-HNAKLPHHTLGAMADKYGPIFRLQ 126
           +FVF   + + K  +  +AP+P GAWPIIGHLHLL    +L + TLG MAD YGP   LQ
Sbjct: 14  VFVFIALFKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSLQ 73

Query: 127 LGCRSALVVSNWEMAKESMCINDAAAVSRPGISGTKHFSYDYAAFGLAPYSPYWREMRKI 186
           LG   A VVS++E+AK+   +ND A  SRP  +  KH  Y++A FG APYS +WREMRKI
Sbjct: 74  LGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWREMRKI 133

Query: 187 THLELLSNSRVDQVKNLMFAQIDMSLAELHA------GSAQIPVEMKRWFGDVIVNMLLK 246
             +ELLSN R+  +K++  ++I M + +L++      G+  + V++K W  D+ +NM+++
Sbjct: 134 ATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKPVMVDLKSWLEDMTLNMIVR 193

Query: 247 IIIGKRCVGPNAEGGEKGAKDLQ------MAIRDSFHLMGQGLLTDYIPLIGRLGFDGQV 306
           ++ GKR  G    GG   ++D +       AI   FHL+G   ++D  P +      G  
Sbjct: 194 MVAGKRYFG---GGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDLQGHE 253

Query: 307 KVMESIAKRFDVIMSEWLEEHKRKRDYGLGQNQDGDFMDSLISLLD-GKGIQGYYDADTI 366
           K M+      DVI+  W+E H+++R +   +  D DF+D ++SL + GK     YDA+T 
Sbjct: 254 KEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMSLAEQGKLSHLQYDANTS 313

Query: 367 IKATTLNMIAGGTESTTVTLTWTMTLLLNNPHTLEMAQQELDAVVGRDRRVNESDISKLH 426
           IK+T L +I GG++++  TLTW ++LLLNN   L+ AQ E+D  VGRDR V +SDI  L 
Sbjct: 314 IKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVEDSDIENLV 373

Query: 427 YLQAVVKEAMRMYPAGPLLGPREFSSDCVVAGYFVPRGTQLIPNIWKIQTDPRVWPNPFE 486
           YLQA++KE +R+YPAGPLLGPRE   DC VAGY+VP GT+LI N+WKIQ DP+V+  P E
Sbjct: 374 YLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPKVYMEPNE 433

Query: 487 FKPERFLTTH-KDVDAKGNHFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQSFDVKSSS 546
           F+PERF+T   K+ D +G +FEL+PFGSGRR CPG +  +Q++H  LA FL SFDVK+  
Sbjct: 434 FRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLHSFDVKTVM 493

Query: 547 ADLIDMSENFGMANEKVVPLNVLVTSRLPSHL 564
              +DMSEN G+   K  PL VL++ R+   L
Sbjct: 494 DMPVDMSENPGLTIPKATPLEVLISPRIKEEL 522

BLAST of MC11g_new0047 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 2.4e-128
Identity = 232/507 (45.76%), Postives = 321/507 (63.31%), Query Frame = 0

Query: 64  LVGIFV----FCYYYLRRKWQERKQAPSPPGAWPIIGHLHLLHNAKLPHHTLGAMADKYG 123
           L+G FV    F Y+  ++   + K+ P P GAWPIIGHLHLL    LPH  L + ADK G
Sbjct: 11  LLGFFVLLPCFFYFVWKKPNNKIKEPPQPAGAWPIIGHLHLLARGDLPHKILSSFADKNG 70

Query: 124 PIFRLQLGCRSALVVSNWEMAKESMCINDAAAVSRPGISGTKHFSYDYAAFGLAPYSPYW 183
           P+F++QLG   ALVV+N E+AKE    ND   ++RP     K   Y+Y   G+APY PYW
Sbjct: 71  PVFKIQLGVHQALVVNNSEIAKECFTTNDRFFLNRPSGVAAKIMGYNYVMLGVAPYGPYW 130

Query: 184 REMRKITHLELLSNSRVDQVKNLMFAQIDMSLAELHA--GSAQIP---VEMKRWFGDVIV 243
           R+MRKI  LE LSN R+  +K++  ++I +S  EL+    +  I    V+MK+W  D+ +
Sbjct: 131 RDMRKIIMLEFLSNRRLQSLKHVWHSEISISSKELYKLWETQNIDFCLVDMKQWLADLTL 190

Query: 244 NMLLKIIIGKRCVGPNAEGG--EKGAKDLQMAIRDSFHLMGQGLLTDYIPLIGRLGFDGQ 303
           NM +K+++GKR  G  +     E  + +    +R+ F LMG  +L+DY+P +  L   G 
Sbjct: 191 NMSVKMVVGKRFFGSASASACEETESSNCPKTLRNMFRLMGSFVLSDYLPYLRWLDLGGH 250

Query: 304 VKVMESIAKRFDVIMSEWLEEHKRKRDYGLGQNQDGDFMDSLISLLDGKGIQGYYDADTI 363
            K M+   K  D++   WL+EHKRKR  G  ++ D DFMD ++S+L+   +    D DTI
Sbjct: 251 EKEMKRTVKELDILFKGWLDEHKRKRLSGGKEDDDQDFMDVMLSILEESKLGN--DVDTI 310

Query: 364 IKATTLNMIAGGTESTTVTLTWTMTLLLNNPHTLEMAQQELDAVVGRDRRVNESDISKLH 423
            K   L +I GG ++T  TLTW ++LLLNNP+ L+ AQ ELD  VGRDR V+ESD+ KL 
Sbjct: 311 NKTACLAIILGGADTTWATLTWALSLLLNNPNALKKAQDELDLHVGRDRNVDESDLVKLT 370

Query: 424 YLQAVVKEAMRMYPAGPLLGPREFSSDCVVAGYFVPRGTQLIPNIWKIQTDPRVWPNPFE 483
           Y+ A++KE +R+YP GPLLGPR  + DC +AGY V  GT+LI N WKIQ DP VW  P E
Sbjct: 371 YIDAIIKETLRLYPPGPLLGPRVVTEDCTIAGYHVRAGTRLIVNAWKIQRDPLVWSQPHE 430

Query: 484 FKPERFLTTHKDVDAKGNHFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQSFDVKSSSA 543
           ++PERFL   +DVD KG HFELIPFGSGRR CP ++  LQ++  TLA  L  F++++ + 
Sbjct: 431 YQPERFL--ERDVDMKGQHFELIPFGSGRRACPAISLALQVLPLTLAHILHGFELRTPNQ 490

Query: 544 DLIDMSENFGMANEKVVPLNVLVTSRL 560
           + +DM+E  G+ + K  PL VLV  R+
Sbjct: 491 NKVDMTETPGIVHAKATPLEVLVAPRI 513

BLAST of MC11g_new0047 vs. NCBI nr
Match: XP_022154118.1 (cytochrome P450 82A3-like [Momordica charantia])

HSP 1 Score: 1162 bits (3007), Expect = 0.0
Identity = 567/567 (100.00%), Postives = 567/567 (100.00%), Query Frame = 0

Query: 1   MIAYLNYFRKEKSLGVPKYYSLCKKLKGESNYGKRIRPTANNFRPPQIMESQFPPFNPTS 60
           MIAYLNYFRKEKSLGVPKYYSLCKKLKGESNYGKRIRPTANNFRPPQIMESQFPPFNPTS
Sbjct: 1   MIAYLNYFRKEKSLGVPKYYSLCKKLKGESNYGKRIRPTANNFRPPQIMESQFPPFNPTS 60

Query: 61  VATLVGIFVFCYYYLRRKWQERKQAPSPPGAWPIIGHLHLLHNAKLPHHTLGAMADKYGP 120
           VATLVGIFVFCYYYLRRKWQERKQAPSPPGAWPIIGHLHLLHNAKLPHHTLGAMADKYGP
Sbjct: 61  VATLVGIFVFCYYYLRRKWQERKQAPSPPGAWPIIGHLHLLHNAKLPHHTLGAMADKYGP 120

Query: 121 IFRLQLGCRSALVVSNWEMAKESMCINDAAAVSRPGISGTKHFSYDYAAFGLAPYSPYWR 180
           IFRLQLGCRSALVVSNWEMAKESMCINDAAAVSRPGISGTKHFSYDYAAFGLAPYSPYWR
Sbjct: 121 IFRLQLGCRSALVVSNWEMAKESMCINDAAAVSRPGISGTKHFSYDYAAFGLAPYSPYWR 180

Query: 181 EMRKITHLELLSNSRVDQVKNLMFAQIDMSLAELHAGSAQIPVEMKRWFGDVIVNMLLKI 240
           EMRKITHLELLSNSRVDQVKNLMFAQIDMSLAELHAGSAQIPVEMKRWFGDVIVNMLLKI
Sbjct: 181 EMRKITHLELLSNSRVDQVKNLMFAQIDMSLAELHAGSAQIPVEMKRWFGDVIVNMLLKI 240

Query: 241 IIGKRCVGPNAEGGEKGAKDLQMAIRDSFHLMGQGLLTDYIPLIGRLGFDGQVKVMESIA 300
           IIGKRCVGPNAEGGEKGAKDLQMAIRDSFHLMGQGLLTDYIPLIGRLGFDGQVKVMESIA
Sbjct: 241 IIGKRCVGPNAEGGEKGAKDLQMAIRDSFHLMGQGLLTDYIPLIGRLGFDGQVKVMESIA 300

Query: 301 KRFDVIMSEWLEEHKRKRDYGLGQNQDGDFMDSLISLLDGKGIQGYYDADTIIKATTLNM 360
           KRFDVIMSEWLEEHKRKRDYGLGQNQDGDFMDSLISLLDGKGIQGYYDADTIIKATTLNM
Sbjct: 301 KRFDVIMSEWLEEHKRKRDYGLGQNQDGDFMDSLISLLDGKGIQGYYDADTIIKATTLNM 360

Query: 361 IAGGTESTTVTLTWTMTLLLNNPHTLEMAQQELDAVVGRDRRVNESDISKLHYLQAVVKE 420
           IAGGTESTTVTLTWTMTLLLNNPHTLEMAQQELDAVVGRDRRVNESDISKLHYLQAVVKE
Sbjct: 361 IAGGTESTTVTLTWTMTLLLNNPHTLEMAQQELDAVVGRDRRVNESDISKLHYLQAVVKE 420

Query: 421 AMRMYPAGPLLGPREFSSDCVVAGYFVPRGTQLIPNIWKIQTDPRVWPNPFEFKPERFLT 480
           AMRMYPAGPLLGPREFSSDCVVAGYFVPRGTQLIPNIWKIQTDPRVWPNPFEFKPERFLT
Sbjct: 421 AMRMYPAGPLLGPREFSSDCVVAGYFVPRGTQLIPNIWKIQTDPRVWPNPFEFKPERFLT 480

Query: 481 THKDVDAKGNHFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQSFDVKSSSADLIDMSEN 540
           THKDVDAKGNHFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQSFDVKSSSADLIDMSEN
Sbjct: 481 THKDVDAKGNHFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQSFDVKSSSADLIDMSEN 540

Query: 541 FGMANEKVVPLNVLVTSRLPSHLHGFN 567
           FGMANEKVVPLNVLVTSRLPSHLHGFN
Sbjct: 541 FGMANEKVVPLNVLVTSRLPSHLHGFN 567

BLAST of MC11g_new0047 vs. NCBI nr
Match: XP_004147824.1 (cytochrome P450 CYP82D47 [Cucumis sativus] >KGN59910.1 hypothetical protein Csa_000849 [Cucumis sativus])

HSP 1 Score: 852 bits (2201), Expect = 1.21e-307
Identity = 409/532 (76.88%), Postives = 457/532 (85.90%), Query Frame = 0

Query: 48  IMESQFPPFNPTSVATLVGIFVFCYYYLRRKWQ-----ERKQAPSPPGAWPIIGHLHLLH 107
           ++E QFP FNP S+  LV +F    YYL +KWQ     E KQAP+PPGAWPIIGHLH+LH
Sbjct: 1   MVEFQFPFFNPKSITMLVFLFFISCYYLWQKWQQYKSTEPKQAPAPPGAWPIIGHLHMLH 60

Query: 108 NAKLPHHTLGAMADKYGPIFRLQLGCRSALVVSNWEMAKESMCINDAAAVSRPGISGTKH 167
           N KLPHH LGAMADKYGP+FRLQLG RSALVVS+WEMAKESMC+NDAAA SRPG+SGTKH
Sbjct: 61  NVKLPHHALGAMADKYGPLFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKH 120

Query: 168 FSYDYAAFGLAPYSPYWREMRKITHLELLSNSRVDQVKNLMFAQIDMSLAELHA------ 227
           FSYD+AAFGLAPYSPYWRE+RK+TH+ELLSN RVDQ KN MF ++  SL ELH       
Sbjct: 121 FSYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQK 180

Query: 228 -GSAQIPVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKGAKDLQMAIRDSFHLMGQG 287
            GS Q+ VEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEK AKD Q+AIRDSFHLMGQG
Sbjct: 181 DGSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQG 240

Query: 288 LLTDYIPLIGRLGFDGQVKVMESIAKRFDVIMSEWLEEHKRKRDYGLGQNQDGDFMDSLI 347
           LL DYIPLIGRLGF+GQVKVME+IA RFD+++ EWL+EHK  R       +DGDFMD+L+
Sbjct: 241 LLRDYIPLIGRLGFNGQVKVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGDFMDALV 300

Query: 348 SLLDGKGIQGYYDADTIIKATTLNMIAGGTESTTVTLTWTMTLLLNNPHTLEMAQQELDA 407
           SL DGK I+GYYD DTIIKATTLNM+AGGTESTTVTLTW M+LL+NNPH LE AQQELD 
Sbjct: 301 SLYDGKEIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELDT 360

Query: 408 VVGRDRRVNESDISKLHYLQAVVKEAMRMYPAGPLLGPREFSSDCVVAGYFVPRGTQLIP 467
           VVGRDR++ ESDI  L YL++++KE MRMYPAGPLLGPREF  DC+VAGYFVP+GTQLIP
Sbjct: 361 VVGRDRQLKESDIPNLVYLKSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIP 420

Query: 468 NIWKIQTDPRVWPNPFEFKPERFLTTHKDVDAKGNHFELIPFGSGRRGCPGVAFGLQMVH 527
           NIWKIQTDPRVWP+PFEFKPERFLTTHK+VD KGN+FELIPFGSGRRGCPG+AFGLQMVH
Sbjct: 421 NIWKIQTDPRVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVH 480

Query: 528 FTLAGFLQSFDVKSSSADLIDMSENFGMANEKVVPLNVLVTSRLPSHLHGFN 567
           F LAGFL SFDVK+ + + IDMSENFGMANEKVVPLNV VTSRLPSHL+  N
Sbjct: 481 FALAGFLHSFDVKNPTKEPIDMSENFGMANEKVVPLNVSVTSRLPSHLYTIN 532

BLAST of MC11g_new0047 vs. NCBI nr
Match: XP_022935740.1 (cytochrome P450 82A3-like [Cucurbita moschata])

HSP 1 Score: 848 bits (2190), Expect = 4.92e-306
Identity = 415/523 (79.35%), Postives = 455/523 (87.00%), Query Frame = 0

Query: 58  PTSVATLVGIF-VFCYYYLRRKWQ-----ERKQAPSPPGAWPIIGHLHLLHNAKLPHHTL 117
           P+S+  L+ +F +   YYL RKWQ     ERKQAPSPPGAWPIIGHLHLLHNAKLPHH L
Sbjct: 6   PSSITVLLLLFFITSCYYLWRKWQQHKVAERKQAPSPPGAWPIIGHLHLLHNAKLPHHAL 65

Query: 118 GAMADKYGPIFRLQLGCRSALVVSNWEMAKESMCINDAAAVSRPGISGTKHFSYDYAAFG 177
           GAMADKYGPIFRLQLG RSALVVS+WEMAKESMC NDAAA SRPG+SGTK+FSYD+AAFG
Sbjct: 66  GAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCFNDAAAASRPGVSGTKYFSYDFAAFG 125

Query: 178 LAPYSPYWREMRKITHLELLSNSRVDQVKNLMFAQIDMSLAELHAG-------SAQIPVE 237
           LAPYSPYWRE+RK+TH+ELLSN RV QVK+ M AQI+ SL ELH         S Q+ VE
Sbjct: 126 LAPYSPYWREIRKVTHIELLSNPRVHQVKSTMLAQINTSLTELHETWGAQKDESGQVAVE 185

Query: 238 MKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKGAKDLQMAIRDSFHLMGQGLLTDYIPLI 297
           MKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEK AKD+Q+AIRDSFHLMGQGLL DYIPLI
Sbjct: 186 MKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKEAKDIQLAIRDSFHLMGQGLLQDYIPLI 245

Query: 298 GRLGFDGQVKVMESIAKRFDVIMSEWLEEHKRKRDYGLGQNQDGDFMDSLISLLDGKGIQ 357
           GRLGF+GQV VM++IAKRFD+++SEWLEEHKR+R    G+N D DFMD+LISL +GK I+
Sbjct: 246 GRLGFNGQVPVMKNIAKRFDMVLSEWLEEHKRRRSASSGEN-DRDFMDALISLYNGKEIE 305

Query: 358 GYYDADTIIKATTLNMIAGGTESTTVTLTWTMTLLLNNPHTLEMAQQELDAVVGRDRRVN 417
           GYYD DTI KATTLNM AGGTESTTVTLTW M+LLLNNPH LE A QELDAVVGR+RRV 
Sbjct: 306 GYYDGDTITKATTLNMFAGGTESTTVTLTWAMSLLLNNPHVLEKAWQELDAVVGRERRVT 365

Query: 418 ESDISKLHYLQAVVKEAMRMYPAGPLLGPREFSSDCVVAGYFVPRGTQLIPNIWKIQTDP 477
           E D+ KL YLQA++KE MR+YPAGPLLGPREF  DCVV GYFVP+GTQLIPNIWKIQTDP
Sbjct: 366 EDDVGKLVYLQAIIKETMRLYPAGPLLGPREFHKDCVVGGYFVPKGTQLIPNIWKIQTDP 425

Query: 478 RVWPNPFEFKPERFLTTHKDVDAKGNHFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQS 537
           RVW  PFEFKPERFLTTHKDVD KGNHFELIPFGSGRRGCPGVAFGLQM+HFTLAGFLQ 
Sbjct: 426 RVWCEPFEFKPERFLTTHKDVDLKGNHFELIPFGSGRRGCPGVAFGLQMMHFTLAGFLQC 485

Query: 538 FDVKSSSADLIDMSENFGMANEKVVPLNVLVTSRLPSHLHGFN 567
           FDVK+ + D IDMSENFGMANEKV+PL+VLVTSRLPSHLH FN
Sbjct: 486 FDVKNPTKDPIDMSENFGMANEKVLPLHVLVTSRLPSHLHAFN 527

BLAST of MC11g_new0047 vs. NCBI nr
Match: XP_008464762.1 (PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo])

HSP 1 Score: 845 bits (2183), Expect = 6.65e-305
Identity = 406/532 (76.32%), Postives = 455/532 (85.53%), Query Frame = 0

Query: 48  IMESQFPPFNPTSVATLVGIFVFCYYYLRRKWQERK-----QAPSPPGAWPIIGHLHLLH 107
           ++E QFP FNP S+  L+  F    YYL RKW++ K     QAP+PPGA PIIGHLH+LH
Sbjct: 1   MIEFQFPFFNPKSITMLILWFFISCYYLWRKWKQYKSTNLKQAPAPPGALPIIGHLHMLH 60

Query: 108 NAKLPHHTLGAMADKYGPIFRLQLGCRSALVVSNWEMAKESMCINDAAAVSRPGISGTKH 167
           N KLPHH LGAMADKYGPIFRLQLG RSALVVS+WEMAKESMC+NDAAA SRP +SGTKH
Sbjct: 61  NVKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPAVSGTKH 120

Query: 168 FSYDYAAFGLAPYSPYWREMRKITHLELLSNSRVDQVKNLMFAQIDMSLAELHA------ 227
           FSYD+AAFGLAPY PYWRE+RK+TH+ELLSN RVDQ KN MF ++  SL ELH       
Sbjct: 121 FSYDFAAFGLAPYGPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLTELHETWAAQK 180

Query: 228 -GSAQIPVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKGAKDLQMAIRDSFHLMGQG 287
            GS Q+ VEMKRWFGDVIVNMLLKIIIGKRCVGPNA+GGEK AKD Q+AIRDSFHLMGQG
Sbjct: 181 DGSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNAKGGEKQAKDFQLAIRDSFHLMGQG 240

Query: 288 LLTDYIPLIGRLGFDGQVKVMESIAKRFDVIMSEWLEEHKRKRDYGLGQNQDGDFMDSLI 347
           LL DY+PLIG LGF+GQVKVME+IA RFDV++SEWL+EHKR R       +DGDFMD+L+
Sbjct: 241 LLRDYVPLIGWLGFNGQVKVMENIATRFDVVLSEWLDEHKRNRTSSCCGRRDGDFMDALV 300

Query: 348 SLLDGKGIQGYYDADTIIKATTLNMIAGGTESTTVTLTWTMTLLLNNPHTLEMAQQELDA 407
           SL DGKGI+GYYD DTIIKATTLNM+AGGTESTTVTLTW M+LLLNNPH LE AQQELD 
Sbjct: 301 SLYDGKGIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLLNNPHVLERAQQELDT 360

Query: 408 VVGRDRRVNESDISKLHYLQAVVKEAMRMYPAGPLLGPREFSSDCVVAGYFVPRGTQLIP 467
           VVGRDRR+ ESDI  L YLQ+++KE MRMYPAGPLLGPREF  DC+VAGYFVP+GTQLIP
Sbjct: 361 VVGRDRRLKESDIPNLVYLQSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIP 420

Query: 468 NIWKIQTDPRVWPNPFEFKPERFLTTHKDVDAKGNHFELIPFGSGRRGCPGVAFGLQMVH 527
           N+W+IQTDPRVWP+PF+FKPERFLTTHK+VD KGN+FELIPFGSGRRGCPG AFGLQMVH
Sbjct: 421 NLWRIQTDPRVWPDPFKFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGAAFGLQMVH 480

Query: 528 FTLAGFLQSFDVKSSSADLIDMSENFGMANEKVVPLNVLVTSRLPSHLHGFN 567
           F LAGFL SFDV++ + + IDMSENFGMANEKVVPLNVLVTSRLPSHL+  N
Sbjct: 481 FALAGFLHSFDVRNPTKEPIDMSENFGMANEKVVPLNVLVTSRLPSHLYTVN 532

BLAST of MC11g_new0047 vs. NCBI nr
Match: XP_023535455.1 (cytochrome P450 82A3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 844 bits (2181), Expect = 1.15e-304
Identity = 413/523 (78.97%), Postives = 456/523 (87.19%), Query Frame = 0

Query: 58  PTSVATLVGIF-VFCYYYLRRKWQ-----ERKQAPSPPGAWPIIGHLHLLHNAKLPHHTL 117
           P+S+  L+ +F +   YYL RKWQ     ERKQAPSPPGAWPIIGHLHLLHNAKLPHH L
Sbjct: 6   PSSITVLLHLFFITSCYYLWRKWQHHKVAERKQAPSPPGAWPIIGHLHLLHNAKLPHHAL 65

Query: 118 GAMADKYGPIFRLQLGCRSALVVSNWEMAKESMCINDAAAVSRPGISGTKHFSYDYAAFG 177
           GAMADKYGPIFRLQLG RSALVVS+WEMAKESMC+NDAAA SRPG+SGTK+FSYD+AAFG
Sbjct: 66  GAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKYFSYDFAAFG 125

Query: 178 LAPYSPYWREMRKITHLELLSNSRVDQVKNLMFAQIDMSLAELHAG-------SAQIPVE 237
           LAPYSPYWRE+RK+TH+ELLSN RV QVK+ M AQI+ SL ELH         S Q+ VE
Sbjct: 126 LAPYSPYWREIRKVTHMELLSNPRVHQVKSPMLAQINTSLTELHETWGAQKDESGQVAVE 185

Query: 238 MKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKGAKDLQMAIRDSFHLMGQGLLTDYIPLI 297
           MKRWFGDVI+NMLLKIIIGKRCVGPNAEGGEK AKD+Q+AIRDSFHLMGQGLL DYIPLI
Sbjct: 186 MKRWFGDVIMNMLLKIIIGKRCVGPNAEGGEKEAKDIQLAIRDSFHLMGQGLLQDYIPLI 245

Query: 298 GRLGFDGQVKVMESIAKRFDVIMSEWLEEHKRKRDYGLGQNQDGDFMDSLISLLDGKGIQ 357
           GRLGF+GQV VME+IAKRFD+++S+WLEEHKR+R    G+ +D DFMD+LISL +GK I+
Sbjct: 246 GRLGFNGQVPVMENIAKRFDMVLSQWLEEHKRRRSASSGE-KDRDFMDALISLYNGKEIE 305

Query: 358 GYYDADTIIKATTLNMIAGGTESTTVTLTWTMTLLLNNPHTLEMAQQELDAVVGRDRRVN 417
           GYYD D I KATTLNMIAGGTESTTVTLTW M+LLLNNPH LE A QELDAVVGR+RRV 
Sbjct: 306 GYYDGDIITKATTLNMIAGGTESTTVTLTWAMSLLLNNPHVLEKAWQELDAVVGRERRVT 365

Query: 418 ESDISKLHYLQAVVKEAMRMYPAGPLLGPREFSSDCVVAGYFVPRGTQLIPNIWKIQTDP 477
           E DISKL YLQA++KE MR+YPAGPLLGPREF  DCVV GYFVP+GTQLIPNIWKIQTDP
Sbjct: 366 EDDISKLVYLQAIIKETMRLYPAGPLLGPREFHKDCVVGGYFVPKGTQLIPNIWKIQTDP 425

Query: 478 RVWPNPFEFKPERFLTTHKDVDAKGNHFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQS 537
           RVW  PFEFKPERF+ THKDVD KGNHFELIPFGSGRRGCPGVAFGLQM+HFTLAGFLQ 
Sbjct: 426 RVWCEPFEFKPERFMRTHKDVDLKGNHFELIPFGSGRRGCPGVAFGLQMMHFTLAGFLQC 485

Query: 538 FDVKSSSADLIDMSENFGMANEKVVPLNVLVTSRLPSHLHGFN 567
           FDVK+ + D IDMSENFGMANEKV+PL+VLVTSRLPSHLH FN
Sbjct: 486 FDVKNPTKDPIDMSENFGMANEKVLPLHVLVTSRLPSHLHAFN 527

BLAST of MC11g_new0047 vs. ExPASy TrEMBL
Match: A0A6J1DJE5 (cytochrome P450 82A3-like OS=Momordica charantia OX=3673 GN=LOC111021426 PE=3 SV=1)

HSP 1 Score: 1162 bits (3007), Expect = 0.0
Identity = 567/567 (100.00%), Postives = 567/567 (100.00%), Query Frame = 0

Query: 1   MIAYLNYFRKEKSLGVPKYYSLCKKLKGESNYGKRIRPTANNFRPPQIMESQFPPFNPTS 60
           MIAYLNYFRKEKSLGVPKYYSLCKKLKGESNYGKRIRPTANNFRPPQIMESQFPPFNPTS
Sbjct: 1   MIAYLNYFRKEKSLGVPKYYSLCKKLKGESNYGKRIRPTANNFRPPQIMESQFPPFNPTS 60

Query: 61  VATLVGIFVFCYYYLRRKWQERKQAPSPPGAWPIIGHLHLLHNAKLPHHTLGAMADKYGP 120
           VATLVGIFVFCYYYLRRKWQERKQAPSPPGAWPIIGHLHLLHNAKLPHHTLGAMADKYGP
Sbjct: 61  VATLVGIFVFCYYYLRRKWQERKQAPSPPGAWPIIGHLHLLHNAKLPHHTLGAMADKYGP 120

Query: 121 IFRLQLGCRSALVVSNWEMAKESMCINDAAAVSRPGISGTKHFSYDYAAFGLAPYSPYWR 180
           IFRLQLGCRSALVVSNWEMAKESMCINDAAAVSRPGISGTKHFSYDYAAFGLAPYSPYWR
Sbjct: 121 IFRLQLGCRSALVVSNWEMAKESMCINDAAAVSRPGISGTKHFSYDYAAFGLAPYSPYWR 180

Query: 181 EMRKITHLELLSNSRVDQVKNLMFAQIDMSLAELHAGSAQIPVEMKRWFGDVIVNMLLKI 240
           EMRKITHLELLSNSRVDQVKNLMFAQIDMSLAELHAGSAQIPVEMKRWFGDVIVNMLLKI
Sbjct: 181 EMRKITHLELLSNSRVDQVKNLMFAQIDMSLAELHAGSAQIPVEMKRWFGDVIVNMLLKI 240

Query: 241 IIGKRCVGPNAEGGEKGAKDLQMAIRDSFHLMGQGLLTDYIPLIGRLGFDGQVKVMESIA 300
           IIGKRCVGPNAEGGEKGAKDLQMAIRDSFHLMGQGLLTDYIPLIGRLGFDGQVKVMESIA
Sbjct: 241 IIGKRCVGPNAEGGEKGAKDLQMAIRDSFHLMGQGLLTDYIPLIGRLGFDGQVKVMESIA 300

Query: 301 KRFDVIMSEWLEEHKRKRDYGLGQNQDGDFMDSLISLLDGKGIQGYYDADTIIKATTLNM 360
           KRFDVIMSEWLEEHKRKRDYGLGQNQDGDFMDSLISLLDGKGIQGYYDADTIIKATTLNM
Sbjct: 301 KRFDVIMSEWLEEHKRKRDYGLGQNQDGDFMDSLISLLDGKGIQGYYDADTIIKATTLNM 360

Query: 361 IAGGTESTTVTLTWTMTLLLNNPHTLEMAQQELDAVVGRDRRVNESDISKLHYLQAVVKE 420
           IAGGTESTTVTLTWTMTLLLNNPHTLEMAQQELDAVVGRDRRVNESDISKLHYLQAVVKE
Sbjct: 361 IAGGTESTTVTLTWTMTLLLNNPHTLEMAQQELDAVVGRDRRVNESDISKLHYLQAVVKE 420

Query: 421 AMRMYPAGPLLGPREFSSDCVVAGYFVPRGTQLIPNIWKIQTDPRVWPNPFEFKPERFLT 480
           AMRMYPAGPLLGPREFSSDCVVAGYFVPRGTQLIPNIWKIQTDPRVWPNPFEFKPERFLT
Sbjct: 421 AMRMYPAGPLLGPREFSSDCVVAGYFVPRGTQLIPNIWKIQTDPRVWPNPFEFKPERFLT 480

Query: 481 THKDVDAKGNHFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQSFDVKSSSADLIDMSEN 540
           THKDVDAKGNHFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQSFDVKSSSADLIDMSEN
Sbjct: 481 THKDVDAKGNHFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQSFDVKSSSADLIDMSEN 540

Query: 541 FGMANEKVVPLNVLVTSRLPSHLHGFN 567
           FGMANEKVVPLNVLVTSRLPSHLHGFN
Sbjct: 541 FGMANEKVVPLNVLVTSRLPSHLHGFN 567

BLAST of MC11g_new0047 vs. ExPASy TrEMBL
Match: A0A0A0LD43 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852590 PE=3 SV=1)

HSP 1 Score: 852 bits (2201), Expect = 5.86e-308
Identity = 409/532 (76.88%), Postives = 457/532 (85.90%), Query Frame = 0

Query: 48  IMESQFPPFNPTSVATLVGIFVFCYYYLRRKWQ-----ERKQAPSPPGAWPIIGHLHLLH 107
           ++E QFP FNP S+  LV +F    YYL +KWQ     E KQAP+PPGAWPIIGHLH+LH
Sbjct: 1   MVEFQFPFFNPKSITMLVFLFFISCYYLWQKWQQYKSTEPKQAPAPPGAWPIIGHLHMLH 60

Query: 108 NAKLPHHTLGAMADKYGPIFRLQLGCRSALVVSNWEMAKESMCINDAAAVSRPGISGTKH 167
           N KLPHH LGAMADKYGP+FRLQLG RSALVVS+WEMAKESMC+NDAAA SRPG+SGTKH
Sbjct: 61  NVKLPHHALGAMADKYGPLFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKH 120

Query: 168 FSYDYAAFGLAPYSPYWREMRKITHLELLSNSRVDQVKNLMFAQIDMSLAELHA------ 227
           FSYD+AAFGLAPYSPYWRE+RK+TH+ELLSN RVDQ KN MF ++  SL ELH       
Sbjct: 121 FSYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQK 180

Query: 228 -GSAQIPVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKGAKDLQMAIRDSFHLMGQG 287
            GS Q+ VEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEK AKD Q+AIRDSFHLMGQG
Sbjct: 181 DGSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQG 240

Query: 288 LLTDYIPLIGRLGFDGQVKVMESIAKRFDVIMSEWLEEHKRKRDYGLGQNQDGDFMDSLI 347
           LL DYIPLIGRLGF+GQVKVME+IA RFD+++ EWL+EHK  R       +DGDFMD+L+
Sbjct: 241 LLRDYIPLIGRLGFNGQVKVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGDFMDALV 300

Query: 348 SLLDGKGIQGYYDADTIIKATTLNMIAGGTESTTVTLTWTMTLLLNNPHTLEMAQQELDA 407
           SL DGK I+GYYD DTIIKATTLNM+AGGTESTTVTLTW M+LL+NNPH LE AQQELD 
Sbjct: 301 SLYDGKEIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELDT 360

Query: 408 VVGRDRRVNESDISKLHYLQAVVKEAMRMYPAGPLLGPREFSSDCVVAGYFVPRGTQLIP 467
           VVGRDR++ ESDI  L YL++++KE MRMYPAGPLLGPREF  DC+VAGYFVP+GTQLIP
Sbjct: 361 VVGRDRQLKESDIPNLVYLKSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIP 420

Query: 468 NIWKIQTDPRVWPNPFEFKPERFLTTHKDVDAKGNHFELIPFGSGRRGCPGVAFGLQMVH 527
           NIWKIQTDPRVWP+PFEFKPERFLTTHK+VD KGN+FELIPFGSGRRGCPG+AFGLQMVH
Sbjct: 421 NIWKIQTDPRVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVH 480

Query: 528 FTLAGFLQSFDVKSSSADLIDMSENFGMANEKVVPLNVLVTSRLPSHLHGFN 567
           F LAGFL SFDVK+ + + IDMSENFGMANEKVVPLNV VTSRLPSHL+  N
Sbjct: 481 FALAGFLHSFDVKNPTKEPIDMSENFGMANEKVVPLNVSVTSRLPSHLYTIN 532

BLAST of MC11g_new0047 vs. ExPASy TrEMBL
Match: A0A6J1FBI1 (cytochrome P450 82A3-like OS=Cucurbita moschata OX=3662 GN=LOC111442563 PE=3 SV=1)

HSP 1 Score: 848 bits (2190), Expect = 2.38e-306
Identity = 415/523 (79.35%), Postives = 455/523 (87.00%), Query Frame = 0

Query: 58  PTSVATLVGIF-VFCYYYLRRKWQ-----ERKQAPSPPGAWPIIGHLHLLHNAKLPHHTL 117
           P+S+  L+ +F +   YYL RKWQ     ERKQAPSPPGAWPIIGHLHLLHNAKLPHH L
Sbjct: 6   PSSITVLLLLFFITSCYYLWRKWQQHKVAERKQAPSPPGAWPIIGHLHLLHNAKLPHHAL 65

Query: 118 GAMADKYGPIFRLQLGCRSALVVSNWEMAKESMCINDAAAVSRPGISGTKHFSYDYAAFG 177
           GAMADKYGPIFRLQLG RSALVVS+WEMAKESMC NDAAA SRPG+SGTK+FSYD+AAFG
Sbjct: 66  GAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCFNDAAAASRPGVSGTKYFSYDFAAFG 125

Query: 178 LAPYSPYWREMRKITHLELLSNSRVDQVKNLMFAQIDMSLAELHAG-------SAQIPVE 237
           LAPYSPYWRE+RK+TH+ELLSN RV QVK+ M AQI+ SL ELH         S Q+ VE
Sbjct: 126 LAPYSPYWREIRKVTHIELLSNPRVHQVKSTMLAQINTSLTELHETWGAQKDESGQVAVE 185

Query: 238 MKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKGAKDLQMAIRDSFHLMGQGLLTDYIPLI 297
           MKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEK AKD+Q+AIRDSFHLMGQGLL DYIPLI
Sbjct: 186 MKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKEAKDIQLAIRDSFHLMGQGLLQDYIPLI 245

Query: 298 GRLGFDGQVKVMESIAKRFDVIMSEWLEEHKRKRDYGLGQNQDGDFMDSLISLLDGKGIQ 357
           GRLGF+GQV VM++IAKRFD+++SEWLEEHKR+R    G+N D DFMD+LISL +GK I+
Sbjct: 246 GRLGFNGQVPVMKNIAKRFDMVLSEWLEEHKRRRSASSGEN-DRDFMDALISLYNGKEIE 305

Query: 358 GYYDADTIIKATTLNMIAGGTESTTVTLTWTMTLLLNNPHTLEMAQQELDAVVGRDRRVN 417
           GYYD DTI KATTLNM AGGTESTTVTLTW M+LLLNNPH LE A QELDAVVGR+RRV 
Sbjct: 306 GYYDGDTITKATTLNMFAGGTESTTVTLTWAMSLLLNNPHVLEKAWQELDAVVGRERRVT 365

Query: 418 ESDISKLHYLQAVVKEAMRMYPAGPLLGPREFSSDCVVAGYFVPRGTQLIPNIWKIQTDP 477
           E D+ KL YLQA++KE MR+YPAGPLLGPREF  DCVV GYFVP+GTQLIPNIWKIQTDP
Sbjct: 366 EDDVGKLVYLQAIIKETMRLYPAGPLLGPREFHKDCVVGGYFVPKGTQLIPNIWKIQTDP 425

Query: 478 RVWPNPFEFKPERFLTTHKDVDAKGNHFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQS 537
           RVW  PFEFKPERFLTTHKDVD KGNHFELIPFGSGRRGCPGVAFGLQM+HFTLAGFLQ 
Sbjct: 426 RVWCEPFEFKPERFLTTHKDVDLKGNHFELIPFGSGRRGCPGVAFGLQMMHFTLAGFLQC 485

Query: 538 FDVKSSSADLIDMSENFGMANEKVVPLNVLVTSRLPSHLHGFN 567
           FDVK+ + D IDMSENFGMANEKV+PL+VLVTSRLPSHLH FN
Sbjct: 486 FDVKNPTKDPIDMSENFGMANEKVLPLHVLVTSRLPSHLHAFN 527

BLAST of MC11g_new0047 vs. ExPASy TrEMBL
Match: A0A1S3CMR1 (cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502568 PE=3 SV=1)

HSP 1 Score: 845 bits (2183), Expect = 3.22e-305
Identity = 406/532 (76.32%), Postives = 455/532 (85.53%), Query Frame = 0

Query: 48  IMESQFPPFNPTSVATLVGIFVFCYYYLRRKWQERK-----QAPSPPGAWPIIGHLHLLH 107
           ++E QFP FNP S+  L+  F    YYL RKW++ K     QAP+PPGA PIIGHLH+LH
Sbjct: 1   MIEFQFPFFNPKSITMLILWFFISCYYLWRKWKQYKSTNLKQAPAPPGALPIIGHLHMLH 60

Query: 108 NAKLPHHTLGAMADKYGPIFRLQLGCRSALVVSNWEMAKESMCINDAAAVSRPGISGTKH 167
           N KLPHH LGAMADKYGPIFRLQLG RSALVVS+WEMAKESMC+NDAAA SRP +SGTKH
Sbjct: 61  NVKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPAVSGTKH 120

Query: 168 FSYDYAAFGLAPYSPYWREMRKITHLELLSNSRVDQVKNLMFAQIDMSLAELHA------ 227
           FSYD+AAFGLAPY PYWRE+RK+TH+ELLSN RVDQ KN MF ++  SL ELH       
Sbjct: 121 FSYDFAAFGLAPYGPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLTELHETWAAQK 180

Query: 228 -GSAQIPVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKGAKDLQMAIRDSFHLMGQG 287
            GS Q+ VEMKRWFGDVIVNMLLKIIIGKRCVGPNA+GGEK AKD Q+AIRDSFHLMGQG
Sbjct: 181 DGSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNAKGGEKQAKDFQLAIRDSFHLMGQG 240

Query: 288 LLTDYIPLIGRLGFDGQVKVMESIAKRFDVIMSEWLEEHKRKRDYGLGQNQDGDFMDSLI 347
           LL DY+PLIG LGF+GQVKVME+IA RFDV++SEWL+EHKR R       +DGDFMD+L+
Sbjct: 241 LLRDYVPLIGWLGFNGQVKVMENIATRFDVVLSEWLDEHKRNRTSSCCGRRDGDFMDALV 300

Query: 348 SLLDGKGIQGYYDADTIIKATTLNMIAGGTESTTVTLTWTMTLLLNNPHTLEMAQQELDA 407
           SL DGKGI+GYYD DTIIKATTLNM+AGGTESTTVTLTW M+LLLNNPH LE AQQELD 
Sbjct: 301 SLYDGKGIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLLNNPHVLERAQQELDT 360

Query: 408 VVGRDRRVNESDISKLHYLQAVVKEAMRMYPAGPLLGPREFSSDCVVAGYFVPRGTQLIP 467
           VVGRDRR+ ESDI  L YLQ+++KE MRMYPAGPLLGPREF  DC+VAGYFVP+GTQLIP
Sbjct: 361 VVGRDRRLKESDIPNLVYLQSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIP 420

Query: 468 NIWKIQTDPRVWPNPFEFKPERFLTTHKDVDAKGNHFELIPFGSGRRGCPGVAFGLQMVH 527
           N+W+IQTDPRVWP+PF+FKPERFLTTHK+VD KGN+FELIPFGSGRRGCPG AFGLQMVH
Sbjct: 421 NLWRIQTDPRVWPDPFKFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGAAFGLQMVH 480

Query: 528 FTLAGFLQSFDVKSSSADLIDMSENFGMANEKVVPLNVLVTSRLPSHLHGFN 567
           F LAGFL SFDV++ + + IDMSENFGMANEKVVPLNVLVTSRLPSHL+  N
Sbjct: 481 FALAGFLHSFDVRNPTKEPIDMSENFGMANEKVVPLNVLVTSRLPSHLYTVN 532

BLAST of MC11g_new0047 vs. ExPASy TrEMBL
Match: A0A6J1IGS1 (cytochrome P450 82A3-like OS=Cucurbita maxima OX=3661 GN=LOC111476809 PE=3 SV=1)

HSP 1 Score: 839 bits (2168), Expect = 5.12e-303
Identity = 411/523 (78.59%), Postives = 454/523 (86.81%), Query Frame = 0

Query: 58  PTSVATLVGIF-VFCYYYLRRKWQE-----RKQAPSPPGAWPIIGHLHLLHNAKLPHHTL 117
           P+S+  L+ +F +   YYL RKWQ+     RKQAPSPPGAWPIIGHLHLLHNAKLPHH L
Sbjct: 6   PSSIIVLLHLFFITSCYYLWRKWQQHKLADRKQAPSPPGAWPIIGHLHLLHNAKLPHHAL 65

Query: 118 GAMADKYGPIFRLQLGCRSALVVSNWEMAKESMCINDAAAVSRPGISGTKHFSYDYAAFG 177
           GAMADKYGPIF LQLG RSALVVS+WEMAKESMC+NDAAA SRPG+SGTK+FSYD+AAFG
Sbjct: 66  GAMADKYGPIFSLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKYFSYDFAAFG 125

Query: 178 LAPYSPYWREMRKITHLELLSNSRVDQVKNLMFAQIDMSLAELHAG-------SAQIPVE 237
           LAPYSPYWRE+RK+TH+ELLSN RV +V + M A+I+ SL ELH         S Q+ VE
Sbjct: 126 LAPYSPYWREIRKVTHMELLSNPRVYKVASTMLAEINTSLTELHETWGAQKDESGQVAVE 185

Query: 238 MKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKGAKDLQMAIRDSFHLMGQGLLTDYIPLI 297
           MKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEK AKD+Q+AIRDSFHLMGQGLL DYIPLI
Sbjct: 186 MKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKEAKDIQLAIRDSFHLMGQGLLQDYIPLI 245

Query: 298 GRLGFDGQVKVMESIAKRFDVIMSEWLEEHKRKRDYGLGQNQDGDFMDSLISLLDGKGIQ 357
           GRLGF+GQV VME+IAKRFD+++S+WLEEHKR+R    G+ +D DFMD+LISL +GKGI+
Sbjct: 246 GRLGFNGQVPVMENIAKRFDMVLSQWLEEHKRRRSASSGE-KDRDFMDALISLHNGKGIE 305

Query: 358 GYYDADTIIKATTLNMIAGGTESTTVTLTWTMTLLLNNPHTLEMAQQELDAVVGRDRRVN 417
           GYYD DTI KATTLNMIAGGTESTTVTLTW M+LLLNNPH LE A QELDAVVGR+RRV 
Sbjct: 306 GYYDGDTITKATTLNMIAGGTESTTVTLTWAMSLLLNNPHVLEKAWQELDAVVGRERRVT 365

Query: 418 ESDISKLHYLQAVVKEAMRMYPAGPLLGPREFSSDCVVAGYFVPRGTQLIPNIWKIQTDP 477
           E DISKL YLQA++KE MR+YPAGPLLGPREF  DCVV GYFVP+GTQLIPNIWKIQTDP
Sbjct: 366 EDDISKLVYLQAIIKETMRLYPAGPLLGPREFHKDCVVGGYFVPKGTQLIPNIWKIQTDP 425

Query: 478 RVWPNPFEFKPERFLTTHKDVDAKGNHFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQS 537
           RVW  PFEFKPERFLTTHKDVD KGNHFELIPFGSGRRGCP  AFGLQM+HFTLAGFLQ 
Sbjct: 426 RVWCEPFEFKPERFLTTHKDVDLKGNHFELIPFGSGRRGCPAAAFGLQMMHFTLAGFLQC 485

Query: 538 FDVKSSSADLIDMSENFGMANEKVVPLNVLVTSRLPSHLHGFN 567
           FDVK+ + D IDMSENFGMANEKVVPL+VLVTSRLPSHLH  N
Sbjct: 486 FDVKNPTKDPIDMSENFGMANEKVVPLHVLVTSRLPSHLHALN 527

BLAST of MC11g_new0047 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 463.4 bits (1191), Expect = 2.6e-130
Identity = 234/512 (45.70%), Postives = 329/512 (64.26%), Query Frame = 0

Query: 67  IFVFCYYYLRRKWQERKQAPSPPGAWPIIGHLHLL-HNAKLPHHTLGAMADKYGPIFRLQ 126
           +FVF   + + K  +  +AP+P GAWPIIGHLHLL    +L + TLG MAD YGP   LQ
Sbjct: 14  VFVFIALFKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSLQ 73

Query: 127 LGCRSALVVSNWEMAKESMCINDAAAVSRPGISGTKHFSYDYAAFGLAPYSPYWREMRKI 186
           LG   A VVS++E+AK+   +ND A  SRP  +  KH  Y++A FG APYS +WREMRKI
Sbjct: 74  LGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWREMRKI 133

Query: 187 THLELLSNSRVDQVKNLMFAQIDMSLAELHA------GSAQIPVEMKRWFGDVIVNMLLK 246
             +ELLSN R+  +K++  ++I M + +L++      G+  + V++K W  D+ +NM+++
Sbjct: 134 ATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKPVMVDLKSWLEDMTLNMIVR 193

Query: 247 IIIGKRCVGPNAEGGEKGAKDLQ------MAIRDSFHLMGQGLLTDYIPLIGRLGFDGQV 306
           ++ GKR  G    GG   ++D +       AI   FHL+G   ++D  P +      G  
Sbjct: 194 MVAGKRYFG---GGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDLQGHE 253

Query: 307 KVMESIAKRFDVIMSEWLEEHKRKRDYGLGQNQDGDFMDSLISLLD-GKGIQGYYDADTI 366
           K M+      DVI+  W+E H+++R +   +  D DF+D ++SL + GK     YDA+T 
Sbjct: 254 KEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMSLAEQGKLSHLQYDANTS 313

Query: 367 IKATTLNMIAGGTESTTVTLTWTMTLLLNNPHTLEMAQQELDAVVGRDRRVNESDISKLH 426
           IK+T L +I GG++++  TLTW ++LLLNN   L+ AQ E+D  VGRDR V +SDI  L 
Sbjct: 314 IKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVEDSDIENLV 373

Query: 427 YLQAVVKEAMRMYPAGPLLGPREFSSDCVVAGYFVPRGTQLIPNIWKIQTDPRVWPNPFE 486
           YLQA++KE +R+YPAGPLLGPRE   DC VAGY+VP GT+LI N+WKIQ DP+V+  P E
Sbjct: 374 YLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPKVYMEPNE 433

Query: 487 FKPERFLTTH-KDVDAKGNHFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQSFDVKSSS 546
           F+PERF+T   K+ D +G +FEL+PFGSGRR CPG +  +Q++H  LA FL SFDVK+  
Sbjct: 434 FRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLHSFDVKTVM 493

Query: 547 ADLIDMSENFGMANEKVVPLNVLVTSRLPSHL 564
              +DMSEN G+   K  PL VL++ R+   L
Sbjct: 494 DMPVDMSENPGLTIPKATPLEVLISPRIKEEL 522

BLAST of MC11g_new0047 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 457.2 bits (1175), Expect = 1.9e-128
Identity = 230/509 (45.19%), Postives = 330/509 (64.83%), Query Frame = 0

Query: 67  IFVFCYYYLRRKWQERKQAPSPPGAWPIIGHLHLLH-NAKLPHHTLGAMADKYGPIFRLQ 126
           +FVF   + + K  +  +AP+P GAWPIIGHLHLL    +L + TLG MAD+YGP   L+
Sbjct: 14  VFVFIALFKKSKKPKHVKAPAPSGAWPIIGHLHLLSGKEQLLYRTLGKMADQYGPAMSLR 73

Query: 127 LGCRSALVVSNWEMAKESMCINDAAAVSRPGISGTKHFSYDYAAFGLAPYSPYWREMRKI 186
           LG     VVS++E+AK+   +ND A  SRP  +  KH  YD A FG APYS +WREMRKI
Sbjct: 74  LGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFAPYSAFWREMRKI 133

Query: 187 THLELLSNSRVDQVKNLMFAQIDMSLAELHA------GSAQIPVEMKRWFGDVIVNMLLK 246
             LELLSN R+  +K++  ++I M + +L++      GS  + V++K W  D+ +NM+++
Sbjct: 134 ATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSEPVMVDLKSWLEDMSLNMMVR 193

Query: 247 IIIGKRCVGPNAEGGE--KGAKDLQMAIRDSFHLMGQGLLTDYIPLIGRLGFDGQVKVME 306
           ++ GKR  G  +   E  + A+  +  + + FHL+G   ++D  P +G   F G  K M+
Sbjct: 194 MVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPKLGWFDFQGHEKEMK 253

Query: 307 SIAKRFDVIMSEWLEEHKRKRDYGLGQNQDGDFMDSLISLLD-GKGIQGYYDADTIIKAT 366
              +  DVI+  W+E H+++R     ++ D DF+D ++SL + GK     +DA T IK+T
Sbjct: 254 QTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKFSHLQHDAITSIKST 313

Query: 367 TLNMIAGGTESTTVTLTWTMTLLLNNPHTLEMAQQELDAVVGRDRRVNESDISKLHYLQA 426
            L +I GG+E++  TLTW ++LLLNN   L+ AQ E+D  VGRDR V +SDI  L Y+QA
Sbjct: 314 CLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVEDSDIENLVYIQA 373

Query: 427 VVKEAMRMYPAGPLLGPREFSSDCVVAGYFVPRGTQLIPNIWKIQTDPRVWPNPFEFKPE 486
           ++KE +R+YPAGPLLG RE   DC VAGY V RGT+++ N+WKIQ DPRV+  P EF+PE
Sbjct: 374 IIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPRVYMEPNEFRPE 433

Query: 487 RFLTTH-KDVDAKGNHFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQSFDVKSSSADLI 546
           RF+T   K+ D +G +FEL+PFGSGRR CPG +  +Q++H  LA FLQSFDVK+     +
Sbjct: 434 RFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQSFDVKTVMDMPV 493

Query: 547 DMSENFGMANEKVVPLNVLVTSRLPSHLH 565
           DM+E+ G+   K  PL +L++ RL   L+
Sbjct: 494 DMTESPGLTIPKATPLEILISPRLKEGLY 522

BLAST of MC11g_new0047 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 417.9 bits (1073), Expect = 1.3e-116
Identity = 224/509 (44.01%), Postives = 311/509 (61.10%), Query Frame = 0

Query: 67  IFVFCYYYLRRKWQERKQAPSPPGAWPIIGHLHLL-HNAKLPHHTLGAMADKYGPIFRLQ 126
           +FVF   + + K  +  +AP+P GAWPIIGHLHLL    +L + TLG MAD YGP   L+
Sbjct: 14  VFVFIALFKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSLR 73

Query: 127 LGCRSALVVSNWEMAKESMCINDAAAVSRPGISGTKHFSYDYAAFGLAPYSPYWREMRKI 186
           LG     V S++E+AK+   +ND A  S    +  KH  Y            +W EMRKI
Sbjct: 74  LGSSETFVGSSFEVAKDCFTVNDKALASLM-TAAAKHMGY-----------VFWLEMRKI 133

Query: 187 THLELLSNSRVDQVKNLMFAQIDMSLAELHA------GSAQIPVEMKRWFGDVIVNMLLK 246
             +ELLSN R+  + N+  ++I M + +L++      GS  + V++K W  D+I NM+++
Sbjct: 134 AMIELLSNRRLQMLNNVRVSEISMGVKDLYSLWVKKGGSEPVMVDLKSWLEDMIANMIMR 193

Query: 247 IIIGKRCV-GPNAEGGE--KGAKDLQMAIRDSFHLMGQGLLTDYIPLIGRLGFDGQVKVM 306
           ++ GKR   G  AE  E  + A+  +  I   FHL+G   ++D  P +G L   G  K M
Sbjct: 194 MVAGKRYFGGGGAESSEHTEEARQWRKGIAKFFHLVGIFTVSDAFPKLGWLDLQGHEKEM 253

Query: 307 ESIAKRFDVIMSEWLEEHKRKRDYGLGQNQDGDFMDSLISLLD-GKGIQGYYDADTIIKA 366
           +   +  DVI+  W+E H+++R     ++ D DF+D ++SL + GK     YDA+T IK 
Sbjct: 254 KQTRRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKLSHLQYDANTCIKT 313

Query: 367 TTLNMIAGGTESTTVTLTWTMTLLLNNPHTLEMAQQELDAVVGRDRRVNESDISKLHYLQ 426
           T L +I GG+E++  TLTW ++LLLNN   L+  Q E+D  VGRDR V +SDI  L YLQ
Sbjct: 314 TCLALILGGSETSPSTLTWAISLLLNNKDMLKKVQDEIDIHVGRDRNVEDSDIKNLVYLQ 373

Query: 427 AVVKEAMRMYPAGPLLGPREFSSDCVVAGYFVPRGTQLIPNIWKIQTDPRVWPNPFEFKP 486
           A++KE +R+YPA PLLG RE   DC VAGY VP GT+LI N+WKIQ DP+V+  P EF+P
Sbjct: 374 AIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIVNVWKIQRDPKVYMEPNEFRP 433

Query: 487 ERFLTTH-KDVDAKGNHFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQSFDVKSSSADL 546
           ERF+T   KD D +G +FEL+PFGSGRR CPG +  +QM+H  LA FL SF+VK+     
Sbjct: 434 ERFITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAMQMLHLGLARFLHSFEVKTVLDRP 493

Query: 547 IDMSENFGMANEKVVPLNVLVTSRLPSHL 564
           +DMSE+ G+   K  PL VL+  RL   L
Sbjct: 494 VDMSESPGLTITKATPLEVLINPRLKREL 510

BLAST of MC11g_new0047 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 366.3 bits (939), Expect = 4.4e-101
Identity = 197/498 (39.56%), Postives = 284/498 (57.03%), Query Frame = 0

Query: 77  RKWQERKQAPSPPGAWPIIGHLHLLHNAKLPHHTLGAMADKYGPIFRLQLGCRSALVVSN 136
           R   ++  AP  PGAWP++GHLHL       H T GAMAD YGP+F  +LG    +++++
Sbjct: 26  RPKNKKSTAPMVPGAWPLLGHLHLFDTVNPTHVTFGAMADVYGPVFMAKLGSIKVMIINS 85

Query: 137 WEMAKESMCINDAAAVSRPGISGTKHFSYDYAAFGLAPYSPYWREMRKITHLELLSNSRV 196
            E+AKE   ++D   + RP ++ +K   Y+ +    +PY  YWRE+RKI   EL S S V
Sbjct: 86  KEVAKEIYTVHD-KLLERPELTASKLLGYNDSFLTFSPYGLYWREIRKIAVSELFSTSGV 145

Query: 197 DQVKNLMFAQIDMSLAELHA-------GSAQIPVEMKRWFGDVIVNMLLKIIIGKRCVGP 256
           D        + D++   L+            + V+MK+ F D+  N+ L ++ GKR  G 
Sbjct: 146 DMHMVSRAREADLAFRALYVRWEKRGKPKEGVLVDMKQEFIDLTANISLMMVSGKRYFGE 205

Query: 257 NAEGGEKGAKDLQMAIRDSFHLMGQGLLTDYIPLIGRLGFDGQVKVMESIAKRFDVIMSE 316
           N     K A+     IR+        LL+D  P++G L +  + + M+  AK  D +   
Sbjct: 206 NPNCEVKEARRCGKLIREFLDYFALFLLSDVAPVLGFLDWKTK-RGMKRTAKGLDKVAEG 265

Query: 317 WLEEHKRKR-DYGLGQNQDGDFMDSLISLLDGKGIQGYYDADTIIKATTLNMIAGGTEST 376
           W+EEHK KR D+G  +N   D++D LI +L    I G  D  T IKA  LN++  G+E+ 
Sbjct: 266 WIEEHKNKRSDHGRSEN---DYLDILIKILGQDKIPGLSDTHTKIKALCLNLVLAGSETA 325

Query: 377 TVTLTWTMTLLLNNPHTLEMAQQELDAVVGRDRRVNESDISKLHYLQAVVKEAMRMYPAG 436
            V L W ++LLLNNPH L  AQ+ELD+ +G++R V E DI  L YLQA+VKE  R+YP  
Sbjct: 326 IVVLVWAVSLLLNNPHVLRKAQEELDSKIGKERVVEELDIKDLVYLQAIVKETFRLYPPV 385

Query: 437 PLLGPREFSSDCVVA--GYFVPRGTQLIPNIWKIQTDPRVWPNPFEFKPERFLTTHKDVD 496
           PL+  R    D  +A     VP GTQL+ + WKI  DP VW NP +F+PERFLT+++++D
Sbjct: 386 PLVAYRAVVEDFDIAFCKCHVPAGTQLMVSAWKIHRDPNVWSNPEQFEPERFLTSNRELD 445

Query: 497 AKGNHFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQSFDVKSSSADLIDMSENFGMANE 556
             G  ++  PFG GRR CP +  G++MVH+ L  FL SFD+   S+  +DM+E+ G+ N 
Sbjct: 446 VGGQSYKFFPFGLGRRSCPAIPLGMRMVHYLLVRFLHSFDLARPSSQDVDMTESNGLVNH 505

Query: 557 KVVPLNVLVTSRLPSHLH 565
           K  PL V +  RL   L+
Sbjct: 506 KATPLEVNIIPRLHKSLY 518

BLAST of MC11g_new0047 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 361.3 bits (926), Expect = 1.4e-99
Identity = 195/505 (38.61%), Postives = 296/505 (58.61%), Query Frame = 0

Query: 65  VGIFVFCYYYLRRKWQ----ERKQAPSPPGAWPIIGHLHLLHNAKLPHHTLGAMADKYGP 124
           + + +F Y +LR++      +    P P GA P+ GHLHLL   KL    L AM+ K+GP
Sbjct: 15  LALVIFGYIFLRKQLSRCEVDSSTIPEPLGALPLFGHLHLLRGKKLLCKKLAAMSQKHGP 74

Query: 125 IFRLQLGCRSALVVSNWEMAKESMCINDAAAVSRPGISGTKHFSYDYAAFGLAPYSPYWR 184
           IF L+LG    +V S+ +  K+    ND A  +RP I+  ++  Y+ A+  LAPY  YWR
Sbjct: 75  IFSLKLGFYRLVVASDPKTVKDCFTTNDLATATRPNIAFGRYVGYNNASLTLAPYGDYWR 134

Query: 185 EMRKITHLELLSNSRVDQVKNLMFAQIDMSLAELHAGSAQIP-VEMKRWFGDVIVNMLLK 244
           E+RKI  + L SN  ++ + ++  ++++  +  L+ G+     V++   F  +  N++L+
Sbjct: 135 ELRKIVTVHLFSNHSIEMLGHIRSSEVNTLIKHLYKGNGGTSIVKIDMLFEFLTFNIILR 194

Query: 245 IIIGKRCVGPNAEGGEKGAKDLQMAIRDSFHLMGQGLLTDYIPLIGRLGFDGQVKVMESI 304
            ++GKR         E   K+   A++   +L    ++ D IP +G L F  +   M+ +
Sbjct: 195 KMVGKRIGFGEVNSDEWRYKE---ALKHCEYLAVIPMIGDVIPWLGWLDF-AKNSQMKRL 254

Query: 305 AKRFDVIMSEWLEEHKRKRDYGLGQNQDGDFMDSLISLLDGKGIQGYYDADTIIKATTLN 364
            K  D + ++WL EH +KR     ++Q+   MD L+ +L    +   +  D I+KAT L 
Sbjct: 255 FKELDSVNTKWLHEHLKKRSRN-EKDQERTIMDLLLDILPEDIVISGHVRDVIVKATILA 314

Query: 365 MIAGGTESTTVTLTWTMTLLLNNPHTLEMAQQELDAVVGRDRRVNESDISKLHYLQAVVK 424
           +   G++ST++TLTW ++LLLNNP  LE AQ+E+D  VG+ R + ESDI  L YLQA+VK
Sbjct: 315 LTLTGSDSTSITLTWAVSLLLNNPAALEAAQEEIDNSVGKGRWIEESDIQNLKYLQAIVK 374

Query: 425 EAMRMYPAGPLLGPREFSSDCVVAGYFVPRGTQLIPNIWKIQTDPRVWPNPFEFKPERFL 484
           E  R+YP  PL G RE   DC V GY V +GT+L+ NIWK+  DP++WP+P  FKPERF+
Sbjct: 375 ETHRLYPPAPLTGIREAREDCFVGGYRVEKGTRLLVNIWKLHRDPKIWPDPKTFKPERFM 434

Query: 485 TTHKDVDAKGNHFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQSFDVKSSSADLIDMSE 544
                 +   ++FE IPFGSGRR CPGV  GL++VHF LA  LQ F++   S + +DM+E
Sbjct: 435 EDKSQCEK--SNFEYIPFGSGRRSCPGVNLGLRVVHFVLARLLQGFELHKVSDEPLDMAE 494

Query: 545 NFGMANEKVVPLNVLVTSRLPSHLH 565
             G+A  K+ P+ V+V  RL   L+
Sbjct: 495 GPGLALPKINPVEVVVMPRLDPKLY 512