MC11g1454 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC11g1454
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Threonylcarbamoyladenosine tRNA methylthiotransferase
Location	MC11: 19444596 .. 19456691 (+)
RNA-Seq Expression	MC11g1454
Synteny	MC11g1454

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGAAAATTAGGACAACAAATGATTTGAGGCCACAAGAATAATATATTAAAGGAACATGGGCTTGGGCTGAGAAGTTTCAGAAGTAAGCCCAACCCATAGAAGAAGCAGCAACAGCACTGCAGCAGTGAAGGCTGAAGCTCCGCGGCTGCCCTTCTCGTCCTTCCAGCGCAGGGAGCTGCCGAGTGTGAATGCAAGCCATGGAAGACATAGAAGACCTGCTGATCGGCGGCGGTGGAGGAGGCGGCGCTCCCCCTGGTTACCGGCTTCCGTTAACCGCAGTGGGTATAAAGCCGAAGAAGAAGAATAAGCTCAAATCCAATCCTGATGCCGATGATTCCAGCAACCAGCTCTCTCCGACCCAAAATCCACTTGTTCCTAAGATTCCAGGCACCCAGGTCTCTCTCTTCATGTCTCCTTATATCACGTAGAATCTATCTTCTCCGCTTCATTTTCCCGTTTCAATTGCGGATGCTAATAAGTTTTTGTTTGATATAATTTGGCAGACAATTTACGTTAAAACTTTTGGATGTTCTCACAACCAGGCAAGTCCTTCACCTCTACTTATTAAATTGATCACATATCGTCGTATTATTACCCTGACTTGATTGCAGGGCTAAGGCTTTAATTCTGTTCGAGTTTCTTTATTCAGATAATTGGTCTGAGGAACACTTAATCCAAATATAGGTCGATAAAAATGATTTTTAGTTTTTATGCTGGCAATTTGTGGAGTTCTGAATCTGGATTTTACTATTTTCATTTACGTTCCATCAATCTTTTTATCTTTTATGGGTTTTACCTAAGGCTTCCAATTGATGTAGATTCATTTGGAATTAAAGCTTTCAGTGCAATGTGGTAAATGTAAAACTACAACTTTTTTCGAGCAGACAGAGTGCGTAACACAGACATATCATATATTAAATCTATTATTTTTTTCTCTTTTCCTAATAAAGAACTTAAAAACTATCATTAATCAAACTGTGATTCAAAATTTCAAGAGGACAAGTCCAAATAACAAGGAGTGCCACAAGCTGAATGTAAAAAAGTTGGAATCACCATGTTATATTATTTTTTTGCGTAAGTAAGACACTCCACGCACACTGACTAGATGTTCTTCATAGAAAGCTCTTTGACAGCATTCAAAGCATCAAAGAAACAGCAGTGTCCAAATTGCACTCTTCCTGAACCAACTTTCTCTTCTTTCATCATGGTCTGCAAAAAAAATCTTAATGTGAGATATGTTTCACCTGTTTTGGACGAACCCAAGAAAATTTTTATTCCATAGAAGACTATACCCCATTTATTTGCAAAAACACACACATTTAGTAGATGATTCATGTCATGTTATCACTTTTACGTAGAATGCATCAGTCATCAGGGAGGTAAACGTAATAATGAGGTCTTCTCTAGCATTATTGGTGATTAATTTTAAATAATTCAATTAAATACATGGATCTTAGTGTGAATTCCATAAACCAAGTCAAATTATGAAATTAGGAGATTAGTATTTCGGATTGGAGAAGAAACTTACAAAATAATGATTCCCTAACCTGTGAACTGTCATTCATTATCTGTTTTTTTCTTTATCCTAAATCTCTTTTGGAGAAACTATCAAGTTTCTTCTTAGATATAGCAACTTTCTCAAATGTGAACAATGCGAAACACTGGACATTGCTTTTTAAAAAATCTGCTTAAGTGGCTATATATTTATTCTCTAATATCATTTCTTGAGTTGTAAATGTTTGGTGTTAATATGCCAATTTGTTTTCTATGAGTAATCTTAACAATACTCTAATATGTGAGGTTCTTTGTATTAACTGTGTTAGTTGTGCCATTATTGCTAAAAAATTGGATACAATTTTCTTCATTGTCTTCCTCATTGACAGAGCGACAGTGAATACATGGCTGGTCAGCTTTCAGCATTTGGTTATTTGTTAAGTGACAATCCTGAAGAGGCAGACTTGTGGCTTATAAATACGTATGATCTGTTCCTTCAAATTTTACTCTTCCTTATTTAGTCTGAATTTTATAGGAAATGTTCAATGAAAATTTCTTTCTTAGTTGCACAAAAGCATAAGTTGCCTGTGAATGCTTTCAAATGCTTCAAACTGTTCAGTAATCAAAGAATTTTATGTGCCTGTTACATGCATTATTACTTGGTTGTGGGCCTTAGCATACTATTCGCATCGTTCATTTAGTACATAGTTGTCTCTTCCAGGTTGGTAATTACATTACGTCTTCTTGAGGACATGAACCTTCAACTTCCTCTGCATAGTGTTAGCCCTGCGTATATGTGCTTTGGCTTAGATAGCATCCATATATGTGCTTTGGCACAGATCTATATTTTTGCTCATACTATCATGGATTTTTTCTGTCCATGTATAGAACATTCAAATTTGGAATTTTTTACTAATAGCCTGATAAATATTTATGCAGTTGCACAGTTAAATCCCCTAGCCAGTCTGCTATGGACACTCTAATAACAAAATGCAAGAATGCGAAAAAGCCTCTTGTGGTAGCTGGGTGTGTACCACAAGGTAGTAGAGATTTAAAGGAGCTAGAGGGGGTCAGTATAGTAGGAGTCCAGCAGATTGACCGTGTGGTAGAAGTTGTGGAAGAGACACTGAAAGGTCATGAAGTAAGGCTGTTAAATCGCAAGACACTGCCAGCGCTTGACCTCCCAAAAGTGCGAATTGATTCCCATTCTAGCTTTTTGAGTGTTCTATTTGAGTTTGATTTAATTCTTTAGCTTGTTTTTATTTTTTACCCTTTTGCTAACTTTTAAAAAATTTTAATTTAATTTTGAACAGGTAAGGAAGAACAAGTTCATTGAAATTCTTCCAATCAATGTTGGTTGTTTAGGTGCTTGCACTTATTGTAAGACAAAACATGCTCGTGGTCACTTGGGAAGTTACACTGTAGATAGCCTTGTAAGGACTTTATACTCATGTATGATTTTTGTTAAACGTATATCCTCCATTTTTTTAGTTAGAAAACTTTATGTTGTTTTTTATTAAAAAGATTTGCTAAAACTGTATTGCAGGTGAAGCGTGTAAGAACTGTCATCAACGAAGGAGTCAAGGAAGTATGGTTGAGCAGTGAAGATACTGGAGCGTATGGTATATTTACATTACTTCCATCTGAGATTTTTTTTCAATTTTGTAGTATATGACAAGCTGGTAGTTTCTTATACTTTATATAGTGCTGATTGAGTTATTTTATCAGAAGATGGTGTATTGTATCCACAGAAATATACAGAGTTGGAATTTCTAAAAATGTTGATGGAAAATGTTGCTATTATGACAATTATGGTCTAAACTATTGAAATTTGGAATGAAGATTATATTCTTCATCATGTGAACATCTAAGTTCTTCCAATTATCAATTTTTCTAATAGGTTACGTATTTAAAAAATAGTTTAAAAATTTTCAAAAATTACTAGCATCAAAATTGCAGAGCTTGAGAAATTTGATTCTTCTATCAAGTATGATAAGATTCCTTCCAAAAGCGTGAGAAGTTTTACTAGTCTTGTAGAGGAGTGTTTGGGACTCTTCTTGCGTTATTATGTTTTTCTTTTTCTTTCTTTCTTTCTTTATTTTTATTATGGGAAACCGAGCTTTCATGAAGAAACAAGAAAGAAAATACGCTTACAAAAAACCAGCCTTACAAAAGGAATCCAAACTAACAAATGTACTCCATTCCGAAAGAATAAAATATAATTGATAATTACAAAACAACTTAGTAACCAGAGCCCATAAAGAGATATTAACCTAACAATTTCCCACACCTCTTTGAAGGAAAGCTATAAATCTAGATACAATTAAAGTCATATCCAACCGTCAACCATCCATCTCAGGTCTTTATCTTGTCGGTTGGTTAGGTGGTCGCTTATAAGATTAGCAAAGAAATGGACAAGGTAGCCTGTGTAGGTTAAAAATAAACCAATGATTGGTTTGTATCATAACTTCTTCGGTAATTTTTTAGATGTTAGATGATAATTTTTTTCTGATTATATCAAAATTGAGCGTTGAAATAATGCATATTATCCTAGGAATATCAGGATTGTATAATATTTTTCCTTGCTAATGTCATATGATGAAATTTCAGGCCGTGACATTGGGGTCAATCTTCCAAGTTTATTAAATGCAATTGTTGCTGAGCTTCCACCGGATGCAAGCACAATGCTTCGGATTGGGATGACAAATCCTCCTTTTATTCTTGAGCACTTGAAAGAGATAGCTAATGTCTTGTGTCATCCATGCGTATACTCCTTTTTGCATGTGCCAGTTCAATCTGGAAGTGACGCAATCTTGAGTGTAAGTGTTGACTGTACCTAAATTTTCTTAGAATTTCTTTTGTAATTTCTTGATCCCTGTGCTGTTTTGGGGAATGAAAATGCTTATAATCCTAATGCTTGCTATTTCTCTCAGGCAATGAATCGAGAATATACAGTCGATGAATTCAGGACTGTAGTAGATACCTTAACTGAGCTTGTTCCTGGGATGCAGATTGCAACTGATATAATATGTGGATTTCCTGGTTAGTAAATGGGCTTAAGGCCACGTATTGATTCCTATACTTCTATTGTTGTCTCTCTGTGGGTGTGCATGGTTTCTTATTGCTTGCATGCCGTATTGGATTGGAAATACGAAATAACACTTAACATTAATCGATTTTATGTTGCAACTATAAATTATTGTTTCATCTTCAAAGGTGCAATATTTGAGCAATGAACTTACAAATATTTTAAAATATAGATATGTCTTAATTGTGAAACTTTATTGATTATTATGAAACCAATTGAAAAATTCGTTGTAGCCCTTGGTTGCAAAAAGTTCAAGCATGAATGATTTGTTTTAGCCTCTGTACAGCAGTGTGAAGATCATTTCTTTAAGGTTTGTCAGGACCTTTTTTCCCCTTTTGTTGCTTTGCACCCTCTTGAATCTTTGTTTCTCCTTCAACGATGCATATAGTGTAGATTCACATTTTGGGAAGCGTATAATATTTGGTAGTCCTAACCTTCATGACTAACAATTTTATTTTATGTAAATGATTGCCAAATTTATTGTCAATTATGGGGCTTTTCAGGGGAAACGGATGAAGATTTTTGTCAAACTATCAACCTTATTAAGGAGTACAAATTACCTCAAGTTCATATCTCACAGTTCTATCCTCGACCAGGTATGCAAATGACATCATGTTCAAGCCTCCTTATTTCATTGGATAATCCTACATAGACTTTAAAATAAGGGAACAATTTGACTTCAATGCTATTGCATGCTTAAGAATCGTTTGTCACAAAGGAGAAGGAACCTTGAGCTATTCCGTGTGTATTATTCAGGAGACATCACGGTCTTAAGATGGAGAGGTTTATTTTCCCAGGATATTGTTATACAATTGCTTAGGAAGCATAAATTTTTTTGTTTATTCAAACAGTTTATACATCTCTTCCCATTTAGTTTTCATATTTATTGCTTGCAACTTATTTAATGAAGTGAGGAAAAAGGTACATTCCTTTATTGATTTGTTTTTTCCCCTTTTTTTTCCTTCGTATAATTATCTTTTCAATGGAAAAACTAAGCTTCATTGAGAAAAATAGAAGTATGCAAAAGTGCCCACAAAAACAACAGCCTAGAAAGAGGGCCTGACAAAAACAAGATCTAACAAAACATAAAAGGGGTCCAATTAAAAATAATAATGCCTAAAGTTTCATTACAGAAATCTAGTTACGGATGCCCAAAGAGAGACATTGTACCAGGAATCAAGTTTCATTTAATTTAAAAGGTTTCCTGTTAGGATGGTATAAATTCCACATTTCTTTTTTCAATTTTAAAGAGCATTTGCTAATTATGGATTTCTCTATTATGCAAACAACATATTTTTTACTTAATTCAGATAAAGAAGTGTAATAATAGCCACAACAATGAATGAATTGTAGCAAGATTAATTATTCATGCATGTACACAATCTAGCAGCTAAAGAGTTTGCCTATGTTACCTCTGAAGAAACCCTGTCTGTAGCTGTATCTTCATTAAATTGATTGTCTAGGATATATCTGTCTAGACTATGCTTGATTTGTGTTTGGAACTTCTCATTTTGACCTTAAGTAAGGTGAATGTGATCATATGTTTTATATTTCCAACAGCCAAACATTTAAAATGAAAATACATTATCCTTCTATTGTCCTTCAGGGACACCTGCTGCTAGGATGAAGAAAGTTCCAAGTGCTATTGTCAAGAAACGAAGCCGTGAGTTGACTTCTGTTTTCGAAGCTTTTACACCGTATAATGGAATGGAGGGCAGAGTGGAAAGAATTTGGATAACAGAAATTGCAGCAGATGGCATTCACTTGGTAAGAGAGCTTCGCTGTCTAATGTCTATTTTTCCTAATTCTATCATGTTGTAATTTGCTGCAGTTTAGGGGTTGGTTTCAGGTGACTGCTATTACACGAATTATTGGACAATGGTAAAAAATTTGTTTCGATCAGCTTTGGGACAAAATTTGAGACATCTAGGAGGTTGTTTAAATAGGTCTTTTAGCTAGTGAATTGAAAGTGTGTCTACAGTGAAACAAGAACATAGGAAATAGAATAGACAACCCATTGCCACAGTTTGGGAATCAATATATAATTTTGGACTTGAGTCTGTTGTCTGCGTGCTTTAACAATATTTTTTTTTGTTAATATGCGTGAGTGTTTGGGCCAACTTGCGTGAACTTCGACTAATCTCACGAGACGACTGTTTGACCCTACGATATTTGGTTGCCAAGGTACCTCGTAGGATATTAAATCTTAGGTAGGTGGGCAGCTTGCTTTAACAAATTATCGTTAGAAAAAATCTACTAGATGGGTAATTAAAATTTAAACATTATAATTCAATGTGTTCGACTCAGTGTTTTCGAGGCGCAAGGCAGACTGCAAGGCGACGAGTCCTTTGTATCGCCTCGACACCCGATTGAAGCGAGGCGCACCTTAATGAATAAGTAAAAATTATATATATAGTGCATAAATTACCTTAAAAAAATCAATACCAAAATAGTAAATGTCAACAAGATGCATAAAACAACCAAATTCTATAAATTTAGTTCCGAATAAAGACAAGCAACATTAAGAAATTCTAGAGTCTGAAAATAGAACAAAAAGTAAATATAAGTAATCAAAGCTCATCATCCTCATCTAAATCTAAATTCTCTTTATCTTGGACGTCCTCACAATCTTCCTTTTCCAATTCTTCTTCTTCTTCGGTGTCATCATCATCATCACTAACCACAACTTGCACTGATTTTCGAAGTCTTGAAGTTGTAAGAGCGGGAGTAGCAAGCGACTTTCCTTTAGATCTTGCATATGTTCTAGTTTGCCTTATGGGCTCTCTAACACCACTGGTCTCGGCTACATCTCCCCATGTGAAGTCGTCATCATCAAAGACCAACTCATTTTCTTCTTGACCATCTTCTTCTTCGAGTGTTCCGATCAACCATTCATTACTTTCATCATTATGATCCAAAGTAATTGGATCAAGTTGGTCTTGTAGAGTGTAGCGCTCTTTAAGTGCTTGGTTATATTTTATATAAACCAAGTCATTAAGACGCTTTTGCTCTAACCTGTTCTTCTTCATGGAATGAATCTATAGAATTATTAACGATGTAGAGTCAAAAGTGGATTTTTCAATCTTCAACTAATAAGGGAAAGACCAAATTATTCTCACTTACGTGTTCAAAAACACTCCAATTGCGCTCACAGCCAGAAGCGTTGCACGTTAGACTAAAGACTCTCATGGTTAACTTTCGTAAAATCGGAGCATTAACACCATAAAGAGACCACCATGCAGCCGTGCACATATTAAACAAATGATTTAGAATTTTAGTAGTTCAAACTTAAATATGTCTATTAAAAATTTAAAACTAAAAGTAATAAATAATGTCATTACCTGGTGTTTTAACACCCCTCGTCCTTATTGCTACATTACTCGAAAACATGCCTTTTGCTTCATTGTACAATTCTAATTCGATTGAACATTGAACTTGATCATCGATAGATAGTATTAATCGTTCTATAACTTTGATCAGACCATCCATGACCTCAGGATCTTGCATTATTCTCTCTTTGTTGTCATAGAAAAGAGATGGATTCAAATAATATCCGGCTGCATGTAAAGGCCGATGAAGTTGACAATCCCACCTTTTATCAACAATCTCCCAAATCGGTCTATACTTCGCCTCATTTCCATTAAAACCACTCTTAATAGCCTCCTTTGCCCTATCCATAGCTTCGTAAATATAACCCATAGTCGGTTTATCACCATCTACTAGCCTCAACACACGAACTAAAGGGCCTGATGCTTTCAAAGTATACACAACTGAATTCCAAAAAGATGGCATCATAACGGTTTCAGCTGCTCTCTTCCCCTTTGGGTCCTTCGCCCATTTAGAGGTGGTCCATTTCTCAGAAATAAACATCTTCCTCAAATTGGCTTTATTTCTGCGAATACTGGCTAGGGTTAAAAATGAAGTTGCGAAGCGAGGCACTACTGGTCTTACCAATTCATGTTGGTTAGTAAATTCTCTCATCATTAGCACGTACAAGTGGTTGTAAATAAAACCACTAAGTGCCACAGCCTTCCTAAGACACCTTTTCACCTGCTCAATCTTTCCAATATCCTCCAAAATTAAATCCAGACAATGAGCAGCGCATGGTGTCCAATATAGATGAGGTCGCTTGGCTTCTAAAAGTTTTCCAGCTAGGACATAGTTTGAGGCATTATCCGTCACAACTTGGACAACGTTTTCTTCTCCAATCTTCTCCACCATTCCATCTAAGAGTTGAAAAATCTTTTCTCCAGTTTTGATGCATGAAGATGCATCGATGGATTCCATAAACAACGTTCCAAAAGATGAATGAACTAAAAAGTTGATTAGTGTCCTATCCCTCCTATCAGTCCACCCATCTGACATCAGCGAACAACCATTTCTCTTCCATTCTACCTCAGGATCGCCCATAAGATTTTTTATGTACTGTACTTCTTTTTTCAAGTACGTTACTCGAGCTTCATGATAAGGAGGAGGTATCAAACCAGGGCCAAATTGCCCCACAGCTTCTAGTATCACTTCAAAACTTCTCAAGCGAATAGTATTTAGAGGAATACCTGCTTGATATTTCCAACGCATGATGAATTGCACTGTAGCATCTCTCCCCTCTTTATCATATTTTTCAGTAATAGTTGATTGCTTCAACCTCTTCAATCGATTTCTATCATCAACAACTTTTCCAGGATGTTGATAAACAAATTTATCTATTGGCCCCTTTGGTCGTGGTGGTTTTAACACTGATGGACTACAAGAGTTACTACTTCCACTTCCACTTCCACTTCCTCTCCCTCTCCCACCCATACTACTCACATTATCACCTGTGTTTATTCTTGTTCGCTTGTTCATTGGGGTTGATGTTGATCCAGATCCACTACCACTACCACCAACTACTTCCATCTCATCTTCATCATCTTCTTCAATCAAAGTTAAATCTTCATACGTTGGGAGGGCATTACTCTCTTCCCTTCTTGCTTTTGTTCTTGTTTGATACTCCTTCAATTCTTCTCTCACATTAGTTGGGCATTTCAAACATGCCTCTACATTTCTATTTCCCCCAACCAAATGGTTTGTTGCTCGGCAAACTCCACCTTTGGTAATTTTTCCACAAAACATGCATTTGCAAGTGTTTGTATCTTTTGGATCATCCAATATGATGTATTGCCAACAGGGATGTTTGCATTTGTTTCCACCTTCTTCCATCCTAATATAATTCAAATTTGGAAGATTAAAAAAAAATGTAAAGGGAAGACTAAAGAGCCACTGTGAAGTGTGAAGAAAGCCAAAGATTTAGAAAACTTTTGTGTGGAATAAAAGAGAGGAAACGAAAAGGAGTAAATGAGATATAAGAAATAAAAAAAAAAAAAAAAAACCCTACGAAAGTCGGACTATCGGAGTGAGCGGCAACGGAGGCAGCGGGGGCGGCTGCGACGGTAGCAGACGACGATGGTGATGGTCGGAGACGGGGAAGCGGGCGACGGCCGGCGGAGACGACGGTGGTGAAGGCTGAAGATGAATGTCGAGAGGGAGGGGAGGCAGAAAAGGTTCATCGTTGTCGCACGCAGGGAAGGGTTTTTAAAAAGAAAAAACTTTAAAAATTAAAAAAAGAAAAAAAGACGCATTGCCTCCTTGAAGGAGGCGATCGCCTTGCCTCAGACGACTGGCGCGCCAAGGCGCCCAAAGGCGAGCGCCCGATTGGGCACCTTGCGAGGCGCACCTGGGCGAAAACGCCTCGCCTCTTGCCTGAGGCGAGCGCCCGAGTGGGCCTCGAAAACACTGGTTCCACTTAAGTTACAGGAATTGTGTTTGGACGGAGGAAAAAGCCCCAGCTCAACTGTAACCATCTTTTGGCCTTGAAGTGAATTATAATTAGATAACCTAACCAAAATTAGGATTTCAAGACACTAGAGGACTTCTTACATGGCTGTTTATATAACATATAAGAACTAGGCTGTATGGTCTTAGACTCTTAATTAATTCTAAGGGTTCTACAACACAAGGCATTTTCTTCAAACATGTCTACTTATAAGATATTGCTTATAATTGGCTTGTTTGTGAGTGAGTTAAATTTTATTCATTCTTTCATATATGTATATGAATTATACTAGTTATATTAGATTGGACCATACATAACCTTAGGTCTTGGCTATGAGGTGCCTGTCCTGAGTAGTGAGTATGCTCTACACTCTTAGACAACTTCTCCAATTTGCTAAACAGCATGATAGTATAACTATTCTCAGACTGTTAGAGAACTATCCAACTATTCTTCCATTTAGGCCTAGGATTTGTTTGTTTTCCTTGTCCTATTGGTGACTAGGAATGCAGGCTCAGAAGTTTGGCTTCTCCATTCTTCTATAAGTTTTCGTCTATTATTGTGTAAGTTGGCTGCTATGAAGGAAACTTTGAAACAGAATCTAGTTGAAGCAATTTGGAAAGGAAAGGGACCGAAAGGTGAAGGTTCTTATGTGCACATTAGCTTTTGAAGGACTGAACAGGGCTGAAAATTACAAAATAGGTGTCATGGGCCTATCTTATGAAATTCTTTTTGCAATAATCCCCCTATATCCTCTCCTTTCTGTTTTTCGTTCCTCTTTGAAAAATGGAACCATTGTTATTAAACTTTTTCTTAATCAATTCCCACATGCATTTTAGTTGCAACGGGAATGGAAGAGGGAGCTTCATTCTGTGATCCATATCTCATAAAACTGCTGTTTCTGTACTCTCTGATTCTAATGTTTCAAAGAACTGCTCTATCTTTCAATTAACCCGGTTTATTTGCTTCCCCAGGTTGGCCACACCAAGGGATACATACAGGTGCTCGTAATTGCCCCAGAAACCATGCTGGGAACTTCAGCCATGGTAAAGATAACATCCGTTGGAAGGTGGTCCGTTTTTGGGGACGTGATCGAAACCATCAGCACCAGACATCATAAAACAACTACTCTCGAAGAGATGCCGATCCAAGACAAAGTTCCTTGCTGTAACACCCACGAGAAGTGTTCAAGTTCCACAGAACCCGAACCCTGTGCTTGTGGGCCAGAAAGCTGCAAAGCACCAGTAACTCTGGATAATAAAGATGATTCTTCAAGTAATATTTCATTGGCTGAAGAGCCAAAACGGAAAAATGTGATAGAATGGCTACTGAGGAGACGGAAGAGGCATGTGGCCAAGAGAGAGGAGAGAGAAAGTAGTGGCATAGCATCTGAGAGAAAGCAGGCATCGGGTGAGTGGGGCTTTGTTGATAGGATTCTTGTTGGTGGAATACTAATCAGTATCTTTGCAATTGTTGGTGTACTGTTTCATCTTGGATCCACCACTCTTCTCCTACTCTAATTGATGATATTATACGATGCCAATTTGTATGGGACCATTTTGAAGAAAGTTTGTAACAAGAGTTTCTATTTCCTCGCTCCTTTCCCAATTCCTTTTTCAGCTTTCTTTTGTACAAGTAGAATTTTTGGGTAGTCGAAATTGCGAGTGTAATTTAATTTGAGATTAGTGATTTTCTTTTCCAACA

mRNA sequence

GAGAAAATTAGGACAACAAATGATTTGAGGCCACAAGAATAATATATTAAAGGAACATGGGCTTGGGCTGAGAAGTTTCAGAAGTAAGCCCAACCCATAGAAGAAGCAGCAACAGCACTGCAGCAGTGAAGGCTGAAGCTCCGCGGCTGCCCTTCTCGTCCTTCCAGCGCAGGGAGCTGCCGAGTGTGAATGCAAGCCATGGAAGACATAGAAGACCTGCTGATCGGCGGCGGTGGAGGAGGCGGCGCTCCCCCTGGTTACCGGCTTCCGTTAACCGCAGTGGGTATAAAGCCGAAGAAGAAGAATAAGCTCAAATCCAATCCTGATGCCGATGATTCCAGCAACCAGCTCTCTCCGACCCAAAATCCACTTGTTCCTAAGATTCCAGGCACCCAGACAATTTACGTTAAAACTTTTGGATGTTCTCACAACCAGGCAAGGAGTGAATACATGGCTGGTCAGCTTTCAGCATTTGGTTATTTGTTAAGTGACAATCCTGAAGAGGCAGACTTGTGGCTTATAAATACTTGCACAGTTAAATCCCCTAGCCAGTCTGCTATGGACACTCTAATAACAAAATGCAAGAATGCGAAAAAGCCTCTTGTGGTAGCTGGGTGTGTACCACAAGGTAGTAGAGATTTAAAGGAGCTAGAGGGGGTCAGTATAGTAGGAGTCCAGCAGATTGACCGTGTGGTAGAAGTTGTGGAAGAGACACTGAAAGGTCATGAAGTAAGGAAGAACAAGTTCATTGAAATTCTTCCAATCAATGTTGGTTGTTTAGGTGCTTGCACTTATTGTAAGACAAAACATGCTCGTGGTCACTTGGGAAGTTACACTGTAGATAGCCTTGTGAAGCGTGTAAGAACTGTCATCAACGAAGGAGTCAAGGAAGTATGGTTGAGCAGTGAAGATACTGGAGCGTATGGCCGTGACATTGGGGTCAATCTTCCAAGTTTATTAAATGCAATTGTTGCTGAGCTTCCACCGGATGCAAGCACAATGCTTCGGATTGGGATGACAAATCCTCCTTTTATTCTTGAGCACTTGAAAGAGATAGCTAATGTCTTGTGTCATCCATGCGTATACTCCTTTTTGCATGTGCCAGTTCAATCTGGAAGTGACGCAATCTTGAGTGCAATGAATCGAGAATATACAGTCGATGAATTCAGGACTGTAGTAGATACCTTAACTGAGCTTGTTCCTGGGATGCAGATTGCAACTGATATAATATGTGGATTTCCTGGGGAAACGGATGAAGATTTTTGTCAAACTATCAACCTTATTAAGGAGTACAAATTACCTCAAGTTCATATCTCACAGTTCTATCCTCGACCAGGGACACCTGCTGCTAGGATGAAGAAAGTTCCAAGTGCTATTGTCAAGAAACGAAGCCGTGAGTTGACTTCTGTTTTCGAAGCTTTTACACCGTATAATGGAATGGAGGGCAGAGTGGAAAGAATTTGGATAACAGAAATTGCAGCAGATGGCATTCACTTGGTTGGCCACACCAAGGGATACATACAGGTGCTCGTAATTGCCCCAGAAACCATGCTGGGAACTTCAGCCATGGTAAAGATAACATCCGTTGGAAGGTGGTCCGTTTTTGGGGACGTGATCGAAACCATCAGCACCAGACATCATAAAACAACTACTCTCGAAGAGATGCCGATCCAAGACAAAGTTCCTTGCTGTAACACCCACGAGAAGTGTTCAAGTTCCACAGAACCCGAACCCTGTGCTTGTGGGCCAGAAAGCTGCAAAGCACCAGTAACTCTGGATAATAAAGATGATTCTTCAAGTAATATTTCATTGGCTGAAGAGCCAAAACGGAAAAATGTGATAGAATGGCTACTGAGGAGACGGAAGAGGCATGTGGCCAAGAGAGAGGAGAGAGAAAGTAGTGGCATAGCATCTGAGAGAAAGCAGGCATCGGGTGAGTGGGGCTTTGTTGATAGGATTCTTGTTGGTGGAATACTAATCAGTATCTTTGCAATTGTTGGTGTACTGTTTCATCTTGGATCCACCACTCTTCTCCTACTCTAATTGATGATATTATACGATGCCAATTTGTATGGGACCATTTTGAAGAAAGTTTGTAACAAGAGTTTCTATTTCCTCGCTCCTTTCCCAATTCCTTTTTCAGCTTTCTTTTGTACAAGTAGAATTTTTGGGTAGTCGAAATTGCGAGTGTAATTTAATTTGAGATTAGTGATTTTCTTTTCCAACA

Coding sequence (CDS)

ATGCAAGCCATGGAAGACATAGAAGACCTGCTGATCGGCGGCGGTGGAGGAGGCGGCGCTCCCCCTGGTTACCGGCTTCCGTTAACCGCAGTGGGTATAAAGCCGAAGAAGAAGAATAAGCTCAAATCCAATCCTGATGCCGATGATTCCAGCAACCAGCTCTCTCCGACCCAAAATCCACTTGTTCCTAAGATTCCAGGCACCCAGACAATTTACGTTAAAACTTTTGGATGTTCTCACAACCAGGCAAGGAGTGAATACATGGCTGGTCAGCTTTCAGCATTTGGTTATTTGTTAAGTGACAATCCTGAAGAGGCAGACTTGTGGCTTATAAATACTTGCACAGTTAAATCCCCTAGCCAGTCTGCTATGGACACTCTAATAACAAAATGCAAGAATGCGAAAAAGCCTCTTGTGGTAGCTGGGTGTGTACCACAAGGTAGTAGAGATTTAAAGGAGCTAGAGGGGGTCAGTATAGTAGGAGTCCAGCAGATTGACCGTGTGGTAGAAGTTGTGGAAGAGACACTGAAAGGTCATGAAGTAAGGAAGAACAAGTTCATTGAAATTCTTCCAATCAATGTTGGTTGTTTAGGTGCTTGCACTTATTGTAAGACAAAACATGCTCGTGGTCACTTGGGAAGTTACACTGTAGATAGCCTTGTGAAGCGTGTAAGAACTGTCATCAACGAAGGAGTCAAGGAAGTATGGTTGAGCAGTGAAGATACTGGAGCGTATGGCCGTGACATTGGGGTCAATCTTCCAAGTTTATTAAATGCAATTGTTGCTGAGCTTCCACCGGATGCAAGCACAATGCTTCGGATTGGGATGACAAATCCTCCTTTTATTCTTGAGCACTTGAAAGAGATAGCTAATGTCTTGTGTCATCCATGCGTATACTCCTTTTTGCATGTGCCAGTTCAATCTGGAAGTGACGCAATCTTGAGTGCAATGAATCGAGAATATACAGTCGATGAATTCAGGACTGTAGTAGATACCTTAACTGAGCTTGTTCCTGGGATGCAGATTGCAACTGATATAATATGTGGATTTCCTGGGGAAACGGATGAAGATTTTTGTCAAACTATCAACCTTATTAAGGAGTACAAATTACCTCAAGTTCATATCTCACAGTTCTATCCTCGACCAGGGACACCTGCTGCTAGGATGAAGAAAGTTCCAAGTGCTATTGTCAAGAAACGAAGCCGTGAGTTGACTTCTGTTTTCGAAGCTTTTACACCGTATAATGGAATGGAGGGCAGAGTGGAAAGAATTTGGATAACAGAAATTGCAGCAGATGGCATTCACTTGGTTGGCCACACCAAGGGATACATACAGGTGCTCGTAATTGCCCCAGAAACCATGCTGGGAACTTCAGCCATGGTAAAGATAACATCCGTTGGAAGGTGGTCCGTTTTTGGGGACGTGATCGAAACCATCAGCACCAGACATCATAAAACAACTACTCTCGAAGAGATGCCGATCCAAGACAAAGTTCCTTGCTGTAACACCCACGAGAAGTGTTCAAGTTCCACAGAACCCGAACCCTGTGCTTGTGGGCCAGAAAGCTGCAAAGCACCAGTAACTCTGGATAATAAAGATGATTCTTCAAGTAATATTTCATTGGCTGAAGAGCCAAAACGGAAAAATGTGATAGAATGGCTACTGAGGAGACGGAAGAGGCATGTGGCCAAGAGAGAGGAGAGAGAAAGTAGTGGCATAGCATCTGAGAGAAAGCAGGCATCGGGTGAGTGGGGCTTTGTTGATAGGATTCTTGTTGGTGGAATACTAATCAGTATCTTTGCAATTGTTGGTGTACTGTTTCATCTTGGATCCACCACTCTTCTCCTACTCTAA

Protein sequence

MQAMEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVPKIPGTQTIYVKTFGCSHNQARSEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIETISTRHHKTTTLEEMPIQDKVPCCNTHEKCSSSTEPEPCACGPESCKAPVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQASGEWGFVDRILVGGILISIFAIVGVLFHLGSTTLLLL

Homology

[Close]

BLAST of MC11g1454 vs. ExPASy Swiss-Prot
Match: Q291H5 (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=GA19679 PE=3 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 1.6e-133
Identity = 250/499 (50.10%), Postives = 333/499 (66.73%), Query Frame = 0


Query: 4   MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVP 63
           ++DIEDL+         P        +V ++ KK+ ++K   DA++      PT  P + 
Sbjct: 13  IDDIEDLI---SAEDVKPQERYQNKKSVTVRAKKRVQIKPETDAEE-----KPTPRPTIY 72

Query: 64  K--IPGTQTIYVKTFGCSHNQARSEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPSQ 123
           +  IPGTQ ++VKT+GC+HN + SEYMAGQL+A+GY LS   +EADLWL+N+CTVK+PS+
Sbjct: 73  ESVIPGTQKVFVKTWGCAHNNSDSEYMAGQLAAYGYKLS-GKDEADLWLLNSCTVKNPSE 132

Query: 124 SAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGH-- 183
                 I    +  K +VVAGCVPQG+     L G+S++GVQQIDRVVEVVEETLKGH  
Sbjct: 133 DTFRNEIESGMSNGKHIVVAGCVPQGAPKSDYLRGLSVIGVQQIDRVVEVVEETLKGHSV 192

Query: 184 ---------------------EVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVD 243
                                +VRKN  IEI+ IN GCL  CTYCKTKHARG L SY  +
Sbjct: 193 RLLQNKKVHGRRVAGAPLSLPKVRKNPLIEIISINTGCLNQCTYCKTKHARGDLASYPPE 252

Query: 244 SLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTN 303
            +V R R    EG  E+WL+SEDTGAYGRDIG +LP LL  +V  +P     MLR+GMTN
Sbjct: 253 EIVDRARQSFAEGCCEIWLTSEDTGAYGRDIGSSLPELLWKLVEVIPEHC--MLRVGMTN 312

Query: 304 PPFILEHLKEIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVP 363
           PP+ILEHL+E+A VL HP VY+FLHVPVQSGSD++L  M REY   +F  VVD L E VP
Sbjct: 313 PPYILEHLEEVAKVLQHPRVYAFLHVPVQSGSDSVLGEMKREYCRKDFEHVVDFLRERVP 372

Query: 364 GMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVK 423
           G+ IATDIICGFP ET+EDF +T+ L   Y+ P + I+QF+PRPGTPAA+M+++P+ +VK
Sbjct: 373 GVTIATDIICGFPTETEEDFEETMTLCGRYRFPSLFINQFFPRPGTPAAKMERIPANLVK 432

Query: 424 KRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTS 478
           KR++ LT +F ++ PY    G +  + +TEI+ D +H VGH K Y QVL+   + +LGT 
Sbjct: 433 KRTKRLTDLFYSYEPYAQRVGEMYTVLVTEISHDKLHYVGHNKSYEQVLLPMRDNLLGTR 492

[Close]

BLAST of MC11g1454 vs. ExPASy Swiss-Prot
Match: Q7K4W1 (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Drosophila melanogaster OX=7227 GN=CG6550 PE=2 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 7.9e-133
Identity = 254/521 (48.75%), Postives = 341/521 (65.45%), Query Frame = 0


Query: 4   MEDIEDLLIGGGGGGGAPPGYRLP-LTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLV 63
           ++DIEDL+          P  R      V ++ KK+++++   ++ +   +  PT +  V
Sbjct: 13  VDDIEDLI----SADDVKPRERYENKKTVTVRAKKRSQIRL--ESQEEEEKPKPTIHESV 72

Query: 64  PKIPGTQTIYVKTFGCSHNQARSEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPSQS 123
             IPGTQ ++VKT+GC+HN + SEYMAGQL+A+GY LS   EEADLWL+N+CTVK+PS+ 
Sbjct: 73  --IPGTQKVFVKTWGCAHNNSDSEYMAGQLAAYGYRLS-GKEEADLWLLNSCTVKNPSED 132

Query: 124 AMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGH--- 183
                I       K +VVAGCVPQG+     L G+S++GVQQIDRVVEVVEETLKGH   
Sbjct: 133 TFRNEIESGMRNGKHVVVAGCVPQGAPKSDYLNGLSVIGVQQIDRVVEVVEETLKGHSVQ 192

Query: 184 ---------------------EVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVD 243
                                +VRKN  IEI+ IN GCL  CTYCKTKHARG L SY  +
Sbjct: 193 LLQNKKKVHGRRVAGAPLSLPKVRKNPLIEIISINSGCLNQCTYCKTKHARGDLASYPPE 252

Query: 244 SLVKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTN 303
            +V+R R    EG  E+WL+SEDTGAYGRDIG +LP LL  +V  +P     MLR+GMTN
Sbjct: 253 EVVERARQSFAEGCCEIWLTSEDTGAYGRDIGSSLPELLWQLVEVIPEHC--MLRVGMTN 312

Query: 304 PPFILEHLKEIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVP 363
           PP+ILEHL+E+ANVL HP VYSFLHVPVQSGSD++L  M REY   +F  VVD L E VP
Sbjct: 313 PPYILEHLEEVANVLQHPRVYSFLHVPVQSGSDSVLGEMKREYCRQDFEHVVDFLRERVP 372

Query: 364 GMQIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVK 423
           G+ IATDIICGFP ET++DF +T+ L  +Y+ P + I+QF+PRPGTPAA+M ++P+ +VK
Sbjct: 373 GVTIATDIICGFPTETEDDFEETMTLCAKYRFPSLFINQFFPRPGTPAAKMDRIPANLVK 432

Query: 424 KRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTS 483
           KR++ LT +F ++ PY    G +  + +TE++ D +H VGH K Y QVL+   + +LGT 
Sbjct: 433 KRTKRLTDLFYSYEPYADRVGEIYTVLVTEVSHDKLHYVGHNKSYEQVLLPMRDNLLGTR 492

Query: 484 AMVKITSVGRWSVFGDVI--ETISTRHHKTTTLEEMPIQDK 498
             V+ITS  ++S+ G+++  E   TR  K   L  + +Q +
Sbjct: 493 VHVRITSASKFSMVGEILDDERDWTRCAKNQELPNVQVQTR 522

[Close]

BLAST of MC11g1454 vs. ExPASy Swiss-Prot
Match: Q6P4Y0 (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Xenopus tropicalis OX=8364 GN=cdkal1 PE=2 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 6.7e-132
Identity = 236/476 (49.58%), Postives = 323/476 (67.86%), Query Frame = 0


Query: 28  LTAVGIKPKKKNKLKSN--PDA-DDSSNQLSPTQNPLVPKIPGTQTIYVKTFGCSHNQAR 87
           ++A   KP  +   + N  P A   + N++   + P    IPGTQ I+++T+GCSHN + 
Sbjct: 16  VSATDPKPHDRQNARQNIVPRARKRNKNKIQEEEPPADSTIPGTQKIWIRTWGCSHNNSD 75

Query: 88  SEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCV 147
            EYMAGQL+A+GY +++ PE+ADLWL+N+CTVKSP++      I K + A K +V++GCV
Sbjct: 76  GEYMAGQLAAYGYSITEQPEQADLWLLNSCTVKSPAEDHFRNSIKKAQEANKKVVLSGCV 135

Query: 148 PQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGH-----------------------EV 207
           PQ     + ++G+SI+GVQQIDRVVEVVEET+KGH                       ++
Sbjct: 136 PQAQPRQEYMKGLSIIGVQQIDRVVEVVEETIKGHSVRLLGQKKDNGKRLGGARLDLPKI 195

Query: 208 RKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSED 267
           RKN  IEI+ IN GCL ACTYCKTKHARG L SY V+ LV R      EGV E+WL+SED
Sbjct: 196 RKNPLIEIISINTGCLNACTYCKTKHARGELASYPVEELVDRAAQSFQEGVCEIWLTSED 255

Query: 268 TGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIANVLCHPCVYSF 327
           TGAYGRDIG +LP+LL  +V  +P  A  MLR+GMTNPP+ILEHL+E+A +L HP VY+F
Sbjct: 256 TGAYGRDIGTDLPTLLWKLVEVIPEGA--MLRLGMTNPPYILEHLEEMAKILNHPRVYAF 315

Query: 328 LHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQT 387
           LH+PVQS SD++L  M REY + +F+ VVD L E VPG+ IATDIICGFPGETDEDF +T
Sbjct: 316 LHIPVQSASDSVLMDMKREYCIADFKRVVDFLKERVPGITIATDIICGFPGETDEDFKET 375

Query: 388 INLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRV 447
           + L++EYK P + I+QFYPRPGTPAA+M++VP+ + K+R++EL+ +F +++PY+   G  
Sbjct: 376 LKLVEEYKFPSLFINQFYPRPGTPAAKMEQVPAHVKKQRTKELSQLFHSYSPYDHKIGEE 435

Query: 448 ERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIE 478
           + + +TE + D  + V H + Y QVLV      +G    VKI   G+  + G  ++
Sbjct: 436 QHVLVTEESFDSQYYVSHNRFYEQVLVPKDPAFVGKMVEVKIFEAGKHFMKGQPVQ 489

[Close]

BLAST of MC11g1454 vs. ExPASy Swiss-Prot
Match: Q6NS26 (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Xenopus laevis OX=8355 GN=cdkal1 PE=2 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 9.7e-131
Identity = 236/476 (49.58%), Postives = 321/476 (67.44%), Query Frame = 0


Query: 28  LTAVGIKPKKKNKLKSN--PDA-DDSSNQLSPTQNPLVPKIPGTQTIYVKTFGCSHNQAR 87
           ++A   KP  +   + N  P A   + N +   + P    IPGTQ I+++T+GCSHN + 
Sbjct: 16  VSATDPKPHDRQSARKNIVPRARKRNKNNIQEEEPPADSTIPGTQKIWIRTWGCSHNNSD 75

Query: 88  SEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCV 147
            EYMAGQL+A+GY +++ PE+ADLWL+N+CTVKSP++      I K + A K +VV+GCV
Sbjct: 76  GEYMAGQLAAYGYSITEQPEKADLWLLNSCTVKSPAEDHFRNSIKKAQEANKKVVVSGCV 135

Query: 148 PQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGH-----------------------EV 207
           PQ       ++G+SI+GVQQIDRVVEVVEET+KGH                       ++
Sbjct: 136 PQAQPRQDYMKGLSIIGVQQIDRVVEVVEETIKGHSVRLLGQKKDNGKRLGGARLDLPKI 195

Query: 208 RKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSED 267
           RKN  IEI+ IN GCL ACTYCKTKHARG L SY V+ LV R      EGV E+WL+SED
Sbjct: 196 RKNPLIEIISINTGCLNACTYCKTKHARGELASYPVEELVDRATQSFQEGVCEIWLTSED 255

Query: 268 TGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIANVLCHPCVYSF 327
           TGAYGRDIG +LP+LL  +V  +P  A  MLR+GMTNPP+ILEHL+E+A +L HP VY+F
Sbjct: 256 TGAYGRDIGTDLPTLLWKLVEVIPEGA--MLRLGMTNPPYILEHLEEMAKILNHPRVYAF 315

Query: 328 LHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFCQT 387
           LH+PVQS SD++L  M REY + +F+ VVD L E VPG+ IATDIICGFPGETDEDF +T
Sbjct: 316 LHIPVQSASDSVLMDMKREYCIADFKRVVDFLKERVPGITIATDIICGFPGETDEDFKET 375

Query: 388 INLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRV 447
           + L++EYK P + I+QFYPRPGTPAA+M++V + + K+R++EL+ +F ++ PY+   G+ 
Sbjct: 376 LKLVEEYKFPSLFINQFYPRPGTPAAKMEQVLAHVKKRRTKELSQLFHSYDPYDHKIGQK 435

Query: 448 ERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVGRWSVFGDVIE 478
           +++ +TE + D  + V H + Y QVLV      +G    VKI   G+  + G  ++
Sbjct: 436 QQVLVTEESFDSQYYVAHNRFYEQVLVPKDPDFMGKMVEVKIFEAGKHFMKGQPVQ 489

[Close]

BLAST of MC11g1454 vs. ExPASy Swiss-Prot
Match: Q5VV42 (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Homo sapiens OX=9606 GN=CDKAL1 PE=1 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 1.2e-128
Identity = 237/496 (47.78%), Postives = 324/496 (65.32%), Query Frame = 0


Query: 4   MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVP 63
           ++DIED++         P         V  K +++N  K   + ++S         P   
Sbjct: 10  LDDIEDIV---SQEDSKPQDRHFVRKDVVPKVRRRNTQKYLQEEENSP--------PSDS 69

Query: 64  KIPGTQTIYVKTFGCSHNQARSEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPSQSA 123
            IPG Q I+++T+GCSHN +  EYMAGQL+A+GY +++N  +ADLWL+N+CTVK+P++  
Sbjct: 70  TIPGIQKIWIRTWGCSHNNSDGEYMAGQLAAYGYKITENASDADLWLLNSCTVKNPAEDH 129

Query: 124 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGH---- 183
               I K +   K +V+AGCVPQ       L+G+SI+GVQQIDRVVEVVEET+KGH    
Sbjct: 130 FRNSIKKAQEENKKIVLAGCVPQAQPRQDYLKGLSIIGVQQIDRVVEVVEETIKGHSVRL 189

Query: 184 -------------------EVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSL 243
                              ++RKN  IEI+ IN GCL ACTYCKTKHARG+L SY +D L
Sbjct: 190 LGQKKDNGRRLGGARLDLPKIRKNPLIEIISINTGCLNACTYCKTKHARGNLASYPIDEL 249

Query: 244 VKRVRTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPP 303
           V R +    EGV E+WL+SEDTGAYGRDIG NLP+LL  +V  +P  A  MLR+GMTNPP
Sbjct: 250 VDRAKQSFQEGVCEIWLTSEDTGAYGRDIGTNLPTLLWKLVEVIPEGA--MLRLGMTNPP 309

Query: 304 FILEHLKEIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGM 363
           +ILEHL+E+A +L HP VY+FLH+PVQS SD++L  M REY V +F+ VVD L E VPG+
Sbjct: 310 YILEHLEEMAKILNHPRVYAFLHIPVQSASDSVLMEMKREYCVADFKRVVDFLKEKVPGI 369

Query: 364 QIATDIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKR 423
            IATDIICGFPGETD+DF +T+ L++EYK P + I+QFYPRPGTPAA+M++VP+ + K+R
Sbjct: 370 TIATDIICGFPGETDQDFQETVKLVEEYKFPSLFINQFYPRPGTPAAKMEQVPAQVKKQR 429

Query: 424 SRELTSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAM 477
           +++L+ VF +++PY+   G  +++ +TE + D    V H + Y QVLV      +G    
Sbjct: 430 TKDLSRVFHSYSPYDHKIGERQQVLVTEESFDSKFYVAHNQFYEQVLVPKNPAFMGKMVE 489

[Close]

BLAST of MC11g1454 vs. NCBI nr
Match: XP_022155234.1 (threonylcarbamoyladenosine tRNA methylthiotransferase [Momordica charantia])

HSP 1 Score: 1212 bits (3135), Expect = 0.0
Identity = 612/633 (96.68%), Postives = 615/633 (97.16%), Query Frame = 0


Query: 1   MQAMEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNP 60
           MQAMEDIEDLLIGGGGGGGAPPGYRLPLTAVG+KPKKKNKLKSNPDADDSSNQLSPTQNP
Sbjct: 1   MQAMEDIEDLLIGGGGGGGAPPGYRLPLTAVGLKPKKKNKLKSNPDADDSSNQLSPTQNP 60

Query: 61  LVPKIPGTQTIYVKTFGCSHNQARSEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPS 120
           LVPKIPGTQTIYVKTFGCSHNQ+ SEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPS
Sbjct: 61  LVPKIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPS 120

Query: 121 QSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE 180
           QSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE
Sbjct: 121 QSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE 180

Query: 181 VR----------------KNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRV 240
           VR                KNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRV
Sbjct: 181 VRLLNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRV 240

Query: 241 RTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILE 300
           RTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILE
Sbjct: 241 RTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILE 300

Query: 301 HLKEIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIAT 360
           HLKEIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIAT
Sbjct: 301 HLKEIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIAT 360

Query: 361 DIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSREL 420
           DIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSREL
Sbjct: 361 DIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSREL 420

Query: 421 TSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKIT 480
           TSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVK+T
Sbjct: 421 TSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKVT 480

Query: 481 SVGRWSVFGDVIETISTRHHKTTTLEEMPIQDKVPCCNTHEKCSSSTEPEPCACGPESCK 540
           SVGRWSVFGDVIETISTRHHKTTTLEEMPIQDKVPCCNTHEKCSSSTEPEPCACGPESCK
Sbjct: 481 SVGRWSVFGDVIETISTRHHKTTTLEEMPIQDKVPCCNTHEKCSSSTEPEPCACGPESCK 540

Query: 541 APVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQASGEW 600
           APVTLDNKDDSSSNISLAEEPKRKNVIEWL RRRKRHVAKREERESSGIASERKQASGEW
Sbjct: 541 APVTLDNKDDSSSNISLAEEPKRKNVIEWLPRRRKRHVAKREERESSGIASERKQASGEW 600

Query: 601 GFVDRILVGGILISIFAIVGVLFHLGSTTLLLL 617
           GFVDRILVGGILISIFAIVGVLFHLGSTTLLLL
Sbjct: 601 GFVDRILVGGILISIFAIVGVLFHLGSTTLLLL 633

[Close]

BLAST of MC11g1454 vs. NCBI nr
Match: XP_022949069.1 (threonylcarbamoyladenosine tRNA methylthiotransferase [Cucurbita moschata])

HSP 1 Score: 1083 bits (2802), Expect = 0.0
Identity = 554/634 (87.38%), Postives = 575/634 (90.69%), Query Frame = 0


Query: 4   MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVP 63
           MEDIEDLLIGGGGGG APPGYRLP++AVG+KPKKKNKLKSNPDADDSS+ L PTQN L P
Sbjct: 1   MEDIEDLLIGGGGGG-APPGYRLPISAVGVKPKKKNKLKSNPDADDSSSGLFPTQNLLAP 60

Query: 64  KIPGTQTIYVKTFGCSHNQARSEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPSQSA 123
           KIPGTQTIYVKTFGCSHNQ+ SEYMAGQLSAFGYLLS+NPEEADLWLINTCTVKSPSQSA
Sbjct: 61  KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSENPEEADLWLINTCTVKSPSQSA 120

Query: 124 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVR- 183
           MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVR 
Sbjct: 121 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 180

Query: 184 ---------------KNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTV 243
                          KNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV RVRTV
Sbjct: 181 LNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVNRVRTV 240

Query: 244 INEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK 303
           INEGV+E+WLSSEDTGAYGRDIGVNLP LLNAIVAELPPDASTMLRIGMTNPPFILEHLK
Sbjct: 241 INEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLK 300

Query: 304 EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDII 363
           EIANVLCHPCVYSFLHVPVQSGSD ILSAMNREYTV EFRTVVDTLTELVPGMQ+ATDII
Sbjct: 301 EIANVLCHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDII 360

Query: 364 CGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 423
           CGFPGETDEDFC+TINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV
Sbjct: 361 CGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 420

Query: 424 FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVG 483
           FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVI  ETMLGTSA+VKITSVG
Sbjct: 421 FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIGHETMLGTSALVKITSVG 480

Query: 484 RWSVFGDVIETISTRHHKTTTLEEMPIQDKVP-CCNTHEKCSSSTEPEPCACGPESC-KA 543
           RWSVFG+VIETI T+HHKTT LE+MPIQ KVP C + HE C+ S EPE CACGPESC K 
Sbjct: 481 RWSVFGEVIETIVTKHHKTTALEDMPIQAKVPPCSDAHETCACSVEPEACACGPESCCKG 540

Query: 544 PVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQASG--- 603
           PV LD KDDSS NI L EEPKRKN++EWLLR+RK H  KREERES  + SERKQAS    
Sbjct: 541 PVALDEKDDSSRNIPLPEEPKRKNLVEWLLRKRKSHAPKREERESP-VVSERKQASAGGS 600

Query: 604 --EWGFVDRILVGGILISIFAIVGVLFHLGSTTL 614
             EWG VDRILVGGILISIF IVG+LFHLGSTTL
Sbjct: 601 MDEWGVVDRILVGGILISIFTIVGLLFHLGSTTL 632

[Close]

BLAST of MC11g1454 vs. NCBI nr
Match: XP_022997808.1 (threonylcarbamoyladenosine tRNA methylthiotransferase [Cucurbita maxima])

HSP 1 Score: 1082 bits (2797), Expect = 0.0
Identity = 552/634 (87.07%), Postives = 573/634 (90.38%), Query Frame = 0


Query: 4   MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVP 63
           MEDIEDLLIGGGGGGGAPPGYRLP++AVG+K KKKNKLK NPDADDSS+ L PTQN L P
Sbjct: 1   MEDIEDLLIGGGGGGGAPPGYRLPISAVGVKSKKKNKLKPNPDADDSSSGLFPTQNLLAP 60

Query: 64  KIPGTQTIYVKTFGCSHNQARSEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPSQSA 123
           KIPGTQTIYVKTFGCSHNQ+ SEYMAGQLSAFGYLLS+NPEEADLWLINTCTVKSPSQSA
Sbjct: 61  KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSENPEEADLWLINTCTVKSPSQSA 120

Query: 124 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVR- 183
           MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVR 
Sbjct: 121 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 180

Query: 184 ---------------KNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTV 243
                          KNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV RVRTV
Sbjct: 181 LNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVNRVRTV 240

Query: 244 INEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK 303
           INEGV+E+WLSSEDTGAYGRDIGVNLP LLNAIVAELPPDASTMLRIGMTNPPFILEHLK
Sbjct: 241 INEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLK 300

Query: 304 EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDII 363
           EIANVLCHPCVYSFLHVPVQSGSD ILSAMNREYTV EFRTVVDTLTELVPGMQ+ATDII
Sbjct: 301 EIANVLCHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDII 360

Query: 364 CGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 423
           CGFPGETDEDFC+TINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV
Sbjct: 361 CGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 420

Query: 424 FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVG 483
           FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVI  E MLGTSA+VKITSVG
Sbjct: 421 FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIGHEAMLGTSALVKITSVG 480

Query: 484 RWSVFGDVIETISTRHHKTTTLEEMPIQDKVP-CCNTHEKCSSSTEPEPCACGPESC-KA 543
           RWSVFG+VIETI T+HHKTT LE+MPIQ KVP C N+HE C+ S EPE CACGPESC K 
Sbjct: 481 RWSVFGEVIETIVTKHHKTTALEDMPIQAKVPPCSNSHETCACSVEPEACACGPESCCKG 540

Query: 544 PVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQASG--- 603
           PV LD KDDSS NI L EEPKRKN++EWLLR+RK HV KREE ES  + S RKQAS    
Sbjct: 541 PVALDEKDDSSRNIPLPEEPKRKNLVEWLLRKRKSHVPKREEGESP-VVSGRKQASAGGS 600

Query: 604 --EWGFVDRILVGGILISIFAIVGVLFHLGSTTL 614
             EWG VDRILVGGILISIF IVG+LFHLGSTTL
Sbjct: 601 MSEWGVVDRILVGGILISIFTIVGLLFHLGSTTL 633

[Close]

BLAST of MC11g1454 vs. NCBI nr
Match: XP_038890156.1 (threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1 [Benincasa hispida])

HSP 1 Score: 1082 bits (2797), Expect = 0.0
Identity = 551/633 (87.05%), Postives = 572/633 (90.36%), Query Frame = 0


Query: 4   MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVP 63
           MEDIEDLLIGGGG   APPGYRLP+ AVG+KPKKKN LK NP+ADDSSN+LSPTQNPLVP
Sbjct: 1   MEDIEDLLIGGGG---APPGYRLPINAVGVKPKKKNMLKFNPNADDSSNELSPTQNPLVP 60

Query: 64  KIPGTQTIYVKTFGCSHNQARSEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPSQSA 123
           KIPGTQTIYVKTFGCSHNQ+ SEYMAGQLSAFGYLLSDNPE+ADLWLINTCTVKSPSQSA
Sbjct: 61  KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSA 120

Query: 124 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVR- 183
           MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVR 
Sbjct: 121 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 180

Query: 184 ---------------KNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTV 243
                          KNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVR+V
Sbjct: 181 LNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRSV 240

Query: 244 INEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK 303
           INEGV+E+WLSSEDTGAYGRDIGVNLP LLNAIVAELPPDASTMLRIGMTNPPFILEHLK
Sbjct: 241 INEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLK 300

Query: 304 EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDII 363
           EIANVL HPCVYSFLHVPVQSGSD ILSAMNREYTV EFRTVVDTLTELVPGMQ+ATDII
Sbjct: 301 EIANVLSHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDII 360

Query: 364 CGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 423
           CGFPGETDEDFC+TINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV
Sbjct: 361 CGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 420

Query: 424 FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVG 483
           FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITS G
Sbjct: 421 FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSFG 480

Query: 484 RWSVFGDVIETISTRHHKTTTLEEMPIQDKVP-CCNTHEKCSSSTEPEPCACGPESCKAP 543
           RWSVFG+VIETIST+HHKTTTLE+ PI+DKVP C N HE C+ S EPE CACGPESCK  
Sbjct: 481 RWSVFGEVIETISTKHHKTTTLEDAPIRDKVPPCSNAHETCACSIEPESCACGPESCKGA 540

Query: 544 VTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQASG---- 603
           V  D+KDDSS NI L EEPKRKN++EW LRRRK HV KREERES  I SERKQA      
Sbjct: 541 VATDDKDDSSRNIPLPEEPKRKNLVEWALRRRKSHVPKREERESP-IVSERKQALAGGWL 600

Query: 604 -EWGFVDRILVGGILISIFAIVGVLFHLGSTTL 614
            EWG +DRILVGGILIS   I G+LFHLGSTTL
Sbjct: 601 DEWGVIDRILVGGILISTLTIFGLLFHLGSTTL 629

[Close]

BLAST of MC11g1454 vs. NCBI nr
Match: XP_023524910.1 (threonylcarbamoyladenosine tRNA methylthiotransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1079 bits (2790), Expect = 0.0
Identity = 552/634 (87.07%), Postives = 572/634 (90.22%), Query Frame = 0


Query: 4   MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVP 63
           MEDIEDLLIGGGG   APPGYRLP++AVG+KPKKKNKLK NPDADDSS+ L PTQN L P
Sbjct: 1   MEDIEDLLIGGGG---APPGYRLPISAVGVKPKKKNKLKPNPDADDSSSGLFPTQNLLAP 60

Query: 64  KIPGTQTIYVKTFGCSHNQARSEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPSQSA 123
           KIPGTQTIYVKTFGCSHNQ+ SEYMAGQLSAFGYLLS+NPEEADLWLINTCTVKSPSQSA
Sbjct: 61  KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSENPEEADLWLINTCTVKSPSQSA 120

Query: 124 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVR- 183
           MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVR 
Sbjct: 121 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 180

Query: 184 ---------------KNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTV 243
                          KNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV RVRTV
Sbjct: 181 LNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVNRVRTV 240

Query: 244 INEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK 303
           INEGV+E+WLSSEDTGAYGRDIGVNLP LLNAIVAELPPDASTMLRIGMTNPPFILEHLK
Sbjct: 241 INEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLK 300

Query: 304 EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDII 363
           EIANVLCHPCVYSFLHVPVQSGSD ILSAMNREYTV EFRTVVDTLTELVPGMQ+ATDII
Sbjct: 301 EIANVLCHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDII 360

Query: 364 CGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 423
           CGFPGETDEDFC+TINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV
Sbjct: 361 CGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 420

Query: 424 FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVG 483
           FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVI PE MLGTSA+VKITSVG
Sbjct: 421 FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIGPEAMLGTSALVKITSVG 480

Query: 484 RWSVFGDVIETISTRHHKTTTLEEMPIQDKVP-CCNTHEKCSSSTEPEPCACGPESC-KA 543
           RWSVFG+VIETI T+HHKTT LE+MPIQ KVP C N HE C+ S EPE CACG ESC K 
Sbjct: 481 RWSVFGEVIETIVTKHHKTTALEDMPIQAKVPPCSNAHETCACSVEPEACACGAESCCKG 540

Query: 544 PVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQASG--- 603
           PV LD KDDSS NI L EEPKRKN++EWLLR+RK HV KREERES  + SERKQAS    
Sbjct: 541 PVALDEKDDSSRNIPLPEEPKRKNLVEWLLRKRKSHVPKREERESP-VVSERKQASAGGS 600

Query: 604 --EWGFVDRILVGGILISIFAIVGVLFHLGSTTL 614
             EWG VDRILVGGILISIF IVG+LFHLGSTTL
Sbjct: 601 MSEWGVVDRILVGGILISIFTIVGLLFHLGSTTL 630

[Close]

BLAST of MC11g1454 vs. ExPASy TrEMBL
Match: A0A6J1DPN3 (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Momordica charantia OX=3673 GN=LOC111022375 PE=3 SV=1)

HSP 1 Score: 1212 bits (3135), Expect = 0.0
Identity = 612/633 (96.68%), Postives = 615/633 (97.16%), Query Frame = 0


Query: 1   MQAMEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNP 60
           MQAMEDIEDLLIGGGGGGGAPPGYRLPLTAVG+KPKKKNKLKSNPDADDSSNQLSPTQNP
Sbjct: 1   MQAMEDIEDLLIGGGGGGGAPPGYRLPLTAVGLKPKKKNKLKSNPDADDSSNQLSPTQNP 60

Query: 61  LVPKIPGTQTIYVKTFGCSHNQARSEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPS 120
           LVPKIPGTQTIYVKTFGCSHNQ+ SEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPS
Sbjct: 61  LVPKIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPS 120

Query: 121 QSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE 180
           QSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE
Sbjct: 121 QSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE 180

Query: 181 VR----------------KNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRV 240
           VR                KNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRV
Sbjct: 181 VRLLNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRV 240

Query: 241 RTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILE 300
           RTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILE
Sbjct: 241 RTVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILE 300

Query: 301 HLKEIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIAT 360
           HLKEIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIAT
Sbjct: 301 HLKEIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIAT 360

Query: 361 DIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSREL 420
           DIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSREL
Sbjct: 361 DIICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSREL 420

Query: 421 TSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKIT 480
           TSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVK+T
Sbjct: 421 TSVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKVT 480

Query: 481 SVGRWSVFGDVIETISTRHHKTTTLEEMPIQDKVPCCNTHEKCSSSTEPEPCACGPESCK 540
           SVGRWSVFGDVIETISTRHHKTTTLEEMPIQDKVPCCNTHEKCSSSTEPEPCACGPESCK
Sbjct: 481 SVGRWSVFGDVIETISTRHHKTTTLEEMPIQDKVPCCNTHEKCSSSTEPEPCACGPESCK 540

Query: 541 APVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQASGEW 600
           APVTLDNKDDSSSNISLAEEPKRKNVIEWL RRRKRHVAKREERESSGIASERKQASGEW
Sbjct: 541 APVTLDNKDDSSSNISLAEEPKRKNVIEWLPRRRKRHVAKREERESSGIASERKQASGEW 600

Query: 601 GFVDRILVGGILISIFAIVGVLFHLGSTTLLLL 617
           GFVDRILVGGILISIFAIVGVLFHLGSTTLLLL
Sbjct: 601 GFVDRILVGGILISIFAIVGVLFHLGSTTLLLL 633

[Close]

BLAST of MC11g1454 vs. ExPASy TrEMBL
Match: A0A6J1GBS1 (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucurbita moschata OX=3662 GN=LOC111452531 PE=3 SV=1)

HSP 1 Score: 1083 bits (2802), Expect = 0.0
Identity = 554/634 (87.38%), Postives = 575/634 (90.69%), Query Frame = 0


Query: 4   MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVP 63
           MEDIEDLLIGGGGGG APPGYRLP++AVG+KPKKKNKLKSNPDADDSS+ L PTQN L P
Sbjct: 1   MEDIEDLLIGGGGGG-APPGYRLPISAVGVKPKKKNKLKSNPDADDSSSGLFPTQNLLAP 60

Query: 64  KIPGTQTIYVKTFGCSHNQARSEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPSQSA 123
           KIPGTQTIYVKTFGCSHNQ+ SEYMAGQLSAFGYLLS+NPEEADLWLINTCTVKSPSQSA
Sbjct: 61  KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSENPEEADLWLINTCTVKSPSQSA 120

Query: 124 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVR- 183
           MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVR 
Sbjct: 121 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 180

Query: 184 ---------------KNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTV 243
                          KNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV RVRTV
Sbjct: 181 LNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVNRVRTV 240

Query: 244 INEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK 303
           INEGV+E+WLSSEDTGAYGRDIGVNLP LLNAIVAELPPDASTMLRIGMTNPPFILEHLK
Sbjct: 241 INEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLK 300

Query: 304 EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDII 363
           EIANVLCHPCVYSFLHVPVQSGSD ILSAMNREYTV EFRTVVDTLTELVPGMQ+ATDII
Sbjct: 301 EIANVLCHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDII 360

Query: 364 CGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 423
           CGFPGETDEDFC+TINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV
Sbjct: 361 CGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 420

Query: 424 FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVG 483
           FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVI  ETMLGTSA+VKITSVG
Sbjct: 421 FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIGHETMLGTSALVKITSVG 480

Query: 484 RWSVFGDVIETISTRHHKTTTLEEMPIQDKVP-CCNTHEKCSSSTEPEPCACGPESC-KA 543
           RWSVFG+VIETI T+HHKTT LE+MPIQ KVP C + HE C+ S EPE CACGPESC K 
Sbjct: 481 RWSVFGEVIETIVTKHHKTTALEDMPIQAKVPPCSDAHETCACSVEPEACACGPESCCKG 540

Query: 544 PVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQASG--- 603
           PV LD KDDSS NI L EEPKRKN++EWLLR+RK H  KREERES  + SERKQAS    
Sbjct: 541 PVALDEKDDSSRNIPLPEEPKRKNLVEWLLRKRKSHAPKREERESP-VVSERKQASAGGS 600

Query: 604 --EWGFVDRILVGGILISIFAIVGVLFHLGSTTL 614
             EWG VDRILVGGILISIF IVG+LFHLGSTTL
Sbjct: 601 MDEWGVVDRILVGGILISIFTIVGLLFHLGSTTL 632

[Close]

BLAST of MC11g1454 vs. ExPASy TrEMBL
Match: A0A6J1KEZ5 (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucurbita maxima OX=3661 GN=LOC111492654 PE=3 SV=1)

HSP 1 Score: 1082 bits (2797), Expect = 0.0
Identity = 552/634 (87.07%), Postives = 573/634 (90.38%), Query Frame = 0


Query: 4   MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVP 63
           MEDIEDLLIGGGGGGGAPPGYRLP++AVG+K KKKNKLK NPDADDSS+ L PTQN L P
Sbjct: 1   MEDIEDLLIGGGGGGGAPPGYRLPISAVGVKSKKKNKLKPNPDADDSSSGLFPTQNLLAP 60

Query: 64  KIPGTQTIYVKTFGCSHNQARSEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPSQSA 123
           KIPGTQTIYVKTFGCSHNQ+ SEYMAGQLSAFGYLLS+NPEEADLWLINTCTVKSPSQSA
Sbjct: 61  KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSENPEEADLWLINTCTVKSPSQSA 120

Query: 124 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVR- 183
           MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVR 
Sbjct: 121 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 180

Query: 184 ---------------KNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTV 243
                          KNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV RVRTV
Sbjct: 181 LNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVNRVRTV 240

Query: 244 INEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK 303
           INEGV+E+WLSSEDTGAYGRDIGVNLP LLNAIVAELPPDASTMLRIGMTNPPFILEHLK
Sbjct: 241 INEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLK 300

Query: 304 EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDII 363
           EIANVLCHPCVYSFLHVPVQSGSD ILSAMNREYTV EFRTVVDTLTELVPGMQ+ATDII
Sbjct: 301 EIANVLCHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDII 360

Query: 364 CGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 423
           CGFPGETDEDFC+TINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV
Sbjct: 361 CGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 420

Query: 424 FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVG 483
           FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVI  E MLGTSA+VKITSVG
Sbjct: 421 FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIGHEAMLGTSALVKITSVG 480

Query: 484 RWSVFGDVIETISTRHHKTTTLEEMPIQDKVP-CCNTHEKCSSSTEPEPCACGPESC-KA 543
           RWSVFG+VIETI T+HHKTT LE+MPIQ KVP C N+HE C+ S EPE CACGPESC K 
Sbjct: 481 RWSVFGEVIETIVTKHHKTTALEDMPIQAKVPPCSNSHETCACSVEPEACACGPESCCKG 540

Query: 544 PVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQASG--- 603
           PV LD KDDSS NI L EEPKRKN++EWLLR+RK HV KREE ES  + S RKQAS    
Sbjct: 541 PVALDEKDDSSRNIPLPEEPKRKNLVEWLLRKRKSHVPKREEGESP-VVSGRKQASAGGS 600

Query: 604 --EWGFVDRILVGGILISIFAIVGVLFHLGSTTL 614
             EWG VDRILVGGILISIF IVG+LFHLGSTTL
Sbjct: 601 MSEWGVVDRILVGGILISIFTIVGLLFHLGSTTL 633

[Close]

BLAST of MC11g1454 vs. ExPASy TrEMBL
Match: A0A0A0KSZ4 (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucumis sativus OX=3659 GN=Csa_5G652200 PE=3 SV=1)

HSP 1 Score: 1045 bits (2702), Expect = 0.0
Identity = 534/632 (84.49%), Postives = 562/632 (88.92%), Query Frame = 0


Query: 4   MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVP 63
           MEDIEDLLIGGGGG  APPGYRLP+TAVG+KPKKKN  KSN  ADDSSN+ SP QNPLVP
Sbjct: 1   MEDIEDLLIGGGGG--APPGYRLPITAVGVKPKKKNMFKSNASADDSSNEPSPIQNPLVP 60

Query: 64  KIPGTQTIYVKTFGCSHNQARSEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPSQSA 123
           KIPGTQTIYVKTFGCSHNQ+ SEYMAGQLSAFGYLLSDNPE+ADLWLINTCTVKSPSQSA
Sbjct: 61  KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSA 120

Query: 124 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVR- 183
           MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVR 
Sbjct: 121 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 180

Query: 184 ---------------KNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTV 243
                          KNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV+RVR+V
Sbjct: 181 LNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVRRVRSV 240

Query: 244 INEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK 303
           INEGV+E+WLSSEDTGAYGRDIGVNLP LLNAIV+ELP DASTMLRIGMTNPPFILEHLK
Sbjct: 241 INEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVSELPSDASTMLRIGMTNPPFILEHLK 300

Query: 304 EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDII 363
           EIA VL HPCVYSFLHVPVQSGSDAILSAMNREYTV EFRTVVDTLTELVPGMQIATDII
Sbjct: 301 EIAKVLSHPCVYSFLHVPVQSGSDAILSAMNREYTVSEFRTVVDTLTELVPGMQIATDII 360

Query: 364 CGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 423
           CGFPGETDEDFC+TINLIKEY LPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV
Sbjct: 361 CGFPGETDEDFCETINLIKEYNLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 420

Query: 424 FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVG 483
           FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSA VKITS+G
Sbjct: 421 FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSATVKITSIG 480

Query: 484 RWSVFGDVIETISTRHHKTTTLEEMPIQDKV-PCCNTHEKCSSSTEPEPCACGPESCKAP 543
           RWSVFG+VIE IST+HHKTTTLE+   QDKV PC NTHE C+ STEPE CACG ESCK  
Sbjct: 481 RWSVFGEVIEIISTKHHKTTTLEDTLTQDKVSPCSNTHETCACSTEPESCACGLESCKGA 540

Query: 544 VTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQ--ASG-- 603
           V + ++ +SS N+   EEPKRKN+IEW+LRRRK HV  + E   + I +ERKQ  A G  
Sbjct: 541 VAVGDEVNSSRNVPSPEEPKRKNLIEWVLRRRKSHVLPKREETENPIVTERKQTLAGGRL 600

Query: 604 -EWGFVDRILVGGILISIFAIVGVLFHLGSTT 613
            EWG VD+ILVGGILIS F I G+LFHLGSTT
Sbjct: 601 DEWGVVDKILVGGILISTFTIFGLLFHLGSTT 630

[Close]

BLAST of MC11g1454 vs. ExPASy TrEMBL
Match: A0A1S3CA89 (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucumis melo OX=3656 GN=LOC103498722 PE=3 SV=1)

HSP 1 Score: 1044 bits (2699), Expect = 0.0
Identity = 538/633 (84.99%), Postives = 563/633 (88.94%), Query Frame = 0


Query: 4   MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPKKKNKLKSNPDADDSSNQLSPTQNPLVP 63
           MEDIEDLLIGGGG   APPGYRLP+TAVG+KPKKKN  KSNP ADDSSNQ SPTQNPLVP
Sbjct: 1   MEDIEDLLIGGGG---APPGYRLPITAVGVKPKKKNLFKSNPSADDSSNQPSPTQNPLVP 60

Query: 64  KIPGTQTIYVKTFGCSHNQARSEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPSQSA 123
           KIPGTQTIYVKTFGCSHNQ+ SEYMAGQLSAFGYLLSDNPE+ADLWLINTCTVKSPSQSA
Sbjct: 61  KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSA 120

Query: 124 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVR- 183
           MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVR 
Sbjct: 121 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 180

Query: 184 ---------------KNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRTV 243
                          KNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVR+V
Sbjct: 181 LNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRSV 240

Query: 244 INEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEHLK 303
           INEGV+E+WLSSEDTGAYGRDIGVNLP LLNAIVAELPPDASTMLRIGMTNPPFILEHLK
Sbjct: 241 INEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLK 300

Query: 304 EIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDII 363
           EIA VL HPCVYSFLHVPVQSGSDAILSAMNREYTV EFRTVVDTLTELVPGMQIATDII
Sbjct: 301 EIAKVLSHPCVYSFLHVPVQSGSDAILSAMNREYTVSEFRTVVDTLTELVPGMQIATDII 360

Query: 364 CGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 423
           CGFPGETDEDFC+TINLIKEY LPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV
Sbjct: 361 CGFPGETDEDFCETINLIKEYNLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 420

Query: 424 FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSVG 483
           FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAM KITS+G
Sbjct: 421 FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMAKITSIG 480

Query: 484 RWSVFGDVIETISTRHHKTTTLEEMPIQDKV-PCCNTHEKCSSSTEPEPCACGPESCKAP 543
           RWSVFG+VIETIST+HHKTT LE+   +DKV PC NTHE C+ STEPE CAC   SCK  
Sbjct: 481 RWSVFGEVIETISTKHHKTT-LEDTLTRDKVSPCSNTHETCACSTEPESCACELGSCKGA 540

Query: 544 VTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQ--ASG-- 603
           V++ ++ DSS NI L EEPKRKN+IEW+LRRRK  V  + E   + I +ERKQ  A G  
Sbjct: 541 VSVGDEVDSSRNIPLPEEPKRKNLIEWVLRRRKSQVMPKREETENPIVTERKQTLAGGRL 600

Query: 604 -EWGFVDRILVGGILISIFAIVGVLFHLGSTTL 614
            EWG VD ILVGGILIS F I G+LFH+GSTTL
Sbjct: 601 DEWGVVDMILVGGILISTFTIFGLLFHVGSTTL 629

[Close]

BLAST of MC11g1454 vs. TAIR 10
Match: AT1G72090.1 (Methylthiotransferase )

HSP 1 Score: 807.0 bits (2083), Expect = 1.1e-233
Identity = 430/630 (68.25%), Postives = 483/630 (76.67%), Query Frame = 0


Query: 4   MEDIEDLLIGGGGGGGAPPGYRLPLTAVGIKPK--KKNKLKSNPDADDSSNQLSPTQNPL 63
           MEDIEDLL   GG GGAPPG+RLPL AVGI PK  K  ++ S PD   +SN+ S    P 
Sbjct: 1   MEDIEDLL--AGGVGGAPPGFRLPLNAVGINPKTNKSKRISSKPDQITASNRDSLA--PP 60

Query: 64  VPKIPGTQTIYVKTFGCSHNQARSEYMAGQLSAFGYLLSDNPEEADLWLINTCTVKSPSQ 123
             KIPGTQTIY+KTFGCSHNQ+ SEYMAGQLSAFGY L++ PEEADLWLINTCTVKSPSQ
Sbjct: 61  SMKIPGTQTIYIKTFGCSHNQSDSEYMAGQLSAFGYALTEVPEEADLWLINTCTVKSPSQ 120

Query: 124 SAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHE- 183
           SAM TLIT+ ++ KKPLV+AGCVPQGSRDLKELEGVS+VGVQQIDRVVE+VEETLKGHE 
Sbjct: 121 SAMSTLITRGRSGKKPLVIAGCVPQGSRDLKELEGVSVVGVQQIDRVVEIVEETLKGHEV 180

Query: 184 ---------------VRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVR 243
                          VR+N FIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV+RVR
Sbjct: 181 RLLTRKTLPALDLPKVRRNNFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVERVR 240

Query: 244 TVINEGVKEVWLSSEDTGAYGRDIGVNLPSLLNAIVAELPPDASTMLRIGMTNPPFILEH 303
           TVI+EGVKE+WLSSEDTGAYGRDIGVNLP LLNAIV ELP D STMLRIGMTNPPFILEH
Sbjct: 241 TVISEGVKEIWLSSEDTGAYGRDIGVNLPILLNAIVKELPSDQSTMLRIGMTNPPFILEH 300

Query: 304 LKEIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATD 363
           LKEIA VL HPCVY+FLHVPVQSGSD++L+AMNREYT  EFRTVVDTLTELVPGMQIATD
Sbjct: 301 LKEIAAVLRHPCVYTFLHVPVQSGSDSVLTAMNREYTASEFRTVVDTLTELVPGMQIATD 360

Query: 364 IICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELT 423
           IICGFPGETDEDF QT+ LIK+YK PQVHISQFYPRPGTPAA+MKKV S IVK+RSRELT
Sbjct: 361 IICGFPGETDEDFSQTVELIKDYKFPQVHISQFYPRPGTPAAKMKKVQSKIVKQRSRELT 420

Query: 424 SVFEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITS 483
           SVFEAF PY GME R ERIWITE+A DGIHLVGHTKGY+QVLV  PE+MLGTSAM +ITS
Sbjct: 421 SVFEAFAPYTGMECREERIWITEVATDGIHLVGHTKGYVQVLVTGPESMLGTSAMARITS 480

Query: 484 VGRWSVFGDVIETISTRHHKTTTLEEMPIQDKVPCCNTHEKCSSSTEPEPCACGPESCKA 543
           VGRWSVFG+VIET S+ + +T + EE     K+PC +    C      E C C  ESC  
Sbjct: 481 VGRWSVFGEVIETFSSANRETKSREE----TKLPCSSNVSTC------ETCTCSAESC-G 540

Query: 544 PVTLDNKDDSSSNISLAEEPKRKNVIEWLLRRRKRHVAKREERESSGIASERKQASGEWG 603
                   + S NIS  ++ K K              +K+EE+E   +       +  WG
Sbjct: 541 EERSGEACNISGNISGQDDNKGK--------------SKKEEKEVQEVVVPGSSVA-NWG 600

Query: 604 FVDRILVGGILISIFAIVGVLFHLGSTTLL 616
           F+D+ LV G+ +S   I+ +L  +    LL
Sbjct: 601 FIDKALVCGVFVSSVTILVLLISIACRVLL 600

[Close]

BLAST of MC11g1454 vs. TAIR 10
Match: AT4G36390.1 (Methylthiotransferase )

HSP 1 Score: 115.9 bits (289), Expect = 1.1e-25
Identity = 111/472 (23.52%), Postives = 196/472 (41.53%), Query Frame = 0


Query: 57  TQNPLVPKIPGTQTIYVKTFGCSHNQARSEYMAGQLSAFGYL-LSDNPEEADLWLINTCT 116
           +++ L   I     IY +T+GC  N    E +   +   GY  +  +PE A++  +NTC 
Sbjct: 117 SESTLDSDIASKGRIYHETYGCQMNINDMEIVLAIMKNSGYKEVVTDPESAEVIFVNTCA 176

Query: 117 VKSPSQS----------------AMDTLITKCKNAKKP-LVVAGCVPQGSRDLKELEGVS 176
           ++  ++                  ++    + K+ K P +VV GC+ +  +D K L+   
Sbjct: 177 IRENAEQRVWQRLNYFWFLKREWKVNAATGRAKSLKPPKVVVLGCMAERLKD-KILDSDK 236

Query: 177 IVGV-------QQIDRVVEVVEETLKGHEV---RKNKFIEILPINV-------------G 236
           +V V       + + R++E V+   KG       +  + +I P+ +             G
Sbjct: 237 MVDVVCGPDAYRDLPRLLEEVDYGQKGINTLLSLEETYADISPVRISENSITAFVSVMRG 296

Query: 237 CLGACTYCKTKHARGHLGSYTVDSLVKRVRTVINEGVKEVWLSSEDTGAYGRD------- 296
           C   C +C     RG   S  V+S+++ V  +   GVKEV L  ++  +Y  D       
Sbjct: 297 CNNMCAFCIVPFTRGRERSRPVESIIREVGELWESGVKEVTLLGQNVNSYNDDSADRESG 356

Query: 297 -----------------IGVNLPSLLNAIVAELPPDASTMLRIGMTNP-----PFILEHL 356
                            +G+    LL+ +  E P      +R   T+P     P  L +L
Sbjct: 357 ANWEYSEGFSSRCKVKNMGLRFADLLDRLSVEFPE-----MRFRFTSPHPKDYPDELLYL 416

Query: 357 KEIANVLCHPCVYSFLHVPVQSGSDAILSAMNREYTVDEFRTVVDTLTELVPGMQIATDI 416
               + +C     + +H+P QSG+  IL  M R YT + +  +V  +  ++P + I +D 
Sbjct: 417 MRDRHNIC-----NLIHLPAQSGNSRILEQMRRGYTREAYLDLVKKIRSIIPDVAITSDF 476

Query: 417 ICGFPGETDEDFCQTINLIKEYKLPQVHISQFYPRPGTPAAR--MKKVPSAIVKKRSREL 452
           I GF GET+E+  +T++L++       ++  +  R  T A R     VP  + ++R  EL
Sbjct: 477 ITGFCGETEEEHQETLSLVRAVGYDMAYMFAYSMREKTHAHRNYTDDVPEEVKQRRLTEL 536

The following BLAST results are available for this feature:

BLAST of MC11g1454 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q291H5	1.6e-133	50.10	Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Drosophila pseudoobscur...	[more]
Q7K4W1	7.9e-133	48.75	Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Drosophila melanogaster...	[more]
Q6P4Y0	6.7e-132	49.58	Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Xenopus tropicalis OX=8...	[more]
Q6NS26	9.7e-131	49.58	Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Xenopus laevis OX=8355 ...	[more]
Q5VV42	1.2e-128	47.78	Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Homo sapiens OX=9606 GN...	[more]

BLAST of MC11g1454 vs. NCBI nr


Match Name	Identity	Description
XP_022155234.1	96.68	threonylcarbamoyladenosine tRNA methylthiotransferase [Momordica charantia]	[more]
XP_022949069.1	87.38	threonylcarbamoyladenosine tRNA methylthiotransferase [Cucurbita moschata]	[more]
XP_022997808.1	87.07	threonylcarbamoyladenosine tRNA methylthiotransferase [Cucurbita maxima]	[more]
XP_038890156.1	87.05	threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1 [Benincasa hisp...	[more]
XP_023524910.1	87.07	threonylcarbamoyladenosine tRNA methylthiotransferase [Cucurbita pepo subsp. pep...	[more]

BLAST of MC11g1454 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1DPN3	96.68	Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Momordica charantia OX=...	[more]
A0A6J1GBS1	87.38	Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucurbita moschata OX=3...	[more]
A0A6J1KEZ5	87.07	Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucurbita maxima OX=366...	[more]
A0A0A0KSZ4	84.49	Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucumis sativus OX=3659...	[more]
A0A1S3CA89	84.99	Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucumis melo OX=3656 GN...	[more]

BLAST of MC11g1454 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G72090.1	1.1e-233	68.25	Methylthiotransferase	[more]
AT4G36390.1	1.1e-25	23.52	Methylthiotransferase	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006638	Elp3/MiaB/NifB	SMART	SM00729	MiaB	coord: 186..407 e-value: 2.1E-40 score: 150.2
IPR005839	Methylthiotransferase	TIGRFAM	TIGR00089	TIGR00089	coord: 70..471 e-value: 9.6E-93 score: 309.2
IPR007197	Radical SAM	PFAM	PF04055	Radical_SAM	coord: 193..365 e-value: 7.5E-20 score: 72.0
IPR007197	Radical SAM	SFLD	SFLDS00029	Radical_SAM	coord: 186..398 e-value: 3.6E-27 score: 89.8
IPR007197	Radical SAM	PROSITE	PS51918	RADICAL_SAM	coord: 179..415 score: 28.84133
IPR023404	Radical SAM, alpha/beta horseshoe	GENE3D	3.80.30.20	tm_1862 like domain	coord: 188..414 e-value: 2.9E-69 score: 235.1
IPR013848	Methylthiotransferase, N-terminal	PFAM	PF00919	UPF0004	coord: 71..149 e-value: 1.5E-18 score: 66.6
IPR013848	Methylthiotransferase, N-terminal	PROSITE	PS51449	MTTASE_N	coord: 69..177 score: 28.460508
IPR006466	MiaB-like tRNA modifying enzyme, archaeal-type	TIGRFAM	TIGR01578	TIGR01578	coord: 71..475 e-value: 5.6E-130 score: 431.8
IPR038135	Methylthiotransferase, N-terminal domain superfamily	GENE3D	3.40.50.12160		coord: 70..187 e-value: 4.0E-27 score: 96.4
None	No IPR available	SFLD	SFLDG01082	B12-binding_domain_containing	coord: 186..398 e-value: 3.6E-27 score: 89.8
None	No IPR available	PIRSR	PIRSR000167-1	PIRSR000167-1	coord: 306..390 e-value: 3.2E-6 score: 24.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 46..63
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 35..63
None	No IPR available	PANTHER	PTHR11918	RADICAL SAM PROTEINS	coord: 4..510
None	No IPR available	CDD	cd01335	Radical_SAM	coord: 190..395 e-value: 1.6148E-5 score: 44.2466
None	No IPR available	SUPERFAMILY	102114	Radical SAM enzymes	coord: 180..410
IPR020612	Methylthiotransferase, conserved site	PROSITE	PS01278	MTTASE_RADICAL	coord: 190..210
IPR002792	TRAM domain	PROSITE	PS50926	TRAM	coord: 415..477 score: 9.800363

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC11g1454.1	MC11g1454.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0035600	tRNA methylthiolation
biological_process	GO:0006400	tRNA modification
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0035598	N6-threonylcarbomyladenosine methylthiotransferase activity
molecular_function	GO:0061712	tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0035596	methylthiotransferase activity
molecular_function	GO:0016740	transferase activity