MC11g1352 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC11g1352
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	2-isopropylmalate synthase
Location	MC11: 17017351 .. 17040715 (+)
RNA-Seq Expression	MC11g1352
Synteny	MC11g1352

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GGCTCCATAGAAAGGAAAGAAATTAAGTAAAATTTGGCAACCCTATATTAGCTTAGCCGCCGTGAATGCGAAAGAGGAGGAGAAAATTTCTTCTGTGAGTTCTTTTTCCTGCTTTCTCTCGGCGAGGTTGCTGTTGTTGTGTTTAAGATGGCCATGGCGACAGCATTTTCAAGCCCGAATCACTGTTTTGGTCGGCAATGGAACTCGCAATTTCCTACCAGCGGTCACATTTATCGTCGGAACTTGCAGAATCCGTCGTCATTTAGGTCGCCGGCGGGCCGTGTTTGTTGTAGTGAGGCACAGAGCATAAGCATAGCTTCCGATTCCAAACCGAATTCATCTGTTCAACGCCCGCCGTACATCCCCAATCGCATCCCTGACCCTTCCTATGTTCGCATTTTTGATACGACTCTCCGCGATGGCGAGCAGTCCCCAGGTGCGTCCCTCACCTCTAAAGAGAAGCTGGATATTGCTCGTCAGCTCGCCAAGCTCGGCGTGGACATAATTGAGGCTGGATTCCCAGCTGCTTCGAAGGAAGACTTTGAGGCTGTGAAGATCATTGCCAAAGAGGTGGGAAATGTTGTCGACCAGGACGGGTACGTGCCTGTAATTTGTGGTCTCTCGAGGTGCAATGAGAAGGATATTCGAACTGCATGGGAGGCCGTGAAGTACGCTAAGAGACCGCGGATACATACGTTTATTGCAACTAGCGAAATTCATATGAAGTATAAGCTGAGGAAGACGAGGGAGGAAGTGATTGAGATTGCTCGAAGTATGGTGCGGTTTGCACGAAGTTTGGGCTGTGATGATGTTGAGTTCAGCCCCGAGGATGCTGGGAGGTTTGACCTTGATCTCCACTTTTTAAAATTTCAAATTTGGTTTCATCTAATTTATGCAAACGAAAGAGTAAGCTGACTTTGCATGCTCCTTGGAAGTTCAAAACCTCTCAATCATTATTGATGTTTGCATCAAGTCGTTAATCAGTTTAGGTTTATGAAGCCTGAAGTTGTAAAATGTGAATTTGTTTTTTTTTTAGATAAGAAACATTTCATTAAGAAGGAAAACCAAAAAACGAACTAGGACAAGGGACGTCCCTTTCCCTAAAGAACACTAAAAGAGAAGGGCTCTCCAATCTGGTATGATCTTAGAGAGGCTGTAATTACTTGTTCCTATAACACTACCAAGATGTCATAATATGAACACGGTTCCTTTTCCAGCCATAAGTGGCATCAGAGAGCTCTAAAAGCACGGCCCCATAACTCTTTGCATTTACCGCTGAAGCTCCGTCCTTGAGTACATTCTGTCAACCAATCATCAATCATCTCAGGCAAGCAAGTAGAAATAGGGAATACATCGAACAAAAACTGCCACCCCCTTTTAGCAAAAGAATAATGAAAGAAAAGGTGATCTAAAGGATATGAAAATTAATAGGATATGAAAAATCTTGAATTGGTGTAGTTTGTTTGTTTTTTGTTTTTTGATTTGTAAAAGTTGGGGGTTTCACAATTTTGATTTATATAATTTGGGTTTACATAGGCTAGTGTGGTTAACAAACCTTTAACTAGTTTTGGCCTTTTTGTTGGCTGGATTAATACAATTAATTTAATGGGACAATGCTTAGACCCTATGTATGGTTGTTATCTAACTTGTACAGGTTGCCTATATATTTAATGCCCTATTTATCTGTTGCTGTTCATGAACGATATGTATCTTGTAAAATAATGAAGATAAAATATTTATTTTTCTGCTAGACATTACAAATTTATATTTGCAACTTTTCAAATTCTAAATTCTCGCACATATAAATATTTGCCTTTTTTATTTTCTGGCGTCCTATCAGATCTGATAGGGAGTTTCTTTACGAAATTCTGGGTGAAGTTATAAAGGCTGGGGCAACTACTCTCAACATACCAGATACTGTGGGTTATACCATGCCGGTTGAATTTGGGGCGTTGATTGCTGATATAAAAACTAATACGCCTGGCATTGAAAATGTTATCATTTCGACACACTGCCAGAATGATCTTGGACTCGCAACTGCCAACACAGTAGCAGTAAGTAATGCCTTTTTCTTCCCTTTTAATCCTGTTGATTGAACTGGACGTTTAATTTGGAACTACCCGAAGATAGTAGGAAAGTAAAAAGGATTACAAATATCATACTATAACACTAAGAAAGAAAAGGAAAAATATAAAAAAAAACAAGACGTGAAAATGATGAGGCTGCATCGTTGGAATATATACCTATAGTTATGGTCATATTTTAAGGATTTATATGCTCAAGAGTTTGCATCTTAAATTATGAAGAAGGGATAAGATATACATTTTGTGGCTTGATCTTTGTCCCTCGAAGTACAAAAGTTGTACTTGTGGAGGACAAACTTCTTCAGCCGCTCCTGGAAATTTGGCGAATTGCTAAGGGATTTTAGTGTGAACTATAAATCACTCTTCCACCTATGACCTATCTAAATGCTGTTACGACTTCATCTATTTCCTTAAGTAAATTATGTTTGATTCCTCTCATCTTTTTTCAGGGAGCATGTGCAGGTGCAAGGCAAGTTGAGGTCACAATTAATGGCATTGGTGAGAGAGCTGGAAATGCTTCGCTGGAGGAGGCAAGTTATTTATTTAATTTATTTCCTACTTATTCTTTCATTTATTTTGAAATAATAATCATTCAAGAAGGTTGTGTATAATAATAAGTTTTTAAACTTTAATAAATGTCTAACAAGCTCCTAAACTTTCAATTTTATATCTAATAGCTTCTTGAACTCTAATATATTCTTGACTAATGAGGCTTCTTTGACTATATTTTTCCACAATAATTTAGCACCAATTTTCATCCCATGACCACACCACATACCAACATAAAAACGTTATTTCAAAGCTTTCCTCTCCAAATACAAATTGGAAACAATAACTATAACTTGAAAATAGATACCACTAACAATATATAGTTCGCCATAGCCTCCTCTTGGAGCTCTTGCTCAGGGGAGTTTTGTTATTATTCTCCTAATGTTGCTTTATATTGGAGTGTTTTTTAAGCTCCTTTGGAGTTCTTGTACATTATTTACATAGAGAAGAAAAGTTTTGTTTCTTATAAATTTCTTAAGCACAATGTTGGCTGTACTTTGTTCTTTTCCTCTCCTCCTACAGCCCAACAATATGCTACTTTCTTTAATTTTTCTCTTTTGATAGGAAACAATTTTCATTGATGATATGAAATTATACAAAATGATCAAAACCTATACAATGAAGTTAAAAAATCTTCTCCAAATGGATATAAGAGACTTCAAGCTATAATTGCTAAAAGGAGAGACATTTTTACACCATGTTAGAGCCAAAAAAGAAATGAAATCAATCAATCAAGCGTTGCCAAATGACCCCCAACTTCTCCTTGAGGCAACTCTTATAGGACACTAATAAGACTGAATAGGACGAATTGCTCATTCTCATTAAATGTAATAGAGGAACAACTGTCCATATATACACACCACACAGGTGTCACTTAAATAATGTGACAAAATAACAACTTAACTATCTAACCATACAACTAACTAAGATAATATTAATACACTCATACTCCCCCCTCAAGATGGGGAAAAAACATCAAGAGACCCATCTTGCATAGCAGAGAGTTAAGCGTGACAGCAGGCAAGGCTTTTGTAAAAACATCAGCTAGTTGTAGGTGCGAACGAATAGGTAGAAGCCGAATAACACGAGATTTCTCCCGAACGAAATGTCAATCAAGCTCTATGTGCTTGGTACGCTCATGAAAACTCGGGTTAGTAGCAATGTGGATGACTGCGCATTATCACAATATAAAAGTGCAGGAGATGATATCATGATATAGGAACCTGCACTCATGTAGCAAATTGGTTAACCAAATAAGTTCACAAGTTGTAGCTGCCAACGCTCCGTACTCGGCTTCAACAGAGGATCGAGAAATTGTGGTATGCTTTTTTGCCTTCCAAGACACCAAGGAATCACCTAAGAAAACACAGAACCCAGTGGTACAACGCTGAGTATCAAGACACGATCCCAATCTGCAATTAGAGAATGATTTCAATTGAAAAGAAGAAGTAGCCAAAAAGATTCCACGACCTGGGGCATTTTTCAAATAGCGAAGCAGATAACATATAGCATGAAGATGAGCTTTGCTAGGTTGGGAAACAAACTAACTTAACTTGTGAACGACATACGTGATATCAAGCCGAGATATTGTCAGATAGAGAAGACGACTAACAAGTCTTCTAAAAACAGAAGGATCATCAAGATCAGTATTTCGAAGGTGAGTAGAAGGATCCATGGGAACAGCCACAGACTTGCATCCCAATAATCCAGTATCTTCTAATAAAGAGAGTGTATAATGCCTCTGAGATAAAGAAATACCACGATCTTCTAATAAAGAGAGTGTATAATGCCTCTGAGATAAAGAAATACCACGAGATGACCGTGCTAGCTCAAGGCCAAGAAAATACTTAAGTTGGTCGAGATCTTTCAGCTTAAAAGTCTGGTCAAGAAGACATTTCAAATCATTAAGGGCCCGTTTGGTTGGAAACTCGGATTCTGTTTTTGTTTTCAGATTCACTGAATCAAATGAGTGTTTGGCAGAATTTTTGAGTTTCGTTTTTTAAAACTGTTTTCGGATTCTGTGATTCAAACAGTAGAAATTTTGAAAACGACTTTTTTATGTTTTCAGTGTTTTCAGATTCAGAATTTGAAATTTTTAAGATGCAGAATTACATTAAAAAGATAAAAACTTTGATAAAATATATTACTTCATATTATAAACTCATTTTATGCTTGTTAAAATTGAAACATGTAAAATATAATGTATTCTAAATTATATAACCCTGTAAAATAATAATCATACAAAATTCATAAAAAAATGTTAATAAATTACTTAATATAATTTAGATTAAACTTTGTAACTAATTTTAGTTTAGAAATTTAGTTTGTAACTAATTTTGCAACTGGAGAGAATGTCTCCATAAAATCAAGGCCGTCTAGCTGAGTATACCCCTTTGCAACCAATTGTGCTTTGTAACGCTCGATGAAACCATCAGAATTATGTTTAATTTTGTAAATCTATTTACATCCAACAGTATGATGACTAGCTGGAAGTAGAACAACGGACCAAGTTTTATTAGACTCCATGGCCGAAAGTTCAACAGTAATAGCATCACGCCAATGTTGATAAAACTGTGGTTCCTCAGTGGAAGAGATACTGGCGGCCAGAGAATAATGAGAAGATGATAATCTGGAATAAGAAACATGATGCTGAATAGGATGTTTAGTAAACCAAGTGGGCAAAGAATCACCATTTAAAAGAGAGCACAGTGATAGTCCTTGAGATAAGAAAGGAGCCTTAGAAGCTCGAAGGGACCTTCGTAAAGGTGGTGGAATGGCAACTGATGTACCTTGATCAGTGGAAAGAGGTACAGATTCTGGAATTACAGAGGGTTGCAGTGGTCCAAGAATAGAAGCCCTTGAATCATCAATGATATCATCAGAACTGTGCACATCAGTAGGTGGATCCTGAGATATATCAGTATGAGATGTATCAGTAGGAGCAACACTTGGTTCATTATTTTGTAAATCAGGACTAGCATCAGACAACCCAATATTAGTTTGAGACATAGCATCATTAGCAGTTGGCACATGAGGATAATTATACGGAGTAAGCAAGTAAGCTGAAAAGTCACTAACAATAGGAAGGACAACATGTGGAAATGGATCAATTAACGGAGAAGTAGAAATATCATTAAAAGGGAATCGATTTTCATGAAACACTACATCTCGAGAGACATAAAAAGATTGTCTTCAAGTCATAGAACCTATATCCCTTCATCCCTGGTGGATAACCAACAAAAACAGCAGTTGAGGACCGAGGGTCAAACTTTGTGCGATGAGCAGACAAGGTAGAGGCAAAACATAGAGAACCAAAGACCCGAAGTGAAGAGTAATCAGGTTCATGCCCATAGAGAGACTGAAAAGGGATATGCCATTGTAAGATGGGTGATAGGGTTCTGTTAATAGGAAACACAGCCGTTAAAATGCATTCTCCCCAAAAATTGATGGCACCCTAGACTGAAATAGAGAGCTCGGGCAACATTGAGGATGTGTTGGTGCTTGCACTCAACAATTGAGTTTTCTTCAAGGCGACCAACACATAAAAACTGATGAACAACCCCTTTAGTCTTAAAAAAATCCGTGAACAGCAATTCGGGTGCATTATCGGAGCGAAAAAACTTTAATATGAGCATGGAATTGATTCTCAGCATAAGCAAAAAATTGAGGCACAACAGCAGCTACATCAGACTTATTCCTCAACAAAAAAATCCAAGTAAAACGGGTACAATCATCAACTATGGTGAGGAAATATCTATAGCCATTATATGTAGGATGTGCATATGGACCCCAAATATTAGCATGTATCAATGCAAAAGGCATATCAGTGATATTATTATGAGAATCAAATGACAATCTTTTCTCTTTTGCTAAAGGGCAAGTAGAACAAAAATGATCCTTGCAGCTAACATCATCAAGATACAAAGACTTTCTCAAAGCATGAATGTGTTTAAAGGATGGATGGCCAAAACGGCTATGCCAAACATCAAAGGATGGTTTATGCACACTAAAAGACATATGGTAAGTGGGAGAGATTGAATCAGTATCACCAGTTGTCGACCCAAGGAAATACAATCCATGACGCACATTACCCTTGCCTAAGAAGCACGGACACGGACATGGACACGACATGGACACGGCGATATGCCAATTTCTAAAAACATAGGACACGGACACGTTGGGGACACATTATGTTTTATTTTTTTAGATAATATATGCATATGAATTGATTATAGTAATACTTTTGCATTAATTAACCACACAAATTGAACAAAACAAATCAAAATATACTTAACATAAAAACAAATCAAAATATATAATTAACCACGACGTTGGTGGCAGCAGAGAGGATGGTAGGGTTTTGTTGATTTGGTGTGGAAGGTAGAAGGAAAGAAAACGTAAAACAAAGAAGAGAACGATTTAGGGTTTTGTCTTCTTTTAAGTTTTCTTTTTGTTTTTCAATTTTTTTATTAAAAAAAATTTGGGCCCAGGACTGTTGGGCCATGTCCCGTGTTCCTCCTCAAGTATCCGGACGTGTCCCTGCGTGTCCGGGGCGTGTCCATAATTAAAAAAAAAAAAATTGGGCACGCCAAATGGTGTGTCAGACACTAGTCCGAAGCGTGTCCAGACGTATCGGTGTCTGATACGCATCCGATACGGACACCCCGCTTAAAATGGAGTGTCCGTGCTTCCTAGGCCCTTGCCAATCGTCCTCAAAGTGAACTTGTCCGGAATAAGGCAACAATCATTAGAAAAAGTAACACATATGATCCATATCCTTGGTCAAAACACTGATCTAAAGCAGATTGTAGCTTAACTGTGGTATATAAAAATCATGTAGAATAACACCATCCATAACCCGAGAAGAACCACTGAACTCCCCTAAAAACCGTAATTGATTTGGCAAACTGATAAACCCAGGCGTAATGCGGGAGATGGATGAAAACATAGACTTTGAGCAACAAATATGAGCAGATGCTCCAGAGTCAAGAATCCATAAGTGCTGGAAAGGTATAAATTCACTCTATTTAACGGTGTTCAGCCATAACATTCGAGGATAAATGAAATGGTCCGCATAAAAGCTGAAAGACATTGTTCGATGCCTAAAGCCTTTTGCTTTCAAGATCATCTCTATAAAATCACAGTCAACGTTACCAAAAGTATTTTCCAAGTCAAGCTTTATAACCACTCCATTTTTCTTCTTTCTAAACCATTAGTCAACTATTTCATTGGCTATAAGGGATGGGTCTTGGATTTGCCTTCCAGCAACAAAAGCTTATTGATATTCTATGATGGTACCGGGCAACACTTTTTTCAACCTTTTGGATAGTACTTTAGTAACGATTTTGTACAAGCAAGTAGTGAGGCTGATGGGTACGTAATCTCCTACCATTCTAGCATCCACCTTTGGAATCAAGCAAGTATAAGTTTCACTAAGGTTAGCATTAATAACAGCATTTTTAAAAAAATCCTAGAACACCTTCAAAGTTCAACATATTGGTCTTGAGGATGTTCCAAGATTTTTTTGAAGAATTCAATCTGGTCCAGGAGATTTGTTCGTGCCCAGAGCTTGTATGACATGCCAAATTTATCTTCTGTGAAGGGGGGCTTCCAAGGCTAGATTTTGTGAAGGAGAGATGGAATCCAAGTCGATGGATGTTGGTAGGAACCTGGCTCCATCTTTTCTGTTAAGGAGATTTTTGTAAAAGCTGATGAATTCGTCCCCAATATCATCTTCATGTAAAAGGTTGGTACCTTGGCTAGATAAGATCTCAGAAATTGTGTTCTTTCAATTTGCAGCCATGTATCGATGGAAGAACCCTGTGTTTTGTTTTTTCTTTTTTAACAAGATACGAACTTTCATTGATGAAATGAAAAGGAACAAAATTGTTCAAGGATACAAACTTTCAAAAGAGTGAAAATACAAAATAAGAGAAAAGAAAAGATTAGAAAGACATAACAAACAAGGAAATCTAAAAACTACAAGAACTACTCTGGACTAATGAAAACACCCCAATTATTAAAAATAACAGAAGGAATAATGAGAAAAGGAATCCGCCAAAGAACACCGATGAGAGGCCTTAAATTTAGATGAATTGAGACAATCGAACAAAGATCTTCGCTTGTGTTGAAAGAGCCTTGTGTTCCGCTCAAACCATAATTCTGAGGAAGCTTTTAACCGCATTAATCCATAACAGCCGAGCCGTAGAAGAGGAGCGTGAATATCCCCCACACAACAACTGCACCAAGTTGTCTTCAGCTTTTAATTGAAAACCCAATCAATCTTGAACTGTCTAAAAAGAGATGACCACTTCTATTTGCGATTTGGCCTTGCATTTCCAACTGCAAAGAATTTTTTCCTCCTTTGCTGTCGGGGCCAAGAATTTTGCTTTAATAGAGGATTGCTTATCAATTTGTTCCTTAGATAAAAAGCCAATTTCCTCTGCAGAATTTATGTGATGAAGCTCACTTTGCTGTGCAACCTTTTATGTCAAGATGCATTCGAAGGTTTCTGTATTCTTTCTAGGTTTTTGTAGCTTTTTTTTTCTTAGAAAAATAACAATATATTTAAAGAACCAACAAAAGGACAACCCAAGGACAGAGGGTAGAGATCATCCTCCCCCAAACTCTAAACAATTGCAGCTTTCCAATCATTGTAGGCTATAATTACAAAAGAATTTCTTGTGGGTTTTTAAGGTTACTTCTCAATAAAACCATGTTCAAGCCATCCTCTTAAGGGGGTTGTTTTACCACCATTTTTCTACAAGGGGAGAAATTAATGAATGTTGGAGTCAGGCTATCTCTTAAAACTTGATTTTGAGAAGGCTTATAATAAGGTCGATTGGTCTTTCTTATATGTAGCACTTCAGGCGAAAGGCTTTGGTGTCAGATGGCGAATGTGGATTTGGGGCTGTCTCTTGACCACAAACTTCTCTGTTATTGTAAACGGAAGACCTAGAGGGAAGATTTTGGCAAAGAGGGGTCTTAGATGGGGAGATCCTCTCTCTCCCTTTCTTTTCACCATAGTGGGAGATGCCTTCAGTAGGTTAGTTCATTTTTTCTACGAATGGAACATCCTAAAAGGTATCACTGTAGGCCAAAATTCTATAGAGGTAACACACCTTCAGTATGCAGACGATGTACTGATTTTTAGCCCTTATTCACCTAAGATGATGGAAAATTGGTGGGGTCTGATTCAAATTTTCCTTTCTATGATGGGGCTTTCATTGAATGTTGCTAAAACAGCAGTGATAGGAATTAATTGCAGTGAGGCAGATGTGAATTTCTGGGCCTCTATTTTCAGGTGTAAGGCAGAATTCTTTTCCTTCTCCTATCTTGGTTTCCCTCTGGGAGGAAATCATAGAATGCCAGGGAAGCAGTGGTGGACAAAATGCACAAGAGACTAGGAAAATGGAGATCTTTGTTGCTATCAAAGGGGGGTCGATTAACTCTAGCACAAGCAATTTTGAACAGCATCCTGATATACTTTTTTTCAATCCTAAAAGCTCCTAAGTCGGTGATTTCGACCATGGAGAATCTCATGCGAGACTTCATTTGGAATGGTGGAAGACATAGACCAATTGGAAATCTTGTGAAATGGTCATGGACTGCCTTGCCTCACAAACAGGGGGGTCTCAGTATTGGTTCCCTTGGATTAAGGAATAAAGCTCTTCTCATGAAATGGCTTTGGCGCTTTGCAATGGAAAAAAATAGTTTTTGGAGGAAGGTGATAGCTTCCATTTATGGGGAGGATTCACATGGTTGGATGCCTTTGCCTTTACAAGGGTCTTCTATTGGAAGACCTTGGATAGATATACAGAACGACCTAGAGGCCTTCACAAAGCTCACTGTTTTTAAAGTTTCAAGGGGCAGTGAGATTTTGGGAGGATAGATGGATTGATCCTTCCCCCTTTTCTTCTTTGTTCCCTGATTTGTACAGCCTTTCCACTAAAAAGAACCATTATGTGGTAGCTTGCTGGGTTACCGAGGCTTCAACTTGGGATATCGGGTTGAGAAGGAATCTTTTTGATAGGGAGATCGATAGTTGGGCGTCTTTCATTGATAAATTAGAAGGTATGGTTCCGGGCCAAGGCAAGGATCGATTATGGTGGAAGGCAGATAGTAAAGGTTGTTTCACAGCGAAATCAGCTTTTATAGCTTTGACGGCTCCTTCCCCTAAATTGAACACGACCACAACTTCTATGATTTGGAATTTTAGGGTTCTAAAAAAGGTGAAAATTTTCCTATGGGACTCTCTTTTATAGGAGCATAAACACAGCCAAGAGAATACAAAGAAAGCTCCCCCATTGGATGCTCTCCACTTCGGTTTGTGGTCTCTGCCTAAGAAATTCAGACTCGGTGGATCATATTTTCATCCACTGTTATTTTGCCACTCGGGCTTGGAAGTATATGGCTTCACTTTTGGATTTTTCCTTCTGTCTTCCTAAACATATAGATGACTTCATGGTGGAAGGATTTGGTGGAGTGACGCTCAAAGGCAAAGCTGCGGTCCTATGGCATTGTGCAGCGAGGGCTCTTCTTTGGCATCTTTGGAAAGAGAGAAATAGTAGACTTTTTGAGGACAAATCAATCCCTTTAGATTCTTTTTGTGTGAATACAAAGCATACAACTTCTTGGTGGGCCACAATCCACAAGAAGGCTTTTGGAAACTACCGTCTTTTTTTGATTTTACAGGATTGGAAGAATGCGATTGTATAGTTCTCTTTGGGGAGGGTTCTCTCTACCTTCTGCCTTTAGGTTGTTCTTGCCTTTTTGGGCTCTTTTGTTTTGAATGCATTCTCTTTGTTTCTTATAAAAAAAAAATGTTGGAGTGCATACTTTCAAATTTTTTGGGGGGGGGGGGGGGATAGGATAATGGTCCGAGGTTGTCCTATCCAGCTTTTTTTAACTCATGCTAAAGAGAATTTGGTGATGATGGACTTTGATAGAAGGAAACGATCAATGAGGATGAGTGTAGGCAAGTATCTGAAATCAGACCAAGTTAACCTACCATTGGAAAGAGCTACATCTATGAGCCCAACCTGACTGATGGAGGTGTTGAACTTTCCCATAAACTTGGAGTGTCTTTTGCTTGAAGTATCTATTCTTGAATATTATAGGTTATCACTTATCAAAGTTGTTTAACTTTGTTTTACTAAAGTTTTTTCGATTATTTTATTTCATGGACATATCTGAGGATAAATAGTCTATCCGTCATTCTACAATACTATTATTTCTTGTATGTAAAAGAGGATGAATATTAAATTTAGTGAAATAGTGTCAATTTAATACAACTGTTATCTAATTTTTTTTTATACAACCGTTTTCCTTTATGAGTTACATGTGATTGAATGCACGGTACGGTTGAATTGTTTTGAGAACATCTGTTTGCATTCACATGTAAACATTCCTAATGTGTAGGTAGACTTCTTAAATGACATACATCTATTTTCTTCTTTTAAACCATATTTTTATATCTTCAGGTGGTAATGGCCTTACAATGTCGTGGAGAGCATGTGCTTGGAGGTCTTCATTCTGGAATTAACTCAAGACATATCTTTCTGACTAGCAAGATGGTAATTGTTTTCTTACAAAGAAAAGCTTATTAAAACTCGAGTTATTTTTTTTTTAAATTATTCTCGTCATATATATTTTTTTAACGTACAAAGTTAAACGTTCCATAGGGCAGGATATTTTCTGTTGCAAGTTGTGTGTGGCTCTAGGTTGAACTTGAAAGCTAGACAGTTTTGTTCTCGCTTCTAGCTCCTCTTTGAAAGACTTGGAAAGAGACTAAGGGGCAAAGTTTTTGAGGATGGGAAGAGTGTTTCTTTCTTTCGTTTATTTTTTTGGGGGGTGGTTGAGAGAGGTTTAATGTTTTGAACTAGCTATCGATGTCCAAAGTCCTATTTATTTTTTATTTTTCTCATGACTATTGTTTCTTTTTAAAATCTGTTAGTTAGATCTGTTTTTGGTTTTTTTTTTCTTGGGGTTTAGTGTACTTCAGATTTTGAACTAACGACAGATTTCTGGTTCTAGTTCTCTCTTCGCCTAGTTCACTTTCAATTTTTCTATTCAAGTTTCAACATCCATATTTCTTATTAATAAAGGCTACTTACAGACAGACTTATTCCTTAGGTAGAAGAATATACTGGCTTGAATGTACAACCGCACAAGGCTATTGTTGGAGCTAATGCTTTTGCTCATGAAAGTGGCATCCACCAGGTTAGACTTGTTAGATCTTGATATGCTAGGTTTTGTTGTTCATGGCTAACCATTTATGAAGTGTTTTCATATTTATCTTTCAAAATCCAGTGTGATGTGAAAGCTTCCAAATAAATTCTCATAGCTGACTATGTCAGGGTTCTAGCATAAGTGTTTATTGCTTTAAACACATTTTAAAGAAAACAGTTTGCCATTTTTTCCTTTATTTCCCATCCCCCCTGTGAGTTAATTGAAGAAAATTTCTTTTTTAGATTGTCAAATTCAGGAAACAGAGATACAGAAAGGACAGTAAGGGAAGATGATTCTTCCCCTATGAGGTGATGGAGATTACATTCGATTCTCCTCAAATTGGGAGAAAGATAAAAGAGTGTGAAACACCTTCTAAAGCACTCCAAGAGAAAGGAAGAAAACAGTGTCCTCCTATATAGTGTTAAAATTTCTATCCAATCTGTAAGAAAATTTTCCTATTCCTTTCAAGCTGAGCGCTCTATATGCTTGTAGTCTTTTGCTTGAAAGGGTGGCATAGCACATAAGGCCGATCCTCTTCATCCAAAGCCTTGTTGAATACAAGACGCAGCTGGTTGTATCTTGTAGTTTCATCCTGGCCTCTGTTATTATTGTACTTGAGTCATCTGGCCGTAGAATGTATTTAATTGTTCATAAAGGACCAATTGTTCATTACTAATTACTGTCTGAGGAGTTACATGATTGTTATACAGCGGCTGCGTTCTCTTATAAACATGGAGGACTCTTTTAATTATTTTGATGCATAGTCTTTGCCTTAAAGTTCATAATGAAAATATTGATTGGCTCTTATTCTACGTTTTTAATAAATATTACTATTTTTGTGTGAGCCATGTGGGTGACTGTTTATCCTGTTTTGTTAGGCTCAAATTGTATGCTAAATATTGCTTTTGTTACACAGGATGGGATGCTTAAGCACAAAGGTACTTATGAAATCATATCCCCTGAAGACATTGGCTACGAACGATCCAATGATGCTGGTATTGTTCTAGGAAAACTTAGGTACACCGTGTTGCTTTGTGTAAAATTACAATATTGCTTTTACCTTGACAAAATCTCCCTACCTGCCTCCCCTTTCCCATCTCTTTATGCGTGTGTAAGCATGTATGTGCAAGAAGAAATGTTTAGTGTGTTTGTTCGCTGGGATTTATTTTGATGGTTGGCTGGGATTTGTTTTAAGGTTATTTCGGGAGTTGTTTATTTCGAGATTTGTTGGGCTTTTGTTTTTGTATGCCTGTGTTTTTGTTCTCTTTTTCTTTTATCTTAATGAAAAGTGCAGTCTCTTATTAAAAAAAAAAAAAATTAGGTGTTCCTCATGAGCTGCAGAAAAAAGAAGGAAGAGATTGTTTAAAAATGCTATGAAGCAAAGTATCATGGGTGGCTTATTGGTCCAAAGGGGATAGGGTAAATTGAGAGGGAATGGGTTCAAACCCCATGATAACCACCTACCTTAGGAATTAACTCTTATGTATTTCCTGGCACCAAGTAGGGTCAGAGGCGGCTGTCCCGTGAGATTAGTCGAGGTGCATGTAAGCTGGTCCGGACACTCACGAATATAAAAAAAAGCAATGAAGCAACTTTTTTCTTTACAATTGAAAATACCCAAAAAATCGTGCCGTGTACCTTACTTAGTGCACCGGATCCCTAACAGTGGGTGCCGTGAAGCTAATTATGCCTTTTAAAGTTTGGTATTGATACATGTACAGACATACTAGTGCATTTCTTTTCTCTCTTAGCTTTCTAAGTGTATATATTTTGGTTTGGTACCTTCTTAAATAGTTTAGCATCTATGATTTATTTAATAACTAATATTTTAGTGTGTATAAAAATTATTTTCACCATTCTCTCTTAAGAAAGGTTTCCTTAATATATGTAGTGTGTTTCTGTGACATTTCAAGTGTCTGAGGTGTAAATTTCGGACAGTTTCCCAACTAGAGTTTTTGTGCTTTCTGGAGGGCAAGTATCTCAACTAAAAGGATTAGAATCTAGTAGGCTGGGGACCATGATCAAAGGAAAATCTAGCAAGAGGCCAGCACTGGGAGAACTTTCTGATTCTACTCATTCAAGTCCATTAACACAGAGATCCTGCAACAGAGTAGATTGGGGATTCTCATCAATGGTTTCCCTCTATTTTTAGTATCTCTGCTTCTCCCTCTATCTTTGTTTGGGATGCTTGGGATAGGAGGTCTTCTTCTTGGAATATAGTAACAAAAGGTCGCTGAAAGATGAAGAGATTACAGTTTTGTCAGAGTTATTATCTTATATTTCTTTGACTGTTCTTTCGGAGGAAGGTGATTCTCGTTGTTGGAGTTTGGAAAATTCAGGACTCTTTTCAGTAAATTCTCTATGTAAGCTTGGCAGGGCTGATTTGCATCTTTCAAAAGCCTTTTTCTCCTCTCTTTGGAGTCCGGGCAGTCCTAAATGGGTGAATTTGTTAGTTTGGTTTCTTCTATATGGTAAGGTTAATACTTCCGAGGTTCTCCAACGGAAGTCTCCTAAAATGGCCTTGCAGCCATCCATATGTTTGATGTGTGCTGAACAAGGTGAGCTTTTGAGTCACGTTTTCTTCTTCTGTCCATTTGCTTTAGCTCGTTGGAGTTTCTTCTTCCAGTTCAATTTGGCTTGGGTTTGGGATCGTGAGGCTACAAGCAACATCATTCAGATCCTAAGTGGAGCTAATTTTCCATCTAAATCTAACCTTCTTTGGATTAATGGTTTCAAGGCAATTATTTTAGAATTATGGTTCGAGAGGAACCAAAAGGTTTTTGAAGATTCTGGTCATTCATCCTTGGAATGTTTTAATCTCGCCAAATTCAAAGCATATCAATAGTGTGCTCTTTCCGATCTTTTCTCTTCCTACCCTCCTAGTATGATTTAGTATGAATTTGGGGGCATTTATAACTCCTTTCTAGTTTGATGTTATTTTTGTTTTCTCTTTTTCACTCCTTTCAGAGCTTGTATCATTGAACAATATTGTTCCTTTCCATCTCTGGTTGAAAAGTTCGTATCTTGTAAAAAAAAAACATTGGTGGTATGTTTAATTACTTAAGAATAAGTACAGAGAAGAGGAGGTTGAAGAAGTTATCATGCGCGAACGTGCATAATTTGTAAGGCATTTTCATAAATTCGAGGTCAGATTAATTTCAAGAATATTTGAATACAAATGGAAAAATTGGCCCCTTTTAGCTGTAAAATATCCTATAGTTGGGTACAAACAATGATGCAGCTGTACCTAGGGAGCTGAAGTAGTTTATCTGGGACAGGAGAAATGAAAACATCTGTAGCCATTCTATCGTGGCGTTAAAACTTGAAATTATTTTGTTACAACATTAACAGGATTAACTTCCTTTTGTTTATAGTTTCACTCCTTGTATCTGGTCCGAAATATACCACCCGCTAACATGGTTCATTTTGACAATTTTTCCAGTGGACGCCATGCCCTTAAAAGTCGACTTCAAGAGGTAAATTGGAATCTTCAAGCTCAATGGTTATGTCTTCCTTTCTGGCAATCTGTTAACGTCATATCTCATTTTTTCAGCTTGGTTATGAGCTTGACAATGAGAATCTTGACAACATATTCTGGCGTTTCAAAGCAGTAGCTGAACAAAAGAAGGTAACTTGAAATTGTGTCCCTCTCTGTTATGCTGAGCTATTCCCTCCCCTGCCTCTGCACATATTAGTAAATACCAAAAAGGATTCTACTGCCTCTGCACATATTAGTAAATACCAAAAAGGATTCTACTTTGGGTGGAATCATACCAATGAATGCATGATCCCATCAGAACTCTATAGTTAAGCGTGTTTAGACGAGAGTAGTACTAAATTAGGTGTCTACTTGGGAAGTCTATGTGTTGCACTCCTTTTAAACTTGTAAAAAAAAGAAAAAAATCAGATTTTTTTATCTAAATTCTTTTGCTCTAATTTATCTGACATTCCAGAGAGTAACAGATGCTGATCTTCGAGCATTGGTATCCGATGAAGTTTTTCAACCAAGTGTTGTCTGGAAGCTGCTAGATTTGCAGGTTTAAATTTTGTAGACCATTATATTTGGGAAGTTGTTTGGACCTACTTTTAAATACACGTATTGCATCATTTTGACAATACCTCAAATGCAGGTAACCTGTGGAACTCTTGGTCTTTCTACGGCTACCGTTAAACTCCTCAATGCTGATGGTAAAGAGCATACTAATTGTGCTGTTGGAACGGGGCCTGTAGATTCAGCTTACAAGGCTGTAGATGGCATTGTGAAGGTTTGTATAGGAGAAACTGCTATCTAACTTTTTATATTTTCCATTACATCTGGAATGTACTTTCAGGAAGATTTCCTGGTATAGTCAAACTGGGATATCTTAGATAATTTGTTTTATGTTAAATTACATGAATTTTTGGGTTCTGTCTAATAAGTCCCTGAATATTAAAAGTGTTTAATAGGTAATAGGTTTTTGAACTTTCAATTTTGTGTCGTATTCAACATTTTTTAATTTTGTGTCTTATTCAACATTTTTTATAAAAATTTCATGGACGTATTAGACACAAAGTTGGAAATTCAAAGCCTTATTCGATGCAAAATTTGAAGACTGAACTTAATTTAACCTAAGTTATAACATAAAGTCGTCATGATCGATAAACCAGGCTTTCATGTCCTTTGCATGCATGTCTAGTTCTGTGCTATTCTGAATGATTAACTATGATGTTGTCTTCTTGCTTTAAATTCCTTGCTGGTGGTCAGGTGTGTCTTCCTTGAGTGAATCTGACTGTAGAAGAGTTATCAATTTTTTTAATGCAGGAACCAGTTGCACTTCTTGAGTATTCGATGAACGCTGTCACTGAAGGCATTGATGCAATTGCCACGACCCGTGTTGTAATAAGAGGGGAAAACAGTTACACATCTACTAATGCAATTACAGGAGAAGCAGTTCAACGAACATTTAGGTAAACTAGCTACAAGATAATGTGATACCTGATTATTTTCTTTTGAAGGAAAATTTGCAAAAACCACCCCAGGATTTTAGGAGTTGTTACAATCATACTTTTGCAGTAGCAGTCCGATTACGTGCCCCCTAAACTTTTTTTGGAAACGAACAATTTCATTGATATAATGACAAAAGGAGGGAGAAATGATTACAAAAAAGAATGAGAACCCACAAGCGGGTTACAAAAAAGCTCTCCAATTTGTCAATAAGGAGGCAAAGCTATACGAGTGGAAGAGGGGGGAGCATTTACACCGACCAAAAAAACAATGGATTCAAAAGTGGCATGTGGTGTGCGCTCCAAATCTTGGAAGATTCGACGGTTCCGTTCATTCCATGTAGTCCAAAGAAGAGCCCGAATGAAGTGGATCCAAAGGCTGGCTTTTTCTTTCTTGAATGGGTGGCCCCACAAGAGTCGAAGCAAGCAGTTGAGACATATTGGTGGGGAGGGCGGCCGACCATCCAAAGAGTGGTAAGAGTTTGTTCCAAAGAGTTGCTGTGTAGGGGCATGTGAGGAATACATGTCCGATGGACTCACTGTGTTGCTTGCATAGAGGGCACCATTGTGGAGATAAGACCATATATGGCATGCGTCGTTGTAGTTTGTCGTGAGTATTCAATGCTTGGTGGCTAGCTTCCCATAGGAAGAAAATCTTCTTTGGGTAGGATTCCTTCCAAATTGCTCTATAAAGATCCGGTGGAGTAGATGATGTGTTGATGCTTTGGAGGTCTTTGATGAGGGAGCTGGTAGTGAAGGATCCATGGGATTCCGGTCTCCAAGCAATGGAGTCCCTATTCATAGATGGTTGCTTTGAGGAAAGGCAGTGTGAAAGGGCAGCCAACTCAACAATCTCCGCGTCCTTGAGGTTTCTGCGAAAGCGTAAATTCCAAGATTTGGTTTCAAGAGACCAAAAGTCCCGAACATGGGCATGTTTCCTGGTATGAAGAGCAAAAAGGAGGGGGTAAGTTCGACATAAAGGTTCTTCATTGACCCATGTGTCTTCCCAAAACAGAGGGAGAGAGCCATCTCCCAACCGGTAATCACAAGATTAGAGATTTATGCTTGGAAATGTCTCTCCAAGGACCCGCGGTTGGGATTCTATCCAAGATTAGAGATTTATGCTTGGAAATGTCTCTCCAAGGACCCCTAGCAGCAGCGAGAGAGCGGGAGTGTAGGGCATTGTGGGTATCATAAATGGAGTATTTTGCTTGTATCAACCGCCTCCATAGAGCTGTTTCCTCTGTAAGGTATCTCCAATTCCATTTAGCTAAGAGGGTTGTGTTTTTGGCTTTGAAGCTTATGATTCCAGGACCACCAAAGTCCAAAGGCTGCGGTACAATATCCCATCTAACAAGATTTTGTCCCCGCTTGTCCAATGTCCCCTTCCAAAGAAAATTCCGAACGGTCCTATCAAGCTCAATGGCAACCTCGAGGGCATCAAGAAAAGAGAGAGGAAGTATATGGGGAGATTGGAGAGTGTGGCATTGATAAGGGTGAGTCTACCTCCCTTGGATATATTTGTATGCTCCCATGAGGAAAGTCTTTTTTTGATTTTCTCCTAGACGGGATCCCAAAAGGATGGGGATCGAGGATTGCCATTAAGAGGGAACCCGAGGTAGGTGTTGGGCCAAGACCCTTTTCTGCAGTGAAATCTGTTGGCCAAATATTCTAAAACACTATCTTCCAAGTTGATGCCCAATATCTCTGTTTTTTGTAGGTTGGAGTTCAATCCAGAAAACCTTGATAAAATTGAATAAATTCTTGAGTTTGTTGTCATTCAAAGTAGAGAAGAGGAGGGTATCATCAGCAAATTGAAGATGCGTGACTCTAATGGGAGGACTGCCAACCTCAAAGCCTTCAATTGAAAGCTGCTGCTTGGGACAAAATCCTGCTAAAATAATTAACCACAAGGATGAAAAGGAAGGGTGAGAGAGGGTCACCTTGCCTTAAGCCCTAGAAGCCTTGATTTTGCCCTTAGGTCGCCCGTTTATGATGATAGAGAAGTTTGCCGAAGAGATGCAGCCTCTAATCCATTTTCTCCAAGTTGTACTGAAGCCTTTAGCCAAAAGAATACCGTCGAGGAAGTCCCAATCTACCTTGTCAAAAGCTTTTTCAATATCAAGCTTGATGACAACCCCAATATCCTTTTTCCTAGTCCATGTGTCGATGATCTCATTAGCAATCAAGGAGGCATCCAGAATCTGTCTGTTGTCTACAAAAGCCGATTGTGAGGCAGAAATTGTGTGTGGAAGGACCCTCTTAAGCCTCTCAGAAAGGCCTCTTGCAATGATCTTGTACATACATGAAGTCAAACTAATTGGCGGTAGTCACTAACTGTTCTTGCATCAACCTTTTTTGTTAAGACTCGCATTAATGATGCCATTCTTGAAAAAATCTTGGAACACTCTCATAATAACAGGTTTGAGTATGTTCTAATATTTTTTAAAGAATTTGGCAATGAATCCGTTGGGTCCAGGGGTTTTATTGGTGCCTAGGTCCTTAATAGCTTGCCAAACCTCTGTCTCAAGGAAGGGTGCTTCGAGGAGGGAAGATTGTTGCTGTGTAATGGTGTCCCAGTTGATAGGATGTGGCAGGAATCTAGTGCCCTCTTTTTTGGAATACAAAGATAGGAACTCTAGCTCAATCTCTGTGTCATTAACCAAGCTTTTGCCATCCCGAGATAGGAGCTCAATAATGGAGCTTTTCCTGCGTCTGGCAGCCACCACTCTATGAAAGAAGCCAGTGTTCATATCACCCTCTTTGAGCCACCTTAACTTACATCGTTGTCTTCAATGATCTTCTTCCATTATGGCTAATGAGTGAAGCTGTTCTTTTAGAGAGACCCTCTGTGAAATTTGCACCGATGGGATGGATCCCTCCTCTTCTTGGGTGTCAAGGATAGCTAGTTCCGCCAGCAGCTGATCCTTGTGGCGGGTCACCTAAACTTATAAAATTGTTGGAATTAAACCCTTAAATATAACAAAATGCACAAACCATTTATAGATTGTTACCAACCCCTAGTTGATGGGTGATTTTTGCAATTTTCCCTTTTTTTTAAATACAATCAATCAGAGTACTCTTGTTTGAAGTATTATTCCCTCTACAATATTACATCTAAACTTTGTTAATATTCAACTCGAAAATATTTGGGAGGGAGAATTTCGAAAGCTATATTAATCAATGGTGCAACCCAGTTGGCAAAGGGCTTGTAGTCTCTCGGTCATACTGGCTTAGAGGCCTCAAGTTCGAACCTTCTTGTTTCAAAAAAAAAAAAAAAAAGTTCGAACTTTCTGGTGAGCTTAATACCAAAAGCTCTTGATATCTCTCGAGTCCAGGCCTTGGCGGGCACGGGTGCCCCTGGTACATGAGAGCAAAGCTCCGACTCCAAGTTATTCAAATAAAAAAAGAAATCAACCCATATCAATAACTTCAAAGATAAATCGATTAACTTTTTCGTATTCTTTGCTACACACAAGCAATCTAGCATATCGATAATTGTCTGTCTAGTGATATGTTTTTATCGTTGCAGTGGTATCGGGGCTGGCATGGACATAGTCGTATCAAGTGTGAAGGCCTACATTGGTGCACTAAACAAGATGCTTGGTTTTCAAGGAGTTGACATTAAGGTGACCGAAAAAAGAACATTGTCTGCATAAAAATTGCTTCAAGAATTTTGCTCATGGAAATTTCGACTAAGTTCCAGTATCTTATGTCGTTTGTAATTTAGTTGTTTGTGTTATTTGAAACCTTTTCAGAAGCACCTGCTTTAATGTTTTAGTTTGTAAACAGTGAACTGGCTTTAATTAGTCATTTTATGAACGAACCTTTTTTTTTTTTAATTAGTATTGAATGTAAGTTATCTAGCAAATGATAAAATTTTGGTATTTTTTATATGCATTCAAAACTTAAAAGTTTGTTTTAGTTAATTTTTCTGTTGGTTTTGAAATGCTGTCTTATGAGGGCATTGACCACTGAACCAGCCAAATAGGTAAAAACCTTTTGGTCTAGAACATATGTCTTTGTTCTATGTCTTAACTAGTTGAGTTGTACTCAGGTTGCTTCATTTAGAGAGATGATACAATTATATTATTGTCAAAATTAATTAAAAAATGCATAATTTTAATGTCTAAATATATGTTTTACTTTTACTTAAAGGTTTAGCGGGTGCAACTTCTTTGCCAACGATCGAGGGGGTTCAACTTCTTTGCCAACAATCTAGGGGGTGCAACGTCTTCGCCAACGATTTGAGTGGGTGCAGCTTCTCTGCAAATGATTGAGCGGGTGCAGCGTCTTCGCCAAGGAGCGAGTTCAACTTCTTCGCCAACGATCGAGGGAGTTCAACTTATTGAATTAGCTAACTTTAGAATTTGATTTGTCATTCTCATTTCTTTTGGACATGAATATTATATGTCAAAAATTTTTCTATGATTTAGGGTTGGATTTGGAATTTTTATCATTTAATCAATCCATCATTGAGCAAAATTTTAGCAAAATCACGATCCTGATGGGGCCAAAATTTGTAACCATAATTAGCCAAATTTTGAATAAGGAAGAATCTTTAAAATTTAATTAAGGTTCATGCAAAATCCGAAGCAAAATATCCTCCTTCCCAAAATTGATTTCAAACAACCCTACTCTAAAATTTCATATATATTTGTATGAGGCTATGATACTAAAGCAAAATATGGAGAAAAAGTTTGAGCGTCTCAATCTTCATGGTTCTGTAGCGTTGAAAATCTATGGGTATTCATCGATCTGGATTCCCACGGGTCCAGAAAGTTTTGCCAATATATATTTTTTTTCTTCTGCAGGTTTCAGGCATAAAGGTCACGTTTGAAGATTGCATAAGGCGATGGTACCTTCACCGGCGGGTAGCTTGCTTCTTGTTCATATGTGCATCAATCGGGTCATCAGAACTTGACAGAACCATCGATGGCTGAAGCAATTTTTTTTTTCATATCTAAAATGGCCCCATGGATCTGCTATGCTGCCGCGGGTACTCGGGTTGCAGAGTGACGTCATGCCGCCATGGATGAATGGATTTGGTTGAACTCTGAAGGAAGAAGATGATACATGATTTTTTTTCTTTTTTAATTTGTTCGACCAGAAATTCAATAAGAAAAAACTTAAATTTATGAACTTATATTTGTATTTATGTATGTGAGTGTTACAACCTCTAATGTATAATCCTCTGATTAATTCTCTCAAATGTAAAATTTAATTCAAGGGTTCGTGAGCTTGTTCTCGAATCAATGAGTTTGCATCTTTAGCTTCTTGCTTGACGAAGGATCTTTGAAGTCTTTAGCTTTGAGCTTGTTTGAAGACTTTGACTTGTTGATGAAGTTCTTTGACTTGTAGCTTGATGAAGACTTTGTGAAGTCTTCAGCTTTGCGCTCGTGAAGTTCTTCGGTTTTGCGTTAGTAAAGTTGTTTGGCTTTGTAGCTTGTTGAAGTTCTTCGGCTTGTAGCTTGTGAAGTTCTTCAGCTTTGCAGATTGTGAAGTGTTCTGCTTCGGAG

mRNA sequence

GGCTCCATAGAAAGGAAAGAAATTAAGTAAAATTTGGCAACCCTATATTAGCTTAGCCGCCGTGAATGCGAAAGAGGAGGAGAAAATTTCTTCTGTGAGTTCTTTTTCCTGCTTTCTCTCGGCGAGGTTGCTGTTGTTGTGTTTAAGATGGCCATGGCGACAGCATTTTCAAGCCCGAATCACTGTTTTGGTCGGCAATGGAACTCGCAATTTCCTACCAGCGGTCACATTTATCGTCGGAACTTGCAGAATCCGTCGTCATTTAGGTCGCCGGCGGGCCGTGTTTGTTGTAGTGAGGCACAGAGCATAAGCATAGCTTCCGATTCCAAACCGAATTCATCTGTTCAACGCCCGCCGTACATCCCCAATCGCATCCCTGACCCTTCCTATGTTCGCATTTTTGATACGACTCTCCGCGATGGCGAGCAGTCCCCAGGTGCGTCCCTCACCTCTAAAGAGAAGCTGGATATTGCTCGTCAGCTCGCCAAGCTCGGCGTGGACATAATTGAGGCTGGATTCCCAGCTGCTTCGAAGGAAGACTTTGAGGCTGTGAAGATCATTGCCAAAGAGGTGGGAAATGTTGTCGACCAGGACGGGTACGTGCCTGTAATTTGTGGTCTCTCGAGGTGCAATGAGAAGGATATTCGAACTGCATGGGAGGCCGTGAAGTACGCTAAGAGACCGCGGATACATACGTTTATTGCAACTAGCGAAATTCATATGAAGTATAAGCTGAGGAAGACGAGGGAGGAAGTGATTGAGATTGCTCGAAGTATGGTGCGGTTTGCACGAAGTTTGGGCTGTGATGATGTTGAGTTCAGCCCCGAGGATGCTGGGAGATCTGATAGGGAGTTTCTTTACGAAATTCTGGGTGAAGTTATAAAGGCTGGGGCAACTACTCTCAACATACCAGATACTGTGGGTTATACCATGCCGGTTGAATTTGGGGCGTTGATTGCTGATATAAAAACTAATACGCCTGGCATTGAAAATGTTATCATTTCGACACACTGCCAGAATGATCTTGGACTCGCAACTGCCAACACAGTAGCAGGAGCATGTGCAGGTGCAAGGCAAGTTGAGGTCACAATTAATGGCATTGGTGAGAGAGCTGGAAATGCTTCGCTGGAGGAGGCAAGTTATTTATTTAATTTATTTCCTACTTATTCTTTCATTTATTTTGAAATAATAATCATTCAAGAAGGTTGTGTGGTAATGGCCTTACAATGTCGTGGAGAGCATGTGCTTGGAGGTCTTCATTCTGGAATTAACTCAAGACATATCTTTCTGACTAGCAAGATGGTAGAAGAATATACTGGCTTGAATGTACAACCGCACAAGGCTATTGTTGGAGCTAATGCTTTTGCTCATGAAAGTGGCATCCACCAGGATGGGATGCTTAAGCACAAAGGTACTTATGAAATCATATCCCCTGAAGACATTGGCTACGAACGATCCAATGATGCTGGTATTGTTCTAGGAAAACTTAGTGGACGCCATGCCCTTAAAAGTCGACTTCAAGAGCTTGGTTATGAGCTTGACAATGAGAATCTTGACAACATATTCTGGCGTTTCAAAGCAGTAGCTGAACAAAAGAAGAGAGTAACAGATGCTGATCTTCGAGCATTGGTATCCGATGAAGTTTTTCAACCAAGTGTTGTCTGGAAGCTGCTAGATTTGCAGGTAACCTGTGGAACTCTTGGTCTTTCTACGGCTACCGTTAAACTCCTCAATGCTGATGGTAAAGAGCATACTAATTGTGCTGTTGGAACGGGGCCTGTAGATTCAGCTTACAAGGCTGTAGATGGCATTGTGAAGGAACCAGTTGCACTTCTTGAGTATTCGATGAACGCTGTCACTGAAGGCATTGATGCAATTGCCACGACCCGTGTTGTAATAAGAGGGGAAAACAGTTACACATCTACTAATGCAATTACAGGAGAAGCAGTTCAACGAACATTTAGTGGTATCGGGGCTGGCATGGACATAGTCGTATCAAGTGTGAAGGCCTACATTGGTGCACTAAACAAGATGCTTGGTTTTCAAGGAGTTGACATTAAGGTTTAGCGGGTGCAACTTCTTTGCCAACGATCGAGGGGGTTCAACTTCTTTGCCAACAATCTAGGGGGTGCAACGTCTTCGCCAACGATTTGAGTGGGTGCAGCTTCTCTGCAAATGATTGAGCGGGTGCAGCGTCTTCGCCAAGGAGCGAGTTCAACTTCTTCGCCAACGATCGAGGGAGTTCAACTTATTGAATTAGCTAACTTTAGAATTTGATTTGTCATTCTCATTTCTTTTGGACATGAATATTATATGTCAAAAATTTTTCTATGATTTAGGGTTGGATTTGGAATTTTTATCATTTAATCAATCCATCATTGAGCAAAATTTTAGCAAAATCACGATCCTGATGGGGCCAAAATTTGTAACCATAATTAGCCAAATTTTGAATAAGGAAGAATCTTTAAAATTTAATTAAGGTTCATGCAAAATCCGAAGCAAAATATCCTCCTTCCCAAAATTGATTTCAAACAACCCTACTCTAAAATTTCATATATATTTGTATGAGGCTATGATACTAAAGCAAAATATGGAGAAAAAGTTTGAGCGTCTCAATCTTCATGGTTCTGTAGCGTTGAAAATCTATGGGTATTCATCGATCTGGATTCCCACGGGTCCAGAAAGTTTTGCCAATATATATTTTTTTTCTTCTGCAGGTTTCAGGCATAAAGGTCACGTTTGAAGATTGCATAAGGCGATGGTACCTTCACCGGCGGGTAGCTTGCTTCTTGTTCATATGTGCATCAATCGGGTCATCAGAACTTGACAGAACCATCGATGGCTGAAGCAATTTTTTTTTTCATATCTAAAATGGCCCCATGGATCTGCTATGCTGCCGCGGGTACTCGGGTTGCAGAGTGACGTCATGCCGCCATGGATGAATGGATTTGGTTGAACTCTGAAGGAAGAAGATGATACATGATTTTTTTTCTTTTTTAATTTGTTCGACCAGAAATTCAATAAGAAAAAACTTAAATTTATGAACTTATATTTGTATTTATGTATGTGAGTGTTACAACCTCTAATGTATAATCCTCTGATTAATTCTCTCAAATGTAAAATTTAATTCAAGGGTTCGTGAGCTTGTTCTCGAATCAATGAGTTTGCATCTTTAGCTTCTTGCTTGACGAAGGATCTTTGAAGTCTTTAGCTTTGAGCTTGTTTGAAGACTTTGACTTGTTGATGAAGTTCTTTGACTTGTAGCTTGATGAAGACTTTGTGAAGTCTTCAGCTTTGCGCTCGTGAAGTTCTTCGGTTTTGCGTTAGTAAAGTTGTTTGGCTTTGTAGCTTGTTGAAGTTCTTCGGCTTGTAGCTTGTGAAGTTCTTCAGCTTTGCAGATTGTGAAGTGTTCTGCTTCGGAG

Coding sequence (CDS)

ATGGCCATGGCGACAGCATTTTCAAGCCCGAATCACTGTTTTGGTCGGCAATGGAACTCGCAATTTCCTACCAGCGGTCACATTTATCGTCGGAACTTGCAGAATCCGTCGTCATTTAGGTCGCCGGCGGGCCGTGTTTGTTGTAGTGAGGCACAGAGCATAAGCATAGCTTCCGATTCCAAACCGAATTCATCTGTTCAACGCCCGCCGTACATCCCCAATCGCATCCCTGACCCTTCCTATGTTCGCATTTTTGATACGACTCTCCGCGATGGCGAGCAGTCCCCAGGTGCGTCCCTCACCTCTAAAGAGAAGCTGGATATTGCTCGTCAGCTCGCCAAGCTCGGCGTGGACATAATTGAGGCTGGATTCCCAGCTGCTTCGAAGGAAGACTTTGAGGCTGTGAAGATCATTGCCAAAGAGGTGGGAAATGTTGTCGACCAGGACGGGTACGTGCCTGTAATTTGTGGTCTCTCGAGGTGCAATGAGAAGGATATTCGAACTGCATGGGAGGCCGTGAAGTACGCTAAGAGACCGCGGATACATACGTTTATTGCAACTAGCGAAATTCATATGAAGTATAAGCTGAGGAAGACGAGGGAGGAAGTGATTGAGATTGCTCGAAGTATGGTGCGGTTTGCACGAAGTTTGGGCTGTGATGATGTTGAGTTCAGCCCCGAGGATGCTGGGAGATCTGATAGGGAGTTTCTTTACGAAATTCTGGGTGAAGTTATAAAGGCTGGGGCAACTACTCTCAACATACCAGATACTGTGGGTTATACCATGCCGGTTGAATTTGGGGCGTTGATTGCTGATATAAAAACTAATACGCCTGGCATTGAAAATGTTATCATTTCGACACACTGCCAGAATGATCTTGGACTCGCAACTGCCAACACAGTAGCAGGAGCATGTGCAGGTGCAAGGCAAGTTGAGGTCACAATTAATGGCATTGGTGAGAGAGCTGGAAATGCTTCGCTGGAGGAGGCAAGTTATTTATTTAATTTATTTCCTACTTATTCTTTCATTTATTTTGAAATAATAATCATTCAAGAAGGTTGTGTGGTAATGGCCTTACAATGTCGTGGAGAGCATGTGCTTGGAGGTCTTCATTCTGGAATTAACTCAAGACATATCTTTCTGACTAGCAAGATGGTAGAAGAATATACTGGCTTGAATGTACAACCGCACAAGGCTATTGTTGGAGCTAATGCTTTTGCTCATGAAAGTGGCATCCACCAGGATGGGATGCTTAAGCACAAAGGTACTTATGAAATCATATCCCCTGAAGACATTGGCTACGAACGATCCAATGATGCTGGTATTGTTCTAGGAAAACTTAGTGGACGCCATGCCCTTAAAAGTCGACTTCAAGAGCTTGGTTATGAGCTTGACAATGAGAATCTTGACAACATATTCTGGCGTTTCAAAGCAGTAGCTGAACAAAAGAAGAGAGTAACAGATGCTGATCTTCGAGCATTGGTATCCGATGAAGTTTTTCAACCAAGTGTTGTCTGGAAGCTGCTAGATTTGCAGGTAACCTGTGGAACTCTTGGTCTTTCTACGGCTACCGTTAAACTCCTCAATGCTGATGGTAAAGAGCATACTAATTGTGCTGTTGGAACGGGGCCTGTAGATTCAGCTTACAAGGCTGTAGATGGCATTGTGAAGGAACCAGTTGCACTTCTTGAGTATTCGATGAACGCTGTCACTGAAGGCATTGATGCAATTGCCACGACCCGTGTTGTAATAAGAGGGGAAAACAGTTACACATCTACTAATGCAATTACAGGAGAAGCAGTTCAACGAACATTTAGTGGTATCGGGGCTGGCATGGACATAGTCGTATCAAGTGTGAAGGCCTACATTGGTGCACTAAACAAGATGCTTGGTTTTCAAGGAGTTGACATTAAGGTTTAG

Protein sequence

MAMATAFSSPNHCFGRQWNSQFPTSGHIYRRNLQNPSSFRSPAGRVCCSEAQSISIASDSKPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRTAWEAVKYAKRPRIHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPEDAGRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTHCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEASYLFNLFPTYSFIYFEIIIIQEGCVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRFKAVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLNADGKEHTNCAVGTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAITGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKV

Homology

[Close]

BLAST of MC11g1352 vs. ExPASy Swiss-Prot
Match: Q9C550 (2-isopropylmalate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPMS2 PE=1 SV=1)

HSP 1 Score: 919.5 bits (2375), Expect = 2.1e-266
Identity = 463/581 (79.69%), Postives = 518/581 (89.16%), Query Frame = 0


Query: 53  SISIASDSKPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQL 112
           S+S +S   P++  +RP YIPNRI DP+YVRIFDTTLRDGEQSPGA+LTSKEKLDIARQL
Sbjct: 58  SLSASSPLPPHAPRRRPNYIPNRISDPNYVRIFDTTLRDGEQSPGATLTSKEKLDIARQL 117

Query: 113 AKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRTAWEA 172
           AKLGVDIIEAGFPAASK+DFEAVK IA+ VGN VD++GYVPVICGLSRCN+KDI TAWEA
Sbjct: 118 AKLGVDIIEAGFPAASKDDFEAVKTIAETVGNTVDENGYVPVICGLSRCNKKDIETAWEA 177

Query: 173 VKYAKRPRIHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPEDAGRS 232
           VKYAKRPRIHTFIATS+IH+KYKL+K++EEVIEIAR+MVRFARSLGC+DVEFSPEDAGRS
Sbjct: 178 VKYAKRPRIHTFIATSDIHLKYKLKKSKEEVIEIARNMVRFARSLGCEDVEFSPEDAGRS 237

Query: 233 DREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTHCQND 292
           +RE+LYEILGEVIKAGATTLNIPDTVG T+P EFG LIADIK NTPGI+NVIISTHCQND
Sbjct: 238 EREYLYEILGEVIKAGATTLNIPDTVGITLPSEFGQLIADIKANTPGIQNVIISTHCQND 297

Query: 293 LGLATANTVAGACAGARQVEVTINGIGERAGNASLEEASYLFNLFPTYSFIYFEIIIIQE 352
           LGL+TANT++GA +GARQVEVTINGIGERAGNASLEE                       
Sbjct: 298 LGLSTANTLSGAHSGARQVEVTINGIGERAGNASLEE----------------------- 357

Query: 353 GCVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGI 412
             VVMA++CRG+HVLGGL +GI++RHI +TSKMVEEYTG+  QPHKAIVGANAFAHESGI
Sbjct: 358 --VVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHKAIVGANAFAHESGI 417

Query: 413 HQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNI 472
           HQDGMLKHKGTYEI+SPE+IG ERSNDAGIVLGKLSGRHALK RL ELGY LD+  L N+
Sbjct: 418 HQDGMLKHKGTYEIMSPEEIGLERSNDAGIVLGKLSGRHALKDRLNELGYVLDDGQLSNL 477

Query: 473 FWRFKAVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLNADG 532
           FWRFKAVAEQKKRVTDADL ALVSDEVFQP  VWKLLD+Q+TCGTLGLST+TVKL ++DG
Sbjct: 478 FWRFKAVAEQKKRVTDADLIALVSDEVFQPEAVWKLLDMQITCGTLGLSTSTVKLADSDG 537

Query: 533 KEHTNCAVGTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTS 592
           KEH  C+VGTGPVD+AYKAVD IVKEP  LLEYSMNAVTEGIDAIATTRV+IRG+N+Y+S
Sbjct: 538 KEHVACSVGTGPVDAAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDNNYSS 597

Query: 593 TNAITGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQ 634
           TNA+TGE+V+RTFSG GAGMDIVVSSVKAY+GALNKMLGF+
Sbjct: 598 TNAVTGESVERTFSGTGAGMDIVVSSVKAYVGALNKMLGFK 613

[Close]

BLAST of MC11g1352 vs. ExPASy Swiss-Prot
Match: Q9LPR4 (2-isopropylmalate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPMS1 PE=1 SV=2)

HSP 1 Score: 908.7 bits (2347), Expect = 3.8e-263
Identity = 465/597 (77.89%), Postives = 515/597 (86.26%), Query Frame = 0


Query: 38  SFRSPAGRVCCSEAQSISIASDSKPNSSVQ-RPPYIPNRIPDPSYVRIFDTTLRDGEQSP 97
           S RS   R+ C    SIS  S   P++  + RP YIPNRI DP+YVR+FDTTLRDGEQSP
Sbjct: 48  SLRSQTLRLSC----SISDPSPLPPHTPRRPRPEYIPNRISDPNYVRVFDTTLRDGEQSP 107

Query: 98  GASLTSKEKLDIARQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVIC 157
           GA+LTSKEKLDIARQLAKLGVDIIEAGFPAASK+DFEAVK IA+ VGN VD++GYVPVIC
Sbjct: 108 GATLTSKEKLDIARQLAKLGVDIIEAGFPAASKDDFEAVKTIAETVGNTVDENGYVPVIC 167

Query: 158 GLSRCNEKDIRTAWEAVKYAKRPRIHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARS 217
           GLSRCN+KDI  AW+AVKYAKRPRIHTFIATS+IH++YKL+KT+ EVIEIARSMVRFARS
Sbjct: 168 GLSRCNKKDIERAWDAVKYAKRPRIHTFIATSDIHLEYKLKKTKAEVIEIARSMVRFARS 227

Query: 218 LGCDDVEFSPEDAGRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTN 277
           LGC+DVEFSPEDAGRS+RE+LYEILGEVIKAGATTLNIPDTVG T+P EFG LI D+K N
Sbjct: 228 LGCEDVEFSPEDAGRSEREYLYEILGEVIKAGATTLNIPDTVGITLPSEFGQLITDLKAN 287

Query: 278 TPGIENVIISTHCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEASYLFNL 337
           TPGIENV+ISTHCQNDLGL+TANT++GA AGARQ+EVTINGIGERAGNASLEE       
Sbjct: 288 TPGIENVVISTHCQNDLGLSTANTLSGAHAGARQMEVTINGIGERAGNASLEE------- 347

Query: 338 FPTYSFIYFEIIIIQEGCVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQP 397
                             VVMA++CRG+HVLGGL +GI++RHI +TSKMVEEYTG+  QP
Sbjct: 348 ------------------VVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQP 407

Query: 398 HKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSR 457
           HKAIVGANAFAHESGIHQDGMLKHKGTYEII PE+IG ERSNDAGIVLGKLSGRHALK R
Sbjct: 408 HKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEEIGLERSNDAGIVLGKLSGRHALKDR 467

Query: 458 LQELGYELDNENLDNIFWRFKAVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCG 517
           L ELGY+LD+E L  IFWRFK VAEQKKRVTDAD+ ALVSDEVFQP  VWKLLD+Q+TCG
Sbjct: 468 LTELGYQLDDEQLSTIFWRFKTVAEQKKRVTDADIIALVSDEVFQPEAVWKLLDIQITCG 527

Query: 518 TLGLSTATVKLLNADGKEHTNCAVGTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDA 577
           TLGLSTATVKL +ADGKEH  C++GTGPVDSAYKAVD IVKEP  LLEYSMNAVTEGIDA
Sbjct: 528 TLGLSTATVKLADADGKEHVACSIGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDA 587

Query: 578 IATTRVVIRGENSYTSTNAITGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQ 634
           IATTRV+IRG N Y+STNAITGE VQRTFSG GAGMDIVVSSVKAY+GALNKM+ F+
Sbjct: 588 IATTRVLIRGSNKYSSTNAITGEEVQRTFSGTGAGMDIVVSSVKAYVGALNKMMDFK 615

[Close]

BLAST of MC11g1352 vs. ExPASy Swiss-Prot
Match: O04973 (2-isopropylmalate synthase A OS=Solanum pennellii OX=28526 GN=IPMSA PE=2 SV=1)

HSP 1 Score: 844.7 bits (2181), Expect = 6.7e-244
Identity = 418/569 (73.46%), Postives = 491/569 (86.29%), Query Frame = 0


Query: 65  SVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAKLGVDIIEAGF 124
           S +RP Y+P++I DP YVRIFDTTLRDGEQSPGA++T+KEKLD+ARQLAKLGVDIIEAGF
Sbjct: 31  SNRRPEYVPSKISDPKYVRIFDTTLRDGEQSPGATMTTKEKLDVARQLAKLGVDIIEAGF 90

Query: 125 PAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRTAWEAVKYAKRPRIHTF 184
           PA+S+ DFE+VK+IA+E+GN  D++G+VPVICGLSRCN+ DI  AWEAVKYAK+PR+HTF
Sbjct: 91  PASSEADFESVKLIAEEIGNNTDENGFVPVICGLSRCNKSDIDKAWEAVKYAKKPRVHTF 150

Query: 185 IATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPEDAGRSDREFLYEILGEV 244
           IATSEIHMKYKL+ +RE+V+E ARSMV +ARSLGC+DVEFSPEDAGRSDREFLY+ILGEV
Sbjct: 151 IATSEIHMKYKLKMSREQVVEKARSMVAYARSLGCEDVEFSPEDAGRSDREFLYDILGEV 210

Query: 245 IKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTHCQNDLGLATANTVAGA 304
           IKAGATTLNIPDTVGYT+P EFG LI DIK NTPGIENVIISTHCQNDLGL+TANT+AGA
Sbjct: 211 IKAGATTLNIPDTVGYTVPSEFGQLITDIKANTPGIENVIISTHCQNDLGLSTANTLAGA 270

Query: 305 CAGARQVEVTINGIGERAGNASLEEASYLFNLFPTYSFIYFEIIIIQEGCVVMALQCRGE 364
           CAGARQ+EVTINGIGERAGNASLEE                         VVMAL+CRGE
Sbjct: 271 CAGARQLEVTINGIGERAGNASLEE-------------------------VVMALKCRGE 330

Query: 365 HVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTY 424
            VLGGL++GIN++HI  +SKMVEEY+GL VQPHKAIVGANAFAHESGIHQDGMLKHK TY
Sbjct: 331 QVLGGLYTGINTQHIVPSSKMVEEYSGLQVQPHKAIVGANAFAHESGIHQDGMLKHKDTY 390

Query: 425 EIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRFKAVAEQKK 484
           EIISP+D+G  RSN+AGIVLGKLSGRHALKS++ ELGY++D + L+++FWRFK+VAE+KK
Sbjct: 391 EIISPDDVGLSRSNEAGIVLGKLSGRHALKSKMLELGYDIDGKELEDLFWRFKSVAEKKK 450

Query: 485 RVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLNADGKEHTNCAVGTGP 544
           ++TD DL AL+SDEV QP+V WKL D+Q+ CG+LGLSTATVKL+N DG+EH  C+VGTGP
Sbjct: 451 KITDDDLIALMSDEVLQPNVYWKLGDVQIMCGSLGLSTATVKLINTDGQEHIACSVGTGP 510

Query: 545 VDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAITGEAVQRT 604
           VD+AYKAVD IVK P+ LLEYSMNAVTEGIDAIA+TRV I   + +T  N  TG+ + RT
Sbjct: 511 VDAAYKAVDLIVKVPITLLEYSMNAVTEGIDAIASTRVSICSIDRHTIMNGSTGQTIHRT 570

Query: 605 FSGIGAGMDIVVSSVKAYIGALNKMLGFQ 634
           FSG GA MD+V+SSV+AYIGALNKML ++
Sbjct: 571 FSGTGADMDVVISSVRAYIGALNKMLSYE 574

[Close]

BLAST of MC11g1352 vs. ExPASy Swiss-Prot
Match: O04974 (2-isopropylmalate synthase B OS=Solanum pennellii OX=28526 GN=IPMSB PE=2 SV=1)

HSP 1 Score: 832.0 bits (2148), Expect = 4.5e-240
Identity = 415/570 (72.81%), Postives = 491/570 (86.14%), Query Frame = 0


Query: 65  SVQR-PPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAKLGVDIIEAG 124
           S++R P Y P+ IPDP+YVRIFDTTLRDGEQSPGA++T+KEKLD+ARQ AKLGVDIIEAG
Sbjct: 53  SIRRIPEYTPSHIPDPNYVRIFDTTLRDGEQSPGATMTTKEKLDVARQSAKLGVDIIEAG 112

Query: 125 FPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRTAWEAVKYAKRPRIHT 184
           FPA+S+ D EAVK+IAKEVGN V ++ YVPVICGL+RCN+KDI  AWEAVKYAK+PRIHT
Sbjct: 113 FPASSEADLEAVKLIAKEVGNGVYEEEYVPVICGLARCNKKDIDKAWEAVKYAKKPRIHT 172

Query: 185 FIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPEDAGRSDREFLYEILGE 244
           FIATSE+HM YKL+ +R++V+E ARSMV +ARS+GC+DVEFSPEDAGRSD EFLY ILGE
Sbjct: 173 FIATSEVHMNYKLKMSRDQVVEKARSMVAYARSIGCEDVEFSPEDAGRSDPEFLYHILGE 232

Query: 245 VIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTHCQNDLGLATANTVAG 304
           VIKAGATTLNIPDTVGYT+P EFG LIA IK NTPG+E+VIISTHCQNDLGL+TANT+AG
Sbjct: 233 VIKAGATTLNIPDTVGYTVPEEFGQLIAKIKANTPGVEDVIISTHCQNDLGLSTANTLAG 292

Query: 305 ACAGARQVEVTINGIGERAGNASLEEASYLFNLFPTYSFIYFEIIIIQEGCVVMALQCRG 364
           ACAGARQ+EVTINGIGERAGNASLEE                         VVMAL+CRG
Sbjct: 293 ACAGARQLEVTINGIGERAGNASLEE-------------------------VVMALKCRG 352

Query: 365 EHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGT 424
           E VLGGL++GIN++HI ++SKMVE  +GL+VQPHKAIVGANAF HESGIHQDGMLKHK T
Sbjct: 353 EQVLGGLYTGINTQHILMSSKMVEGISGLHVQPHKAIVGANAFVHESGIHQDGMLKHKDT 412

Query: 425 YEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRFKAVAEQK 484
           YEIISPEDIG  R+N++GIV GKLSG    K ++ ELGYE++ + LD++FWRFK+VAE+K
Sbjct: 413 YEIISPEDIGLNRANESGIVFGKLSGVMLCKPKMLELGYEIEGKELDDLFWRFKSVAEKK 472

Query: 485 KRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLNADGKEHTNCAVGTG 544
           K++TD DL AL+SDEVFQP  VW+L ++QVTCG+LGLSTATVKL++ADG+EH +C+VGTG
Sbjct: 473 KKITDDDLVALMSDEVFQPQFVWQLQNVQVTCGSLGLSTATVKLIDADGREHISCSVGTG 532

Query: 545 PVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAITGEAVQR 604
           PVD+AYKAVD IVK PV LLEYSMNAVT+GIDAIA+TRV+IRGEN +TST+A+TGE V R
Sbjct: 533 PVDAAYKAVDLIVKVPVTLLEYSMNAVTQGIDAIASTRVLIRGENGHTSTHALTGETVHR 592

Query: 605 TFSGIGAGMDIVVSSVKAYIGALNKMLGFQ 634
           TFSG GA MDIV+SSV+AY+GALNKM+ F+
Sbjct: 593 TFSGTGADMDIVISSVRAYVGALNKMMSFR 597

[Close]

BLAST of MC11g1352 vs. ExPASy Swiss-Prot
Match: Q39891 (Probable 2-isopropylmalate synthase OS=Glycine max OX=3847 GN=GMN56 PE=2 SV=1)

HSP 1 Score: 745.0 bits (1922), Expect = 7.2e-214
Identity = 383/576 (66.49%), Postives = 450/576 (78.12%), Query Frame = 0


Query: 55  SIASDSKPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAK 114
           S  S   P  S  RP YIPN IPD SYVRI DTTLRDGEQSPGA++T+KEKLDIARQL K
Sbjct: 6   STPSSQSPKLSHLRPQYIPNHIPDSSYVRILDTTLRDGEQSPGATMTAKEKLDIARQLVK 65

Query: 115 LGVDIIEAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRTAWEAVK 174
           LGVDII+ GFP+AS  DF AVK+IA+EVGN VD DGYVPVI G  RC EKDI TAWEAVK
Sbjct: 66  LGVDIIQPGFPSASNSDFMAVKMIAQEVGNAVDDDGYVPVIAGFCRCVEKDISTAWEAVK 125

Query: 175 YAKRPRIHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPEDAGRSDR 234
           YAKRPR+ T IATS IHM++KLRK++++VI+IAR MV+FARSLGC+D++F  EDA RSDR
Sbjct: 126 YAKRPRLCTSIATSPIHMEHKLRKSKDQVIQIARDMVKFARSLGCNDIQFGAEDATRSDR 185

Query: 235 EFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTHCQNDLG 294
           EFLYEILG VI+AGATT+NI DTVG  MP+E G LI DIK NTPGI NVIISTHC NDLG
Sbjct: 186 EFLYEILGVVIEAGATTVNIADTVGIVMPLELGKLIVDIKDNTPGIANVIISTHCHNDLG 245

Query: 295 LATANTVAGACAGARQVEVTINGIGERAGNASLEEASYLFNLFPTYSFIYFEIIIIQEGC 354
           LATANT+ GA  GARQ+EVTINGIGERAGNASLEE                         
Sbjct: 246 LATANTIEGARTGARQLEVTINGIGERAGNASLEE------------------------- 305

Query: 355 VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 414
           VVMAL  +G+H L GL++ IN+RHI  TSKMVEEY+G+++QPHK +VGANAF H SGIHQ
Sbjct: 306 VVMALASKGDHALNGLYTRINTRHILETSKMVEEYSGMHLQPHKPLVGANAFVHASGIHQ 365

Query: 415 DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW 474
           DGMLKHKGTYE ISPE+IG++R+   GIVLGKLSG  AL+ RL+ELGY+L  + +D++FW
Sbjct: 366 DGMLKHKGTYETISPEEIGHKRTTRIGIVLGKLSGSQALRKRLEELGYDLKEDEVDSVFW 425

Query: 475 RFKAVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLNADGKE 534
           +FKA+AE+KK VTD DL+ALVS + F    +WKL DLQVTCGT+GLSTATVKL+N DG  
Sbjct: 426 QFKAMAEKKKVVTDVDLKALVSYKAFHAESIWKLGDLQVTCGTIGLSTATVKLVNIDGST 485

Query: 535 HTNCAVGTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTSTN 594
           H  C++G G VDS YKA++ IVKEP  LL+YS+N+VTEGI    T RVVI  EN++TST 
Sbjct: 486 HVACSIGIGAVDSTYKAINLIVKEPTKLLDYSLNSVTEGIGVNVTARVVICRENNHTSTY 545

Query: 595 AITGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKML 631
           A T +A   TFSGI A MD+VVS+VKAY+ ALNK+L
Sbjct: 546 AFTEDANYPTFSGIAAEMDVVVSTVKAYLVALNKLL 556

[Close]

BLAST of MC11g1352 vs. NCBI nr
Match: XP_022141882.1 (2-isopropylmalate synthase 2, chloroplastic-like isoform X2 [Momordica charantia])

HSP 1 Score: 1197 bits (3096), Expect = 0.0
Identity = 614/639 (96.09%), Postives = 614/639 (96.09%), Query Frame = 0


Query: 1   MAMATAFSSPNHCFGRQWNSQFPTSGHIYRRNLQNPSSFRSPAGRVCCSEAQSISIASDS 60
           MAMATAFSSPNHCFGRQWNSQFPTSGHIYRRNLQNPSSFRSPAGRVCCSEAQSISIASDS
Sbjct: 1   MAMATAFSSPNHCFGRQWNSQFPTSGHIYRRNLQNPSSFRSPAGRVCCSEAQSISIASDS 60

Query: 61  KPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAKLGVDII 120
           KPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAKLGVDII
Sbjct: 61  KPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAKLGVDII 120

Query: 121 EAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRTAWEAVKYAKRPR 180
           EAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRTAWEAVKYAKRPR
Sbjct: 121 EAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRTAWEAVKYAKRPR 180

Query: 181 IHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPEDAGRSDREFLYEI 240
           IHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPEDAGRSDREFLYEI
Sbjct: 181 IHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPEDAGRSDREFLYEI 240

Query: 241 LGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTHCQNDLGLATANT 300
           LGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTHCQNDLGLATANT
Sbjct: 241 LGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTHCQNDLGLATANT 300

Query: 301 VAGACAGARQVEVTINGIGERAGNASLEEASYLFNLFPTYSFIYFEIIIIQEGCVVMALQ 360
           VAGACAGARQVEVTINGIGERAGNASLEE                         VVMALQ
Sbjct: 301 VAGACAGARQVEVTINGIGERAGNASLEE-------------------------VVMALQ 360

Query: 361 CRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKH 420
           CRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKH
Sbjct: 361 CRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKH 420

Query: 421 KGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRFKAVA 480
           KGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRFKAVA
Sbjct: 421 KGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRFKAVA 480

Query: 481 EQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLNADGKEHTNCAV 540
           EQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLNADGKEHTNCAV
Sbjct: 481 EQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLNADGKEHTNCAV 540

Query: 541 GTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAITGEA 600
           GTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAITGEA
Sbjct: 541 GTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAITGEA 600

Query: 601 VQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKV 639
           VQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKV
Sbjct: 601 VQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKV 614

[Close]

BLAST of MC11g1352 vs. NCBI nr
Match: XP_022141881.1 (2-isopropylmalate synthase 2, chloroplastic-like isoform X1 [Momordica charantia])

HSP 1 Score: 1197 bits (3096), Expect = 0.0
Identity = 614/639 (96.09%), Postives = 614/639 (96.09%), Query Frame = 0


Query: 1   MAMATAFSSPNHCFGRQWNSQFPTSGHIYRRNLQNPSSFRSPAGRVCCSEAQSISIASDS 60
           MAMATAFSSPNHCFGRQWNSQFPTSGHIYRRNLQNPSSFRSPAGRVCCSEAQSISIASDS
Sbjct: 1   MAMATAFSSPNHCFGRQWNSQFPTSGHIYRRNLQNPSSFRSPAGRVCCSEAQSISIASDS 60

Query: 61  KPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAKLGVDII 120
           KPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAKLGVDII
Sbjct: 61  KPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAKLGVDII 120

Query: 121 EAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRTAWEAVKYAKRPR 180
           EAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRTAWEAVKYAKRPR
Sbjct: 121 EAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRTAWEAVKYAKRPR 180

Query: 181 IHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPEDAGRSDREFLYEI 240
           IHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPEDAGRSDREFLYEI
Sbjct: 181 IHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPEDAGRSDREFLYEI 240

Query: 241 LGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTHCQNDLGLATANT 300
           LGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTHCQNDLGLATANT
Sbjct: 241 LGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTHCQNDLGLATANT 300

Query: 301 VAGACAGARQVEVTINGIGERAGNASLEEASYLFNLFPTYSFIYFEIIIIQEGCVVMALQ 360
           VAGACAGARQVEVTINGIGERAGNASLEE                         VVMALQ
Sbjct: 301 VAGACAGARQVEVTINGIGERAGNASLEE-------------------------VVMALQ 360

Query: 361 CRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKH 420
           CRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKH
Sbjct: 361 CRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKH 420

Query: 421 KGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRFKAVA 480
           KGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRFKAVA
Sbjct: 421 KGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRFKAVA 480

Query: 481 EQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLNADGKEHTNCAV 540
           EQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLNADGKEHTNCAV
Sbjct: 481 EQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLNADGKEHTNCAV 540

Query: 541 GTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAITGEA 600
           GTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAITGEA
Sbjct: 541 GTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAITGEA 600

Query: 601 VQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKV 639
           VQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKV
Sbjct: 601 VQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKV 614

[Close]

BLAST of MC11g1352 vs. NCBI nr
Match: XP_038875433.1 (2-isopropylmalate synthase 2, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 1047 bits (2708), Expect = 0.0
Identity = 549/650 (84.46%), Postives = 577/650 (88.77%), Query Frame = 0


Query: 1   MAMATAFSSPN-HCFGRQWNSQFPTSGHIYRRNLQ---NPS---SFRSPAGRV----CCS 60
           MAM  A S PN H   RQ N+QFP    IY  N Q   NPS   SFR+ A  V    CC 
Sbjct: 1   MAMPAALSRPNTHFLSRQSNTQFPARHRIYSTNFQVLKNPSRSLSFRTAADNVSRIYCC- 60

Query: 61  EAQSISIASDSKPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIA 120
             Q+ S+ S+S   SS++RPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLT+KEKLDIA
Sbjct: 61  --QTESVVSNSGLKSSIRRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTAKEKLDIA 120

Query: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRTA 180
           RQLAKLGVDIIEAGFPAASKEDFEAVK+IAKEVGN VD+DGYVPVICGLSRCNE DIRTA
Sbjct: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEVGNAVDEDGYVPVICGLSRCNENDIRTA 180

Query: 181 WEAVKYAKRPRIHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPEDA 240
           WEAVKYAKRPRIHTFIATSEIHM++KLRKTRE+VIEIAR+MVRFARSLGCDDVEFSPEDA
Sbjct: 181 WEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDA 240

Query: 241 GRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTHC 300
           GRSDREFLY+ILGEVIKAGATTLNIPDTVGYTMPVEFG LIADIK+NTPGIENVIISTHC
Sbjct: 241 GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGKLIADIKSNTPGIENVIISTHC 300

Query: 301 QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEASYLFNLFPTYSFIYFEIII 360
           QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEE                    
Sbjct: 301 QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEE-------------------- 360

Query: 361 IQEGCVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHE 420
                VVM+LQCRGEHVLGGLH+GINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHE
Sbjct: 361 -----VVMSLQCRGEHVLGGLHTGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHE 420

Query: 421 SGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENL 480
           SGIHQDGMLKHKGTYEIISPEDIGYERSN+AGIVLGKLSGRHALKSRL ELGYELD+ENL
Sbjct: 421 SGIHQDGMLKHKGTYEIISPEDIGYERSNEAGIVLGKLSGRHALKSRLLELGYELDSENL 480

Query: 481 DNIFWRFKAVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLN 540
           DN+FWRFKAVAEQKKRVTDADLRALVSDEVFQP+V+WKLLDLQVTCGTLGLSTATVKLL+
Sbjct: 481 DNVFWRFKAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDLQVTCGTLGLSTATVKLLD 540

Query: 541 ADGKEHTNCAVGTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENS 600
           ADGKEH  CAVGTGPVDSAYKAVD IVKEP  LLEYSMNAVTEGIDAIATTRV+IRG+ S
Sbjct: 541 ADGKEHIACAVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKS 600

Query: 601 YTSTNAITGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKV 639
           YT+TNA+TGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGF GVDIKV
Sbjct: 601 YTATNALTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFHGVDIKV 622

[Close]

BLAST of MC11g1352 vs. NCBI nr
Match: XP_011651428.1 (2-isopropylmalate synthase 2, chloroplastic [Cucumis sativus] >KGN57885.1 hypothetical protein Csa_010376 [Cucumis sativus])

HSP 1 Score: 1037 bits (2682), Expect = 0.0
Identity = 540/654 (82.57%), Postives = 574/654 (87.77%), Query Frame = 0


Query: 1   MAMATAFSSPNHCFGRQWNSQFPTSGHIYRRNLQ---NPS---SFRSPAGRV-------- 60
           MA+  A S PNH   RQ +S FP    IY RNLQ   NPS   SF++ A           
Sbjct: 1   MALPAALSRPNHSLARQSDSLFPAPHRIYSRNLQVLKNPSRSLSFKTAAASAPHNVSPVY 60

Query: 61  -CCSEAQSISIASDSKPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEK 120
            C +E    SI S+S P +S++RPPYIPNRIPDPSYVR+FDTTLRDGEQSPGASLT KEK
Sbjct: 61  SCLTE----SIVSNSVPKASIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEK 120

Query: 121 LDIARQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKD 180
           LDIARQLAKLGVDIIEAGFPAASKEDFEAVK+IAKE+GN VD+DGYVPVICGLSRCNEKD
Sbjct: 121 LDIARQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCNEKD 180

Query: 181 IRTAWEAVKYAKRPRIHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFS 240
           IRTAWEAVKYAKRPRIHTFIATSEIHM++KLRKT+EEVIEIAR+MVRFARSLGCDDVEFS
Sbjct: 181 IRTAWEAVKYAKRPRIHTFIATSEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFS 240

Query: 241 PEDAGRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVII 300
           PEDAGRSDREFLY+ILGEVIKAGATTLNIPDTVGYTMP EFG LIADIK+NTPGIENVII
Sbjct: 241 PEDAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVII 300

Query: 301 STHCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEASYLFNLFPTYSFIYF 360
           S+HCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEE                
Sbjct: 301 SSHCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEE---------------- 360

Query: 361 EIIIIQEGCVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANA 420
                    VVMALQCRGEHVLGGLH+GINSRHIFLTSKMVEE+TGLNVQPHKAIVGANA
Sbjct: 361 ---------VVMALQCRGEHVLGGLHTGINSRHIFLTSKMVEEFTGLNVQPHKAIVGANA 420

Query: 421 FAHESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELD 480
           FAH SGIHQDGMLKHKGTYEI++PEDIGYERSNDAGIVLGKLSGRHALKS L ELGYELD
Sbjct: 421 FAHASGIHQDGMLKHKGTYEIMAPEDIGYERSNDAGIVLGKLSGRHALKSLLLELGYELD 480

Query: 481 NENLDNIFWRFKAVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATV 540
            ENLDN+FWRFKAVAEQKKRVTDADLRALVSDEVFQP+V+WKLLD+QVTCGTLGLSTATV
Sbjct: 481 GENLDNVFWRFKAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDMQVTCGTLGLSTATV 540

Query: 541 KLLNADGKEHTNCAVGTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIR 600
           KLL+ADGKEH  C+VGTGPVDSAYKAVD IVKEP  LLEYSMNAVTEGIDAIATTRV+IR
Sbjct: 541 KLLDADGKEHIACSVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIR 600

Query: 601 GENSYTSTNAITGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKV 639
           G+ SYTSTNA+TGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQG+DIKV
Sbjct: 601 GDKSYTSTNALTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGIDIKV 625

[Close]

BLAST of MC11g1352 vs. NCBI nr
Match: TYK01483.1 (2-isopropylmalate synthase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1035 bits (2677), Expect = 0.0
Identity = 539/650 (82.92%), Postives = 573/650 (88.15%), Query Frame = 0


Query: 1   MAMATAFSSPNHCFGRQWNSQFPTSGHIYRRNLQ---NPS---SFRSPAG------RVCC 60
           MA+  A S PNH   RQ +S FP    IY RN Q   NPS   SF++ A       R+ C
Sbjct: 1   MALPAALSRPNHFLARQSDSLFPARHPIYTRNFQVLKNPSRSLSFKTAAAAPDNVSRIYC 60

Query: 61  SEAQSISIASDSKPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDI 120
              +S+   S+S P SS +RPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLT+KEKLDI
Sbjct: 61  CLTESV--VSNSGPKSSTRRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTTKEKLDI 120

Query: 121 ARQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRT 180
           ARQLAKLGVDIIEAGFPAASKEDFEAVK+IAKE+G+ VD+DGYVPVICGLSRCNEKDIRT
Sbjct: 121 ARQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGSAVDEDGYVPVICGLSRCNEKDIRT 180

Query: 181 AWEAVKYAKRPRIHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPED 240
           AWEAVKYAKRPRIHTFIATSEIHM++KLRKTRE+VIEIAR+MVRFARSLGCDDVEFSPED
Sbjct: 181 AWEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPED 240

Query: 241 AGRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTH 300
           AGRSDREFLY+ILGEVIKAGATTLNIPDTVGYTMP EFG LIADIK+NTPGIENVIISTH
Sbjct: 241 AGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISTH 300

Query: 301 CQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEASYLFNLFPTYSFIYFEII 360
           CQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEE                   
Sbjct: 301 CQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEE------------------- 360

Query: 361 IIQEGCVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAH 420
                 VVMALQCRGEHVLGGLH+GIN RHIFLTSKMVEE+TGLNVQPHKAIVGANAFAH
Sbjct: 361 ------VVMALQCRGEHVLGGLHTGINPRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAH 420

Query: 421 ESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNEN 480
            SGIHQDGMLKHKGTYEI++PEDIGYERSNDAGIVLGKLSGRHALKSRL ELGYELD+EN
Sbjct: 421 ASGIHQDGMLKHKGTYEIMAPEDIGYERSNDAGIVLGKLSGRHALKSRLLELGYELDSEN 480

Query: 481 LDNIFWRFKAVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLL 540
           L N+FWRFKAVAEQKKRVTDADLRALVSDEVFQP+V+WKLLDLQVTCGTLGLSTATVKLL
Sbjct: 481 LHNVFWRFKAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDLQVTCGTLGLSTATVKLL 540

Query: 541 NADGKEHTNCAVGTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGEN 600
           +ADGKEH  C+VGTGPVDSAYKAVD IVKEP  LLEYSMNAVTEGIDAIATTRV+IRG+ 
Sbjct: 541 DADGKEHIACSVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDK 600

Query: 601 SYTSTNAITGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIK 638
           SYTSTNA+TGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQG+DIK
Sbjct: 601 SYTSTNALTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGIDIK 623

[Close]

BLAST of MC11g1352 vs. ExPASy TrEMBL
Match: A0A6J1CL37 (2-isopropylmalate synthase OS=Momordica charantia OX=3673 GN=LOC111012138 PE=3 SV=1)

HSP 1 Score: 1197 bits (3096), Expect = 0.0
Identity = 614/639 (96.09%), Postives = 614/639 (96.09%), Query Frame = 0


Query: 1   MAMATAFSSPNHCFGRQWNSQFPTSGHIYRRNLQNPSSFRSPAGRVCCSEAQSISIASDS 60
           MAMATAFSSPNHCFGRQWNSQFPTSGHIYRRNLQNPSSFRSPAGRVCCSEAQSISIASDS
Sbjct: 1   MAMATAFSSPNHCFGRQWNSQFPTSGHIYRRNLQNPSSFRSPAGRVCCSEAQSISIASDS 60

Query: 61  KPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAKLGVDII 120
           KPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAKLGVDII
Sbjct: 61  KPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAKLGVDII 120

Query: 121 EAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRTAWEAVKYAKRPR 180
           EAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRTAWEAVKYAKRPR
Sbjct: 121 EAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRTAWEAVKYAKRPR 180

Query: 181 IHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPEDAGRSDREFLYEI 240
           IHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPEDAGRSDREFLYEI
Sbjct: 181 IHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPEDAGRSDREFLYEI 240

Query: 241 LGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTHCQNDLGLATANT 300
           LGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTHCQNDLGLATANT
Sbjct: 241 LGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTHCQNDLGLATANT 300

Query: 301 VAGACAGARQVEVTINGIGERAGNASLEEASYLFNLFPTYSFIYFEIIIIQEGCVVMALQ 360
           VAGACAGARQVEVTINGIGERAGNASLEE                         VVMALQ
Sbjct: 301 VAGACAGARQVEVTINGIGERAGNASLEE-------------------------VVMALQ 360

Query: 361 CRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKH 420
           CRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKH
Sbjct: 361 CRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKH 420

Query: 421 KGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRFKAVA 480
           KGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRFKAVA
Sbjct: 421 KGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRFKAVA 480

Query: 481 EQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLNADGKEHTNCAV 540
           EQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLNADGKEHTNCAV
Sbjct: 481 EQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLNADGKEHTNCAV 540

Query: 541 GTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAITGEA 600
           GTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAITGEA
Sbjct: 541 GTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAITGEA 600

Query: 601 VQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKV 639
           VQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKV
Sbjct: 601 VQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKV 614

[Close]

BLAST of MC11g1352 vs. ExPASy TrEMBL
Match: A0A6J1CKJ3 (2-isopropylmalate synthase OS=Momordica charantia OX=3673 GN=LOC111012138 PE=3 SV=1)

HSP 1 Score: 1197 bits (3096), Expect = 0.0
Identity = 614/639 (96.09%), Postives = 614/639 (96.09%), Query Frame = 0


Query: 1   MAMATAFSSPNHCFGRQWNSQFPTSGHIYRRNLQNPSSFRSPAGRVCCSEAQSISIASDS 60
           MAMATAFSSPNHCFGRQWNSQFPTSGHIYRRNLQNPSSFRSPAGRVCCSEAQSISIASDS
Sbjct: 1   MAMATAFSSPNHCFGRQWNSQFPTSGHIYRRNLQNPSSFRSPAGRVCCSEAQSISIASDS 60

Query: 61  KPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAKLGVDII 120
           KPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAKLGVDII
Sbjct: 61  KPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAKLGVDII 120

Query: 121 EAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRTAWEAVKYAKRPR 180
           EAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRTAWEAVKYAKRPR
Sbjct: 121 EAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRTAWEAVKYAKRPR 180

Query: 181 IHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPEDAGRSDREFLYEI 240
           IHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPEDAGRSDREFLYEI
Sbjct: 181 IHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPEDAGRSDREFLYEI 240

Query: 241 LGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTHCQNDLGLATANT 300
           LGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTHCQNDLGLATANT
Sbjct: 241 LGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTHCQNDLGLATANT 300

Query: 301 VAGACAGARQVEVTINGIGERAGNASLEEASYLFNLFPTYSFIYFEIIIIQEGCVVMALQ 360
           VAGACAGARQVEVTINGIGERAGNASLEE                         VVMALQ
Sbjct: 301 VAGACAGARQVEVTINGIGERAGNASLEE-------------------------VVMALQ 360

Query: 361 CRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKH 420
           CRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKH
Sbjct: 361 CRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKH 420

Query: 421 KGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRFKAVA 480
           KGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRFKAVA
Sbjct: 421 KGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRFKAVA 480

Query: 481 EQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLNADGKEHTNCAV 540
           EQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLNADGKEHTNCAV
Sbjct: 481 EQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLNADGKEHTNCAV 540

Query: 541 GTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAITGEA 600
           GTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAITGEA
Sbjct: 541 GTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAITGEA 600

Query: 601 VQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKV 639
           VQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKV
Sbjct: 601 VQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKV 614

[Close]

BLAST of MC11g1352 vs. ExPASy TrEMBL
Match: A0A0A0L7C8 (2-isopropylmalate synthase OS=Cucumis sativus OX=3659 GN=Csa_3G363180 PE=3 SV=1)

HSP 1 Score: 1037 bits (2682), Expect = 0.0
Identity = 540/654 (82.57%), Postives = 574/654 (87.77%), Query Frame = 0


Query: 1   MAMATAFSSPNHCFGRQWNSQFPTSGHIYRRNLQ---NPS---SFRSPAGRV-------- 60
           MA+  A S PNH   RQ +S FP    IY RNLQ   NPS   SF++ A           
Sbjct: 1   MALPAALSRPNHSLARQSDSLFPAPHRIYSRNLQVLKNPSRSLSFKTAAASAPHNVSPVY 60

Query: 61  -CCSEAQSISIASDSKPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEK 120
            C +E    SI S+S P +S++RPPYIPNRIPDPSYVR+FDTTLRDGEQSPGASLT KEK
Sbjct: 61  SCLTE----SIVSNSVPKASIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEK 120

Query: 121 LDIARQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKD 180
           LDIARQLAKLGVDIIEAGFPAASKEDFEAVK+IAKE+GN VD+DGYVPVICGLSRCNEKD
Sbjct: 121 LDIARQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCNEKD 180

Query: 181 IRTAWEAVKYAKRPRIHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFS 240
           IRTAWEAVKYAKRPRIHTFIATSEIHM++KLRKT+EEVIEIAR+MVRFARSLGCDDVEFS
Sbjct: 181 IRTAWEAVKYAKRPRIHTFIATSEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFS 240

Query: 241 PEDAGRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVII 300
           PEDAGRSDREFLY+ILGEVIKAGATTLNIPDTVGYTMP EFG LIADIK+NTPGIENVII
Sbjct: 241 PEDAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVII 300

Query: 301 STHCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEASYLFNLFPTYSFIYF 360
           S+HCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEE                
Sbjct: 301 SSHCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEE---------------- 360

Query: 361 EIIIIQEGCVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANA 420
                    VVMALQCRGEHVLGGLH+GINSRHIFLTSKMVEE+TGLNVQPHKAIVGANA
Sbjct: 361 ---------VVMALQCRGEHVLGGLHTGINSRHIFLTSKMVEEFTGLNVQPHKAIVGANA 420

Query: 421 FAHESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELD 480
           FAH SGIHQDGMLKHKGTYEI++PEDIGYERSNDAGIVLGKLSGRHALKS L ELGYELD
Sbjct: 421 FAHASGIHQDGMLKHKGTYEIMAPEDIGYERSNDAGIVLGKLSGRHALKSLLLELGYELD 480

Query: 481 NENLDNIFWRFKAVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATV 540
            ENLDN+FWRFKAVAEQKKRVTDADLRALVSDEVFQP+V+WKLLD+QVTCGTLGLSTATV
Sbjct: 481 GENLDNVFWRFKAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDMQVTCGTLGLSTATV 540

Query: 541 KLLNADGKEHTNCAVGTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIR 600
           KLL+ADGKEH  C+VGTGPVDSAYKAVD IVKEP  LLEYSMNAVTEGIDAIATTRV+IR
Sbjct: 541 KLLDADGKEHIACSVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIR 600

Query: 601 GENSYTSTNAITGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIKV 639
           G+ SYTSTNA+TGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQG+DIKV
Sbjct: 601 GDKSYTSTNALTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGIDIKV 625

[Close]

BLAST of MC11g1352 vs. ExPASy TrEMBL
Match: A0A5D3BP53 (2-isopropylmalate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G00350 PE=3 SV=1)

HSP 1 Score: 1035 bits (2677), Expect = 0.0
Identity = 539/650 (82.92%), Postives = 573/650 (88.15%), Query Frame = 0


Query: 1   MAMATAFSSPNHCFGRQWNSQFPTSGHIYRRNLQ---NPS---SFRSPAG------RVCC 60
           MA+  A S PNH   RQ +S FP    IY RN Q   NPS   SF++ A       R+ C
Sbjct: 1   MALPAALSRPNHFLARQSDSLFPARHPIYTRNFQVLKNPSRSLSFKTAAAAPDNVSRIYC 60

Query: 61  SEAQSISIASDSKPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDI 120
              +S+   S+S P SS +RPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLT+KEKLDI
Sbjct: 61  CLTESV--VSNSGPKSSTRRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTTKEKLDI 120

Query: 121 ARQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRT 180
           ARQLAKLGVDIIEAGFPAASKEDFEAVK+IAKE+G+ VD+DGYVPVICGLSRCNEKDIRT
Sbjct: 121 ARQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGSAVDEDGYVPVICGLSRCNEKDIRT 180

Query: 181 AWEAVKYAKRPRIHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPED 240
           AWEAVKYAKRPRIHTFIATSEIHM++KLRKTRE+VIEIAR+MVRFARSLGCDDVEFSPED
Sbjct: 181 AWEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPED 240

Query: 241 AGRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTH 300
           AGRSDREFLY+ILGEVIKAGATTLNIPDTVGYTMP EFG LIADIK+NTPGIENVIISTH
Sbjct: 241 AGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISTH 300

Query: 301 CQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEASYLFNLFPTYSFIYFEII 360
           CQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEE                   
Sbjct: 301 CQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEE------------------- 360

Query: 361 IIQEGCVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAH 420
                 VVMALQCRGEHVLGGLH+GIN RHIFLTSKMVEE+TGLNVQPHKAIVGANAFAH
Sbjct: 361 ------VVMALQCRGEHVLGGLHTGINPRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAH 420

Query: 421 ESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNEN 480
            SGIHQDGMLKHKGTYEI++PEDIGYERSNDAGIVLGKLSGRHALKSRL ELGYELD+EN
Sbjct: 421 ASGIHQDGMLKHKGTYEIMAPEDIGYERSNDAGIVLGKLSGRHALKSRLLELGYELDSEN 480

Query: 481 LDNIFWRFKAVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLL 540
           L N+FWRFKAVAEQKKRVTDADLRALVSDEVFQP+V+WKLLDLQVTCGTLGLSTATVKLL
Sbjct: 481 LHNVFWRFKAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDLQVTCGTLGLSTATVKLL 540

Query: 541 NADGKEHTNCAVGTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGEN 600
           +ADGKEH  C+VGTGPVDSAYKAVD IVKEP  LLEYSMNAVTEGIDAIATTRV+IRG+ 
Sbjct: 541 DADGKEHIACSVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDK 600

Query: 601 SYTSTNAITGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIK 638
           SYTSTNA+TGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQG+DIK
Sbjct: 601 SYTSTNALTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGIDIK 623

[Close]

BLAST of MC11g1352 vs. ExPASy TrEMBL
Match: A0A1S3C4S6 (2-isopropylmalate synthase OS=Cucumis melo OX=3656 GN=LOC103496846 PE=3 SV=1)

HSP 1 Score: 1034 bits (2674), Expect = 0.0
Identity = 539/653 (82.54%), Postives = 573/653 (87.75%), Query Frame = 0


Query: 1   MAMATAFSSPNHCFGRQWNSQFPTSGHIYRRNLQ---NPS---SFRSPAG---------R 60
           MA+  A S PNH   RQ +S FP    IY RN Q   NPS   SF++ A          R
Sbjct: 1   MALPAALSRPNHFLARQSDSLFPARHPIYTRNFQVLKNPSRSLSFKTAAAAAAAPDNVSR 60

Query: 61  VCCSEAQSISIASDSKPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEK 120
           + C   +S+   S+S P SS +RPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLT+KEK
Sbjct: 61  IYCCLTESV--VSNSGPKSSTRRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTTKEK 120

Query: 121 LDIARQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKD 180
           LDIARQLAKLGVDIIEAGFPAASKEDFEAVK+IAKE+G+ VD+DGYVPVICGLSRCNEKD
Sbjct: 121 LDIARQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGSAVDEDGYVPVICGLSRCNEKD 180

Query: 181 IRTAWEAVKYAKRPRIHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFS 240
           IRTAWEAVKYAKRPRIHTFIATSEIHM++KLRKTRE+VIEIAR+MVRFARSLGCDDVEFS
Sbjct: 181 IRTAWEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFS 240

Query: 241 PEDAGRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVII 300
           PEDAGRSDREFLY+ILGEVIKAGATTLNIPDTVGYTMP EFG LIADIK+NTPGIENVII
Sbjct: 241 PEDAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVII 300

Query: 301 STHCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEASYLFNLFPTYSFIYF 360
           STHCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEE                
Sbjct: 301 STHCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEE---------------- 360

Query: 361 EIIIIQEGCVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANA 420
                    VVMALQCRGEHVLGGLH+GIN RHIFLTSKMVEE+TGLNVQPHKAIVGANA
Sbjct: 361 ---------VVMALQCRGEHVLGGLHTGINPRHIFLTSKMVEEFTGLNVQPHKAIVGANA 420

Query: 421 FAHESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELD 480
           FAH SGIHQDGMLKHKGTYEI++PEDIGYERSNDAGIVLGKLSGRHALKSRL ELGYELD
Sbjct: 421 FAHASGIHQDGMLKHKGTYEIMAPEDIGYERSNDAGIVLGKLSGRHALKSRLLELGYELD 480

Query: 481 NENLDNIFWRFKAVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATV 540
           +ENL N+FWRFKAVAEQKKRVTDADLRALVSDEVFQP+V+WKLLDLQVTCGTLGLSTATV
Sbjct: 481 SENLHNVFWRFKAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDLQVTCGTLGLSTATV 540

Query: 541 KLLNADGKEHTNCAVGTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIR 600
           KLL+ADGKEH  C+VGTGPVDSAYKAVD IVKEP  LLEYSMNAVTEGIDAIATTRV+IR
Sbjct: 541 KLLDADGKEHIACSVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIR 600

Query: 601 GENSYTSTNAITGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIK 638
           G+ SYTSTNA+TGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQG+DIK
Sbjct: 601 GDKSYTSTNALTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGIDIK 626

[Close]

BLAST of MC11g1352 vs. TAIR 10
Match: AT1G74040.1 (2-isopropylmalate synthase 1 )

HSP 1 Score: 919.5 bits (2375), Expect = 1.5e-267
Identity = 463/581 (79.69%), Postives = 518/581 (89.16%), Query Frame = 0


Query: 53  SISIASDSKPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQL 112
           S+S +S   P++  +RP YIPNRI DP+YVRIFDTTLRDGEQSPGA+LTSKEKLDIARQL
Sbjct: 58  SLSASSPLPPHAPRRRPNYIPNRISDPNYVRIFDTTLRDGEQSPGATLTSKEKLDIARQL 117

Query: 113 AKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRTAWEA 172
           AKLGVDIIEAGFPAASK+DFEAVK IA+ VGN VD++GYVPVICGLSRCN+KDI TAWEA
Sbjct: 118 AKLGVDIIEAGFPAASKDDFEAVKTIAETVGNTVDENGYVPVICGLSRCNKKDIETAWEA 177

Query: 173 VKYAKRPRIHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPEDAGRS 232
           VKYAKRPRIHTFIATS+IH+KYKL+K++EEVIEIAR+MVRFARSLGC+DVEFSPEDAGRS
Sbjct: 178 VKYAKRPRIHTFIATSDIHLKYKLKKSKEEVIEIARNMVRFARSLGCEDVEFSPEDAGRS 237

Query: 233 DREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTHCQND 292
           +RE+LYEILGEVIKAGATTLNIPDTVG T+P EFG LIADIK NTPGI+NVIISTHCQND
Sbjct: 238 EREYLYEILGEVIKAGATTLNIPDTVGITLPSEFGQLIADIKANTPGIQNVIISTHCQND 297

Query: 293 LGLATANTVAGACAGARQVEVTINGIGERAGNASLEEASYLFNLFPTYSFIYFEIIIIQE 352
           LGL+TANT++GA +GARQVEVTINGIGERAGNASLEE                       
Sbjct: 298 LGLSTANTLSGAHSGARQVEVTINGIGERAGNASLEE----------------------- 357

Query: 353 GCVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGI 412
             VVMA++CRG+HVLGGL +GI++RHI +TSKMVEEYTG+  QPHKAIVGANAFAHESGI
Sbjct: 358 --VVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHKAIVGANAFAHESGI 417

Query: 413 HQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNI 472
           HQDGMLKHKGTYEI+SPE+IG ERSNDAGIVLGKLSGRHALK RL ELGY LD+  L N+
Sbjct: 418 HQDGMLKHKGTYEIMSPEEIGLERSNDAGIVLGKLSGRHALKDRLNELGYVLDDGQLSNL 477

Query: 473 FWRFKAVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLNADG 532
           FWRFKAVAEQKKRVTDADL ALVSDEVFQP  VWKLLD+Q+TCGTLGLST+TVKL ++DG
Sbjct: 478 FWRFKAVAEQKKRVTDADLIALVSDEVFQPEAVWKLLDMQITCGTLGLSTSTVKLADSDG 537

Query: 533 KEHTNCAVGTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTS 592
           KEH  C+VGTGPVD+AYKAVD IVKEP  LLEYSMNAVTEGIDAIATTRV+IRG+N+Y+S
Sbjct: 538 KEHVACSVGTGPVDAAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDNNYSS 597

Query: 593 TNAITGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQ 634
           TNA+TGE+V+RTFSG GAGMDIVVSSVKAY+GALNKMLGF+
Sbjct: 598 TNAVTGESVERTFSGTGAGMDIVVSSVKAYVGALNKMLGFK 613

[Close]

BLAST of MC11g1352 vs. TAIR 10
Match: AT1G18500.1 (methylthioalkylmalate synthase-like 4 )

HSP 1 Score: 908.7 bits (2347), Expect = 2.7e-264
Identity = 465/597 (77.89%), Postives = 515/597 (86.26%), Query Frame = 0


Query: 38  SFRSPAGRVCCSEAQSISIASDSKPNSSVQ-RPPYIPNRIPDPSYVRIFDTTLRDGEQSP 97
           S RS   R+ C    SIS  S   P++  + RP YIPNRI DP+YVR+FDTTLRDGEQSP
Sbjct: 48  SLRSQTLRLSC----SISDPSPLPPHTPRRPRPEYIPNRISDPNYVRVFDTTLRDGEQSP 107

Query: 98  GASLTSKEKLDIARQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVIC 157
           GA+LTSKEKLDIARQLAKLGVDIIEAGFPAASK+DFEAVK IA+ VGN VD++GYVPVIC
Sbjct: 108 GATLTSKEKLDIARQLAKLGVDIIEAGFPAASKDDFEAVKTIAETVGNTVDENGYVPVIC 167

Query: 158 GLSRCNEKDIRTAWEAVKYAKRPRIHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARS 217
           GLSRCN+KDI  AW+AVKYAKRPRIHTFIATS+IH++YKL+KT+ EVIEIARSMVRFARS
Sbjct: 168 GLSRCNKKDIERAWDAVKYAKRPRIHTFIATSDIHLEYKLKKTKAEVIEIARSMVRFARS 227

Query: 218 LGCDDVEFSPEDAGRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTN 277
           LGC+DVEFSPEDAGRS+RE+LYEILGEVIKAGATTLNIPDTVG T+P EFG LI D+K N
Sbjct: 228 LGCEDVEFSPEDAGRSEREYLYEILGEVIKAGATTLNIPDTVGITLPSEFGQLITDLKAN 287

Query: 278 TPGIENVIISTHCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEASYLFNL 337
           TPGIENV+ISTHCQNDLGL+TANT++GA AGARQ+EVTINGIGERAGNASLEE       
Sbjct: 288 TPGIENVVISTHCQNDLGLSTANTLSGAHAGARQMEVTINGIGERAGNASLEE------- 347

Query: 338 FPTYSFIYFEIIIIQEGCVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQP 397
                             VVMA++CRG+HVLGGL +GI++RHI +TSKMVEEYTG+  QP
Sbjct: 348 ------------------VVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQP 407

Query: 398 HKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSR 457
           HKAIVGANAFAHESGIHQDGMLKHKGTYEII PE+IG ERSNDAGIVLGKLSGRHALK R
Sbjct: 408 HKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEEIGLERSNDAGIVLGKLSGRHALKDR 467

Query: 458 LQELGYELDNENLDNIFWRFKAVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCG 517
           L ELGY+LD+E L  IFWRFK VAEQKKRVTDAD+ ALVSDEVFQP  VWKLLD+Q+TCG
Sbjct: 468 LTELGYQLDDEQLSTIFWRFKTVAEQKKRVTDADIIALVSDEVFQPEAVWKLLDIQITCG 527

Query: 518 TLGLSTATVKLLNADGKEHTNCAVGTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDA 577
           TLGLSTATVKL +ADGKEH  C++GTGPVDSAYKAVD IVKEP  LLEYSMNAVTEGIDA
Sbjct: 528 TLGLSTATVKLADADGKEHVACSIGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDA 587

Query: 578 IATTRVVIRGENSYTSTNAITGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQ 634
           IATTRV+IRG N Y+STNAITGE VQRTFSG GAGMDIVVSSVKAY+GALNKM+ F+
Sbjct: 588 IATTRVLIRGSNKYSSTNAITGEEVQRTFSGTGAGMDIVVSSVKAYVGALNKMMDFK 615

[Close]

BLAST of MC11g1352 vs. TAIR 10
Match: AT5G23010.1 (methylthioalkylmalate synthase 1 )

HSP 1 Score: 543.9 bits (1400), Expect = 1.7e-154
Identity = 282/484 (58.26%), Postives = 359/484 (74.17%), Query Frame = 0


Query: 22  FPTSGHIYR--RNLQNPSSFRSPAGRVCCSEAQSISIASDSKPNSSVQR-PPYIPNRIPD 81
           F +S H  R   + +NP+ F S     CCS     +  S +     V+R P YIPN++PD
Sbjct: 27  FQSSLHSLRLTHSYKNPALFIS-----CCSSVSKNAATSSTDLKPVVERWPEYIPNKLPD 86

Query: 82  PSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAKLGVDIIEAGFPAASKEDFEAVKII 141
            +YVR+FDTTLRDGEQSPG SLT  +KL+IARQLAKL VDI+E GFP +S+E+ E +K I
Sbjct: 87  GNYVRVFDTTLRDGEQSPGGSLTPPQKLEIARQLAKLRVDIMEVGFPGSSEEELETIKTI 146

Query: 142 AKEVGNVVDQD-GYVPVICGLSRCNEKDIRTAWEAVKYAKRPRIHTFIATSEIHMKYKLR 201
           AK VGN VD++ GYVPVIC ++RC  +DI   WEA+KYAKRPRI  F +TS+IHMKYKL+
Sbjct: 147 AKTVGNEVDEETGYVPVICAIARCKHRDIEATWEALKYAKRPRILVFTSTSDIHMKYKLK 206

Query: 202 KTREEVIEIARSMVRFARSLGCDDVEFSPEDAGRSDREFLYEILGEVIKAGATTLNIPDT 261
           KT+EEVIE+A S +RFA+SLG +D++F  ED GRSD++FL +ILGE IKAG T + I DT
Sbjct: 207 KTQEEVIEMAVSSIRFAKSLGFNDIQFGCEDGGRSDKDFLCKILGEAIKAGVTVVTIGDT 266

Query: 262 VGYTMPVEFGALIADIKTNTPGIENVIISTHCQNDLGLATANTVAGACAGARQVEVTING 321
           VG  MP E+G L+  +K NTPGI++V+++ HC NDLGLATAN++AG  AGARQVEVTING
Sbjct: 267 VGINMPHEYGELVTYLKANTPGIDDVVVAVHCHNDLGLATANSIAGIRAGARQVEVTING 326

Query: 322 IGERAGNASLEEASYLFNLFPTYSFIYFEIIIIQEGCVVMALQCRGEHVLGGLHSGINSR 381
           IGER+GNASLEE                         VVMAL+CRG +V+ G+++ I++R
Sbjct: 327 IGERSGNASLEE-------------------------VVMALKCRGAYVINGVYTKIDTR 386

Query: 382 HIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERS 441
            I  TSKMV+EYTGL VQ HK IVGAN F HESGIHQDG+LK++ TYEI+SPEDIG  +S
Sbjct: 387 QIMATSKMVQEYTGLYVQAHKPIVGANCFVHESGIHQDGILKNRSTYEILSPEDIGIVKS 446

Query: 442 NDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRFKAVAEQKKRVTDADLRALV-- 500
            ++G+VLGKLSGRHA+K RL+ELGYELD+E L+ +F  F+ + + KKR+TDADL+ALV  
Sbjct: 447 QNSGLVLGKLSGRHAVKDRLKELGYELDDEKLNAVFSLFRDLTKNKKRITDADLKALVTS 480

[Close]

BLAST of MC11g1352 vs. TAIR 10
Match: AT5G23020.1 (2-isopropylmalate synthase 2 )

HSP 1 Score: 534.6 bits (1376), Expect = 1.1e-151
Identity = 266/452 (58.85%), Postives = 350/452 (77.43%), Query Frame = 0


Query: 47  CCS-EAQSISI-ASDSKPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKE 106
           CCS E++ ++  A+D KP    +RP YIPN++P  +YVR+ DTTLRDGEQSPGA+LT  +
Sbjct: 49  CCSAESKKVATSATDLKPIME-RRPEYIPNKLPHKNYVRVLDTTLRDGEQSPGAALTPPQ 108

Query: 107 KLDIARQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNVVDQD-GYVPVICGLSRCNE 166
           KL+IARQLAKL VDI+E GFP +S+E+FEA+K IAK VGN VD++ GYVPVICG++RC +
Sbjct: 109 KLEIARQLAKLRVDIMEVGFPVSSEEEFEAIKTIAKTVGNEVDEETGYVPVICGIARCKK 168

Query: 167 KDIRTAWEAVKYAKRPRIHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVE 226
           +DI   WEA+KYAKRPR+  F +TSEIHMKYKL+KT+EEVIE+A + V++A+SLG  D++
Sbjct: 169 RDIEATWEALKYAKRPRVMLFTSTSEIHMKYKLKKTKEEVIEMAVNSVKYAKSLGFKDIQ 228

Query: 227 FSPEDAGRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENV 286
           F  ED GR++++F+ +ILGE IKAGATT+   DTVG  MP EFG L+A +  NTPG +++
Sbjct: 229 FGCEDGGRTEKDFICKILGESIKAGATTVGFADTVGINMPQEFGELVAYVIENTPGADDI 288

Query: 287 IISTHCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEASYLFNLFPTYSFI 346
           + + HC NDLG+ATANT++G CAGARQVEVTINGIGER+GNA LEE              
Sbjct: 289 VFAIHCHNDLGVATANTISGICAGARQVEVTINGIGERSGNAPLEE-------------- 348

Query: 347 YFEIIIIQEGCVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGA 406
                      VVMAL+CRGE ++ G+++ I+SR I  TSKMV+E+TG+ VQPHK IVG 
Sbjct: 349 -----------VVMALKCRGESLMDGVYTKIDSRQIMATSKMVQEHTGMYVQPHKPIVGD 408

Query: 407 NAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYE 466
           N F HESGIHQDG+LK++ TYEI+SPED+G  +S ++GIVLGKLSGRHA+K RL+ELGYE
Sbjct: 409 NCFVHESGIHQDGILKNRSTYEILSPEDVGIVKSENSGIVLGKLSGRHAVKDRLKELGYE 468

Query: 467 LDNENLDNIFWRFKAVAEQKKRVTDADLRALV 496
           + +E  ++IF R++ + + KKR+TDADL+ALV
Sbjct: 469 ISDEKFNDIFSRYRELTKDKKRITDADLKALV 474

The following BLAST results are available for this feature:

BLAST of MC11g1352 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C550	2.1e-266	79.69	2-isopropylmalate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=I...	[more]
Q9LPR4	3.8e-263	77.89	2-isopropylmalate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=I...	[more]
O04973	6.7e-244	73.46	2-isopropylmalate synthase A OS=Solanum pennellii OX=28526 GN=IPMSA PE=2 SV=1	[more]
O04974	4.5e-240	72.81	2-isopropylmalate synthase B OS=Solanum pennellii OX=28526 GN=IPMSB PE=2 SV=1	[more]
Q39891	7.2e-214	66.49	Probable 2-isopropylmalate synthase OS=Glycine max OX=3847 GN=GMN56 PE=2 SV=1	[more]

BLAST of MC11g1352 vs. NCBI nr


Match Name	Identity	Description
XP_022141882.1	96.09	2-isopropylmalate synthase 2, chloroplastic-like isoform X2 [Momordica charantia...	[more]
XP_022141881.1	96.09	2-isopropylmalate synthase 2, chloroplastic-like isoform X1 [Momordica charantia...	[more]
XP_038875433.1	84.46	2-isopropylmalate synthase 2, chloroplastic-like [Benincasa hispida]	[more]
XP_011651428.1	82.57	2-isopropylmalate synthase 2, chloroplastic [Cucumis sativus] >KGN57885.1 hypoth...	[more]
TYK01483.1	82.92	2-isopropylmalate synthase 2 [Cucumis melo var. makuwa]	[more]

BLAST of MC11g1352 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1CL37	96.09	2-isopropylmalate synthase OS=Momordica charantia OX=3673 GN=LOC111012138 PE=3 S...	[more]
A0A6J1CKJ3	96.09	2-isopropylmalate synthase OS=Momordica charantia OX=3673 GN=LOC111012138 PE=3 S...	[more]
A0A0A0L7C8	82.57	2-isopropylmalate synthase OS=Cucumis sativus OX=3659 GN=Csa_3G363180 PE=3 SV=1	[more]
A0A5D3BP53	82.92	2-isopropylmalate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A1S3C4S6	82.54	2-isopropylmalate synthase OS=Cucumis melo OX=3656 GN=LOC103496846 PE=3 SV=1	[more]

BLAST of MC11g1352 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G74040.1	1.5e-267	79.69	2-isopropylmalate synthase 1	[more]
AT1G18500.1	2.7e-264	77.89	methylthioalkylmalate synthase-like 4	[more]
AT5G23010.1	1.7e-154	58.26	methylthioalkylmalate synthase 1	[more]
AT5G23020.1	1.1e-151	58.85	2-isopropylmalate synthase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013709	2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain	SMART	SM00917	LeuA_dimer_2	coord: 485..630 e-value: 1.4E-28 score: 111.0
IPR013709	2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain	PFAM	PF08502	LeuA_dimer	coord: 486..630 e-value: 2.7E-36 score: 124.4
IPR036230	2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain superfamily	GENE3D	3.30.160.270		coord: 515..630 e-value: 4.0E-24 score: 86.9
IPR036230	2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain superfamily	SUPERFAMILY	110921	2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain	coord: 509..629
IPR000891	Pyruvate carboxyltransferase	PFAM	PF00682	HMGL-like	coord: 82..330 e-value: 3.6E-89 score: 298.8
IPR000891	Pyruvate carboxyltransferase	PROSITE	PS50991	PYR_CT	coord: 82..349 score: 39.317265
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 69..346 e-value: 6.1E-100 score: 336.7
None	No IPR available	GENE3D	1.10.238.260		coord: 393..506 e-value: 1.1E-37 score: 130.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 58..79
None	No IPR available	PANTHER	PTHR10277:SF9	2-ISOPROPYLMALATE SYNTHASE 1, CHLOROPLASTIC	coord: 73..330 coord: 355..634
None	No IPR available	PANTHER	PTHR10277	HOMOCITRATE SYNTHASE-RELATED	coord: 73..330 coord: 355..634
None	No IPR available	CDD	cd07940	DRE_TIM_IPMS	coord: 84..388 e-value: 7.16156E-174 score: 493.119
None	No IPR available	SUPERFAMILY	51569	Aldolase	coord: 79..337
IPR002034	Alpha-isopropylmalate/homocitrate synthase, conserved site	PROSITE	PS00815	AIPM_HOMOCIT_SYNTH_1	coord: 89..105
IPR005671	2-isopropylmalate synthase, bacterial-type	HAMAP	MF_01025	LeuA_type1	coord: 79..639 score: 39.026711

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC11g1352.1	MC11g1352.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009098	leucine biosynthetic process
biological_process	GO:0019752	carboxylic acid metabolic process
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0003852	2-isopropylmalate synthase activity
molecular_function	GO:0046912	acyltransferase, acyl groups converted into alkyl on transfer
molecular_function	GO:0003824	catalytic activity