MC11g1302 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC11g1302
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionExonuclease domain-containing protein
LocationMC11: 15643480 .. 15643724 (-)
RNA-Seq ExpressionMC11g1302
SyntenyMC11g1302
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TGCAATGACATCACTAGAGATGTTGTCATTTCTATAGCCAATTTCGCTGAAATTTCTGATAGGGTTTACGATATCCTTCACAGACGGATATGGGCAGGCCATAACATACTGAGATTTGATTGTGCACGAATAAGGGAGGCTTTGGCAGAAATTGGTATGCCAGCACCAGAGCCGAAATGTACTATTGATTCATTATGGCATTGTTGACTAAAAAGTTTGGTAGGAGAGTTGGCGACATGAAGGTA

mRNA sequence

TGCAATGACATCACTAGAGATGTTGTCATTTCTATAGCCAATTTCGCTGAAATTTCTGATAGGGTTTACGATATCCTTCACAGACGGATATGGGCAGGCCATAACATACTGAGATTTGATTGTGCACGAATAAGGGAGGCTTTGGCAGAAATTGGTATGCCAGCACCAGAGCCGAAATGTACTATTGATTCATTATTGTTGACTAAAAAGTTTGGTAGGAGAGTTGGCGACATGAAGGTA

Coding sequence (CDS)

TGCAATGACATCACTAGAGATGTTGTCATTTCTATAGCCAATTTCGCTGAAATTTCTGATAGGGTTTACGATATCCTTCACAGACGGATATGGGCAGGCCATAACATACTGAGATTTGATTGTGCACGAATAAGGGAGGCTTTGGCAGAAATTGGTATGCCAGCACCAGAGCCGAAATGTACTATTGATTCATTATTGTTGACTAAAAAGTTTGGTAGGAGAGTTGGCGACATGAAGGTA

Protein sequence

CNDITRDVVISIANFAEISDRVYDILHRRIWAGHNILRFDCARIREALAEIGMPAPEPKCTIDSLLLTKKFGRRVGDMKV
Homology
BLAST of MC11g1302 vs. ExPASy Swiss-Prot
Match: Q9FLR0 (Protein NEN1 OS=Arabidopsis thaliana OX=3702 GN=NEN1 PE=2 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 3.2e-26
Identity = 59/81 (72.84%), Postives = 64/81 (79.01%), Query Frame = 0

Query: 1   CNDITRDVVISIANFAEISDRVYDILHRRIWAGHNILRFDCARIREALAEIGMPAPEPKC 60
           CN I RD V+    FA+I+D VYDILH RIWAGHNILRFDCARIREA AEIG   PEPK 
Sbjct: 65  CNGIKRDDVVLAPLFADIADTVYDILHGRIWAGHNILRFDCARIREAFAEIGRQPPEPKG 124

Query: 61  TIDSL-LLTKKFGRRVGDMKV 81
            IDSL LLT+KFGRR GDMK+
Sbjct: 125 AIDSLGLLTQKFGRRAGDMKM 145

BLAST of MC11g1302 vs. ExPASy Swiss-Prot
Match: Q9CA74 (Protein NEN3 OS=Arabidopsis thaliana OX=3702 GN=NEN3 PE=2 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 3.2e-26
Identity = 56/78 (71.79%), Postives = 66/78 (84.62%), Query Frame = 0

Query: 4   ITRDVVISIANFAEISDRVYDILHRRIWAGHNILRFDCARIREALAEIGMPAPEPKCTID 63
           ITRD V+S   F+EI+D+VYDILH RIWAGHNI+RFDC RIREA AEIG+  PEPK TID
Sbjct: 73  ITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIGLSPPEPKATID 132

Query: 64  SL-LLTKKFGRRVGDMKV 81
           SL LL++KFG+R GDMK+
Sbjct: 133 SLSLLSQKFGKRAGDMKM 150

BLAST of MC11g1302 vs. ExPASy Swiss-Prot
Match: Q0V842 (Protein NEN2 OS=Arabidopsis thaliana OX=3702 GN=NEN2 PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 2.1e-25
Identity = 57/81 (70.37%), Postives = 64/81 (79.01%), Query Frame = 0

Query: 1   CNDITRDVVISIANFAEISDRVYDILHRRIWAGHNILRFDCARIREALAEIGMPAPEPKC 60
           CN I R+ V S   FA+I+D VYDILH RIWAGHNIL+FDC RIREA AEIG   PEPK 
Sbjct: 73  CNGIKREDVESALTFADIADTVYDILHGRIWAGHNILKFDCPRIREAFAEIGRDPPEPKG 132

Query: 61  TIDSL-LLTKKFGRRVGDMKV 81
           TIDSL LLT++FGRR GDMK+
Sbjct: 133 TIDSLALLTQRFGRRAGDMKM 153

BLAST of MC11g1302 vs. ExPASy Swiss-Prot
Match: F4JJ23 (Protein NEN4 OS=Arabidopsis thaliana OX=3702 GN=NEN4 PE=2 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 3.2e-18
Identity = 43/78 (55.13%), Postives = 59/78 (75.64%), Query Frame = 0

Query: 4   ITRDVVISIANFAEISDRVYDILHRRIWAGHNILRFDCARIREALAEIGMPAPEPKCTID 63
           ITR  V +  +F +++++++ +L+ RIWAGHNI RFDC RI+EA AEIG  APEP   ID
Sbjct: 70  ITRAKVTNAPSFEDVAEKIHGLLNGRIWAGHNIRRFDCVRIKEAFAEIGKAAPEPSGIID 129

Query: 64  SL-LLTKKFGRRVGDMKV 81
           SL LL+ KFG+R G+MK+
Sbjct: 130 SLGLLSDKFGKRAGNMKM 147

BLAST of MC11g1302 vs. NCBI nr
Match: XP_022135360.1 (protein NEN1-like [Momordica charantia])

HSP 1 Score: 137 bits (344), Expect = 6.03e-36
Identity = 66/81 (81.48%), Postives = 72/81 (88.89%), Query Frame = 0

Query: 1   CNDITRDVVISIANFAEISDRVYDILHRRIWAGHNILRFDCARIREALAEIGMPAPEPKC 60
           CN ITRD V+S  NFA+I+DRVYDILH RIWAGHNILRFDCARIRE+ AEIGMPAPEPK 
Sbjct: 70  CNGITRDAVLSAPNFAQIADRVYDILHGRIWAGHNILRFDCARIRESFAEIGMPAPEPKG 129

Query: 61  TIDSL-LLTKKFGRRVGDMKV 80
           TIDSL LLT+KFGRR GDMK+
Sbjct: 130 TIDSLALLTQKFGRRAGDMKM 150

BLAST of MC11g1302 vs. NCBI nr
Match: XP_023515370.1 (protein NEN1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 136 bits (343), Expect = 9.78e-36
Identity = 67/81 (82.72%), Postives = 71/81 (87.65%), Query Frame = 0

Query: 1   CNDITRDVVISIANFAEISDRVYDILHRRIWAGHNILRFDCARIREALAEIGMPAPEPKC 60
           CN ITRD VIS   FA+I+DRVYDILH RIWAGHNILRFDCARIREA AEIGMPAPEPK 
Sbjct: 67  CNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAPEPKG 126

Query: 61  TIDSL-LLTKKFGRRVGDMKV 80
           TIDSL LLT+KFGRR GDMK+
Sbjct: 127 TIDSLALLTQKFGRRAGDMKM 147

BLAST of MC11g1302 vs. NCBI nr
Match: XP_023515369.1 (protein NEN1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 136 bits (343), Expect = 1.13e-35
Identity = 67/81 (82.72%), Postives = 71/81 (87.65%), Query Frame = 0

Query: 1   CNDITRDVVISIANFAEISDRVYDILHRRIWAGHNILRFDCARIREALAEIGMPAPEPKC 60
           CN ITRD VIS   FA+I+DRVYDILH RIWAGHNILRFDCARIREA AEIGMPAPEPK 
Sbjct: 67  CNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAPEPKG 126

Query: 61  TIDSL-LLTKKFGRRVGDMKV 80
           TIDSL LLT+KFGRR GDMK+
Sbjct: 127 TIDSLALLTQKFGRRAGDMKM 147

BLAST of MC11g1302 vs. NCBI nr
Match: XP_022921575.1 (protein NEN1-like [Cucurbita moschata] >KAG6589518.1 Protein NEN2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 135 bits (340), Expect = 2.63e-35
Identity = 66/81 (81.48%), Postives = 71/81 (87.65%), Query Frame = 0

Query: 1   CNDITRDVVISIANFAEISDRVYDILHRRIWAGHNILRFDCARIREALAEIGMPAPEPKC 60
           CN ITRD VIS   FA+I+DRVYDILH RIWAGHNILRFDCARIREA AEIGMPAP+PK 
Sbjct: 67  CNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAPQPKG 126

Query: 61  TIDSL-LLTKKFGRRVGDMKV 80
           TIDSL LLT+KFGRR GDMK+
Sbjct: 127 TIDSLALLTQKFGRRAGDMKM 147

BLAST of MC11g1302 vs. NCBI nr
Match: XP_022987423.1 (protein NEN1-like [Cucurbita maxima])

HSP 1 Score: 135 bits (340), Expect = 2.63e-35
Identity = 66/81 (81.48%), Postives = 71/81 (87.65%), Query Frame = 0

Query: 1   CNDITRDVVISIANFAEISDRVYDILHRRIWAGHNILRFDCARIREALAEIGMPAPEPKC 60
           CN ITRD VIS   FA+I+DRVYDILH RIWAGHNILRFDCARIREA AEIGMPAP+PK 
Sbjct: 67  CNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAPQPKG 126

Query: 61  TIDSL-LLTKKFGRRVGDMKV 80
           TIDSL LLT+KFGRR GDMK+
Sbjct: 127 TIDSLALLTQKFGRRAGDMKM 147

BLAST of MC11g1302 vs. ExPASy TrEMBL
Match: A0A6J1C0W8 (protein NEN1-like OS=Momordica charantia OX=3673 GN=LOC111007332 PE=4 SV=1)

HSP 1 Score: 137 bits (344), Expect = 2.92e-36
Identity = 66/81 (81.48%), Postives = 72/81 (88.89%), Query Frame = 0

Query: 1   CNDITRDVVISIANFAEISDRVYDILHRRIWAGHNILRFDCARIREALAEIGMPAPEPKC 60
           CN ITRD V+S  NFA+I+DRVYDILH RIWAGHNILRFDCARIRE+ AEIGMPAPEPK 
Sbjct: 70  CNGITRDAVLSAPNFAQIADRVYDILHGRIWAGHNILRFDCARIRESFAEIGMPAPEPKG 129

Query: 61  TIDSL-LLTKKFGRRVGDMKV 80
           TIDSL LLT+KFGRR GDMK+
Sbjct: 130 TIDSLALLTQKFGRRAGDMKM 150

BLAST of MC11g1302 vs. ExPASy TrEMBL
Match: A0A6J1JJE4 (protein NEN1-like OS=Cucurbita maxima OX=3661 GN=LOC111484975 PE=4 SV=1)

HSP 1 Score: 135 bits (340), Expect = 1.27e-35
Identity = 66/81 (81.48%), Postives = 71/81 (87.65%), Query Frame = 0

Query: 1   CNDITRDVVISIANFAEISDRVYDILHRRIWAGHNILRFDCARIREALAEIGMPAPEPKC 60
           CN ITRD VIS   FA+I+DRVYDILH RIWAGHNILRFDCARIREA AEIGMPAP+PK 
Sbjct: 67  CNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAPQPKG 126

Query: 61  TIDSL-LLTKKFGRRVGDMKV 80
           TIDSL LLT+KFGRR GDMK+
Sbjct: 127 TIDSLALLTQKFGRRAGDMKM 147

BLAST of MC11g1302 vs. ExPASy TrEMBL
Match: A0A6J1E665 (protein NEN1-like OS=Cucurbita moschata OX=3662 GN=LOC111429799 PE=4 SV=1)

HSP 1 Score: 135 bits (340), Expect = 1.27e-35
Identity = 66/81 (81.48%), Postives = 71/81 (87.65%), Query Frame = 0

Query: 1   CNDITRDVVISIANFAEISDRVYDILHRRIWAGHNILRFDCARIREALAEIGMPAPEPKC 60
           CN ITRD VIS   FA+I+DRVYDILH RIWAGHNILRFDCARIREA AEIGMPAP+PK 
Sbjct: 67  CNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAPQPKG 126

Query: 61  TIDSL-LLTKKFGRRVGDMKV 80
           TIDSL LLT+KFGRR GDMK+
Sbjct: 127 TIDSLALLTQKFGRRAGDMKM 147

BLAST of MC11g1302 vs. ExPASy TrEMBL
Match: A0A5A7UTA8 (Protein NEN1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002600 PE=4 SV=1)

HSP 1 Score: 133 bits (335), Expect = 5.52e-35
Identity = 65/81 (80.25%), Postives = 71/81 (87.65%), Query Frame = 0

Query: 1   CNDITRDVVISIANFAEISDRVYDILHRRIWAGHNILRFDCARIREALAEIGMPAPEPKC 60
           CN ITRD VIS   FA+I+DRVYDILH RIWAGHNILRFDCARIREA A+IG+PAPEPK 
Sbjct: 67  CNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFADIGVPAPEPKG 126

Query: 61  TIDSL-LLTKKFGRRVGDMKV 80
           TIDSL LLT++FGRR GDMKV
Sbjct: 127 TIDSLALLTQRFGRRAGDMKV 147

BLAST of MC11g1302 vs. ExPASy TrEMBL
Match: A0A0A0LSC3 (Exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G009910 PE=4 SV=1)

HSP 1 Score: 133 bits (335), Expect = 5.52e-35
Identity = 65/81 (80.25%), Postives = 71/81 (87.65%), Query Frame = 0

Query: 1   CNDITRDVVISIANFAEISDRVYDILHRRIWAGHNILRFDCARIREALAEIGMPAPEPKC 60
           CN ITRD VIS   FA+I+DRVYDILH RIWAGHNILRFDCARIREA AEIG+PAPEPK 
Sbjct: 67  CNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPEPKG 126

Query: 61  TIDSL-LLTKKFGRRVGDMKV 80
           TIDSL LLT++FGRR GDMK+
Sbjct: 127 TIDSLALLTQRFGRRAGDMKM 147

BLAST of MC11g1302 vs. TAIR 10
Match: AT1G74390.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 118.6 bits (296), Expect = 2.3e-27
Identity = 56/78 (71.79%), Postives = 66/78 (84.62%), Query Frame = 0

Query: 4   ITRDVVISIANFAEISDRVYDILHRRIWAGHNILRFDCARIREALAEIGMPAPEPKCTID 63
           ITRD V+S   F+EI+D+VYDILH RIWAGHNI+RFDC RIREA AEIG+  PEPK TID
Sbjct: 73  ITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIGLSPPEPKATID 132

Query: 64  SL-LLTKKFGRRVGDMKV 81
           SL LL++KFG+R GDMK+
Sbjct: 133 SLSLLSQKFGKRAGDMKM 150

BLAST of MC11g1302 vs. TAIR 10
Match: AT1G74390.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 118.6 bits (296), Expect = 2.3e-27
Identity = 56/78 (71.79%), Postives = 66/78 (84.62%), Query Frame = 0

Query: 4   ITRDVVISIANFAEISDRVYDILHRRIWAGHNILRFDCARIREALAEIGMPAPEPKCTID 63
           ITRD V+S   F+EI+D+VYDILH RIWAGHNI+RFDC RIREA AEIG+  PEPK TID
Sbjct: 73  ITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIGLSPPEPKATID 132

Query: 64  SL-LLTKKFGRRVGDMKV 81
           SL LL++KFG+R GDMK+
Sbjct: 133 SLSLLSQKFGKRAGDMKM 150

BLAST of MC11g1302 vs. TAIR 10
Match: AT5G07710.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 118.6 bits (296), Expect = 2.3e-27
Identity = 59/81 (72.84%), Postives = 64/81 (79.01%), Query Frame = 0

Query: 1   CNDITRDVVISIANFAEISDRVYDILHRRIWAGHNILRFDCARIREALAEIGMPAPEPKC 60
           CN I RD V+    FA+I+D VYDILH RIWAGHNILRFDCARIREA AEIG   PEPK 
Sbjct: 65  CNGIKRDDVVLAPLFADIADTVYDILHGRIWAGHNILRFDCARIREAFAEIGRQPPEPKG 124

Query: 61  TIDSL-LLTKKFGRRVGDMKV 81
            IDSL LLT+KFGRR GDMK+
Sbjct: 125 AIDSLGLLTQKFGRRAGDMKM 145

BLAST of MC11g1302 vs. TAIR 10
Match: AT5G61390.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 115.9 bits (289), Expect = 1.5e-26
Identity = 57/81 (70.37%), Postives = 64/81 (79.01%), Query Frame = 0

Query: 1   CNDITRDVVISIANFAEISDRVYDILHRRIWAGHNILRFDCARIREALAEIGMPAPEPKC 60
           CN I R+ V S   FA+I+D VYDILH RIWAGHNIL+FDC RIREA AEIG   PEPK 
Sbjct: 73  CNGIKREDVESALTFADIADTVYDILHGRIWAGHNILKFDCPRIREAFAEIGRDPPEPKG 132

Query: 61  TIDSL-LLTKKFGRRVGDMKV 81
           TIDSL LLT++FGRR GDMK+
Sbjct: 133 TIDSLALLTQRFGRRAGDMKM 153

BLAST of MC11g1302 vs. TAIR 10
Match: AT4G39810.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 92.0 bits (227), Expect = 2.3e-19
Identity = 43/78 (55.13%), Postives = 59/78 (75.64%), Query Frame = 0

Query: 4   ITRDVVISIANFAEISDRVYDILHRRIWAGHNILRFDCARIREALAEIGMPAPEPKCTID 63
           ITR  V +  +F +++++++ +L+ RIWAGHNI RFDC RI+EA AEIG  APEP   ID
Sbjct: 70  ITRAKVTNAPSFEDVAEKIHGLLNGRIWAGHNIRRFDCVRIKEAFAEIGKAAPEPSGIID 129

Query: 64  SL-LLTKKFGRRVGDMKV 81
           SL LL+ KFG+R G+MK+
Sbjct: 130 SLGLLSDKFGKRAGNMKM 147

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FLR03.2e-2672.84Protein NEN1 OS=Arabidopsis thaliana OX=3702 GN=NEN1 PE=2 SV=1[more]
Q9CA743.2e-2671.79Protein NEN3 OS=Arabidopsis thaliana OX=3702 GN=NEN3 PE=2 SV=1[more]
Q0V8422.1e-2570.37Protein NEN2 OS=Arabidopsis thaliana OX=3702 GN=NEN2 PE=2 SV=1[more]
F4JJ233.2e-1855.13Protein NEN4 OS=Arabidopsis thaliana OX=3702 GN=NEN4 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022135360.16.03e-3681.48protein NEN1-like [Momordica charantia][more]
XP_023515370.19.78e-3682.72protein NEN1-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023515369.11.13e-3582.72protein NEN1-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022921575.12.63e-3581.48protein NEN1-like [Cucurbita moschata] >KAG6589518.1 Protein NEN2, partial [Cucu... [more]
XP_022987423.12.63e-3581.48protein NEN1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1C0W82.92e-3681.48protein NEN1-like OS=Momordica charantia OX=3673 GN=LOC111007332 PE=4 SV=1[more]
A0A6J1JJE41.27e-3581.48protein NEN1-like OS=Cucurbita maxima OX=3661 GN=LOC111484975 PE=4 SV=1[more]
A0A6J1E6651.27e-3581.48protein NEN1-like OS=Cucurbita moschata OX=3662 GN=LOC111429799 PE=4 SV=1[more]
A0A5A7UTA85.52e-3580.25Protein NEN1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002600 ... [more]
A0A0A0LSC35.52e-3580.25Exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G009910... [more]
Match NameE-valueIdentityDescription
AT1G74390.12.3e-2771.79Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G74390.22.3e-2771.79Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G07710.12.3e-2772.84Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G61390.11.5e-2670.37Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT4G39810.12.3e-1955.13Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 1..78
e-value: 8.2E-8
score: 34.2
NoneNo IPR availablePANTHERPTHR30231DNA POLYMERASE III SUBUNIT EPSILONcoord: 1..80
NoneNo IPR availablePANTHERPTHR30231:SF27PROTEIN NEN3coord: 1..80
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 3..72

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC11g1302.1MC11g1302.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
molecular_function GO:0008408 3'-5' exonuclease activity
molecular_function GO:0003676 nucleic acid binding