MC11g1217 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC11g1217
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	subtilisin-like protease SBT3.3
Location	MC11: 12069466 .. 12071222 (+)
RNA-Seq Expression	MC11g1217
Synteny	MC11g1217

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GGAATTCTTTTTTTTTTAGATTACCACAAATTCAAACGTGAGAATTTGCACCAAACAATACAAACCGTCTCTAAAAATGATGATGGGATATATTTAGCTAAGGAAAAAAGAAAAACCAAAAATCCATAAAATCCAGCAGTGATGCCAGAGCCAGAGGAAGGTCAAGACATTGTACAAGAAGCGGGAGTCTCTCCCGCGATTTCATCTCCCTAAGGTCTCAAAATCTGTTCAGAGTCTTGATTCTCTGTCCAATTATCGTTCAACTTGGAATGGCAATGGCAATGGCGAATGCGAATTTTTCATCGTTTTGCATATATATTGTTGTGCTATGCTCGATTTCTGTGAATTTGGTTCTTAGCTCTGCGGCGATGCCCGGCAAATCCAAATCCGCTTCTCCGGCGGTGCATATCGTTTTCATGGAGAAGCCTCGCGGCGTGGAGCCTGAGGCCTATCACATCCAAACCCTAGCTTCGGTTCTCGGCAGGTATTTCTTCTCCTCTCTTCTCTTCTATTCTCCCCCTTCTTTGTGGAAATTGACGATGAATTTTGGTACAATCTGTTGATTTTCAGTGAAAAGGCTGCTAAAGAGGCTCTTGTGTATAGTTACAGCAATGCTGCCAGTGGATTCTCCGCTCGGCTTACTCCCGATCAGGTCGCGAAGATTTCCAGTAAGTCCCGCTCTTAGCATCATCTGCTTCAATTTCTTCGTCGTTTACCTATCATTGCTTGATCATCGTCTATTTAGTGGATTTCTGAAAGAATTTCATACATTGGAGCTGGGGATATGAAAATTTACTTCCTCATATGAAACGGATAATGGATGATATATTGAGCTTACTGTTATCTACATTTTTCTAGTTTTGTGCATTTCAAAGCGTCTTGAACCGCTAGTTTGCTTGGTTCGAGCAGGTTCTTGAAGTTGTCTGTTGATTATGCAATGAGTTAGATCAGGATTTTGATTGTCCCTTTACTCTGTCACATGTTTGCATTCCTGTTTGATGCTTGTTTGATCTTTCAATCTAAGTCGTAGTCTTCCTGGGTGGTATAAAAATAAAGGTTATATCATAAATTTAGTTAATAAGCTAGATGGTTGTGTCTATTTAATTTCTAAGCTTGATTGAATTTTGAACTGTGATTCTATGTAGTTCTTATTGTTTAGTGGGATTTCTTTGCATTTACTTTCCATATCTCTACAACTTATATATCCCAGGAGCATCGATAAGTTTCTCAAAACCGTCAATAACGTGTGTGGTGTATGCATATACATTTAGATTTTCATCTTAGAGTGATAAATCCTCACAGTTACCATAAGTTTTTTCTATTTTGGGACTTGGCAGAACAACCAGGAGTTTTAAATGTTGTGAACAGCGAAGCTCATGAGCTGCATGGTGGAGGAGCTGAAGGGCTTGACTAGAAGAACATCAAGTTCTTTCCCTGGAAATATTGGTGTGTCTGTTGCCTAACAAAAGTGAATTCTGTCTATATTGTGCATAATACCGAATACTCAAAGGAAAATACTCGGCTCCTCACTAGTAAGGAGGAACTTTGTCCTTACCATTGTTAAATATTTATCAACGCATCATCTTCTAAAAAATCTTTATGTTCTTGCATTTCTCTCTTAGTTGTCAAATATTTAAGGGTGGTGAGGACAGCGAGGAGCAAGTATTTTCCATACTCAAAATGTATCTATTTGCTATAATAATATGTGTTCATGTGCCTTTGGCCATTGATAATGAAGCTAATGATTTGCTGAGGAA

mRNA sequence

GGAATTCTTTTTTTTTTAGATTACCACAAATTCAAACGTGAGAATTTGCACCAAACAATACAAACCGTCTCTAAAAATGATGATGGGATATATTTAGCTAAGGAAAAAAGAAAAACCAAAAATCCATAAAATCCAGCAGTGATGCCAGAGCCAGAGGAAGGTCAAGACATTGTACAAGAAGCGGGAGTCTCTCCCGCGATTTCATCTCCCTAAGGTCTCAAAATCTGTTCAGAGTCTTGATTCTCTGTCCAATTATCGTTCAACTTGGAATGGCAATGGCAATGGCGAATGCGAATTTTTCATCGTTTTGCATATATATTGTTGTGCTATGCTCGATTTCTGTGAATTTGGTTCTTAGCTCTGCGGCGATGCCCGGCAAATCCAAATCCGCTTCTCCGGCGGTGCATATCGTTTTCATGGAGAAGCCTCGCGGCGTGGAGCCTGAGGCCTATCACATCCAAACCCTAGCTTCGGTTCTCGGCAGTGAAAAGGCTGCTAAAGAGGCTCTTGTGTATAGTTACAGCAATGCTGCCAGTGGATTCTCCGCTCGGCTTACTCCCGATCAGGTCGCGAAGATTTCCAAACAACCAGGAGTTTTAAATGTTGTGAACAGCGAAGCTCATGAGCTGCATGGTGGAGGAGCTGAAGGGCTTGACTAGAAGAACATCAAGTTCTTTCCCTGGAAATATTGGTGTGTCTGTTGCCTAACAAAAGTGAATTCTGTCTATATTGTGCATAATACCGAATACTCAAAGGAAAATACTCGGCTCCTCACTAGTAAGGAGGAACTTTGTCCTTACCATTGTTAAATATTTATCAACGCATCATCTTCTAAAAAATCTTTATGTTCTTGCATTTCTCTCTTAGTTGTCAAATATTTAAGGGTGGTGAGGACAGCGAGGAGCAAGTATTTTCCATACTCAAAATGTATCTATTTGCTATAATAATATGTGTTCATGTGCCTTTGGCCATTGATAATGAAGCTAATGATTTGCTGAGGAA

Coding sequence (CDS)

ATGGCAATGGCAATGGCGAATGCGAATTTTTCATCGTTTTGCATATATATTGTTGTGCTATGCTCGATTTCTGTGAATTTGGTTCTTAGCTCTGCGGCGATGCCCGGCAAATCCAAATCCGCTTCTCCGGCGGTGCATATCGTTTTCATGGAGAAGCCTCGCGGCGTGGAGCCTGAGGCCTATCACATCCAAACCCTAGCTTCGGTTCTCGGCAGTGAAAAGGCTGCTAAAGAGGCTCTTGTGTATAGTTACAGCAATGCTGCCAGTGGATTCTCCGCTCGGCTTACTCCCGATCAGGTCGCGAAGATTTCCAAACAACCAGGAGTTTTAAATGTTGTGAACAGCGAAGCTCATGAGCTGCATGGTGGAGGAGCTGAAGGGCTTGACTAG

Protein sequence

MAMAMANANFSSFCIYIVVLCSISVNLVLSSAAMPGKSKSASPAVHIVFMEKPRGVEPEAYHIQTLASVLGSEKAAKEALVYSYSNAASGFSARLTPDQVAKISKQPGVLNVVNSEAHELHGGGAEGLD

Homology

[Close]

BLAST of MC11g1217 vs. ExPASy Swiss-Prot
Match: Q9MAP5 (Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 4.4e-09
Identity = 40/106 (37.74%), Postives = 66/106 (62.26%), Query Frame = 0


Query: 18  VVLCSISVNLVLSSAAMPGKSKSASPAVHIVFMEKPRGVEPE---AYHIQTLASVLGSEK 77
           ++L  +S+  VL++     +++S    VHIV++ + +  +PE     H Q LAS+LGS+K
Sbjct: 8   ILLVLLSLITVLNATRARSETES---KVHIVYLGEKKHHDPEFVTESHHQMLASLLGSKK 67

Query: 78  AAKEALVYSYSNAASGFSARLTPDQVAKISKQPGVLNVVNSEAHEL 121
            A +++VYSY +  SGF+A+LT  Q  KI+  P V++V+    HEL
Sbjct: 68  DADDSMVYSYRHGFSGFAAKLTKSQAKKIADLPEVVHVIPDGFHEL 110

[Close]

BLAST of MC11g1217 vs. ExPASy Swiss-Prot
Match: F4HPF1 (Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 4.4e-09
Identity = 41/105 (39.05%), Postives = 64/105 (60.95%), Query Frame = 0


Query: 19  VLCSISVNLVLSSAAMPGKSKSASPAVHIVFMEKPRGVEPE---AYHIQTLASVLGSEKA 78
           VL  +S+ +VL+ A    KSK     VHIV++ + +  +P+     H Q L+S+LGS+  
Sbjct: 8   VLVVLSLIIVLNVARASAKSK-----VHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDD 67

Query: 79  AKEALVYSYSNAASGFSARLTPDQVAKISKQPGVLNVVNSEAHEL 121
           A E++VYSY +  SGF+A+LT  Q  KI+  P V++V+    +EL
Sbjct: 68  AHESMVYSYRHGFSGFAAKLTKSQAKKIADSPEVIHVIPDSYYEL 107

[Close]

BLAST of MC11g1217 vs. ExPASy Swiss-Prot
Match: Q1PDX5 (Subtilisin-like protease SBT3.11 OS=Arabidopsis thaliana OX=3702 GN=SBT3.11 PE=2 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.3e-08
Identity = 33/79 (41.77%), Postives = 51/79 (64.56%), Query Frame = 0


Query: 45  VHIVFMEKPRGVEPE---AYHIQTLASVLGSEKAAKEALVYSYSNAASGFSARLTPDQVA 104
           VHIV++ +    +PE   + H++ L S+LGS+K A E++V+SY N  SGF+A LT  Q  
Sbjct: 37  VHIVYLGEKEHNDPELVTSSHLRMLESLLGSKKDASESIVHSYRNGFSGFAAHLTDSQAE 96

Query: 105 KISKQPGVLNVVNSEAHEL 121
           +IS+ P V+ V  +  +EL
Sbjct: 97  QISEHPDVVQVTPNTFYEL 115

[Close]

BLAST of MC11g1217 vs. ExPASy Swiss-Prot
Match: Q9MAP7 (Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 1.4e-07
Identity = 37/104 (35.58%), Postives = 63/104 (60.58%), Query Frame = 0


Query: 21  CSISVNLVLSSAAMPGKSKSASPA-VHIVFMEKPRGVEPE---AYHIQTLASVLGSEKAA 80
           C + + LVLS   +    +++  + VHIV++ + +  +PE     H Q L+S+LGS+  A
Sbjct: 4   CRVLLVLVLSLVIVLNVVRASDESKVHIVYLGEKQHDDPEFVSESHHQMLSSLLGSKVDA 63

Query: 81  KEALVYSYSNAASGFSARLTPDQVAKISKQPGVLNVVNSEAHEL 121
            E++VYSY +  SGF+A+LT  Q  K++  P V++V+    +EL
Sbjct: 64  HESMVYSYRHGFSGFAAKLTESQAKKLADSPEVVHVMADSFYEL 107

[Close]

BLAST of MC11g1217 vs. ExPASy Swiss-Prot
Match: Q8L7I2 (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 2.4e-07
Identity = 39/109 (35.78%), Postives = 64/109 (58.72%), Query Frame = 0


Query: 15  IYIVVLCSISVNLVLSSAAMPGKSKSASPAVHIVFMEKPRGVEPE---AYHIQTLASVLG 74
           IY+V+   I +N+  S  A      SA   VHIV++ + +  +PE     H + L S+LG
Sbjct: 8   IYVVLSLVIFLNVQRSFVA----ESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLG 67

Query: 75  SEKAAKEALVYSYSNAASGFSARLTPDQVAKISKQPGVLNVVNSEAHEL 121
           S++ A +++VYSY +  SGF+A+LT  Q  KI+  P V++V+    ++L
Sbjct: 68  SKEDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKL 112

[Close]

BLAST of MC11g1217 vs. NCBI nr
Match: XP_022143651.1 (subtilisin-like protease SBT3.3 [Momordica charantia])

HSP 1 Score: 242 bits (618), Expect = 2.02e-80
Identity = 129/129 (100.00%), Postives = 129/129 (100.00%), Query Frame = 0


Query: 1   MAMAMANANFSSFCIYIVVLCSISVNLVLSSAAMPGKSKSASPAVHIVFMEKPRGVEPEA 60
           MAMAMANANFSSFCIYIVVLCSISVNLVLSSAAMPGKSKSASPAVHIVFMEKPRGVEPEA
Sbjct: 1   MAMAMANANFSSFCIYIVVLCSISVNLVLSSAAMPGKSKSASPAVHIVFMEKPRGVEPEA 60

Query: 61  YHIQTLASVLGSEKAAKEALVYSYSNAASGFSARLTPDQVAKISKQPGVLNVVNSEAHEL 120
           YHIQTLASVLGSEKAAKEALVYSYSNAASGFSARLTPDQVAKISKQPGVLNVVNSEAHEL
Sbjct: 61  YHIQTLASVLGSEKAAKEALVYSYSNAASGFSARLTPDQVAKISKQPGVLNVVNSEAHEL 120

Query: 121 HGGGAEGLD 129
           HGGGAEGLD
Sbjct: 121 HGGGAEGLD 129

[Close]

BLAST of MC11g1217 vs. NCBI nr
Match: XP_022143620.1 (subtilisin-like protease SBT3.11 isoform X1 [Momordica charantia])

HSP 1 Score: 157 bits (396), Expect = 1.21e-46
Identity = 86/120 (71.67%), Postives = 99/120 (82.50%), Query Frame = 0


Query: 5   MANANFSSFCIYIVVLCSISVNLVLSSAAMPGKSKSASPAVHIVFMEKPRGVEPEAYHIQ 64
           MA   FSSF + + +L SIS+N +LSSAAM G+   ASPAVHIV+ EKPR  EPEAYHI+
Sbjct: 1   MAKTTFSSFLLCLALLFSISLNFLLSSAAMSGQY--ASPAVHIVYTEKPRDEEPEAYHIR 60

Query: 65  TLASVLGSEKAAKEALVYSYSNAASGFSARLTPDQVAKISKQPGVLNVVNSEAHELHGGG 124
           TL SVLGSE+AAKEALVYSY NAASGFSARLTPDQVA++SKQPGVL+VV S  H+LH GG
Sbjct: 61  TLVSVLGSEEAAKEALVYSYKNAASGFSARLTPDQVAEVSKQPGVLHVVQSGTHQLHSGG 118

[Close]

BLAST of MC11g1217 vs. NCBI nr
Match: XP_022935866.1 (subtilisin-like protease SBT3.11 [Cucurbita moschata] >XP_023537186.1 subtilisin-like protease SBT3.11 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 149 bits (377), Expect = 9.98e-44
Identity = 85/124 (68.55%), Postives = 101/124 (81.45%), Query Frame = 0


Query: 5   MANANFSSFCIYIVVLCSISVNLVLSSAAMPGKS---KSASPAVHIVFMEKPRGVEPEAY 64
           MA   FSSF +  V+L SIS+NL+LSS+AM  +S    SASPAVHIV+MEKPR  EPEAY
Sbjct: 1   MAKLIFSSFPLCFVLLFSISLNLILSSSAMSAQSVDSASASPAVHIVYMEKPRDEEPEAY 60

Query: 65  HIQTLASVLGSEKAAKEALVYSYSNAASGFSARLTPDQVAKISKQPGVLNVVNSEAHELH 124
           HI+TLASVLGSE+AA+EALVYSY NAASGFSA+LTP+QVA++SKQ GVL VV S  ++LH
Sbjct: 61  HIRTLASVLGSEEAAREALVYSYKNAASGFSAKLTPNQVAEVSKQEGVLQVVPSRTYQLH 120

[Close]

BLAST of MC11g1217 vs. NCBI nr
Match: XP_038897393.1 (subtilisin-like protease SBT3.17 [Benincasa hispida])

HSP 1 Score: 148 bits (374), Expect = 2.52e-43
Identity = 85/122 (69.67%), Postives = 100/122 (81.97%), Query Frame = 0


Query: 5   MANANFSSFCIYIVVLCSISVNLVLSSAAMPGKS---KSASPAVHIVFMEKPRGVEPEAY 64
           MAN NFSS    +V+L S+S+NLVL+S+AM  +S    SASPAVHIV+MEKPR  EPEAY
Sbjct: 1   MANLNFSS----LVLLFSLSLNLVLTSSAMSAQSTDSNSASPAVHIVYMEKPRDEEPEAY 60

Query: 65  HIQTLASVLGSEKAAKEALVYSYSNAASGFSARLTPDQVAKISKQPGVLNVVNSEAHELH 123
           HI+TL SVLGSE+AA+EAL+YSY NAASGFSARLTPDQVA+ISKQ GVL VV S+  +LH
Sbjct: 61  HIRTLTSVLGSEEAAREALLYSYKNAASGFSARLTPDQVAEISKQEGVLQVVPSQTLQLH 118

[Close]

BLAST of MC11g1217 vs. NCBI nr
Match: XP_023534872.1 (subtilisin-like protease SBT3.6 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 142 bits (359), Expect = 8.37e-41
Identity = 83/131 (63.36%), Postives = 101/131 (77.10%), Query Frame = 0


Query: 5   MANANFSSFCIYIVVLCSISVNLVLSSAAMPGKSKS-------ASPAVHIVFMEKPRGVE 64
           M ++  SSF   +V+L SIS+NL LSSA M  +S S       ASPAVHIV+ E+P   E
Sbjct: 7   MKSSVSSSFPPILVLLFSISLNLFLSSAVMSDQSASIPVVSNFASPAVHIVYTERPHNEE 66

Query: 65  PEAYHIQTLASVLGSEKAAKEALVYSYSNAASGFSARLTPDQVAKISKQPGVLNVVNSEA 124
           PEAYHI+TLASVLGSE+AA+EALVYSY NAASGFSARLTP+QVA I+KQPGVL+VV S +
Sbjct: 67  PEAYHIRTLASVLGSEEAAREALVYSYKNAASGFSARLTPEQVADITKQPGVLHVVQSGS 126

Query: 125 HELHGGGAEGL 128
           ++LH GG  G+
Sbjct: 127 NKLHAGGGGGV 137

[Close]

BLAST of MC11g1217 vs. ExPASy TrEMBL
Match: A0A6J1CQX4 (subtilisin-like protease SBT3.3 OS=Momordica charantia OX=3673 GN=LOC111013512 PE=4 SV=1)

HSP 1 Score: 242 bits (618), Expect = 9.80e-81
Identity = 129/129 (100.00%), Postives = 129/129 (100.00%), Query Frame = 0


Query: 1   MAMAMANANFSSFCIYIVVLCSISVNLVLSSAAMPGKSKSASPAVHIVFMEKPRGVEPEA 60
           MAMAMANANFSSFCIYIVVLCSISVNLVLSSAAMPGKSKSASPAVHIVFMEKPRGVEPEA
Sbjct: 1   MAMAMANANFSSFCIYIVVLCSISVNLVLSSAAMPGKSKSASPAVHIVFMEKPRGVEPEA 60

Query: 61  YHIQTLASVLGSEKAAKEALVYSYSNAASGFSARLTPDQVAKISKQPGVLNVVNSEAHEL 120
           YHIQTLASVLGSEKAAKEALVYSYSNAASGFSARLTPDQVAKISKQPGVLNVVNSEAHEL
Sbjct: 61  YHIQTLASVLGSEKAAKEALVYSYSNAASGFSARLTPDQVAKISKQPGVLNVVNSEAHEL 120

Query: 121 HGGGAEGLD 129
           HGGGAEGLD
Sbjct: 121 HGGGAEGLD 129

[Close]

BLAST of MC11g1217 vs. ExPASy TrEMBL
Match: A0A6J1CPA2 (subtilisin-like protease SBT3.11 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013481 PE=4 SV=1)

HSP 1 Score: 157 bits (396), Expect = 5.83e-47
Identity = 86/120 (71.67%), Postives = 99/120 (82.50%), Query Frame = 0


Query: 5   MANANFSSFCIYIVVLCSISVNLVLSSAAMPGKSKSASPAVHIVFMEKPRGVEPEAYHIQ 64
           MA   FSSF + + +L SIS+N +LSSAAM G+   ASPAVHIV+ EKPR  EPEAYHI+
Sbjct: 1   MAKTTFSSFLLCLALLFSISLNFLLSSAAMSGQY--ASPAVHIVYTEKPRDEEPEAYHIR 60

Query: 65  TLASVLGSEKAAKEALVYSYSNAASGFSARLTPDQVAKISKQPGVLNVVNSEAHELHGGG 124
           TL SVLGSE+AAKEALVYSY NAASGFSARLTPDQVA++SKQPGVL+VV S  H+LH GG
Sbjct: 61  TLVSVLGSEEAAKEALVYSYKNAASGFSARLTPDQVAEVSKQPGVLHVVQSGTHQLHSGG 118

[Close]

BLAST of MC11g1217 vs. ExPASy TrEMBL
Match: A0A6J1F5X6 (subtilisin-like protease SBT3.11 OS=Cucurbita moschata OX=3662 GN=LOC111442644 PE=4 SV=1)

HSP 1 Score: 149 bits (377), Expect = 4.83e-44
Identity = 85/124 (68.55%), Postives = 101/124 (81.45%), Query Frame = 0


Query: 5   MANANFSSFCIYIVVLCSISVNLVLSSAAMPGKS---KSASPAVHIVFMEKPRGVEPEAY 64
           MA   FSSF +  V+L SIS+NL+LSS+AM  +S    SASPAVHIV+MEKPR  EPEAY
Sbjct: 1   MAKLIFSSFPLCFVLLFSISLNLILSSSAMSAQSVDSASASPAVHIVYMEKPRDEEPEAY 60

Query: 65  HIQTLASVLGSEKAAKEALVYSYSNAASGFSARLTPDQVAKISKQPGVLNVVNSEAHELH 124
           HI+TLASVLGSE+AA+EALVYSY NAASGFSA+LTP+QVA++SKQ GVL VV S  ++LH
Sbjct: 61  HIRTLASVLGSEEAAREALVYSYKNAASGFSAKLTPNQVAEVSKQEGVLQVVPSRTYQLH 120

[Close]

BLAST of MC11g1217 vs. ExPASy TrEMBL
Match: A0A6J1F6S6 (subtilisin-like protease SBT3.13 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442643 PE=4 SV=1)

HSP 1 Score: 140 bits (352), Expect = 4.55e-40
Identity = 82/127 (64.57%), Postives = 99/127 (77.95%), Query Frame = 0


Query: 5   MANANFSSFCIYIVVLCSISVNLVLSSAAMPGKSKS-------ASPAVHIVFMEKPRGVE 64
           M ++  SSF   +V+L SIS+NL LSSA M  +S S       ASPAVHIV+ E+P   E
Sbjct: 7   MKSSVSSSFPPILVLLFSISLNLFLSSAVMSDQSASIPVVSNFASPAVHIVYTERPHNEE 66

Query: 65  PEAYHIQTLASVLGSEKAAKEALVYSYSNAASGFSARLTPDQVAKISKQPGVLNVVNSEA 124
           PEAYHI+TLASVLGSE+AA+EALVYSY NAASGFSARLTP+QVA I+KQPGVL+VV S +
Sbjct: 67  PEAYHIRTLASVLGSEEAAREALVYSYKNAASGFSARLTPEQVADITKQPGVLHVVQSGS 126

[Close]

BLAST of MC11g1217 vs. ExPASy TrEMBL
Match: A0A6J1IH38 (subtilisin-like protease SBT3.6 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477163 PE=4 SV=1)

HSP 1 Score: 138 bits (347), Expect = 2.70e-39
Identity = 81/131 (61.83%), Postives = 99/131 (75.57%), Query Frame = 0


Query: 5   MANANFSSFCIYIVVLCSISVNLVLSSAAMPGKSKS-------ASPAVHIVFMEKPRGVE 64
           M ++  SSF   +V + SI +NL LSSA M  +S S       ASPAVHIV+ E+P   E
Sbjct: 7   MKSSVSSSFPPILVFIFSIYLNLFLSSAVMSDQSASIPVVSNFASPAVHIVYTERPHNEE 66

Query: 65  PEAYHIQTLASVLGSEKAAKEALVYSYSNAASGFSARLTPDQVAKISKQPGVLNVVNSEA 124
           PEAYHI+TLASVLGSE+AA+EALVYSY NAASGFSARLTP+QVA I+KQPGVL+VV S +
Sbjct: 67  PEAYHIRTLASVLGSEEAAREALVYSYKNAASGFSARLTPEQVADITKQPGVLHVVQSGS 126

Query: 125 HELHGGGAEGL 128
           ++LH GG  G+
Sbjct: 127 NKLHTGGGGGV 137

[Close]

BLAST of MC11g1217 vs. TAIR 10
Match: AT1G71950.1 (Proteinase inhibitor, propeptide )

HSP 1 Score: 109.4 bits (272), Expect = 2.2e-24
Identity = 56/112 (50.00%), Postives = 79/112 (70.54%), Query Frame = 0


Query: 16  YIVVLCSISVNLVLSSAAMPGKSKSASPAVHIVFMEKPRGVEPEAYHIQTLASVLGSEKA 75
           +   L S   ++V++       + S+   VHI++ EKP   EP+ YH++TL+S LGSE+A
Sbjct: 20  FFFCLSSFFASIVMADEYTGEATGSSEAKVHIIYTEKPTDEEPKTYHLRTLSSALGSEEA 79

Query: 76  AKEALVYSYSNAASGFSARLTPDQVAKISKQPGVLNVVNSEAHELHGGGAEG 128
           AK+AL+YSY  AASGFSA+LTP+QVA+ISKQPGV+ VV S+ ++LH  G  G
Sbjct: 80  AKDALIYSYKEAASGFSAKLTPEQVAEISKQPGVIQVVPSQTYQLHKPGGGG 131

[Close]

BLAST of MC11g1217 vs. TAIR 10
Match: AT1G32960.1 (Subtilase family protein )

HSP 1 Score: 62.4 bits (150), Expect = 3.1e-10
Identity = 40/106 (37.74%), Postives = 66/106 (62.26%), Query Frame = 0


Query: 18  VVLCSISVNLVLSSAAMPGKSKSASPAVHIVFMEKPRGVEPE---AYHIQTLASVLGSEK 77
           ++L  +S+  VL++     +++S    VHIV++ + +  +PE     H Q LAS+LGS+K
Sbjct: 8   ILLVLLSLITVLNATRARSETES---KVHIVYLGEKKHHDPEFVTESHHQMLASLLGSKK 67

Query: 78  AAKEALVYSYSNAASGFSARLTPDQVAKISKQPGVLNVVNSEAHEL 121
            A +++VYSY +  SGF+A+LT  Q  KI+  P V++V+    HEL
Sbjct: 68  DADDSMVYSYRHGFSGFAAKLTKSQAKKIADLPEVVHVIPDGFHEL 110

[Close]

BLAST of MC11g1217 vs. TAIR 10
Match: AT1G32950.1 (Subtilase family protein )

HSP 1 Score: 62.4 bits (150), Expect = 3.1e-10
Identity = 41/105 (39.05%), Postives = 64/105 (60.95%), Query Frame = 0


Query: 19  VLCSISVNLVLSSAAMPGKSKSASPAVHIVFMEKPRGVEPE---AYHIQTLASVLGSEKA 78
           VL  +S+ +VL+ A    KSK     VHIV++ + +  +P+     H Q L+S+LGS+  
Sbjct: 8   VLVVLSLIIVLNVARASAKSK-----VHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDD 67

Query: 79  AKEALVYSYSNAASGFSARLTPDQVAKISKQPGVLNVVNSEAHEL 121
           A E++VYSY +  SGF+A+LT  Q  KI+  P V++V+    +EL
Sbjct: 68  AHESMVYSYRHGFSGFAAKLTKSQAKKIADSPEVIHVIPDSYYEL 107

[Close]

BLAST of MC11g1217 vs. TAIR 10
Match: AT5G11940.1 (Subtilase family protein )

HSP 1 Score: 60.8 bits (146), Expect = 9.0e-10
Identity = 33/79 (41.77%), Postives = 51/79 (64.56%), Query Frame = 0


Query: 45  VHIVFMEKPRGVEPE---AYHIQTLASVLGSEKAAKEALVYSYSNAASGFSARLTPDQVA 104
           VHIV++ +    +PE   + H++ L S+LGS+K A E++V+SY N  SGF+A LT  Q  
Sbjct: 37  VHIVYLGEKEHNDPELVTSSHLRMLESLLGSKKDASESIVHSYRNGFSGFAAHLTDSQAE 96

Query: 105 KISKQPGVLNVVNSEAHEL 121
           +IS+ P V+ V  +  +EL
Sbjct: 97  QISEHPDVVQVTPNTFYEL 115

[Close]

BLAST of MC11g1217 vs. TAIR 10
Match: AT1G32940.1 (Subtilase family protein )

HSP 1 Score: 57.4 bits (137), Expect = 1.0e-08
Identity = 37/104 (35.58%), Postives = 63/104 (60.58%), Query Frame = 0


Query: 21  CSISVNLVLSSAAMPGKSKSASPA-VHIVFMEKPRGVEPE---AYHIQTLASVLGSEKAA 80
           C + + LVLS   +    +++  + VHIV++ + +  +PE     H Q L+S+LGS+  A
Sbjct: 4   CRVLLVLVLSLVIVLNVVRASDESKVHIVYLGEKQHDDPEFVSESHHQMLSSLLGSKVDA 63

Query: 81  KEALVYSYSNAASGFSARLTPDQVAKISKQPGVLNVVNSEAHEL 121
            E++VYSY +  SGF+A+LT  Q  K++  P V++V+    +EL
Sbjct: 64  HESMVYSYRHGFSGFAAKLTESQAKKLADSPEVVHVMADSFYEL 107

The following BLAST results are available for this feature:

BLAST of MC11g1217 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9MAP5	4.4e-09	37.74	Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 S...	[more]
F4HPF1	4.4e-09	39.05	Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 S...	[more]
Q1PDX5	1.3e-08	41.77	Subtilisin-like protease SBT3.11 OS=Arabidopsis thaliana OX=3702 GN=SBT3.11 PE=2...	[more]
Q9MAP7	1.4e-07	35.58	Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 S...	[more]
Q8L7I2	2.4e-07	35.78	Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 S...	[more]

BLAST of MC11g1217 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143651.1	2.02e-80	100.00	subtilisin-like protease SBT3.3 [Momordica charantia]	[more]
XP_022143620.1	1.21e-46	71.67	subtilisin-like protease SBT3.11 isoform X1 [Momordica charantia]	[more]
XP_022935866.1	9.98e-44	68.55	subtilisin-like protease SBT3.11 [Cucurbita moschata] >XP_023537186.1 subtilisin...	[more]
XP_038897393.1	2.52e-43	69.67	subtilisin-like protease SBT3.17 [Benincasa hispida]	[more]
XP_023534872.1	8.37e-41	63.36	subtilisin-like protease SBT3.6 isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC11g1217 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CQX4	9.80e-81	100.00	subtilisin-like protease SBT3.3 OS=Momordica charantia OX=3673 GN=LOC111013512 P...	[more]
A0A6J1CPA2	5.83e-47	71.67	subtilisin-like protease SBT3.11 isoform X1 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1F5X6	4.83e-44	68.55	subtilisin-like protease SBT3.11 OS=Cucurbita moschata OX=3662 GN=LOC111442644 P...	[more]
A0A6J1F6S6	4.55e-40	64.57	subtilisin-like protease SBT3.13 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1IH38	2.70e-39	61.83	subtilisin-like protease SBT3.6 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]

BLAST of MC11g1217 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G71950.1	2.2e-24	50.00	Proteinase inhibitor, propeptide	[more]
AT1G32960.1	3.1e-10	37.74	Subtilase family protein	[more]
AT1G32950.1	3.1e-10	39.05	Subtilase family protein	[more]
AT5G11940.1	9.0e-10	41.77	Subtilase family protein	[more]
AT1G32940.1	1.0e-08	35.58	Subtilase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 46..120 e-value: 4.1E-12 score: 46.6
IPR037045	Peptidase S8 propeptide/proteinase inhibitor I9 superfamily	GENE3D	3.30.70.80	Peptidase S8 propeptide/proteinase inhibitor I9	coord: 28..121 e-value: 4.5E-23 score: 83.5
None	No IPR available	PANTHER	PTHR48222	PROTEINASE INHIBITOR, PROPEPTIDE	coord: 23..123

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC11g1217.1	MC11g1217.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0008233	peptidase activity