MC11g1041 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC11g1041
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionmeiotic recombination protein SPO11-1
LocationMC11: 8945555 .. 8949095 (+)
RNA-Seq ExpressionMC11g1041
SyntenyMC11g1041
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGGAAAGCGAGCAAGCTCGGAGAGAAGCGACATTCTAAGAAGAATCAGAGGTTCCAATTCCATTCAATTTTCTCATTATCATATTGGTTCCAAACAATTACTGATTCGATTGTTGCTATTTTCCTTCCGCTTTTTAATTTCAACGTTTTTCAGAGATAACAAGATCTATGATCGTCGAGCTGAGCAACGGAAGCTCCAAGATGATGGCAATCGATCGGTTTAAAAATTACTGCACTAATTCTCTTGAAAACTGGTTCGGATCTTGTTTCGTTTGGTCGCAGTTTTGGTTTCTACTGAACCTGCAAATTAGCTACCAAATAAAGATCATAATCTATTACGATTCTATGTGATTTACTCGACTAGGAATGTTCATTTCCCAATCTGTATTCATTTTTGAATCATTTAGGATTCGTTATTTACTGCTGAGTTTCTTGGATCTTCCCGGAGGCCAGAACAGTTATCGTTTGTAAAATCCAGCCAAAAAAAAAAAAACCTAGTAGCAGTTCGGACGAGATTTTATCACTCGAAATTGAGGCACAAAGTTGCATAGTTCAAATCAGCATTTAACATTTACCACCTAGTTTTCAGTTTCGTGAAACACATTATGAGTTTTTAAGAATAATGTTTCTACTTGTCAATACAATGTATTTCTGGAACGTGAAGAGGTAGATCTTGAATTTGGACTTTAAATGCAGCTGTTGCAAGTTCAATTTATCCGAAGGGAAGGAGATTCTAACACTTCAAAAGGAAAGCCATGTCCGTAGACTAGGTGATTCAGTTATGTCAATGAATGATATTATAATCTGTATAAAGTACACCTGTGTCTTATTTGATCTTATTTTGCTCTATCAGATGTCTTGCTTAGAGTTCTACTCATTGTTCGGCAGCTTCTGCGAGAAAATAGACATAGCTCGAAGAGAGATATATATTATATGCATCCCTCTGTGTTTTCAGGTAAAGCTAACCCATCATTTTATCAGCAGAAACCAGTAGTTTTGTTCCAAGATACAATATCTTTCACAAAATAATAAGCACTCAAGTTCGGAAGGATCGGAATTCAACATATCCTTTAAATTAAAGTTTTCAGTAAGTTCGTGGCAGGAGATTTTCTCAACAATGCTTTGTATAGCATGCCAGATTTTATTGACATTGGCTTCTTCAAATAATATTTGCAGATCAGTCAGTTGTTGATCGGGCAATCAACGACATCTGCATTCTTTTGCAATGTAGTCGGCACAATCTAAATGTGGTTGGTGCCGCACTTCATTTTACTGTTTATTCAATTTGTGCTTTTTTTTTTCTTTTTTTCAATGATTATATAATGTGATTTATGGGGGATCTAATTTCTGTATGGTACTACTAACGGTTGGCTGCATATCTTAAATTGGCCGCATATTTTAGCTTTAATAAATAAACTTACAGTTGGTTTTTTGCTTTCAAACATCACTAACAGTTCAATTACAGTTTTCTTTAAGTTCCGTAGTTTGTGTCGCAAGCTTCTTCTGATAATAAAGTGTGTGATGATCATTTCAACTTGTGTCCTCTTGACAGGTTTCTTTTGGAAATGGGTACGTATTGAAGATATAATATGCTCAGTTCAGATAAGAAGAAAATAGTGACGAATGCTGTTTGTTTTCTTGACATGAGCTTTTACAAGCAGTCTGTATCACATAATTCTTTGTTATGTGGGTTGATACCTACCCATGTCAGGATTTTCTAAACCATTAGATTCTGTTAAAGAAAAACTTGTTGTTTTTGCTGCGCTCTGTTCCATTGAGGAAGAAAATTAAGAACTGAATCTTGACTTAGACACTTCTTTATAGGTTAGTGATGGGTTGGTTGAGGTTCTCAGAAGCTGGTAGGAGATTTGATTGCATAAAGAAGCCTAACAGTGTAAGCATTATACTTGAACTTGATTGTTCTTCATGATGCAATTGCTTCTTTTTTTAACTCTTAGCTGTACGTAATTTGGATATATGCATCTCAATCACTGCCAGGCCCACTCCATTCCTGTTCAAGTTGAGGAAGTCACAGGTATGCAGATACTGATTGATAAATCGCCAATCCACTGCGTTAGAACTTAAAATAGAAACTTTCTGAATGATATCTTTGGATATGGATTTCTATGCTGTATTCTGCCAAAGTTCGACATAAAATTACATGGTTTTTATCGTATTCCTCATAATCTTCAAAACTTTTGGCCTGAGATTCTGCTTTAGCTTGTTATTATTTTATTCTCATCTGCAGACATTGTTAGTGCTGCGGAATACATACTCATCGTTGAGAAGGAATCAGGTGAAGGTTATAGTGATAGAAGCTAGTTTTGCGGTTTAAATAAACTCAATTTTAAGTTTGACCTCGATCAGTCAATATATATGGTTTGATGTTTTCAATGTGCAGTTTTCCAGCGTTTAGCAAATGACCAGTTCTGCAGCAGAAACCGCTGCATTGTAATCACGGTGAGTATGTCTCTGGTCCTGCAACTTTGAAATTAGTTCAACAATAGGGTGGGGATTTGAACCTCCAAAATTTTGTTCATCAATGCATGCTTTAACTAGTCGAGTTACACTCGGTTAACTTGAAAATTCTCATATGTAAATGTTAGTTTTCATTTTCATTTTCAGGGACGTGGATATCCAGATGTTCCCACCAGAAGGTAAAGCAAAATATGTTCATACACACACATTTTTGGATGCTAATCTCATTTTACTGTTGGTGATGATTATATTATATGAATCATGGGAATATGCATGTATTAGTCAGGTTTTTGCGGCTTCTCGTTGATGTTTCGGCATTGCCTGCATTTTGCTTGGTAGATTGTGATCCATATGGTTTCGAAATTCTAACAACATATCGATTTGGTTCCATGGTTAGGCTCCTAAAACAATCATCTAAATCATTTATCTACTCAATTATGTTAAGAGAGCAGTGAACTAATCGACTATGTGGAACAATGAGACATTGAGAAGCATTTTTTTTTTTTCAGTTAAGGCTAAACTAACTTCCAGTTAGTGATTTTGACCCGTTTCCTTTTCACTAGCAAATGGCGTACGATGCAAAGTATCTCAGAATTCCACATCTACGCTGGCTTGGTGCTTTTGCTTCAGACTCTGAGAATTACAATCTTCCAGAACAATGTCTCATCCCATTGACTATGGAGGGTGAGGAATAATATATTCTAATACTCTCTATCTTTGCGGTTTCAAATGAAATTTTTCTGACCTTTTCTGAGGGTCTTGCTTATCCTGTCAGATAAGAGAAGAACCGAAGCCTTGTTACTCAGATGCTACTTGCAGAGAGAGGTACCACATTGGAGGTAAGCTTCTGTTAAGAACTCTAATTTTCTGTTGTCCTTGTCTTCATGCATATTGCCATTTTCTACAAAGAGGATTTGATCCCTTAGGTTGGAATTGGACTCAATGCTGCAAAAGGGTGTGAAATTTGAGATAGAAGCCCTCTCTGTGCACAATCTTTCCTTTTTGTCAGAAGAGTATTTACCATCCAAAATAGGATGTGTTCATCTTAGAACTGGTAAA

mRNA sequence

ATGGAGGGAAAGCGAGCAAGCTCGGAGAGAAGCGACATTCTAAGAAGAATCAGAGAGATAACAAGATCTATGATCGTCGAGCTGAGCAACGGAAGCTCCAAGATGATGGCAATCGATCGGTTTAAAAATTACTGCACTAATTCTCTTGAAAACTGCTGTTGCAAGTTCAATTTATCCGAAGGGAAGGAGATTCTAACACTTCAAAAGGAAAGCCATGTCCGTAGACTAGATGTCTTGCTTAGAGTTCTACTCATTGTTCGGCAGCTTCTGCGAGAAAATAGACATAGCTCGAAGAGAGATATATATTATATGCATCCCTCTGTGTTTTCAGATCAGTCAGTTGTTGATCGGGCAATCAACGACATCTGCATTCTTTTGCAATGTAGTCGGCACAATCTAAATGTGGTTTCTTTTGGAAATGGGTTAGTGATGGGTTGGTTGAGGTTCTCAGAAGCTGGTAGGAGATTTGATTGCATAAAGAAGCCTAACAGTGCCCACTCCATTCCTGTTCAAGTTGAGGAAGTCACAGACATTGTTAGTGCTGCGGAATACATACTCATCGTTGAGAAGGAATCAGTTTTCCAGCGTTTAGCAAATGACCAGTTCTGCAGCAGAAACCGCTGCATTGTAATCACGGGACGTGGATATCCAGATGTTCCCACCAGAAGGTTTTTGCGGCTTCTCGTTGATGTTTCGGCATTGCCTGCATTTTGCTTGGTAGATTGTGATCCATATGGTTTCGAAATTCTAACAACATATCGATTTGGTTCCATGCAAATGGCGTACGATGCAAAGTATCTCAGAATTCCACATCTACGCTGGCTTGGTGCTTTTGCTTCAGACTCTGAGAATTACAATCTTCCAGAACAATGTCTCATCCCATTGACTATGGAGGATAAGAGAAGAACCGAAGCCTTGTTACTCAGATGCTACTTGCAGAGAGAGGTACCACATTGGAGGTTGGAATTGGACTCAATGCTGCAAAAGGGTGTGAAATTTGAGATAGAAGCCCTCTCTGTGCACAATCTTTCCTTTTTGTCAGAAGAGTATTTACCATCCAAAATAGGATGTGTTCATCTTAGAACTGGTAAA

Coding sequence (CDS)

ATGGAGGGAAAGCGAGCAAGCTCGGAGAGAAGCGACATTCTAAGAAGAATCAGAGAGATAACAAGATCTATGATCGTCGAGCTGAGCAACGGAAGCTCCAAGATGATGGCAATCGATCGGTTTAAAAATTACTGCACTAATTCTCTTGAAAACTGCTGTTGCAAGTTCAATTTATCCGAAGGGAAGGAGATTCTAACACTTCAAAAGGAAAGCCATGTCCGTAGACTAGATGTCTTGCTTAGAGTTCTACTCATTGTTCGGCAGCTTCTGCGAGAAAATAGACATAGCTCGAAGAGAGATATATATTATATGCATCCCTCTGTGTTTTCAGATCAGTCAGTTGTTGATCGGGCAATCAACGACATCTGCATTCTTTTGCAATGTAGTCGGCACAATCTAAATGTGGTTTCTTTTGGAAATGGGTTAGTGATGGGTTGGTTGAGGTTCTCAGAAGCTGGTAGGAGATTTGATTGCATAAAGAAGCCTAACAGTGCCCACTCCATTCCTGTTCAAGTTGAGGAAGTCACAGACATTGTTAGTGCTGCGGAATACATACTCATCGTTGAGAAGGAATCAGTTTTCCAGCGTTTAGCAAATGACCAGTTCTGCAGCAGAAACCGCTGCATTGTAATCACGGGACGTGGATATCCAGATGTTCCCACCAGAAGGTTTTTGCGGCTTCTCGTTGATGTTTCGGCATTGCCTGCATTTTGCTTGGTAGATTGTGATCCATATGGTTTCGAAATTCTAACAACATATCGATTTGGTTCCATGCAAATGGCGTACGATGCAAAGTATCTCAGAATTCCACATCTACGCTGGCTTGGTGCTTTTGCTTCAGACTCTGAGAATTACAATCTTCCAGAACAATGTCTCATCCCATTGACTATGGAGGATAAGAGAAGAACCGAAGCCTTGTTACTCAGATGCTACTTGCAGAGAGAGGTACCACATTGGAGGTTGGAATTGGACTCAATGCTGCAAAAGGGTGTGAAATTTGAGATAGAAGCCCTCTCTGTGCACAATCTTTCCTTTTTGTCAGAAGAGTATTTACCATCCAAAATAGGATGTGTTCATCTTAGAACTGGTAAA

Protein sequence

MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSEGKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAINDICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVSAAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLVDCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDKRRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKIGCVHLRTGK
Homology
BLAST of MC11g1041 vs. ExPASy Swiss-Prot
Match: Q9M4A2 (Meiotic recombination protein SPO11-1 OS=Arabidopsis thaliana OX=3702 GN=SPO11-1 PE=1 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 2.6e-144
Identity = 245/355 (69.01%), Postives = 300/355 (84.51%), Query Frame = 0

Query: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60
           MEGK A SE +++L+RI++ T+S++V+L+ G S  ++I++F+NYC N   +C C  +  +
Sbjct: 1   MEGKFAISESTNLLQRIKDFTQSVVVDLAEGRSPKISINQFRNYCMNPEADCLCSSDKPK 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120
           G+EI TL+KE    R+D+LLRVLLIV+QLL+ENRH+SKRDIYYMHPS F  QS+VDRAI 
Sbjct: 61  GQEIFTLKKEPQTYRIDMLLRVLLIVQQLLQENRHASKRDIYYMHPSAFKAQSIVDRAIG 120

Query: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS 180
           DICIL QCSR+NLNVVS GNGLVMGWL+F EAGR+FDC+   N+A+ +PV VEEV DIVS
Sbjct: 121 DICILFQCSRYNLNVVSVGNGLVMGWLKFREAGRKFDCLNSLNTAYPVPVLVEEVEDIVS 180

Query: 181 AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV 240
            AEYIL+VEKE+VFQRLAND FC  NRCIVITGRGYPDV TRRFLRLL++   LP  CLV
Sbjct: 181 LAEYILVVEKETVFQRLANDMFCKTNRCIVITGRGYPDVSTRRFLRLLMEKLHLPVHCLV 240

Query: 241 DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK 300
           DCDPYGFEIL TYRFGSMQMAYD + LR P ++WLGAF SDSE Y++P+QCL+PLT EDK
Sbjct: 241 DCDPYGFEILATYRFGSMQMAYDIESLRAPDMKWLGAFPSDSEVYSVPKQCLLPLTEEDK 300

Query: 301 RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKI 356
           +RTEA+LLRCYL+RE+P WRLEL++ML++GVKFEIEALSVH+LSFLSE Y+PSKI
Sbjct: 301 KRTEAMLLRCYLKREMPQWRLELETMLKRGVKFEIEALSVHSLSFLSEVYIPSKI 355

BLAST of MC11g1041 vs. ExPASy Swiss-Prot
Match: Q5ZPV8 (Meiotic recombination protein SPO11-1 OS=Oryza sativa subsp. indica OX=39946 GN=SPO11-1 PE=2 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 1.1e-126
Identity = 213/359 (59.33%), Postives = 279/359 (77.72%), Query Frame = 0

Query: 2   EGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTN----SLENCCCKFN 61
           E +R   E + +L RIR + R ++ E++ G S  +A+ R++NYC++    +   C C ++
Sbjct: 17  ERRRRQEEAATLLHRIRGLVRWVVAEVAAGRSPTVALHRYQNYCSSASAAAASPCACSYD 76

Query: 62  LSEGKEILT-LQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVD 121
           +  G ++L+ L + SH  RL+VLLRVLL+V+QLL++N+H SKRDIYYM+PS+F +Q+VVD
Sbjct: 77  VPVGTDVLSLLHRGSHASRLNVLLRVLLVVQQLLQQNKHCSKRDIYYMYPSIFQEQAVVD 136

Query: 122 RAINDICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVT 181
           RAINDIC+L +CSRHNLNVV    GLVMGW+RF E  +   C+   N+A SIPV +E + 
Sbjct: 137 RAINDICVLFKCSRHNLNVVPVAKGLVMGWIRFLEGEKEVYCVTNVNAAFSIPVSIEAIK 196

Query: 182 DIVSAAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPA 241
           D+VS A+YILIVEKE+VFQRLAND+FC RNRCIVITGRGYPD+PTRRFLR LV+   LP 
Sbjct: 197 DVVSVADYILIVEKETVFQRLANDKFCERNRCIVITGRGYPDIPTRRFLRYLVEQLHLPV 256

Query: 242 FCLVDCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLT 301
           +CLVD DPYGF+IL TY+FGS+Q+AYDA +LR+P +RWLG F SD E+Y LP+ CL+ L+
Sbjct: 257 YCLVDADPYGFDILATYKFGSLQLAYDANFLRVPDIRWLGVFTSDFEDYRLPDCCLLHLS 316

Query: 302 MEDKRRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKI 356
            ED+R+ E +L RCYL RE P WRLEL++MLQKGVKFEIEALS  ++SFLSEEY+P KI
Sbjct: 317 SEDRRKAEGILSRCYLHREAPQWRLELEAMLQKGVKFEIEALSACSISFLSEEYIPKKI 375

BLAST of MC11g1041 vs. ExPASy Swiss-Prot
Match: Q7Y021 (Meiotic recombination protein SPO11-1 OS=Oryza sativa subsp. japonica OX=39947 GN=SPO11-1 PE=2 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 1.1e-126
Identity = 213/359 (59.33%), Postives = 279/359 (77.72%), Query Frame = 0

Query: 2   EGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTN----SLENCCCKFN 61
           E +R   E + +L RIR + R ++ E++ G S  +A+ R++NYC++    +   C C ++
Sbjct: 17  ERRRRQEEAATLLHRIRGLVRWVVAEVAAGRSPTVALHRYQNYCSSASAAAASPCACSYD 76

Query: 62  LSEGKEILT-LQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVD 121
           +  G ++L+ L + SH  RL+VLLRVLL+V+QLL++N+H SKRDIYYM+PS+F +Q+VVD
Sbjct: 77  VPVGTDVLSLLHRGSHASRLNVLLRVLLVVQQLLQQNKHCSKRDIYYMYPSIFQEQAVVD 136

Query: 122 RAINDICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVT 181
           RAINDIC+L +CSRHNLNVV    GLVMGW+RF E  +   C+   N+A SIPV +E + 
Sbjct: 137 RAINDICVLFKCSRHNLNVVPVAKGLVMGWIRFLEGEKEVYCVTNVNAAFSIPVSIEAIK 196

Query: 182 DIVSAAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPA 241
           D+VS A+YILIVEKE+VFQRLAND+FC RNRCIVITGRGYPD+PTRRFLR LV+   LP 
Sbjct: 197 DVVSVADYILIVEKETVFQRLANDKFCERNRCIVITGRGYPDIPTRRFLRYLVEQLHLPV 256

Query: 242 FCLVDCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLT 301
           +CLVD DPYGF+IL TY+FGS+Q+AYDA +LR+P +RWLG F SD E+Y LP+ CL+ L+
Sbjct: 257 YCLVDADPYGFDILATYKFGSLQLAYDANFLRVPDIRWLGVFTSDFEDYRLPDCCLLHLS 316

Query: 302 MEDKRRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKI 356
            ED+R+ E +L RCYL RE P WRLEL++MLQKGVKFEIEALS  ++SFLSEEY+P KI
Sbjct: 317 SEDRRKAEGILSRCYLHREAPQWRLELEAMLQKGVKFEIEALSACSISFLSEEYIPKKI 375

BLAST of MC11g1041 vs. ExPASy Swiss-Prot
Match: Q9Y5K1 (Meiotic recombination protein SPO11 OS=Homo sapiens OX=9606 GN=SPO11 PE=1 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.8e-60
Identity = 130/363 (35.81%), Postives = 225/363 (61.98%), Query Frame = 0

Query: 3   GKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSEGK 62
           G R +S  S++L  I  I + +I  L+   +    ID       +S EN   KF  S G 
Sbjct: 38  GSRLASS-SEVLASIENIIQDIITSLARNEAPAFTIDN-----RSSWEN--IKFEDSVGL 97

Query: 63  EILT------LQKES--HVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSV 122
           ++++      ++ +S    ++  ++L++L ++ +L++ N +++KRDIYY    +F +Q+V
Sbjct: 98  QMVSHCTTRKIKSDSPKSAQKFSLILKILSMIYKLVQSNTYATKRDIYYTDSQLFGNQTV 157

Query: 123 VDRAINDICILLQCSRHNLNVVSFGNGLVMGWLRF-SEAGRRFDCIKKPNSAHSIPVQVE 182
           VD  INDI  +L+ SR +L+++S   GL+ G LR+  E G + +C     +A ++P  ++
Sbjct: 158 VDNIINDISCMLKVSRRSLHILSTSKGLIAGNLRYIEEDGTKVNCTCGA-TAVAVPSNIQ 217

Query: 183 EVTDIVSAAEYILIVEKESVFQRLANDQFCSR-NRCIVITGRGYPDVPTRRFLRLLVDVS 242
            + ++V+ A+++LIVEK++ FQRL +D FC++ + CI+ITG+G PD+ TR  ++ L D  
Sbjct: 218 GIRNLVTDAKFVLIVEKDATFQRLLDDNFCNKLSPCIMITGKGVPDLNTRLLVKKLWDTF 277

Query: 243 ALPAFCLVDCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCL 302
            +P F LVD DP+G EI+  Y++GSM M+++A +L +P +RWLG   SD +  N+P+  L
Sbjct: 278 HVPVFTLVDADPHGIEIMCIYKYGSMSMSFEAHHLTVPAIRWLGLLPSDLKRLNVPKDSL 337

Query: 303 IPLTMEDKRRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLP 356
           IPLT  D+ + +++L R Y+  + P WR E++ M    +K EI+AL+  +  +LS  YLP
Sbjct: 338 IPLTKRDQMKLDSILRRPYVTCQ-PFWRKEMEIMADSKMKAEIQALTFLSSDYLSRVYLP 390

BLAST of MC11g1041 vs. ExPASy Swiss-Prot
Match: Q9WTK8 (Meiotic recombination protein SPO11 OS=Mus musculus OX=10090 GN=Spo11 PE=1 SV=2)

HSP 1 Score: 230.3 bits (586), Expect = 3.4e-59
Identity = 131/363 (36.09%), Postives = 217/363 (59.78%), Query Frame = 0

Query: 3   GKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSEGK 62
           G    +  S++L  I  I + +I  L+        ID       +S EN    F+ S G 
Sbjct: 37  GATRVASSSEVLTAIENIIQDIIKSLARNEVPAFTIDN-----RSSWEN--IMFDDSVGL 96

Query: 63  EIL---TLQK-----ESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSV 122
            ++   T +K        V++  ++L+VL ++ +L++ + +++KRDIYY    +F +Q+ 
Sbjct: 97  RMIPQCTTRKIRSDSPKSVKKFALILKVLSMIYKLIQSDTYATKRDIYYTDSQLFGNQAA 156

Query: 123 VDRAINDICILLQCSRHNLNVVSFGNGLVMGWLRF-SEAGRRFDCIKKPNSAHSIPVQVE 182
           VD AI+DI  +L+  R +L+V+S   GL+ G LR+  E G R  C     +A ++P  ++
Sbjct: 157 VDSAIDDISCMLKVPRRSLHVLSTSKGLIAGNLRYMEEDGTRVQCTCSA-TATAVPTNIQ 216

Query: 183 EVTDIVSAAEYILIVEKESVFQRLANDQFCSR-NRCIVITGRGYPDVPTRRFLRLLVDVS 242
            +  +++ A+++LIVEK++ FQRL +D FCSR + CI++TG+G PD+ TR  ++ L D  
Sbjct: 217 GMQHLITDAKFLLIVEKDATFQRLLDDNFCSRMSPCIMVTGKGVPDLNTRLLVKKLWDTF 276

Query: 243 ALPAFCLVDCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCL 302
            +P F LVD DPYG EI+  Y++GSM M+++A  L IP +RWLG   SD +  N+P+  L
Sbjct: 277 HIPVFTLVDADPYGIEIMCIYKYGSMSMSFEAHNLTIPTIRWLGLLPSDIQRLNIPKDSL 336

Query: 303 IPLTMEDKRRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLP 356
           IPLT  D+ + +++L R Y+  + P W+ EL+ M    +K EI+AL++ +  +LS  YLP
Sbjct: 337 IPLTKHDQMKLDSILKRPYITYQ-PLWKKELEMMADSKMKAEIQALTLLSSDYLSRVYLP 390

BLAST of MC11g1041 vs. NCBI nr
Match: XP_022139661.1 (meiotic recombination protein SPO11-1 [Momordica charantia])

HSP 1 Score: 731 bits (1887), Expect = 4.87e-266
Identity = 364/364 (100.00%), Postives = 364/364 (100.00%), Query Frame = 0

Query: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60
           MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE
Sbjct: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120
           GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN
Sbjct: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS 180
           DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS
Sbjct: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS 180

Query: 181 AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV 240
           AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV
Sbjct: 181 AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV 240

Query: 241 DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK 300
           DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK
Sbjct: 241 DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK 300

Query: 301 RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKIGCVHL 360
           RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKIGCVHL
Sbjct: 301 RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKIGCVHL 360

Query: 361 RTGK 364
           RTGK
Sbjct: 361 RTGK 364

BLAST of MC11g1041 vs. NCBI nr
Match: XP_023536168.1 (meiotic recombination protein SPO11-1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 651 bits (1679), Expect = 2.63e-234
Identity = 318/355 (89.58%), Postives = 339/355 (95.49%), Query Frame = 0

Query: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60
           M GK  SSER DI+R+IRE+TRSMIVEL N SSKM+AIDRFKNYCTNS+ENCCC+FNLSE
Sbjct: 1   MAGKLGSSERIDIMRKIRELTRSMIVELCNRSSKMIAIDRFKNYCTNSIENCCCRFNLSE 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120
           GKEILTLQ+ESHVRRLDVLLRVLLI++QLLRENRHSSKRDIYYMHPSVF+DQSVVDRAIN
Sbjct: 61  GKEILTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS 180
           DICILLQCSRHNLNVVS GNGLVMGWLRF+EAGRR DCIKKPNSAHSIPVQVEEVTDI S
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRSDCIKKPNSAHSIPVQVEEVTDIAS 180

Query: 181 AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV 240
            A YIL+VEKESVFQRLAND FCSRNRCIVI+GRGYPDVPTRRFLRLLVDV ALPAFCLV
Sbjct: 181 PANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLV 240

Query: 241 DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK 300
           DCDPYGF+ILTTYRFGSMQMAYDAKYLRIP L WLGAFASDS+ YNLP+QCLIPLT+EDK
Sbjct: 241 DCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLHWLGAFASDSDTYNLPQQCLIPLTLEDK 300

Query: 301 RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKI 355
           RRTEALLLRCYL+REVPHWRLEL+SMLQ+GVKFEIEALSVHN+SFLSEEYLPSKI
Sbjct: 301 RRTEALLLRCYLRREVPHWRLELESMLQRGVKFEIEALSVHNISFLSEEYLPSKI 355

BLAST of MC11g1041 vs. NCBI nr
Match: XP_022976331.1 (meiotic recombination protein SPO11-1 [Cucurbita maxima])

HSP 1 Score: 650 bits (1676), Expect = 6.50e-234
Identity = 317/355 (89.30%), Postives = 338/355 (95.21%), Query Frame = 0

Query: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60
           M G    SER DI+R+IRE+TRSMIVELSNGSSKM+AIDRFKNYC NS+ENCCC+FNLSE
Sbjct: 1   MAGNLGRSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCINSIENCCCRFNLSE 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120
           GKEILTLQ+ESHVRRLDVLLRVLLI++QLLRENRHSSKRDIYYMHPSVF+DQSVVDRAIN
Sbjct: 61  GKEILTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS 180
           DICILLQCSRHNLNVVS GNGLVMGWLRF+EAGRR DCIKKPNSAHSIPVQVEEVTDI S
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRSDCIKKPNSAHSIPVQVEEVTDIAS 180

Query: 181 AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV 240
            A YIL+VEKESVFQRLAND FCSRNRCIVI+GRGYPDVPTRRFLRLLVDV ALPAFCLV
Sbjct: 181 PANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLV 240

Query: 241 DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK 300
           DCDPYGF+ILTTYRFGSMQMAYDAKYLRIP L WLGAFASDS+ YNLP+QCLIPLT+EDK
Sbjct: 241 DCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLHWLGAFASDSDTYNLPQQCLIPLTLEDK 300

Query: 301 RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKI 355
           RRTEALLLRCYL+REVPHWRLEL+SMLQ+GVKFEIEALSVHN+SFLSEEYLPSKI
Sbjct: 301 RRTEALLLRCYLRREVPHWRLELESMLQRGVKFEIEALSVHNISFLSEEYLPSKI 355

BLAST of MC11g1041 vs. NCBI nr
Match: XP_022937001.1 (meiotic recombination protein SPO11-1 [Cucurbita moschata])

HSP 1 Score: 644 bits (1661), Expect = 1.57e-231
Identity = 316/357 (88.52%), Postives = 337/357 (94.40%), Query Frame = 0

Query: 1   MEGKRASSERS--DILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNL 60
           M GK  SSER   DI+R+IRE+TRSMIVELSNGSSKM+AIDRFKNYCTNS ENCCC+FNL
Sbjct: 1   MAGKLGSSERIRIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTENCCCRFNL 60

Query: 61  SEGKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRA 120
           SEGKEILTLQ++SHVRRLDVLLRVLLI++QLLRENRHSSKRDIYYMHPSVF+DQS VDRA
Sbjct: 61  SEGKEILTLQRQSHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSAVDRA 120

Query: 121 INDICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDI 180
           INDICILLQCSRHNLNVVS GNGLVMGWLRF+EAGRRFDCIKKPN+AHSIPVQVEEVTDI
Sbjct: 121 INDICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDI 180

Query: 181 VSAAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFC 240
              A YIL+VEKESVFQRLAND FCSRNRCIVI+GRGYPDVPTRRFLRLLVDV ALPAFC
Sbjct: 181 AGPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFC 240

Query: 241 LVDCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTME 300
           LVDCDPYGF+ILTTYRFGSMQMAYDAKYLRIP L WLGAFASDS  YNLP+QCLIPLT+E
Sbjct: 241 LVDCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLHWLGAFASDSNTYNLPQQCLIPLTLE 300

Query: 301 DKRRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKI 355
           DKRRTEALL RCYL+REVPHWRLEL+SMLQ+GVKFEIEALSVHN+SFLSEEYLPSKI
Sbjct: 301 DKRRTEALLPRCYLRREVPHWRLELESMLQRGVKFEIEALSVHNISFLSEEYLPSKI 357

BLAST of MC11g1041 vs. NCBI nr
Match: XP_008462989.1 (PREDICTED: meiotic recombination protein SPO11-1 isoform X1 [Cucumis melo])

HSP 1 Score: 642 bits (1657), Expect = 1.20e-230
Identity = 314/364 (86.26%), Postives = 343/364 (94.23%), Query Frame = 0

Query: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60
           MEG R S ER ++LR+I E+TRSMIVELSNGSSKM+AIDRFK+YCTNSLENCCC+FNLSE
Sbjct: 1   MEGNRGSLERIEVLRKITELTRSMIVELSNGSSKMIAIDRFKSYCTNSLENCCCRFNLSE 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120
           GK++LTLQ+ESHVRRLDVLLRVLLI++QLLRENRHSSKRD+YYMHPS+F+DQSVVDRAIN
Sbjct: 61  GKDVLTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDMYYMHPSIFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS 180
           DICILLQCSRHNLNVVS GNGLVMGWLRFSEAGRRFDC+KKPNSA  IPVQVEEVTDI +
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAQFIPVQVEEVTDIAT 180

Query: 181 AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV 240
           AA+YIL+VEKESVFQRLAND+FCSRNRCIVITGRGYPDVPTRRFLRLLVDV ALPAFCLV
Sbjct: 181 AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLV 240

Query: 241 DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK 300
           DCDPYGF+ILTTYRFGSMQMAYDAK LRIP L+W+GAFASDSE Y+LPEQCLIPLT +DK
Sbjct: 241 DCDPYGFDILTTYRFGSMQMAYDAKNLRIPQLQWIGAFASDSEKYDLPEQCLIPLTSKDK 300

Query: 301 RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKIGCVHL 360
           RRTEALL RCYL+REVPHWRLELDSMLQ+GVKFEIEALSVHN+SFLS+ YLPSKI    L
Sbjct: 301 RRTEALLQRCYLRREVPHWRLELDSMLQRGVKFEIEALSVHNISFLSDHYLPSKIES-EL 360

Query: 361 RTGK 364
            TGK
Sbjct: 361 TTGK 363

BLAST of MC11g1041 vs. ExPASy TrEMBL
Match: A0A6J1CDC7 (meiotic recombination protein SPO11-1 OS=Momordica charantia OX=3673 GN=LOC111010510 PE=3 SV=1)

HSP 1 Score: 731 bits (1887), Expect = 2.36e-266
Identity = 364/364 (100.00%), Postives = 364/364 (100.00%), Query Frame = 0

Query: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60
           MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE
Sbjct: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120
           GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN
Sbjct: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS 180
           DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS
Sbjct: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS 180

Query: 181 AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV 240
           AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV
Sbjct: 181 AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV 240

Query: 241 DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK 300
           DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK
Sbjct: 241 DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK 300

Query: 301 RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKIGCVHL 360
           RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKIGCVHL
Sbjct: 301 RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKIGCVHL 360

Query: 361 RTGK 364
           RTGK
Sbjct: 361 RTGK 364

BLAST of MC11g1041 vs. ExPASy TrEMBL
Match: A0A6J1IGM0 (meiotic recombination protein SPO11-1 OS=Cucurbita maxima OX=3661 GN=LOC111476764 PE=3 SV=1)

HSP 1 Score: 650 bits (1676), Expect = 3.15e-234
Identity = 317/355 (89.30%), Postives = 338/355 (95.21%), Query Frame = 0

Query: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60
           M G    SER DI+R+IRE+TRSMIVELSNGSSKM+AIDRFKNYC NS+ENCCC+FNLSE
Sbjct: 1   MAGNLGRSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCINSIENCCCRFNLSE 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120
           GKEILTLQ+ESHVRRLDVLLRVLLI++QLLRENRHSSKRDIYYMHPSVF+DQSVVDRAIN
Sbjct: 61  GKEILTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS 180
           DICILLQCSRHNLNVVS GNGLVMGWLRF+EAGRR DCIKKPNSAHSIPVQVEEVTDI S
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRSDCIKKPNSAHSIPVQVEEVTDIAS 180

Query: 181 AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV 240
            A YIL+VEKESVFQRLAND FCSRNRCIVI+GRGYPDVPTRRFLRLLVDV ALPAFCLV
Sbjct: 181 PANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLV 240

Query: 241 DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK 300
           DCDPYGF+ILTTYRFGSMQMAYDAKYLRIP L WLGAFASDS+ YNLP+QCLIPLT+EDK
Sbjct: 241 DCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLHWLGAFASDSDTYNLPQQCLIPLTLEDK 300

Query: 301 RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKI 355
           RRTEALLLRCYL+REVPHWRLEL+SMLQ+GVKFEIEALSVHN+SFLSEEYLPSKI
Sbjct: 301 RRTEALLLRCYLRREVPHWRLELESMLQRGVKFEIEALSVHNISFLSEEYLPSKI 355

BLAST of MC11g1041 vs. ExPASy TrEMBL
Match: A0A6J1F921 (meiotic recombination protein SPO11-1 OS=Cucurbita moschata OX=3662 GN=LOC111443423 PE=3 SV=1)

HSP 1 Score: 644 bits (1661), Expect = 7.59e-232
Identity = 316/357 (88.52%), Postives = 337/357 (94.40%), Query Frame = 0

Query: 1   MEGKRASSERS--DILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNL 60
           M GK  SSER   DI+R+IRE+TRSMIVELSNGSSKM+AIDRFKNYCTNS ENCCC+FNL
Sbjct: 1   MAGKLGSSERIRIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTENCCCRFNL 60

Query: 61  SEGKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRA 120
           SEGKEILTLQ++SHVRRLDVLLRVLLI++QLLRENRHSSKRDIYYMHPSVF+DQS VDRA
Sbjct: 61  SEGKEILTLQRQSHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSAVDRA 120

Query: 121 INDICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDI 180
           INDICILLQCSRHNLNVVS GNGLVMGWLRF+EAGRRFDCIKKPN+AHSIPVQVEEVTDI
Sbjct: 121 INDICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDI 180

Query: 181 VSAAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFC 240
              A YIL+VEKESVFQRLAND FCSRNRCIVI+GRGYPDVPTRRFLRLLVDV ALPAFC
Sbjct: 181 AGPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFC 240

Query: 241 LVDCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTME 300
           LVDCDPYGF+ILTTYRFGSMQMAYDAKYLRIP L WLGAFASDS  YNLP+QCLIPLT+E
Sbjct: 241 LVDCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLHWLGAFASDSNTYNLPQQCLIPLTLE 300

Query: 301 DKRRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKI 355
           DKRRTEALL RCYL+REVPHWRLEL+SMLQ+GVKFEIEALSVHN+SFLSEEYLPSKI
Sbjct: 301 DKRRTEALLPRCYLRREVPHWRLELESMLQRGVKFEIEALSVHNISFLSEEYLPSKI 357

BLAST of MC11g1041 vs. ExPASy TrEMBL
Match: A0A1S3CJR6 (meiotic recombination protein SPO11-1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501238 PE=3 SV=1)

HSP 1 Score: 642 bits (1657), Expect = 5.80e-231
Identity = 314/364 (86.26%), Postives = 343/364 (94.23%), Query Frame = 0

Query: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60
           MEG R S ER ++LR+I E+TRSMIVELSNGSSKM+AIDRFK+YCTNSLENCCC+FNLSE
Sbjct: 1   MEGNRGSLERIEVLRKITELTRSMIVELSNGSSKMIAIDRFKSYCTNSLENCCCRFNLSE 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120
           GK++LTLQ+ESHVRRLDVLLRVLLI++QLLRENRHSSKRD+YYMHPS+F+DQSVVDRAIN
Sbjct: 61  GKDVLTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDMYYMHPSIFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS 180
           DICILLQCSRHNLNVVS GNGLVMGWLRFSEAGRRFDC+KKPNSA  IPVQVEEVTDI +
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAQFIPVQVEEVTDIAT 180

Query: 181 AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV 240
           AA+YIL+VEKESVFQRLAND+FCSRNRCIVITGRGYPDVPTRRFLRLLVDV ALPAFCLV
Sbjct: 181 AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLV 240

Query: 241 DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK 300
           DCDPYGF+ILTTYRFGSMQMAYDAK LRIP L+W+GAFASDSE Y+LPEQCLIPLT +DK
Sbjct: 241 DCDPYGFDILTTYRFGSMQMAYDAKNLRIPQLQWIGAFASDSEKYDLPEQCLIPLTSKDK 300

Query: 301 RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKIGCVHL 360
           RRTEALL RCYL+REVPHWRLELDSMLQ+GVKFEIEALSVHN+SFLS+ YLPSKI    L
Sbjct: 301 RRTEALLQRCYLRREVPHWRLELDSMLQRGVKFEIEALSVHNISFLSDHYLPSKIES-EL 360

Query: 361 RTGK 364
            TGK
Sbjct: 361 TTGK 363

BLAST of MC11g1041 vs. ExPASy TrEMBL
Match: A0A1S3CI64 (meiotic recombination protein SPO11-1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103501238 PE=3 SV=1)

HSP 1 Score: 625 bits (1613), Expect = 2.61e-224
Identity = 311/364 (85.44%), Postives = 339/364 (93.13%), Query Frame = 0

Query: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60
           MEG R S ER ++LR+I E+TRSMIVELSNGSSKM+AIDRFK+YCTNSLEN    FNLSE
Sbjct: 1   MEGNRGSLERIEVLRKITELTRSMIVELSNGSSKMIAIDRFKSYCTNSLENW---FNLSE 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120
           GK++LTLQ+ESHVRRLDVLLRVLLI++QLLRENRHSSKRD+YYMHPS+F+DQSVVDRAIN
Sbjct: 61  GKDVLTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDMYYMHPSIFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS 180
           DICILLQCSRHNLNVVS GNGLVMGWLRFSEAGRRFDC+KKPNSA  IPVQVEEVTDI +
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAQFIPVQVEEVTDIAT 180

Query: 181 AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV 240
           AA+YIL+VEKESVFQRLAND+FCSRNRCIVITGRGYPDVPTRRFLRLLVDV ALPAFCLV
Sbjct: 181 AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLV 240

Query: 241 DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK 300
           DCDPYGF+ILTTYRFGSMQMAYDAK LRIP L+W+GAFASDSE Y+LPEQCLIPLT +DK
Sbjct: 241 DCDPYGFDILTTYRFGSMQMAYDAKNLRIPQLQWIGAFASDSEKYDLPEQCLIPLTSKDK 300

Query: 301 RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKIGCVHL 360
           RRTEALL RCYL+REVPHWRLELDSMLQ+GVKFEIEALSVHN+SFLS+ YLPSKI    L
Sbjct: 301 RRTEALLQRCYLRREVPHWRLELDSMLQRGVKFEIEALSVHNISFLSDHYLPSKIES-EL 360

Query: 361 RTGK 364
            TGK
Sbjct: 361 TTGK 360

BLAST of MC11g1041 vs. TAIR 10
Match: AT3G13170.1 (Spo11/DNA topoisomerase VI, subunit A protein )

HSP 1 Score: 513.1 bits (1320), Expect = 1.9e-145
Identity = 245/355 (69.01%), Postives = 300/355 (84.51%), Query Frame = 0

Query: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60
           MEGK A SE +++L+RI++ T+S++V+L+ G S  ++I++F+NYC N   +C C  +  +
Sbjct: 1   MEGKFAISESTNLLQRIKDFTQSVVVDLAEGRSPKISINQFRNYCMNPEADCLCSSDKPK 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120
           G+EI TL+KE    R+D+LLRVLLIV+QLL+ENRH+SKRDIYYMHPS F  QS+VDRAI 
Sbjct: 61  GQEIFTLKKEPQTYRIDMLLRVLLIVQQLLQENRHASKRDIYYMHPSAFKAQSIVDRAIG 120

Query: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS 180
           DICIL QCSR+NLNVVS GNGLVMGWL+F EAGR+FDC+   N+A+ +PV VEEV DIVS
Sbjct: 121 DICILFQCSRYNLNVVSVGNGLVMGWLKFREAGRKFDCLNSLNTAYPVPVLVEEVEDIVS 180

Query: 181 AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV 240
            AEYIL+VEKE+VFQRLAND FC  NRCIVITGRGYPDV TRRFLRLL++   LP  CLV
Sbjct: 181 LAEYILVVEKETVFQRLANDMFCKTNRCIVITGRGYPDVSTRRFLRLLMEKLHLPVHCLV 240

Query: 241 DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK 300
           DCDPYGFEIL TYRFGSMQMAYD + LR P ++WLGAF SDSE Y++P+QCL+PLT EDK
Sbjct: 241 DCDPYGFEILATYRFGSMQMAYDIESLRAPDMKWLGAFPSDSEVYSVPKQCLLPLTEEDK 300

Query: 301 RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKI 356
           +RTEA+LLRCYL+RE+P WRLEL++ML++GVKFEIEALSVH+LSFLSE Y+PSKI
Sbjct: 301 KRTEAMLLRCYLKREMPQWRLELETMLKRGVKFEIEALSVHSLSFLSEVYIPSKI 355

BLAST of MC11g1041 vs. TAIR 10
Match: AT5G02820.1 (Spo11/DNA topoisomerase VI, subunit A protein )

HSP 1 Score: 226.5 bits (576), Expect = 3.5e-59
Identity = 114/285 (40.00%), Postives = 174/285 (61.05%), Query Frame = 0

Query: 71  SHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAINDICILLQCSR 130
           S VR+  +  R+L ++ QL   N H +KRD++Y    +F DQ+  D  ++D+  +L C+R
Sbjct: 138 SSVRKTTITTRILALIHQLCLRNIHVTKRDLFYTDVKLFQDQTQSDAVLDDVSCMLGCTR 197

Query: 131 HNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVSAAEYILIVEK 190
            +LNV++   G+V+G L FS+ G   DC K      +IP  ++ V D+ S A +IL+VEK
Sbjct: 198 SSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQSDAMFILLVEK 257

Query: 191 ESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLVDCDPYGFEIL 250
           ++ + RLA D+F +R  CI++T +G PDV TR FLR +     LP   LVD DPYG +IL
Sbjct: 258 DAAYMRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALVDSDPYGLKIL 317

Query: 251 TTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDKRRTEALLLRC 310
           + Y  GS  M+YD+  L  P ++WLG   SD + Y +PEQC +P+T +D +  + +L   
Sbjct: 318 SVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYKIPEQCRLPMTEQDIKTGKDMLEED 377

Query: 311 YLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKI 356
           ++++  P W  EL+ M++   K EI+ALS     +LSE YLP K+
Sbjct: 378 FVKKN-PGWVEELNLMVKTKQKAEIQALSSFGFQYLSEVYLPLKL 421

BLAST of MC11g1041 vs. TAIR 10
Match: AT1G63990.1 (sporulation 11-2 )

HSP 1 Score: 152.9 bits (385), Expect = 4.9e-37
Identity = 98/283 (34.63%), Postives = 160/283 (56.54%), Query Frame = 0

Query: 81  RVLLIVRQLLRENRHSSKRDIYYM----HPSVFSDQSVVDRAINDICILLQCSRHNLNVV 140
           +V+ I  Q+L + +  ++R+++Y      P  FS Q  V+R++ D+  LL+CSR++L ++
Sbjct: 102 KVMEICFQILLQEKRVTQRELFYKLLCDSPDYFSSQIEVNRSVQDVVALLRCSRYSLGIM 161

Query: 141 SFGNGLVMGWLRFSEAGRR-FDCIKKPNSAHSI--PVQVEEVTDIVSAAEYILIVEKESV 200
           +   GLV G L   E G+   DC    +S  +I   + + + T + + A YI+IVEK ++
Sbjct: 162 ASSRGLVAGRLFLQEPGKEAVDCSACGSSGFAITGDLNLLDNTIMRTDARYIIIVEKHAI 221

Query: 201 FQRLANDQFCSRNRCIVITGRGYPDVPTRRFL-RLLVDVSALPAFCLVDCDPYGFEILTT 260
           F RL  D+  +   C+ IT +GYPD+ TR FL R+      LP   LVD +P G  IL T
Sbjct: 222 FHRLVEDRVFNHIPCVFITAKGYPDIATRFFLHRMSTTFPDLPILVLVDWNPAGLAILCT 281

Query: 261 YRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDKRRTEALLLRCYL 320
           ++FGS+ M  +A Y    +++W+G    D     +PE+ L+PL  +D +  ++LL    L
Sbjct: 282 FKFGSIGMGLEA-YRYACNVKWIGLRGDDLN--LIPEESLVPLKPKDSQIAKSLLSSKIL 341

Query: 321 QREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKI 356
           Q    ++  EL  M+Q G + EIEAL  H  ++L  +Y+ +KI
Sbjct: 342 QE---NYIEELSLMVQTGKRAEIEALYCHGYNYLG-KYIATKI 377

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M4A22.6e-14469.01Meiotic recombination protein SPO11-1 OS=Arabidopsis thaliana OX=3702 GN=SPO11-1... [more]
Q5ZPV81.1e-12659.33Meiotic recombination protein SPO11-1 OS=Oryza sativa subsp. indica OX=39946 GN=... [more]
Q7Y0211.1e-12659.33Meiotic recombination protein SPO11-1 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q9Y5K11.8e-6035.81Meiotic recombination protein SPO11 OS=Homo sapiens OX=9606 GN=SPO11 PE=1 SV=1[more]
Q9WTK83.4e-5936.09Meiotic recombination protein SPO11 OS=Mus musculus OX=10090 GN=Spo11 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_022139661.14.87e-266100.00meiotic recombination protein SPO11-1 [Momordica charantia][more]
XP_023536168.12.63e-23489.58meiotic recombination protein SPO11-1 [Cucurbita pepo subsp. pepo][more]
XP_022976331.16.50e-23489.30meiotic recombination protein SPO11-1 [Cucurbita maxima][more]
XP_022937001.11.57e-23188.52meiotic recombination protein SPO11-1 [Cucurbita moschata][more]
XP_008462989.11.20e-23086.26PREDICTED: meiotic recombination protein SPO11-1 isoform X1 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1CDC72.36e-266100.00meiotic recombination protein SPO11-1 OS=Momordica charantia OX=3673 GN=LOC11101... [more]
A0A6J1IGM03.15e-23489.30meiotic recombination protein SPO11-1 OS=Cucurbita maxima OX=3661 GN=LOC11147676... [more]
A0A6J1F9217.59e-23288.52meiotic recombination protein SPO11-1 OS=Cucurbita moschata OX=3662 GN=LOC111443... [more]
A0A1S3CJR65.80e-23186.26meiotic recombination protein SPO11-1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A1S3CI642.61e-22485.44meiotic recombination protein SPO11-1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT3G13170.11.9e-14569.01Spo11/DNA topoisomerase VI, subunit A protein [more]
AT5G02820.13.5e-5940.00Spo11/DNA topoisomerase VI, subunit A protein [more]
AT1G63990.14.9e-3734.63sporulation 11-2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013048Meiotic recombination, Spo11PRINTSPR01551SPO11HOMOLOGcoord: 246..265
score: 45.0
coord: 342..361
score: 30.0
coord: 104..124
score: 40.14
coord: 199..214
score: 27.68
coord: 281..305
score: 36.0
IPR002815Spo11/DNA topoisomerase VI subunit APRINTSPR01550TOP6AFAMILYcoord: 112..129
score: 43.06
coord: 130..145
score: 41.02
coord: 241..257
score: 55.88
coord: 209..230
score: 46.88
coord: 98..109
score: 50.0
coord: 185..202
score: 54.51
IPR002815Spo11/DNA topoisomerase VI subunit APANTHERPTHR10848MEIOTIC RECOMBINATION PROTEIN SPO11coord: 4..356
NoneNo IPR availableGENE3D3.40.1360.10coord: 140..358
e-value: 1.8E-80
score: 271.7
NoneNo IPR availablePANTHERPTHR10848:SF3MEIOTIC RECOMBINATION PROTEIN SPO11-1coord: 4..356
IPR013049Spo11/DNA topoisomerase VI, subunit A, N-terminalPFAMPF04406TP6A_Ncoord: 74..135
e-value: 5.5E-21
score: 74.3
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 1..137
e-value: 3.0E-23
score: 84.2
IPR034136Topoisomerase 6 subunit A/Spo11, TOPRIM domainCDDcd00223TOPRIM_TopoIIB_SPOcoord: 183..345
e-value: 3.76344E-66
score: 204.796
IPR036078Spo11/DNA topoisomerase VI subunit A superfamilySUPERFAMILY56726DNA topoisomerase IV, alpha subunitcoord: 73..355

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC11g1041.1MC11g1041.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051026 chiasma assembly
biological_process GO:0000724 double-strand break repair via homologous recombination
biological_process GO:0042138 meiotic DNA double-strand break formation
biological_process GO:0006259 DNA metabolic process
cellular_component GO:0005694 chromosome
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003824 catalytic activity