MC11g0707 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC11g0707
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionchaperone protein DnaJ
LocationMC11: 5694338 .. 5697131 (-)
RNA-Seq ExpressionMC11g0707
SyntenyMC11g0707
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGTACATAGTCTAGAGACGAAACGAGATTTAATTAAGCTTAAAAGAAAGAAATGGATTGGTTGGAGATAATGGATAAATACAATCAAATTAACATCAAATAAAGAAAACAAATTTTTTTTTTTTTTTTCATTTGCCTCTTAGGACGGTGCCACGTCATCATCTTCTTGTTAACTGAATACCCAAATAAAAATGCAGTTTCAGAAAAGCTTGGTTATTTTGTTAACCTTCACAATTTTTGTGGACGCAGTAAAAAAAAACATCTATTTTCTTTTTTCTCTCTCTTTCTCTCTCTCTCTCTCCTCCTCCCTCTCTCATGGTGGATCTTCCCCAGGCTATTGGCATCTCCTTTCGAAACTTCTGCAAATCCTACAAATCTTTCTTCAAAAAATGGCATTCTAAGATCAAATCTTCGAAAAAAGATTCCGATGTTGATCAGAAGCGCGAAGGTTCGTCCGACGCCATTGATGGACCTTCAAAGGTACGCCACCTCCATTCTCTCAATTCTTTCCGCGGAATATCACGCCTCTTGTTCAGTTCAATCAGGATTACAAGTTTTTGAGGATTTCGAATTCATATATTCGTCGTTCTTTCTTTTCATACCGATTTCCGGATCCTTGATCTGCGGATGATTTTGATCGATCGAACAAGCCCTAATTTGAAGAAATTGACCAAAAAAATTCACTCGATCGCGTAACGGATTTTTTTTCAGGATCGTGAAGACGTTAAACGCGAAGGACGAGCGACGGCGACGGCGACGGCGACGAAAGGCGTTCCGAGCTTCAGATATGGAGGGAGCATGAGAGAGAATCATGATCCGCTGAGTTTCAGGCCTCGAAGCTACGACAGTGGATTCTCTCTGTCCCGGAATGCGAGTCGGAAAAGTCAAAATACGCATTCGTCGTCGCCTCTGTTTCGGAGCGTTAGCCGGAGAAGCACGGAATCGTCGGTGCCGTCTCGGGTCAGCAGCGGGCGGAGGAGCGTCGACTTGGTGTCGTCTCCTCCGTTACTGTCGAAAAGCGGTAGCCGGAGGAGCACGACGCCGATCATGTTCTCAAATTCCTCCGGCGTGCTGAAGCCGGCGCCAATCGAGAAGCAGCTCGAATGCACGCTGGAGGAGCTCTGCTTCGGATGCACCAAGAAAATCAAAATCACCAGAGACGTCCTCTCCAACACCACAGGGTTCGTTCCTCTCCTTACTAAACCCTAACGCCTATTTCCCTTTCAATTTCATATATAAGATTTTCCTTTTTCTTTTTACATTTTCACTTTTCTATATAATACAAATACATTCTTTCCTAAATTACAAAAATACCTCTCAAACTTCGATATACACACACTACACAACTAAGGTTTGGTCTCAATTTGATACATTGATATTTCTCTAAAAGTTAAATTAAAAAATCATAAAATATTTAAATTAACAAATAAATATTTTCATATTTTTAAATAAGTTAAGAAATAATTAATATTGATATTAGGTATATATGGATGATATTGTTCATATTTTGTCAATTTTTTGTACTATAATGGAAATGTTGATTTACCTCTCATTCGATTGGCAAACCGCGACGGATATTTAAAACCATGCACACAACGGATATTTAGAACCACCATGCACCCAAGACAGCTACAAAATTTTATATATTTCTTTTTCTTTTTACATTTTCACTTCTTTTATATATCTATATATATATAATCTTTAGTTAAATTACAGAAATATACTTCAATTTTACTCTTGTTTCAAAAATACCCTCATCTTTTCAAAGATTATCCTAAACATCAGGTAGTTGAGATAGAATACGATGTGGCAGTTATATTTCTTATTTGTCCTCTTCGTTATTATCTACCTAATTTTGTTTTATTCCCTCTTTTTAAGTCTCTACCTCTCTTTTCAGGCAGTGGTTTCGACGTTTTGGATGTTTGATGAATGATATTTTTGGAAATTGAAATATACATATTATAAGAAATAACTTTATAAAATATAACATTATACAACCTCTAGGGATGGCAATTGAATTTGGAAAAGATAAAATAAAATAAAATCTGAGAGATTGTATTGGGTGGCGATTGACAGGCAAACGACGGAGGAAGAGGAAACGCTGACGATAAACGTGAAGCCGGGGTGGAAAAAGGGGACGAAGATAACATTCGAGGGGATGGGGAACGAGAGGCCAGGGAGTTATCCAGCCGACATGTCGTTCGTGATTGCTGAGAAACGACACGCGTTTTTCAAATGGGAAGGGGACGACTTGGAGATGACGGTGGAGATTCCGCTGCTGAAAGCTCTAACTGGGTGCACAATTTCGGTGCCGCTGTTGGGAGGAGAAGCAATGAGCTTGGAGATAGACGACGTCGTATCGCCTGGCTACGAGAGGATCATCCAAGGCCAAGGTATGCCAAATCCTAAAGACGATGACAAGAGAGGCGACTTGAAAGTGAAGTTTCTGGTTGAATTTCCAACCCAATTAACTGACCAACAACGATCTGATGTTTGTCGAATTTTAGAGGATGGTTGTGTCTAATTTCTAGTTAACGAATTTTATTTAGGAATTAACTCAATTCTTCTTTTAATTTGTTTGTTTGTTTTGCTTGTAATGCATGTCTTTTGGTTTTTGAGCGAAAAGTGTGCAGTCTTTGCATTTGATTTGATTTATTTGAGGTAGAACAAGCTTTAATCTATCTTTTTTTTTTTTCTTTTTCATAGATAGAGCGTCGGCTTTTGTTTGGGACGCTCGTTTCTGACCCTAATCCTAACTCTCTTTGGTTTCGTATGACTTTTAACCCTCTTTTAT

mRNA sequence

GTGTACATAGTCTAGAGACGAAACGAGATTTAATTAAGCTTAAAAGAAAGAAATGGATTGGTTGGAGATAATGGATAAATACAATCAAATTAACATCAAATAAAGAAAACAAATTTTTTTTTTTTTTTTCATTTGCCTCTTAGGACGGTGCCACGTCATCATCTTCTTGTTAACTGAATACCCAAATAAAAATGCAGTTTCAGAAAAGCTTGGTTATTTTGTTAACCTTCACAATTTTTGTGGACGCAGTAAAAAAAAACATCTATTTTCTTTTTTCTCTCTCTTTCTCTCTCTCTCTCTCCTCCTCCCTCTCTCATGGTGGATCTTCCCCAGGCTATTGGCATCTCCTTTCGAAACTTCTGCAAATCCTACAAATCTTTCTTCAAAAAATGGCATTCTAAGATCAAATCTTCGAAAAAAGATTCCGATGTTGATCAGAAGCGCGAAGGTTCGTCCGACGCCATTGATGGACCTTCAAAGGATCGTGAAGACGTTAAACGCGAAGGACGAGCGACGGCGACGGCGACGGCGACGAAAGGCGTTCCGAGCTTCAGATATGGAGGGAGCATGAGAGAGAATCATGATCCGCTGAGTTTCAGGCCTCGAAGCTACGACAGTGGATTCTCTCTGTCCCGGAATGCGAGTCGGAAAAGTCAAAATACGCATTCGTCGTCGCCTCTGTTTCGGAGCGTTAGCCGGAGAAGCACGGAATCGTCGGTGCCGTCTCGGGTCAGCAGCGGGCGGAGGAGCGTCGACTTGGTGTCGTCTCCTCCGTTACTGTCGAAAAGCGGTAGCCGGAGGAGCACGACGCCGATCATGTTCTCAAATTCCTCCGGCGTGCTGAAGCCGGCGCCAATCGAGAAGCAGCTCGAATGCACGCTGGAGGAGCTCTGCTTCGGATGCACCAAGAAAATCAAAATCACCAGAGACGTCCTCTCCAACACCACAGGGCAAACGACGGAGGAAGAGGAAACGCTGACGATAAACGTGAAGCCGGGGTGGAAAAAGGGGACGAAGATAACATTCGAGGGGATGGGGAACGAGAGGCCAGGGAGTTATCCAGCCGACATGTCGTTCGTGATTGCTGAGAAACGACACGCGTTTTTCAAATGGGAAGGGGACGACTTGGAGATGACGGTGGAGATTCCGCTGCTGAAAGCTCTAACTGGGTGCACAATTTCGGTGCCGCTGTTGGGAGGAGAAGCAATGAGCTTGGAGATAGACGACGTCGTATCGCCTGGCTACGAGAGGATCATCCAAGGCCAAGGTATGCCAAATCCTAAAGACGATGACAAGAGAGGCGACTTGAAAGTGAAGTTTCTGGTTGAATTTCCAACCCAATTAACTGACCAACAACGATCTGATGTTTGTCGAATTTTAGAGGATGGTTGTGTCTAATTTCTAGTTAACGAATTTTATTTAGGAATTAACTCAATTCTTCTTTTAATTTGTTTGTTTGTTTTGCTTGTAATGCATGTCTTTTGGTTTTTGAGCGAAAAGTGTGCAGTCTTTGCATTTGATTTGATTTATTTGAGGTAGAACAAGCTTTAATCTATCTTTTTTTTTTTTCTTTTTCATAGATAGAGCGTCGGCTTTTGTTTGGGACGCTCGTTTCTGACCCTAATCCTAACTCTCTTTGGTTTCGTATGACTTTTAACCCTCTTTTAT

Coding sequence (CDS)

ATGGTGGATCTTCCCCAGGCTATTGGCATCTCCTTTCGAAACTTCTGCAAATCCTACAAATCTTTCTTCAAAAAATGGCATTCTAAGATCAAATCTTCGAAAAAAGATTCCGATGTTGATCAGAAGCGCGAAGGTTCGTCCGACGCCATTGATGGACCTTCAAAGGATCGTGAAGACGTTAAACGCGAAGGACGAGCGACGGCGACGGCGACGGCGACGAAAGGCGTTCCGAGCTTCAGATATGGAGGGAGCATGAGAGAGAATCATGATCCGCTGAGTTTCAGGCCTCGAAGCTACGACAGTGGATTCTCTCTGTCCCGGAATGCGAGTCGGAAAAGTCAAAATACGCATTCGTCGTCGCCTCTGTTTCGGAGCGTTAGCCGGAGAAGCACGGAATCGTCGGTGCCGTCTCGGGTCAGCAGCGGGCGGAGGAGCGTCGACTTGGTGTCGTCTCCTCCGTTACTGTCGAAAAGCGGTAGCCGGAGGAGCACGACGCCGATCATGTTCTCAAATTCCTCCGGCGTGCTGAAGCCGGCGCCAATCGAGAAGCAGCTCGAATGCACGCTGGAGGAGCTCTGCTTCGGATGCACCAAGAAAATCAAAATCACCAGAGACGTCCTCTCCAACACCACAGGGCAAACGACGGAGGAAGAGGAAACGCTGACGATAAACGTGAAGCCGGGGTGGAAAAAGGGGACGAAGATAACATTCGAGGGGATGGGGAACGAGAGGCCAGGGAGTTATCCAGCCGACATGTCGTTCGTGATTGCTGAGAAACGACACGCGTTTTTCAAATGGGAAGGGGACGACTTGGAGATGACGGTGGAGATTCCGCTGCTGAAAGCTCTAACTGGGTGCACAATTTCGGTGCCGCTGTTGGGAGGAGAAGCAATGAGCTTGGAGATAGACGACGTCGTATCGCCTGGCTACGAGAGGATCATCCAAGGCCAAGGTATGCCAAATCCTAAAGACGATGACAAGAGAGGCGACTTGAAAGTGAAGTTTCTGGTTGAATTTCCAACCCAATTAACTGACCAACAACGATCTGATGTTTGTCGAATTTTAGAGGATGGTTGTGTCTAA

Protein sequence

MVDLPQAIGISFRNFCKSYKSFFKKWHSKIKSSKKDSDVDQKREGSSDAIDGPSKDREDVKREGRATATATATKGVPSFRYGGSMRENHDPLSFRPRSYDSGFSLSRNASRKSQNTHSSSPLFRSVSRRSTESSVPSRVSSGRRSVDLVSSPPLLSKSGSRRSTTPIMFSNSSGVLKPAPIEKQLECTLEELCFGCTKKIKITRDVLSNTTGQTTEEEETLTINVKPGWKKGTKITFEGMGNERPGSYPADMSFVIAEKRHAFFKWEGDDLEMTVEIPLLKALTGCTISVPLLGGEAMSLEIDDVVSPGYERIIQGQGMPNPKDDDKRGDLKVKFLVEFPTQLTDQQRSDVCRILEDGCV
Homology
BLAST of MC11g0707 vs. ExPASy Swiss-Prot
Match: Q80Y75 (DnaJ homolog subfamily B member 13 OS=Mus musculus OX=10090 GN=Dnajb13 PE=1 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 2.3e-39
Identity = 79/169 (46.75%), Postives = 118/169 (69.82%), Query Frame = 0

Query: 180 PIEKQLECTLEELCFGCTKKIKITRDVLSNTTGQTTEEEETLTINVKPGWKKGTKITFEG 239
           PIE+ L  +LE+L FGCTKKIKI+R VL+     +T +++ LTI+V+PGW++GT+ITFE 
Sbjct: 139 PIERDLYLSLEDLFFGCTKKIKISRRVLNEDRYSSTIKDKILTIDVRPGWRQGTRITFEK 198

Query: 240 MGNERPGSYPADMSFVIAEKRHAFFKWEGDDLEMTVEIPLLKALTGCTISVPLLGGEAMS 299
            G++ P   PAD+ F++ EK H  F+ E D+L     IPL KALT CT+ V  L    ++
Sbjct: 199 EGDQGPNIIPADIIFIVKEKLHPRFRREHDNLFFVYPIPLGKALTCCTVEVKTLDDRLLN 258

Query: 300 LEIDDVVSPGYERIIQGQGMPNPKDDDKRGDLKVKFLVEFPTQLTDQQR 349
           + I+D+V P Y +I+ G+GMP P++  K+GDL + F ++FPT+LT Q++
Sbjct: 259 IPINDIVHPKYFKIVPGEGMPLPENPSKKGDLFIFFDIQFPTRLTPQKK 307

BLAST of MC11g0707 vs. ExPASy Swiss-Prot
Match: P59910 (DnaJ homolog subfamily B member 13 OS=Homo sapiens OX=9606 GN=DNAJB13 PE=1 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 8.6e-39
Identity = 81/176 (46.02%), Postives = 120/176 (68.18%), Query Frame = 0

Query: 174 GVLKPAP-IEKQLECTLEELCFGCTKKIKITRDVLSNTTGQTTEEEETLTINVKPGWKKG 233
           GV K  P +E+ L  +LE+L FGCTKKIKI+R VL+     +T +++ LTI+VKPGW++G
Sbjct: 132 GVKKQDPQVERDLYLSLEDLFFGCTKKIKISRRVLNEDGYSSTIKDKILTIDVKPGWRQG 191

Query: 234 TKITFEGMGNERPGSYPADMSFVIAEKRHAFFKWEGDDLEMTVEIPLLKALTGCTISVPL 293
           T+ITFE  G++ P   PAD+ F++ EK H  F+ E D+L     IPL KALT CT+ V  
Sbjct: 192 TRITFEKEGDQGPNIIPADIIFIVKEKLHPRFRRENDNLFFVNPIPLGKALTCCTVEVRT 251

Query: 294 LGGEAMSLEIDDVVSPGYERIIQGQGMPNPKDDDKRGDLKVKFLVEFPTQLTDQQR 349
           L    +++ I+D++ P Y + + G+GMP P+D  K+GDL + F ++FPT+LT Q++
Sbjct: 252 LDDRLLNIPINDIIHPKYFKKVPGEGMPLPEDPTKKGDLFIFFDIQFPTRLTPQKK 307

BLAST of MC11g0707 vs. ExPASy Swiss-Prot
Match: Q9UDY4 (DnaJ homolog subfamily B member 4 OS=Homo sapiens OX=9606 GN=DNAJB4 PE=1 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.5e-35
Identity = 73/169 (43.20%), Postives = 109/169 (64.50%), Query Frame = 0

Query: 180 PIEKQLECTLEELCFGCTKKIKITRDVLSNTTGQTTEEEETLTINVKPGWKKGTKITFEG 239
           P+  +L  +LEE+  GCTK++KI+R  L+        E++ LTI +K GWK+GTKITF  
Sbjct: 159 PVIHELRVSLEEIYSGCTKRMKISRKRLNADGRSYRSEDKILTIEIKKGWKEGTKITFPR 218

Query: 240 MGNERPGSYPADMSFVIAEKRHAFFKWEGDDLEMTVEIPLLKALTGCTISVPLLGGEAMS 299
            G+E P S PAD+ F+I +K H  FK +G ++  T +I L +AL GC+I+VP L G  + 
Sbjct: 219 EGDETPNSIPADIVFIIKDKDHPKFKRDGSNIIYTAKISLREALCGCSINVPTLDGRNIP 278

Query: 300 LEIDDVVSPGYERIIQGQGMPNPKDDDKRGDLKVKFLVEFPTQLTDQQR 349
           + ++D+V PG  R I G G+P PK+ D+RGDL ++F V FP  ++   +
Sbjct: 279 MSVNDIVKPGMRRRIIGYGLPFPKNPDQRGDLLIEFEVSFPDTISSSSK 327

BLAST of MC11g0707 vs. ExPASy Swiss-Prot
Match: Q5R8J8 (DnaJ homolog subfamily B member 4 OS=Pongo abelii OX=9601 GN=DNAJB4 PE=2 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.5e-35
Identity = 73/169 (43.20%), Postives = 109/169 (64.50%), Query Frame = 0

Query: 180 PIEKQLECTLEELCFGCTKKIKITRDVLSNTTGQTTEEEETLTINVKPGWKKGTKITFEG 239
           P+  +L  +LEE+  GCTK++KI+R  L+        E++ LTI +K GWK+GTKITF  
Sbjct: 159 PVIHELRVSLEEIYSGCTKRMKISRKRLNADGRSYRSEDKILTIEIKKGWKEGTKITFPR 218

Query: 240 MGNERPGSYPADMSFVIAEKRHAFFKWEGDDLEMTVEIPLLKALTGCTISVPLLGGEAMS 299
            G+E P S PAD+ F+I +K H  FK +G ++  T +I L +AL GC+I+VP L G  + 
Sbjct: 219 EGDETPNSIPADIVFIIKDKDHPKFKRDGSNIIYTAKISLREALCGCSINVPTLDGRNIP 278

Query: 300 LEIDDVVSPGYERIIQGQGMPNPKDDDKRGDLKVKFLVEFPTQLTDQQR 349
           + ++D+V PG  R I G G+P PK+ D+RGDL ++F V FP  ++   +
Sbjct: 279 MSVNDIVKPGMRRRIIGYGLPFPKNPDQRGDLLIEFEVSFPDTISSSSK 327

BLAST of MC11g0707 vs. ExPASy Swiss-Prot
Match: Q2KIT4 (DnaJ homolog subfamily B member 4 OS=Bos taurus OX=9913 GN=DNAJB4 PE=2 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 2.6e-35
Identity = 74/169 (43.79%), Postives = 109/169 (64.50%), Query Frame = 0

Query: 180 PIEKQLECTLEELCFGCTKKIKITRDVLSNTTGQTTEEEETLTINVKPGWKKGTKITFEG 239
           P+  +L  +LEE+  GCTK++KI+R  L+        E++ LTI +K GWK+GTKITF  
Sbjct: 159 PVIHELRVSLEEIYSGCTKRMKISRKRLNPDGRSYRTEDKILTIEIKKGWKEGTKITFPR 218

Query: 240 MGNERPGSYPADMSFVIAEKRHAFFKWEGDDLEMTVEIPLLKALTGCTISVPLLGGEAMS 299
            G+E P S PAD+ FVI +K H  FK +G ++  T +I L +AL GC+I+VP + G  + 
Sbjct: 219 EGDETPTSIPADIVFVIKDKDHPKFKRDGSNIIYTAKISLREALCGCSINVPTMDGRTIP 278

Query: 300 LEIDDVVSPGYERIIQGQGMPNPKDDDKRGDLKVKFLVEFPTQLTDQQR 349
           + I+D+V PG  R I G G+P PK+ D+RGDL ++F V FP  ++   +
Sbjct: 279 MTINDIVKPGMRRRIIGYGLPFPKNPDQRGDLLIEFEVSFPDTISSSSK 327

BLAST of MC11g0707 vs. NCBI nr
Match: XP_022132204.1 (uncharacterized protein LOC111005118 [Momordica charantia])

HSP 1 Score: 704 bits (1817), Expect = 1.61e-255
Identity = 360/360 (100.00%), Postives = 360/360 (100.00%), Query Frame = 0

Query: 1   MVDLPQAIGISFRNFCKSYKSFFKKWHSKIKSSKKDSDVDQKREGSSDAIDGPSKDREDV 60
           MVDLPQAIGISFRNFCKSYKSFFKKWHSKIKSSKKDSDVDQKREGSSDAIDGPSKDREDV
Sbjct: 1   MVDLPQAIGISFRNFCKSYKSFFKKWHSKIKSSKKDSDVDQKREGSSDAIDGPSKDREDV 60

Query: 61  KREGRATATATATKGVPSFRYGGSMRENHDPLSFRPRSYDSGFSLSRNASRKSQNTHSSS 120
           KREGRATATATATKGVPSFRYGGSMRENHDPLSFRPRSYDSGFSLSRNASRKSQNTHSSS
Sbjct: 61  KREGRATATATATKGVPSFRYGGSMRENHDPLSFRPRSYDSGFSLSRNASRKSQNTHSSS 120

Query: 121 PLFRSVSRRSTESSVPSRVSSGRRSVDLVSSPPLLSKSGSRRSTTPIMFSNSSGVLKPAP 180
           PLFRSVSRRSTESSVPSRVSSGRRSVDLVSSPPLLSKSGSRRSTTPIMFSNSSGVLKPAP
Sbjct: 121 PLFRSVSRRSTESSVPSRVSSGRRSVDLVSSPPLLSKSGSRRSTTPIMFSNSSGVLKPAP 180

Query: 181 IEKQLECTLEELCFGCTKKIKITRDVLSNTTGQTTEEEETLTINVKPGWKKGTKITFEGM 240
           IEKQLECTLEELCFGCTKKIKITRDVLSNTTGQTTEEEETLTINVKPGWKKGTKITFEGM
Sbjct: 181 IEKQLECTLEELCFGCTKKIKITRDVLSNTTGQTTEEEETLTINVKPGWKKGTKITFEGM 240

Query: 241 GNERPGSYPADMSFVIAEKRHAFFKWEGDDLEMTVEIPLLKALTGCTISVPLLGGEAMSL 300
           GNERPGSYPADMSFVIAEKRHAFFKWEGDDLEMTVEIPLLKALTGCTISVPLLGGEAMSL
Sbjct: 241 GNERPGSYPADMSFVIAEKRHAFFKWEGDDLEMTVEIPLLKALTGCTISVPLLGGEAMSL 300

Query: 301 EIDDVVSPGYERIIQGQGMPNPKDDDKRGDLKVKFLVEFPTQLTDQQRSDVCRILEDGCV 360
           EIDDVVSPGYERIIQGQGMPNPKDDDKRGDLKVKFLVEFPTQLTDQQRSDVCRILEDGCV
Sbjct: 301 EIDDVVSPGYERIIQGQGMPNPKDDDKRGDLKVKFLVEFPTQLTDQQRSDVCRILEDGCV 360

BLAST of MC11g0707 vs. NCBI nr
Match: XP_038899191.1 (dnaJ homolog subfamily B member 4-like [Benincasa hispida])

HSP 1 Score: 509 bits (1310), Expect = 2.65e-178
Identity = 285/364 (78.30%), Postives = 309/364 (84.89%), Query Frame = 0

Query: 1   MVDLPQAIGISFRNFCKSYKSFFKKWHSKIKSSKKDSDV-DQKREGSSDAI--DGPSKDR 60
           MVDLPQA+GISFRNFCKSYKSFFKKWHSK K+SKKD+DV D K + +SDAI  +GPSKD 
Sbjct: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKAKNSKKDADVVDTKHDATSDAIGNNGPSKDH 60

Query: 61  EDVKREGRATATATATKGVPSFRYGG-SMRENHDPLSFRPRSYDSGFS-LSRNASRKSQN 120
           E+VK+    T   T  K V S RYGG SMREN D  SFRPRSYDSG S LSRNASR+ QN
Sbjct: 61  EEVKK----TEGPTTMKSVHSLRYGGQSMREN-DATSFRPRSYDSGVSSLSRNASRRGQN 120

Query: 121 THS-SSPLFRSVSRRSTESSVPSRVSSGRRSVDLVSSPPLLSKSGSRRSTTPIMFSNSSG 180
             S SS LFRSVSRRSTES VPSRVSSGRRS+D VSS PLLSKSGSR STTPIMFSNSSG
Sbjct: 121 PSSTSSSLFRSVSRRSTES-VPSRVSSGRRSIDSVSSSPLLSKSGSRMSTTPIMFSNSSG 180

Query: 181 VLKPAPIEKQLECTLEELCFGCTKKIKITRDVLSNTTGQTTEEEETLTINVKPGWKKGTK 240
           +LKPAPIEKQLECTLEELCFGC KKIK+TRD++   +G+T EEEETLTI +KPGW KGTK
Sbjct: 181 LLKPAPIEKQLECTLEELCFGCMKKIKVTRDLIL-ISGKTMEEEETLTIKIKPGWTKGTK 240

Query: 241 ITFEGMGNERPGSYPADMSFVIAEKRHAFFKWEGDDLEMTVEIPLLKALTGCTISVPLLG 300
           ITFEGMGNERPGSYPADMSFVI EKRH +FK EGDDLE+TVEIPLLKAL GC ISVPLLG
Sbjct: 241 ITFEGMGNERPGSYPADMSFVIVEKRHPYFKREGDDLELTVEIPLLKALIGCNISVPLLG 300

Query: 301 GEAMSLEIDDVVSPGYERIIQGQGMPNPKDDDKRGDLKVKFLVEFPTQLTDQQRSDVCRI 358
           GE MSL+I DVV PGYE++IQGQGMP  KDD KRG+LKVKFLVEFPT+LTDQQR DVCRI
Sbjct: 301 GETMSLKIHDVVWPGYEKVIQGQGMPKLKDD-KRGNLKVKFLVEFPTELTDQQRYDVCRI 356

BLAST of MC11g0707 vs. NCBI nr
Match: XP_008444178.1 (PREDICTED: chaperone protein DnaJ [Cucumis melo])

HSP 1 Score: 489 bits (1259), Expect = 1.95e-170
Identity = 277/365 (75.89%), Postives = 306/365 (83.84%), Query Frame = 0

Query: 1   MVDLPQAIGISFRNFCKSYKSFFKKWHSKIKSSKKDSD--VDQKRE-GSSDAIDG--PSK 60
           MVDLPQA+GISFRNFCKSYKSFFKKWHSK+K+SKKD+D  VD K +  +SDAI    PSK
Sbjct: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKVKNSKKDTDNVVDTKHDDATSDAIHNHSPSK 60

Query: 61  DREDVKREGRATATATATKGVPSFRYGG-SMRENHDPLSFRPRSYDSGFS-LSRNASRKS 120
           D E++K++     T T T+GV SFRYGG S+REN D  SFRPRSYDSG+S LSRNASR+ 
Sbjct: 61  DHEEIKKKEGPRPTTT-TRGVHSFRYGGRSLREN-DTTSFRPRSYDSGYSTLSRNASRRG 120

Query: 121 QNTHS-SSPLFRSVSRRSTESSVPSRVSSGRRSVDLVSSPPLLSKSGSRRSTTPIMFSNS 180
           QN  S SS LFRSVSRRS ES +PSRVSSGRRS+D +SS P+LSKSGSRRSTTPIMFSNS
Sbjct: 121 QNPASTSSSLFRSVSRRSNES-MPSRVSSGRRSIDSISSSPVLSKSGSRRSTTPIMFSNS 180

Query: 181 SGVLKPAPIEKQLECTLEELCFGCTKKIKITRDVLSNTTGQTTEEEETLTINVKPGWKKG 240
           SGVLK APIEKQLECTLEELCFGC KKIK+TRD+L    GQ  EEEETLTI VKPGW+KG
Sbjct: 181 SGVLKAAPIEKQLECTLEELCFGCMKKIKVTRDLLL-INGQAMEEEETLTIKVKPGWRKG 240

Query: 241 TKITFEG-MGNERPGSYPADMSFVIAEKRHAFFKWEGDDLEMTVEIPLLKALTGCTISVP 300
           TKITFEG  GNER GSYPAD SFVIAEKRH++FK EGDDLE+ VEIPLLKALTGCTI VP
Sbjct: 241 TKITFEGGTGNERAGSYPADTSFVIAEKRHSYFKREGDDLELMVEIPLLKALTGCTIPVP 300

Query: 301 LLGGEAMSLEIDDVVSPGYERIIQGQGMPNPKDDDKRGDLKVKFLVEFPTQLTDQQRSDV 356
           LLGGE MSLEI +VVSPGYE++IQGQGMP  KD + RG+L VKF V FPTQLT QQRSDV
Sbjct: 301 LLGGETMSLEIHEVVSPGYEKLIQGQGMPKLKDPNNRGNLIVKFFVHFPTQLTPQQRSDV 360

BLAST of MC11g0707 vs. NCBI nr
Match: XP_004142703.1 (uncharacterized protein LOC101218142 [Cucumis sativus] >KGN54553.1 hypothetical protein Csa_012239 [Cucumis sativus])

HSP 1 Score: 486 bits (1250), Expect = 4.09e-169
Identity = 275/366 (75.14%), Postives = 306/366 (83.61%), Query Frame = 0

Query: 1   MVDLPQAIGISFRNFCKSYKSFFKKWHSKIKSSKKDSD---VDQKREG-SSDAID--GPS 60
           MVDLPQA+GISFRNFCKSYKSFFKKWHSK+K+SKKD+D   VD K +  +SDAI   GPS
Sbjct: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKVKNSKKDTDNVVVDTKHDDPTSDAIHNYGPS 60

Query: 61  KDREDVKREGRATATATATKGVPSFRYGG-SMRENHDPLSFRPRSYDSGFS-LSRNASRK 120
           KD ED+K++         TKGV SFRYGG S+REN D  SFRP+SYDSG+S LSRNASR+
Sbjct: 61  KDHEDIKKK----EGPRPTKGVHSFRYGGRSLREN-DTTSFRPQSYDSGYSTLSRNASRR 120

Query: 121 SQNTHS-SSPLFRSVSRRSTESSVPSRVSSGRRSVDLVSSPPLLSKSGSRRSTTPIMFSN 180
            QN  S SS LFRS+SRRS ES + SRVSSGRRS+D +SS PLLSKSGS+RSTTPIMFSN
Sbjct: 121 GQNAGSTSSSLFRSMSRRSNES-MTSRVSSGRRSIDSISSSPLLSKSGSKRSTTPIMFSN 180

Query: 181 SSGVLKPAPIEKQLECTLEELCFGCTKKIKITRDVLSNTTGQTTEEEETLTINVKPGWKK 240
           SSGVLK A IEKQLECTLEELCFGC KKIK+TRD+L    GQ  EEEETLT+ VKPGW+K
Sbjct: 181 SSGVLKAAAIEKQLECTLEELCFGCIKKIKVTRDLLL-INGQAMEEEETLTMKVKPGWRK 240

Query: 241 GTKITFEG-MGNERPGSYPADMSFVIAEKRHAFFKWEGDDLEMTVEIPLLKALTGCTISV 300
           GTKITFEG MGNER GSYPAD SFVIAEKRH++FK EGDDLE+ VEIPLLKALTGCTISV
Sbjct: 241 GTKITFEGGMGNERAGSYPADTSFVIAEKRHSYFKREGDDLELMVEIPLLKALTGCTISV 300

Query: 301 PLLGGEAMSLEIDDVVSPGYERIIQGQGMPNPKDDDKRGDLKVKFLVEFPTQLTDQQRSD 356
           PLLGGE MSL+I +VVSPGYE++IQGQGMP  KD D RGDL +KF V+FPTQLT QQRSD
Sbjct: 301 PLLGGETMSLDIHEVVSPGYEKLIQGQGMPKLKDPDTRGDLILKFFVDFPTQLTPQQRSD 359

BLAST of MC11g0707 vs. NCBI nr
Match: XP_022941087.1 (dnaJ homolog subfamily B member 5-like [Cucurbita moschata])

HSP 1 Score: 468 bits (1205), Expect = 2.38e-162
Identity = 263/363 (72.45%), Postives = 297/363 (81.82%), Query Frame = 0

Query: 1   MVDLPQAIGISFRNFCKSYKSFFKKWHSKIKSSKKDSDV-DQKREGSSDAID----GPSK 60
           MVDLPQ +G+SFRNFCKSYKSFFKKWHSK+K+SKK++DV D K +GSSDAID    GPSK
Sbjct: 1   MVDLPQVLGVSFRNFCKSYKSFFKKWHSKVKNSKKNTDVVDSKHDGSSDAIDNNNNGPSK 60

Query: 61  DREDVKREGRATATATATKGVPSFRYGG-SMRENHDPLSFRPRSYDSGFSLSRNASRKSQ 120
           DRE++ +EG    T    KG  SFRY   SMREN DP   R RSYDS  S+ RNASR+  
Sbjct: 61  DREEINKEGPRQMTK---KGGLSFRYASRSMREN-DPKISRHRSYDS--SMFRNASRRGL 120

Query: 121 NTH-SSSPLFRSVSRRSTESSVPSRVSSGRRSVDLVSSPPLLSKSGSRRSTTPIMFSNSS 180
           N   SSS  FRSVSRRSTES +PSR++SG  SVD  SS P+LSKSGSRRSTTPIMFSNSS
Sbjct: 121 NPQPSSSSQFRSVSRRSTES-MPSRLNSGL-SVDSASSSPMLSKSGSRRSTTPIMFSNSS 180

Query: 181 GVLKPAPIEKQLECTLEELCFGCTKKIKITRDVLSNTTGQTTEEEETLTINVKPGWKKGT 240
           GVLKP PIEKQLECTLEELCFGC KKIK+TRDV+ NT GQ  EEEET+TI VKPGW KGT
Sbjct: 181 GVLKPPPIEKQLECTLEELCFGCMKKIKVTRDVILNT-GQAIEEEETVTIRVKPGWTKGT 240

Query: 241 KITFEGMGNERPGSYPADMSFVIAEKRHAFFKWEGDDLEMTVEIPLLKALTGCTISVPLL 300
           KITFEGMGNERPGSYPAD+SFVI EK+H +FK EGDDL++TVEIPLLKALTGCTIS+PLL
Sbjct: 241 KITFEGMGNERPGSYPADISFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLL 300

Query: 301 GGEAMSLEIDDVVSPGYERIIQGQGMPNPKDDDKRGDLKVKFLVEFPTQLTDQQRSDVCR 356
           GG+ MSL   DV+  G  +II+GQGMP  K++  RG+LKVKFL+EFPT+LTDQQRSDVCR
Sbjct: 301 GGDVMSLRTHDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCR 354

BLAST of MC11g0707 vs. ExPASy TrEMBL
Match: A0A6J1BSE9 (uncharacterized protein LOC111005118 OS=Momordica charantia OX=3673 GN=LOC111005118 PE=4 SV=1)

HSP 1 Score: 704 bits (1817), Expect = 7.78e-256
Identity = 360/360 (100.00%), Postives = 360/360 (100.00%), Query Frame = 0

Query: 1   MVDLPQAIGISFRNFCKSYKSFFKKWHSKIKSSKKDSDVDQKREGSSDAIDGPSKDREDV 60
           MVDLPQAIGISFRNFCKSYKSFFKKWHSKIKSSKKDSDVDQKREGSSDAIDGPSKDREDV
Sbjct: 1   MVDLPQAIGISFRNFCKSYKSFFKKWHSKIKSSKKDSDVDQKREGSSDAIDGPSKDREDV 60

Query: 61  KREGRATATATATKGVPSFRYGGSMRENHDPLSFRPRSYDSGFSLSRNASRKSQNTHSSS 120
           KREGRATATATATKGVPSFRYGGSMRENHDPLSFRPRSYDSGFSLSRNASRKSQNTHSSS
Sbjct: 61  KREGRATATATATKGVPSFRYGGSMRENHDPLSFRPRSYDSGFSLSRNASRKSQNTHSSS 120

Query: 121 PLFRSVSRRSTESSVPSRVSSGRRSVDLVSSPPLLSKSGSRRSTTPIMFSNSSGVLKPAP 180
           PLFRSVSRRSTESSVPSRVSSGRRSVDLVSSPPLLSKSGSRRSTTPIMFSNSSGVLKPAP
Sbjct: 121 PLFRSVSRRSTESSVPSRVSSGRRSVDLVSSPPLLSKSGSRRSTTPIMFSNSSGVLKPAP 180

Query: 181 IEKQLECTLEELCFGCTKKIKITRDVLSNTTGQTTEEEETLTINVKPGWKKGTKITFEGM 240
           IEKQLECTLEELCFGCTKKIKITRDVLSNTTGQTTEEEETLTINVKPGWKKGTKITFEGM
Sbjct: 181 IEKQLECTLEELCFGCTKKIKITRDVLSNTTGQTTEEEETLTINVKPGWKKGTKITFEGM 240

Query: 241 GNERPGSYPADMSFVIAEKRHAFFKWEGDDLEMTVEIPLLKALTGCTISVPLLGGEAMSL 300
           GNERPGSYPADMSFVIAEKRHAFFKWEGDDLEMTVEIPLLKALTGCTISVPLLGGEAMSL
Sbjct: 241 GNERPGSYPADMSFVIAEKRHAFFKWEGDDLEMTVEIPLLKALTGCTISVPLLGGEAMSL 300

Query: 301 EIDDVVSPGYERIIQGQGMPNPKDDDKRGDLKVKFLVEFPTQLTDQQRSDVCRILEDGCV 360
           EIDDVVSPGYERIIQGQGMPNPKDDDKRGDLKVKFLVEFPTQLTDQQRSDVCRILEDGCV
Sbjct: 301 EIDDVVSPGYERIIQGQGMPNPKDDDKRGDLKVKFLVEFPTQLTDQQRSDVCRILEDGCV 360

BLAST of MC11g0707 vs. ExPASy TrEMBL
Match: A0A1S3BAK8 (chaperone protein DnaJ OS=Cucumis melo OX=3656 GN=LOC103487597 PE=4 SV=1)

HSP 1 Score: 489 bits (1259), Expect = 9.43e-171
Identity = 277/365 (75.89%), Postives = 306/365 (83.84%), Query Frame = 0

Query: 1   MVDLPQAIGISFRNFCKSYKSFFKKWHSKIKSSKKDSD--VDQKRE-GSSDAIDG--PSK 60
           MVDLPQA+GISFRNFCKSYKSFFKKWHSK+K+SKKD+D  VD K +  +SDAI    PSK
Sbjct: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKVKNSKKDTDNVVDTKHDDATSDAIHNHSPSK 60

Query: 61  DREDVKREGRATATATATKGVPSFRYGG-SMRENHDPLSFRPRSYDSGFS-LSRNASRKS 120
           D E++K++     T T T+GV SFRYGG S+REN D  SFRPRSYDSG+S LSRNASR+ 
Sbjct: 61  DHEEIKKKEGPRPTTT-TRGVHSFRYGGRSLREN-DTTSFRPRSYDSGYSTLSRNASRRG 120

Query: 121 QNTHS-SSPLFRSVSRRSTESSVPSRVSSGRRSVDLVSSPPLLSKSGSRRSTTPIMFSNS 180
           QN  S SS LFRSVSRRS ES +PSRVSSGRRS+D +SS P+LSKSGSRRSTTPIMFSNS
Sbjct: 121 QNPASTSSSLFRSVSRRSNES-MPSRVSSGRRSIDSISSSPVLSKSGSRRSTTPIMFSNS 180

Query: 181 SGVLKPAPIEKQLECTLEELCFGCTKKIKITRDVLSNTTGQTTEEEETLTINVKPGWKKG 240
           SGVLK APIEKQLECTLEELCFGC KKIK+TRD+L    GQ  EEEETLTI VKPGW+KG
Sbjct: 181 SGVLKAAPIEKQLECTLEELCFGCMKKIKVTRDLLL-INGQAMEEEETLTIKVKPGWRKG 240

Query: 241 TKITFEG-MGNERPGSYPADMSFVIAEKRHAFFKWEGDDLEMTVEIPLLKALTGCTISVP 300
           TKITFEG  GNER GSYPAD SFVIAEKRH++FK EGDDLE+ VEIPLLKALTGCTI VP
Sbjct: 241 TKITFEGGTGNERAGSYPADTSFVIAEKRHSYFKREGDDLELMVEIPLLKALTGCTIPVP 300

Query: 301 LLGGEAMSLEIDDVVSPGYERIIQGQGMPNPKDDDKRGDLKVKFLVEFPTQLTDQQRSDV 356
           LLGGE MSLEI +VVSPGYE++IQGQGMP  KD + RG+L VKF V FPTQLT QQRSDV
Sbjct: 301 LLGGETMSLEIHEVVSPGYEKLIQGQGMPKLKDPNNRGNLIVKFFVHFPTQLTPQQRSDV 360

BLAST of MC11g0707 vs. ExPASy TrEMBL
Match: A0A0A0KYJ4 (DnaJ_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G361870 PE=4 SV=1)

HSP 1 Score: 486 bits (1250), Expect = 1.98e-169
Identity = 275/366 (75.14%), Postives = 306/366 (83.61%), Query Frame = 0

Query: 1   MVDLPQAIGISFRNFCKSYKSFFKKWHSKIKSSKKDSD---VDQKREG-SSDAID--GPS 60
           MVDLPQA+GISFRNFCKSYKSFFKKWHSK+K+SKKD+D   VD K +  +SDAI   GPS
Sbjct: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKVKNSKKDTDNVVVDTKHDDPTSDAIHNYGPS 60

Query: 61  KDREDVKREGRATATATATKGVPSFRYGG-SMRENHDPLSFRPRSYDSGFS-LSRNASRK 120
           KD ED+K++         TKGV SFRYGG S+REN D  SFRP+SYDSG+S LSRNASR+
Sbjct: 61  KDHEDIKKK----EGPRPTKGVHSFRYGGRSLREN-DTTSFRPQSYDSGYSTLSRNASRR 120

Query: 121 SQNTHS-SSPLFRSVSRRSTESSVPSRVSSGRRSVDLVSSPPLLSKSGSRRSTTPIMFSN 180
            QN  S SS LFRS+SRRS ES + SRVSSGRRS+D +SS PLLSKSGS+RSTTPIMFSN
Sbjct: 121 GQNAGSTSSSLFRSMSRRSNES-MTSRVSSGRRSIDSISSSPLLSKSGSKRSTTPIMFSN 180

Query: 181 SSGVLKPAPIEKQLECTLEELCFGCTKKIKITRDVLSNTTGQTTEEEETLTINVKPGWKK 240
           SSGVLK A IEKQLECTLEELCFGC KKIK+TRD+L    GQ  EEEETLT+ VKPGW+K
Sbjct: 181 SSGVLKAAAIEKQLECTLEELCFGCIKKIKVTRDLLL-INGQAMEEEETLTMKVKPGWRK 240

Query: 241 GTKITFEG-MGNERPGSYPADMSFVIAEKRHAFFKWEGDDLEMTVEIPLLKALTGCTISV 300
           GTKITFEG MGNER GSYPAD SFVIAEKRH++FK EGDDLE+ VEIPLLKALTGCTISV
Sbjct: 241 GTKITFEGGMGNERAGSYPADTSFVIAEKRHSYFKREGDDLELMVEIPLLKALTGCTISV 300

Query: 301 PLLGGEAMSLEIDDVVSPGYERIIQGQGMPNPKDDDKRGDLKVKFLVEFPTQLTDQQRSD 356
           PLLGGE MSL+I +VVSPGYE++IQGQGMP  KD D RGDL +KF V+FPTQLT QQRSD
Sbjct: 301 PLLGGETMSLDIHEVVSPGYEKLIQGQGMPKLKDPDTRGDLILKFFVDFPTQLTPQQRSD 359

BLAST of MC11g0707 vs. ExPASy TrEMBL
Match: A0A6J1FLF5 (dnaJ homolog subfamily B member 5-like OS=Cucurbita moschata OX=3662 GN=LOC111446483 PE=4 SV=1)

HSP 1 Score: 468 bits (1205), Expect = 1.15e-162
Identity = 263/363 (72.45%), Postives = 297/363 (81.82%), Query Frame = 0

Query: 1   MVDLPQAIGISFRNFCKSYKSFFKKWHSKIKSSKKDSDV-DQKREGSSDAID----GPSK 60
           MVDLPQ +G+SFRNFCKSYKSFFKKWHSK+K+SKK++DV D K +GSSDAID    GPSK
Sbjct: 1   MVDLPQVLGVSFRNFCKSYKSFFKKWHSKVKNSKKNTDVVDSKHDGSSDAIDNNNNGPSK 60

Query: 61  DREDVKREGRATATATATKGVPSFRYGG-SMRENHDPLSFRPRSYDSGFSLSRNASRKSQ 120
           DRE++ +EG    T    KG  SFRY   SMREN DP   R RSYDS  S+ RNASR+  
Sbjct: 61  DREEINKEGPRQMTK---KGGLSFRYASRSMREN-DPKISRHRSYDS--SMFRNASRRGL 120

Query: 121 NTH-SSSPLFRSVSRRSTESSVPSRVSSGRRSVDLVSSPPLLSKSGSRRSTTPIMFSNSS 180
           N   SSS  FRSVSRRSTES +PSR++SG  SVD  SS P+LSKSGSRRSTTPIMFSNSS
Sbjct: 121 NPQPSSSSQFRSVSRRSTES-MPSRLNSGL-SVDSASSSPMLSKSGSRRSTTPIMFSNSS 180

Query: 181 GVLKPAPIEKQLECTLEELCFGCTKKIKITRDVLSNTTGQTTEEEETLTINVKPGWKKGT 240
           GVLKP PIEKQLECTLEELCFGC KKIK+TRDV+ NT GQ  EEEET+TI VKPGW KGT
Sbjct: 181 GVLKPPPIEKQLECTLEELCFGCMKKIKVTRDVILNT-GQAIEEEETVTIRVKPGWTKGT 240

Query: 241 KITFEGMGNERPGSYPADMSFVIAEKRHAFFKWEGDDLEMTVEIPLLKALTGCTISVPLL 300
           KITFEGMGNERPGSYPAD+SFVI EK+H +FK EGDDL++TVEIPLLKALTGCTIS+PLL
Sbjct: 241 KITFEGMGNERPGSYPADISFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLL 300

Query: 301 GGEAMSLEIDDVVSPGYERIIQGQGMPNPKDDDKRGDLKVKFLVEFPTQLTDQQRSDVCR 356
           GG+ MSL   DV+  G  +II+GQGMP  K++  RG+LKVKFL+EFPT+LTDQQRSDVCR
Sbjct: 301 GGDVMSLRTHDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCR 354

BLAST of MC11g0707 vs. ExPASy TrEMBL
Match: A0A6J1ISU0 (dnaJ homolog subfamily B member 1-like OS=Cucurbita maxima OX=3661 GN=LOC111480292 PE=4 SV=1)

HSP 1 Score: 466 bits (1199), Expect = 9.40e-162
Identity = 262/363 (72.18%), Postives = 298/363 (82.09%), Query Frame = 0

Query: 1   MVDLPQAIGISFRNFCKSYKSFFKKWHSKIKSSKKDSDV-DQKREGSSDAID----GPSK 60
           MVDLPQ +G+SFRNFCKSYKSFFKKWHSK+K+SKK++DV D K +GSSDAID    GPSK
Sbjct: 1   MVDLPQVLGVSFRNFCKSYKSFFKKWHSKVKNSKKNTDVVDSKHDGSSDAIDNNNNGPSK 60

Query: 61  DREDVKREGRATATATATKGVPSFRYGG-SMRENHDPLSFRPRSYDSGFSLSRNASRKSQ 120
           DRE++ +EG    T    KG  SFRYG  SMREN DP   R RSYDS  S+ RNASR+  
Sbjct: 61  DREEISKEGPRQMTK---KGGLSFRYGSRSMREN-DPKISRHRSYDS--SMFRNASRRGL 120

Query: 121 NTH-SSSPLFRSVSRRSTESSVPSRVSSGRRSVDLVSSPPLLSKSGSRRSTTPIMFSNSS 180
           N   SSS  FRSVSRRSTES +PSR++SG  SVD  SS P+LSKSGSRRSTTPIMFSNSS
Sbjct: 121 NPQPSSSSQFRSVSRRSTES-MPSRLNSGL-SVDSASSSPMLSKSGSRRSTTPIMFSNSS 180

Query: 181 GVLKPAPIEKQLECTLEELCFGCTKKIKITRDVLSNTTGQTTEEEETLTINVKPGWKKGT 240
           GVLKPAPIEKQLECTLEELCFGC KKIK+TRDV+ NT GQ  EEEET+TI VKPGW KGT
Sbjct: 181 GVLKPAPIEKQLECTLEELCFGCMKKIKVTRDVILNT-GQAIEEEETVTIRVKPGWTKGT 240

Query: 241 KITFEGMGNERPGSYPADMSFVIAEKRHAFFKWEGDDLEMTVEIPLLKALTGCTISVPLL 300
           KITFEGMGNER GSYPAD+SFVI EK+H +FK EGDDL++TVEIPL+KALTGCTIS+PLL
Sbjct: 241 KITFEGMGNERLGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPLVKALTGCTISIPLL 300

Query: 301 GGEAMSLEIDDVVSPGYERIIQGQGMPNPKDDDKRGDLKVKFLVEFPTQLTDQQRSDVCR 356
           GG+ MSL+  DV+  G  +II+GQGMP  K++  RG+LKVKFL EFPT+LTDQQRSD+CR
Sbjct: 301 GGDIMSLKTQDVIWHGCVKIIKGQGMPKLKEEINRGNLKVKFLTEFPTELTDQQRSDICR 354

BLAST of MC11g0707 vs. TAIR 10
Match: AT1G11040.1 (HSP40/DnaJ peptide-binding protein )

HSP 1 Score: 252.7 bits (644), Expect = 4.5e-67
Identity = 138/278 (49.64%), Postives = 192/278 (69.06%), Query Frame = 0

Query: 83  GSMRENHDPLSFRPRS---YDSGFSLSRNASRKSQNTHSSSPLFRSVSRRSTESSVPSRV 142
           GS+++   P S  P +     S  S+S++AS++ ++   S       +  +T +S+P   
Sbjct: 170 GSVKKLSSPTSNSPINGVKQSSPSSISKSASKRDKDERGS-------TSSATSTSLPYSK 229

Query: 143 SSGRRSVDLVSSPPLLSKSGSRRSTTPIMFSNSSGVLKPAPIEKQLECTLEELCFGCTKK 202
           S   R  D   S   ++KS SRRSTTPI+FS S+   KP  +EK+LECTLEELC G  K 
Sbjct: 230 SKSTR--DTAGS---IAKSISRRSTTPIVFSQSTPPKKPPAVEKKLECTLEELCHGGVKN 289

Query: 203 IKITRDVLSNTTGQTTEEEETLTINVKPGWKKGTKITFEGMGNERPGSYPADMSFVIAEK 262
           IKI RD++++  G   ++EE L +N++PGWKKGTKITFEG+GNE+PG  P D++FV+ EK
Sbjct: 290 IKIKRDIITD-EGLIMQQEEMLRVNIQPGWKKGTKITFEGVGNEKPGYLPEDITFVVEEK 349

Query: 263 RHAFFKWEGDDLEMTVEIPLLKALTGCTISVPLLGGEAMSLEIDDVVSPGYERIIQGQGM 322
           RH  FK  GDDLE+ VEIPLLKALTGC +SVPLL GE+MS+ + DV+  G+E+ I+GQGM
Sbjct: 350 RHPLFKRRGDDLEIAVEIPLLKALTGCKLSVPLLSGESMSITVGDVIFHGFEKAIKGQGM 409

Query: 323 PNPKDDDKRGDLKVKFLVEFPTQLTDQQRSDVCRILED 358
           PN K++ KRGDL++ FLV FP +L+++QRS    +L+D
Sbjct: 410 PNAKEEGKRGDLRITFLVNFPEKLSEEQRSMAYEVLKD 434

BLAST of MC11g0707 vs. TAIR 10
Match: AT1G44160.1 (HSP40/DnaJ peptide-binding protein )

HSP 1 Score: 243.8 bits (621), Expect = 2.1e-64
Identity = 134/258 (51.94%), Postives = 175/258 (67.83%), Query Frame = 0

Query: 104 SLSRNASRKSQNTHSSSPLFRSVSRRSTESSVPSRVSSGRRSVDLVSSPPLLSKSGS--- 163
           SLSR+ S+ +    S++P  RS+      S +    SS  R  +     P L +S +   
Sbjct: 105 SLSRSCSQNTATATSTNPTLRSL------SFIGRSKSSSNRMTESGGFMPTLMRSTTTVP 164

Query: 164 RRSTTPIMFSNSSG-VLKPAPIEKQLECTLEELCFGCTKKIKITRDVLSNTTGQTTEEEE 223
           R    PI++S+SS  V KP+P EK+L CTLEELC GCTKKIKI RDV++ + G+  EEEE
Sbjct: 165 RSFANPILYSSSSAKVAKPSPTEKKLRCTLEELCNGCTKKIKIKRDVIT-SLGEKCEEEE 224

Query: 224 TLTINVKPGWKKGTKITFEGMGNERPGSYPADMSFVIAEKRHAFFKWEGDDLEMTVEIPL 283
            + I VKPGWK GTK+TFEG GNE   S PAD++FVI EK H  FK EGDDLEM VE+ L
Sbjct: 225 MVEIKVKPGWKGGTKVTFEGKGNEAMRSVPADLTFVIVEKEHEVFKREGDDLEMAVEVSL 284

Query: 284 LKALTGCTISVPLLGGEAMSLEIDDVVSPGYERIIQGQGMPNPKDDDKRGDLKVKFLVEF 343
           L+ALTGC +SV LL G+ M L I+DV+ PGY  ++QG+GMPN K+  KRGDL+V+F  +F
Sbjct: 285 LEALTGCELSVALLDGDNMRLRIEDVIHPGYVTVVQGKGMPNLKEKGKRGDLRVRFRTKF 344

Query: 344 PTQLTDQQRSDVCRILED 358
           P  LTD+QR+++  IL+D
Sbjct: 345 PQHLTDEQRAEIHSILQD 355

BLAST of MC11g0707 vs. TAIR 10
Match: AT3G08910.1 (DNAJ heat shock family protein )

HSP 1 Score: 190.7 bits (483), Expect = 2.1e-48
Identity = 129/340 (37.94%), Postives = 190/340 (55.88%), Query Frame = 0

Query: 17  KSYKSFFKKWH-SKIKSSKKDSDVDQKREGSS-DAIDGPSKDREDVKREGRATATATATK 76
           K+Y+    KWH  K  ++KKD++   K+   + D +  P K R    + G    T+ A  
Sbjct: 22  KAYRKLAMKWHPDKNPNNKKDAEAKFKQISEAYDVLSDPQK-RAIYDQYGEEGLTSQA-- 81

Query: 77  GVPSFRYGGSMRENHDPLSFRPRSYDSGFSLSRNASRKSQNTHSSSPLFRSVSRRSTESS 136
             P    GG   +      F  RS D  FS     +R   ++  + P             
Sbjct: 82  --PPPGAGGGFSDGGASFRFNGRSADDIFSEFFGFTRPFGDSRGAGP------------- 141

Query: 137 VPSRVSSGRRSVDLVSSPPLLSKSGSRRSTTPIMFSNSSGVLKPAPIEKQLECTLEELCF 196
                S+G R  + V S                  SN     K APIE+QL C+LE+L  
Sbjct: 142 -----SNGFRFAEDVFS------------------SNVVPPRKAAPIERQLPCSLEDLYK 201

Query: 197 GCTKKIKITRDVLSNTTGQTTEEEETLTINVKPGWKKGTKITFEGMGNERPGSYPADMSF 256
           G +KK+KI+RDVL +++G+ T  EE LTI +KPGWKKGTKITF   GNE+ G  P+D+ F
Sbjct: 202 GVSKKMKISRDVL-DSSGRPTTVEEILTIEIKPGWKKGTKITFPEKGNEQRGIIPSDLVF 261

Query: 257 VIAEKRHAFFKWEGDDLEMTVEIPLLKALTGCTISVPLLGGEAMSLEIDDVVSPGYERII 316
           ++ EK HA FK +G+DL MT +IPL++ALTG T  V  L G ++++ I++V+SP YE ++
Sbjct: 262 IVDEKPHAVFKRDGNDLVMTQKIPLVEALTGYTAQVSTLDGRSVTVPINNVISPSYEEVV 319

Query: 317 QGQGMPNPKDDDKRGDLKVKFLVEFPTQLTDQQRSDVCRI 355
           +G+GMP PKD  K+G+L++KF V+FP++LT +Q+S + R+
Sbjct: 322 KGEGMPIPKDPSKKGNLRIKFTVKFPSRLTTEQKSGIKRM 319

BLAST of MC11g0707 vs. TAIR 10
Match: AT2G20560.1 (DNAJ heat shock family protein )

HSP 1 Score: 186.0 bits (471), Expect = 5.2e-47
Identity = 89/182 (48.90%), Postives = 133/182 (73.08%), Query Frame = 0

Query: 174 GVLKPAPIEKQLECTLEELCFGCTKKIKITRDVLSNTTGQTTEEEETLTINVKPGWKKGT 233
           G  K APIE +L C+LE+L  G TKK++I+R++ ++ +G+T + EE LTI+VKPGWKKGT
Sbjct: 156 GARKAAPIENKLPCSLEDLYKGTTKKMRISREI-ADVSGKTMQVEEILTIDVKPGWKKGT 215

Query: 234 KITFEGMGNERPGSYPADMSFVIAEKRHAFFKWEGDDLEMTVEIPLLKALTGCTISVPLL 293
           KITF   GNE+PG  PAD+ F+I EK H  F  EG+DL +T +I L++ALTG T+++  L
Sbjct: 216 KITFPEKGNEQPGVIPADLVFIIDEKPHPVFTREGNDLIVTQKISLVEALTGYTVNLTTL 275

Query: 294 GGEAMSLEIDDVVSPGYERIIQGQGMPNPKDDDKRGDLKVKFLVEFPTQLTDQQRSDVCR 353
            G  +++ + +VV P YE ++  +GMP  KD  KRG+L++KF ++FPT+LT +Q++ V +
Sbjct: 276 DGRRLTIPVTNVVHPEYEEVVPKEGMPLQKDQTKRGNLRIKFNIKFPTRLTSEQKTGVKK 335

Query: 354 IL 356
           +L
Sbjct: 336 LL 336

BLAST of MC11g0707 vs. TAIR 10
Match: AT2G20550.1 (HSP40/DnaJ peptide-binding protein )

HSP 1 Score: 179.1 bits (453), Expect = 6.3e-45
Identity = 83/182 (45.60%), Postives = 129/182 (70.88%), Query Frame = 0

Query: 174 GVLKPAPIEKQLECTLEELCFGCTKKIKITRDVLSNTTGQTTEEEETLTINVKPGWKKGT 233
           G  K AP+EK+L C+LE+L  G TKK+KI+R++ +   G+TT+ +E LT++VKPGWK GT
Sbjct: 103 GARKAAPVEKKLPCSLEDLYKGTTKKMKISREI-AGVFGKTTQVQEILTVDVKPGWKTGT 162

Query: 234 KITFEGMGNERPGSYPADMSFVIAEKRHAFFKWEGDDLEMTVEIPLLKALTGCTISVPLL 293
           KITF   GNE+PG  PAD+ F+I EK H  F  EG+DL +T +I +L+A TG T+++  L
Sbjct: 163 KITFSEKGNEQPGVIPADLVFIIDEKPHPVFTREGNDLVVTQKISVLEAFTGYTVNLTTL 222

Query: 294 GGEAMSLEIDDVVSPGYERIIQGQGMPNPKDDDKRGDLKVKFLVEFPTQLTDQQRSDVCR 353
            G  +++ ++ V+ P Y  ++  +GMP  KD  K+G+L++KF ++FPT LT +Q++ + +
Sbjct: 223 DGRRLTIPVNTVIHPEYVEVVPNEGMPLQKDQAKKGNLRIKFNIKFPTTLTSEQKTGLKK 282

Query: 354 IL 356
           +L
Sbjct: 283 LL 283

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q80Y752.3e-3946.75DnaJ homolog subfamily B member 13 OS=Mus musculus OX=10090 GN=Dnajb13 PE=1 SV=1[more]
P599108.6e-3946.02DnaJ homolog subfamily B member 13 OS=Homo sapiens OX=9606 GN=DNAJB13 PE=1 SV=1[more]
Q9UDY41.5e-3543.20DnaJ homolog subfamily B member 4 OS=Homo sapiens OX=9606 GN=DNAJB4 PE=1 SV=1[more]
Q5R8J81.5e-3543.20DnaJ homolog subfamily B member 4 OS=Pongo abelii OX=9601 GN=DNAJB4 PE=2 SV=1[more]
Q2KIT42.6e-3543.79DnaJ homolog subfamily B member 4 OS=Bos taurus OX=9913 GN=DNAJB4 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022132204.11.61e-255100.00uncharacterized protein LOC111005118 [Momordica charantia][more]
XP_038899191.12.65e-17878.30dnaJ homolog subfamily B member 4-like [Benincasa hispida][more]
XP_008444178.11.95e-17075.89PREDICTED: chaperone protein DnaJ [Cucumis melo][more]
XP_004142703.14.09e-16975.14uncharacterized protein LOC101218142 [Cucumis sativus] >KGN54553.1 hypothetical ... [more]
XP_022941087.12.38e-16272.45dnaJ homolog subfamily B member 5-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1BSE97.78e-256100.00uncharacterized protein LOC111005118 OS=Momordica charantia OX=3673 GN=LOC111005... [more]
A0A1S3BAK89.43e-17175.89chaperone protein DnaJ OS=Cucumis melo OX=3656 GN=LOC103487597 PE=4 SV=1[more]
A0A0A0KYJ41.98e-16975.14DnaJ_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G361870 PE=4... [more]
A0A6J1FLF51.15e-16272.45dnaJ homolog subfamily B member 5-like OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1ISU09.40e-16272.18dnaJ homolog subfamily B member 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114802... [more]
Match NameE-valueIdentityDescription
AT1G11040.14.5e-6749.64HSP40/DnaJ peptide-binding protein [more]
AT1G44160.12.1e-6451.94HSP40/DnaJ peptide-binding protein [more]
AT3G08910.12.1e-4837.94DNAJ heat shock family protein [more]
AT2G20560.15.2e-4748.90DNAJ heat shock family protein [more]
AT2G20550.16.3e-4545.60HSP40/DnaJ peptide-binding protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D2.60.260.20coord: 261..357
e-value: 2.0E-24
score: 87.6
NoneNo IPR availableGENE3D2.60.260.20coord: 177..260
e-value: 6.8E-31
score: 108.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 28..166
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 31..65
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 99..166
NoneNo IPR availablePANTHERPTHR24078:SF535DNAJ HOMOLOG SUBFAMILY B MEMBER 13-LIKEcoord: 69..356
NoneNo IPR availablePANTHERPTHR24078DNAJ HOMOLOG SUBFAMILY C MEMBERcoord: 69..356
NoneNo IPR availableCDDcd10747DnaJ_Ccoord: 179..343
e-value: 1.76442E-46
score: 153.735
IPR002939Chaperone DnaJ, C-terminalPFAMPF01556DnaJ_Ccoord: 181..340
e-value: 3.3E-42
score: 144.2
IPR008971HSP40/DnaJ peptide-bindingSUPERFAMILY49493HSP40/DnaJ peptide-binding domaincoord: 181..261
IPR008971HSP40/DnaJ peptide-bindingSUPERFAMILY49493HSP40/DnaJ peptide-binding domaincoord: 263..349

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC11g0707.1MC11g0707.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051085 chaperone cofactor-dependent protein refolding
biological_process GO:0006457 protein folding
cellular_component GO:0005829 cytosol
molecular_function GO:0051087 chaperone binding
molecular_function GO:0051082 unfolded protein binding