MC11g0678 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC11g0678
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	(1->3)-beta-glucan endohydrolase
Location	MC11: 5402743 .. 5404456 (+)
RNA-Seq Expression	MC11g0678
Synteny	MC11g0678

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGCGGCATTGGAGTGAACTACGGGCTCAACGGCAATAATCTCCCGCAAGCAAACGATGTGATAAACCTGTACGGACGATGCGGCATAGATATCGTGCGGATATTTGAGCCAAACCACTACGTTTTGGATGCGTTGCGCGGCAAACCAAATGTCTTGTGGCTCGGAACCAGAAATGAAGACATACAGAGCTTCGCTGCGGACCAATTGGCTGCCAATGCTTGGGTTAACTCTCACGTGGTTCCTTATTATGCCGACGTCAATATTGCTTACATCACCGTCGGTAATGAAGTCATTCCCGGGGACGCAGCGGCACCCTTCGTTGCTAATGCCATCAGAAACATTATGCAGGCGCTCGTTGTCACCGGCGTCAAACGAGACATAAAGGTAATATTTGAAATCAAGTTAAATTACGAATTTGATCCATATTATTATTTTAATTTCATTAGTTTACTTATGCGACATTTAATACATAAGTAGATCCGACTAAAAATTGTTTGAGTAATTAAGAATATGTCACTTTAGAAAATTAATAAGAGCTCGTGATCAATGTTTAAGCAAATATTTTTCAAACGAATCATTGTAATTTTAAAAAGTTTCAATTTTGATCTCTACAATTTGAATTTTCAATTTAGTCTAATATGGTTTCAAAATTTTCAATTTCGTCACTATAATTTAGTCATGGTTCAATCTAGTTTTCATAGTTTTAAAAGTTAAATTTCAATTTTATTTTTATGGTTTGGGTTTAATCTAATCCCTTTAATTTTAAAAGTTTCAAAAATAGTATAAAGTGTTATTATTATTACCATATATGAACATGTGTATGACATGTTTATTATTATTATTACTATTTTTGCAATGAATTATTGTTTTCTATTTATAGATAACCATATTTGTTAACATAGTATTAGCTAAAAGAAGTAAGGAACAGTAAATGGGTGGGTATTTTACCGAGTAAGGCAAATAGTTTTTCGATAACTTTTGGAGGTTTAACCTAAAAATAATTATCAAATTAAAACTTTTAAAATTTTGTTGGTGTGGATTAAATTGAAATTAAGCGAAATTCATATCCACAAATTCGTAATTTAATCTTAAAACCATATAAACTTGTTATATATATATAGTCTTTTATATTTATCCATAAATTATCAATTTCTTTGCACAGGTAACAACGGTGGTCCACATGAGTGTCATTGGAGTTTCGTATCCCCCATCGACTGGAGCATTCACCGATGCTGCTGCAGGACCTTTGAGCGAAATCGCAAAGGTGGTGGGCTCTAGCGGTGCACCATTATTGTTGAATGTATACCCATATTTCGCGTACGCGTCGAACCCACAACAAATCCCACTAAATTACGCCCTGTTCACTTCCACGACGCCGGTAGTGGTGGATGGAAATTTGAACTACTACAATCTATTTGATGCCATGGTGGATTCTTTCTATGCGGCGCTGGAGAAGATTGGCGCTGTTGATGTGAGACTGGAGATTTCGGAGACTGGGTGGCCCACAAAAGGAAACCAACCATTTACTAGTATTGATAATGCAGTGACGTATAACAAAAATTTCATGAACCATGTAGCGAGTGGTGCAGGAACTCCAAAGAGGCCAAAGGTGAAGTATAATGGGATCTTGTTTGAAATGTTCAACGAAGATCTTAAGTTGGCTGGAGTTGAACAGAATTTTGGATTCTTTTATCCCAATATGCAGCCAGTTTAT

mRNA sequence

GGCGGCATTGGAGTGAACTACGGGCTCAACGGCAATAATCTCCCGCAAGCAAACGATGTGATAAACCTGTACGGACGATGCGGCATAGATATCGTGCGGATATTTGAGCCAAACCACTACGTTTTGGATGCGTTGCGCGGCAAACCAAATGTCTTGTGGCTCGGAACCAGAAATGAAGACATACAGAGCTTCGCTGCGGACCAATTGGCTGCCAATGCTTGGGTTAACTCTCACGTGGTTCCTTATTATGCCGACGTCAATATTGCTTACATCACCGTCGGTAATGAAGTCATTCCCGGGGACGCAGCGGCACCCTTCGTTGCTAATGCCATCAGAAACATTATGCAGGCGCTCGTTGTCACCGGCGTCAAACGAGACATAAAGGTAACAACGGTGGTCCACATGAGTGTCATTGGAGTTTCGTATCCCCCATCGACTGGAGCATTCACCGATGCTGCTGCAGGACCTTTGAGCGAAATCGCAAAGGTGGTGGGCTCTAGCGGTGCACCATTATTGTTGAATGTATACCCATATTTCGCGTACGCGTCGAACCCACAACAAATCCCACTAAATTACGCCCTGTTCACTTCCACGACGCCGGTAGTGGTGGATGGAAATTTGAACTACTACAATCTATTTGATGCCATGGTGGATTCTTTCTATGCGGCGCTGGAGAAGATTGGCGCTGTTGATGTGAGACTGGAGATTTCGGAGACTGGGTGGCCCACAAAAGGAAACCAACCATTTACTAGTATTGATAATGCAGTGACGTATAACAAAAATTTCATGAACCATGTAGCGAGTGGTGCAGGAACTCCAAAGAGGCCAAAGGTGAAGTATAATGGGATCTTGTTTGAAATGTTCAACGAAGATCTTAAGTTGGCTGGAGTTGAACAGAATTTTGGATTCTTTTATCCCAATATGCAGCCAGTTTAT

Coding sequence (CDS)

GGCGGCATTGGAGTGAACTACGGGCTCAACGGCAATAATCTCCCGCAAGCAAACGATGTGATAAACCTGTACGGACGATGCGGCATAGATATCGTGCGGATATTTGAGCCAAACCACTACGTTTTGGATGCGTTGCGCGGCAAACCAAATGTCTTGTGGCTCGGAACCAGAAATGAAGACATACAGAGCTTCGCTGCGGACCAATTGGCTGCCAATGCTTGGGTTAACTCTCACGTGGTTCCTTATTATGCCGACGTCAATATTGCTTACATCACCGTCGGTAATGAAGTCATTCCCGGGGACGCAGCGGCACCCTTCGTTGCTAATGCCATCAGAAACATTATGCAGGCGCTCGTTGTCACCGGCGTCAAACGAGACATAAAGGTAACAACGGTGGTCCACATGAGTGTCATTGGAGTTTCGTATCCCCCATCGACTGGAGCATTCACCGATGCTGCTGCAGGACCTTTGAGCGAAATCGCAAAGGTGGTGGGCTCTAGCGGTGCACCATTATTGTTGAATGTATACCCATATTTCGCGTACGCGTCGAACCCACAACAAATCCCACTAAATTACGCCCTGTTCACTTCCACGACGCCGGTAGTGGTGGATGGAAATTTGAACTACTACAATCTATTTGATGCCATGGTGGATTCTTTCTATGCGGCGCTGGAGAAGATTGGCGCTGTTGATGTGAGACTGGAGATTTCGGAGACTGGGTGGCCCACAAAAGGAAACCAACCATTTACTAGTATTGATAATGCAGTGACGTATAACAAAAATTTCATGAACCATGTAGCGAGTGGTGCAGGAACTCCAAAGAGGCCAAAGGTGAAGTATAATGGGATCTTGTTTGAAATGTTCAACGAAGATCTTAAGTTGGCTGGAGTTGAACAGAATTTTGGATTCTTTTATCCCAATATGCAGCCAGTTTAT

Protein sequence

GGIGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPNVLWLGTRNEDIQSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRNIMQALVVTGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLLNVYPYFAYASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVRLEISETGWPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLKLAGVEQNFGFFYPNMQPVY

Homology

[Close]

BLAST of MC11g0678 vs. ExPASy Swiss-Prot
Match: O22317 (Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2)

HSP 1 Score: 303.5 bits (776), Expect = 2.7e-81
Identity = 153/310 (49.35%), Postives = 204/310 (65.81%), Query Frame = 0


Query: 3   IGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPNVLWLGTRNEDIQ 62
           IGV YG+ GNNLP  ++V++LY    I  +R+++PN   L ALR     + L     D+Q
Sbjct: 29  IGVCYGMLGNNLPPPSEVVSLYKSNNIARMRLYDPNQAALQALRNSNIQVLLDVPRSDVQ 88

Query: 63  SFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRNIMQALVVTG 122
           S A++  AA  W+  +VV Y+  V+  YI VGNE+IPG   A ++  A+RNI  AL   G
Sbjct: 89  SLASNPSAAGDWIRRNVVAYWPSVSFRYIAVGNELIPGSDLAQYILPAMRNIYNALSSAG 148

Query: 123 VKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLLNVYPYFAYA 182
           ++  IKV+T V   V+G SYPPS GAF+ AA   LS I + + S+GAPLL+NVYPYF+Y 
Sbjct: 149 LQNQIKVSTAVDTGVLGTSYPPSAGAFSSAAQAYLSPIVQFLASNGAPLLVNVYPYFSYT 208

Query: 183 SNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVRLEISETGWP 242
            NP QI L YALFT++  VV DG  +Y NLFDA+VD+ +AALE++G  +V + +SE+GWP
Sbjct: 209 GNPGQISLPYALFTASGVVVQDGRFSYQNLFDAIVDAVFAALERVGGANVAVVVSESGWP 268

Query: 243 TKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLKLAGVEQNFG 302
           + G     S  NA TYN+N + HV  G GTP+RP  +    +FEMFNE+ K  G+EQNFG
Sbjct: 269 SAGGGAEASTSNARTYNQNLIRHV--GGGTPRRPGKEIEAYIFEMFNENQKAGGIEQNFG 328

Query: 303 FFYPNMQPVY 313
            FYPN QPVY
Sbjct: 329 LFYPNKQPVY 336

[Close]

BLAST of MC11g0678 vs. ExPASy Swiss-Prot
Match: O49352 (Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN=BG4 PE=2 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 1.7e-75
Identity = 149/314 (47.45%), Postives = 208/314 (66.24%), Query Frame = 0


Query: 3   IGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPNV-LWLGTRNEDI 62
           IG+NYGL G+NLP  ++VINLY   GI  +RIF+PN  VL+ALRG  ++ + +G +++D+
Sbjct: 34  IGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTVGVKDQDL 93

Query: 63  QSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRNIMQAL--V 122
            + AA + A   W  +++  Y ADVNI +ITVGNEVIPG      +   +  +MQ+L  +
Sbjct: 94  AALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGP-----IGPQVLPVMQSLTNL 153

Query: 123 VTGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLLNVYPYF 182
           V      I ++TVV MS +  SYPPS G FT  A   L  + K++  +  P+L+N+YPYF
Sbjct: 154 VKSRNLPISISTVVAMSNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNIYPYF 213

Query: 183 AYASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVRLEISET 242
           AYAS+P  I L+YA F + + VV DG+L Y N+FDA+ D+F  A+EK G  ++ + +SET
Sbjct: 214 AYASDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPMVVSET 273

Query: 243 GWPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLKLAGVEQ 302
           GWP+ GN  FT+   A TYN+NF+ H+ASG GTPKRP    NG LF  FNE+ K AG EQ
Sbjct: 274 GWPSAGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFNENQKPAGTEQ 333

Query: 303 NFGFFYP-NMQPVY 313
           NFG + P +M+P+Y
Sbjct: 334 NFGLYNPSDMKPIY 342

[Close]

BLAST of MC11g0678 vs. ExPASy Swiss-Prot
Match: O49353 (Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN=BG5 PE=2 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 6.1e-73
Identity = 144/314 (45.86%), Postives = 204/314 (64.97%), Query Frame = 0


Query: 3   IGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPNV-LWLGTRNEDI 62
           IG+NYGL G+NLP  + VI LY    I  +RIF+PN  VL+ALRG  ++ + +G R++D+
Sbjct: 43  IGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQDL 102

Query: 63  QSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRNIMQAL--V 122
            + +A + A   W  +++ PY +D+NIA+ITVGNEVIPG      +   +  +MQ+L  +
Sbjct: 103 AALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGP-----IGPQVLPVMQSLTNL 162

Query: 123 VTGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLLNVYPYF 182
           V      I ++TVV M  +  SYPPS G FT  A   L  + K++  + +P+L+ +YPYF
Sbjct: 163 VKSRNLPISISTVVAMWNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTNSPILVKIYPYF 222

Query: 183 AYASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVRLEISET 242
           +YAS+P  I L+YA F +   VV DG+L Y N+FDA+ D+F  A+EK G  D+ + +SET
Sbjct: 223 SYASDPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSET 282

Query: 243 GWPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLKLAGVEQ 302
           GWP+ GN   T+ D A TYN+NF+ H+ASG GTPKRP    +G LF  FNE+ K  G EQ
Sbjct: 283 GWPSAGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFLFATFNENQKPVGTEQ 342

Query: 303 NFGFFYPN-MQPVY 313
           NFG + PN M+P+Y
Sbjct: 343 NFGLYNPNDMKPIY 351

[Close]

BLAST of MC11g0678 vs. ExPASy Swiss-Prot
Match: Q02439 (Putative glucan endo-1,3-beta-glucosidase GVI (Fragment) OS=Hordeum vulgare OX=4513 PE=3 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 5.9e-68
Identity = 144/313 (46.01%), Postives = 197/313 (62.94%), Query Frame = 0


Query: 2   GIGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPNVLWLGTRNEDI 61
           GIGVNYG+ G++LP  + V+ LY    I  VRIF P+  VL+ALR     + LGT N D+
Sbjct: 6   GIGVNYGMMGSDLPSPDKVVALYKANNITDVRIFHPDTNVLEALRNSGLGVVLGTLNSDL 65

Query: 62  QSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRNIMQALVVT 121
              A+D   A +WV+S+V P+   V+  YI  GNEVIPG++AA  V  A++N+  AL   
Sbjct: 66  APLASDASYAASWVHSYVQPFAGAVSFRYINAGNEVIPGESAA-LVLPAMKNLEAALQAA 125

Query: 122 GVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLLNVYPYFAY 181
           G+   + VTT +  SV+G SYPPS G F++AA   +  I   + SSG PLL+NVYPYFAY
Sbjct: 126 GL--SVPVTTAMATSVLGTSYPPSQGTFSEAALPTVGPIVSHLASSGTPLLVNVYPYFAY 185

Query: 182 ASNPQQIPLNYALFTSTTPV-VVDGNLNYYNLFDAMVDSFYAALEKIGAVD-VRLEISET 241
           +++P  + L+YAL +S+  V V D  + Y N+FDA++D+ YAA+EK G  + + L +SET
Sbjct: 186 SADPSSVRLDYALLSSSAAVAVTDNGVEYANMFDAILDAVYAAVEKAGGGESLELVVSET 245

Query: 242 GWPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLKLAGVEQ 301
           GWP+ G     S++NA  Y  N + HV    GTP+RP       +F MFNE+ K  GVEQ
Sbjct: 246 GWPSGGGGYGASVENAAAYINNLVRHV---GGTPRRPGKAVETYIFAMFNENQKPEGVEQ 305

Query: 302 NFGFFYPNMQPVY 313
           NFG F P+M  VY
Sbjct: 306 NFGMFQPDMSQVY 312

[Close]

BLAST of MC11g0678 vs. ExPASy Swiss-Prot
Match: A7PQW3 (Glucan endo-1,3-beta-glucosidase OS=Vitis vinifera OX=29760 GN=VIT_06s0061g00120 PE=1 SV=2)

HSP 1 Score: 258.8 bits (660), Expect = 7.7e-68
Identity = 143/314 (45.54%), Postives = 199/314 (63.38%), Query Frame = 0


Query: 3   IGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPNVLWLGTRNEDIQ 62
           IGV YG NGNNLP A+ VINLY   GI  +RI++PN   L ALRG    L L   N D+Q
Sbjct: 30  IGVCYGRNGNNLPSASQVINLYKSNGIGSMRIYDPNSDTLQALRGSDIELILDVPNTDLQ 89

Query: 63  SFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIP-GDAA--APFVANAIRNIMQALV 122
           S A+D  AA  WV ++VV Y ++V   YI VGNEV+P G  A  A +V  A++N+  A+ 
Sbjct: 90  SLASDASAAATWVQNNVVNYASEVKFRYIAVGNEVLPTGSNAQYAQYVLPAMKNVQSAIT 149

Query: 123 VTGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLLNVYPYF 182
             G++  IKV+T    +V+G SYPPS G+F+D  +  ++ I   +  +G+PLL N+YPYF
Sbjct: 150 SAGLQDQIKVSTATFSAVLGKSYPPSEGSFSDDVSSFINPIISFLAENGSPLLANIYPYF 209

Query: 183 AYASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVRLEISET 242
           +Y  + Q I L+YALFT++  VV DG+  Y NLFDA++D+ YAALEK G  ++++ +SE+
Sbjct: 210 SYTGDTQNIRLDYALFTASGVVVQDGSYQYQNLFDALLDALYAALEKAGGSNLKIVVSES 269

Query: 243 GWPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLKLA-GVE 302
           GWP++G    T +DNA TY KN +NHV    GTP++        LF MF+E+ K     E
Sbjct: 270 GWPSEGGTAAT-VDNARTYYKNLINHVK--GGTPRKSGA-IETYLFAMFDENQKTGLETE 329

Query: 303 QNFGFFYPNMQPVY 313
           ++FG F P  +  Y
Sbjct: 330 KHFGLFTPGQESKY 339

[Close]

BLAST of MC11g0678 vs. NCBI nr
Match: XP_022135630.1 (probable glucan endo-1,3-beta-glucosidase BG4 [Momordica charantia])

HSP 1 Score: 625 bits (1612), Expect = 1.54e-225
Identity = 312/312 (100.00%), Postives = 312/312 (100.00%), Query Frame = 0


Query: 1   GGIGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPNVLWLGTRNED 60
           GGIGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPNVLWLGTRNED
Sbjct: 18  GGIGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPNVLWLGTRNED 77

Query: 61  IQSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRNIMQALVV 120
           IQSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRNIMQALVV
Sbjct: 78  IQSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRNIMQALVV 137

Query: 121 TGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLLNVYPYFA 180
           TGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLLNVYPYFA
Sbjct: 138 TGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLLNVYPYFA 197

Query: 181 YASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVRLEISETG 240
           YASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVRLEISETG
Sbjct: 198 YASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVRLEISETG 257

Query: 241 WPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLKLAGVEQN 300
           WPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLKLAGVEQN
Sbjct: 258 WPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLKLAGVEQN 317

Query: 301 FGFFYPNMQPVY 312
           FGFFYPNMQPVY
Sbjct: 318 FGFFYPNMQPVY 329

[Close]

BLAST of MC11g0678 vs. NCBI nr
Match: XP_022152302.1 (probable glucan endo-1,3-beta-glucosidase BG4 [Momordica charantia])

HSP 1 Score: 560 bits (1442), Expect = 1.79e-199
Identity = 273/312 (87.50%), Postives = 289/312 (92.63%), Query Frame = 0


Query: 1   GGIGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPNVLWLGTRNED 60
           GGIGVNYGLNG+NLPQ +DVINLYGRCGIDIVRIFEPNH VLDAL GKPN LWLGTRNED
Sbjct: 28  GGIGVNYGLNGDNLPQPSDVINLYGRCGIDIVRIFEPNHGVLDALHGKPNALWLGTRNED 87

Query: 61  IQSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRNIMQALVV 120
           I SFA DQ+AANAWVN+HV+PYYADVNI YITVGNEV+P D A PFVANAIRNIMQALV 
Sbjct: 88  IHSFATDQMAANAWVNAHVLPYYADVNIEYITVGNEVVPADPATPFVANAIRNIMQALVT 147

Query: 121 TGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLLNVYPYFA 180
            G+K+DIKVTTVVHM  +GVSYPPS G FT AAAGPLS+IAKVVGSSG PLLLNVYPYFA
Sbjct: 148 AGIKKDIKVTTVVHMGAMGVSYPPSAGVFTVAAAGPLSDIAKVVGSSGTPLLLNVYPYFA 207

Query: 181 YASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVRLEISETG 240
           YAS+PQQIPLNYALFTSTTP VVDGNLNYYNLFDAMVDSFYAALEKI A +VRLEISETG
Sbjct: 208 YASSPQQIPLNYALFTSTTPGVVDGNLNYYNLFDAMVDSFYAALEKIDAANVRLEISETG 267

Query: 241 WPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLKLAGVEQN 300
           WPTKGN+PFTS++NAVTYNKNFMNHVASGAGTP+RP VKYNGILFEMFNEDLK AGVEQN
Sbjct: 268 WPTKGNEPFTSVENAVTYNKNFMNHVASGAGTPRRPNVKYNGILFEMFNEDLKAAGVEQN 327

Query: 301 FGFFYPNMQPVY 312
           FGFFYPNMQPVY
Sbjct: 328 FGFFYPNMQPVY 339

[Close]

BLAST of MC11g0678 vs. NCBI nr
Match: XP_022141986.1 (probable glucan endo-1,3-beta-glucosidase BG4 [Momordica charantia])

HSP 1 Score: 540 bits (1391), Expect = 1.01e-191
Identity = 267/312 (85.58%), Postives = 287/312 (91.99%), Query Frame = 0


Query: 1   GGIGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPNVLWLGTRNED 60
           GGIGVNYGLNG+NLPQ NDVI+LYGRCGIDIVRIFEPNH VLDA+RGKPN LWLGTRNED
Sbjct: 28  GGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALWLGTRNED 87

Query: 61  IQSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRNIMQALVV 120
           I SFA +Q AANAWVN++VVPYYADVNIAYITVGNEVIP D AAPFVANAIRNIMQAL +
Sbjct: 88  IPSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRNIMQALAI 147

Query: 121 TGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLLNVYPYFA 180
           TGVK+ IKVTTVV MS +GVSYPPS G FT  A GPLS IAKVVGSSGAPLLLNVYPYFA
Sbjct: 148 TGVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLLLNVYPYFA 207

Query: 181 YASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVRLEISETG 240
           YASNPQQI LNYALFTSTTP+V DGNL+YYNLFDAMVDSFYAALEKIGA +V LEISETG
Sbjct: 208 YASNPQQISLNYALFTSTTPIV-DGNLHYYNLFDAMVDSFYAALEKIGATNVTLEISETG 267

Query: 241 WPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLKLAGVEQN 300
           WPTKGN+PFTS++NAVTYNKNF+NHV+SGAGTP+RP VKY+GILFEMFNEDLK AG+EQN
Sbjct: 268 WPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKAAGIEQN 327

Query: 301 FGFFYPNMQPVY 312
           FGFFYPNM PVY
Sbjct: 328 FGFFYPNMHPVY 338

[Close]

BLAST of MC11g0678 vs. NCBI nr
Match: XP_022935981.1 (probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita moschata])

HSP 1 Score: 505 bits (1300), Expect = 7.84e-178
Identity = 245/313 (78.27%), Postives = 280/313 (89.46%), Query Frame = 0


Query: 1   GGIGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPN-VLWLGTRNE 60
           G +GVNYGLNG+NLP A+DV+ LYGRCGIDIVR+FEPNH+VLDAL+G+ N V+WLGTRNE
Sbjct: 28  GAVGVNYGLNGDNLPVASDVVELYGRCGIDIVRVFEPNHWVLDALKGRRNLVVWLGTRNE 87

Query: 61  DIQSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRNIMQALV 120
           DIQ FAADQLAAN+WVN+HVVPYY  VNIAYITVGNEV+PGDAAAPFVANAI+NIMQALV
Sbjct: 88  DIQGFAADQLAANSWVNAHVVPYYESVNIAYITVGNEVVPGDAAAPFVANAIKNIMQALV 147

Query: 121 VTGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLLNVYPYF 180
           + GVK DIKVTTVV M+ + VSYPPS GAF+  A GPL +I +V+G+SGAPLLLNVYPYF
Sbjct: 148 IAGVKSDIKVTTVVPMTPLEVSYPPSAGAFSATAIGPLKDITQVLGTSGAPLLLNVYPYF 207

Query: 181 AYASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVRLEISET 240
           AYASNPQQI L+YALFTSTTPVVVDGNL Y+NLFDAMVDSFY+ALEKIGA  VR++ISET
Sbjct: 208 AYASNPQQISLDYALFTSTTPVVVDGNLQYFNLFDAMVDSFYSALEKIGAERVRIDISET 267

Query: 241 GWPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLKLAGVEQ 300
           GWP+KGN   TS++NA TYN+NF+ HV SGAGTP+RP VKY+G+LFEMFNE+LK AGVEQ
Sbjct: 268 GWPSKGNDQLTSVENAATYNRNFVEHVRSGAGTPRRPNVKYDGVLFEMFNENLKTAGVEQ 327

Query: 301 NFGFFYPNMQPVY 312
           NFG FYPNM PVY
Sbjct: 328 NFGLFYPNMLPVY 340

[Close]

BLAST of MC11g0678 vs. NCBI nr
Match: KAG6591759.1 (Glucan endo-1,3-beta-glucosidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 503 bits (1296), Expect = 3.19e-177
Identity = 243/313 (77.64%), Postives = 278/313 (88.82%), Query Frame = 0


Query: 1   GGIGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPN-VLWLGTRNE 60
           G +GVNYGLNG+NLP A++V+ LYGRCGIDI+R++EPNH VLDAL+G+ N V+WLGTRNE
Sbjct: 28  GAVGVNYGLNGDNLPVASEVVELYGRCGIDILRVYEPNHSVLDALKGRRNLVVWLGTRNE 87

Query: 61  DIQSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRNIMQALV 120
           DIQ FAADQLAAN+WVN++VVPYY  VNIAY+TVGNEV+P DAAAPFVANAI+NIMQALV
Sbjct: 88  DIQGFAADQLAANSWVNANVVPYYKSVNIAYVTVGNEVVPDDAAAPFVANAIKNIMQALV 147

Query: 121 VTGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLLNVYPYF 180
           + GVK DIKVTTVV MSV+ VSYPPS GAF+  A GPL +I +V+G+SGAPLLLN YPYF
Sbjct: 148 IAGVKSDIKVTTVVPMSVLAVSYPPSAGAFSGTAIGPLKDITQVLGTSGAPLLLNAYPYF 207

Query: 181 AYASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVRLEISET 240
           AYASNPQQI L+YALF ST PVVVDGNL Y+NLFDAMVDSFY+ALEKIGA  VR++ISET
Sbjct: 208 AYASNPQQISLDYALFRSTAPVVVDGNLKYFNLFDAMVDSFYSALEKIGAEGVRIDISET 267

Query: 241 GWPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLKLAGVEQ 300
           GWP+KGN+PFTS+DNA TYNK F+ HV SGAGTPKRP VKY+G+LFEMFNEDLK AGVEQ
Sbjct: 268 GWPSKGNEPFTSVDNAATYNKKFVEHVRSGAGTPKRPNVKYDGVLFEMFNEDLKTAGVEQ 327

Query: 301 NFGFFYPNMQPVY 312
           NFG FYPNM PVY
Sbjct: 328 NFGMFYPNMHPVY 340

[Close]

BLAST of MC11g0678 vs. ExPASy TrEMBL
Match: A0A6J1C1J7 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111007535 PE=3 SV=1)

HSP 1 Score: 625 bits (1612), Expect = 7.46e-226
Identity = 312/312 (100.00%), Postives = 312/312 (100.00%), Query Frame = 0


Query: 1   GGIGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPNVLWLGTRNED 60
           GGIGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPNVLWLGTRNED
Sbjct: 18  GGIGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPNVLWLGTRNED 77

Query: 61  IQSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRNIMQALVV 120
           IQSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRNIMQALVV
Sbjct: 78  IQSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRNIMQALVV 137

Query: 121 TGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLLNVYPYFA 180
           TGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLLNVYPYFA
Sbjct: 138 TGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLLNVYPYFA 197

Query: 181 YASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVRLEISETG 240
           YASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVRLEISETG
Sbjct: 198 YASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVRLEISETG 257

Query: 241 WPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLKLAGVEQN 300
           WPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLKLAGVEQN
Sbjct: 258 WPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLKLAGVEQN 317

Query: 301 FGFFYPNMQPVY 312
           FGFFYPNMQPVY
Sbjct: 318 FGFFYPNMQPVY 329

[Close]

BLAST of MC11g0678 vs. ExPASy TrEMBL
Match: A0A6J1DFU0 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111020050 PE=3 SV=1)

HSP 1 Score: 560 bits (1442), Expect = 8.69e-200
Identity = 273/312 (87.50%), Postives = 289/312 (92.63%), Query Frame = 0


Query: 1   GGIGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPNVLWLGTRNED 60
           GGIGVNYGLNG+NLPQ +DVINLYGRCGIDIVRIFEPNH VLDAL GKPN LWLGTRNED
Sbjct: 28  GGIGVNYGLNGDNLPQPSDVINLYGRCGIDIVRIFEPNHGVLDALHGKPNALWLGTRNED 87

Query: 61  IQSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRNIMQALVV 120
           I SFA DQ+AANAWVN+HV+PYYADVNI YITVGNEV+P D A PFVANAIRNIMQALV 
Sbjct: 88  IHSFATDQMAANAWVNAHVLPYYADVNIEYITVGNEVVPADPATPFVANAIRNIMQALVT 147

Query: 121 TGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLLNVYPYFA 180
            G+K+DIKVTTVVHM  +GVSYPPS G FT AAAGPLS+IAKVVGSSG PLLLNVYPYFA
Sbjct: 148 AGIKKDIKVTTVVHMGAMGVSYPPSAGVFTVAAAGPLSDIAKVVGSSGTPLLLNVYPYFA 207

Query: 181 YASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVRLEISETG 240
           YAS+PQQIPLNYALFTSTTP VVDGNLNYYNLFDAMVDSFYAALEKI A +VRLEISETG
Sbjct: 208 YASSPQQIPLNYALFTSTTPGVVDGNLNYYNLFDAMVDSFYAALEKIDAANVRLEISETG 267

Query: 241 WPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLKLAGVEQN 300
           WPTKGN+PFTS++NAVTYNKNFMNHVASGAGTP+RP VKYNGILFEMFNEDLK AGVEQN
Sbjct: 268 WPTKGNEPFTSVENAVTYNKNFMNHVASGAGTPRRPNVKYNGILFEMFNEDLKAAGVEQN 327

Query: 301 FGFFYPNMQPVY 312
           FGFFYPNMQPVY
Sbjct: 328 FGFFYPNMQPVY 339

[Close]

BLAST of MC11g0678 vs. ExPASy TrEMBL
Match: A0A6J1CKU8 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111012224 PE=3 SV=1)

HSP 1 Score: 540 bits (1391), Expect = 4.91e-192
Identity = 267/312 (85.58%), Postives = 287/312 (91.99%), Query Frame = 0


Query: 1   GGIGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPNVLWLGTRNED 60
           GGIGVNYGLNG+NLPQ NDVI+LYGRCGIDIVRIFEPNH VLDA+RGKPN LWLGTRNED
Sbjct: 28  GGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALWLGTRNED 87

Query: 61  IQSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRNIMQALVV 120
           I SFA +Q AANAWVN++VVPYYADVNIAYITVGNEVIP D AAPFVANAIRNIMQAL +
Sbjct: 88  IPSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRNIMQALAI 147

Query: 121 TGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLLNVYPYFA 180
           TGVK+ IKVTTVV MS +GVSYPPS G FT  A GPLS IAKVVGSSGAPLLLNVYPYFA
Sbjct: 148 TGVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLLLNVYPYFA 207

Query: 181 YASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVRLEISETG 240
           YASNPQQI LNYALFTSTTP+V DGNL+YYNLFDAMVDSFYAALEKIGA +V LEISETG
Sbjct: 208 YASNPQQISLNYALFTSTTPIV-DGNLHYYNLFDAMVDSFYAALEKIGATNVTLEISETG 267

Query: 241 WPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLKLAGVEQN 300
           WPTKGN+PFTS++NAVTYNKNF+NHV+SGAGTP+RP VKY+GILFEMFNEDLK AG+EQN
Sbjct: 268 WPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKAAGIEQN 327

Query: 301 FGFFYPNMQPVY 312
           FGFFYPNM PVY
Sbjct: 328 FGFFYPNMHPVY 338

[Close]

BLAST of MC11g0678 vs. ExPASy TrEMBL
Match: A0A6J1F682 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111442719 PE=3 SV=1)

HSP 1 Score: 505 bits (1300), Expect = 3.80e-178
Identity = 245/313 (78.27%), Postives = 280/313 (89.46%), Query Frame = 0


Query: 1   GGIGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPN-VLWLGTRNE 60
           G +GVNYGLNG+NLP A+DV+ LYGRCGIDIVR+FEPNH+VLDAL+G+ N V+WLGTRNE
Sbjct: 28  GAVGVNYGLNGDNLPVASDVVELYGRCGIDIVRVFEPNHWVLDALKGRRNLVVWLGTRNE 87

Query: 61  DIQSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRNIMQALV 120
           DIQ FAADQLAAN+WVN+HVVPYY  VNIAYITVGNEV+PGDAAAPFVANAI+NIMQALV
Sbjct: 88  DIQGFAADQLAANSWVNAHVVPYYESVNIAYITVGNEVVPGDAAAPFVANAIKNIMQALV 147

Query: 121 VTGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLLNVYPYF 180
           + GVK DIKVTTVV M+ + VSYPPS GAF+  A GPL +I +V+G+SGAPLLLNVYPYF
Sbjct: 148 IAGVKSDIKVTTVVPMTPLEVSYPPSAGAFSATAIGPLKDITQVLGTSGAPLLLNVYPYF 207

Query: 181 AYASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVRLEISET 240
           AYASNPQQI L+YALFTSTTPVVVDGNL Y+NLFDAMVDSFY+ALEKIGA  VR++ISET
Sbjct: 208 AYASNPQQISLDYALFTSTTPVVVDGNLQYFNLFDAMVDSFYSALEKIGAERVRIDISET 267

Query: 241 GWPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLKLAGVEQ 300
           GWP+KGN   TS++NA TYN+NF+ HV SGAGTP+RP VKY+G+LFEMFNE+LK AGVEQ
Sbjct: 268 GWPSKGNDQLTSVENAATYNRNFVEHVRSGAGTPRRPNVKYDGVLFEMFNENLKTAGVEQ 327

Query: 301 NFGFFYPNMQPVY 312
           NFG FYPNM PVY
Sbjct: 328 NFGLFYPNMLPVY 340

[Close]

BLAST of MC11g0678 vs. ExPASy TrEMBL
Match: A0A6J1FC84 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111442718 PE=3 SV=1)

HSP 1 Score: 498 bits (1281), Expect = 2.96e-175
Identity = 242/313 (77.32%), Postives = 275/313 (87.86%), Query Frame = 0


Query: 1   GGIGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPN-VLWLGTRNE 60
           G +GVNYGLNG+NLP  ++V+ LYGRCGIDI+R++EPNH VLDAL+G+ N V+WLGTRNE
Sbjct: 28  GAVGVNYGLNGDNLPVPSEVVGLYGRCGIDILRVYEPNHSVLDALKGRRNLVVWLGTRNE 87

Query: 61  DIQSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRNIMQALV 120
           DIQ FAADQLAAN+WVN++VVPYY  VNIAYITVGNEV+P DAAAPFVANAI+NIMQALV
Sbjct: 88  DIQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVANAIKNIMQALV 147

Query: 121 VTGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLLNVYPYF 180
           + GVK DIKVTTVV MSV+ VSYPPS GAF+  A GPL +I +V+G+SGAPLLLN YPYF
Sbjct: 148 IAGVKSDIKVTTVVPMSVLAVSYPPSAGAFSATAIGPLKDITQVLGTSGAPLLLNAYPYF 207

Query: 181 AYASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVRLEISET 240
           AYASNPQQI L+YALF ST PVVVDGNL Y+NLFDAMVDSFY+ALEKIGA  VR++ISET
Sbjct: 208 AYASNPQQISLDYALFKSTAPVVVDGNLKYFNLFDAMVDSFYSALEKIGAEGVRIDISET 267

Query: 241 GWPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLKLAGVEQ 300
           GWP+KGN PFTS+DNA TYNK F+ HV SGAGTPKRP VKY+G+LFEMFNEDLK AGVEQ
Sbjct: 268 GWPSKGNDPFTSVDNAATYNKKFVEHVRSGAGTPKRPNVKYDGVLFEMFNEDLKTAGVEQ 327

Query: 301 NFGFFYPNMQPVY 312
           NFG FYPNM  VY
Sbjct: 328 NFGMFYPNMHSVY 340

[Close]

BLAST of MC11g0678 vs. TAIR 10
Match: AT5G20330.1 (beta-1,3-glucanase 4 )

HSP 1 Score: 284.3 bits (726), Expect = 1.2e-76
Identity = 149/314 (47.45%), Postives = 208/314 (66.24%), Query Frame = 0


Query: 3   IGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPNV-LWLGTRNEDI 62
           IG+NYGL G+NLP  ++VINLY   GI  +RIF+PN  VL+ALRG  ++ + +G +++D+
Sbjct: 34  IGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTVGVKDQDL 93

Query: 63  QSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRNIMQAL--V 122
            + AA + A   W  +++  Y ADVNI +ITVGNEVIPG      +   +  +MQ+L  +
Sbjct: 94  AALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGP-----IGPQVLPVMQSLTNL 153

Query: 123 VTGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLLNVYPYF 182
           V      I ++TVV MS +  SYPPS G FT  A   L  + K++  +  P+L+N+YPYF
Sbjct: 154 VKSRNLPISISTVVAMSNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNIYPYF 213

Query: 183 AYASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVRLEISET 242
           AYAS+P  I L+YA F + + VV DG+L Y N+FDA+ D+F  A+EK G  ++ + +SET
Sbjct: 214 AYASDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPMVVSET 273

Query: 243 GWPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLKLAGVEQ 302
           GWP+ GN  FT+   A TYN+NF+ H+ASG GTPKRP    NG LF  FNE+ K AG EQ
Sbjct: 274 GWPSAGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFNENQKPAGTEQ 333

Query: 303 NFGFFYP-NMQPVY 313
           NFG + P +M+P+Y
Sbjct: 334 NFGLYNPSDMKPIY 342

[Close]

BLAST of MC11g0678 vs. TAIR 10
Match: AT5G20340.1 (beta-1,3-glucanase 5 )

HSP 1 Score: 275.8 bits (704), Expect = 4.3e-74
Identity = 144/314 (45.86%), Postives = 204/314 (64.97%), Query Frame = 0


Query: 3   IGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPNV-LWLGTRNEDI 62
           IG+NYGL G+NLP  + VI LY    I  +RIF+PN  VL+ALRG  ++ + +G R++D+
Sbjct: 43  IGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQDL 102

Query: 63  QSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRNIMQAL--V 122
            + +A + A   W  +++ PY +D+NIA+ITVGNEVIPG      +   +  +MQ+L  +
Sbjct: 103 AALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGP-----IGPQVLPVMQSLTNL 162

Query: 123 VTGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLLNVYPYF 182
           V      I ++TVV M  +  SYPPS G FT  A   L  + K++  + +P+L+ +YPYF
Sbjct: 163 VKSRNLPISISTVVAMWNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTNSPILVKIYPYF 222

Query: 183 AYASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVRLEISET 242
           +YAS+P  I L+YA F +   VV DG+L Y N+FDA+ D+F  A+EK G  D+ + +SET
Sbjct: 223 SYASDPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSET 282

Query: 243 GWPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLKLAGVEQ 302
           GWP+ GN   T+ D A TYN+NF+ H+ASG GTPKRP    +G LF  FNE+ K  G EQ
Sbjct: 283 GWPSAGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFLFATFNENQKPVGTEQ 342

Query: 303 NFGFFYPN-MQPVY 313
           NFG + PN M+P+Y
Sbjct: 343 NFGLYNPNDMKPIY 351

[Close]

BLAST of MC11g0678 vs. TAIR 10
Match: AT1G77790.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 275.0 bits (702), Expect = 7.3e-74
Identity = 149/308 (48.38%), Postives = 196/308 (63.64%), Query Frame = 0


Query: 7   YGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPNVLWLGTRNEDIQSFAA 66
           YG NGNNLP   D + LY    ID +R++EP   +L+ALRG   ++  G RNEDIQS A 
Sbjct: 34  YGRNGNNLPTPADTVALYKSNNIDAIRMYEPFADMLEALRGSGLLVAFGPRNEDIQSLAH 93

Query: 67  DQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRNIMQALVVTGVKRD 126
           D  AA  +V++ + PY  DV I +IT+GNEV PG+  A FVA AI+N+  AL  +GV   
Sbjct: 94  DPAAATNFVSTWITPYQNDVAIKWITIGNEVFPGE-IAQFVAAAIKNVNVALTNSGV-TG 153

Query: 127 IKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLLNVYPYFAYASNPQ 186
           I VTTV+ M+ +  +YPPS   F       ++EI  ++  + +PL+ N+YPYFAYAS+P 
Sbjct: 154 ISVTTVLAMTALTNTYPPSAATFLPDLTEIMTEITSILSETNSPLMTNIYPYFAYASDPY 213

Query: 187 QIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVRLEISETGWPTKGN 246
            I L+YA F S TPVV+DG+L Y N+F+AMVD F AALEKI A +V + ++ETGWPT+GN
Sbjct: 214 HISLDYASFKSNTPVVIDGDLYYNNMFEAMVDGFNAALEKINAANVVVMVAETGWPTEGN 273

Query: 247 QPFTSIDNAVTYNKNF--MNHVASGAGTPKRPKVKYNGILFEMFNEDLKLAGVEQNFGFF 306
            P TS+DNA  YN         A    TP+R     +  LF MF E+ K   VEQ+FG F
Sbjct: 274 PPHTSVDNAKAYNMGIRTCGRSAERKRTPRRQNTPVDVFLFAMFKENQKDGPVEQSFGIF 333

Query: 307 YPNMQPVY 313
            P+M PVY
Sbjct: 334 APDMTPVY 339

[Close]

BLAST of MC11g0678 vs. TAIR 10
Match: AT1G77780.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 272.3 bits (695), Expect = 4.8e-73
Identity = 140/309 (45.31%), Postives = 198/309 (64.08%), Query Frame = 0


Query: 4   GVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPNVLWLGTRNEDIQS 63
           GV YG NG+NLP     ++LY +  +  +R++EP   ++ +L+G   ++ +G RNE I++
Sbjct: 25  GVCYGRNGDNLPSPAKTVSLYKKINVGGIRLYEPFPDLIVSLQGTGLLVAIGPRNEAIKT 84

Query: 64  FAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRNIMQALVVTGV 123
            A +   A  W  + + P Y +V   +ITVGNEVI G+    +V  A++NI  AL   G 
Sbjct: 85  LAEEYQFALNWDKTFIAP-YKNVAFNWITVGNEVIEGEIGR-YVPQAMKNIKAALTEIGN 144

Query: 124 KRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLLNVYPYFAYAS 183
            + I VTTV+  + +  SYPPS G F  A    ++EI  ++ S+ +PL++NVYPYFAYAS
Sbjct: 145 SK-IHVTTVISTAALANSYPPSAGVFKPAITELITEIVSILSSTDSPLMVNVYPYFAYAS 204

Query: 184 NPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVRLEISETGWPT 243
           +P  + L YA F ST+PVV DG   Y N+FDA +D+F  ALEKI    V++ ++ETGWPT
Sbjct: 205 DPSHVSLEYATFRSTSPVVTDGKYQYTNIFDATLDAFNVALEKINHGSVKVYVAETGWPT 264

Query: 244 KGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLKLAGVEQNFGF 303
           +GN P+TS++NA  YN+  +  + +G GTP+RP V      FEMFNEDLK   VEQ+FGF
Sbjct: 265 RGNDPYTSVENARAYNQGLLKKLTTGKGTPRRPNVPVITFFFEMFNEDLKQGAVEQSFGF 324

Query: 304 FYPNMQPVY 313
           F PNM PVY
Sbjct: 325 FDPNMAPVY 330

[Close]

BLAST of MC11g0678 vs. TAIR 10
Match: AT1G33220.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 270.0 bits (689), Expect = 2.4e-72
Identity = 142/313 (45.37%), Postives = 200/313 (63.90%), Query Frame = 0


Query: 3   IGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPNV-LWLGTRNEDI 62
           IG+NYGL G+N P  ++V+NLY   GI  +RIF+P   VL+ALRG  N+ + +G R++D+
Sbjct: 34  IGLNYGLLGDNFPTPSNVLNLYKSIGITKIRIFDPKTEVLNALRGHRNIEVTVGVRDQDL 93

Query: 63  QSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRNIMQAL--V 122
            + +A++ A   W  +++ PY ADVNIA+ITVGNEVIPG      +   +  +MQ+L  +
Sbjct: 94  GALSANEEAVKGWFATNIEPYLADVNIAFITVGNEVIPGP-----IGPQVLPVMQSLTIL 153

Query: 123 VTGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLLNVYPYF 182
           V  +   I ++TVV MS +  SYPPS G FT  A   L  + K++  +  P+L+N+YPYF
Sbjct: 154 VKSMNLPISISTVVAMSNLEQSYPPSAGEFTSQAREQLVPVLKLLSQTSTPILVNIYPYF 213

Query: 183 AYASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVRLEISET 242
            YAS+P  IPL+YA F +   VV DG L Y N+FDA+ D+F  A+EK G  D+ + +SET
Sbjct: 214 PYASDPTNIPLDYATFNTKATVVQDGPLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSET 273

Query: 243 GWPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLKLAGVEQ 302
           GWP+ GN   T+ D A TYN+NF+ H+ SG GTPKRP    +G LF  FNE+ K AG + 
Sbjct: 274 GWPSAGNGNLTTPDIAGTYNRNFVKHIVSGKGTPKRPNNGMDGFLFATFNENQKPAGTD- 332

Query: 303 NFGFFYPNMQPVY 313
                   M+P+Y
Sbjct: 334 --------MKPIY 332

The following BLAST results are available for this feature:

BLAST of MC11g0678 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22317	2.7e-81	49.35	Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2	[more]
O49352	1.7e-75	47.45	Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN...	[more]
O49353	6.1e-73	45.86	Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q02439	5.9e-68	46.01	Putative glucan endo-1,3-beta-glucosidase GVI (Fragment) OS=Hordeum vulgare OX=4...	[more]
A7PQW3	7.7e-68	45.54	Glucan endo-1,3-beta-glucosidase OS=Vitis vinifera OX=29760 GN=VIT_06s0061g00120...	[more]

BLAST of MC11g0678 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135630.1	1.54e-225	100.00	probable glucan endo-1,3-beta-glucosidase BG4 [Momordica charantia]	[more]
XP_022152302.1	1.79e-199	87.50	probable glucan endo-1,3-beta-glucosidase BG4 [Momordica charantia]	[more]
XP_022141986.1	1.01e-191	85.58	probable glucan endo-1,3-beta-glucosidase BG4 [Momordica charantia]	[more]
XP_022935981.1	7.84e-178	78.27	probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita moschata]	[more]
KAG6591759.1	3.19e-177	77.64	Glucan endo-1,3-beta-glucosidase, partial [Cucurbita argyrosperma subsp. sororia...	[more]

BLAST of MC11g0678 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C1J7	7.46e-226	100.00	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111007535 ...	[more]
A0A6J1DFU0	8.69e-200	87.50	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111020050 ...	[more]
A0A6J1CKU8	4.91e-192	85.58	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111012224 ...	[more]
A0A6J1F682	3.80e-178	78.27	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111442719 P...	[more]
A0A6J1FC84	2.96e-175	77.32	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111442718 P...	[more]

BLAST of MC11g0678 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20330.1	1.2e-76	47.45	beta-1,3-glucanase 4	[more]
AT5G20340.1	4.3e-74	45.86	beta-1,3-glucanase 5	[more]
AT1G77790.1	7.3e-74	48.38	Glycosyl hydrolase superfamily protein	[more]
AT1G77780.1	4.8e-73	45.31	Glycosyl hydrolase superfamily protein	[more]
AT1G33220.1	2.4e-72	45.37	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 3..312 e-value: 2.8E-111 score: 373.5
None	No IPR available	PANTHER	PTHR32227:SF248	GLYCOSYL HYDROLASE SUPERFAMILY PROTEIN	coord: 3..312
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 3..312 e-value: 8.6E-90 score: 301.5
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 3..312
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 3..312

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC11g0678.1	MC11g0678.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds