MC11g0553 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC11g0553
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionsignal peptidase complex-like protein DTM1
LocationMC11: 4317782 .. 4319162 (-)
RNA-Seq ExpressionMC11g0553
SyntenyMC11g0553
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACAGGTTGGGATTCAAACTCACAACCTCTCCGTTGCAGATATATGTCAATTACGGCTAAGTTGTTTGGCTAATTAAACCATTTATATTGTAATTTATATCATACCTATTTTATAGTATCTATTCAACCATGTTCGTGCTAGTAATATAATATATTTCGAGCACGGAAGAGTCTGGTACTGCAATTTAAGAAGGGATCGTCTTTACTCGGCCGGAATGGTGGGGAAGGTAACCCATAAAAAGAAAAAAAAAATGTAACGATTGAATATTAAACTAAGTTCCCAGAAGAAAATGAGGGAAAAAAAAAGTCATTAAATATTGGATGGCAAAGCATTTAAAGATGCAGATTACAGTTGAAACCGAGCGGAGGCTATTCAAAGGAGCTGTCAAATAAATGCAATGACTAAAAGCAATCTCCAATCGGCAAACCCTAAACCCCGGCCCACACGCTCTCTGCAATGGCTGCCACTTCCAATTATTCTCGTAATTTCTCTTCCTTTCTGGCTCTGCATCCATGGCGGAAGACGCGGCTCTCAAATCCTCCCTCGTTTTCTTGGCGGCGGTCGTCGTTGTCGTCGGAATCGCCACGCATTCTTTCACGAACATGGCCGTAACTTATCTGGTCGGCGTTTTCGCGATCGCCGGCGTTCTCTTGCCGGACTGGTGTTTCTTTGACCGCGATTTCTCCCGGTGGACTTCTCCGGTCACCGCAGAGGAGAGGGAATCAGATCGGATCTCGACCGGATCTCAGTTACGGAGGTCGGTTACCTTTAATTTCTTCCCTTCCTGATTCTTCTCTTGTTTGATTGATGCGAAAGGAAAAAATTGGACGAGGAGAGAAATTTGATTTCGATTATTTGCAGCCTTGCGAAATTGATTTGGTAATTGTTTTGTTTTCGCAGTTTTTATTTGGATTTTTTGGAATTTGGTTTCTGCAGGTTTAGGATTTATCCAATCAGACTGATTGTTTACACCATAGTTTACAGTTTTGCTCTGTACAAATGGTGGAAATTCATTTCCAGCTAATCCACTTCCCTTCTGCAGTTGGCTAGTTGCTATTCCTACAGATAATTTTCTTTTTCTTTTTCCTTTTTTCTCTTCAGTAAAATAAATCTTTGTGATTTTGGTATTGATTTATGTCACATGAAAAACCTAGTAAATAAATCAAAACTTGTATTAGTTGTTTGTAGTATATATTCTAATCATCGTTTTTTTTTATTAAATATGTATTTTTTATTTCTTGTAATTATTTTGTCCTTCTCGAGGATTATTATAATTGCATTATTAGATTATACTTCAGAGGATACTTGGATGAACAAAATCTTAAGAACGCCCTTGTCAGTTTCGCGTTCTATTAATTGCATTATTCGATATGTTTGAG

mRNA sequence

AAACAGGTTGGGATTCAAACTCACAACCTCTCCGTTGCAGATATATGTCAATTACGGCTAAGTTGTTTGGCTAATTAAACCATTTATATTGTAATTTATATCATACCTATTTTATAGTATCTATTCAACCATGTTCGTGCTAGTAATATAATATATTTCGAGCACGGAAGAGTCTGGTACTGCAATTTAAGAAGGGATCGTCTTTACTCGGCCGGAATGGTGGGGAAGGTAACCCATAAAAAGAAAAAAAAAATGTAACGATTGAATATTAAACTAAGTTCCCAGAAGAAAATGAGGGAAAAAAAAAGTCATTAAATATTGGATGGCAAAGCATTTAAAGATGCAGATTACAGTTGAAACCGAGCGGAGGCTATTCAAAGGAGCTGTCAAATAAATGCAATGACTAAAAGCAATCTCCAATCGGCAAACCCTAAACCCCGGCCCACACGCTCTCTGCAATGGCTGCCACTTCCAATTATTCTCGTAATTTCTCTTCCTTTCTGGCTCTGCATCCATGGCGGAAGACGCGGCTCTCAAATCCTCCCTCGTTTTCTTGGCGGCGGTCGTCGTTGTCGTCGGAATCGCCACGCATTCTTTCACGAACATGGCCGTAACTTATCTGGTCGGCGTTTTCGCGATCGCCGGCGTTCTCTTGCCGGACTGGTGTTTCTTTGACCGCGATTTCTCCCGGTGGACTTCTCCGGTCACCGCAGAGGAGAGGGAATCAGATCGGATCTCGACCGGATCTCAGTTACGGAGGTTTAGGATTTATCCAATCAGACTGATTGTTTACACCATAGTTTACAGTTTTGCTCTGTACAAATGGTGGAAATTCATTTCCAGCTAATCCACTTCCCTTCTGCAGTTGGCTAGTTGCTATTCCTACAGATAATTTTCTTTTTCTTTTTCCTTTTTTCTCTTCAGTAAAATAAATCTTTGTGATTTTGGTATTGATTTATGTCACATGAAAAACCTAGTAAATAAATCAAAACTTGTATTAGTTGTTTGTAGTATATATTCTAATCATCGTTTTTTTTTATTAAATATGTATTTTTTATTTCTTGTAATTATTTTGTCCTTCTCGAGGATTATTATAATTGCATTATTAGATTATACTTCAGAGGATACTTGGATGAACAAAATCTTAAGAACGCCCTTGTCAGTTTCGCGTTCTATTAATTGCATTATTCGATATGTTTGAG

Coding sequence (CDS)

ATGGCGGAAGACGCGGCTCTCAAATCCTCCCTCGTTTTCTTGGCGGCGGTCGTCGTTGTCGTCGGAATCGCCACGCATTCTTTCACGAACATGGCCGTAACTTATCTGGTCGGCGTTTTCGCGATCGCCGGCGTTCTCTTGCCGGACTGGTGTTTCTTTGACCGCGATTTCTCCCGGTGGACTTCTCCGGTCACCGCAGAGGAGAGGGAATCAGATCGGATCTCGACCGGATCTCAGTTACGGAGGTTTAGGATTTATCCAATCAGACTGATTGTTTACACCATAGTTTACAGTTTTGCTCTGTACAAATGGTGGAAATTCATTTCCAGCTAA

Protein sequence

MAEDAALKSSLVFLAAVVVVVGIATHSFTNMAVTYLVGVFAIAGVLLPDWCFFDRDFSRWTSPVTAEERESDRISTGSQLRRFRIYPIRLIVYTIVYSFALYKWWKFISS
Homology
[Close]
BLAST of MC11g0553 vs. ExPASy Swiss-Prot
Match: Q7XI45 (Signal peptidase complex-like protein DTM1 OS=Oryza sativa subsp. japonica OX=39947 GN=DTM1 PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 8.5e-22
Identity = 53/112 (47.32%), Postives = 72/112 (64.29%), Query Frame = 0 

Query: 1   MAEDAALKSSLVFLAAVVVVVGIATHSFTNMAVTYLVGVFAIAGVLLPDWCFFDRDFSRW 60
           M  D  L+ SLV LAA VVV G+ T S    A TY  G+ AIAGVLLPDW FFDRD+S+W
Sbjct: 1   MGRDEMLRRSLVALAAAVVVTGVVTASVRKAAATYGFGILAIAGVLLPDWEFFDRDYSQW 60

Query: 61  TSPVTAEERESDRISTGSQ--LRRFRIYPIRLIVYTIVYSFALYKWWKFISS 111
            +P+ A  R +   +   +  + +F+ YP+R+ + T +Y F LYKWW ++SS
Sbjct: 61  LTPMPASRRTAAEAAADREHDVWKFKPYPLRMAMLTTIYGFGLYKWWMYVSS 112

[Close]
BLAST of MC11g0553 vs. NCBI nr
Match: XP_022152589.1 (signal peptidase complex-like protein DTM1 [Momordica charantia])

HSP 1 Score: 214 bits (545), Expect = 6.78e-70
Identity = 110/110 (100.00%), Postives = 110/110 (100.00%), Query Frame = 0 

Query: 1   MAEDAALKSSLVFLAAVVVVVGIATHSFTNMAVTYLVGVFAIAGVLLPDWCFFDRDFSRW 60
           MAEDAALKSSLVFLAAVVVVVGIATHSFTNMAVTYLVGVFAIAGVLLPDWCFFDRDFSRW
Sbjct: 1   MAEDAALKSSLVFLAAVVVVVGIATHSFTNMAVTYLVGVFAIAGVLLPDWCFFDRDFSRW 60

Query: 61  TSPVTAEERESDRISTGSQLRRFRIYPIRLIVYTIVYSFALYKWWKFISS 110
           TSPVTAEERESDRISTGSQLRRFRIYPIRLIVYTIVYSFALYKWWKFISS
Sbjct: 61  TSPVTAEERESDRISTGSQLRRFRIYPIRLIVYTIVYSFALYKWWKFISS 110

[Close]
BLAST of MC11g0553 vs. NCBI nr
Match: XP_023524429.1 (signal peptidase complex-like protein DTM1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 182 bits (461), Expect = 4.40e-57
Identity = 93/110 (84.55%), Postives = 99/110 (90.00%), Query Frame = 0 

Query: 1   MAEDAALKSSLVFLAAVVVVVGIATHSFTNMAVTYLVGVFAIAGVLLPDWCFFDRDFSRW 60
           MAEDAALKSSLVFLAAVVV+VGI+THSF  MAVTYLVGVFAIAG+LLPDWCFFDRDFSRW
Sbjct: 1   MAEDAALKSSLVFLAAVVVLVGISTHSFKKMAVTYLVGVFAIAGILLPDWCFFDRDFSRW 60

Query: 61  TSPVTAEERESDRISTGSQLRRFRIYPIRLIVYTIVYSFALYKWWKFISS 110
            SPVT EERES RIST S+  RFRIYPIRL VY IVYS ALYKWWKF+S+
Sbjct: 61  ISPVTEEERESYRISTASRNPRFRIYPIRLAVYAIVYSVALYKWWKFVST 110

[Close]
BLAST of MC11g0553 vs. NCBI nr
Match: KAG7037595.1 (Signal peptidase complex-like protein DTM1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 180 bits (457), Expect = 1.79e-56
Identity = 92/110 (83.64%), Postives = 98/110 (89.09%), Query Frame = 0 

Query: 1   MAEDAALKSSLVFLAAVVVVVGIATHSFTNMAVTYLVGVFAIAGVLLPDWCFFDRDFSRW 60
           MAEDAALKSSLVFLAA VV+VGI+THSF  MAVTYLVGVFAIAG+LLPDWCFFDRDFSRW
Sbjct: 1   MAEDAALKSSLVFLAAAVVLVGISTHSFKKMAVTYLVGVFAIAGILLPDWCFFDRDFSRW 60

Query: 61  TSPVTAEERESDRISTGSQLRRFRIYPIRLIVYTIVYSFALYKWWKFISS 110
            SPVT EERES RIST S+  RFRIYPIRL VY IVYS ALYKWWKF+S+
Sbjct: 61  ISPVTEEERESYRISTASRNPRFRIYPIRLAVYAIVYSVALYKWWKFVST 110

[Close]
BLAST of MC11g0553 vs. NCBI nr
Match: XP_022940831.1 (signal peptidase complex-like protein DTM1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 180 bits (456), Expect = 2.55e-56
Identity = 91/110 (82.73%), Postives = 99/110 (90.00%), Query Frame = 0 

Query: 1   MAEDAALKSSLVFLAAVVVVVGIATHSFTNMAVTYLVGVFAIAGVLLPDWCFFDRDFSRW 60
           MAEDAALKSSLVFLAA VV+VGI+THSF  MAVTYLVGVFAIAG+LLPDWCFFDR+FSRW
Sbjct: 1   MAEDAALKSSLVFLAAAVVLVGISTHSFKKMAVTYLVGVFAIAGILLPDWCFFDRNFSRW 60

Query: 61  TSPVTAEERESDRISTGSQLRRFRIYPIRLIVYTIVYSFALYKWWKFISS 110
            SPVT EERES RIST S+  RFRIYPIRL+VY IVYS ALYKWWKF+S+
Sbjct: 61  ISPVTEEERESYRISTASRNPRFRIYPIRLVVYAIVYSVALYKWWKFVST 110

[Close]
BLAST of MC11g0553 vs. NCBI nr
Match: KAG6608241.1 (Signal peptidase complex-like protein DTM1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 178 bits (452), Expect = 1.04e-55
Identity = 91/110 (82.73%), Postives = 98/110 (89.09%), Query Frame = 0 

Query: 1   MAEDAALKSSLVFLAAVVVVVGIATHSFTNMAVTYLVGVFAIAGVLLPDWCFFDRDFSRW 60
           MAEDAALKSSLVFLAA VV+VGI+THSF  MAVTYLVGVFAIAG+LLPDWCFFDR+FSRW
Sbjct: 1   MAEDAALKSSLVFLAAAVVLVGISTHSFKKMAVTYLVGVFAIAGILLPDWCFFDRNFSRW 60

Query: 61  TSPVTAEERESDRISTGSQLRRFRIYPIRLIVYTIVYSFALYKWWKFISS 110
            SPVT EERES RIST S+  RFRIYPIRL VY IVYS ALYKWWKF+S+
Sbjct: 61  ISPVTEEERESYRISTASRNPRFRIYPIRLAVYAIVYSVALYKWWKFVST 110

[Close]
BLAST of MC11g0553 vs. ExPASy TrEMBL
Match: A0A6J1DGG6 (signal peptidase complex-like protein DTM1 OS=Momordica charantia OX=3673 GN=LOC111020250 PE=3 SV=1)

HSP 1 Score: 214 bits (545), Expect = 3.28e-70
Identity = 110/110 (100.00%), Postives = 110/110 (100.00%), Query Frame = 0 

Query: 1   MAEDAALKSSLVFLAAVVVVVGIATHSFTNMAVTYLVGVFAIAGVLLPDWCFFDRDFSRW 60
           MAEDAALKSSLVFLAAVVVVVGIATHSFTNMAVTYLVGVFAIAGVLLPDWCFFDRDFSRW
Sbjct: 1   MAEDAALKSSLVFLAAVVVVVGIATHSFTNMAVTYLVGVFAIAGVLLPDWCFFDRDFSRW 60

Query: 61  TSPVTAEERESDRISTGSQLRRFRIYPIRLIVYTIVYSFALYKWWKFISS 110
           TSPVTAEERESDRISTGSQLRRFRIYPIRLIVYTIVYSFALYKWWKFISS
Sbjct: 61  TSPVTAEERESDRISTGSQLRRFRIYPIRLIVYTIVYSFALYKWWKFISS 110

[Close]
BLAST of MC11g0553 vs. ExPASy TrEMBL
Match: A0A6J1FJI2 (signal peptidase complex-like protein DTM1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446307 PE=3 SV=1)

HSP 1 Score: 180 bits (456), Expect = 1.23e-56
Identity = 91/110 (82.73%), Postives = 99/110 (90.00%), Query Frame = 0 

Query: 1   MAEDAALKSSLVFLAAVVVVVGIATHSFTNMAVTYLVGVFAIAGVLLPDWCFFDRDFSRW 60
           MAEDAALKSSLVFLAA VV+VGI+THSF  MAVTYLVGVFAIAG+LLPDWCFFDR+FSRW
Sbjct: 1   MAEDAALKSSLVFLAAAVVLVGISTHSFKKMAVTYLVGVFAIAGILLPDWCFFDRNFSRW 60

Query: 61  TSPVTAEERESDRISTGSQLRRFRIYPIRLIVYTIVYSFALYKWWKFISS 110
            SPVT EERES RIST S+  RFRIYPIRL+VY IVYS ALYKWWKF+S+
Sbjct: 61  ISPVTEEERESYRISTASRNPRFRIYPIRLVVYAIVYSVALYKWWKFVST 110

[Close]
BLAST of MC11g0553 vs. ExPASy TrEMBL
Match: A0A6J1J358 (signal peptidase complex-like protein DTM1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111480862 PE=3 SV=1)

HSP 1 Score: 178 bits (451), Expect = 7.13e-56
Identity = 91/110 (82.73%), Postives = 97/110 (88.18%), Query Frame = 0 

Query: 1   MAEDAALKSSLVFLAAVVVVVGIATHSFTNMAVTYLVGVFAIAGVLLPDWCFFDRDFSRW 60
           MAEDAALKSSLVFLAA VV+VGI+THSF  MAVTYLVGVFAIAG+LLPDWCFFDRDFSRW
Sbjct: 1   MAEDAALKSSLVFLAATVVLVGISTHSFKKMAVTYLVGVFAIAGILLPDWCFFDRDFSRW 60

Query: 61  TSPVTAEERESDRISTGSQLRRFRIYPIRLIVYTIVYSFALYKWWKFISS 110
            SPVT EERES RIST S+  RFRIYPIRL VY  VYS ALYKWWKF+S+
Sbjct: 61  ISPVTEEERESYRISTASRNPRFRIYPIRLAVYATVYSAALYKWWKFVST 110

[Close]
BLAST of MC11g0553 vs. ExPASy TrEMBL
Match: A0A5A7TAS9 (Signal peptidase complex-like protein DTM1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G006230 PE=3 SV=1)

HSP 1 Score: 171 bits (432), Expect = 5.62e-53
Identity = 85/110 (77.27%), Postives = 96/110 (87.27%), Query Frame = 0 

Query: 1   MAEDAALKSSLVFLAAVVVVVGIATHSFTNMAVTYLVGVFAIAGVLLPDWCFFDRDFSRW 60
           MA DAAL SSLV LA  +V+VGI T+SF  MAVTY VGVFAIAGVLLPDWCFFDRDFSRW
Sbjct: 1   MANDAALNSSLVILALAIVLVGITTYSFKKMAVTYFVGVFAIAGVLLPDWCFFDRDFSRW 60

Query: 61  TSPVTAEERESDRISTGSQLRRFRIYPIRLIVYTIVYSFALYKWWKFISS 110
           TSPVT EERES R +TGSQL+RFRIYP+R+IVY IVYS ALYKWW++++S
Sbjct: 61  TSPVTEEERESYRNATGSQLQRFRIYPMRVIVYGIVYSIALYKWWQYVTS 110

[Close]
BLAST of MC11g0553 vs. ExPASy TrEMBL
Match: A0A1S3CQR6 (uncharacterized protein LOC103503709 OS=Cucumis melo OX=3656 GN=LOC103503709 PE=3 SV=1)

HSP 1 Score: 171 bits (432), Expect = 5.62e-53
Identity = 85/110 (77.27%), Postives = 96/110 (87.27%), Query Frame = 0 

Query: 1   MAEDAALKSSLVFLAAVVVVVGIATHSFTNMAVTYLVGVFAIAGVLLPDWCFFDRDFSRW 60
           MA DAAL SSLV LA  +V+VGI T+SF  MAVTY VGVFAIAGVLLPDWCFFDRDFSRW
Sbjct: 1   MANDAALNSSLVILALAIVLVGITTYSFKKMAVTYFVGVFAIAGVLLPDWCFFDRDFSRW 60

Query: 61  TSPVTAEERESDRISTGSQLRRFRIYPIRLIVYTIVYSFALYKWWKFISS 110
           TSPVT EERES R +TGSQL+RFRIYP+R+IVY IVYS ALYKWW++++S
Sbjct: 61  TSPVTEEERESYRNATGSQLQRFRIYPMRVIVYGIVYSIALYKWWQYVTS 110

[Close]
BLAST of MC11g0553 vs. TAIR 10
Match: AT1G34640.1 (peptidases )

HSP 1 Score: 116.3 bits (290), Expect = 1.5e-26
Identity = 59/110 (53.64%), Postives = 78/110 (70.91%), Query Frame = 0 

Query: 1   MAEDAALKSSLVFLAAVVVVVGIATHSFTNMAVTYLVGVFAIAGVLLPDWCFFDRDFSRW 60
           M  +AAL+SS+V LA V+VVV + T S     VTY VG+  I G+LLPDW FFDR FSRW
Sbjct: 1   MGNEAALRSSMVGLAVVMVVVWLWTQSLKKTVVTYAVGISLIGGILLPDWDFFDRSFSRW 60

Query: 61  TSPVTAEERESDRISTGSQLRRFRIYPIRLIVYTIVYSFALYKWWKFISS 111
             PVTAEER S  ++  S   RFR+YP+R+++Y  VY +A+Y+WW F+S+
Sbjct: 61  GYPVTAEER-SAALARKSHPSRFRVYPMRMVLYGTVYGYAVYRWWMFVSN 109

The following BLAST results are available for this feature:
BLAST of MC11g0553 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q7XI458.5e-2247.32Signal peptidase complex-like protein DTM1 OS=Oryza sativa subsp. japonica OX=39... [more]
BLAST of MC11g0553 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022152589.16.78e-70100.00signal peptidase complex-like protein DTM1 [Momordica charantia][more]
XP_023524429.14.40e-5784.55signal peptidase complex-like protein DTM1 isoform X1 [Cucurbita pepo subsp. pep... [more]
KAG7037595.11.79e-5683.64Signal peptidase complex-like protein DTM1 [Cucurbita argyrosperma subsp. argyro... [more]
XP_022940831.12.55e-5682.73signal peptidase complex-like protein DTM1 isoform X1 [Cucurbita moschata][more]
KAG6608241.11.04e-5582.73Signal peptidase complex-like protein DTM1, partial [Cucurbita argyrosperma subs... [more]
BLAST of MC11g0553 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1DGG63.28e-70100.00signal peptidase complex-like protein DTM1 OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1FJI21.23e-5682.73signal peptidase complex-like protein DTM1 isoform X1 OS=Cucurbita moschata OX=3... [more]
A0A6J1J3587.13e-5682.73signal peptidase complex-like protein DTM1 isoform X1 OS=Cucurbita maxima OX=366... [more]
A0A5A7TAS95.62e-5377.27Signal peptidase complex-like protein DTM1 OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3CQR65.62e-5377.27uncharacterized protein LOC103503709 OS=Cucumis melo OX=3656 GN=LOC103503709 PE=... [more]
BLAST of MC11g0553 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G34640.11.5e-2653.64peptidases [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009542Signal peptidase complex subunit Spc1/SPCS1PFAMPF06645SPC12coord: 8..63
e-value: 3.1E-8
score: 33.6
IPR039955Signal peptidase complex-like protein DTM1PANTHERPTHR38354SIGNAL PEPTIDASE COMPLEX-LIKE PROTEIN DTM1coord: 1..110

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC11g0553.1MC11g0553.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0048658 anther wall tapetum development
biological_process GO:0006465 signal peptide processing
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005787 signal peptidase complex
cellular_component GO:0005789 endoplasmic reticulum membrane