MC11g0454 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC11g0454
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionENTH domain-containing protein
LocationMC11: 3551892 .. 3554077 (+)
RNA-Seq ExpressionMC11g0454
SyntenyMC11g0454
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGCCTAGTACGATCCGGAAAGCGATCGGAGCCGTGAAGGACCAGACGAGTATTGGAATTGCGAAGGTTGCGAGCAATATGGCGCCGGACCTTGAGGTAGCGATCGTGAAGGCTACGAGCCACGATGATGATCCAGCCAGCGAGAAGTACATCAGGGAGATTTTGAATCTGACATCGTATTCGCGTGGTTATGTGAGTGCGTGTGTGTCGGCGATTTCGAAGCGTCTGGCCAAGACGAGGGACTGGATAGTGGCGCTCAAGGCACTCATACTTGTGCACAGGTTGTTGAATGAAGGAGACCCGGTGTTTCAGGAGGAAATCTTGTATGCCACTAGAAGGGGCACGAGGCTATTGAATATGTCCGACTTTAAGGATGAAGCTCATTCGAGCTCGTGGGATCACTCGGCTTTCGTTCGAACTTATGCATTCTACTTGGACCAACGGCTGGAATTGATGTTGTTTGAGAAAAAGGGTGGTAGTACAAGGGGAAATTCCCGCGGAGATGATAGATTTGAAGGAAGAGATGACTTCAGATCTCCTCCGCCGAGGCCATATGATAATGGTTATGGTGAGTACAGGGGCGAGAGAGAGTATGGAAATTATGGTGAGATGAGGAGGTCGAGATCTTACGGGGATGTGGGGGAATCTGTGGGGAGGGAGGGGCAGGGGCAGGGGCGAAATAACAGAGGACCTGTGACCCCGTTGAGAGAAATGGTGACCGAGAGAATTTTCGGGAAGATGGGACATTTGCAGAGACTGTTGGACAGATTTTTGTCGTGCCGACCGACGGGTTTGGCGAAGAATAGTAGGATGATTTTGTATGCTTTGTACCCTCTAGTGAGGGAGAGTTTTCAGTTGTATGCAGATATTTGCGAGGTTTTGGCTGTTTTGCTTGACAAATTCTTTGATATGGAGTATTCTGACTGTATGAAGGCATTTGATGCGTATGGTAGTGCGGCCAAGCAGATTGACGAGCTGATTTCATTGTATAATTGGTGTAAAGATACAGGCGTTGCTAGGTCCTCTGAGTATCCAGAGGTGCAGAGAATTACCAGCAAGCTATTGGAGACATTGGAGGAGTTCCTTAGGGAAAGAGGGAAGAAGCCAAAGAGTCCGGAGAGGGAGCCTCCTCCGCCCGTGGCTCAAGAGGAAGAGCCAGTGCCTGATATGAATGAAATAAAAGCTCTGCCTCCACCTGAAAATTATACTCCTCCACCTCCGCCCGAGCCCGAGCCTGCACCCAAACCTCAACAACAAGTCTCAGAAGATTTGGTGAATCTGAGAGATGATGCAGTCAGTGCAGATGACCAAGGTAACAAACTAGCCCTGGCTTTGTTTGCTGGCCCAGCAGCTAATGGCGCGAACGGATCCTGGGAAGCTTTCCCTTCAGATGGACAGCCCGAAGTAACCTCTGCCTGGCAGACCCCGGCTGCTGAACCTGGAAAAGCCGATTGGGAGTTGGCTTTGGTGGAGACAGCGAGCAATTTATCAAGGCAGAAAGCAGCACTTGGTGGCGGTCTCGACCCGTTATTGCTAAATGGCATGTATGATCAAGGAATGGTCAGGCAGCACACGAGCACTGCCCAACTGAGTGGTGGAAGCGCTAGCAGTGTAGCATTGCCCGGCCCTGGAAACAGCAAAACTCCTGTACTGGCTCTTCCAGCTCCAGATGGAACTGTTCAGGCAGTGAATCAGGATCCTTTCGCCGCATCGTTAACCATTCCACCGCCTTCATACGTGCAAATGGTGGAGATGGAGAAGAAACAGCAACTGCTGGTGCAGGAGCAGCAGGTATGGCAGCAGTACTCAAGAGATGGGATGCAGGGGCAGAGCAGTTTGAACAAAATCAGTAACCCCCCAGGTTACTACACAGCGGGAATGATGGCTCCAATGCCGTACGGGATGCCTCCAGCAAACGGAATGGGCGGGTATTACTACATTCCTCAATGATATCTACACTTACCTTTTACATCATATATACTGTGTCCATGCTGTATTAGTAGTTTTATTTCTTTGTCTAAGTGATATAATTTTTGTTTCCTCTGCTATTTGTGCGAGAAAAATGGTGTGGGAACTTTTGTTTGACTCTGGACAGCTGATATTGTTTTCTGGGTTTTGATAATGGAAGACTCATACTTCCTACTCCAGTTCAAGTATCCGCACATTCCTTCTTGAAAATGCAC

mRNA sequence

ATGGCGCCTAGTACGATCCGGAAAGCGATCGGAGCCGTGAAGGACCAGACGAGTATTGGAATTGCGAAGGTTGCGAGCAATATGGCGCCGGACCTTGAGGTAGCGATCGTGAAGGCTACGAGCCACGATGATGATCCAGCCAGCGAGAAGTACATCAGGGAGATTTTGAATCTGACATCGTATTCGCGTGGTTATGTGAGTGCGTGTGTGTCGGCGATTTCGAAGCGTCTGGCCAAGACGAGGGACTGGATAGTGGCGCTCAAGGCACTCATACTTGTGCACAGGTTGTTGAATGAAGGAGACCCGGTGTTTCAGGAGGAAATCTTGTATGCCACTAGAAGGGGCACGAGGCTATTGAATATGTCCGACTTTAAGGATGAAGCTCATTCGAGCTCGTGGGATCACTCGGCTTTCGTTCGAACTTATGCATTCTACTTGGACCAACGGCTGGAATTGATGTTGTTTGAGAAAAAGGGTGGTAGTACAAGGGGAAATTCCCGCGGAGATGATAGATTTGAAGGAAGAGATGACTTCAGATCTCCTCCGCCGAGGCCATATGATAATGGTTATGGTGAGTACAGGGGCGAGAGAGAGTATGGAAATTATGGTGAGATGAGGAGGTCGAGATCTTACGGGGATGTGGGGGAATCTGTGGGGAGGGAGGGGCAGGGGCAGGGGCGAAATAACAGAGGACCTGTGACCCCGTTGAGAGAAATGGTGACCGAGAGAATTTTCGGGAAGATGGGACATTTGCAGAGACTGTTGGACAGATTTTTGTCGTGCCGACCGACGGGTTTGGCGAAGAATAGTAGGATGATTTTGTATGCTTTGTACCCTCTAGTGAGGGAGAGTTTTCAGTTGTATGCAGATATTTGCGAGGTTTTGGCTGTTTTGCTTGACAAATTCTTTGATATGGAGTATTCTGACTGTATGAAGGCATTTGATGCGTATGGTAGTGCGGCCAAGCAGATTGACGAGCTGATTTCATTGTATAATTGGTGTAAAGATACAGGCGTTGCTAGGTCCTCTGAGTATCCAGAGGTGCAGAGAATTACCAGCAAGCTATTGGAGACATTGGAGGAGTTCCTTAGGGAAAGAGGGAAGAAGCCAAAGAGTCCGGAGAGGGAGCCTCCTCCGCCCGTGGCTCAAGAGGAAGAGCCAGTGCCTGATATGAATGAAATAAAAGCTCTGCCTCCACCTGAAAATTATACTCCTCCACCTCCGCCCGAGCCCGAGCCTGCACCCAAACCTCAACAACAAGTCTCAGAAGATTTGGTGAATCTGAGAGATGATGCAGTCAGTGCAGATGACCAAGGTAACAAACTAGCCCTGGCTTTGTTTGCTGGCCCAGCAGCTAATGGCGCGAACGGATCCTGGGAAGCTTTCCCTTCAGATGGACAGCCCGAAGTAACCTCTGCCTGGCAGACCCCGGCTGCTGAACCTGGAAAAGCCGATTGGGAGTTGGCTTTGGTGGAGACAGCGAGCAATTTATCAAGGCAGAAAGCAGCACTTGGTGGCGGTCTCGACCCGTTATTGCTAAATGGCATGTATGATCAAGGAATGGTCAGGCAGCACACGAGCACTGCCCAACTGAGTGGTGGAAGCGCTAGCAGTGTAGCATTGCCCGGCCCTGGAAACAGCAAAACTCCTGTACTGGCTCTTCCAGCTCCAGATGGAACTGTTCAGGCAGTGAATCAGGATCCTTTCGCCGCATCGTTAACCATTCCACCGCCTTCATACGTGCAAATGGTGGAGATGGAGAAGAAACAGCAACTGCTGGTGCAGGAGCAGCAGGTATGGCAGCAGTACTCAAGAGATGGGATGCAGGGGCAGAGCAGTTTGAACAAAATCAGTAACCCCCCAGGTTACTACACAGCGGGAATGATGGCTCCAATGCCGTACGGGATGCCTCCAGCAAACGGAATGGGCGGGTATTACTACATTCCTCAATGATATCTACACTTACCTTTTACATCATATATACTGTGTCCATGCTGTATTAGTAGTTTTATTTCTTTGTCTAAGTGATATAATTTTTGTTTCCTCTGCTATTTGTGCGAGAAAAATGGTGTGGGAACTTTTGTTTGACTCTGGACAGCTGATATTGTTTTCTGGGTTTTGATAATGGAAGACTCATACTTCCTACTCCAGTTCAAGTATCCGCACATTCCTTCTTGAAAATGCAC

Coding sequence (CDS)

ATGGCGCCTAGTACGATCCGGAAAGCGATCGGAGCCGTGAAGGACCAGACGAGTATTGGAATTGCGAAGGTTGCGAGCAATATGGCGCCGGACCTTGAGGTAGCGATCGTGAAGGCTACGAGCCACGATGATGATCCAGCCAGCGAGAAGTACATCAGGGAGATTTTGAATCTGACATCGTATTCGCGTGGTTATGTGAGTGCGTGTGTGTCGGCGATTTCGAAGCGTCTGGCCAAGACGAGGGACTGGATAGTGGCGCTCAAGGCACTCATACTTGTGCACAGGTTGTTGAATGAAGGAGACCCGGTGTTTCAGGAGGAAATCTTGTATGCCACTAGAAGGGGCACGAGGCTATTGAATATGTCCGACTTTAAGGATGAAGCTCATTCGAGCTCGTGGGATCACTCGGCTTTCGTTCGAACTTATGCATTCTACTTGGACCAACGGCTGGAATTGATGTTGTTTGAGAAAAAGGGTGGTAGTACAAGGGGAAATTCCCGCGGAGATGATAGATTTGAAGGAAGAGATGACTTCAGATCTCCTCCGCCGAGGCCATATGATAATGGTTATGGTGAGTACAGGGGCGAGAGAGAGTATGGAAATTATGGTGAGATGAGGAGGTCGAGATCTTACGGGGATGTGGGGGAATCTGTGGGGAGGGAGGGGCAGGGGCAGGGGCGAAATAACAGAGGACCTGTGACCCCGTTGAGAGAAATGGTGACCGAGAGAATTTTCGGGAAGATGGGACATTTGCAGAGACTGTTGGACAGATTTTTGTCGTGCCGACCGACGGGTTTGGCGAAGAATAGTAGGATGATTTTGTATGCTTTGTACCCTCTAGTGAGGGAGAGTTTTCAGTTGTATGCAGATATTTGCGAGGTTTTGGCTGTTTTGCTTGACAAATTCTTTGATATGGAGTATTCTGACTGTATGAAGGCATTTGATGCGTATGGTAGTGCGGCCAAGCAGATTGACGAGCTGATTTCATTGTATAATTGGTGTAAAGATACAGGCGTTGCTAGGTCCTCTGAGTATCCAGAGGTGCAGAGAATTACCAGCAAGCTATTGGAGACATTGGAGGAGTTCCTTAGGGAAAGAGGGAAGAAGCCAAAGAGTCCGGAGAGGGAGCCTCCTCCGCCCGTGGCTCAAGAGGAAGAGCCAGTGCCTGATATGAATGAAATAAAAGCTCTGCCTCCACCTGAAAATTATACTCCTCCACCTCCGCCCGAGCCCGAGCCTGCACCCAAACCTCAACAACAAGTCTCAGAAGATTTGGTGAATCTGAGAGATGATGCAGTCAGTGCAGATGACCAAGGTAACAAACTAGCCCTGGCTTTGTTTGCTGGCCCAGCAGCTAATGGCGCGAACGGATCCTGGGAAGCTTTCCCTTCAGATGGACAGCCCGAAGTAACCTCTGCCTGGCAGACCCCGGCTGCTGAACCTGGAAAAGCCGATTGGGAGTTGGCTTTGGTGGAGACAGCGAGCAATTTATCAAGGCAGAAAGCAGCACTTGGTGGCGGTCTCGACCCGTTATTGCTAAATGGCATGTATGATCAAGGAATGGTCAGGCAGCACACGAGCACTGCCCAACTGAGTGGTGGAAGCGCTAGCAGTGTAGCATTGCCCGGCCCTGGAAACAGCAAAACTCCTGTACTGGCTCTTCCAGCTCCAGATGGAACTGTTCAGGCAGTGAATCAGGATCCTTTCGCCGCATCGTTAACCATTCCACCGCCTTCATACGTGCAAATGGTGGAGATGGAGAAGAAACAGCAACTGCTGGTGCAGGAGCAGCAGGTATGGCAGCAGTACTCAAGAGATGGGATGCAGGGGCAGAGCAGTTTGAACAAAATCAGTAACCCCCCAGGTTACTACACAGCGGGAATGATGGCTCCAATGCCGTACGGGATGCCTCCAGCAAACGGAATGGGCGGGTATTACTACATTCCTCAATGA

Protein sequence

MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTSYSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLNMSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSTRGNSRGDDRFEGRDDFRSPPPRPYDNGYGEYRGEREYGNYGEMRRSRSYGDVGESVGREGQGQGRNNRGPVTPLREMVTERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLDKFFDMEYSDCMKAFDAYGSAAKQIDELISLYNWCKDTGVARSSEYPEVQRITSKLLETLEEFLRERGKKPKSPEREPPPPVAQEEEPVPDMNEIKALPPPENYTPPPPPEPEPAPKPQQQVSEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAAEPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSVALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLTIPPPSYVQMVEMEKKQQLLVQEQQVWQQYSRDGMQGQSSLNKISNPPGYYTAGMMAPMPYGMPPANGMGGYYYIPQ
Homology
BLAST of MC11g0454 vs. ExPASy Swiss-Prot
Match: Q8LF20 (Putative clathrin assembly protein At2g25430 OS=Arabidopsis thaliana OX=3702 GN=At2g25430 PE=1 SV=2)

HSP 1 Score: 930.2 bits (2403), Expect = 1.2e-269
Identity = 511/668 (76.50%), Postives = 562/668 (84.13%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTS 60
           MAPS IRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTS
Sbjct: 1   MAPS-IRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTS 60

Query: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120
            SRGY+ ACV+++S+RL+KTRDW+VALKAL+LVHRLLNEGDP+FQEEILY+TRRGTR+LN
Sbjct: 61  LSRGYILACVTSVSRRLSKTRDWVVALKALMLVHRLLNEGDPIFQEEILYSTRRGTRMLN 120

Query: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKK-------GGSTRGNSRGDDRF- 180
           MSDF+DEAHSSSWDHSAFVRTYA YLDQRLEL LFE+K       GG++  +S  DDR+ 
Sbjct: 121 MSDFRDEAHSSSWDHSAFVRTYAGYLDQRLELALFERKSGVSVNSGGNSSHHSNNDDRYG 180

Query: 181 EGRDDFRSPPPRPYD--NGYG---EYRGEREYGNYGEMRRSRSYGDVGESVGREGQGQGR 240
            GRDDFRSPPPR YD  NG G   ++RG+   G  G  +RSRSYGD+ E +G  G G GR
Sbjct: 181 RGRDDFRSPPPRSYDYENGGGGGSDFRGDNN-GYGGVPKRSRSYGDMTE-MGGGGGGGGR 240

Query: 241 NNRGPVTPLREMVTERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQL 300
           + +  VTPLREM  ERIFGKMGHLQRLLDRFLS RPTGLAKNSRMIL ALYP+VRESF+L
Sbjct: 241 DEKKVVTPLREMTPERIFGKMGHLQRLLDRFLSLRPTGLAKNSRMILIALYPVVRESFKL 300

Query: 301 YADICEVLAVLLDKFFDMEYSDCMKAFDAYGSAAKQIDELISLYNWCKDTGVARSSEYPE 360
           YADICEVLAVLLDKFFDMEYSDC+KAFDAY SAAKQIDELI+ YNWCK+TGVARSSEYPE
Sbjct: 301 YADICEVLAVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGVARSSEYPE 360

Query: 361 VQRITSKLLETLEEFLRERGKKPKSPER---EPPPPVAQEEEPVPDMNEIKALPPPENYT 420
           VQRITSKLLETLEEF+R+R K+ KSPER   E PPPV +EEEP PDMNEIKALPPPENYT
Sbjct: 361 VQRITSKLLETLEEFVRDRAKRGKSPERKEIEAPPPVVEEEEPEPDMNEIKALPPPENYT 420

Query: 421 PPPPPEPEPAPKPQQQVSEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPS 480
           PPPPPEPEP P+ + Q +EDLVNLR+D V+ADDQGNK ALALFAGP  N  NG WEAF S
Sbjct: 421 PPPPPEPEPQPE-KPQFTEDLVNLREDEVTADDQGNKFALALFAGPPGN--NGKWEAFSS 480

Query: 481 DGQPEVTSAWQTPAAEPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQ 540
           +G   VTSAWQ PAAEPGKADWELALVET SNL +Q AALGGG D LLLNGMYDQGMVRQ
Sbjct: 481 NG---VTSAWQNPAAEPGKADWELALVETTSNLEKQTAALGGGFDNLLLNGMYDQGMVRQ 540

Query: 541 HTSTAQLSGGSASSVALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLTIPPPSYVQMV 600
           H ST+QL+GGSASSVALP PG +   VLALPAPDGTV+ VNQDPFAASLTIPPPSYVQM 
Sbjct: 541 HVSTSQLTGGSASSVALPLPGKTNNQVLALPAPDGTVEKVNQDPFAASLTIPPPSYVQMA 600

Query: 601 EMEKKQQLLVQEQQVWQQYSRDGMQGQSSLNKISNPPGYYTAGMMAPMP-YGMPPANGMG 648
           EMEKKQ LL QEQQ+WQQY RDGM+GQ+SL K++            P+P YGMPP NGMG
Sbjct: 601 EMEKKQYLLSQEQQLWQQYQRDGMRGQASLAKMNT----------GPVPAYGMPPVNGMG 649

BLAST of MC11g0454 vs. ExPASy Swiss-Prot
Match: Q8S9J8 (Probable clathrin assembly protein At4g32285 OS=Arabidopsis thaliana OX=3702 GN=At4g32285 PE=1 SV=2)

HSP 1 Score: 891.0 bits (2301), Expect = 8.3e-258
Identity = 485/657 (73.82%), Postives = 538/657 (81.89%), Query Frame = 0

Query: 5   TIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTSYSRG 64
           ++RKAIG VKDQTSIGIAKVASNMAPDLEVAIVKATSHDDD +S+KYIREIL+LTS SRG
Sbjct: 4   SMRKAIGVVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDQSSDKYIREILSLTSLSRG 63

Query: 65  YVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLNMSDF 124
           YV ACV+++S+RL KTRDWIVALKAL+LVHRLLNEGDP+FQEEILYATRRGTR+LNMSDF
Sbjct: 64  YVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRILNMSDF 123

Query: 125 KDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKK-----GGSTRGNSRGDDRF-EGRDDF 184
           +DEAHSSSWDHSAFVRTYA YLDQRLEL LFE++     G S+   S GDD +   RDDF
Sbjct: 124 RDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDGYNRSRDDF 183

Query: 185 RSPPPRPYDNGYGEYRGEREYGNYGEMRRSRSYGDVGESVGREGQGQGRNNRGPVTPLRE 244
           RSPPPR YD   G          +G  +RSRS+GDV E   RE        +  VTPLRE
Sbjct: 184 RSPPPRTYDYETG--------NGFGMPKRSRSFGDVNEIGARE-------EKKSVTPLRE 243

Query: 245 MVTERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVL 304
           M  ERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMIL A+YP+V+ESF+LYADICEVLAVL
Sbjct: 244 MTPERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVL 303

Query: 305 LDKFFDMEYSDCMKAFDAYGSAAKQIDELISLYNWCKDTGVARSSEYPEVQRITSKLLET 364
           LDKFFDMEY+DC+KAFDAY SAAKQIDELI+ Y+WCKDTGVARSSEYPEVQRITSKLLET
Sbjct: 304 LDKFFDMEYTDCVKAFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLET 363

Query: 365 LEEFLRERGKKPKSPERE----PPPPVAQEEEPVPDMNEIKALPPPENYTPPPPPEPEPA 424
           LEEF+R+R K+ KSPER+    PP P    EEPV DMNEIKALPPPEN+TPPPPP PEP 
Sbjct: 364 LEEFVRDRAKRAKSPERKEIEAPPAPAPPVEEPV-DMNEIKALPPPENHTPPPPPAPEPK 423

Query: 425 PKPQQQVSEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAW 484
           P+ Q QV++DLVNLR+D VS DDQGNK ALALFAGP AN  NG WEAF SD    VTSAW
Sbjct: 424 PQ-QPQVTDDLVNLREDDVSGDDQGNKFALALFAGPPAN--NGKWEAFSSDN--NVTSAW 483

Query: 485 QTPAAEPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGG 544
           Q PAAE GKADWELALVETASNL  QKAA+GGGLDPLLLNGMYDQG VRQH ST++L+GG
Sbjct: 484 QNPAAELGKADWELALVETASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQHVSTSELTGG 543

Query: 545 SASSVALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLTIPPPSYVQMVEMEKKQQLLV 604
           S+SSVALP PG   + +LALPAPDGTVQ VNQDPFAASLTIPPPSYVQM EM+KKQ LL 
Sbjct: 544 SSSSVALPLPGKVNSHILALPAPDGTVQKVNQDPFAASLTIPPPSYVQMAEMDKKQYLLT 603

Query: 605 QEQQVWQQYSRDGMQGQSSLNKISNPPGYYTAGMMAPMPYGMPPANGMG----GYYY 648
           QEQQ+WQQY ++GM+GQ+SL K++             MPYGMPP NGMG    GYYY
Sbjct: 604 QEQQLWQQYQQEGMRGQASLAKMNT--------AQTAMPYGMPPVNGMGPSPMGYYY 631

BLAST of MC11g0454 vs. ExPASy Swiss-Prot
Match: Q9SA65 (Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=At1g03050 PE=2 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 2.2e-133
Identity = 297/663 (44.80%), Postives = 398/663 (60.03%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMA--PDLEVAIVKATSHDDDPASEKYIREILNL 60
           M  S  ++AIGAVKDQTS+G+AKV    A   +L+VAIVKAT H++ PA EKYIREIL+L
Sbjct: 1   MGSSKFKRAIGAVKDQTSVGLAKVNGRSASLSELDVAIVKATRHEEFPAEEKYIREILSL 60

Query: 61  TSYSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRL 120
           TSYSR Y++ACVS +S+RL KT+ W VALK LIL+ RLL EGD  +++EI +ATRRGTRL
Sbjct: 61  TSYSRSYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGDQAYEQEIFFATRRGTRL 120

Query: 121 LNMSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSTRGNSRGDDRFEGRDDF 180
           LNMSDF+D + S+SWD+SAFVRTYA YLD+RL+  +        R   RG     G  D 
Sbjct: 121 LNMSDFRDVSRSNSWDYSAFVRTYALYLDERLDFRM------QARHGKRGVYCVGGEAD- 180

Query: 181 RSPPPRPYDNGYGEYRGEREYGNYGEMRRSRSYGDVGESVGREGQGQGRNNRGPVTPLRE 240
                                    E  + ++  D+  ++    Q           P+ E
Sbjct: 181 -------------------------EEEQDQAAADLSTAIVVRSQ-----------PIAE 240

Query: 241 MVTERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVL 300
           M TE+IF ++ HLQ+LLDRFL+CRPTG A+N+R+++ ALYP+V+ESFQ+Y D+ E++ +L
Sbjct: 241 MKTEQIFIRIQHLQQLLDRFLACRPTGNARNNRVVIVALYPIVKESFQIYYDVTEIMGIL 300

Query: 301 LDKFFDMEYSDCMKAFDAYGSAAKQIDELISLYNWCKDTGVARSSEYPEVQRITSKLLET 360
           +++F +++  D +K +D +   +KQ +EL   Y+WCK+ G+ARSSEYPE+++IT K L+ 
Sbjct: 301 IERFMELDIPDSIKVYDIFCRVSKQFEELDQFYSWCKNMGIARSSEYPEIEKITQKKLDL 360

Query: 361 LEEFLRER--------GKKPKSPEREPPPPVAQEE--EPVPDMNEIKALPPPENYTPPPP 420
           ++EF+R++         K  KS   E       EE  E   DMN IKAL       P PP
Sbjct: 361 MDEFIRDKSALEHTKQSKSVKSEADEDDDEARTEEVNEEQEDMNAIKAL-------PEPP 420

Query: 421 PEPEPAPKPQQQVSE------------DLVNL-RDDAVSADDQGNKLALALFAGPAANGA 480
           P+ E   KP+++  E            DL++L   +   A   G+ LALALF GP A+G+
Sbjct: 421 PKEEDDVKPEEEAKEEVIIEKKQEEMGDLLDLGNTNGGEAGQAGDSLALALFDGPYASGS 480

Query: 481 NG----SWEAFPSDGQPEVTSAWQTPAAEPGKADWELALVETASNLSRQKAALGGGLDPL 540
                  WEAF  D                  ADWE ALV+TA+NLS QK+ LGGG D L
Sbjct: 481 GSESGPGWEAFKDD-----------------SADWETALVQTATNLSGQKSELGGGFDML 540

Query: 541 LLNGMYDQGMVRQ--HTSTAQLSGGSASSVALPGPGNSKTPVLALPAPD------GTVQA 600
           LLNGMY  G V     TSTA  + GSASS+A    G     +LALPAP       G + +
Sbjct: 541 LLNGMYQHGAVNAAVKTSTAYGASGSASSMAFGSAGRPAATMLALPAPSTANGNAGNINS 596

Query: 601 -VNQDPFAASLTIPPPSYVQMVEMEKKQQLLVQEQQVWQQYSRDGMQGQSSLNKISNPPG 626
            V  DPFAASL + PP+YVQM +MEKKQ++L++EQ +W QYSRDG QG  +L +  N P 
Sbjct: 601 PVPMDPFAASLEVAPPAYVQMNDMEKKQRMLMEEQMMWDQYSRDGRQGHMNLRQNQNQPY 596

BLAST of MC11g0454 vs. ExPASy Swiss-Prot
Match: Q8GX47 (Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=At4g02650 PE=2 SV=2)

HSP 1 Score: 463.4 bits (1191), Expect = 4.3e-129
Identity = 293/664 (44.13%), Postives = 390/664 (58.73%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVA--SNMAPDLEVAIVKATSHDDDPASEKYIREILNL 60
           M  S +++AIGAVKDQTS+G+AKV   S+   +LE+A+VKAT HDD PA +KYIREIL L
Sbjct: 1   MGSSKLKRAIGAVKDQTSVGLAKVGGRSSSLTELEIAVVKATRHDDYPAEDKYIREILCL 60

Query: 61  TSYSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRL 120
           TSYSR YVSACV+ +S+RL KT++W VALK LIL+ RLL +GD  +++EI +ATRRGTRL
Sbjct: 61  TSYSRNYVSACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEIFFATRRGTRL 120

Query: 121 LNMSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSTRGNSRGDDRFEGRDDF 180
           LNMSDF+D + S SWD+SAFVRTYA YLD+RL+  +  ++G    G   G D   G +D 
Sbjct: 121 LNMSDFRDASQSDSWDYSAFVRTYALYLDERLDYRMQGRRGKKKSGGGGGGDGDSGEED- 180

Query: 181 RSPPPRPYDNGYGEYRGEREYGNYGEMRRSRSYGDVGESVGREGQGQGRNNRGPVTPLRE 240
                                 + G     RS   V +S                 P+ E
Sbjct: 181 ---------------------DHRGTSNDIRSKAIVVKS----------------KPVAE 240

Query: 241 MVTERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVL 300
           M TE+IF ++ HLQ+LLDRFL+CRPTG AKN+R+++ A+YP+V+ESFQLY +I E++ VL
Sbjct: 241 MKTEKIFNRVQHLQQLLDRFLACRPTGNAKNNRVVIVAMYPIVKESFQLYYNITEIMGVL 300

Query: 301 LDKFFDMEYSDCMKAFDAYGSAAKQIDELISLYNWCKDTGVARSSEYPEVQRITSKLLET 360
           +++F +++  D +K ++ +   +KQ DEL   Y WCK+  VARSSEYPE+++IT K L+ 
Sbjct: 301 IERFMELDIHDSIKVYEIFCRVSKQFDELDPFYGWCKNMAVARSSEYPELEKITQKKLDL 360

Query: 361 LEEFLRERG------------KKPKSPEREPPPPVAQEEEPVPDMNEIKALPPPENYTPP 420
           ++EF+R++             +  KS E E      QE +   D+N IKALP PE     
Sbjct: 361 MDEFIRDKSALAAQTTKSSSKRSNKSEEEESKTEYIQENQ--EDLNSIKALPAPEQKEEE 420

Query: 421 PPPEPEPAPKPQQQV------SEDLVNLRDDA-VSADDQGNKLALALFAGPAA--NGANG 480
              E     K  ++V        DL++L D+A V+A   G+ LALALF G     + +  
Sbjct: 421 EEEEKMETKKDVEEVVSRQDQEGDLLDLTDEAGVTAGTVGDSLALALFDGVVGTESASGP 480

Query: 481 SWEAFPSDGQPEVTSAWQTPAAEPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMY 540
            WEAF                     ADWE  LV +A+ LS QK+ LGGG D LLL+GMY
Sbjct: 481 GWEAF-----------------NDNSADWETDLVRSATRLSGQKSELGGGFDTLLLDGMY 540

Query: 541 DQGMVRQ--HTSTAQLSGGSASSVALPGPGNSKTPVLALPAPDGTVQA-----VNQDPFA 600
             G V     TSTA  S GSASSVA    G+    +LALPAP  T        V  DPFA
Sbjct: 541 QYGAVNAAVKTSTAYGSSGSASSVAFGSAGSPAASMLALPAPPPTANGNRNSPVMVDPFA 600

Query: 601 ASLTIPPPSYVQMVEMEKKQQLLVQEQQVWQQYSRDGMQGQSSLNKISNPPGYYTAGMMA 635
           ASL + PP+YVQM +MEKKQ+LL++EQ +W QY+R G QG  +  +      Y     M 
Sbjct: 601 ASLEVAPPAYVQMNDMEKKQRLLMEEQIMWDQYNRSGRQGHMNFGQNQQQQYYQLPYSMG 607

BLAST of MC11g0454 vs. ExPASy Swiss-Prot
Match: Q9ZVN6 (Clathrin coat assembly protein AP180 OS=Arabidopsis thaliana OX=3702 GN=AP180 PE=1 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 8.8e-66
Identity = 155/418 (37.08%), Postives = 233/418 (55.74%), Query Frame = 0

Query: 3   PSTIRKAIGAVKDQTSIGIAKVASNMA-----PDLEVAIVKATSHDDD-PASEKYIREIL 62
           PS ++KAIGAVKDQTSI +AKVA+          LEVAI+KATSHD++ P  ++ + EIL
Sbjct: 2   PSKLKKAIGAVKDQTSISLAKVANGATGGGDLTTLEVAILKATSHDEEVPIDDRLVTEIL 61

Query: 63  NLTSYSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGT 122
            + S  + + ++C +AI +R+ +TR+WIVALK+L+LV R+  +GDP F  E+L+A +RG 
Sbjct: 62  GIISSKKSHAASCAAAIGRRIGRTRNWIVALKSLVLVLRIFQDGDPYFPREVLHAMKRGA 121

Query: 123 RLLNMSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSTRGNSRGDDRFEGRD 182
           ++LN+S F+D+++S  WD +AFVRT+A YLD+RL+  L  K                   
Sbjct: 122 KILNLSSFRDDSNSCPWDFTAFVRTFALYLDERLDCFLTGKL------------------ 181

Query: 183 DFRSPPPRPYDNGYGEYRGEREYGNYGEMRRSRSYGDVGESVGREGQGQGRNNRGPVTPL 242
                           Y    + G       +RS         R     G  +  P   +
Sbjct: 182 -------------QRRYTNREQTGRISTNSTTRS---------RFNPKAGIKSHEPA--V 241

Query: 243 REMVTERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLA 302
           R+M    +  K+ + Q+LLDR ++ RPTG AK +R++  +LY +++ESF LY DI + LA
Sbjct: 242 RDMKPVMLLDKITYWQKLLDRAIATRPTGDAKANRLVKMSLYAVMQESFDLYRDISDGLA 301

Query: 303 VLLDKFFDMEYSDCMKAFDAYGSAAKQIDELISLYNWCKDTGVARSSEYPEVQRITSKLL 362
           +LLD FF ++Y  C+ AF A   A+KQ +EL + Y+  K  G+ R+SEYP +Q+I+ +LL
Sbjct: 302 LLLDSFFHLQYQSCINAFQACVRASKQFEELNAFYDLSKSIGIGRTSEYPSIQKISLELL 356

Query: 363 ETLEEFLRERGKKPKSPEREPPPPVAQEEEPVPDMNEIKALPPPENYTPPPPPEPEPA 415
           ETL+EFL+++   P S                         P P ++ PPPP   + A
Sbjct: 362 ETLQEFLKDQSSFPASS---------------------GLYPSPNSFLPPPPSSKDSA 356

BLAST of MC11g0454 vs. NCBI nr
Match: XP_008466416.1 (PREDICTED: putative clathrin assembly protein At2g25430 [Cucumis melo])

HSP 1 Score: 1211 bits (3132), Expect = 0.0
Identity = 616/653 (94.33%), Postives = 636/653 (97.40%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTS 60
           MAPSTIRKA+GA+KDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREIL+LTS
Sbjct: 1   MAPSTIRKAVGALKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60

Query: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120
           YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN
Sbjct: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120

Query: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSTRGNSRGDDRFEGRDDFRS 180
           MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGS RGNSRGDDRF+GRDDFRS
Sbjct: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRDDFRS 180

Query: 181 PPPRPYDNGYGEYRGEREYGNYGEMRRSRSYGDVGESVGREGQGQGRNNRGPVTPLREMV 240
           PPPRPYDNGYGEYRGEREYGNYG MRRSRSYGDVGES GR+GQGQGRNN+GPVTPLREM 
Sbjct: 181 PPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGESTGRDGQGQGRNNKGPVTPLREMT 240

Query: 241 TERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300
            ER+FGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD
Sbjct: 241 IERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300

Query: 301 KFFDMEYSDCMKAFDAYGSAAKQIDELISLYNWCKDTGVARSSEYPEVQRITSKLLETLE 360
           KFFDMEYSDCMKAFDAYGSAAKQIDELI+ YNWCKDTGVARSSEYPEVQRITSKLLETLE
Sbjct: 301 KFFDMEYSDCMKAFDAYGSAAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE 360

Query: 361 EFLRERGKKPKSPEREPPPPVAQEEEPVPDMNEIKALPPPENYTPPPP-PEPEPAPKPQQ 420
           EFLRERGK+PKSPEREPPPP  +EEEP PDMNEIKALPPPENYTPPPP PEP+PAPKPQ 
Sbjct: 361 EFLRERGKRPKSPEREPPPPAPEEEEPAPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP 420

Query: 421 QVSEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480
           QV++DLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA
Sbjct: 421 QVTDDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480

Query: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV 540
           EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV
Sbjct: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV 540

Query: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLTIPPPSYVQMVEMEKKQQLLVQEQQV 600
           ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASL++PPPSYVQMVEMEKKQ LL+QEQQ+
Sbjct: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHLLMQEQQL 600

Query: 601 WQQYSRDGMQGQSSLNKISNPPGYYTAGM--MAPMPYGMPPANGMGGYYYIPQ 650
           WQQY+RDGMQGQSSLNKISNPPGYY  G+  MAPMPYGMPP NGMGGYYY+PQ
Sbjct: 601 WQQYARDGMQGQSSLNKISNPPGYYNMGVAPMAPMPYGMPPMNGMGGYYYVPQ 653

BLAST of MC11g0454 vs. NCBI nr
Match: XP_038898099.1 (putative clathrin assembly protein At2g25430 [Benincasa hispida])

HSP 1 Score: 1207 bits (3122), Expect = 0.0
Identity = 616/653 (94.33%), Postives = 632/653 (96.78%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTS 60
           MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREIL+LTS
Sbjct: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60

Query: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120
           YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN
Sbjct: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120

Query: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSTRGNSRGDDRFEGRDDFRS 180
           MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSTRGNSRGDDRF+GRDDFRS
Sbjct: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSTRGNSRGDDRFDGRDDFRS 180

Query: 181 PPPRPYDNGYGEYRGEREYGNYGEMRRSRSYGDVGESVGREGQGQGRNNRGPVTPLREMV 240
           PPPRPYDN YGEYRGEREYGNYG MRRSRSYGDVGESVGR+GQGQG NN+GPVTPLREM 
Sbjct: 181 PPPRPYDNSYGEYRGEREYGNYGGMRRSRSYGDVGESVGRDGQGQGHNNKGPVTPLREMA 240

Query: 241 TERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300
            ERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD
Sbjct: 241 IERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300

Query: 301 KFFDMEYSDCMKAFDAYGSAAKQIDELISLYNWCKDTGVARSSEYPEVQRITSKLLETLE 360
           KFFDMEYSDCMKAFDAYGSAAKQIDELI+ YNWCKDTGVARSSEYPEVQRITSKLLETLE
Sbjct: 301 KFFDMEYSDCMKAFDAYGSAAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE 360

Query: 361 EFLRERGKKPKSPEREPPPPVAQEEEPVPDMNEIKALPPPENYTPPPP-PEPEPAPKPQQ 420
           EFLRERGK+PKSPEREPPPP  +EEEPVPDMNEIKALPPPENYTPPPP PEP+PAPKPQ 
Sbjct: 361 EFLRERGKRPKSPEREPPPPAPEEEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP 420

Query: 421 QVSEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480
           QV+EDLVNLRDD VSADDQGNKLALALFAGP ANGANGSWEAFPSDGQPEVTSAWQTPAA
Sbjct: 421 QVTEDLVNLRDDGVSADDQGNKLALALFAGPVANGANGSWEAFPSDGQPEVTSAWQTPAA 480

Query: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV 540
           EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV
Sbjct: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV 540

Query: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLTIPPPSYVQMVEMEKKQQLLVQEQQV 600
           ALPGPGNSKTP+LALPAPDGTVQ VNQDPFA SL++P PSYVQMVEMEKKQ LL+QEQQ+
Sbjct: 541 ALPGPGNSKTPILALPAPDGTVQTVNQDPFATSLSVPAPSYVQMVEMEKKQHLLMQEQQL 600

Query: 601 WQQYSRDGMQGQSSLNKISNPPGYYTAGM--MAPMPYGMPPANGMGGYYYIPQ 650
           WQQY+RDGMQGQSSLNKISNPPGYY  GM  MAPMPYGMPP NGMGGYYY+PQ
Sbjct: 601 WQQYARDGMQGQSSLNKISNPPGYYNTGMAPMAPMPYGMPPMNGMGGYYYVPQ 653

BLAST of MC11g0454 vs. NCBI nr
Match: XP_004136352.1 (putative clathrin assembly protein At2g25430 [Cucumis sativus] >KGN60096.1 hypothetical protein Csa_001389 [Cucumis sativus])

HSP 1 Score: 1201 bits (3107), Expect = 0.0
Identity = 612/653 (93.72%), Postives = 633/653 (96.94%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTS 60
           MAPSTIRKA+GA+KDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREIL+LTS
Sbjct: 1   MAPSTIRKAVGALKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60

Query: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120
           YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN
Sbjct: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120

Query: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSTRGNSRGDDRFEGRDDFRS 180
           MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGS RGNSRGDDRF+GRD+FRS
Sbjct: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRDEFRS 180

Query: 181 PPPRPYDNGYGEYRGEREYGNYGEMRRSRSYGDVGESVGREGQGQGRNNRGPVTPLREMV 240
           PPPRPYDNGYGEYRGEREYGNYG MRRSRSYGDVGES GR+GQGQGR N+GPVTPLREM 
Sbjct: 181 PPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGESTGRDGQGQGRINKGPVTPLREMT 240

Query: 241 TERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300
            ER+FGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD
Sbjct: 241 IERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300

Query: 301 KFFDMEYSDCMKAFDAYGSAAKQIDELISLYNWCKDTGVARSSEYPEVQRITSKLLETLE 360
           KFFDMEYSDCMKAFDAYGSAAKQIDELI+ YNWCK+TGVARSSEYPEVQRITSKLLETLE
Sbjct: 301 KFFDMEYSDCMKAFDAYGSAAKQIDELIAFYNWCKETGVARSSEYPEVQRITSKLLETLE 360

Query: 361 EFLRERGKKPKSPEREPPPPVAQEEEPVPDMNEIKALPPPENYTPPPP-PEPEPAPKPQQ 420
           EFLRERGK+PKSPEREPPPP  +EEEP PDMNEIKALPPPENYTPPPP PEP+PAPKPQ 
Sbjct: 361 EFLRERGKRPKSPEREPPPPAPEEEEPAPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP 420

Query: 421 QVSEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480
           QV++DLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA
Sbjct: 421 QVTDDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480

Query: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV 540
           EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV
Sbjct: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV 540

Query: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLTIPPPSYVQMVEMEKKQQLLVQEQQV 600
           ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASL++PPPSYVQMVEMEKKQ LL+QEQQ+
Sbjct: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHLLMQEQQL 600

Query: 601 WQQYSRDGMQGQSSLNKISNPPGYYTAGM--MAPMPYGMPPANGMGGYYYIPQ 650
           WQQY+RDGMQGQSSL KISNPPGYY  G   MAPMPYGMPP NGMGGYYY+PQ
Sbjct: 601 WQQYARDGMQGQSSLTKISNPPGYYNMGAAPMAPMPYGMPPMNGMGGYYYVPQ 653

BLAST of MC11g0454 vs. NCBI nr
Match: KAG6591568.1 (putative clathrin assembly protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG6591605.1 putative clathrin assembly protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1175 bits (3040), Expect = 0.0
Identity = 610/653 (93.42%), Postives = 626/653 (95.87%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTS 60
           MAPSTIRKAIGAVKDQTSIGIAKVASN APDLEVAIVKATSHDDDPASEKYIREIL+LTS
Sbjct: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNTAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60

Query: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120
           YSRGYV+ACV AISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN
Sbjct: 61  YSRGYVNACVVAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120

Query: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSTRGNSRGDDRFEGRDDFRS 180
           MSDFKDEAHSSSWDHSAF+RTYAFYLDQRLEL+LFEKKGGS RGNSRGDDRF+GRDDFRS
Sbjct: 121 MSDFKDEAHSSSWDHSAFIRTYAFYLDQRLELVLFEKKGGSARGNSRGDDRFDGRDDFRS 180

Query: 181 PPPRPYDNGYGEYRGEREYGNYGEMRRSRSYGDVGESVGREGQGQGRNNRGPVTPLREMV 240
           PPPRPYDNGYGEYRGEREYGNYG MRRSRS+GDVGES+ REGQG+   N+GPVTPLREM 
Sbjct: 181 PPPRPYDNGYGEYRGEREYGNYGGMRRSRSFGDVGESMEREGQGR---NKGPVTPLREMP 240

Query: 241 TERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300
            ERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD
Sbjct: 241 IERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300

Query: 301 KFFDMEYSDCMKAFDAYGSAAKQIDELISLYNWCKDTGVARSSEYPEVQRITSKLLETLE 360
           KFFDMEYSD MKAFDAYGSAAKQIDELI+ YNWCKDTGVARSSEYPEVQRITSKLLETLE
Sbjct: 301 KFFDMEYSDSMKAFDAYGSAAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE 360

Query: 361 EFLRERGKKPKSPEREPPPPVAQEEEPVPDMNEIKALPPPENYTPPPP-PEPEPAPKPQQ 420
           EFLRERGK PKSPEREPPPP  QEEEPVPDMNEIKALPPPENYTPPPP PEP+PAPKPQ 
Sbjct: 361 EFLRERGKAPKSPEREPPPPAPQEEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP 420

Query: 421 QVSEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480
           QV+EDLVNLRDDAVSAD QGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA
Sbjct: 421 QVTEDLVNLRDDAVSADAQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480

Query: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV 540
           EPGKADWEL LVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV
Sbjct: 481 EPGKADWELTLVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV 540

Query: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLTIPPPSYVQMVEMEKKQQLLVQEQQV 600
           ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASL+IPPPSYVQMVEMEKKQ LLVQEQQ+
Sbjct: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSIPPPSYVQMVEMEKKQHLLVQEQQL 600

Query: 601 WQQYSRDGMQGQSSLNKISNPPGYYT--AGMMAPMPYGMPPANGMGGYYYIPQ 650
           WQQY+RDGMQGQSSLNK+SNPPGYY   AG MAPMPYGMPP NG G YYYIPQ
Sbjct: 601 WQQYARDGMQGQSSLNKMSNPPGYYNTAAGPMAPMPYGMPPMNGSG-YYYIPQ 649

BLAST of MC11g0454 vs. NCBI nr
Match: XP_022976123.1 (putative clathrin assembly protein At2g25430 [Cucurbita maxima])

HSP 1 Score: 1172 bits (3032), Expect = 0.0
Identity = 609/653 (93.26%), Postives = 627/653 (96.02%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTS 60
           MAPSTIRKAIGAVKDQTSIGIAKVASN APDLEVAIVKATSHDDDPASEKYIREIL+LTS
Sbjct: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNTAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60

Query: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120
           YSRGYV+ACV AISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN
Sbjct: 61  YSRGYVNACVVAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120

Query: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSTRGNSRGDDRFEGRDDFRS 180
           MSDFKDEAHSSSWDHSAF+RTYAFYLDQRLEL+LFEKKGGS RGNSRGDDRF+GRDDFRS
Sbjct: 121 MSDFKDEAHSSSWDHSAFIRTYAFYLDQRLELVLFEKKGGSARGNSRGDDRFDGRDDFRS 180

Query: 181 PPPRPYDNGYGEYRGEREYGNYGEMRRSRSYGDVGESVGREGQGQGRNNRGPVTPLREMV 240
           PPPRPY+NGYGEYRGERE GNYG MRRSRS+GDVGES+ REGQGQGRN +GPVTPLREM 
Sbjct: 181 PPPRPYENGYGEYRGERENGNYGGMRRSRSFGDVGESMEREGQGQGRN-KGPVTPLREMP 240

Query: 241 TERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300
            ERIFGKMGHLQRLLDRFLSCRPTGLAK+SRMILYALYPLVRESFQLYADICEVLAVLLD
Sbjct: 241 IERIFGKMGHLQRLLDRFLSCRPTGLAKSSRMILYALYPLVRESFQLYADICEVLAVLLD 300

Query: 301 KFFDMEYSDCMKAFDAYGSAAKQIDELISLYNWCKDTGVARSSEYPEVQRITSKLLETLE 360
           KFFDMEYSD MKAFDAYGSAAKQIDELI+ YNWCKDTGVARSSEYPEVQRITSKLLETLE
Sbjct: 301 KFFDMEYSDSMKAFDAYGSAAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE 360

Query: 361 EFLRERGKKPKSPEREPPPPVAQEEEPVPDMNEIKALPPPENYTPPPP-PEPEPAPKPQQ 420
           EFLRERGK PKSPEREPPPP  QEEEPVPDMNEIKALPPPENYTPPPP PEP+PAPKPQ 
Sbjct: 361 EFLRERGKAPKSPEREPPPPAPQEEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP 420

Query: 421 QVSEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480
           QV+EDLVNLRDDAVSAD QGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA
Sbjct: 421 QVTEDLVNLRDDAVSADAQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480

Query: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV 540
           EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLS GSASSV
Sbjct: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSSGSASSV 540

Query: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLTIPPPSYVQMVEMEKKQQLLVQEQQV 600
           ALPGPGNSKTPVLALPAPDGTVQA+NQDPFAASL+IPPPSYVQMVEMEKKQ LLVQEQQ+
Sbjct: 541 ALPGPGNSKTPVLALPAPDGTVQALNQDPFAASLSIPPPSYVQMVEMEKKQHLLVQEQQL 600

Query: 601 WQQYSRDGMQGQSSLNKISNPPGYYT--AGMMAPMPYGMPPANGMGGYYYIPQ 650
           WQQY+RDGMQGQSSLNK+SNPPGYY   AG MAPMPYGMPP NG G YYYIPQ
Sbjct: 601 WQQYARDGMQGQSSLNKMSNPPGYYNTAAGPMAPMPYGMPPMNGSG-YYYIPQ 651

BLAST of MC11g0454 vs. ExPASy TrEMBL
Match: A0A1S3CRD1 (putative clathrin assembly protein At2g25430 OS=Cucumis melo OX=3656 GN=LOC103503830 PE=4 SV=1)

HSP 1 Score: 1211 bits (3132), Expect = 0.0
Identity = 616/653 (94.33%), Postives = 636/653 (97.40%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTS 60
           MAPSTIRKA+GA+KDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREIL+LTS
Sbjct: 1   MAPSTIRKAVGALKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60

Query: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120
           YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN
Sbjct: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120

Query: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSTRGNSRGDDRFEGRDDFRS 180
           MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGS RGNSRGDDRF+GRDDFRS
Sbjct: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRDDFRS 180

Query: 181 PPPRPYDNGYGEYRGEREYGNYGEMRRSRSYGDVGESVGREGQGQGRNNRGPVTPLREMV 240
           PPPRPYDNGYGEYRGEREYGNYG MRRSRSYGDVGES GR+GQGQGRNN+GPVTPLREM 
Sbjct: 181 PPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGESTGRDGQGQGRNNKGPVTPLREMT 240

Query: 241 TERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300
            ER+FGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD
Sbjct: 241 IERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300

Query: 301 KFFDMEYSDCMKAFDAYGSAAKQIDELISLYNWCKDTGVARSSEYPEVQRITSKLLETLE 360
           KFFDMEYSDCMKAFDAYGSAAKQIDELI+ YNWCKDTGVARSSEYPEVQRITSKLLETLE
Sbjct: 301 KFFDMEYSDCMKAFDAYGSAAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE 360

Query: 361 EFLRERGKKPKSPEREPPPPVAQEEEPVPDMNEIKALPPPENYTPPPP-PEPEPAPKPQQ 420
           EFLRERGK+PKSPEREPPPP  +EEEP PDMNEIKALPPPENYTPPPP PEP+PAPKPQ 
Sbjct: 361 EFLRERGKRPKSPEREPPPPAPEEEEPAPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP 420

Query: 421 QVSEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480
           QV++DLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA
Sbjct: 421 QVTDDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480

Query: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV 540
           EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV
Sbjct: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV 540

Query: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLTIPPPSYVQMVEMEKKQQLLVQEQQV 600
           ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASL++PPPSYVQMVEMEKKQ LL+QEQQ+
Sbjct: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHLLMQEQQL 600

Query: 601 WQQYSRDGMQGQSSLNKISNPPGYYTAGM--MAPMPYGMPPANGMGGYYYIPQ 650
           WQQY+RDGMQGQSSLNKISNPPGYY  G+  MAPMPYGMPP NGMGGYYY+PQ
Sbjct: 601 WQQYARDGMQGQSSLNKISNPPGYYNMGVAPMAPMPYGMPPMNGMGGYYYVPQ 653

BLAST of MC11g0454 vs. ExPASy TrEMBL
Match: A0A0A0LJA4 (ENTH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G877670 PE=4 SV=1)

HSP 1 Score: 1201 bits (3107), Expect = 0.0
Identity = 612/653 (93.72%), Postives = 633/653 (96.94%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTS 60
           MAPSTIRKA+GA+KDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREIL+LTS
Sbjct: 1   MAPSTIRKAVGALKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60

Query: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120
           YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN
Sbjct: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120

Query: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSTRGNSRGDDRFEGRDDFRS 180
           MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGS RGNSRGDDRF+GRD+FRS
Sbjct: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSARGNSRGDDRFDGRDEFRS 180

Query: 181 PPPRPYDNGYGEYRGEREYGNYGEMRRSRSYGDVGESVGREGQGQGRNNRGPVTPLREMV 240
           PPPRPYDNGYGEYRGEREYGNYG MRRSRSYGDVGES GR+GQGQGR N+GPVTPLREM 
Sbjct: 181 PPPRPYDNGYGEYRGEREYGNYGGMRRSRSYGDVGESTGRDGQGQGRINKGPVTPLREMT 240

Query: 241 TERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300
            ER+FGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD
Sbjct: 241 IERVFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300

Query: 301 KFFDMEYSDCMKAFDAYGSAAKQIDELISLYNWCKDTGVARSSEYPEVQRITSKLLETLE 360
           KFFDMEYSDCMKAFDAYGSAAKQIDELI+ YNWCK+TGVARSSEYPEVQRITSKLLETLE
Sbjct: 301 KFFDMEYSDCMKAFDAYGSAAKQIDELIAFYNWCKETGVARSSEYPEVQRITSKLLETLE 360

Query: 361 EFLRERGKKPKSPEREPPPPVAQEEEPVPDMNEIKALPPPENYTPPPP-PEPEPAPKPQQ 420
           EFLRERGK+PKSPEREPPPP  +EEEP PDMNEIKALPPPENYTPPPP PEP+PAPKPQ 
Sbjct: 361 EFLRERGKRPKSPEREPPPPAPEEEEPAPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP 420

Query: 421 QVSEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480
           QV++DLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA
Sbjct: 421 QVTDDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480

Query: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV 540
           EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV
Sbjct: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV 540

Query: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLTIPPPSYVQMVEMEKKQQLLVQEQQV 600
           ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASL++PPPSYVQMVEMEKKQ LL+QEQQ+
Sbjct: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHLLMQEQQL 600

Query: 601 WQQYSRDGMQGQSSLNKISNPPGYYTAGM--MAPMPYGMPPANGMGGYYYIPQ 650
           WQQY+RDGMQGQSSL KISNPPGYY  G   MAPMPYGMPP NGMGGYYY+PQ
Sbjct: 601 WQQYARDGMQGQSSLTKISNPPGYYNMGAAPMAPMPYGMPPMNGMGGYYYVPQ 653

BLAST of MC11g0454 vs. ExPASy TrEMBL
Match: A0A6J1IL73 (putative clathrin assembly protein At2g25430 OS=Cucurbita maxima OX=3661 GN=LOC111476611 PE=4 SV=1)

HSP 1 Score: 1172 bits (3032), Expect = 0.0
Identity = 609/653 (93.26%), Postives = 627/653 (96.02%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTS 60
           MAPSTIRKAIGAVKDQTSIGIAKVASN APDLEVAIVKATSHDDDPASEKYIREIL+LTS
Sbjct: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNTAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60

Query: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120
           YSRGYV+ACV AISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN
Sbjct: 61  YSRGYVNACVVAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120

Query: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSTRGNSRGDDRFEGRDDFRS 180
           MSDFKDEAHSSSWDHSAF+RTYAFYLDQRLEL+LFEKKGGS RGNSRGDDRF+GRDDFRS
Sbjct: 121 MSDFKDEAHSSSWDHSAFIRTYAFYLDQRLELVLFEKKGGSARGNSRGDDRFDGRDDFRS 180

Query: 181 PPPRPYDNGYGEYRGEREYGNYGEMRRSRSYGDVGESVGREGQGQGRNNRGPVTPLREMV 240
           PPPRPY+NGYGEYRGERE GNYG MRRSRS+GDVGES+ REGQGQGRN +GPVTPLREM 
Sbjct: 181 PPPRPYENGYGEYRGERENGNYGGMRRSRSFGDVGESMEREGQGQGRN-KGPVTPLREMP 240

Query: 241 TERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300
            ERIFGKMGHLQRLLDRFLSCRPTGLAK+SRMILYALYPLVRESFQLYADICEVLAVLLD
Sbjct: 241 IERIFGKMGHLQRLLDRFLSCRPTGLAKSSRMILYALYPLVRESFQLYADICEVLAVLLD 300

Query: 301 KFFDMEYSDCMKAFDAYGSAAKQIDELISLYNWCKDTGVARSSEYPEVQRITSKLLETLE 360
           KFFDMEYSD MKAFDAYGSAAKQIDELI+ YNWCKDTGVARSSEYPEVQRITSKLLETLE
Sbjct: 301 KFFDMEYSDSMKAFDAYGSAAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE 360

Query: 361 EFLRERGKKPKSPEREPPPPVAQEEEPVPDMNEIKALPPPENYTPPPP-PEPEPAPKPQQ 420
           EFLRERGK PKSPEREPPPP  QEEEPVPDMNEIKALPPPENYTPPPP PEP+PAPKPQ 
Sbjct: 361 EFLRERGKAPKSPEREPPPPAPQEEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPKPQP 420

Query: 421 QVSEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480
           QV+EDLVNLRDDAVSAD QGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA
Sbjct: 421 QVTEDLVNLRDDAVSADAQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480

Query: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV 540
           EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLS GSASSV
Sbjct: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSSGSASSV 540

Query: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLTIPPPSYVQMVEMEKKQQLLVQEQQV 600
           ALPGPGNSKTPVLALPAPDGTVQA+NQDPFAASL+IPPPSYVQMVEMEKKQ LLVQEQQ+
Sbjct: 541 ALPGPGNSKTPVLALPAPDGTVQALNQDPFAASLSIPPPSYVQMVEMEKKQHLLVQEQQL 600

Query: 601 WQQYSRDGMQGQSSLNKISNPPGYYT--AGMMAPMPYGMPPANGMGGYYYIPQ 650
           WQQY+RDGMQGQSSLNK+SNPPGYY   AG MAPMPYGMPP NG G YYYIPQ
Sbjct: 601 WQQYARDGMQGQSSLNKMSNPPGYYNTAAGPMAPMPYGMPPMNGSG-YYYIPQ 651

BLAST of MC11g0454 vs. ExPASy TrEMBL
Match: A0A6J1F990 (probable clathrin assembly protein At4g32285 OS=Cucurbita moschata OX=3662 GN=LOC111443479 PE=4 SV=1)

HSP 1 Score: 1171 bits (3030), Expect = 0.0
Identity = 607/653 (92.96%), Postives = 626/653 (95.87%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTS 60
           MAPSTIRKAIGAVKDQTSIGIAKVASN APDLEVAIVKATSHDDDPASEKYIREIL+LTS
Sbjct: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNTAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60

Query: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120
           YSRGYV+ACV AISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN
Sbjct: 61  YSRGYVNACVVAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120

Query: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSTRGNSRGDDRFEGRDDFRS 180
           MSDFKDEAHSSSWDHSAF+RTYAFYLDQRLEL+LFEKKGGS RGNSRGDDRF+GRDDFRS
Sbjct: 121 MSDFKDEAHSSSWDHSAFIRTYAFYLDQRLELVLFEKKGGSARGNSRGDDRFDGRDDFRS 180

Query: 181 PPPRPYDNGYGEYRGEREYGNYGEMRRSRSYGDVGESVGREGQGQGRNNRGPVTPLREMV 240
           PPPRPYDNGYGEYRGEREYGNYG MRRSRS+GDVGES+ REGQG+   N+GPVTPLREM 
Sbjct: 181 PPPRPYDNGYGEYRGEREYGNYGGMRRSRSFGDVGESMEREGQGR---NKGPVTPLREMP 240

Query: 241 TERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300
            ERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD
Sbjct: 241 IERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300

Query: 301 KFFDMEYSDCMKAFDAYGSAAKQIDELISLYNWCKDTGVARSSEYPEVQRITSKLLETLE 360
           KFFDMEYSD MKAFDAYGSAAKQIDELI+ YNWCKDTGVARSSEYPEVQRITSKLLETLE
Sbjct: 301 KFFDMEYSDSMKAFDAYGSAAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE 360

Query: 361 EFLRERGKKPKSPEREPPPPVAQEEEPVPDMNEIKALPPPENYTPPPP-PEPEPAPKPQQ 420
           EFLRERGK PKSPEREPPPP  QEEEPVPDMNEIKALPPPENYTPPPP PEP+PAP PQ 
Sbjct: 361 EFLRERGKAPKSPEREPPPPAPQEEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPIPQP 420

Query: 421 QVSEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480
           QV+EDLVNLRDDAVSAD QGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA
Sbjct: 421 QVTEDLVNLRDDAVSADAQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480

Query: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV 540
           EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHT+TAQLSGGSASSV
Sbjct: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTNTAQLSGGSASSV 540

Query: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLTIPPPSYVQMVEMEKKQQLLVQEQQV 600
           ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASL++PPPSYVQMVEMEKKQ +LVQEQQ+
Sbjct: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHMLVQEQQL 600

Query: 601 WQQYSRDGMQGQSSLNKISNPPGYYT--AGMMAPMPYGMPPANGMGGYYYIPQ 650
           WQQY+RDGMQGQSSLNK+SNPPGYY   AG MAPMPYGMPP NG G YYYIPQ
Sbjct: 601 WQQYARDGMQGQSSLNKMSNPPGYYNTAAGPMAPMPYGMPPMNGSG-YYYIPQ 649

BLAST of MC11g0454 vs. ExPASy TrEMBL
Match: A0A6J1FFH4 (probable clathrin assembly protein At4g32285 OS=Cucurbita moschata OX=3662 GN=LOC111443478 PE=4 SV=1)

HSP 1 Score: 1169 bits (3023), Expect = 0.0
Identity = 605/653 (92.65%), Postives = 625/653 (95.71%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTS 60
           MAPSTIRKAIGAVKDQTSIGIAKVASN APDLEVAIVKATSHDDDPASEKYIREIL+LTS
Sbjct: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNTAPDLEVAIVKATSHDDDPASEKYIREILSLTS 60

Query: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120
           YSRGYVSACV A+SKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN
Sbjct: 61  YSRGYVSACVVAVSKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120

Query: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSTRGNSRGDDRFEGRDDFRS 180
           MSDFKDEAHSSSWDHSAF+RTYAFYLDQRLEL+LFEKKG S +GNSRGDDRF+GRDDFRS
Sbjct: 121 MSDFKDEAHSSSWDHSAFIRTYAFYLDQRLELVLFEKKGSSAKGNSRGDDRFDGRDDFRS 180

Query: 181 PPPRPYDNGYGEYRGEREYGNYGEMRRSRSYGDVGESVGREGQGQGRNNRGPVTPLREMV 240
           PPPRPYDNGYGEYRGEREYGNYG MRRSRS+GDVGES+ REGQG+   N+GPVTPLREM 
Sbjct: 181 PPPRPYDNGYGEYRGEREYGNYGGMRRSRSFGDVGESMEREGQGR---NKGPVTPLREMP 240

Query: 241 TERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300
            ERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD
Sbjct: 241 IERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVLLD 300

Query: 301 KFFDMEYSDCMKAFDAYGSAAKQIDELISLYNWCKDTGVARSSEYPEVQRITSKLLETLE 360
           KFFDMEYSD MKAFDAYGSAAKQIDELI+ YNWCKDTGVARSSEYPEVQRITSKLLETLE
Sbjct: 301 KFFDMEYSDSMKAFDAYGSAAKQIDELIAFYNWCKDTGVARSSEYPEVQRITSKLLETLE 360

Query: 361 EFLRERGKKPKSPEREPPPPVAQEEEPVPDMNEIKALPPPENYTPPPP-PEPEPAPKPQQ 420
           EFLRERGK PKSPEREPPPP  QEEEPVPDMNEIKALPPPENYTPPPP PEP+PAP PQ 
Sbjct: 361 EFLRERGKAPKSPEREPPPPAPQEEEPVPDMNEIKALPPPENYTPPPPEPEPQPAPIPQP 420

Query: 421 QVSEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480
           QV+EDLVNLRDDAVSAD QGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA
Sbjct: 421 QVTEDLVNLRDDAVSADAQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAWQTPAA 480

Query: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGGSASSV 540
           EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHT+TAQLSGGSASSV
Sbjct: 481 EPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTNTAQLSGGSASSV 540

Query: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLTIPPPSYVQMVEMEKKQQLLVQEQQV 600
           ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASL++PPPSYVQMVEMEKKQ +LVQEQQ+
Sbjct: 541 ALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLSVPPPSYVQMVEMEKKQHMLVQEQQL 600

Query: 601 WQQYSRDGMQGQSSLNKISNPPGYYT--AGMMAPMPYGMPPANGMGGYYYIPQ 650
           WQQY+RDGMQGQSSLNK+SNPPGYY   AG MAPMPYGMPP NG G YYYIPQ
Sbjct: 601 WQQYARDGMQGQSSLNKMSNPPGYYNMAAGPMAPMPYGMPPMNGSG-YYYIPQ 649

BLAST of MC11g0454 vs. TAIR 10
Match: AT2G25430.1 (epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly protein-related )

HSP 1 Score: 930.2 bits (2403), Expect = 8.8e-271
Identity = 511/668 (76.50%), Postives = 562/668 (84.13%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTS 60
           MAPS IRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTS
Sbjct: 1   MAPS-IRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTS 60

Query: 61  YSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLN 120
            SRGY+ ACV+++S+RL+KTRDW+VALKAL+LVHRLLNEGDP+FQEEILY+TRRGTR+LN
Sbjct: 61  LSRGYILACVTSVSRRLSKTRDWVVALKALMLVHRLLNEGDPIFQEEILYSTRRGTRMLN 120

Query: 121 MSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKK-------GGSTRGNSRGDDRF- 180
           MSDF+DEAHSSSWDHSAFVRTYA YLDQRLEL LFE+K       GG++  +S  DDR+ 
Sbjct: 121 MSDFRDEAHSSSWDHSAFVRTYAGYLDQRLELALFERKSGVSVNSGGNSSHHSNNDDRYG 180

Query: 181 EGRDDFRSPPPRPYD--NGYG---EYRGEREYGNYGEMRRSRSYGDVGESVGREGQGQGR 240
            GRDDFRSPPPR YD  NG G   ++RG+   G  G  +RSRSYGD+ E +G  G G GR
Sbjct: 181 RGRDDFRSPPPRSYDYENGGGGGSDFRGDNN-GYGGVPKRSRSYGDMTE-MGGGGGGGGR 240

Query: 241 NNRGPVTPLREMVTERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQL 300
           + +  VTPLREM  ERIFGKMGHLQRLLDRFLS RPTGLAKNSRMIL ALYP+VRESF+L
Sbjct: 241 DEKKVVTPLREMTPERIFGKMGHLQRLLDRFLSLRPTGLAKNSRMILIALYPVVRESFKL 300

Query: 301 YADICEVLAVLLDKFFDMEYSDCMKAFDAYGSAAKQIDELISLYNWCKDTGVARSSEYPE 360
           YADICEVLAVLLDKFFDMEYSDC+KAFDAY SAAKQIDELI+ YNWCK+TGVARSSEYPE
Sbjct: 301 YADICEVLAVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGVARSSEYPE 360

Query: 361 VQRITSKLLETLEEFLRERGKKPKSPER---EPPPPVAQEEEPVPDMNEIKALPPPENYT 420
           VQRITSKLLETLEEF+R+R K+ KSPER   E PPPV +EEEP PDMNEIKALPPPENYT
Sbjct: 361 VQRITSKLLETLEEFVRDRAKRGKSPERKEIEAPPPVVEEEEPEPDMNEIKALPPPENYT 420

Query: 421 PPPPPEPEPAPKPQQQVSEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPS 480
           PPPPPEPEP P+ + Q +EDLVNLR+D V+ADDQGNK ALALFAGP  N  NG WEAF S
Sbjct: 421 PPPPPEPEPQPE-KPQFTEDLVNLREDEVTADDQGNKFALALFAGPPGN--NGKWEAFSS 480

Query: 481 DGQPEVTSAWQTPAAEPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQ 540
           +G   VTSAWQ PAAEPGKADWELALVET SNL +Q AALGGG D LLLNGMYDQGMVRQ
Sbjct: 481 NG---VTSAWQNPAAEPGKADWELALVETTSNLEKQTAALGGGFDNLLLNGMYDQGMVRQ 540

Query: 541 HTSTAQLSGGSASSVALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLTIPPPSYVQMV 600
           H ST+QL+GGSASSVALP PG +   VLALPAPDGTV+ VNQDPFAASLTIPPPSYVQM 
Sbjct: 541 HVSTSQLTGGSASSVALPLPGKTNNQVLALPAPDGTVEKVNQDPFAASLTIPPPSYVQMA 600

Query: 601 EMEKKQQLLVQEQQVWQQYSRDGMQGQSSLNKISNPPGYYTAGMMAPMP-YGMPPANGMG 648
           EMEKKQ LL QEQQ+WQQY RDGM+GQ+SL K++            P+P YGMPP NGMG
Sbjct: 601 EMEKKQYLLSQEQQLWQQYQRDGMRGQASLAKMNT----------GPVPAYGMPPVNGMG 649

BLAST of MC11g0454 vs. TAIR 10
Match: AT4G32285.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 891.0 bits (2301), Expect = 5.9e-259
Identity = 485/657 (73.82%), Postives = 538/657 (81.89%), Query Frame = 0

Query: 5   TIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTSYSRG 64
           ++RKAIG VKDQTSIGIAKVASNMAPDLEVAIVKATSHDDD +S+KYIREIL+LTS SRG
Sbjct: 4   SMRKAIGVVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDQSSDKYIREILSLTSLSRG 63

Query: 65  YVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLNMSDF 124
           YV ACV+++S+RL KTRDWIVALKAL+LVHRLLNEGDP+FQEEILYATRRGTR+LNMSDF
Sbjct: 64  YVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRILNMSDF 123

Query: 125 KDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKK-----GGSTRGNSRGDDRF-EGRDDF 184
           +DEAHSSSWDHSAFVRTYA YLDQRLEL LFE++     G S+   S GDD +   RDDF
Sbjct: 124 RDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDGYNRSRDDF 183

Query: 185 RSPPPRPYDNGYGEYRGEREYGNYGEMRRSRSYGDVGESVGREGQGQGRNNRGPVTPLRE 244
           RSPPPR YD   G          +G  +RSRS+GDV E   RE        +  VTPLRE
Sbjct: 184 RSPPPRTYDYETG--------NGFGMPKRSRSFGDVNEIGARE-------EKKSVTPLRE 243

Query: 245 MVTERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVL 304
           M  ERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMIL A+YP+V+ESF+LYADICEVLAVL
Sbjct: 244 MTPERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVL 303

Query: 305 LDKFFDMEYSDCMKAFDAYGSAAKQIDELISLYNWCKDTGVARSSEYPEVQRITSKLLET 364
           LDKFFDMEY+DC+KAFDAY SAAKQIDELI+ Y+WCKDTGVARSSEYPEVQRITSKLLET
Sbjct: 304 LDKFFDMEYTDCVKAFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLET 363

Query: 365 LEEFLRERGKKPKSPERE----PPPPVAQEEEPVPDMNEIKALPPPENYTPPPPPEPEPA 424
           LEEF+R+R K+ KSPER+    PP P    EEPV DMNEIKALPPPEN+TPPPPP PEP 
Sbjct: 364 LEEFVRDRAKRAKSPERKEIEAPPAPAPPVEEPV-DMNEIKALPPPENHTPPPPPAPEPK 423

Query: 425 PKPQQQVSEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAW 484
           P+ Q QV++DLVNLR+D VS DDQGNK ALALFAGP AN  NG WEAF SD    VTSAW
Sbjct: 424 PQ-QPQVTDDLVNLREDDVSGDDQGNKFALALFAGPPAN--NGKWEAFSSDN--NVTSAW 483

Query: 485 QTPAAEPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGG 544
           Q PAAE GKADWELALVETASNL  QKAA+GGGLDPLLLNGMYDQG VRQH ST++L+GG
Sbjct: 484 QNPAAELGKADWELALVETASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQHVSTSELTGG 543

Query: 545 SASSVALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLTIPPPSYVQMVEMEKKQQLLV 604
           S+SSVALP PG   + +LALPAPDGTVQ VNQDPFAASLTIPPPSYVQM EM+KKQ LL 
Sbjct: 544 SSSSVALPLPGKVNSHILALPAPDGTVQKVNQDPFAASLTIPPPSYVQMAEMDKKQYLLT 603

Query: 605 QEQQVWQQYSRDGMQGQSSLNKISNPPGYYTAGMMAPMPYGMPPANGMG----GYYY 648
           QEQQ+WQQY ++GM+GQ+SL K++             MPYGMPP NGMG    GYYY
Sbjct: 604 QEQQLWQQYQQEGMRGQASLAKMNT--------AQTAMPYGMPPVNGMGPSPMGYYY 631

BLAST of MC11g0454 vs. TAIR 10
Match: AT4G32285.2 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 891.0 bits (2301), Expect = 5.9e-259
Identity = 485/657 (73.82%), Postives = 538/657 (81.89%), Query Frame = 0

Query: 5   TIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTSYSRG 64
           ++RKAIG VKDQTSIGIAKVASNMAPDLEVAIVKATSHDDD +S+KYIREIL+LTS SRG
Sbjct: 4   SMRKAIGVVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDQSSDKYIREILSLTSLSRG 63

Query: 65  YVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRLLNMSDF 124
           YV ACV+++S+RL KTRDWIVALKAL+LVHRLLNEGDP+FQEEILYATRRGTR+LNMSDF
Sbjct: 64  YVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRILNMSDF 123

Query: 125 KDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKK-----GGSTRGNSRGDDRF-EGRDDF 184
           +DEAHSSSWDHSAFVRTYA YLDQRLEL LFE++     G S+   S GDD +   RDDF
Sbjct: 124 RDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDGYNRSRDDF 183

Query: 185 RSPPPRPYDNGYGEYRGEREYGNYGEMRRSRSYGDVGESVGREGQGQGRNNRGPVTPLRE 244
           RSPPPR YD   G          +G  +RSRS+GDV E   RE        +  VTPLRE
Sbjct: 184 RSPPPRTYDYETG--------NGFGMPKRSRSFGDVNEIGARE-------EKKSVTPLRE 243

Query: 245 MVTERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVL 304
           M  ERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMIL A+YP+V+ESF+LYADICEVLAVL
Sbjct: 244 MTPERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVL 303

Query: 305 LDKFFDMEYSDCMKAFDAYGSAAKQIDELISLYNWCKDTGVARSSEYPEVQRITSKLLET 364
           LDKFFDMEY+DC+KAFDAY SAAKQIDELI+ Y+WCKDTGVARSSEYPEVQRITSKLLET
Sbjct: 304 LDKFFDMEYTDCVKAFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLET 363

Query: 365 LEEFLRERGKKPKSPERE----PPPPVAQEEEPVPDMNEIKALPPPENYTPPPPPEPEPA 424
           LEEF+R+R K+ KSPER+    PP P    EEPV DMNEIKALPPPEN+TPPPPP PEP 
Sbjct: 364 LEEFVRDRAKRAKSPERKEIEAPPAPAPPVEEPV-DMNEIKALPPPENHTPPPPPAPEPK 423

Query: 425 PKPQQQVSEDLVNLRDDAVSADDQGNKLALALFAGPAANGANGSWEAFPSDGQPEVTSAW 484
           P+ Q QV++DLVNLR+D VS DDQGNK ALALFAGP AN  NG WEAF SD    VTSAW
Sbjct: 424 PQ-QPQVTDDLVNLREDDVSGDDQGNKFALALFAGPPAN--NGKWEAFSSDN--NVTSAW 483

Query: 485 QTPAAEPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMYDQGMVRQHTSTAQLSGG 544
           Q PAAE GKADWELALVETASNL  QKAA+GGGLDPLLLNGMYDQG VRQH ST++L+GG
Sbjct: 484 QNPAAELGKADWELALVETASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQHVSTSELTGG 543

Query: 545 SASSVALPGPGNSKTPVLALPAPDGTVQAVNQDPFAASLTIPPPSYVQMVEMEKKQQLLV 604
           S+SSVALP PG   + +LALPAPDGTVQ VNQDPFAASLTIPPPSYVQM EM+KKQ LL 
Sbjct: 544 SSSSVALPLPGKVNSHILALPAPDGTVQKVNQDPFAASLTIPPPSYVQMAEMDKKQYLLT 603

Query: 605 QEQQVWQQYSRDGMQGQSSLNKISNPPGYYTAGMMAPMPYGMPPANGMG----GYYY 648
           QEQQ+WQQY ++GM+GQ+SL K++             MPYGMPP NGMG    GYYY
Sbjct: 604 QEQQLWQQYQQEGMRGQASLAKMNT--------AQTAMPYGMPPVNGMGPSPMGYYY 631

BLAST of MC11g0454 vs. TAIR 10
Match: AT1G03050.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 477.6 bits (1228), Expect = 1.6e-134
Identity = 297/663 (44.80%), Postives = 398/663 (60.03%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVASNMA--PDLEVAIVKATSHDDDPASEKYIREILNL 60
           M  S  ++AIGAVKDQTS+G+AKV    A   +L+VAIVKAT H++ PA EKYIREIL+L
Sbjct: 1   MGSSKFKRAIGAVKDQTSVGLAKVNGRSASLSELDVAIVKATRHEEFPAEEKYIREILSL 60

Query: 61  TSYSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRL 120
           TSYSR Y++ACVS +S+RL KT+ W VALK LIL+ RLL EGD  +++EI +ATRRGTRL
Sbjct: 61  TSYSRSYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGDQAYEQEIFFATRRGTRL 120

Query: 121 LNMSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSTRGNSRGDDRFEGRDDF 180
           LNMSDF+D + S+SWD+SAFVRTYA YLD+RL+  +        R   RG     G  D 
Sbjct: 121 LNMSDFRDVSRSNSWDYSAFVRTYALYLDERLDFRM------QARHGKRGVYCVGGEAD- 180

Query: 181 RSPPPRPYDNGYGEYRGEREYGNYGEMRRSRSYGDVGESVGREGQGQGRNNRGPVTPLRE 240
                                    E  + ++  D+  ++    Q           P+ E
Sbjct: 181 -------------------------EEEQDQAAADLSTAIVVRSQ-----------PIAE 240

Query: 241 MVTERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVL 300
           M TE+IF ++ HLQ+LLDRFL+CRPTG A+N+R+++ ALYP+V+ESFQ+Y D+ E++ +L
Sbjct: 241 MKTEQIFIRIQHLQQLLDRFLACRPTGNARNNRVVIVALYPIVKESFQIYYDVTEIMGIL 300

Query: 301 LDKFFDMEYSDCMKAFDAYGSAAKQIDELISLYNWCKDTGVARSSEYPEVQRITSKLLET 360
           +++F +++  D +K +D +   +KQ +EL   Y+WCK+ G+ARSSEYPE+++IT K L+ 
Sbjct: 301 IERFMELDIPDSIKVYDIFCRVSKQFEELDQFYSWCKNMGIARSSEYPEIEKITQKKLDL 360

Query: 361 LEEFLRER--------GKKPKSPEREPPPPVAQEE--EPVPDMNEIKALPPPENYTPPPP 420
           ++EF+R++         K  KS   E       EE  E   DMN IKAL       P PP
Sbjct: 361 MDEFIRDKSALEHTKQSKSVKSEADEDDDEARTEEVNEEQEDMNAIKAL-------PEPP 420

Query: 421 PEPEPAPKPQQQVSE------------DLVNL-RDDAVSADDQGNKLALALFAGPAANGA 480
           P+ E   KP+++  E            DL++L   +   A   G+ LALALF GP A+G+
Sbjct: 421 PKEEDDVKPEEEAKEEVIIEKKQEEMGDLLDLGNTNGGEAGQAGDSLALALFDGPYASGS 480

Query: 481 NG----SWEAFPSDGQPEVTSAWQTPAAEPGKADWELALVETASNLSRQKAALGGGLDPL 540
                  WEAF  D                  ADWE ALV+TA+NLS QK+ LGGG D L
Sbjct: 481 GSESGPGWEAFKDD-----------------SADWETALVQTATNLSGQKSELGGGFDML 540

Query: 541 LLNGMYDQGMVRQ--HTSTAQLSGGSASSVALPGPGNSKTPVLALPAPD------GTVQA 600
           LLNGMY  G V     TSTA  + GSASS+A    G     +LALPAP       G + +
Sbjct: 541 LLNGMYQHGAVNAAVKTSTAYGASGSASSMAFGSAGRPAATMLALPAPSTANGNAGNINS 596

Query: 601 -VNQDPFAASLTIPPPSYVQMVEMEKKQQLLVQEQQVWQQYSRDGMQGQSSLNKISNPPG 626
            V  DPFAASL + PP+YVQM +MEKKQ++L++EQ +W QYSRDG QG  +L +  N P 
Sbjct: 601 PVPMDPFAASLEVAPPAYVQMNDMEKKQRMLMEEQMMWDQYSRDGRQGHMNLRQNQNQPY 596

BLAST of MC11g0454 vs. TAIR 10
Match: AT4G02650.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 463.4 bits (1191), Expect = 3.0e-130
Identity = 293/664 (44.13%), Postives = 390/664 (58.73%), Query Frame = 0

Query: 1   MAPSTIRKAIGAVKDQTSIGIAKVA--SNMAPDLEVAIVKATSHDDDPASEKYIREILNL 60
           M  S +++AIGAVKDQTS+G+AKV   S+   +LE+A+VKAT HDD PA +KYIREIL L
Sbjct: 1   MGSSKLKRAIGAVKDQTSVGLAKVGGRSSSLTELEIAVVKATRHDDYPAEDKYIREILCL 60

Query: 61  TSYSRGYVSACVSAISKRLAKTRDWIVALKALILVHRLLNEGDPVFQEEILYATRRGTRL 120
           TSYSR YVSACV+ +S+RL KT++W VALK LIL+ RLL +GD  +++EI +ATRRGTRL
Sbjct: 61  TSYSRNYVSACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEIFFATRRGTRL 120

Query: 121 LNMSDFKDEAHSSSWDHSAFVRTYAFYLDQRLELMLFEKKGGSTRGNSRGDDRFEGRDDF 180
           LNMSDF+D + S SWD+SAFVRTYA YLD+RL+  +  ++G    G   G D   G +D 
Sbjct: 121 LNMSDFRDASQSDSWDYSAFVRTYALYLDERLDYRMQGRRGKKKSGGGGGGDGDSGEED- 180

Query: 181 RSPPPRPYDNGYGEYRGEREYGNYGEMRRSRSYGDVGESVGREGQGQGRNNRGPVTPLRE 240
                                 + G     RS   V +S                 P+ E
Sbjct: 181 ---------------------DHRGTSNDIRSKAIVVKS----------------KPVAE 240

Query: 241 MVTERIFGKMGHLQRLLDRFLSCRPTGLAKNSRMILYALYPLVRESFQLYADICEVLAVL 300
           M TE+IF ++ HLQ+LLDRFL+CRPTG AKN+R+++ A+YP+V+ESFQLY +I E++ VL
Sbjct: 241 MKTEKIFNRVQHLQQLLDRFLACRPTGNAKNNRVVIVAMYPIVKESFQLYYNITEIMGVL 300

Query: 301 LDKFFDMEYSDCMKAFDAYGSAAKQIDELISLYNWCKDTGVARSSEYPEVQRITSKLLET 360
           +++F +++  D +K ++ +   +KQ DEL   Y WCK+  VARSSEYPE+++IT K L+ 
Sbjct: 301 IERFMELDIHDSIKVYEIFCRVSKQFDELDPFYGWCKNMAVARSSEYPELEKITQKKLDL 360

Query: 361 LEEFLRERG------------KKPKSPEREPPPPVAQEEEPVPDMNEIKALPPPENYTPP 420
           ++EF+R++             +  KS E E      QE +   D+N IKALP PE     
Sbjct: 361 MDEFIRDKSALAAQTTKSSSKRSNKSEEEESKTEYIQENQ--EDLNSIKALPAPEQKEEE 420

Query: 421 PPPEPEPAPKPQQQV------SEDLVNLRDDA-VSADDQGNKLALALFAGPAA--NGANG 480
              E     K  ++V        DL++L D+A V+A   G+ LALALF G     + +  
Sbjct: 421 EEEEKMETKKDVEEVVSRQDQEGDLLDLTDEAGVTAGTVGDSLALALFDGVVGTESASGP 480

Query: 481 SWEAFPSDGQPEVTSAWQTPAAEPGKADWELALVETASNLSRQKAALGGGLDPLLLNGMY 540
            WEAF                     ADWE  LV +A+ LS QK+ LGGG D LLL+GMY
Sbjct: 481 GWEAF-----------------NDNSADWETDLVRSATRLSGQKSELGGGFDTLLLDGMY 540

Query: 541 DQGMVRQ--HTSTAQLSGGSASSVALPGPGNSKTPVLALPAPDGTVQA-----VNQDPFA 600
             G V     TSTA  S GSASSVA    G+    +LALPAP  T        V  DPFA
Sbjct: 541 QYGAVNAAVKTSTAYGSSGSASSVAFGSAGSPAASMLALPAPPPTANGNRNSPVMVDPFA 600

Query: 601 ASLTIPPPSYVQMVEMEKKQQLLVQEQQVWQQYSRDGMQGQSSLNKISNPPGYYTAGMMA 635
           ASL + PP+YVQM +MEKKQ+LL++EQ +W QY+R G QG  +  +      Y     M 
Sbjct: 601 ASLEVAPPAYVQMNDMEKKQRLLMEEQIMWDQYNRSGRQGHMNFGQNQQQQYYQLPYSMG 607

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LF201.2e-26976.50Putative clathrin assembly protein At2g25430 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8S9J88.3e-25873.82Probable clathrin assembly protein At4g32285 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9SA652.2e-13344.80Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8GX474.3e-12944.13Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9ZVN68.8e-6637.08Clathrin coat assembly protein AP180 OS=Arabidopsis thaliana OX=3702 GN=AP180 PE... [more]
Match NameE-valueIdentityDescription
XP_008466416.10.094.33PREDICTED: putative clathrin assembly protein At2g25430 [Cucumis melo][more]
XP_038898099.10.094.33putative clathrin assembly protein At2g25430 [Benincasa hispida][more]
XP_004136352.10.093.72putative clathrin assembly protein At2g25430 [Cucumis sativus] >KGN60096.1 hypot... [more]
KAG6591568.10.093.42putative clathrin assembly protein, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022976123.10.093.26putative clathrin assembly protein At2g25430 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A1S3CRD10.094.33putative clathrin assembly protein At2g25430 OS=Cucumis melo OX=3656 GN=LOC10350... [more]
A0A0A0LJA40.093.72ENTH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G877670 PE=4 S... [more]
A0A6J1IL730.093.26putative clathrin assembly protein At2g25430 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1F9900.092.96probable clathrin assembly protein At4g32285 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1FFH40.092.65probable clathrin assembly protein At4g32285 OS=Cucurbita moschata OX=3662 GN=LO... [more]
Match NameE-valueIdentityDescription
AT2G25430.18.8e-27176.50epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly p... [more]
AT4G32285.15.9e-25973.82ENTH/ANTH/VHS superfamily protein [more]
AT4G32285.25.9e-25973.82ENTH/ANTH/VHS superfamily protein [more]
AT1G03050.11.6e-13444.80ENTH/ANTH/VHS superfamily protein [more]
AT4G02650.13.0e-13044.13ENTH/ANTH/VHS superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013809ENTH domainSMARTSM00273enth_2coord: 30..160
e-value: 8.4E-49
score: 178.1
IPR013809ENTH domainPROSITEPS50942ENTHcoord: 24..160
score: 34.460052
IPR014712ANTH domain superfamilyGENE3D1.20.58.150ANTH domaincoord: 227..374
e-value: 5.0E-41
score: 141.8
IPR011417AP180 N-terminal homology (ANTH) domainPFAMPF07651ANTHcoord: 31..365
e-value: 5.0E-83
score: 278.3
IPR008942ENTH/VHSGENE3D1.25.40.90coord: 2..155
e-value: 7.6E-44
score: 151.0
IPR008942ENTH/VHSSUPERFAMILY48464ENTH/VHS domaincoord: 30..151
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 364..422
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 210..233
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 399..416
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 160..182
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 364..381
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 160..193
NoneNo IPR availablePANTHERPTHR22951:SF84SUBFAMILY NOT NAMEDcoord: 5..628
NoneNo IPR availablePANTHERPTHR22951CLATHRIN ASSEMBLY PROTEINcoord: 5..628
NoneNo IPR availableCDDcd16987ANTH_N_AP180_plantcoord: 32..152
e-value: 3.52907E-68
score: 216.336
NoneNo IPR availableSUPERFAMILY89009GAT-like domaincoord: 235..365

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC11g0454.1MC11g0454.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0048268 clathrin coat assembly
biological_process GO:0072583 clathrin-dependent endocytosis
biological_process GO:0006900 vesicle budding from membrane
cellular_component GO:0005905 clathrin-coated pit
cellular_component GO:0030136 clathrin-coated vesicle
cellular_component GO:0005794 Golgi apparatus
molecular_function GO:0005545 1-phosphatidylinositol binding
molecular_function GO:0032050 clathrin heavy chain binding
molecular_function GO:0005546 phosphatidylinositol-4,5-bisphosphate binding
molecular_function GO:0000149 SNARE binding
molecular_function GO:0030276 clathrin binding
molecular_function GO:0005543 phospholipid binding