MC11g0385 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC11g0385
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Aspartyl protease family protein
Location	MC11: 3052757 .. 3056984 (-)
RNA-Seq Expression	MC11g0385
Synteny	MC11g0385

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GTAAAACCATTGGAAAATCCACAACTGTACTGTGGAGTGGAGCATCGTCCTTTTATTTAAAAAGGGCTTAAGGGTACTTCTATCAACAACCCAAAAAAGAAAAAAGGAAAAAAAAAGCTCAATTTTCTCAGAGCTCTTCTGCAGCTCTAATCCATGGCGGCTCTCTCTCTCTCCTCTTTCTCACTCCTCTTCTTTTTCCTTTTCTTCCACTCGTTCGCCGGAAAGGTCGCGCCGGACGGCCACCACCTCACTGTCGAACTCGCCGCCCTTCTGCCCTCCGCCGCCTGCAGCCGCCGCTCTGCCAAAGGTCTCTTTCTCTCTTACTTTTGCTAAATTTCAGTGTGAATATTCTGAATTTTGACCCCCCCTTTTTTAATGGTGAGATGGGAAACGAAAGTAATATTATATTATATTTTAATTATGGTAAAAAATTGATTTTACGTTAAACGAGGAAGATGAATAATTTTATTGGAAAATGGTACTTTGTTTCTTGTGAAGTTTTAAATTGGGGAAGAAGATAATCAAGAATGTAATTTTCTTTTTTTTTTTTTGGATGTAGTTGTTATACAATAAGCACGAACTATCATTAATCTCTCTGCCAAAAACGTGCTTACGTGTCATAAATATAGCCATGGCTATTTTTTTCCTTAGTTCAGCGATGGCTATTTCTTTATTACGACCTTAAATTTATAGTTTTACGATTCATTGAATATATATATATATATTTTTATTATTATTAGTTGTGGAAAAGTTTTAAAAAATCACCCAAATTAAAGGGGAAAAAAAAGACCAAATGGGTCTGTCTGTCTGTCTCTGAGCTTTATCTTCGTCAAATTTGAAGAATATGTTATCGGTGGGTTGTTGGTTTGTGGCTGAAAAGCAAAGGAGATTTTTTAAAGAGTCAATAAATGGGTCCTCAATTGAAATACTGAAGTGACTAATTACCATATAATTAACACATTATTTTAATATGATGACAAATTGTTTATGTTGGATTAAAAATTTTAATTACCCTAATTTTGATAATGGTTTCATTTTCTATTTACTAAAATTATATTTACCTACTAACACTTTCTTTATTTGTAATGTACTTCACGAAAAGATTTTTAAGATCTTGACCCAGATTTTAAAAGTTAGAAAGCAATTCATTTATTTTATTTTTTATGGAATCTTTCTTGAAATCTTGCTAAAGATTTTAAGCGTTCTTAATCTACAAGATAAGCCTAAATTATTATTATTTTTAAATCATTATCAGTTGGGTCAATTTTAATATAACGGGAAGTGCTACGGAAAGAATAATTACTAAAATATTGCTTATATTTAGAATTGTTTATAATTGGAAAATGATTCTTTTTTCATTTTTATTTTTATTTTTTTATTTTTTTGAACATGGAAAAATGATTCTTACTCATAGTCAAAAAGTACAAAAAGACAAACAAGTACACCGTCAGAGGTTGCAAATATTCCGAAACCTCATACGGCTGTGATATTAATTGTCAAAAATCATAAATGTAATTAATTCAGCTCCCAACTGAAGCCTAAAGCCGCCGCTATAAATGCGTTTTCAAGACCATCAGTCCCCTTCAAGTGAGCCTTCCAGAGGCCCATGGTTAAGACAATTCCTATTATTTGGCCCATTAAAAGAGCCCACCTTATACTTAATCTAGTACGTAGCCCATTTGTCAGCCCAACTTCGAATCATATTTACTAACTTTTTCCTTATGGAAACAAAATAAAACAAAAAACCAAAATTAATTTAACATAATTCTATCCCATTCTTATAGTACAACAAAGTGGAGGTGAGAGACTCGAACTCATGATTTATTGGTCAAAGACAGGTGTTAATTACCGCTAAGTTAAGTTCATATTGACCATATAATTATGTTGATTAAGCATACTCTAATTAGAGTTAAGACATTAATAGCTTTATTTTAAGTTATAGATTAGAAACACCTCGTCATATTCTTCTTTTTTTTTAGCTATAAAAGTTGATATAATTATTCATTTGATAGGAGACTAACAATGCTTTTTCCCATTACAATATGTTCAAGTTTTGATTACACCTCTTTTTTTTTAAGATCGTGATTATTATTAGATTAAAATACTAAAAAAAGTCAACATGAACCTAGTTTAGTTGTAATTTACATTTACTCCTGCGGTTTTGTATCTTGTGATCTACAAATTGTTATACTGAAAAAAAGTTAAAAATTCTCTCGAAATTGACTGGGTTGTGTTGTGGTGGGCCGGTATCCCAAGTTACAGGTGTGAAAAAAGAAGATTCGTCTCCGTCTCTGGAGGTGGTTCACCGGCATGGGCCGTGCGCCGGAGCTTCAAAAGCGCCGAGCGAAGCGGAAATCTTTGTCCGCGATCAGGCTCGGGTGGACCTCATCCACTCCAAGTTCTCAGAGGAGTCTGGTTCGGGTTCGGTCCAGCGTATACGGCCGTCGAAGGCGACGAAGATTCCGGCGAAGTCCGGCGCCACCATCGGGTCCGGTAATTACATAGTGAGCGTCGGATTGGGGACGCCGAAGAAGGAGCTGTCGCTAATATTCGACACGGGGAGCGATCTGACGTGGACTCAGTGCGAGCCTTGCGCCAGATATTGCTACAGCCAGAAGGATCCGGTTTTCGCTCCTTCCAAATCCACCTCTTATTCCAATATCTCTTGTTCCTCCCCTTCCTGCTCTCAGCTTGAATCTGGCACCGGTAAGTAATTCTAATTTCTACCTTTTTACTTCCTCCTCCCAAATTTATCTCTAATAAATTGATAAAATTATTAATTTTGTGTCAATATATTTTTTAATTGTAAAAATTAAGAGATTTTTTAAATAGGAAATTTAAAGTTTAGAGATTAATCAGATTGTTTAGAAATTTATTAGATAATTCATTTAGATTTTTAATTAGTTACTCAACTTTATCTGTTTATTTAGTTTATAATTTAAAATTTTTTAATAAATCACTATCTTTTGACGTTATCAACTGTTTGAATACTTACCCGACATTTGTGTTGATATATCATATGGTCCTCGTAATAATATTTGGTTTAATAAGGAATTATTGTGAAATTACAGGGAATGAGCCAGGTTGCTCCTCCGCGACGAAGTCGTGTATTTACGGAATACAATACGGCGATCAATCTTTCTCCGTCGGATATTTTGCCAAAGAAACGCTAAGTCTATCGTCATATGACGTCATCGAAAACTTCCTGTTTGGATGCGGCCAAAACAACCGCGGACTCTTCGGTAGCGCCGCCGGCCTTCTCGGCCTCGGCCAGGACAAAATCTCCATCGTTAAACAGACCGCACAAAAGTACGGCCAGATCTTCTCCTACTGTCTCCCCAAAACTGCCAGTTCCACCGGCTACCTAACATTCGGCGGCGGCGGCAACGGCGGAGCGCTCAAATACACGCCGATCACGAAAGCGCACGGCGTCGCCAATTTCTACGGCCTCGACGTCGTCGGAATTAAGGTCGGCGGAACTCAGTTGCCGATTTCGTCGTCGGTCTTCTCGACCTCCGGCGCGATCATCGATTCCGGCACGGTGATCACGCGGCTGCCACCGGCGGCGTACGACGCCTTGAAATCGGCGTTTCAGAAAGGCATGACGGCGTATCCGAAGGCGCCGGAGCTGTCGATCCTGGACACGTGTTACGATCTGAGCAAGTACACGTCCGTAGACGTCCCGAAGGTGAGCTTCCTCTTCAAAGGTGGAACGGAACTCGATCTGGACGCCATCGGAATCTTGTACGGAGCATCGCCGGAGCAGGTTTGTTTGGCGTTCGCCGGAAATCAGGATCCGAGATCCGTCGCGATTATAGGGAACGTGCAGCAGAAGAGTTTGCAAGTGGTCTACGATGTCGGTGGAGGGAGGGTGGGGTTCGGCTATAATGGTTGTTAGGGGTAAAACTATAAATAGGCTTTCTTTGTGGGGCTAATGGGGGAAATAGGGATGATAATAAGGGCCAAAGGGCAATTGAAATTATTATACTTAAGAAATGTGTGGTAAAAAGGAAGATTTGAAAGAAGTTTGCAAATTGGGTATACTTTGCAAATGTATAGGGTTAAAACCTAAAAGCCTTTGTTTGTTACTTCTTATTGTTGTAATTGTAATTTGCAACAATTACCCATTAAAATGTTTGATGAAATTACAAAAATTATAACCAGAAATGACTGGAAATGTAAATTTGAAGAAATAATGAACTTATAGAACCGTTCACTCGTAGAAAAT

mRNA sequence

GTAAAACCATTGGAAAATCCACAACTGTACTGTGGAGTGGAGCATCGTCCTTTTATTTAAAAAGGGCTTAAGGGTACTTCTATCAACAACCCAAAAAAGAAAAAAGGAAAAAAAAAGCTCAATTTTCTCAGAGCTCTTCTGCAGCTCTAATCCATGGCGGCTCTCTCTCTCTCCTCTTTCTCACTCCTCTTCTTTTTCCTTTTCTTCCACTCGTTCGCCGGAAAGGTCGCGCCGGACGGCCACCACCTCACTGTCGAACTCGCCGCCCTTCTGCCCTCCGCCGCCTGCAGCCGCCGCTCTGCCAAAGTTACAGGTGTGAAAAAAGAAGATTCGTCTCCGTCTCTGGAGGTGGTTCACCGGCATGGGCCGTGCGCCGGAGCTTCAAAAGCGCCGAGCGAAGCGGAAATCTTTGTCCGCGATCAGGCTCGGGTGGACCTCATCCACTCCAAGTTCTCAGAGGAGTCTGGTTCGGGTTCGGTCCAGCGTATACGGCCGTCGAAGGCGACGAAGATTCCGGCGAAGTCCGGCGCCACCATCGGGTCCGGTAATTACATAGTGAGCGTCGGATTGGGGACGCCGAAGAAGGAGCTGTCGCTAATATTCGACACGGGGAGCGATCTGACGTGGACTCAGTGCGAGCCTTGCGCCAGATATTGCTACAGCCAGAAGGATCCGGTTTTCGCTCCTTCCAAATCCACCTCTTATTCCAATATCTCTTGTTCCTCCCCTTCCTGCTCTCAGCTTGAATCTGGCACCGGGAATGAGCCAGGTTGCTCCTCCGCGACGAAGTCGTGTATTTACGGAATACAATACGGCGATCAATCTTTCTCCGTCGGATATTTTGCCAAAGAAACGCTAAGTCTATCGTCATATGACGTCATCGAAAACTTCCTGTTTGGATGCGGCCAAAACAACCGCGGACTCTTCGGTAGCGCCGCCGGCCTTCTCGGCCTCGGCCAGGACAAAATCTCCATCGTTAAACAGACCGCACAAAAGTACGGCCAGATCTTCTCCTACTGTCTCCCCAAAACTGCCAGTTCCACCGGCTACCTAACATTCGGCGGCGGCGGCAACGGCGGAGCGCTCAAATACACGCCGATCACGAAAGCGCACGGCGTCGCCAATTTCTACGGCCTCGACGTCGTCGGAATTAAGGTCGGCGGAACTCAGTTGCCGATTTCGTCGTCGGTCTTCTCGACCTCCGGCGCGATCATCGATTCCGGCACGGTGATCACGCGGCTGCCACCGGCGGCGTACGACGCCTTGAAATCGGCGTTTCAGAAAGGCATGACGGCGTATCCGAAGGCGCCGGAGCTGTCGATCCTGGACACGTGTTACGATCTGAGCAAGTACACGTCCGTAGACGTCCCGAAGGTGAGCTTCCTCTTCAAAGGTGGAACGGAACTCGATCTGGACGCCATCGGAATCTTGTACGGAGCATCGCCGGAGCAGGTTTGTTTGGCGTTCGCCGGAAATCAGGATCCGAGATCCGTCGCGATTATAGGGAACGTGCAGCAGAAGAGTTTGCAAGTGGTCTACGATGTCGGTGGAGGGAGGGTGGGGTTCGGCTATAATGGTTGTTAGGGGTAAAACTATAAATAGGCTTTCTTTGTGGGGCTAATGGGGGAAATAGGGATGATAATAAGGGCCAAAGGGCAATTGAAATTATTATACTTAAGAAATGTGTGGTAAAAAGGAAGATTTGAAAGAAGTTTGCAAATTGGGTATACTTTGCAAATGTATAGGGTTAAAACCTAAAAGCCTTTGTTTGTTACTTCTTATTGTTGTAATTGTAATTTGCAACAATTACCCATTAAAATGTTTGATGAAATTACAAAAATTATAACCAGAAATGACTGGAAATGTAAATTTGAAGAAATAATGAACTTATAGAACCGTTCACTCGTAGAAAAT

Coding sequence (CDS)

ATGGCGGCTCTCTCTCTCTCCTCTTTCTCACTCCTCTTCTTTTTCCTTTTCTTCCACTCGTTCGCCGGAAAGGTCGCGCCGGACGGCCACCACCTCACTGTCGAACTCGCCGCCCTTCTGCCCTCCGCCGCCTGCAGCCGCCGCTCTGCCAAAGTTACAGGTGTGAAAAAAGAAGATTCGTCTCCGTCTCTGGAGGTGGTTCACCGGCATGGGCCGTGCGCCGGAGCTTCAAAAGCGCCGAGCGAAGCGGAAATCTTTGTCCGCGATCAGGCTCGGGTGGACCTCATCCACTCCAAGTTCTCAGAGGAGTCTGGTTCGGGTTCGGTCCAGCGTATACGGCCGTCGAAGGCGACGAAGATTCCGGCGAAGTCCGGCGCCACCATCGGGTCCGGTAATTACATAGTGAGCGTCGGATTGGGGACGCCGAAGAAGGAGCTGTCGCTAATATTCGACACGGGGAGCGATCTGACGTGGACTCAGTGCGAGCCTTGCGCCAGATATTGCTACAGCCAGAAGGATCCGGTTTTCGCTCCTTCCAAATCCACCTCTTATTCCAATATCTCTTGTTCCTCCCCTTCCTGCTCTCAGCTTGAATCTGGCACCGGGAATGAGCCAGGTTGCTCCTCCGCGACGAAGTCGTGTATTTACGGAATACAATACGGCGATCAATCTTTCTCCGTCGGATATTTTGCCAAAGAAACGCTAAGTCTATCGTCATATGACGTCATCGAAAACTTCCTGTTTGGATGCGGCCAAAACAACCGCGGACTCTTCGGTAGCGCCGCCGGCCTTCTCGGCCTCGGCCAGGACAAAATCTCCATCGTTAAACAGACCGCACAAAAGTACGGCCAGATCTTCTCCTACTGTCTCCCCAAAACTGCCAGTTCCACCGGCTACCTAACATTCGGCGGCGGCGGCAACGGCGGAGCGCTCAAATACACGCCGATCACGAAAGCGCACGGCGTCGCCAATTTCTACGGCCTCGACGTCGTCGGAATTAAGGTCGGCGGAACTCAGTTGCCGATTTCGTCGTCGGTCTTCTCGACCTCCGGCGCGATCATCGATTCCGGCACGGTGATCACGCGGCTGCCACCGGCGGCGTACGACGCCTTGAAATCGGCGTTTCAGAAAGGCATGACGGCGTATCCGAAGGCGCCGGAGCTGTCGATCCTGGACACGTGTTACGATCTGAGCAAGTACACGTCCGTAGACGTCCCGAAGGTGAGCTTCCTCTTCAAAGGTGGAACGGAACTCGATCTGGACGCCATCGGAATCTTGTACGGAGCATCGCCGGAGCAGGTTTGTTTGGCGTTCGCCGGAAATCAGGATCCGAGATCCGTCGCGATTATAGGGAACGTGCAGCAGAAGAGTTTGCAAGTGGTCTACGATGTCGGTGGAGGGAGGGTGGGGTTCGGCTATAATGGTTGTTAG

Protein sequence

MAALSLSSFSLLFFFLFFHSFAGKVAPDGHHLTVELAALLPSAACSRRSAKVTGVKKEDSSPSLEVVHRHGPCAGASKAPSEAEIFVRDQARVDLIHSKFSEESGSGSVQRIRPSKATKIPAKSGATIGSGNYIVSVGLGTPKKELSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSKSTSYSNISCSSPSCSQLESGTGNEPGCSSATKSCIYGIQYGDQSFSVGYFAKETLSLSSYDVIENFLFGCGQNNRGLFGSAAGLLGLGQDKISIVKQTAQKYGQIFSYCLPKTASSTGYLTFGGGGNGGALKYTPITKAHGVANFYGLDVVGIKVGGTQLPISSSVFSTSGAIIDSGTVITRLPPAAYDALKSAFQKGMTAYPKAPELSILDTCYDLSKYTSVDVPKVSFLFKGGTELDLDAIGILYGASPEQVCLAFAGNQDPRSVAIIGNVQQKSLQVVYDVGGGRVGFGYNGC

Homology

[Close]

BLAST of MC11g0385 vs. ExPASy Swiss-Prot
Match: Q8S9J6 (Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At5g10770 PE=2 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 2.6e-139
Identity = 255/453 (56.29%), Postives = 325/453 (71.74%), Query Frame = 0


Query: 28  DGHHLTVELAALLPSAACSRRSAKVTGVKKEDSSPSLEVVHRHGPCA----GASKAPSEA 87
           D H  T+++++LLPS++    S+ V   +   +  SL V HRHG C+    G + +P   
Sbjct: 32  DSH--TIQVSSLLPSSS----SSCVLSPRASTTKSSLHVTHRHGTCSRLNNGKATSPDHV 91

Query: 88  EIFVRDQARVDLIHSKFSEESGSGSVQRIRPSKATKIPAKSGATIGSGNYIVSVGLGTPK 147
           EI   DQARV+ IHSK S++  +  V     SK+T +PAK G+T+GSGNYIV+VGLGTPK
Sbjct: 92  EILRLDQARVNSIHSKLSKKLATDHVSE---SKSTDLPAKDGSTLGSGNYIVTVGLGTPK 151

Query: 148 KELSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSKSTSYSNISCSSPSCSQLESGTGN 207
            +LSLIFDTGSDLTWTQC+PC R CY QK+P+F PSKSTSY N+SCSS +C  L S TGN
Sbjct: 152 NDLSLIFDTGSDLTWTQCQPCVRTCYDQKEPIFNPSKSTSYYNVSCSSAACGSLSSATGN 211

Query: 208 EPGCSSATKSCIYGIQYGDQSFSVGYFAKETLSLSSYDVIENFLFGCGQNNRGLFGSAAG 267
              CS++  +CIYGIQYGDQSFSVG+ AKE  +L++ DV +   FGCG+NN+GLF   AG
Sbjct: 212 AGSCSAS--NCIYGIQYGDQSFSVGFLAKEKFTLTNSDVFDGVYFGCGENNQGLFTGVAG 271

Query: 268 LLGLGQDKISIVKQTAQKYGQIFSYCLPKTASSTGYLTFGGGGNGGALKYTPITKAHGVA 327
           LLGLG+DK+S   QTA  Y +IFSYCLP +AS TG+LTFG  G   ++K+TPI+      
Sbjct: 272 LLGLGRDKLSFPSQTATAYNKIFSYCLPSSASYTGHLTFGSAGISRSVKFTPISTITDGT 331

Query: 328 NFYGLDVVGIKVGGTQLPISSSVFSTSGAIIDSGTVITRLPPAAYDALKSAFQKGMTAYP 387
           +FYGL++V I VGG +LPI S+VFST GA+IDSGTVITRLPP AY AL+S+F+  M+ YP
Sbjct: 332 SFYGLNIVAITVGGQKLPIPSTVFSTPGALIDSGTVITRLPPKAYAALRSSFKAKMSKYP 391

Query: 388 KAPELSILDTCYDLSKYTSVDVPKVSFLFKGGTELDLDAIGILYGASPEQVCLAFAGNQD 447
               +SILDTC+DLS + +V +PKV+F F GG  ++L + GI Y     QVCLAFAGN D
Sbjct: 392 TTSGVSILDTCFDLSGFKTVTIPKVAFSFSGGAVVELGSKGIFYVFKISQVCLAFAGNSD 451

Query: 448 PRSVAIIGNVQQKSLQVVYDVGGGRVGFGYNGC 477
             + AI GNVQQ++L+VVYD  GGRVGF  NGC
Sbjct: 452 DSNAAIFGNVQQQTLEVVYDGAGGRVGFAPNGC 473

[Close]

BLAST of MC11g0385 vs. ExPASy Swiss-Prot
Match: Q9LEW3 (Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 2.7e-117
Identity = 225/447 (50.34%), Postives = 296/447 (66.22%), Query Frame = 0


Query: 33  TVELAALLPSAACSRRSAKVTGVKKEDSSPSLEVVHRHGPCAGASK--APSEAEIFVRDQ 92
           T+++++L PS++    S+K +  K      SL VVH HG C+  S        EI  RDQ
Sbjct: 39  TIQVSSLFPSSSSCVPSSKASNTKS-----SLRVVHMHGACSHLSSDARVDHDEIIRRDQ 98

Query: 93  ARVDLIHSKFSEESGSGSVQRIRPSKATKIPAKSGATIGSGNYIVSVGLGTPKKELSLIF 152
           ARV+ I+SK S+ S +     +  +K+T++PAKSG T+GSGNYIV++G+GTPK +LSL+F
Sbjct: 99  ARVESIYSKLSKNSAN----EVSEAKSTELPAKSGITLGSGNYIVTIGIGTPKHDLSLVF 158

Query: 153 DTGSDLTWTQCEPCARYCYSQKDPVFAPSKSTSYSNISCSSPSCSQLESGTGNEPGCSSA 212
           DTGSDLTWTQCEPC   CYSQK+P F PS S++Y N+SCSSP C   ES       CS++
Sbjct: 159 DTGSDLTWTQCEPCLGSCYSQKEPKFNPSSSSTYQNVSCSSPMCEDAES-------CSAS 218

Query: 213 TKSCIYGIQYGDQSFSVGYFAKETLSLSSYDVIENFLFGCGQNNRGLFGSAAGLLGLGQD 272
             +C+Y I YGD+SF+ G+ AKE  +L++ DV+E+  FGCG+NN+GLF   AGLLGLG  
Sbjct: 219 --NCVYSIVYGDKSFTQGFLAKEKFTLTNSDVLEDVYFGCGENNQGLFDGVAGLLGLGPG 278

Query: 273 KISIVKQTAQKYGQIFSYCLPK-TASSTGYLTFGGGGNGGALKYTPITKAHGVANFYGLD 332
           K+S+  QT   Y  IFSYCLP  T++STG+LTFG  G   ++K+TPI+      N YG+D
Sbjct: 279 KLSLPAQTTTTYNNIFSYCLPSFTSNSTGHLTFGSAGISESVKFTPISSFPSAFN-YGID 338

Query: 333 VVGIKVGGTQLPISSSVFSTSGAIIDSGTVITRLPPAAYDALKSAFQKGMTAYPKAPELS 392
           ++GI VG  +L I+ + FST GAIIDSGTV TRLP   Y  L+S F++ M++Y       
Sbjct: 339 IIGISVGDKELAITPNSFSTEGAIIDSGTVFTRLPTKVYAELRSVFKEKMSSYKSTSGYG 398

Query: 393 ILDTCYDLSKYTSVDVPKVSFLFKGGTELDLDAIGILYGASPEQVCLAFAGNQDPRSVAI 452
           + DTCYD +   +V  P ++F F G T ++LD  GI       QVCLAFAGN D    AI
Sbjct: 399 LFDTCYDFTGLDTVTYPTIAFSFAGSTVVELDGSGISLPIKISQVCLAFAGNDD--LPAI 458

Query: 453 IGNVQQKSLQVVYDVGGGRVGFGYNGC 477
            GNVQQ +L VVYDV GGRVGF  NGC
Sbjct: 459 FGNVQQTTLDVVYDVAGGRVGFAPNGC 464

[Close]

BLAST of MC11g0385 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 2.6e-75
Identity = 181/486 (37.24%), Postives = 263/486 (54.12%), Query Frame = 0


Query: 9   FSLLFFFLFFHSFAG-----KVAPDGHHL--TVELAALLPSAACSRRSAKVTGVKKEDSS 68
           FSL FFFL   SF+       + P+ H L     ++    S + S   ++       +SS
Sbjct: 10  FSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSFQPDSDSESLLESEFESGSDSESS 69

Query: 69  PSLEVVHRHGPCAGASKAPSE--AEIFVRDQARVDLIHSKFSEESGSGSVQRIRPSKATK 128
            S+ +   H     ++K P E  +    RD  RV  I +  ++  G       RP   + 
Sbjct: 70  SSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPRPGGFSS 129

Query: 129 IPAKSGATIGSGNYIVSVGLGTPKKELSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPS 188
               SG + GSG Y   +G+GTP + + ++ DTGSD+ W QC PC R CYSQ DP+F P 
Sbjct: 130 -SVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRR-CYSQSDPIFDPR 189

Query: 189 KSTSYSNISCSSPSCSQLESGTGNEPGCSSATKSCIYGIQYGDQSFSVGYFAKETLSLSS 248
           KS +Y+ I CSSP C +L+S      GC++  K+C+Y + YGD SF+VG F+ ETL+   
Sbjct: 190 KSKTYATIPCSSPHCRRLDSA-----GCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRR 249

Query: 249 YDVIENFLFGCGQNNRGLFGSAAGLLGLGQDKISIVKQTAQKYGQIFSYCLPKTASST-- 308
            + ++    GCG +N GLF  AAGLLGLG+ K+S   QT  ++ Q FSYCL   ++S+  
Sbjct: 250 -NRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKP 309

Query: 309 GYLTFGGGGNGGALKYTPITKAHGVANFYGLDVVGIKVGGTQLP-ISSSVF-----STSG 368
             + FG        ++TP+     +  FY + ++GI VGGT++P +++S+F        G
Sbjct: 310 SSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGG 369

Query: 369 AIIDSGTVITRLPPAAYDALKSAFQKGMTAYPKAPELSILDTCYDLSKYTSVDVPKVSFL 428
            IIDSGT +TRL   AY A++ AF+ G     +AP+ S+ DTC+DLS    V VP V   
Sbjct: 370 VIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLH 429

Query: 429 FKGGTELDLDAIGILYGASPE-QVCLAFAGNQDPRSVAIIGNVQQKSLQVVYDVGGGRVG 477
           F+ G ++ L A   L       + C AFAG      ++IIGN+QQ+  +VVYD+   RVG
Sbjct: 430 FR-GADVSLPATNYLIPVDTNGKFCFAFAGTMG--GLSIIGNIQQQGFRVVYDLASSRVG 484

[Close]

BLAST of MC11g0385 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 4.8e-69
Identity = 148/360 (41.11%), Postives = 204/360 (56.67%), Query Frame = 0


Query: 124 SGATIGSGNYIVSVGLGTPKKELSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSKSTS 183
           SG   GSG Y V +G+G+P ++  ++ D+GSD+ W QC+PC + CY Q DPVF P+KS S
Sbjct: 122 SGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPC-KLCYKQSDPVFDPAKSGS 181

Query: 184 YSNISCSSPSCSQLESGTGNEPGCSSATKSCIYGIQYGDQSFSVGYFAKETLSLSSYDVI 243
           Y+ +SC S  C ++E+      GC S    C Y + YGD S++ G  A ETL+ +   V+
Sbjct: 182 YTGVSCGSSVCDRIENS-----GCHSG--GCRYEVMYGDGSYTKGTLALETLTFAK-TVV 241

Query: 244 ENFLFGCGQNNRGLFGSAAGLLGLGQDKISIVKQTAQKYGQIFSYCL-PKTASSTGYLTF 303
            N   GCG  NRG+F  AAGLLG+G   +S V Q + + G  F YCL  +   STG L F
Sbjct: 242 RNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDSTGSLVF 301

Query: 304 GGGGNGGALKYTPITKAHGVANFYGLDVVGIKVGGTQLPISSSVFSTS-----GAIIDSG 363
           G         + P+ +     +FY + + G+ VGG ++P+   VF  +     G ++D+G
Sbjct: 302 GREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTG 361

Query: 364 TVITRLPPAAYDALKSAFQKGMTAYPKAPELSILDTCYDLSKYTSVDVPKVSFLFKGGTE 423
           T +TRLP AAY A +  F+      P+A  +SI DTCYDLS + SV VP VSF F  G  
Sbjct: 362 TAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPV 421

Query: 424 LDLDAIGILYGASPE-QVCLAFAGNQDPRSVAIIGNVQQKSLQVVYDVGGGRVGFGYNGC 477
           L L A   L         C AFA +  P  ++IIGN+QQ+ +QV +D   G VGFG N C
Sbjct: 422 LTLPARNFLMPVDDSGTYCFAFAAS--PTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

[Close]

BLAST of MC11g0385 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 2.4e-65
Identity = 147/364 (40.38%), Postives = 202/364 (55.49%), Query Frame = 0


Query: 121 PAKSGATIGSGNYIVSVGLGTPKKELSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSK 180
           P  SGA+ GSG Y   +G+GTP KE+ L+ DTGSD+ W QCEPCA  CY Q DPVF P+ 
Sbjct: 150 PVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCAD-CYQQSDPVFNPTS 209

Query: 181 STSYSNISCSSPSCSQLESGTGNEPGCSSATKSCIYGIQYGDQSFSVGYFAKETLSLSSY 240
           S++Y +++CS+P CS LE+       C S    C+Y + YGD SF+VG  A +T++  + 
Sbjct: 210 SSTYKSLTCSAPQCSLLETS-----ACRS--NKCLYQVSYGDGSFTVGELATDTVTFGNS 269

Query: 241 DVIENFLFGCGQNNRGLFGSAAGLLGLGQDKISIVKQTAQKYGQIFSYCL-PKTASSTGY 300
             I N   GCG +N GLF  AAGLLGLG   +SI   T Q     FSYCL  + +  +  
Sbjct: 270 GKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSI---TNQMKATSFSYCLVDRDSGKSSS 329

Query: 301 LTFGGGGNGGALKYTPITKAHGVANFYGLDVVGIKVGGTQLPISSSVF-----STSGAII 360
           L F     GG     P+ +   +  FY + + G  VGG ++ +  ++F      + G I+
Sbjct: 330 LDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVIL 389

Query: 361 DSGTVITRLPPAAYDALKSAFQKGMTAYPK-APELSILDTCYDLSKYTSVDVPKVSFLFK 420
           D GT +TRL   AY++L+ AF K      K +  +S+ DTCYD S  ++V VP V+F F 
Sbjct: 390 DCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFT 449

Query: 421 GGTELDLDAIGILYGASPE-QVCLAFAGNQDPRSVAIIGNVQQKSLQVVYDVGGGRVGFG 477
           GG  LDL A   L         C AFA      S++IIGNVQQ+  ++ YD+    +G  
Sbjct: 450 GGKSLDLPAKNYLIPVDDSGTFCFAFAPTSS--SLSIIGNVQQQGTRITYDLSKNVIGLS 500

[Close]

BLAST of MC11g0385 vs. NCBI nr
Match: XP_022138035.1 (aspartyl protease family protein At5g10770-like isoform X1 [Momordica charantia])

HSP 1 Score: 941 bits (2433), Expect = 0.0
Identity = 476/476 (100.00%), Postives = 476/476 (100.00%), Query Frame = 0


Query: 1   MAALSLSSFSLLFFFLFFHSFAGKVAPDGHHLTVELAALLPSAACSRRSAKVTGVKKEDS 60
           MAALSLSSFSLLFFFLFFHSFAGKVAPDGHHLTVELAALLPSAACSRRSAKVTGVKKEDS
Sbjct: 1   MAALSLSSFSLLFFFLFFHSFAGKVAPDGHHLTVELAALLPSAACSRRSAKVTGVKKEDS 60

Query: 61  SPSLEVVHRHGPCAGASKAPSEAEIFVRDQARVDLIHSKFSEESGSGSVQRIRPSKATKI 120
           SPSLEVVHRHGPCAGASKAPSEAEIFVRDQARVDLIHSKFSEESGSGSVQRIRPSKATKI
Sbjct: 61  SPSLEVVHRHGPCAGASKAPSEAEIFVRDQARVDLIHSKFSEESGSGSVQRIRPSKATKI 120

Query: 121 PAKSGATIGSGNYIVSVGLGTPKKELSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSK 180
           PAKSGATIGSGNYIVSVGLGTPKKELSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSK
Sbjct: 121 PAKSGATIGSGNYIVSVGLGTPKKELSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSK 180

Query: 181 STSYSNISCSSPSCSQLESGTGNEPGCSSATKSCIYGIQYGDQSFSVGYFAKETLSLSSY 240
           STSYSNISCSSPSCSQLESGTGNEPGCSSATKSCIYGIQYGDQSFSVGYFAKETLSLSSY
Sbjct: 181 STSYSNISCSSPSCSQLESGTGNEPGCSSATKSCIYGIQYGDQSFSVGYFAKETLSLSSY 240

Query: 241 DVIENFLFGCGQNNRGLFGSAAGLLGLGQDKISIVKQTAQKYGQIFSYCLPKTASSTGYL 300
           DVIENFLFGCGQNNRGLFGSAAGLLGLGQDKISIVKQTAQKYGQIFSYCLPKTASSTGYL
Sbjct: 241 DVIENFLFGCGQNNRGLFGSAAGLLGLGQDKISIVKQTAQKYGQIFSYCLPKTASSTGYL 300

Query: 301 TFGGGGNGGALKYTPITKAHGVANFYGLDVVGIKVGGTQLPISSSVFSTSGAIIDSGTVI 360
           TFGGGGNGGALKYTPITKAHGVANFYGLDVVGIKVGGTQLPISSSVFSTSGAIIDSGTVI
Sbjct: 301 TFGGGGNGGALKYTPITKAHGVANFYGLDVVGIKVGGTQLPISSSVFSTSGAIIDSGTVI 360

Query: 361 TRLPPAAYDALKSAFQKGMTAYPKAPELSILDTCYDLSKYTSVDVPKVSFLFKGGTELDL 420
           TRLPPAAYDALKSAFQKGMTAYPKAPELSILDTCYDLSKYTSVDVPKVSFLFKGGTELDL
Sbjct: 361 TRLPPAAYDALKSAFQKGMTAYPKAPELSILDTCYDLSKYTSVDVPKVSFLFKGGTELDL 420

Query: 421 DAIGILYGASPEQVCLAFAGNQDPRSVAIIGNVQQKSLQVVYDVGGGRVGFGYNGC 476
           DAIGILYGASPEQVCLAFAGNQDPRSVAIIGNVQQKSLQVVYDVGGGRVGFGYNGC
Sbjct: 421 DAIGILYGASPEQVCLAFAGNQDPRSVAIIGNVQQKSLQVVYDVGGGRVGFGYNGC 476

[Close]

BLAST of MC11g0385 vs. NCBI nr
Match: XP_022138043.1 (aspartyl protease family protein At5g10770-like isoform X2 [Momordica charantia])

HSP 1 Score: 933 bits (2411), Expect = 0.0
Identity = 474/476 (99.58%), Postives = 474/476 (99.58%), Query Frame = 0


Query: 1   MAALSLSSFSLLFFFLFFHSFAGKVAPDGHHLTVELAALLPSAACSRRSAKVTGVKKEDS 60
           MAALSLSSFSLLFFFLFFHSFAGKVAPDGHHLTVELAALLPSAACSRRSAK  GVKKEDS
Sbjct: 1   MAALSLSSFSLLFFFLFFHSFAGKVAPDGHHLTVELAALLPSAACSRRSAK--GVKKEDS 60

Query: 61  SPSLEVVHRHGPCAGASKAPSEAEIFVRDQARVDLIHSKFSEESGSGSVQRIRPSKATKI 120
           SPSLEVVHRHGPCAGASKAPSEAEIFVRDQARVDLIHSKFSEESGSGSVQRIRPSKATKI
Sbjct: 61  SPSLEVVHRHGPCAGASKAPSEAEIFVRDQARVDLIHSKFSEESGSGSVQRIRPSKATKI 120

Query: 121 PAKSGATIGSGNYIVSVGLGTPKKELSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSK 180
           PAKSGATIGSGNYIVSVGLGTPKKELSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSK
Sbjct: 121 PAKSGATIGSGNYIVSVGLGTPKKELSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSK 180

Query: 181 STSYSNISCSSPSCSQLESGTGNEPGCSSATKSCIYGIQYGDQSFSVGYFAKETLSLSSY 240
           STSYSNISCSSPSCSQLESGTGNEPGCSSATKSCIYGIQYGDQSFSVGYFAKETLSLSSY
Sbjct: 181 STSYSNISCSSPSCSQLESGTGNEPGCSSATKSCIYGIQYGDQSFSVGYFAKETLSLSSY 240

Query: 241 DVIENFLFGCGQNNRGLFGSAAGLLGLGQDKISIVKQTAQKYGQIFSYCLPKTASSTGYL 300
           DVIENFLFGCGQNNRGLFGSAAGLLGLGQDKISIVKQTAQKYGQIFSYCLPKTASSTGYL
Sbjct: 241 DVIENFLFGCGQNNRGLFGSAAGLLGLGQDKISIVKQTAQKYGQIFSYCLPKTASSTGYL 300

Query: 301 TFGGGGNGGALKYTPITKAHGVANFYGLDVVGIKVGGTQLPISSSVFSTSGAIIDSGTVI 360
           TFGGGGNGGALKYTPITKAHGVANFYGLDVVGIKVGGTQLPISSSVFSTSGAIIDSGTVI
Sbjct: 301 TFGGGGNGGALKYTPITKAHGVANFYGLDVVGIKVGGTQLPISSSVFSTSGAIIDSGTVI 360

Query: 361 TRLPPAAYDALKSAFQKGMTAYPKAPELSILDTCYDLSKYTSVDVPKVSFLFKGGTELDL 420
           TRLPPAAYDALKSAFQKGMTAYPKAPELSILDTCYDLSKYTSVDVPKVSFLFKGGTELDL
Sbjct: 361 TRLPPAAYDALKSAFQKGMTAYPKAPELSILDTCYDLSKYTSVDVPKVSFLFKGGTELDL 420

Query: 421 DAIGILYGASPEQVCLAFAGNQDPRSVAIIGNVQQKSLQVVYDVGGGRVGFGYNGC 476
           DAIGILYGASPEQVCLAFAGNQDPRSVAIIGNVQQKSLQVVYDVGGGRVGFGYNGC
Sbjct: 421 DAIGILYGASPEQVCLAFAGNQDPRSVAIIGNVQQKSLQVVYDVGGGRVGFGYNGC 474

[Close]

BLAST of MC11g0385 vs. NCBI nr
Match: XP_022936652.1 (aspartyl protease family protein At5g10770-like isoform X1 [Cucurbita moschata] >KAG6591512.1 Aspartyl protease family protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 762 bits (1968), Expect = 1.12e-274
Identity = 388/472 (82.20%), Postives = 422/472 (89.41%), Query Frame = 0


Query: 10  SLLFF---FLFFHSFAGKVAPDGHHLTVELAALLPSAACSRRSAKVTGVKKEDSSPSLEV 69
           S+LFF   FLFF+S AGKV+PD H LTVELA LLPSAAC+RRS +     +   S SLEV
Sbjct: 6   SMLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQDSGRRTQS-SLEV 65

Query: 70  VHRHGPCAGA-SKAPSEAEIFVRDQARVDLIHSKFSEESGSGSVQRIRPSKATKIPAKSG 129
           +HRHGPC G  SKAP+ AE+FV+DQARVD IHSKFS +  S  + R+RPSKATKIPAKSG
Sbjct: 66  IHRHGPCGGGESKAPTAAELFVQDQARVDFIHSKFSGDFKS--IDRLRPSKATKIPAKSG 125

Query: 130 ATIGSGNYIVSVGLGTPKKELSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSKSTSYS 189
           ATIGSGNYIV+V LGTPKK LSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPS+ST+YS
Sbjct: 126 ATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTTYS 185

Query: 190 NISCSSPSCSQLESGTGNEPGCSSATKSCIYGIQYGDQSFSVGYFAKETLSLSSYDVIEN 249
           NI+CSSP CSQLESGTGN+PGCS+A KSCIYGIQYGDQSFSVGYFAKETL+L+  DVI N
Sbjct: 186 NITCSSPICSQLESGTGNQPGCSAA-KSCIYGIQYGDQSFSVGYFAKETLTLTPTDVISN 245

Query: 250 FLFGCGQNNRGLFGSAAGLLGLGQDKISIVKQTAQKYGQIFSYCLPKTASSTGYLTFGG- 309
           FLFGCGQNNRGLFGSAAGL+GLGQDKISIVKQTAQKYGQIFSYCLPKT+SSTGYL FGG 
Sbjct: 246 FLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGGS 305

Query: 310 GGNGGALKYTPITKAHGVANFYGLDVVGIKVGGTQLPISSSVFSTSGAIIDSGTVITRLP 369
           GG GGALKYTPITKAHGVANFYG+D+VG+KVGG Q+PIS+SVFSTSGAIIDSGTVITRLP
Sbjct: 306 GGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRLP 365

Query: 370 PAAYDALKSAFQKGMTAYPKAPELSILDTCYDLSKYTSVDVPKVSFLFKGGTELDLDAIG 429
           P AY ALKSAFQKGMT YPKAPELSILDTCYDLSKYTSV++PKV FLFKGGT L+LD  G
Sbjct: 366 PDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGTG 425

Query: 430 ILYGASPEQVCLAFAGNQDPRSVAIIGNVQQKSLQVVYDVGGGRVGFGYNGC 476
           ILYGAS  QVCLAFAGN DP SVAIIGNVQQK+LQVVYDVGGG++GFGYNGC
Sbjct: 426 ILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 473

[Close]

BLAST of MC11g0385 vs. NCBI nr
Match: XP_022936653.1 (aspartyl protease family protein At5g10770-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 761 bits (1965), Expect = 2.76e-274
Identity = 388/472 (82.20%), Postives = 422/472 (89.41%), Query Frame = 0


Query: 10  SLLFF---FLFFHSFAGKVAPDGHHLTVELAALLPSAACSRRSAKVTGVKKEDSSPSLEV 69
           S+LFF   FLFF+S AGKV+PD H LTVELA LLPSAAC+RRS     V+   +  SLEV
Sbjct: 6   SMLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRS-----VQGRRTQSSLEV 65

Query: 70  VHRHGPCAGA-SKAPSEAEIFVRDQARVDLIHSKFSEESGSGSVQRIRPSKATKIPAKSG 129
           +HRHGPC G  SKAP+ AE+FV+DQARVD IHSKFS +  S  + R+RPSKATKIPAKSG
Sbjct: 66  IHRHGPCGGGESKAPTAAELFVQDQARVDFIHSKFSGDFKS--IDRLRPSKATKIPAKSG 125

Query: 130 ATIGSGNYIVSVGLGTPKKELSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSKSTSYS 189
           ATIGSGNYIV+V LGTPKK LSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPS+ST+YS
Sbjct: 126 ATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTTYS 185

Query: 190 NISCSSPSCSQLESGTGNEPGCSSATKSCIYGIQYGDQSFSVGYFAKETLSLSSYDVIEN 249
           NI+CSSP CSQLESGTGN+PGCS+A KSCIYGIQYGDQSFSVGYFAKETL+L+  DVI N
Sbjct: 186 NITCSSPICSQLESGTGNQPGCSAA-KSCIYGIQYGDQSFSVGYFAKETLTLTPTDVISN 245

Query: 250 FLFGCGQNNRGLFGSAAGLLGLGQDKISIVKQTAQKYGQIFSYCLPKTASSTGYLTFGG- 309
           FLFGCGQNNRGLFGSAAGL+GLGQDKISIVKQTAQKYGQIFSYCLPKT+SSTGYL FGG 
Sbjct: 246 FLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGGS 305

Query: 310 GGNGGALKYTPITKAHGVANFYGLDVVGIKVGGTQLPISSSVFSTSGAIIDSGTVITRLP 369
           GG GGALKYTPITKAHGVANFYG+D+VG+KVGG Q+PIS+SVFSTSGAIIDSGTVITRLP
Sbjct: 306 GGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRLP 365

Query: 370 PAAYDALKSAFQKGMTAYPKAPELSILDTCYDLSKYTSVDVPKVSFLFKGGTELDLDAIG 429
           P AY ALKSAFQKGMT YPKAPELSILDTCYDLSKYTSV++PKV FLFKGGT L+LD  G
Sbjct: 366 PDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGTG 425

Query: 430 ILYGASPEQVCLAFAGNQDPRSVAIIGNVQQKSLQVVYDVGGGRVGFGYNGC 476
           ILYGAS  QVCLAFAGN DP SVAIIGNVQQK+LQVVYDVGGG++GFGYNGC
Sbjct: 426 ILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 469

[Close]

BLAST of MC11g0385 vs. NCBI nr
Match: KAG7024399.1 (Aspartyl protease family protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 760 bits (1963), Expect = 6.45e-274
Identity = 387/472 (81.99%), Postives = 421/472 (89.19%), Query Frame = 0


Query: 10  SLLFF---FLFFHSFAGKVAPDGHHLTVELAALLPSAACSRRSAKVTGVKKEDSSPSLEV 69
           S+LFF   FLFF+S AGKV+PD H LTVELA LLPSAAC+RRS +     +   S SLEV
Sbjct: 6   SMLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQDSGRRTQS-SLEV 65

Query: 70  VHRHGPCAGA-SKAPSEAEIFVRDQARVDLIHSKFSEESGSGSVQRIRPSKATKIPAKSG 129
           +HRHGPC G  SKAP+  E+FV+DQARVD IHSKFS +  S  + R+RPSKATKIPAKSG
Sbjct: 66  IHRHGPCGGGESKAPTAEELFVQDQARVDFIHSKFSGDFKS--IDRLRPSKATKIPAKSG 125

Query: 130 ATIGSGNYIVSVGLGTPKKELSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSKSTSYS 189
           ATIGSGNYIV+V LGTPKK LSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPS+ST+YS
Sbjct: 126 ATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTTYS 185

Query: 190 NISCSSPSCSQLESGTGNEPGCSSATKSCIYGIQYGDQSFSVGYFAKETLSLSSYDVIEN 249
           NI+CSSP CSQLESGTGN+PGCS+A KSCIYGIQYGDQSFSVGYFAKETL+L+  DVI N
Sbjct: 186 NITCSSPICSQLESGTGNQPGCSAA-KSCIYGIQYGDQSFSVGYFAKETLTLTPTDVISN 245

Query: 250 FLFGCGQNNRGLFGSAAGLLGLGQDKISIVKQTAQKYGQIFSYCLPKTASSTGYLTFGG- 309
           FLFGCGQNNRGLFGSAAGL+GLGQDKISIVKQTAQKYGQIFSYCLPKT+SSTGYL FGG 
Sbjct: 246 FLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGGS 305

Query: 310 GGNGGALKYTPITKAHGVANFYGLDVVGIKVGGTQLPISSSVFSTSGAIIDSGTVITRLP 369
           GG GGALKYTPITKAHGVANFYG+D+VG+KVGG Q+PIS+SVFSTSGAIIDSGTVITRLP
Sbjct: 306 GGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRLP 365

Query: 370 PAAYDALKSAFQKGMTAYPKAPELSILDTCYDLSKYTSVDVPKVSFLFKGGTELDLDAIG 429
           P AY ALKSAFQKGMT YPKAPELSILDTCYDLSKYTSV++PKV FLFKGGT L+LD  G
Sbjct: 366 PDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGTG 425

Query: 430 ILYGASPEQVCLAFAGNQDPRSVAIIGNVQQKSLQVVYDVGGGRVGFGYNGC 476
           ILYGAS  QVCLAFAGN DP SVAIIGNVQQK+LQVVYDVGGG++GFGYNGC
Sbjct: 426 ILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 473

[Close]

BLAST of MC11g0385 vs. ExPASy TrEMBL
Match: A0A6J1C8A9 (aspartyl protease family protein At5g10770-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111009289 PE=3 SV=1)

HSP 1 Score: 941 bits (2433), Expect = 0.0
Identity = 476/476 (100.00%), Postives = 476/476 (100.00%), Query Frame = 0


Query: 1   MAALSLSSFSLLFFFLFFHSFAGKVAPDGHHLTVELAALLPSAACSRRSAKVTGVKKEDS 60
           MAALSLSSFSLLFFFLFFHSFAGKVAPDGHHLTVELAALLPSAACSRRSAKVTGVKKEDS
Sbjct: 1   MAALSLSSFSLLFFFLFFHSFAGKVAPDGHHLTVELAALLPSAACSRRSAKVTGVKKEDS 60

Query: 61  SPSLEVVHRHGPCAGASKAPSEAEIFVRDQARVDLIHSKFSEESGSGSVQRIRPSKATKI 120
           SPSLEVVHRHGPCAGASKAPSEAEIFVRDQARVDLIHSKFSEESGSGSVQRIRPSKATKI
Sbjct: 61  SPSLEVVHRHGPCAGASKAPSEAEIFVRDQARVDLIHSKFSEESGSGSVQRIRPSKATKI 120

Query: 121 PAKSGATIGSGNYIVSVGLGTPKKELSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSK 180
           PAKSGATIGSGNYIVSVGLGTPKKELSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSK
Sbjct: 121 PAKSGATIGSGNYIVSVGLGTPKKELSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSK 180

Query: 181 STSYSNISCSSPSCSQLESGTGNEPGCSSATKSCIYGIQYGDQSFSVGYFAKETLSLSSY 240
           STSYSNISCSSPSCSQLESGTGNEPGCSSATKSCIYGIQYGDQSFSVGYFAKETLSLSSY
Sbjct: 181 STSYSNISCSSPSCSQLESGTGNEPGCSSATKSCIYGIQYGDQSFSVGYFAKETLSLSSY 240

Query: 241 DVIENFLFGCGQNNRGLFGSAAGLLGLGQDKISIVKQTAQKYGQIFSYCLPKTASSTGYL 300
           DVIENFLFGCGQNNRGLFGSAAGLLGLGQDKISIVKQTAQKYGQIFSYCLPKTASSTGYL
Sbjct: 241 DVIENFLFGCGQNNRGLFGSAAGLLGLGQDKISIVKQTAQKYGQIFSYCLPKTASSTGYL 300

Query: 301 TFGGGGNGGALKYTPITKAHGVANFYGLDVVGIKVGGTQLPISSSVFSTSGAIIDSGTVI 360
           TFGGGGNGGALKYTPITKAHGVANFYGLDVVGIKVGGTQLPISSSVFSTSGAIIDSGTVI
Sbjct: 301 TFGGGGNGGALKYTPITKAHGVANFYGLDVVGIKVGGTQLPISSSVFSTSGAIIDSGTVI 360

Query: 361 TRLPPAAYDALKSAFQKGMTAYPKAPELSILDTCYDLSKYTSVDVPKVSFLFKGGTELDL 420
           TRLPPAAYDALKSAFQKGMTAYPKAPELSILDTCYDLSKYTSVDVPKVSFLFKGGTELDL
Sbjct: 361 TRLPPAAYDALKSAFQKGMTAYPKAPELSILDTCYDLSKYTSVDVPKVSFLFKGGTELDL 420

Query: 421 DAIGILYGASPEQVCLAFAGNQDPRSVAIIGNVQQKSLQVVYDVGGGRVGFGYNGC 476
           DAIGILYGASPEQVCLAFAGNQDPRSVAIIGNVQQKSLQVVYDVGGGRVGFGYNGC
Sbjct: 421 DAIGILYGASPEQVCLAFAGNQDPRSVAIIGNVQQKSLQVVYDVGGGRVGFGYNGC 476

[Close]

BLAST of MC11g0385 vs. ExPASy TrEMBL
Match: A0A6J1CBZ3 (aspartyl protease family protein At5g10770-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111009289 PE=3 SV=1)

HSP 1 Score: 933 bits (2411), Expect = 0.0
Identity = 474/476 (99.58%), Postives = 474/476 (99.58%), Query Frame = 0


Query: 1   MAALSLSSFSLLFFFLFFHSFAGKVAPDGHHLTVELAALLPSAACSRRSAKVTGVKKEDS 60
           MAALSLSSFSLLFFFLFFHSFAGKVAPDGHHLTVELAALLPSAACSRRSAK  GVKKEDS
Sbjct: 1   MAALSLSSFSLLFFFLFFHSFAGKVAPDGHHLTVELAALLPSAACSRRSAK--GVKKEDS 60

Query: 61  SPSLEVVHRHGPCAGASKAPSEAEIFVRDQARVDLIHSKFSEESGSGSVQRIRPSKATKI 120
           SPSLEVVHRHGPCAGASKAPSEAEIFVRDQARVDLIHSKFSEESGSGSVQRIRPSKATKI
Sbjct: 61  SPSLEVVHRHGPCAGASKAPSEAEIFVRDQARVDLIHSKFSEESGSGSVQRIRPSKATKI 120

Query: 121 PAKSGATIGSGNYIVSVGLGTPKKELSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSK 180
           PAKSGATIGSGNYIVSVGLGTPKKELSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSK
Sbjct: 121 PAKSGATIGSGNYIVSVGLGTPKKELSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSK 180

Query: 181 STSYSNISCSSPSCSQLESGTGNEPGCSSATKSCIYGIQYGDQSFSVGYFAKETLSLSSY 240
           STSYSNISCSSPSCSQLESGTGNEPGCSSATKSCIYGIQYGDQSFSVGYFAKETLSLSSY
Sbjct: 181 STSYSNISCSSPSCSQLESGTGNEPGCSSATKSCIYGIQYGDQSFSVGYFAKETLSLSSY 240

Query: 241 DVIENFLFGCGQNNRGLFGSAAGLLGLGQDKISIVKQTAQKYGQIFSYCLPKTASSTGYL 300
           DVIENFLFGCGQNNRGLFGSAAGLLGLGQDKISIVKQTAQKYGQIFSYCLPKTASSTGYL
Sbjct: 241 DVIENFLFGCGQNNRGLFGSAAGLLGLGQDKISIVKQTAQKYGQIFSYCLPKTASSTGYL 300

Query: 301 TFGGGGNGGALKYTPITKAHGVANFYGLDVVGIKVGGTQLPISSSVFSTSGAIIDSGTVI 360
           TFGGGGNGGALKYTPITKAHGVANFYGLDVVGIKVGGTQLPISSSVFSTSGAIIDSGTVI
Sbjct: 301 TFGGGGNGGALKYTPITKAHGVANFYGLDVVGIKVGGTQLPISSSVFSTSGAIIDSGTVI 360

Query: 361 TRLPPAAYDALKSAFQKGMTAYPKAPELSILDTCYDLSKYTSVDVPKVSFLFKGGTELDL 420
           TRLPPAAYDALKSAFQKGMTAYPKAPELSILDTCYDLSKYTSVDVPKVSFLFKGGTELDL
Sbjct: 361 TRLPPAAYDALKSAFQKGMTAYPKAPELSILDTCYDLSKYTSVDVPKVSFLFKGGTELDL 420

Query: 421 DAIGILYGASPEQVCLAFAGNQDPRSVAIIGNVQQKSLQVVYDVGGGRVGFGYNGC 476
           DAIGILYGASPEQVCLAFAGNQDPRSVAIIGNVQQKSLQVVYDVGGGRVGFGYNGC
Sbjct: 421 DAIGILYGASPEQVCLAFAGNQDPRSVAIIGNVQQKSLQVVYDVGGGRVGFGYNGC 474

[Close]

BLAST of MC11g0385 vs. ExPASy TrEMBL
Match: A0A6J1F908 (aspartyl protease family protein At5g10770-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443184 PE=3 SV=1)

HSP 1 Score: 762 bits (1968), Expect = 5.41e-275
Identity = 388/472 (82.20%), Postives = 422/472 (89.41%), Query Frame = 0


Query: 10  SLLFF---FLFFHSFAGKVAPDGHHLTVELAALLPSAACSRRSAKVTGVKKEDSSPSLEV 69
           S+LFF   FLFF+S AGKV+PD H LTVELA LLPSAAC+RRS +     +   S SLEV
Sbjct: 6   SMLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQDSGRRTQS-SLEV 65

Query: 70  VHRHGPCAGA-SKAPSEAEIFVRDQARVDLIHSKFSEESGSGSVQRIRPSKATKIPAKSG 129
           +HRHGPC G  SKAP+ AE+FV+DQARVD IHSKFS +  S  + R+RPSKATKIPAKSG
Sbjct: 66  IHRHGPCGGGESKAPTAAELFVQDQARVDFIHSKFSGDFKS--IDRLRPSKATKIPAKSG 125

Query: 130 ATIGSGNYIVSVGLGTPKKELSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSKSTSYS 189
           ATIGSGNYIV+V LGTPKK LSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPS+ST+YS
Sbjct: 126 ATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTTYS 185

Query: 190 NISCSSPSCSQLESGTGNEPGCSSATKSCIYGIQYGDQSFSVGYFAKETLSLSSYDVIEN 249
           NI+CSSP CSQLESGTGN+PGCS+A KSCIYGIQYGDQSFSVGYFAKETL+L+  DVI N
Sbjct: 186 NITCSSPICSQLESGTGNQPGCSAA-KSCIYGIQYGDQSFSVGYFAKETLTLTPTDVISN 245

Query: 250 FLFGCGQNNRGLFGSAAGLLGLGQDKISIVKQTAQKYGQIFSYCLPKTASSTGYLTFGG- 309
           FLFGCGQNNRGLFGSAAGL+GLGQDKISIVKQTAQKYGQIFSYCLPKT+SSTGYL FGG 
Sbjct: 246 FLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGGS 305

Query: 310 GGNGGALKYTPITKAHGVANFYGLDVVGIKVGGTQLPISSSVFSTSGAIIDSGTVITRLP 369
           GG GGALKYTPITKAHGVANFYG+D+VG+KVGG Q+PIS+SVFSTSGAIIDSGTVITRLP
Sbjct: 306 GGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRLP 365

Query: 370 PAAYDALKSAFQKGMTAYPKAPELSILDTCYDLSKYTSVDVPKVSFLFKGGTELDLDAIG 429
           P AY ALKSAFQKGMT YPKAPELSILDTCYDLSKYTSV++PKV FLFKGGT L+LD  G
Sbjct: 366 PDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGTG 425

Query: 430 ILYGASPEQVCLAFAGNQDPRSVAIIGNVQQKSLQVVYDVGGGRVGFGYNGC 476
           ILYGAS  QVCLAFAGN DP SVAIIGNVQQK+LQVVYDVGGG++GFGYNGC
Sbjct: 426 ILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 473

[Close]

BLAST of MC11g0385 vs. ExPASy TrEMBL
Match: A0A6J1FDU3 (aspartyl protease family protein At5g10770-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443184 PE=3 SV=1)

HSP 1 Score: 761 bits (1965), Expect = 1.33e-274
Identity = 388/472 (82.20%), Postives = 422/472 (89.41%), Query Frame = 0


Query: 10  SLLFF---FLFFHSFAGKVAPDGHHLTVELAALLPSAACSRRSAKVTGVKKEDSSPSLEV 69
           S+LFF   FLFF+S AGKV+PD H LTVELA LLPSAAC+RRS     V+   +  SLEV
Sbjct: 6   SMLFFAFCFLFFYSSAGKVSPDAHRLTVELADLLPSAACNRRS-----VQGRRTQSSLEV 65

Query: 70  VHRHGPCAGA-SKAPSEAEIFVRDQARVDLIHSKFSEESGSGSVQRIRPSKATKIPAKSG 129
           +HRHGPC G  SKAP+ AE+FV+DQARVD IHSKFS +  S  + R+RPSKATKIPAKSG
Sbjct: 66  IHRHGPCGGGESKAPTAAELFVQDQARVDFIHSKFSGDFKS--IDRLRPSKATKIPAKSG 125

Query: 130 ATIGSGNYIVSVGLGTPKKELSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSKSTSYS 189
           ATIGSGNYIV+V LGTPKK LSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPS+ST+YS
Sbjct: 126 ATIGSGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSQSTTYS 185

Query: 190 NISCSSPSCSQLESGTGNEPGCSSATKSCIYGIQYGDQSFSVGYFAKETLSLSSYDVIEN 249
           NI+CSSP CSQLESGTGN+PGCS+A KSCIYGIQYGDQSFSVGYFAKETL+L+  DVI N
Sbjct: 186 NITCSSPICSQLESGTGNQPGCSAA-KSCIYGIQYGDQSFSVGYFAKETLTLTPTDVISN 245

Query: 250 FLFGCGQNNRGLFGSAAGLLGLGQDKISIVKQTAQKYGQIFSYCLPKTASSTGYLTFGG- 309
           FLFGCGQNNRGLFGSAAGL+GLGQDKISIVKQTAQKYGQIFSYCLPKT+SSTGYL FGG 
Sbjct: 246 FLFGCGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGGS 305

Query: 310 GGNGGALKYTPITKAHGVANFYGLDVVGIKVGGTQLPISSSVFSTSGAIIDSGTVITRLP 369
           GG GGALKYTPITKAHGVANFYG+D+VG+KVGG Q+PIS+SVFSTSGAIIDSGTVITRLP
Sbjct: 306 GGGGGALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRLP 365

Query: 370 PAAYDALKSAFQKGMTAYPKAPELSILDTCYDLSKYTSVDVPKVSFLFKGGTELDLDAIG 429
           P AY ALKSAFQKGMT YPKAPELSILDTCYDLSKYTSV++PKV FLFKGGT L+LD  G
Sbjct: 366 PDAYSALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGTG 425

Query: 430 ILYGASPEQVCLAFAGNQDPRSVAIIGNVQQKSLQVVYDVGGGRVGFGYNGC 476
           ILYGAS  QVCLAFAGN DP SVAIIGNVQQK+LQVVYDVGGG++GFGYNGC
Sbjct: 426 ILYGASTTQVCLAFAGNMDPSSVAIIGNVQQKTLQVVYDVGGGKIGFGYNGC 469

[Close]

BLAST of MC11g0385 vs. ExPASy TrEMBL
Match: A0A6J1IL31 (aspartyl protease family protein At5g10770-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477209 PE=3 SV=1)

HSP 1 Score: 758 bits (1957), Expect = 2.56e-273
Identity = 384/468 (82.05%), Postives = 420/468 (89.74%), Query Frame = 0


Query: 13  FFFLFFHSFAGKVAPDGHHLTVELAALLPSAACSRRS--AKVTGVKKEDSSPSLEVVHRH 72
           F F FFHS AGKV+PD H LTVELA LLPSAAC+RRS  A+++G + + S   LEV+HRH
Sbjct: 12  FCFFFFHSSAGKVSPDAHRLTVELADLLPSAACNRRSVQAQISGGRTQSS---LEVIHRH 71

Query: 73  GPCA-GASKAPSEAEIFVRDQARVDLIHSKFSEESGSGSVQRIRPSKATKIPAKSGATIG 132
           GPC  G SKAP+ AE+FV+DQARVD IHSKFS +  S  + R+RPSKATKIPAKSGATIG
Sbjct: 72  GPCGDGNSKAPTAAELFVQDQARVDFIHSKFSGDFKS--IDRLRPSKATKIPAKSGATIG 131

Query: 133 SGNYIVSVGLGTPKKELSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSKSTSYSNISC 192
           SGNYIV+V LGTPKK LSLIFDTGSDLTWTQC PCARYCYSQKDPVFAPS+ST+YSNI+C
Sbjct: 132 SGNYIVNVALGTPKKYLSLIFDTGSDLTWTQCAPCARYCYSQKDPVFAPSQSTTYSNITC 191

Query: 193 SSPSCSQLESGTGNEPGCSSATKSCIYGIQYGDQSFSVGYFAKETLSLSSYDVIENFLFG 252
           SSP CSQLESGTGN+PGCS+A KSCIYGIQYGDQSFSVGYFAKETL+L+  DVI NFLFG
Sbjct: 192 SSPICSQLESGTGNQPGCSAA-KSCIYGIQYGDQSFSVGYFAKETLTLTPTDVISNFLFG 251

Query: 253 CGQNNRGLFGSAAGLLGLGQDKISIVKQTAQKYGQIFSYCLPKTASSTGYLTFGG-GGNG 312
           CGQNNRGLFGSAAGL+GLGQDKISIVKQTAQKYGQIFSYCLPKT+SSTGYL FGG GG G
Sbjct: 252 CGQNNRGLFGSAAGLIGLGQDKISIVKQTAQKYGQIFSYCLPKTSSSTGYLAFGGSGGGG 311

Query: 313 GALKYTPITKAHGVANFYGLDVVGIKVGGTQLPISSSVFSTSGAIIDSGTVITRLPPAAY 372
           GALKYTPITKAHGVANFYG+D+VG+KVGG Q+PIS+SVFSTSGAIIDSGTVITRLPP AY
Sbjct: 312 GALKYTPITKAHGVANFYGVDIVGMKVGGIQIPISASVFSTSGAIIDSGTVITRLPPDAY 371

Query: 373 DALKSAFQKGMTAYPKAPELSILDTCYDLSKYTSVDVPKVSFLFKGGTELDLDAIGILYG 432
            ALKSAFQKGMT YPKAPELSILDTCYDLSKYTSV++PKV FLFKGGT L+LD  GILYG
Sbjct: 372 SALKSAFQKGMTKYPKAPELSILDTCYDLSKYTSVEIPKVDFLFKGGTVLELDGTGILYG 431

Query: 433 ASPEQVCLAFAGNQDPRSVAIIGNVQQKSLQVVYDVGGGRVGFGYNGC 476
           AS  QVCLAFAGN DP SVAIIGNVQQK++QVVYDVGGG++GFGYNGC
Sbjct: 432 ASTTQVCLAFAGNLDPSSVAIIGNVQQKTMQVVYDVGGGKIGFGYNGC 473

[Close]

BLAST of MC11g0385 vs. TAIR 10
Match: AT5G10770.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 496.9 bits (1278), Expect = 1.8e-140
Identity = 255/453 (56.29%), Postives = 325/453 (71.74%), Query Frame = 0


Query: 28  DGHHLTVELAALLPSAACSRRSAKVTGVKKEDSSPSLEVVHRHGPCA----GASKAPSEA 87
           D H  T+++++LLPS++    S+ V   +   +  SL V HRHG C+    G + +P   
Sbjct: 32  DSH--TIQVSSLLPSSS----SSCVLSPRASTTKSSLHVTHRHGTCSRLNNGKATSPDHV 91

Query: 88  EIFVRDQARVDLIHSKFSEESGSGSVQRIRPSKATKIPAKSGATIGSGNYIVSVGLGTPK 147
           EI   DQARV+ IHSK S++  +  V     SK+T +PAK G+T+GSGNYIV+VGLGTPK
Sbjct: 92  EILRLDQARVNSIHSKLSKKLATDHVSE---SKSTDLPAKDGSTLGSGNYIVTVGLGTPK 151

Query: 148 KELSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSKSTSYSNISCSSPSCSQLESGTGN 207
            +LSLIFDTGSDLTWTQC+PC R CY QK+P+F PSKSTSY N+SCSS +C  L S TGN
Sbjct: 152 NDLSLIFDTGSDLTWTQCQPCVRTCYDQKEPIFNPSKSTSYYNVSCSSAACGSLSSATGN 211

Query: 208 EPGCSSATKSCIYGIQYGDQSFSVGYFAKETLSLSSYDVIENFLFGCGQNNRGLFGSAAG 267
              CS++  +CIYGIQYGDQSFSVG+ AKE  +L++ DV +   FGCG+NN+GLF   AG
Sbjct: 212 AGSCSAS--NCIYGIQYGDQSFSVGFLAKEKFTLTNSDVFDGVYFGCGENNQGLFTGVAG 271

Query: 268 LLGLGQDKISIVKQTAQKYGQIFSYCLPKTASSTGYLTFGGGGNGGALKYTPITKAHGVA 327
           LLGLG+DK+S   QTA  Y +IFSYCLP +AS TG+LTFG  G   ++K+TPI+      
Sbjct: 272 LLGLGRDKLSFPSQTATAYNKIFSYCLPSSASYTGHLTFGSAGISRSVKFTPISTITDGT 331

Query: 328 NFYGLDVVGIKVGGTQLPISSSVFSTSGAIIDSGTVITRLPPAAYDALKSAFQKGMTAYP 387
           +FYGL++V I VGG +LPI S+VFST GA+IDSGTVITRLPP AY AL+S+F+  M+ YP
Sbjct: 332 SFYGLNIVAITVGGQKLPIPSTVFSTPGALIDSGTVITRLPPKAYAALRSSFKAKMSKYP 391

Query: 388 KAPELSILDTCYDLSKYTSVDVPKVSFLFKGGTELDLDAIGILYGASPEQVCLAFAGNQD 447
               +SILDTC+DLS + +V +PKV+F F GG  ++L + GI Y     QVCLAFAGN D
Sbjct: 392 TTSGVSILDTCFDLSGFKTVTIPKVAFSFSGGAVVELGSKGIFYVFKISQVCLAFAGNSD 451

Query: 448 PRSVAIIGNVQQKSLQVVYDVGGGRVGFGYNGC 477
             + AI GNVQQ++L+VVYD  GGRVGF  NGC
Sbjct: 452 DSNAAIFGNVQQQTLEVVYDGAGGRVGFAPNGC 473

[Close]

BLAST of MC11g0385 vs. TAIR 10
Match: AT5G10760.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 423.7 bits (1088), Expect = 2.0e-118
Identity = 225/447 (50.34%), Postives = 296/447 (66.22%), Query Frame = 0


Query: 33  TVELAALLPSAACSRRSAKVTGVKKEDSSPSLEVVHRHGPCAGASK--APSEAEIFVRDQ 92
           T+++++L PS++    S+K +  K      SL VVH HG C+  S        EI  RDQ
Sbjct: 39  TIQVSSLFPSSSSCVPSSKASNTKS-----SLRVVHMHGACSHLSSDARVDHDEIIRRDQ 98

Query: 93  ARVDLIHSKFSEESGSGSVQRIRPSKATKIPAKSGATIGSGNYIVSVGLGTPKKELSLIF 152
           ARV+ I+SK S+ S +     +  +K+T++PAKSG T+GSGNYIV++G+GTPK +LSL+F
Sbjct: 99  ARVESIYSKLSKNSAN----EVSEAKSTELPAKSGITLGSGNYIVTIGIGTPKHDLSLVF 158

Query: 153 DTGSDLTWTQCEPCARYCYSQKDPVFAPSKSTSYSNISCSSPSCSQLESGTGNEPGCSSA 212
           DTGSDLTWTQCEPC   CYSQK+P F PS S++Y N+SCSSP C   ES       CS++
Sbjct: 159 DTGSDLTWTQCEPCLGSCYSQKEPKFNPSSSSTYQNVSCSSPMCEDAES-------CSAS 218

Query: 213 TKSCIYGIQYGDQSFSVGYFAKETLSLSSYDVIENFLFGCGQNNRGLFGSAAGLLGLGQD 272
             +C+Y I YGD+SF+ G+ AKE  +L++ DV+E+  FGCG+NN+GLF   AGLLGLG  
Sbjct: 219 --NCVYSIVYGDKSFTQGFLAKEKFTLTNSDVLEDVYFGCGENNQGLFDGVAGLLGLGPG 278

Query: 273 KISIVKQTAQKYGQIFSYCLPK-TASSTGYLTFGGGGNGGALKYTPITKAHGVANFYGLD 332
           K+S+  QT   Y  IFSYCLP  T++STG+LTFG  G   ++K+TPI+      N YG+D
Sbjct: 279 KLSLPAQTTTTYNNIFSYCLPSFTSNSTGHLTFGSAGISESVKFTPISSFPSAFN-YGID 338

Query: 333 VVGIKVGGTQLPISSSVFSTSGAIIDSGTVITRLPPAAYDALKSAFQKGMTAYPKAPELS 392
           ++GI VG  +L I+ + FST GAIIDSGTV TRLP   Y  L+S F++ M++Y       
Sbjct: 339 IIGISVGDKELAITPNSFSTEGAIIDSGTVFTRLPTKVYAELRSVFKEKMSSYKSTSGYG 398

Query: 393 ILDTCYDLSKYTSVDVPKVSFLFKGGTELDLDAIGILYGASPEQVCLAFAGNQDPRSVAI 452
           + DTCYD +   +V  P ++F F G T ++LD  GI       QVCLAFAGN D    AI
Sbjct: 399 LFDTCYDFTGLDTVTYPTIAFSFAGSTVVELDGSGISLPIKISQVCLAFAGNDD--LPAI 458

Query: 453 IGNVQQKSLQVVYDVGGGRVGFGYNGC 477
            GNVQQ +L VVYDV GGRVGF  NGC
Sbjct: 459 FGNVQQTTLDVVYDVAGGRVGFAPNGC 464

[Close]

BLAST of MC11g0385 vs. TAIR 10
Match: AT1G79720.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 298.1 bits (762), Expect = 1.2e-80
Identity = 190/494 (38.46%), Postives = 269/494 (54.45%), Query Frame = 0


Query: 2   AALSLSSFSLLFFFLFFHSFAGKVAPDGHHLTVELAALLP------SAACSRRSAKVTGV 61
           A L+LS   LL  FLF  S       +   L+V      P      S +C  RS     +
Sbjct: 5   ATLNLSLAPLLLVFLFLLSCVVHGVDEKKILSVHNNIWSPKKSYEASTSCFSRS-----L 64

Query: 62  KKEDSSPSLEVVHRHGPCAGAS--KAPSEAEIFVRDQARVDLIHSKFSEESGSGSVQRIR 121
            K   S +LE+ HR   C+G +           V D  RV  +  K    + S + Q + 
Sbjct: 65  GKGRESTTLEMKHRE-LCSGKTIDLGKKMRRALVLDNIRVQSLQLKIKAMTSSTTEQSV- 124

Query: 122 PSKATKIPAKSGATIGSGNYIVSVGLGTPKKELSLIFDTGSDLTWTQCEPCARYCYSQKD 181
               T+IP  SG  + S NYIV+V LG   K +SLI DTGSDLTW QC+PC R CY+Q+ 
Sbjct: 125 --SETQIPLTSGIKLESLNYIVTVELG--GKNMSLIVDTGSDLTWVQCQPC-RSCYNQQG 184

Query: 182 PVFAPSKSTSYSNISCSSPSCSQLESGTGNEPGCSS----ATKSCIYGIQYGDQSFSVGY 241
           P++ PS S+SY  + C+S +C  L + T N   C          C Y + YGD S++ G 
Sbjct: 185 PLYDPSVSSSYKTVFCNSSTCQDLVAATSNSGPCGGNNGVVKTPCEYVVSYGDGSYTRGD 244

Query: 242 FAKETLSLSSYDVIENFLFGCGQNNRGLFGSAAGLLGLGQDKISIVKQTAQKYGQIFSYC 301
            A E++ L     +ENF+FGCG+NN+GLFG ++GL+GLG+  +S+V QT + +  +FSYC
Sbjct: 245 LASESILLGD-TKLENFVFGCGRNNKGLFGGSSGLMGLGRSSVSLVSQTLKTFNGVFSYC 304

Query: 302 LPKTA-SSTGYLTFGGGG----NGGALKYTPITKAHGVANFYGLDVVGIKVGGTQLPISS 361
           LP     ++G L+FG       N  ++ YTP+ +   + +FY L++ G  +GG +L  SS
Sbjct: 305 LPSLEDGASGSLSFGNDSSVYTNSTSVSYTPLVQNPQLRSFYILNLTGASIGGVELKSSS 364

Query: 362 SVFSTSGAIIDSGTVITRLPPAAYDALKSAFQKGMTAYPKAPELSILDTCYDLSKYTSVD 421
                 G +IDSGTVITRLPP+ Y A+K  F K  + +P AP  SILDTC++L+ Y  + 
Sbjct: 365 ---FGRGILIDSGTVITRLPPSIYKAVKIEFLKQFSGFPTAPGYSILDTCFNLTSYEDIS 424

Query: 422 VPKVSFLFKGGTELDLDAIGILYGASPEQ--VCLAFAGNQDPRSVAIIGNVQQKSLQVVY 477
           +P +  +F+G  EL++D  G+ Y   P+   VCLA A       V IIGN QQK+ +V+Y
Sbjct: 425 IPIIKMIFQGNAELEVDVTGVFYFVKPDASLVCLALASLSYENEVGIIGNYQQKNQRVIY 482

[Close]

BLAST of MC11g0385 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 284.3 bits (726), Expect = 1.8e-76
Identity = 181/486 (37.24%), Postives = 263/486 (54.12%), Query Frame = 0


Query: 9   FSLLFFFLFFHSFAG-----KVAPDGHHL--TVELAALLPSAACSRRSAKVTGVKKEDSS 68
           FSL FFFL   SF+       + P+ H L     ++    S + S   ++       +SS
Sbjct: 10  FSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSFQPDSDSESLLESEFESGSDSESS 69

Query: 69  PSLEVVHRHGPCAGASKAPSE--AEIFVRDQARVDLIHSKFSEESGSGSVQRIRPSKATK 128
            S+ +   H     ++K P E  +    RD  RV  I +  ++  G       RP   + 
Sbjct: 70  SSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPRPGGFSS 129

Query: 129 IPAKSGATIGSGNYIVSVGLGTPKKELSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPS 188
               SG + GSG Y   +G+GTP + + ++ DTGSD+ W QC PC R CYSQ DP+F P 
Sbjct: 130 -SVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRR-CYSQSDPIFDPR 189

Query: 189 KSTSYSNISCSSPSCSQLESGTGNEPGCSSATKSCIYGIQYGDQSFSVGYFAKETLSLSS 248
           KS +Y+ I CSSP C +L+S      GC++  K+C+Y + YGD SF+VG F+ ETL+   
Sbjct: 190 KSKTYATIPCSSPHCRRLDSA-----GCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRR 249

Query: 249 YDVIENFLFGCGQNNRGLFGSAAGLLGLGQDKISIVKQTAQKYGQIFSYCLPKTASST-- 308
            + ++    GCG +N GLF  AAGLLGLG+ K+S   QT  ++ Q FSYCL   ++S+  
Sbjct: 250 -NRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKP 309

Query: 309 GYLTFGGGGNGGALKYTPITKAHGVANFYGLDVVGIKVGGTQLP-ISSSVF-----STSG 368
             + FG        ++TP+     +  FY + ++GI VGGT++P +++S+F        G
Sbjct: 310 SSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGG 369

Query: 369 AIIDSGTVITRLPPAAYDALKSAFQKGMTAYPKAPELSILDTCYDLSKYTSVDVPKVSFL 428
            IIDSGT +TRL   AY A++ AF+ G     +AP+ S+ DTC+DLS    V VP V   
Sbjct: 370 VIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLH 429

Query: 429 FKGGTELDLDAIGILYGASPE-QVCLAFAGNQDPRSVAIIGNVQQKSLQVVYDVGGGRVG 477
           F+ G ++ L A   L       + C AFAG      ++IIGN+QQ+  +VVYD+   RVG
Sbjct: 430 FR-GADVSLPATNYLIPVDTNGKFCFAFAGTMG--GLSIIGNIQQQGFRVVYDLASSRVG 484

[Close]

BLAST of MC11g0385 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 263.5 bits (672), Expect = 3.4e-70
Identity = 148/360 (41.11%), Postives = 204/360 (56.67%), Query Frame = 0


Query: 124 SGATIGSGNYIVSVGLGTPKKELSLIFDTGSDLTWTQCEPCARYCYSQKDPVFAPSKSTS 183
           SG   GSG Y V +G+G+P ++  ++ D+GSD+ W QC+PC + CY Q DPVF P+KS S
Sbjct: 122 SGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPC-KLCYKQSDPVFDPAKSGS 181

Query: 184 YSNISCSSPSCSQLESGTGNEPGCSSATKSCIYGIQYGDQSFSVGYFAKETLSLSSYDVI 243
           Y+ +SC S  C ++E+      GC S    C Y + YGD S++ G  A ETL+ +   V+
Sbjct: 182 YTGVSCGSSVCDRIENS-----GCHSG--GCRYEVMYGDGSYTKGTLALETLTFAK-TVV 241

Query: 244 ENFLFGCGQNNRGLFGSAAGLLGLGQDKISIVKQTAQKYGQIFSYCL-PKTASSTGYLTF 303
            N   GCG  NRG+F  AAGLLG+G   +S V Q + + G  F YCL  +   STG L F
Sbjct: 242 RNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDSTGSLVF 301

Query: 304 GGGGNGGALKYTPITKAHGVANFYGLDVVGIKVGGTQLPISSSVFSTS-----GAIIDSG 363
           G         + P+ +     +FY + + G+ VGG ++P+   VF  +     G ++D+G
Sbjct: 302 GREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTG 361

Query: 364 TVITRLPPAAYDALKSAFQKGMTAYPKAPELSILDTCYDLSKYTSVDVPKVSFLFKGGTE 423
           T +TRLP AAY A +  F+      P+A  +SI DTCYDLS + SV VP VSF F  G  
Sbjct: 362 TAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPV 421

Query: 424 LDLDAIGILYGASPE-QVCLAFAGNQDPRSVAIIGNVQQKSLQVVYDVGGGRVGFGYNGC 477
           L L A   L         C AFA +  P  ++IIGN+QQ+ +QV +D   G VGFG N C
Sbjct: 422 LTLPARNFLMPVDDSGTYCFAFAAS--PTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

The following BLAST results are available for this feature:

BLAST of MC11g0385 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8S9J6	2.6e-139	56.29	Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At...	[more]
Q9LEW3	2.7e-117	50.34	Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1	[more]
Q9LNJ3	2.6e-75	37.24	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q9LHE3	4.8e-69	41.11	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LS40	2.4e-65	40.38	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]

BLAST of MC11g0385 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022138035.1	0.0	100.00	aspartyl protease family protein At5g10770-like isoform X1 [Momordica charantia]	[more]
XP_022138043.1	0.0	99.58	aspartyl protease family protein At5g10770-like isoform X2 [Momordica charantia]	[more]
XP_022936652.1	1.12e-274	82.20	aspartyl protease family protein At5g10770-like isoform X1 [Cucurbita moschata] ...	[more]
XP_022936653.1	2.76e-274	82.20	aspartyl protease family protein At5g10770-like isoform X2 [Cucurbita moschata]	[more]
KAG7024399.1	6.45e-274	81.99	Aspartyl protease family protein, partial [Cucurbita argyrosperma subsp. argyros...	[more]

BLAST of MC11g0385 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C8A9	0.0	100.00	aspartyl protease family protein At5g10770-like isoform X1 OS=Momordica charanti...	[more]
A0A6J1CBZ3	0.0	99.58	aspartyl protease family protein At5g10770-like isoform X2 OS=Momordica charanti...	[more]
A0A6J1F908	5.41e-275	82.20	aspartyl protease family protein At5g10770-like isoform X1 OS=Cucurbita moschata...	[more]
A0A6J1FDU3	1.33e-274	82.20	aspartyl protease family protein At5g10770-like isoform X2 OS=Cucurbita moschata...	[more]
A0A6J1IL31	2.56e-273	82.05	aspartyl protease family protein At5g10770-like isoform X1 OS=Cucurbita maxima O...	[more]

BLAST of MC11g0385 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G10770.1	1.8e-140	56.29	Eukaryotic aspartyl protease family protein	[more]
AT5G10760.1	2.0e-118	50.34	Eukaryotic aspartyl protease family protein	[more]
AT1G79720.1	1.2e-80	38.46	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	1.8e-76	37.24	Eukaryotic aspartyl protease family protein	[more]
AT3G20015.1	3.4e-70	41.11	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 352..363 score: 40.45 coord: 448..463 score: 31.25 coord: 139..159 score: 43.71
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 54..476
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 108..303 e-value: 1.1E-51 score: 177.5
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 308..476 e-value: 1.1E-45 score: 157.6
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 127..476
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 133..304 e-value: 6.4E-53 score: 179.6
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 326..471 e-value: 1.4E-30 score: 106.2
None	No IPR available	PANTHER	PTHR13683:SF750	ASPARTYL PROTEASE AED1	coord: 54..476
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 148..159
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 133..472 score: 41.612976
IPR033873	CND41-like	CDD	cd05472	cnd41_like	coord: 132..476 e-value: 1.58459E-138 score: 398.568

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC11g0385.1	MC11g0385.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity