MC11g0266 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC11g0266
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptioncytochrome P450 81D1-like
LocationMC11: 2132838 .. 2136855 (+)
RNA-Seq ExpressionMC11g0266
SyntenyMC11g0266
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CGCAATTTTCCATTTATTTATTAATTTCTGATTTCACTGTGACTACAAGCAGAATATTTAAGTAGAACCAAAGATTCAAACGATGCATAGCAAAATATTGGCAGCCATGGGAGCAATCTTTTTATACTTCCCACTGTTCTTAGTGTTGTATATATTAACGGAGCACTTCCTCCACAAGATCAGAAACCTGCCGCCGACCCCTTTCCCCTCGCTCCCACTCATCGGCCATCTCTACCTCTTGAACAAACCCATCTACCGGACTCTCGCCGACATCTCGAACCGGCACGGTCCGGTTGTGTTTCTCCAGTTCGGCTCCCGGCCGGTTCTCGTGGTGTCGTCCCCTTCCGTAGCCGAAGAATGCCTGACCAAGAACGACGTCGTGTTCGCCAACCGCCCCCGTCTCCTCATATCCAAGTGCTTCGGGTACAACAACACGAATCTCGTGTGGTCCTCGTATGGGGACCACTGGCGCAGCCTCCGCCGAATCTCCACCGTTGAAGTCCTGTCCACCCACCGGCTCCAGATTCTCTCCACCGTCCGTCTGGAGGAGGTGAGGTCCTTGATTCGGAAGCTCGCAAAAAGTGAAAACCAGGACAAAATTCTCACCATGAGGCATTCGTTTTTCGATCTGACTTTCAACGTGATGCTGAGGATGATCGCCGGAAAGCGATTCTACGGCGATGACGTGGACGACGCCGACGAGGATGCGAAGATATTCAGGGAGTTGCAGGCGGAATCGACTCGATTGACCGGCATATCAAACATTGGAGATTTTGTGCCGATGATGACGTGGCTGGGATTTGGATGGAGCGTGGAGAAGAAGATGATGGATTGCCACTACAAGAGAGACGCGTTTATGCAGACTTTGATCGAGGAGCAACGGAAAAGATCAACGGCTGAAACTGAAAGTGAAATTGATGATTCGTTTCGCGATGGAAGGAGGAAGACGATGATTGAAGTGCTGCTGGAACTGCAAGAATCGGAACCTGACCACTACACCGACGAATCGATTAAAGCCTTGATGCTTGTAATTCTCTCTCTCTCTCACTCTCATTTACTTCATGTTCATTCTCGCATTATAGGTTTAACTTACCAAGTTGTTTCACAAACTTTCAACTGCTCTCTCAATTTAATTTCTATATTTTAACAATATTTGTTTTGGTATTTATTTTTATGATTTTAAAACTTCAATTCAGTCCAATTTGGTCTCTCCGAGTTAGGTTTAGTTTCAAATTTCACTCCATGATTTCTTTGAAGTCTCATAAGTCTTTCATAATTTCTTTCATTCTTTCTTTCTTTTTTTTTTTTTTTATTACAAAGTCTTTCATAAGATTGTTTTCTATTGATCACGTGAAAAAATCCTTTTTGTTTTTTGCTTGTTACTATTTACTTAGTCAATCCTAATACAATAATTGAGTAGACAAATATAAAAAAAAAAAAATTGCTCATTACTGCATAAACACATGTTTTCAATTTGATATAAGCAAATATGATTGGTGTTGAAGTTTGACTCAACTATGAATGCTAAAACTAAAGGACCAAATTTGTAATTTAGTTAAAAGTTTTGATGTATTCCTGGGCTTTGAGGCACCTTGTATTTTAAACCATTTTTTTAAAATAAGTTTTTATTATTAAACTTATCATCTTTTTTAGTACAAAAAATTTAAGGAGGGTGTGGACAGAACTTTATTGAGCTATACTTATTAAATTAGTTATAATTAACACTTACTTTATGTACTGATATAGATTTAGATTATTTAAGAATTATCTTGTAAGTTGATTTTTTATAAGTTTAACTTTTTTTTTTGGTGTAAGGGTAAATATCTAGAGATAAAAACATTAATATATACCAATTTCATATTTCTTTTAAATAATAGAGATTAAATACAGACCATCCAAAAAATATAGAGAACTATAGAAAACTTTTTTAACTACATATATAAATAGACACTTTTGAAAGTTTATAATGAAACTTAAAATTTAACCGATATAAAAACAAATATAAATTACAAAAAATCAAATAATACGATATTGTGAAGATGTGATCGGTTATATCATATTAATCTTGTCTCGTAGTGGTGGCTTTGGGTTTAAATTATTATATAAGGACCTTCTGGATCGCTTACATTTGTTAATTTTAATTATTGAATTTTTAAAATATAATATTTAGTTGAGTAAACCACCACTCAACACAAAAGTTTAAGCTAATAGGTTGGTAAATTTAACTCATTTATAAGTATCTCTAACACTATTTATTTTTATCTTATTTATTTCTACCATAATTTTAGTATAATAACACTTCACTTAATAAAATTAATTAAAATGTATCAACCACAAATTTGTATTCAAAAGTTTTTATCGTATATAAAATTTACAAATATGTGACAAAATGAGTACTTATAAGTACAAAAACCAGAAGTAATAATACCATGAATACTTATCAATACAAAAATCAAAAATAAAATTTTAAAAGTAAAAAGACTAAAATTAATAAACATTAAATACGAATCATTTCTGAAACAAAGTCAAAATAGATGAGCAAAATATAATTTGCACCTATTATTTTTATACATAATTATTGATTAGCTGTTGTCTTTCAGAAAGAAGACGGACTCATAAAATATATTTTAGGCAATTGAAGTAATTTAGTGGCATACGAGCAACAAACAGTCTTTCGGATTTTCGTAATAAACAATTTGTATTTTGAATAAATAAATATTATAATATTCCTACATTTTTCAATGAATTAATAATGCGGTTGGAAAAAACATTTTTTTTTGCTCCATATTTAATTGTTTGATTAGAATATGGTCCATTATTTTATTAAAAATATAAACTCACAATCCTTTAGATTTGGCCCCAAATCGACTTAATAATAACTCATCCTTTCAAATGTGAAAGCACCATTTTTTAATTTAAAACTCTCCTATGAAAACCTTTTAGAGGTATAGAAGTTCTTCAGGAGAAAACTTTTTAATTTTAAAACTTTAAATTAAATGATAGACTGAAATATATTTACTGTAAATATATCAAAGTATATTTCAAGTATAAAATAAGAAAAACCTTGTTAGACTGTATTACTTTGTCAAATATCTTTTATTATCCTATAGTTAAATAAAAGAACTATTTAAGTATAAATAAAAATAATTATAGATTTTACATTGATAAAAAAAAAAGTTAAAAAATTGAATTCTATAGTCAGATGTTATTGATAAGAAAAAAAAATACACACATATTTAACATTTTATTCTGCATATGGATAGGTTCTATTAACGGCAGGTACGGAGACCTCTGTGAACACCATGGAGTGGGCCCTATCTCTTCTACTGAACCATCCAGAAGTTTTAAAGAAGGCGCAGGTCGAAATTGATGATCAAGTTGGCCAGGACCGTCTGATTGACGAATCAGACATGGCCCACCTTCCTTACTTACGTGGCATAATCAACGAGACCTTCAGAATGTACCCTCCGGCTCCACTAATGGTACCTCACCAATCATCAGAAGACTGCAGTGTCGGAGGGCACCACATCCCACGTGGGACTATGCTATACGTCAATCTATGGGCCATCCACAACGACCCCAAGATTTGGGCCAACCCGACAAAGTTTGATCCCGACAGGTTTGACAGGCTGGAAAGTGACAAGTACGGGTACGGGTTCAATCTGATGCCGTTCGGGTCGGGTAGAAGGGGGTGTCCAGGAGAGGGACTGGGCCTGCGGATCATTGGGCTGGTGTTGGGCTCACTGATCCAGTGCTTTGAATGGGAGCGTCCTGGTGAGAAGTTGGTTGACATGACAGAAGGCATTGCAGTCTCTATGCCCAAGGCCCAACCATTGCAGGCTAAATGCAGGCCTCGGCCCATAATTGCTAACCTTCCATCTCAAACCAAAACCAACCAGGCCCATTGATGTATGGCCCAGGGTTTAGCTTTTTAACGCAATAATAATAATAATACAATGGCGTTTATTTCCAGCATTGTTTTTTTAGTTTTCCATTTTCCTTCTTTCGACAAATCGAC

mRNA sequence

CGCAATTTTCCATTTATTTATTAATTTCTGATTTCACTGTGACTACAAGCAGAATATTTAAGTAGAACCAAAGATTCAAACGATGCATAGCAAAATATTGGCAGCCATGGGAGCAATCTTTTTATACTTCCCACTGTTCTTAGTGTTGTATATATTAACGGAGCACTTCCTCCACAAGATCAGAAACCTGCCGCCGACCCCTTTCCCCTCGCTCCCACTCATCGGCCATCTCTACCTCTTGAACAAACCCATCTACCGGACTCTCGCCGACATCTCGAACCGGCACGGTCCGGTTGTGTTTCTCCAGTTCGGCTCCCGGCCGGTTCTCGTGGTGTCGTCCCCTTCCGTAGCCGAAGAATGCCTGACCAAGAACGACGTCGTGTTCGCCAACCGCCCCCGTCTCCTCATATCCAAGTGCTTCGGGTACAACAACACGAATCTCGTGTGGTCCTCGTATGGGGACCACTGGCGCAGCCTCCGCCGAATCTCCACCGTTGAAGTCCTGTCCACCCACCGGCTCCAGATTCTCTCCACCGTCCGTCTGGAGGAGGTGAGGTCCTTGATTCGGAAGCTCGCAAAAAGTGAAAACCAGGACAAAATTCTCACCATGAGGCATTCGTTTTTCGATCTGACTTTCAACGTGATGCTGAGGATGATCGCCGGAAAGCGATTCTACGGCGATGACGTGGACGACGCCGACGAGGATGCGAAGATATTCAGGGAGTTGCAGGCGGAATCGACTCGATTGACCGGCATATCAAACATTGGAGATTTTGTGCCGATGATGACGTGGCTGGGATTTGGATGGAGCGTGGAGAAGAAGATGATGGATTGCCACTACAAGAGAGACGCGTTTATGCAGACTTTGATCGAGGAGCAACGGAAAAGATCAACGGCTGAAACTGAAAGTGAAATTGATGATTCGTTTCGCGATGGAAGGAGGAAGACGATGATTGAAGTGCTGCTGGAACTGCAAGAATCGGAACCTGACCACTACACCGACGAATCGATTAAAGCCTTGATGCTTGTTCTATTAACGGCAGGTACGGAGACCTCTGTGAACACCATGGAGTGGGCCCTATCTCTTCTACTGAACCATCCAGAAGTTTTAAAGAAGGCGCAGGTCGAAATTGATGATCAAGTTGGCCAGGACCGTCTGATTGACGAATCAGACATGGCCCACCTTCCTTACTTACGTGGCATAATCAACGAGACCTTCAGAATGTACCCTCCGGCTCCACTAATGGTACCTCACCAATCATCAGAAGACTGCAGTGTCGGAGGGCACCACATCCCACGTGGGACTATGCTATACGTCAATCTATGGGCCATCCACAACGACCCCAAGATTTGGGCCAACCCGACAAAGTTTGATCCCGACAGGTTTGACAGGCTGGAAAGTGACAAGTACGGGTACGGGTTCAATCTGATGCCGTTCGGGTCGGGTAGAAGGGGGTGTCCAGGAGAGGGACTGGGCCTGCGGATCATTGGGCTGGTGTTGGGCTCACTGATCCAGTGCTTTGAATGGGAGCGTCCTGGTGAGAAGTTGGTTGACATGACAGAAGGCATTGCAGTCTCTATGCCCAAGGCCCAACCATTGCAGGCTAAATGCAGGCCTCGGCCCATAATTGCTAACCTTCCATCTCAAACCAAAACCAACCAGGCCCATTGATGTATGGCCCAGGGTTTAGCTTTTTAACGCAATAATAATAATAATACAATGGCGTTTATTTCCAGCATTGTTTTTTTAGTTTTCCATTTTCCTTCTTTCGACAAATCGAC

Coding sequence (CDS)

ATGCATAGCAAAATATTGGCAGCCATGGGAGCAATCTTTTTATACTTCCCACTGTTCTTAGTGTTGTATATATTAACGGAGCACTTCCTCCACAAGATCAGAAACCTGCCGCCGACCCCTTTCCCCTCGCTCCCACTCATCGGCCATCTCTACCTCTTGAACAAACCCATCTACCGGACTCTCGCCGACATCTCGAACCGGCACGGTCCGGTTGTGTTTCTCCAGTTCGGCTCCCGGCCGGTTCTCGTGGTGTCGTCCCCTTCCGTAGCCGAAGAATGCCTGACCAAGAACGACGTCGTGTTCGCCAACCGCCCCCGTCTCCTCATATCCAAGTGCTTCGGGTACAACAACACGAATCTCGTGTGGTCCTCGTATGGGGACCACTGGCGCAGCCTCCGCCGAATCTCCACCGTTGAAGTCCTGTCCACCCACCGGCTCCAGATTCTCTCCACCGTCCGTCTGGAGGAGGTGAGGTCCTTGATTCGGAAGCTCGCAAAAAGTGAAAACCAGGACAAAATTCTCACCATGAGGCATTCGTTTTTCGATCTGACTTTCAACGTGATGCTGAGGATGATCGCCGGAAAGCGATTCTACGGCGATGACGTGGACGACGCCGACGAGGATGCGAAGATATTCAGGGAGTTGCAGGCGGAATCGACTCGATTGACCGGCATATCAAACATTGGAGATTTTGTGCCGATGATGACGTGGCTGGGATTTGGATGGAGCGTGGAGAAGAAGATGATGGATTGCCACTACAAGAGAGACGCGTTTATGCAGACTTTGATCGAGGAGCAACGGAAAAGATCAACGGCTGAAACTGAAAGTGAAATTGATGATTCGTTTCGCGATGGAAGGAGGAAGACGATGATTGAAGTGCTGCTGGAACTGCAAGAATCGGAACCTGACCACTACACCGACGAATCGATTAAAGCCTTGATGCTTGTTCTATTAACGGCAGGTACGGAGACCTCTGTGAACACCATGGAGTGGGCCCTATCTCTTCTACTGAACCATCCAGAAGTTTTAAAGAAGGCGCAGGTCGAAATTGATGATCAAGTTGGCCAGGACCGTCTGATTGACGAATCAGACATGGCCCACCTTCCTTACTTACGTGGCATAATCAACGAGACCTTCAGAATGTACCCTCCGGCTCCACTAATGGTACCTCACCAATCATCAGAAGACTGCAGTGTCGGAGGGCACCACATCCCACGTGGGACTATGCTATACGTCAATCTATGGGCCATCCACAACGACCCCAAGATTTGGGCCAACCCGACAAAGTTTGATCCCGACAGGTTTGACAGGCTGGAAAGTGACAAGTACGGGTACGGGTTCAATCTGATGCCGTTCGGGTCGGGTAGAAGGGGGTGTCCAGGAGAGGGACTGGGCCTGCGGATCATTGGGCTGGTGTTGGGCTCACTGATCCAGTGCTTTGAATGGGAGCGTCCTGGTGAGAAGTTGGTTGACATGACAGAAGGCATTGCAGTCTCTATGCCCAAGGCCCAACCATTGCAGGCTAAATGCAGGCCTCGGCCCATAATTGCTAACCTTCCATCTCAAACCAAAACCAACCAGGCCCATTGA

Protein sequence

MHSKILAAMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADISNRHGPVVFLQFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSYGDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFDLTFNVMLRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKKMMDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQESEPDHYTDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAHLPYLRGIINETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHNDPKIWANPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLPSQTKTNQAH
Homology
BLAST of MC11g0266 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 6.3e-139
Identity = 248/491 (50.51%), Postives = 336/491 (68.43%), Query Frame = 0

Query: 34  RNLPPTPFPSLPLIGHLYLLNKPIYRTLADISNRHGPVVFLQFGSRPVLVVSSPSVAEEC 93
           RNLPP+P  +LP+IGHL+L+ K ++R+L D+S ++G V  LQ G+R VLVVSSP+ AEEC
Sbjct: 30  RNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKYGSVFSLQLGNRLVLVVSSPAAAEEC 89

Query: 94  LTKNDVVFANRPRLLISKCFGYNNTNLVWSSYGDHWRSLRRISTVEVLSTHRLQILSTVR 153
            TKND+VFANRP  ++ K  GYN T +V S YG+HWR+LRR++ VE+ S   L    ++R
Sbjct: 90  FTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEHWRNLRRLAAVEIFSAGSLNRFLSIR 149

Query: 154 LEEVRSLIRKLAKSENQD-KILTMRHSFFDLTFNVMLRMIAGKRFYGDDVDDADEDAKIF 213
            +EV+ L+  L +S  QD   + M+    +L+FNV +RM+AGKR++G DVD   ++AK+F
Sbjct: 150 EDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNVTMRMVAGKRYFGQDVD--SDEAKLF 209

Query: 214 RELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKKMMDCHYKRDAFMQTLIEEQRKRSTA 273
           R L  E     G SN GDFVP + W+ F  + EKK+     + DAF+Q LI E R     
Sbjct: 210 RALIGEVFEHAGASNPGDFVPFLRWIDFK-NYEKKVSKISQEMDAFLQRLIHESRINK-- 269

Query: 274 ETESEIDDSFRDGRRKTMIEVLLELQESEPDHYTDESIKALMLVLLTAGTETSVNTMEWA 333
                           TMI+ LL LQES+P++YTD+ IK +++VLL AGT+TS  T+EWA
Sbjct: 270 -------------NNVTMIDHLLSLQESQPEYYTDQIIKGIIMVLLLAGTDTSAVTVEWA 329

Query: 334 LSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAHLPYLRGIINETFRMYPPAPLMVPHQ 393
           +SLLLNHPE L+KA+ EI+ QVG +RLI+E D+  L YL  II+ETFR+ P AP++VPH+
Sbjct: 330 MSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISETFRLCPAAPMLVPHE 389

Query: 394 SSEDCSVGGHHIPRGTMLYVNLWAIHNDPKIWANPTKFDPDRFDRLESDKYGYGFNLMPF 453
           SS+DC V G+ +P+GT+L VN WAIH DP+ W  PT F P+R   +E +       LMPF
Sbjct: 390 SSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERHGGVELEP----SKLMPF 449

Query: 454 GSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGEKLVDMTEGIAVSMPKAQPLQAKCRP 513
           G GRR CPG GL  R++GL LG+LIQCFEW+R GE  +DM EG  ++MPKAQPL+A C+P
Sbjct: 450 GMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLTMPKAQPLEALCKP 498

Query: 514 RPIIANLPSQT 524
           R I+  + S+T
Sbjct: 510 RNILHKVVSET 498

BLAST of MC11g0266 vs. ExPASy Swiss-Prot
Match: Q9LHA1 (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 2.1e-126
Identity = 241/516 (46.71%), Postives = 329/516 (63.76%), Query Frame = 0

Query: 9   MGAIFLYFPLFLVLYILTEHFLHKIR----NLPPTPFPSLPLIGHLYLLNKPIYRTLADI 68
           M  I+L   LF     L+   L   R    NLPP+P    P+IGHL+LL  P++R    +
Sbjct: 8   METIYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSL 67

Query: 69  SN--RHGPVVFLQFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNLVW 128
           S    +  +  L  GSR V VVSS +VAEEC TKNDVV ANRP  L+ K  GYN+T +V 
Sbjct: 68  SESLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVG 127

Query: 129 SSYGDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKI-LTMRHSFF 188
           ++YGD WR+LRRI T+E+ S+ RL    ++R +E+R LI  LAK+     + + M+  F 
Sbjct: 128 AAYGDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFM 187

Query: 189 DLTFNVMLRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGFG 248
            LT N ++RM+AGKRFYGD  ++ D +AK  R+L AE     G  N  D+ P++ ++   
Sbjct: 188 CLTINNIIRMVAGKRFYGDGTEN-DNEAKHVRQLIAEVVVSGGAGNAADYFPILRYV--- 247

Query: 249 WSVEKKMMDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQESE 308
            + EK +     + D F+Q+L+ E+R                  +  TMI+ LL LQE++
Sbjct: 248 TNYEKHVKKLAGRVDEFLQSLVNEKRVEKV--------------KGNTMIDHLLSLQETQ 307

Query: 309 PDHYTDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLID 368
           PD+YTD  IK ++LV++ AGT+TS  T+EWA+S LLNHPEVL+KA+ EIDDQ+G DRL++
Sbjct: 308 PDYYTDVIIKGIILVMILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVE 367

Query: 369 ESDMAHLPYLRGIINETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHNDP 428
           E D+  LPYL+ I++ET R+YP AP+++PH +SEDC V G+ +PRGT++ VN WAIH DP
Sbjct: 368 EQDIVKLPYLQHIVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDP 427

Query: 429 KIWANPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFE 488
           K+W  P KF P+RF++   DK      LMPFG GRR CPG GL  R++ L LGSL+QCFE
Sbjct: 428 KLWEEPEKFKPERFEKKGEDK-----KLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFE 487

Query: 489 WERPGEKLVDMTEG-IAVSMPKAQPLQAKCRPRPII 517
           WER  EK +DM E     +M KA  LQA C+ RPI+
Sbjct: 488 WERVEEKYLDMRESEKGTTMRKATSLQAMCKARPIV 500

BLAST of MC11g0266 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 4.7e-126
Identity = 244/518 (47.10%), Postives = 337/518 (65.06%), Query Frame = 0

Query: 12  IFLYFPLFLVLYILTEHFLH-KIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADIS----- 71
           + LY    L+  I++  FL  K +NLPP+P   LP+IGHL LL  PI+RTL   S     
Sbjct: 8   VVLYSIFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDH 67

Query: 72  NRHGPVVFLQFGSRPVLVVSSPSV-AEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSS 131
           N  G V+ L+ GSR V VVSS  V AEEC  KNDVV ANRP+++I K  GYNNTN++ + 
Sbjct: 68  NDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAP 127

Query: 132 YGDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDK-ILTMRHSFFDL 191
           YGDHWR+LRR+ T+E+ STHRL     VR +EVR LI +L++     K ++ ++    DL
Sbjct: 128 YGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDL 187

Query: 192 TFNVMLRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWS 251
           TFN ++RM+ GKR+YG++  D +E+AK  R+L A+    T   N  D+VP++       S
Sbjct: 188 TFNNIMRMMTGKRYYGEETTD-EEEAKRVRKLVADVGANTSSGNAVDYVPILRLFS---S 247

Query: 252 VEKKMMDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQESEPD 311
            E ++     + D F+Q LI+++R +    T              TMI+ LL LQ+S+ +
Sbjct: 248 YENRVKKLGEETDKFLQGLIDDKRGQQETGT--------------TMIDHLLVLQKSDIE 307

Query: 312 HYTDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDES 371
           +YTD+ IK ++L+++ AGT TS  T+EWALS LLNHP+V+ KA+ EID++VG DRLI+E+
Sbjct: 308 YYTDQIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEA 367

Query: 372 DMAHLPYLRGIINETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHNDPKI 431
           D++ LPYL+ I+ ET R++P  PL+VPH +SEDC +G + +PRGT L VN WAIH DP  
Sbjct: 368 DLSELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNT 427

Query: 432 WANPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWE 491
           W +P  F P+RF++ E  +      L+ FG GRR CPG GL  RI+GL LGSLIQCFEWE
Sbjct: 428 WDDPDSFKPERFEKEEEAQ-----KLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWE 487

Query: 492 RPGEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLPS 522
           R G   VDM EG+  ++PKA PL+A C+ RP +  + S
Sbjct: 488 RVGNVEVDMKEGVGNTVPKAIPLKAICKARPFLHKIIS 502

BLAST of MC11g0266 vs. ExPASy Swiss-Prot
Match: Q0WTF4 (Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 4.7e-126
Identity = 235/515 (45.63%), Postives = 340/515 (66.02%), Query Frame = 0

Query: 12  IFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADISNRHGPV 71
           I L   LFL+ Y L      K  NLPP+P  SLP++GH  LL  P++R    +S  HGP+
Sbjct: 6   IVLPLALFLLAYKLFFTSKTKRFNLPPSPPYSLPILGHHNLLKPPVHRLFHRLSKTHGPI 65

Query: 72  VFLQFGSRPVLVVSSPSVAEECLT-KNDVVFANRPRLLISKCFGYNNTNLVWSSYGDHWR 131
             LQFGSR  +V+SS S+A +C T +ND++ +NRP  L +K   YN T +  + YGDHWR
Sbjct: 66  FSLQFGSRRAVVISSSSLATQCFTGQNDIILSNRPCFLTAKYVAYNYTTVGTAPYGDHWR 125

Query: 132 SLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFDLTFNVMLR 191
           +LRRI ++E+LS++RL     +R +E+  ++ +L++  N++  + +     DLTFN ++R
Sbjct: 126 NLRRICSLEILSSNRLTNFLHIRKDEIHRMLTRLSRDVNKE--IELEPLLSDLTFNNIVR 185

Query: 192 MIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKKMMD 251
           M+ GKR+YGD+V + +E+A +F++L A+    +G  + GD++P M    FG S EKK+  
Sbjct: 186 MVTGKRYYGDEVHN-EEEANVFKKLVADINDCSGARHPGDYLPFMKM--FGGSFEKKVKA 245

Query: 252 CHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQESEPDHYTDESI 311
                D  +Q L+EE +               RD    TM+  LL LQ++EP++YTD +I
Sbjct: 246 LAEAMDEILQRLLEECK---------------RDKDGNTMVNHLLSLQQNEPEYYTDVTI 305

Query: 312 KALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAHLPY 371
           K LML ++ AGT+TS  T+EWA+S LLNHPE L+KA++EID+++GQ+RLIDE D+A+LPY
Sbjct: 306 KGLMLGMMIAGTDTSAVTLEWAMSSLLNHPEALEKAKLEIDEKIGQERLIDEPDIANLPY 365

Query: 372 LRGIINETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHNDPKIWANPTKF 431
           L+ I++ETFR+YP APL+VP   +ED  VGG+ +PRGTM+ VN WAIH DP++W  P KF
Sbjct: 366 LQNIVSETFRLYPAAPLLVPRSPTEDIKVGGYDVPRGTMVMVNAWAIHRDPELWNEPEKF 425

Query: 432 DPDRFDRLESDKYGYGFN-LMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGEKL 491
            P+RF+  E    G   + LMPFG+GRR CPG GLG +I+ L LGSLIQCF+W++   + 
Sbjct: 426 KPERFNGGEGGGRGEDVHKLMPFGNGRRSCPGAGLGQKIVTLALGSLIQCFDWQKVNGEA 485

Query: 492 VDMTEGIAVSMPKAQPLQAKCRPRPIIANLPSQTK 525
           +DMTE   ++M K  PL A C+ RPI++ L +  K
Sbjct: 486 IDMTETPGMAMRKKIPLSALCQSRPIMSKLQAHLK 500

BLAST of MC11g0266 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 1.0e-125
Identity = 235/504 (46.63%), Postives = 329/504 (65.28%), Query Frame = 0

Query: 14  LYFPLFLVLYILTEHF--LHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADISNRHGPV 73
           L   LF ++  L   F    K +NLPP P   LP+IG+L+ L +P++ T   +S ++G +
Sbjct: 8   LIISLFFLIITLKVFFNTSRKFKNLPPGP-QCLPIIGNLHQLKQPLHHTFHTLSQKYGQI 67

Query: 74  VFLQFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSYGDHWRS 133
             L FGSR V+VVSS ++A+EC TKND+V ANRP  L  K  GYNNT +  S YGDHWR+
Sbjct: 68  FSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRN 127

Query: 134 LRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLA-KSENQDKILTMRHSFFDLTFNVMLR 193
           LRRI ++E+LS+HRL     +R +E+  LI+KLA KS N    + +R  F ++TFN ++R
Sbjct: 128 LRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTIMR 187

Query: 194 MIAGKRFYGDDVDDAD-EDAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKKMM 253
           M++GKR+YG+D D +D E+A++FR +  E   L G +N+GDF+  + W  F   +EK++ 
Sbjct: 188 MVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFD-GLEKRLK 247

Query: 254 DCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQESEPDHYTDES 313
               + DAF+Q LI+E R              F      TMI+ LL  Q+S+P++YTD+ 
Sbjct: 248 KISKRTDAFLQGLIDEHR--------------FGKRNSNTMIDHLLTQQQSQPEYYTDQI 307

Query: 314 IKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAHLP 373
           IK LM+V+L AGT+TS  T+EWA+S LLNHPE++KKA+ E+D  +G DR +DE D++ LP
Sbjct: 308 IKGLMVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLP 367

Query: 374 YLRGIINETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHNDPKIWANPTK 433
           YL+ I+ ET R++  APL+VPH SSED S+GG++IP+ T+L VN W IH DP +W++PT 
Sbjct: 368 YLQSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTC 427

Query: 434 FDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGEKL 493
           F P+RF     +K G    L+ FG GRR CPGE L  R  GL LG LIQCFEW+R GE+ 
Sbjct: 428 FKPERF-----EKEGEVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEK 487

Query: 494 VDMTEGIAVSMPKAQPLQAKCRPR 514
           +DM E   ++  K   L A C+ R
Sbjct: 488 IDMVEAKGITAGKKTSLNAMCKVR 490

BLAST of MC11g0266 vs. NCBI nr
Match: XP_022146589.1 (cytochrome P450 81D1-like [Momordica charantia])

HSP 1 Score: 1059 bits (2738), Expect = 0.0
Identity = 526/529 (99.43%), Postives = 528/529 (99.81%), Query Frame = 0

Query: 1   MHSKILAAMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRT 60
           MHSKILAAMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRT
Sbjct: 1   MHSKILAAMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRT 60

Query: 61  LADISNRHGPVVFLQFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNL 120
           LADISNRHGPVVFL+FGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNL
Sbjct: 61  LADISNRHGPVVFLRFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNL 120

Query: 121 VWSSYGDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSF 180
           VWSSYGDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSF
Sbjct: 121 VWSSYGDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSF 180

Query: 181 FDLTFNVMLRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGF 240
           FDLTFNVMLRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGF
Sbjct: 181 FDLTFNVMLRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGF 240

Query: 241 GWSVEKKMMDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQES 300
           GWSVEKKMMDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQES
Sbjct: 241 GWSVEKKMMDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQES 300

Query: 301 EPDHYTDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLI 360
           EPDHYTDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEID+QVGQDRLI
Sbjct: 301 EPDHYTDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDBQVGQDRLI 360

Query: 361 DESDMAHLPYLRGIINETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHND 420
           DESDMAHLPYLRGII ETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHND
Sbjct: 361 DESDMAHLPYLRGIIXETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHND 420

Query: 421 PKIWANPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCF 480
           PKIWANPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCF
Sbjct: 421 PKIWANPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCF 480

Query: 481 EWERPGEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLPSQTKTNQAH 529
           EWERPGEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLPSQTKTNQAH
Sbjct: 481 EWERPGEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLPSQTKTNQAH 529

BLAST of MC11g0266 vs. NCBI nr
Match: XP_038896683.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 816 bits (2109), Expect = 5.59e-294
Identity = 401/519 (77.26%), Postives = 461/519 (88.82%), Query Frame = 0

Query: 8   AMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADISNR 67
           A+GAIFLY PLFLVLYILTEHFLHKIRNLPPTPFPS+P++GHL+LL KPIYR+L +IS+R
Sbjct: 35  AIGAIFLYIPLFLVLYILTEHFLHKIRNLPPTPFPSIPILGHLHLLKKPIYRSLYNISHR 94

Query: 68  HGPVVFLQFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSYGD 127
           +GPVVFL+ G R +L+VSSPS+AEECLTKND+VFANRPRLLISKCFGYNNTNLVWSSYGD
Sbjct: 95  YGPVVFLRLGYRSILIVSSPSIAEECLTKNDIVFANRPRLLISKCFGYNNTNLVWSSYGD 154

Query: 128 HWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFDLTFNV 187
           HWR+LRRI TVE+LSTHRL +LS VRLEEVRSLI++L K ENQ  ++ M+H FFDLTFN+
Sbjct: 155 HWRNLRRICTVEILSTHRLHMLSIVRLEEVRSLIQRLLKCENQ--LVNMKHEFFDLTFNL 214

Query: 188 MLRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKK 247
           MLRMI GKRFYGDDVDD DE AK+FRELQAESTRL+G SNIGDF+P++ WLGFG ++EK 
Sbjct: 215 MLRMIVGKRFYGDDVDDVDE-AKLFRELQAESTRLSGKSNIGDFIPLIKWLGFGSTLEKD 274

Query: 248 MMDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQESEPDHYTD 307
           MM+C  +RD  MQ+LIE+ R+R      +EIDDSFRDGR+KTMIEVLLELQESEP+ Y D
Sbjct: 275 MMNCQNRRDTLMQSLIEQHRRRRM----TEIDDSFRDGRKKTMIEVLLELQESEPEQYND 334

Query: 308 ESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAH 367
           E+I+ALML++L AGTETS + MEWALSLLLNHP +LKKAQ EID QVG +R IDESDMAH
Sbjct: 335 ETIRALMLLMLVAGTETSGSIMEWALSLLLNHPTILKKAQTEIDHQVGHERSIDESDMAH 394

Query: 368 LPYLRGIINETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHNDPKIWANP 427
           LPYLRGIINET RMYPPAPL+VPH+SSEDCSVGG+HIPRGT+LY+N+WAI NDPKIWA+P
Sbjct: 395 LPYLRGIINETLRMYPPAPLLVPHESSEDCSVGGYHIPRGTVLYINIWAIQNDPKIWAHP 454

Query: 428 TKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGE 487
            KFDPDRF+  ES+ Y   FNLMPFG GRRGCPGEGLGLR+IGLVLGSLIQCFEWERP E
Sbjct: 455 RKFDPDRFNGHESENYR--FNLMPFGLGRRGCPGEGLGLRMIGLVLGSLIQCFEWERPSE 514

Query: 488 KLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLPSQTKTN 526
           +LVDMTEGIA++MPKAQ L AKCRPRPII NL SQTK++
Sbjct: 515 QLVDMTEGIAITMPKAQHLHAKCRPRPIIVNLLSQTKSS 544

BLAST of MC11g0266 vs. NCBI nr
Match: XP_022940352.1 (cytochrome P450 81D1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 804 bits (2076), Expect = 1.49e-289
Identity = 397/522 (76.05%), Postives = 468/522 (89.66%), Query Frame = 0

Query: 9   MGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADISNRH 68
           MGAI +Y PLF VLY+LTE FLH+IRNLPPTPF SLP+IGHL+LL KP+YRTLA+ISNR+
Sbjct: 1   MGAIIVYIPLFFVLYVLTEQFLHRIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60

Query: 69  GPVVFLQFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSYGDH 128
           GPVVFL+FGSR VLVVSSPS AEECL+KNDVVFA+RPRLLISK FGYNN NLVWSSYGDH
Sbjct: 61  GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120

Query: 129 WRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFDLTFNVM 188
           WR+LRRISTVE+LSTHRLQ+L+ VRL+EV+SLI +LAK ENQ  +  M ++FFDLTFNVM
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAAVRLDEVKSLIGRLAKGENQ--VQDMNNAFFDLTFNVM 180

Query: 189 LRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKKM 248
           LRMI GKRFYG+DV+DADE A  FRELQ++ST+L G +N+GDF+P+M+WLG GW+ EK++
Sbjct: 181 LRMIVGKRFYGEDVEDADE-AHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGWAAEKEI 240

Query: 249 MDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQESEPDHYTDE 308
           M+CH KRDAFMQ LI + + R+    E+E D+SFRDGR+KT+IEVLLELQES+PD Y DE
Sbjct: 241 MECHQKRDAFMQNLINQHKNRAI---ETEPDNSFRDGRKKTLIEVLLELQESDPDQYNDE 300

Query: 309 SIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAHL 368
           +I+ALMLVLL AGTETS+NTMEWALSL+LN+PEVL+KAQ+EID +VG DRL+DESDMA++
Sbjct: 301 TIRALMLVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANV 360

Query: 369 PYLRGIINETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHNDPKIWANPT 428
           P+LRGIINET RMYPPAPLMVPH+SS+DCSVGG+HIPRGTML+VNLWAIHNDPKIWA+P 
Sbjct: 361 PFLRGIINETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPR 420

Query: 429 KFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGEK 488
           KFDPDRF ++ES+K G  F LMPFGSGRRGCPGEGLGLR+IGLVLGSLIQCFEWERPGE+
Sbjct: 421 KFDPDRFVKVESEKLG--FKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEE 480

Query: 489 LVDMTEGIAVSMPKAQPLQAKCRPRPIIANLP-SQTKTNQAH 529
           LVDMTEG+AVSMPKA+ LQAKC+PRPI+  L  SQTK+N A+
Sbjct: 481 LVDMTEGVAVSMPKARHLQAKCKPRPIVDRLHLSQTKSNLAY 514

BLAST of MC11g0266 vs. NCBI nr
Match: XP_023523281.1 (cytochrome P450 81D1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 796 bits (2056), Expect = 1.65e-286
Identity = 393/522 (75.29%), Postives = 467/522 (89.46%), Query Frame = 0

Query: 9   MGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADISNRH 68
           MGAI +Y PLFLVLY+LTE FLH+IRNLPPTPF SLP+IGHL+LL KP+YRTLA+ISNR+
Sbjct: 1   MGAIIVYIPLFLVLYVLTEQFLHRIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60

Query: 69  GPVVFLQFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSYGDH 128
           GPVVFL+FGSR VLVVSSPS AEECL+KNDVVFA+RPRLLISK FGYNN NLVWSSYGDH
Sbjct: 61  GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120

Query: 129 WRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFDLTFNVM 188
           WR+LRRISTVE+LSTHRLQ+L+ VR +EV+SLI +LA+ ENQ  +  M ++FFDLTFNVM
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAAVRSDEVKSLIGRLARGENQ--VQDMNNAFFDLTFNVM 180

Query: 189 LRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKKM 248
           LRMI GKRFYG+DV+DADE A  FRELQ++ST+L G +N+ DF+P+M+WLG GW+ EK++
Sbjct: 181 LRMIVGKRFYGEDVEDADE-AHRFRELQSKSTQLGGKTNMEDFIPLMSWLGLGWAAEKEI 240

Query: 249 MDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQESEPDHYTDE 308
           M+CH KRDAFMQ LI + + R+    E+E D+SFRDGR+KT+IEVL+ELQES+PD Y DE
Sbjct: 241 MECHQKRDAFMQNLINQHKNRAI---ETEPDNSFRDGRKKTLIEVLVELQESDPDQYNDE 300

Query: 309 SIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAHL 368
           +I+ALMLVLL AGTETS+NTMEWALSL+LN+PEVL+KAQ+EID +VG DRL+DESDMA++
Sbjct: 301 TIRALMLVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANV 360

Query: 369 PYLRGIINETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHNDPKIWANPT 428
           P+LRGIINET RMYPPAPLMVPH+SS+DCSVGG+ IPRGTML+VNLWAIHNDPKIWA+P 
Sbjct: 361 PFLRGIINETLRMYPPAPLMVPHESSKDCSVGGYQIPRGTMLFVNLWAIHNDPKIWADPR 420

Query: 429 KFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGEK 488
           KFDPDRF +++S+K+G  F LMPFGSGRRGCPGEGLGLR+IGLVLGSLIQCFEWERPGE 
Sbjct: 421 KFDPDRFVKVDSEKHG--FKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGED 480

Query: 489 LVDMTEGIAVSMPKAQPLQAKCRPRPIIANLP-SQTKTNQAH 529
           LVDMTEG+AVSMPKA+ LQAKC+PRPI+A L  SQTK+N A+
Sbjct: 481 LVDMTEGVAVSMPKARHLQAKCKPRPIVARLHLSQTKSNLAY 514

BLAST of MC11g0266 vs. NCBI nr
Match: XP_022982225.1 (cytochrome P450 81D1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 791 bits (2044), Expect = 1.10e-284
Identity = 393/522 (75.29%), Postives = 465/522 (89.08%), Query Frame = 0

Query: 9   MGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADISNRH 68
           MGAI LY PLFLVLY+LTE F+HKIRNLPPTPF SLP+IGHL+LL KP+YRTLA+ISNR+
Sbjct: 1   MGAIILYIPLFLVLYVLTEQFIHKIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60

Query: 69  GPVVFLQFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSYGDH 128
           GPVVFL+FGSR VLVVSSPS AEECL+KNDVVFA+RPRLLISK FGYNN NLVWSSYGDH
Sbjct: 61  GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120

Query: 129 WRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFDLTFNVM 188
           WR+LRRISTVE+LSTHRLQ+L+ VRL+EV+SLI +LA+ ENQ  +  M ++FFDLTFNVM
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAGVRLDEVKSLIGRLARGENQ--VQDMNNAFFDLTFNVM 180

Query: 189 LRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKKM 248
           LRMI GKRFYG+DV+DADE A  FRELQ++ST+L G +N+GDF+P+M+WLG G + EK++
Sbjct: 181 LRMIVGKRFYGEDVEDADE-AHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGRAAEKEI 240

Query: 249 MDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQESEPDHYTDE 308
           M+CH KRDAFMQ LI + + R+    E+E  + FRDGR+KT+IEVLLELQES+PD Y DE
Sbjct: 241 MECHQKRDAFMQNLINQHKNRAI---ETEPHNLFRDGRKKTLIEVLLELQESDPDQYNDE 300

Query: 309 SIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAHL 368
           +I+ALMLVLL AGTETS+NTMEWALSL+LN+PEVL+KAQ+EID +VG DRL+DESDMA++
Sbjct: 301 TIRALMLVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANV 360

Query: 369 PYLRGIINETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHNDPKIWANPT 428
           P+LRGIINET RMYPPAPLMVPH+SS+DCSVGG+HIPRGTML+VNLWAIHNDPKIWA+P 
Sbjct: 361 PFLRGIINETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPR 420

Query: 429 KFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGEK 488
           KFDPDRF +++S+K G  F L+PFGSGRRGCPGEGLGLR+IGLVLGSLIQCFEWERPGE 
Sbjct: 421 KFDPDRFVKVDSEKLG--FKLLPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGED 480

Query: 489 LVDMTEGIAVSMPKAQPLQAKCRPRPIIANLP-SQTKTNQAH 529
           LVDMTEG+AVSMPKA+ LQAKC+PRPI+  L  SQTK+N A+
Sbjct: 481 LVDMTEGVAVSMPKARHLQAKCKPRPIVDRLHLSQTKSNLAY 514

BLAST of MC11g0266 vs. ExPASy TrEMBL
Match: A0A6J1CYI5 (cytochrome P450 81D1-like OS=Momordica charantia OX=3673 GN=LOC111015737 PE=3 SV=1)

HSP 1 Score: 1060 bits (2740), Expect = 0.0
Identity = 527/529 (99.62%), Postives = 528/529 (99.81%), Query Frame = 0

Query: 1   MHSKILAAMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRT 60
           MHSKILAAMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRT
Sbjct: 1   MHSKILAAMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRT 60

Query: 61  LADISNRHGPVVFLQFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNL 120
           LADISNRHGPVVFL+FGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNL
Sbjct: 61  LADISNRHGPVVFLRFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNL 120

Query: 121 VWSSYGDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSF 180
           VWSSYGDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSF
Sbjct: 121 VWSSYGDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSF 180

Query: 181 FDLTFNVMLRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGF 240
           FDLTFNVMLRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGF
Sbjct: 181 FDLTFNVMLRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGF 240

Query: 241 GWSVEKKMMDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQES 300
           GWSVEKKMMDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQES
Sbjct: 241 GWSVEKKMMDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQES 300

Query: 301 EPDHYTDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLI 360
           EPDHYTDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLI
Sbjct: 301 EPDHYTDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLI 360

Query: 361 DESDMAHLPYLRGIINETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHND 420
           DESDMAHLPYLRGII ETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHND
Sbjct: 361 DESDMAHLPYLRGIIXETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHND 420

Query: 421 PKIWANPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCF 480
           PKIWANPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCF
Sbjct: 421 PKIWANPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCF 480

Query: 481 EWERPGEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLPSQTKTNQAH 529
           EWERPGEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLPSQTKTNQAH
Sbjct: 481 EWERPGEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLPSQTKTNQAH 529

BLAST of MC11g0266 vs. ExPASy TrEMBL
Match: A0A6J1FJC9 (cytochrome P450 81D1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445994 PE=3 SV=1)

HSP 1 Score: 804 bits (2076), Expect = 7.21e-290
Identity = 397/522 (76.05%), Postives = 468/522 (89.66%), Query Frame = 0

Query: 9   MGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADISNRH 68
           MGAI +Y PLF VLY+LTE FLH+IRNLPPTPF SLP+IGHL+LL KP+YRTLA+ISNR+
Sbjct: 1   MGAIIVYIPLFFVLYVLTEQFLHRIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60

Query: 69  GPVVFLQFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSYGDH 128
           GPVVFL+FGSR VLVVSSPS AEECL+KNDVVFA+RPRLLISK FGYNN NLVWSSYGDH
Sbjct: 61  GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120

Query: 129 WRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFDLTFNVM 188
           WR+LRRISTVE+LSTHRLQ+L+ VRL+EV+SLI +LAK ENQ  +  M ++FFDLTFNVM
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAAVRLDEVKSLIGRLAKGENQ--VQDMNNAFFDLTFNVM 180

Query: 189 LRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKKM 248
           LRMI GKRFYG+DV+DADE A  FRELQ++ST+L G +N+GDF+P+M+WLG GW+ EK++
Sbjct: 181 LRMIVGKRFYGEDVEDADE-AHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGWAAEKEI 240

Query: 249 MDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQESEPDHYTDE 308
           M+CH KRDAFMQ LI + + R+    E+E D+SFRDGR+KT+IEVLLELQES+PD Y DE
Sbjct: 241 MECHQKRDAFMQNLINQHKNRAI---ETEPDNSFRDGRKKTLIEVLLELQESDPDQYNDE 300

Query: 309 SIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAHL 368
           +I+ALMLVLL AGTETS+NTMEWALSL+LN+PEVL+KAQ+EID +VG DRL+DESDMA++
Sbjct: 301 TIRALMLVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANV 360

Query: 369 PYLRGIINETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHNDPKIWANPT 428
           P+LRGIINET RMYPPAPLMVPH+SS+DCSVGG+HIPRGTML+VNLWAIHNDPKIWA+P 
Sbjct: 361 PFLRGIINETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPR 420

Query: 429 KFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGEK 488
           KFDPDRF ++ES+K G  F LMPFGSGRRGCPGEGLGLR+IGLVLGSLIQCFEWERPGE+
Sbjct: 421 KFDPDRFVKVESEKLG--FKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEE 480

Query: 489 LVDMTEGIAVSMPKAQPLQAKCRPRPIIANLP-SQTKTNQAH 529
           LVDMTEG+AVSMPKA+ LQAKC+PRPI+  L  SQTK+N A+
Sbjct: 481 LVDMTEGVAVSMPKARHLQAKCKPRPIVDRLHLSQTKSNLAY 514

BLAST of MC11g0266 vs. ExPASy TrEMBL
Match: A0A6J1J228 (cytochrome P450 81D1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481119 PE=3 SV=1)

HSP 1 Score: 791 bits (2044), Expect = 5.35e-285
Identity = 393/522 (75.29%), Postives = 465/522 (89.08%), Query Frame = 0

Query: 9   MGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADISNRH 68
           MGAI LY PLFLVLY+LTE F+HKIRNLPPTPF SLP+IGHL+LL KP+YRTLA+ISNR+
Sbjct: 1   MGAIILYIPLFLVLYVLTEQFIHKIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60

Query: 69  GPVVFLQFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSYGDH 128
           GPVVFL+FGSR VLVVSSPS AEECL+KNDVVFA+RPRLLISK FGYNN NLVWSSYGDH
Sbjct: 61  GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120

Query: 129 WRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFDLTFNVM 188
           WR+LRRISTVE+LSTHRLQ+L+ VRL+EV+SLI +LA+ ENQ  +  M ++FFDLTFNVM
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAGVRLDEVKSLIGRLARGENQ--VQDMNNAFFDLTFNVM 180

Query: 189 LRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKKM 248
           LRMI GKRFYG+DV+DADE A  FRELQ++ST+L G +N+GDF+P+M+WLG G + EK++
Sbjct: 181 LRMIVGKRFYGEDVEDADE-AHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGRAAEKEI 240

Query: 249 MDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQESEPDHYTDE 308
           M+CH KRDAFMQ LI + + R+    E+E  + FRDGR+KT+IEVLLELQES+PD Y DE
Sbjct: 241 MECHQKRDAFMQNLINQHKNRAI---ETEPHNLFRDGRKKTLIEVLLELQESDPDQYNDE 300

Query: 309 SIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAHL 368
           +I+ALMLVLL AGTETS+NTMEWALSL+LN+PEVL+KAQ+EID +VG DRL+DESDMA++
Sbjct: 301 TIRALMLVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANV 360

Query: 369 PYLRGIINETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHNDPKIWANPT 428
           P+LRGIINET RMYPPAPLMVPH+SS+DCSVGG+HIPRGTML+VNLWAIHNDPKIWA+P 
Sbjct: 361 PFLRGIINETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPR 420

Query: 429 KFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGEK 488
           KFDPDRF +++S+K G  F L+PFGSGRRGCPGEGLGLR+IGLVLGSLIQCFEWERPGE 
Sbjct: 421 KFDPDRFVKVDSEKLG--FKLLPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGED 480

Query: 489 LVDMTEGIAVSMPKAQPLQAKCRPRPIIANLP-SQTKTNQAH 529
           LVDMTEG+AVSMPKA+ LQAKC+PRPI+  L  SQTK+N A+
Sbjct: 481 LVDMTEGVAVSMPKARHLQAKCKPRPIVDRLHLSQTKSNLAY 514

BLAST of MC11g0266 vs. ExPASy TrEMBL
Match: A0A1S3BUA3 (LOW QUALITY PROTEIN: cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103493587 PE=3 SV=1)

HSP 1 Score: 791 bits (2042), Expect = 1.16e-284
Identity = 385/521 (73.90%), Postives = 456/521 (87.52%), Query Frame = 0

Query: 8   AMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADISNR 67
           AMGA+FLY PLFLVLY+LT+HFLH IRNLPP PFPSLP++GHL+LLNKPIYR L +ISNR
Sbjct: 4   AMGALFLYIPLFLVLYLLTKHFLHNIRNLPPXPFPSLPILGHLHLLNKPIYRALYNISNR 63

Query: 68  HGPVVFLQFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSYGD 127
           +GPVVFL+ GSR VL+VSS S AEECLTKND++FANRPRLL+SKCFGYNNTNLVWSSYGD
Sbjct: 64  YGPVVFLRLGSRSVLIVSSASAAEECLTKNDIIFANRPRLLLSKCFGYNNTNLVWSSYGD 123

Query: 128 HWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFDLTFNV 187
            WR+LRRI TVE+LSTHRL +LS VR EEVRSLI++L K ENQ  ++ M+H FFDL+FN+
Sbjct: 124 IWRNLRRICTVEILSTHRLHMLSVVRFEEVRSLIQRLLKRENQ--VVNMKHVFFDLSFNL 183

Query: 188 MLRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKK 247
           MLRMI GKRFYGDDVDD +E AK+FRELQA+S+ L+G SN+GDF+P+M+WLGF  ++EK+
Sbjct: 184 MLRMIVGKRFYGDDVDDVEE-AKLFRELQAKSSHLSGKSNMGDFIPLMSWLGFDSTLEKE 243

Query: 248 MMDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQESEPDHYTD 307
           MMDC   RDA MQ+LIE+ R+R   E    IDDSFRDGR+KT+IEVLLELQESEP+ Y D
Sbjct: 244 MMDCQNGRDALMQSLIEQHRRRRIVE----IDDSFRDGRKKTLIEVLLELQESEPEQYND 303

Query: 308 ESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAH 367
           E+I+ LML++L AGTETS + MEWALSLLLNHPE+LKKAQ+EID+QVG  R I+ESDMA 
Sbjct: 304 ETIRGLMLLMLVAGTETSGSIMEWALSLLLNHPEILKKAQIEIDNQVGNQRPIEESDMAR 363

Query: 368 LPYLRGIINETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHNDPKIWANP 427
           LPYLRGIINET RMYPPAPL+VPH+SS+DCSVGG+H+PRGTMLY+N+WAI NDPKIWA+P
Sbjct: 364 LPYLRGIINETLRMYPPAPLLVPHESSQDCSVGGYHVPRGTMLYINIWAIQNDPKIWAHP 423

Query: 428 TKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGE 487
            +FDPDRF+ +ES+KY   FNLMPFG GRRGCPGEGLGLR+IGLVLGSLIQCFEWERP E
Sbjct: 424 KEFDPDRFNDVESEKYK--FNLMPFGLGRRGCPGEGLGLRMIGLVLGSLIQCFEWERPTE 483

Query: 488 KLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLPSQTKTNQA 528
           +LVD+TEGIA++MPKAQ L AKCRPRP+I N+  Q K++ A
Sbjct: 484 ELVDLTEGIAITMPKAQNLHAKCRPRPMIVNILPQAKSSPA 515

BLAST of MC11g0266 vs. ExPASy TrEMBL
Match: A0A0A0KZW4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642310 PE=3 SV=1)

HSP 1 Score: 790 bits (2039), Expect = 3.85e-284
Identity = 384/520 (73.85%), Postives = 456/520 (87.69%), Query Frame = 0

Query: 9   MGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADISNRH 68
           MGAIFLY P FLVLY+LT+HFLH IRNLPPTPFPSLP++GHL+LLNKPIYR L +ISNR+
Sbjct: 9   MGAIFLYIPPFLVLYLLTKHFLHNIRNLPPTPFPSLPILGHLHLLNKPIYRALYNISNRY 68

Query: 69  GPVVFLQFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSYGDH 128
           GPVVFL+ GSR VL+VSSPS AEECLTKND++FANRP LL+SKCFGYNNTNLVWSSYGDH
Sbjct: 69  GPVVFLRLGSRSVLIVSSPSAAEECLTKNDIIFANRPHLLLSKCFGYNNTNLVWSSYGDH 128

Query: 129 WRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFDLTFNVM 188
           WR+LRRI TVE+LSTHRL +L  VR EEVRSLI++L K ENQ  ++ M+H FFDL+FN+M
Sbjct: 129 WRNLRRICTVEILSTHRLHMLYIVRFEEVRSLIQRLVKRENQ--VVNMKHVFFDLSFNLM 188

Query: 189 LRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKKM 248
           LRMI GKRFYGDDVDD +E AK+FRELQAES+RL+G SN+GDF+P+M+WLGF  ++EK+M
Sbjct: 189 LRMIVGKRFYGDDVDDVEE-AKLFRELQAESSRLSGKSNMGDFIPLMSWLGFDSTLEKEM 248

Query: 249 MDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQESEPDHYTDE 308
           +DC   RDA MQ+LIE+ R+R  AE    ID SFRDGR+KT+IEVLLELQESEP+ YTDE
Sbjct: 249 IDCQNGRDALMQSLIEQHRRRRIAE----IDYSFRDGRKKTLIEVLLELQESEPEQYTDE 308

Query: 309 SIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAHL 368
           +I+ALML++L AGTETS + MEWALSLLLNHPE+LKKAQ EID+QVG  RL++ESDMA L
Sbjct: 309 TIRALMLLMLVAGTETSGSIMEWALSLLLNHPEILKKAQTEIDNQVGHKRLMEESDMARL 368

Query: 369 PYLRGIINETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHNDPKIWANPT 428
           PYLRGIINET RMYPPAPL+VPH+SS+DCSVGG+H+PRGTMLY+N+WAI NDPK W +P 
Sbjct: 369 PYLRGIINETLRMYPPAPLLVPHESSQDCSVGGYHVPRGTMLYINIWAIQNDPKFWTHPR 428

Query: 429 KFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGEK 488
           KFDP+RF+ +ES+ Y   FNLM FG GRRGCPGEGLGLR+IGLVLGSLIQCFEWERP E+
Sbjct: 429 KFDPERFNDVESENYK--FNLMAFGLGRRGCPGEGLGLRMIGLVLGSLIQCFEWERPTEE 488

Query: 489 LVDMTEGIAVSMPKAQPLQAKCRPRPIIANLPSQTKTNQA 528
           LVD+TEGIA++MPKAQ L AKCRPRP++ ++ SQTK++ A
Sbjct: 489 LVDLTEGIAITMPKAQNLHAKCRPRPMVVDILSQTKSSPA 519

BLAST of MC11g0266 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 469.5 bits (1207), Expect = 3.4e-132
Identity = 241/493 (48.88%), Postives = 334/493 (67.75%), Query Frame = 0

Query: 35  NLPPTPFPSLPLIGHLYLLNKPIYRTLADISNRHG--PVVFLQFGSRPVLVVSSPSVAEE 94
           NLPP+P  +LP+IGHL LL  P++R    +S   G  P++ L+ G+R V VVSS S+AEE
Sbjct: 29  NLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGDAPIISLRLGNRLVFVVSSHSLAEE 88

Query: 95  CLTKNDVVFANRPRLLISKCFGYNNTNLVWSSYGDHWRSLRRISTVEVLSTHRLQILSTV 154
           C TKNDVV ANR   L SK   Y  T +V +SYGDHWR+LRRI  VE+ S HRL   S++
Sbjct: 89  CFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDHWRNLRRIGAVEIFSAHRLNSFSSI 148

Query: 155 RLEEVRSLIRKLAKSENQD-KILTMRHSFFDLTFNVMLRMIAGKRFYGDDVDDADEDAKI 214
           R +E+  LI  L+++ + +   + M+  F +LTFN ++RM+AGK +YGD  +D D +AK 
Sbjct: 149 RRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLTFNNIIRMLAGKCYYGDGAED-DPEAKR 208

Query: 215 FRELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKKMMDCHYKRDAFMQTLIEEQRKRST 274
            REL AE     G  N  D++P++TW+      EK++     + D F+Q L++E+R+   
Sbjct: 209 VRELIAEGMGCFGAGNTADYLPILTWI---TGSEKRIKKIASRLDEFLQGLVDERREG-- 268

Query: 275 AETESEIDDSFRDGRRKTMIEVLLELQESEPDHYTDESIKALMLVLLTAGTETSVNTMEW 334
                      ++ R+ TM++ LL LQE++P++YTD  IK +ML L+ AGT+TS  T+EW
Sbjct: 269 -----------KEKRQNTMVDHLLCLQETQPEYYTDNIIKGIMLSLILAGTDTSAVTLEW 328

Query: 335 ALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAHLPYLRGIINETFRMYPPAPLMVPH 394
            LS LLNHP++L KA+ EID++VG +RL++ESD++HLPYL+ I++E+ R+YP +PL+VPH
Sbjct: 329 TLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQNIVSESLRLYPASPLLVPH 388

Query: 395 QSSEDCSVGGHHIPRGTMLYVNLWAIHNDPKIWANPTKFDPDRFDRLESDKYGYGFNLMP 454
            +SEDC VGG+H+PRGTML  N WAIH DPKIW +PT F P+RF     +K G    L+ 
Sbjct: 389 VASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPERF-----EKEGEAQKLLG 448

Query: 455 FGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGEKLVDMTE-GIAVSMPKAQPLQAKC 514
           FG GRR CPG GL  R+  L +GSLIQCFEWER GE+ VDMTE G  V MPKA PL A C
Sbjct: 449 FGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEGGGGVIMPKAIPLVAMC 499

Query: 515 RPRPIIANLPSQT 524
           + RP++  + +++
Sbjct: 509 KARPVVGKILNES 499

BLAST of MC11g0266 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 463.8 bits (1192), Expect = 1.9e-130
Identity = 241/508 (47.44%), Postives = 344/508 (67.72%), Query Frame = 0

Query: 18  LFLVLYILTEHFLHKIR---NLPPTPFPSLPLIGHLYLLNKPIYRTLADISN--RHGPVV 77
           LF+VL ++  + + K++   NLPP+P  SLP+IGHL LL  PI+RT   +S    + P+ 
Sbjct: 11  LFVVLSLI--YLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAPIF 70

Query: 78  FLQFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSYGDHWRSL 137
            L+ G+R V V SS S+AEEC TKNDVV ANRP  +++K   Y+ T ++ +SYGDHWR+L
Sbjct: 71  SLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRNL 130

Query: 138 RRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKI-LTMRHSFFDLTFNVMLRM 197
           RRI +VE+ S HRL    ++R +E+R L+ +L+++ +Q+ + + M+    DLTFN +LRM
Sbjct: 131 RRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNILRM 190

Query: 198 IAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKKMMDC 257
           +AGKR+YGD V+D D +AK  R+L A+     G  N  D++P++  +      E ++   
Sbjct: 191 VAGKRYYGDGVED-DPEAKRVRQLIADVVACAGAGNAVDYLPVLRLVS---DYETRVKKL 250

Query: 258 HYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQESEPDHYTDESIK 317
             + D F+Q L++E+R+                 +  TMI+ LL LQES+PD++TD  IK
Sbjct: 251 AGRLDEFLQGLVDEKREAKE--------------KGNTMIDHLLTLQESQPDYFTDRIIK 310

Query: 318 ALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAHLPYL 377
             ML L+ AGT+TS  T+EWALS +LNHP+VL KA+ EID ++G DRL+DESD+++LPYL
Sbjct: 311 GNMLALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYL 370

Query: 378 RGIINETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHNDPKIWANPTKFD 437
           + I++ET R+YP AP+++PH +SEDC V G+ +PRGT+L  N+WAIH DP++W +P  F 
Sbjct: 371 QNIVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFK 430

Query: 438 PDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGEKLVD 497
           P+RF     +K G    LMPFG GRR CPG GL  R+I L LGSLIQC EWE+ GE+ VD
Sbjct: 431 PERF-----EKEGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VD 490

Query: 498 MTEGIAVSMPKAQPLQAKCRPRPIIANL 520
           M+EG  V+MPKA+PL+A CR RP +  +
Sbjct: 491 MSEGKGVTMPKAKPLEAMCRARPSVVKI 492

BLAST of MC11g0266 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 457.6 bits (1176), Expect = 1.4e-128
Identity = 237/517 (45.84%), Postives = 340/517 (65.76%), Query Frame = 0

Query: 6   LAAMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADIS 65
           + A+  IF +  + L L  L      K+ NLPP+P  +LP+IGHL LL  P++R    +S
Sbjct: 1   MEALMLIFTFCFIVLSLIFLIGRIKRKL-NLPPSPAWALPVIGHLRLLKPPLHRVFLSVS 60

Query: 66  NRHG--PVVFLQFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWS 125
              G  P++ L+ G+R + VVSS S+AEEC TKNDV+ ANR   + +K   Y N+ +V +
Sbjct: 61  QSLGDAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSA 120

Query: 126 SYGDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQD-KILTMRHSFFD 185
           SY +HWR+LRRI  +E+ S HRL   S++R +E+R LI +L ++ +     + M+  F D
Sbjct: 121 SYSEHWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSD 180

Query: 186 LTFNVMLRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGW 245
           LTFN ++RM+AGK +YGD  +D D +AK  R L AE+   +G  N  D++P++TW+ +  
Sbjct: 181 LTFNNIIRMLAGKCYYGDGKED-DPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYSE 240

Query: 246 SVEKKMMDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQESEP 305
           +  KK+     + D F+Q L++E+R+              ++ +  TM++ LL LQE++P
Sbjct: 241 TRIKKLAG---RLDEFLQGLVDEKREG-------------KEKKENTMVDHLLCLQETQP 300

Query: 306 DHYTDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDE 365
           ++Y D  IK  ML L+  GT+T+  T+EWALS LLN+PEVL KA+ EID  +G DRL++E
Sbjct: 301 EYYMDRIIKGTMLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEE 360

Query: 366 SDMAHLPYLRGIINETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHNDPK 425
           SD+ +LPYL+ I++ET R+YP AP+++PH +S+DC VGG+ +PRGTML  N WAIH DP 
Sbjct: 361 SDIPNLPYLQNIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPL 420

Query: 426 IWANPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEW 485
           +W +PT F P+RF     +K G    LMPFG GRR CPG GL  R++ L LGSLIQCFEW
Sbjct: 421 LWDDPTSFKPERF-----EKEGEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEW 480

Query: 486 ERPGEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANL 520
           ER GE+ VDMTEG  ++MPKA+PL+A CR R  +  +
Sbjct: 481 ERIGEEEVDMTEGPGLTMPKARPLEAMCRARDFVGKI 494

BLAST of MC11g0266 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 456.4 bits (1173), Expect = 3.0e-128
Identity = 242/511 (47.36%), Postives = 334/511 (65.36%), Query Frame = 0

Query: 12  IFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLADISNRHG-- 71
           I  +  LF +   L      K+ NLPP+P  SLP+IGHL+LL  P++RT   +S   G  
Sbjct: 8   ILSFLSLFTLAIFLLTRSKRKL-NLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSIGNA 67

Query: 72  PVVFLQFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSYGDHW 131
           PV  L+ G+R V V+SS S+AEEC TKNDVV ANRP+  ISK  GYN T L+ +SYGDHW
Sbjct: 68  PVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDHW 127

Query: 132 RSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKI-LTMRHSFFDLTFNVM 191
           R+LRRI+ VE+ STHRL     +R +E+R LI  L++      + + M+    +L  N  
Sbjct: 128 RNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLTNLASNTT 187

Query: 192 LRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKKM 251
           +RM+AGKR++G+D    ++DAK+ + L +E+    G  N  D++ ++ W+    S EK++
Sbjct: 188 IRMLAGKRYFGED----NDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWVS---SYEKRI 247

Query: 252 MDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQESEPDHYTDE 311
            +   + D F+Q L++E+R    AE E          + +TMI+ LL LQ+ +PD+YTD 
Sbjct: 248 KNLGNRFDTFLQKLVDEKR----AEKE----------KGETMIDHLLALQDIQPDYYTDV 307

Query: 312 SIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAHL 371
            IK ++L L+ AGT+TS  T+EWA+S LLNHPE+LKKA++EID++VG DRL+DESD+ +L
Sbjct: 308 IIKGIILTLIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNL 367

Query: 372 PYLRGIINETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHNDPKIWANPT 431
            YL+ I+ ET RMYP  PL++PH SSEDC VGG+ IP GTM+  N WA+H DP++W +P 
Sbjct: 368 SYLQSIVLETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPE 427

Query: 432 KFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGEK 491
            F P+RF     +K G    L+ FG GRR CPG GL  R+I   LGSL+QCFEWER GE 
Sbjct: 428 IFKPERF-----EKEGEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGED 487

Query: 492 LVDMTEGIAVSMPKAQPLQAKCRPRPIIANL 520
            VDMTE    ++PKA PL+A C+ R I+  L
Sbjct: 488 FVDMTEDKGATLPKAIPLRAMCKARSIVDKL 491

BLAST of MC11g0266 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 454.5 bits (1168), Expect = 1.1e-127
Identity = 235/504 (46.63%), Postives = 335/504 (66.47%), Query Frame = 0

Query: 19  FLVLYILTEHFLHKIR---NLPPTPFPSLPLIGHLYLLNKPIYRTLADISNRHG--PVVF 78
           FL L I  +  L K     NLPP+P   LP+IGHL+LL +P++RT   IS   G  P+  
Sbjct: 11  FLFLTISIKLLLTKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSLGNAPIFH 70

Query: 79  LQFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNLVWSSYGDHWRSLR 138
           L+ G+R V V+SS S+AEEC TKNDVV ANRP ++++K  GYN TN++ +SYGDHWR+LR
Sbjct: 71  LRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYGDHWRNLR 130

Query: 139 RISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKI-LTMRHSFFDLTFNVMLRMI 198
           RI+ VE+ S+HR+   S++R +E+R LI  L++      + + ++    +L FN ++ M+
Sbjct: 131 RIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNLAFNNIIMMV 190

Query: 199 AGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGWSVEKKMMDCH 258
           AGKR+YG   +D DE AK+ REL AE     G  N+ D++P + W+    + E +     
Sbjct: 191 AGKRYYGTGTEDNDE-AKLVRELIAEIMAGAGSGNLADYLPSINWV---TNFENQTKILG 250

Query: 259 YKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQESEPDHYTDESIKA 318
            + D  +Q L++E+R    AE E          + +T+I+ LL  QE+EP++YTD  IK 
Sbjct: 251 NRLDRVLQKLVDEKR----AEKE----------KGQTLIDHLLSFQETEPEYYTDVIIKG 310

Query: 319 LMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDESDMAHLPYLR 378
           ++L L+ AGT+TS  T+EWA+S LLNHPE+L+KA+ EIDD++G DRL++ESD+ +L YL+
Sbjct: 311 IILALVLAGTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQ 370

Query: 379 GIINETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHNDPKIWANPTKFDP 438
            I++ET R+YP  PL++PH SS++C V G+ +PR T+L  N+WA+H DP +W  P +F P
Sbjct: 371 NIVSETLRLYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKP 430

Query: 439 DRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEWERPGEKLVDM 498
           +RF     +K G    LMPFG GRR CPG  LG R++ L LG LIQ FEWER G +LVDM
Sbjct: 431 ERF-----EKEGEARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDM 490

Query: 499 TEGIAVSMPKAQPLQAKCRPRPII 517
           TEG  ++MPKA PL+A C+ R I+
Sbjct: 491 TEGEGITMPKATPLRAMCKARAIV 491

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
W8JMU76.3e-13950.51Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1[more]
Q9LHA12.1e-12646.71Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1[more]
Q9FG654.7e-12647.10Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1[more]
Q0WTF44.7e-12645.63Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1[more]
Q6WNQ81.0e-12546.63Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022146589.10.099.43cytochrome P450 81D1-like [Momordica charantia][more]
XP_038896683.15.59e-29477.26cytochrome P450 81Q32-like [Benincasa hispida][more]
XP_022940352.11.49e-28976.05cytochrome P450 81D1-like isoform X1 [Cucurbita moschata][more]
XP_023523281.11.65e-28675.29cytochrome P450 81D1-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022982225.11.10e-28475.29cytochrome P450 81D1-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1CYI50.099.62cytochrome P450 81D1-like OS=Momordica charantia OX=3673 GN=LOC111015737 PE=3 SV... [more]
A0A6J1FJC97.21e-29076.05cytochrome P450 81D1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1114459... [more]
A0A6J1J2285.35e-28575.29cytochrome P450 81D1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481119... [more]
A0A1S3BUA31.16e-28473.90LOW QUALITY PROTEIN: cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A0A0KZW43.85e-28473.85Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642310 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G37340.13.4e-13248.88cytochrome P450, family 81, subfamily D, polypeptide 3 [more]
AT4G37370.11.9e-13047.44cytochrome P450, family 81, subfamily D, polypeptide 8 [more]
AT4G37360.11.4e-12845.84cytochrome P450, family 81, subfamily D, polypeptide 2 [more]
AT4G37330.13.0e-12847.36cytochrome P450, family 81, subfamily D, polypeptide 4 [more]
AT4G37320.11.1e-12746.63cytochrome P450, family 81, subfamily D, polypeptide 5 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 459..470
score: 31.47
coord: 320..337
score: 37.92
coord: 450..459
score: 57.38
coord: 373..384
score: 36.81
IPR001128Cytochrome P450PFAMPF00067p450coord: 38..502
e-value: 1.1E-95
score: 321.1
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 372..390
score: 32.21
coord: 89..110
score: 27.24
coord: 459..482
score: 36.29
coord: 65..84
score: 35.95
coord: 309..326
score: 31.91
coord: 183..201
score: 29.34
coord: 329..355
score: 40.82
coord: 413..437
score: 39.02
coord: 449..459
score: 55.41
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 30..517
e-value: 1.8E-124
score: 417.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 37..513
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 19..483
e-value: 1.9E-19
score: 67.7
NoneNo IPR availablePANTHERPTHR47947:SF24CYTOCHROME P450 81E8coord: 19..522
NoneNo IPR availablePANTHERPTHR47947CYTOCHROME P450 82C3-RELATEDcoord: 19..522
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 452..461

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC11g0266.1MC11g0266.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098542 defense response to other organism
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004190 aspartic-type endopeptidase activity
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen