MC10g_new0352 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g_new0352
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Methionine gamma-lyase
Location	MC10: 17376489 .. 17379633 (+)
RNA-Seq Expression	MC10g_new0352
Synteny	MC10g_new0352

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTAACTATCCATAACAATGAACATTAATAGAAGATTCTAGGGCACTTGAAATCTAACCGCTGTGATGACCTTGCTCCAATGCTTCTCCTCATCGAATGAGTCTCTTTGGATTTGTTTGGATGAATCAGTTCGATCAGTTTGACTCTTGATCCACGTCCTCATTTCATGTATAAATCTAATACAGTAATTTCACCTTGTTCGTTTCTCGTTCAAAACCATTGGTTGAAACAAAAAAAAATATGTTACAATTCAATAAAACTGAAACATCATGGGAGGTCCTCACCAAATATAAACATCATTCCAAACCAACTTCAATTTGGCATCTCTAAACAAACTCAAACTCATCCGTCCATCCACTTTCGTGTTTAATTTTACGTTTTACAAAAAAAAAAACCCCCCACATTTAATTTTCCACAAATCCCATTTGGAAACCAAATCCTGACCACATTCACTGTACACCCAATTTCATCCTCTCCCCTATATAAAGCAAACCCAATAATCCCAAAAACTACACGAAGCTATAAATAAAATTAAAATTTTTGAAATTCTCTGATATCTATTTTTCCCATGGCAGAAATCCGAGCCCTTAATTCCCCCTTGAAATTCTCCGGCGCCAAAAGACCCGGCCCCGACGACACCAACGGCGGCGGAAACGACGAGTCGTATTTCATGTCGAAGAAATCGGCGGCGGGCATGGCGGTGGAGGACGATCCGGCGGCTGCGATCGCGAACGCGCGGCACGAGTTCGGAGAACACGGAGGGGTAAACATGTCAATTGAGGCGTCCGCCACGTTCACGGTGATGGAGCCGGAGACGATGCGGCGGATGTTCTCCGGCGAGCTGGGGCCGGACCGGGACTTCTTCATCTACAGCCGTCACTTCAACCCGACGGTGCTGAATCTGAGCCGTCAGATGGCGGCCATGGAGGGCACCGAGGCCGCGTACTGCACCTCGAGCGGAATGTCCGCGATCGCTGCGGTGCTGCTGCAACTGCTCGCGAGTGGGGACCACATCGTGGCCTCGCGGACGCTGTATGGCGGGACCCACGCGCTGCTGAGTCATTTCTTGCCACGTACGTCGGGGATAACGACGTCGTTTGTGGACGTGGGGAATGTGGAGATGGTGAGGGCGGGGATTGTGGAGGGAAAGACGAAGGTTCTGTATTTCGAGGCGGTGTCGAATCCGACGCTGGCGGTGGCGGACGTGCCGGAGCTCTGCCGGATCGGACACGACCACGGCCTCACGGTGGTCGTCGACAACACTTTCGCGCCGATGGTGCTGTCGCCGGCCAGGCTCGGCGCCGACGTCGTCATTCACAGTATCTCCAAGTTTATTAGCGGTGGAGCTGACGTCATCGCAGGTACTCTTATTAACTCTACTGTGTTCTGTTCTTGTTTTAAAAAGCCGAAAATCAAATTATTGGTCAAATTTCAAAATTGAAAAATGTGTTTTTAATTTGTTTTTTTTAATGTATTTTATAAGTTTTATTAAGAAAAAAAAAAACTAAAAATCAAATGATTAAATATATGGATGAAAGTAGATACTAGACAAGAAGATAACAATAAAAATTAATTGGGACTAAAGTAGATATTTTGACTGTTTAAAAATTAAAAAATATATTTTATTTTATTTTAGTTGAGAGATTAAAATATATTTTTTCTATTTAGGTGTAAGTTTACAACAAACATAAAATATATGACATACTAAGATAATTTTTAATTTCTGTCCTTAATTTTTTAAAGATCTTTAATTTTATCCTAGTTAAGTTGAATGATGGTGATTATATGATATAGTTCAATTACCGTTGAGCTGTGCTCTTTTTGATTATGAATATGCTACTTAAATTATTAAAAAAGTGGAATATACTACCCATGACACATCAAATCCAATTTTTTTAACTCACCAATTAATAAAATCTATTTCTTTCCATTTATTTTCCAATTTATAATAATGACTTAACTTTTTTTTAATTTAATATATTAAAACTAAAATTGAGAATTTTAAAATATTAATAGTTTAGTACACAACTTTTGCAGCCTAACTTTGAAACATTGCCCTACCATTGGTATCTCCATCCCTATCCCAACGAAAGAACTTTTAGAAAAAGTGGGACACGTTTGAAAGACCACATGAATAATACGACAATGAGTCATAGGTCACGAGGCACCAACATCAACCACACACACACAACACATCATCACTCATCAGTACATCACACATATCCCCATCAATGTTTGTGAATCTTCGTTTACTTTTCCATTAGAGAAAACAAATGCCATATGGATGCGAGAAAAAGATTAAATATATCGTCGCTTATTAGCTTAAGCGCTTTTAAATCTTGTAGTGATTTAACATGGAATGACTTGGTGTGTAGGCGCTGTGTGCGGACCTGCAAAGTTGATAAACTCAATGATGGACTTGCGACAGGGGAGCCTAATGCTTTTGGGGCCAACAATGAATGCAAAGGTGGCGTTCGAGCTCTCGGAGAGGATTCCGCACTTGAGCTTGAGAATGAAGGAGCATTGTAGAAGAGCTGCAGAGTTTGCAGAGCGGATGAAGAAGGCAGGGTTGAAGGTCATATACCCAGGGCTACCAGACCACCCACAACACAAGTTAATGAAGAGGCTTGCAAACTCGGAGTATGGGTTCGGGGGGATGTTGTGCTTAGACATGGGAACTGAGGAAAGAGCCAACAGGCTCATGAGCGTGCTCCAAAACAACACCCAATTCGGGTTCATGGCAGTGAGTTTAGGGTACTATGAGACCCTTATGTCGTGCTCGGGTAGCAGCACGAGTAGTGAGATGAACAGGGAGGAGAAGCAACTGGCTGGGATCTCACCCGGACTCATAAGAATGTCCATTGGATATATGGGTACGTTCGAGCAAAAATGGAGCCAATTTGAGAAGGCTCTTGCTAAAGTGCAAGATATGGGCCTTCCATTTTGCAACAATAACTAATTTTAGTACAACAAATGTGAGGTGTGAGAATTTGAACATTCAACTTTAAAATGAGTTATATATATTTACTGCTAAACTAGGTTTATATTAGCAGTAAGCCTCCTCTTGTATCCAGAGTGCCTTCTGCTTCCTTTCTCCAGTTTCATTAGTTTAGTACTTTTGGACCGTGGACTTAGTTTCCTTTGGC

mRNA sequence

ATGGCAGAAATCCGAGCCCTTAATTCCCCCTTGAAATTCTCCGGCGCCAAAAGACCCGGCCCCGACGACACCAACGGCGGCGGAAACGACGAGTCGTATTTCATGTCGAAGAAATCGGCGGCGGGCATGGCGGTGGAGGACGATCCGGCGGCTGCGATCGCGAACGCGCGGCACGAGTTCGGAGAACACGGAGGGGTAAACATGTCAATTGAGGCGTCCGCCACGTTCACGGTGATGGAGCCGGAGACGATGCGGCGGATGTTCTCCGGCGAGCTGGGGCCGGACCGGGACTTCTTCATCTACAGCCGTCACTTCAACCCGACGGTGCTGAATCTGAGCCGTCAGATGGCGGCCATGGAGGGCACCGAGGCCGCGTACTGCACCTCGAGCGGAATGTCCGCGATCGCTGCGGTGCTGCTGCAACTGCTCGCGAGTGGGGACCACATCGTGGCCTCGCGGACGCTGTATGGCGGGACCCACGCGCTGCTGAGTCATTTCTTGCCACGTACGTCGGGGATAACGACGTCGTTTGTGGACGTGGGGAATGTGGAGATGGTGAGGGCGGGGATTGTGGAGGGAAAGACGAAGGTTCTGTATTTCGAGGCGGTGTCGAATCCGACGCTGGCGGTGGCGGACGTGCCGGAGCTCTGCCGGATCGGACACGACCACGGCCTCACGGTGGTCGTCGACAACACTTTCGCGCCGATGGTGCTGTCGCCGGCCAGGCTCGGCGCCGACGTCGTCATTCACAGTATCTCCAAGTTTATTAGCGGTGGAGCTGACGTCATCGCAGGCGCTGTGTGCGGACCTGCAAAGTTGATAAACTCAATGATGGACTTGCGACAGGGGAGCCTAATGCTTTTGGGGCCAACAATGAATGCAAAGGTGGCGTTCGAGCTCTCGGAGAGGATTCCGCACTTGAGCTTGAGAATGAAGGAGCATTGTAGAAGAGCTGCAGAGTTTGCAGAGCGGATGAAGAAGGCAGGGTTGAAGGTCATATACCCAGGGCTACCAGACCACCCACAACACAAGTTAATGAAGAGGCTTGCAAACTCGGAGTATGGGTTCGGGGGGATGTTGTGCTTAGACATGGGAACTGAGGAAAGAGCCAACAGGCTCATGAGCGTGCTCCAAAACAACACCCAATTCGGGTTCATGGCAGTGAGTTTAGGGTACTATGAGACCCTTATGTCGTGCTCGGGTAGCAGCACGAGTAGTGAGATGAACAGGGAGGAGAAGCAACTGGCTGGGATCTCACCCGGACTCATAAGAATGTCCATTGGATATATGGGTACGTTCGAGCAAAAATGGAGCCAATTTGAGAAGGCTCTTGCTAAAGTGCAAGATATGGGCCTTCCATTTTGCAACAATAACTAA

Coding sequence (CDS)

ATGGCAGAAATCCGAGCCCTTAATTCCCCCTTGAAATTCTCCGGCGCCAAAAGACCCGGCCCCGACGACACCAACGGCGGCGGAAACGACGAGTCGTATTTCATGTCGAAGAAATCGGCGGCGGGCATGGCGGTGGAGGACGATCCGGCGGCTGCGATCGCGAACGCGCGGCACGAGTTCGGAGAACACGGAGGGGTAAACATGTCAATTGAGGCGTCCGCCACGTTCACGGTGATGGAGCCGGAGACGATGCGGCGGATGTTCTCCGGCGAGCTGGGGCCGGACCGGGACTTCTTCATCTACAGCCGTCACTTCAACCCGACGGTGCTGAATCTGAGCCGTCAGATGGCGGCCATGGAGGGCACCGAGGCCGCGTACTGCACCTCGAGCGGAATGTCCGCGATCGCTGCGGTGCTGCTGCAACTGCTCGCGAGTGGGGACCACATCGTGGCCTCGCGGACGCTGTATGGCGGGACCCACGCGCTGCTGAGTCATTTCTTGCCACGTACGTCGGGGATAACGACGTCGTTTGTGGACGTGGGGAATGTGGAGATGGTGAGGGCGGGGATTGTGGAGGGAAAGACGAAGGTTCTGTATTTCGAGGCGGTGTCGAATCCGACGCTGGCGGTGGCGGACGTGCCGGAGCTCTGCCGGATCGGACACGACCACGGCCTCACGGTGGTCGTCGACAACACTTTCGCGCCGATGGTGCTGTCGCCGGCCAGGCTCGGCGCCGACGTCGTCATTCACAGTATCTCCAAGTTTATTAGCGGTGGAGCTGACGTCATCGCAGGCGCTGTGTGCGGACCTGCAAAGTTGATAAACTCAATGATGGACTTGCGACAGGGGAGCCTAATGCTTTTGGGGCCAACAATGAATGCAAAGGTGGCGTTCGAGCTCTCGGAGAGGATTCCGCACTTGAGCTTGAGAATGAAGGAGCATTGTAGAAGAGCTGCAGAGTTTGCAGAGCGGATGAAGAAGGCAGGGTTGAAGGTCATATACCCAGGGCTACCAGACCACCCACAACACAAGTTAATGAAGAGGCTTGCAAACTCGGAGTATGGGTTCGGGGGGATGTTGTGCTTAGACATGGGAACTGAGGAAAGAGCCAACAGGCTCATGAGCGTGCTCCAAAACAACACCCAATTCGGGTTCATGGCAGTGAGTTTAGGGTACTATGAGACCCTTATGTCGTGCTCGGGTAGCAGCACGAGTAGTGAGATGAACAGGGAGGAGAAGCAACTGGCTGGGATCTCACCCGGACTCATAAGAATGTCCATTGGATATATGGGTACGTTCGAGCAAAAATGGAGCCAATTTGAGAAGGCTCTTGCTAAAGTGCAAGATATGGGCCTTCCATTTTGCAACAATAACTAA

Protein sequence

MAEIRALNSPLKFSGAKRPGPDDTNGGGNDESYFMSKKSAAGMAVEDDPAAAIANARHEFGEHGGVNMSIEASATFTVMEPETMRRMFSGELGPDRDFFIYSRHFNPTVLNLSRQMAAMEGTEAAYCTSSGMSAIAAVLLQLLASGDHIVASRTLYGGTHALLSHFLPRTSGITTSFVDVGNVEMVRAGIVEGKTKVLYFEAVSNPTLAVADVPELCRIGHDHGLTVVVDNTFAPMVLSPARLGADVVIHSISKFISGGADVIAGAVCGPAKLINSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLSLRMKEHCRRAAEFAERMKKAGLKVIYPGLPDHPQHKLMKRLANSEYGFGGMLCLDMGTEERANRLMSVLQNNTQFGFMAVSLGYYETLMSCSGSSTSSEMNREEKQLAGISPGLIRMSIGYMGTFEQKWSQFEKALAKVQDMGLPFCNNN

Homology

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BLAST of MC10g_new0352 vs. ExPASy Swiss-Prot
Match: Q9SGU9 (Methionine gamma-lyase OS=Arabidopsis thaliana OX=3702 GN=MGL PE=1 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 1.2e-183
Identity = 321/447 (71.81%), Postives = 374/447 (83.67%), Query Frame = 0


Query: 1   MAEIRALNSPLKFSGAKRPGPDDTNGGGNDESYFMSKKSAAGMAVEDDPAAAIANARHEF 60
           MA       PL FSG KR   DD +G        ++KKSA  +  + DPAAAIAN RHEF
Sbjct: 1   MAHFLETQEPLVFSGKKRNDRDDEDGDA-----LVAKKSALAVC-DADPAAAIANIRHEF 60

Query: 61  GEHGGVNMSIEASATFTVMEPETMRRMFSGELGPDRDFFIYSRHFNPTVLNLSRQMAAME 120
           GEHGGVNMSIEASATFTVMEP+TMRRMF+GELGPD DF++YSRHFNPTVLNLSRQMAA+E
Sbjct: 61  GEHGGVNMSIEASATFTVMEPDTMRRMFTGELGPDNDFYVYSRHFNPTVLNLSRQMAALE 120

Query: 121 GTEAAYCTSSGMSAIAAVLLQLLASGDHIVASRTLYGGTHALLSHFLPRTSGITTSFVDV 180
           GT+AAYCTSSGMSAI++V+LQL +SG H+VA+ TLYGGTHALLSHFLPRT  ITTSFVD+
Sbjct: 121 GTQAAYCTSSGMSAISSVMLQLCSSGGHVVAASTLYGGTHALLSHFLPRTCNITTSFVDI 180

Query: 181 GNVEMVRAGIVEGKTKVLYFEAVSNPTLAVADVPELCRIGHDHGLTVVVDNTFAPMVLSP 240
            +   V   IVEG+T+VLYFE+V+NPTL VAD+PEL R+ H+ G+TVVVDNTFAPMVLSP
Sbjct: 181 TDHGAVANAIVEGRTQVLYFESVANPTLTVADIPELSRMAHEKGVTVVVDNTFAPMVLSP 240

Query: 241 ARLGADVVIHSISKFISGGADVIAGAVCGPAKLINSMMDLRQGSLMLLGPTMNAKVAFEL 300
           A+LGADVV+HSISKFISGGAD+IAGAVCG   L+  MMDLR GSLMLLGPTMNAKVAFEL
Sbjct: 241 AKLGADVVVHSISKFISGGADIIAGAVCGSENLVKEMMDLRGGSLMLLGPTMNAKVAFEL 300

Query: 301 SERIPHLSLRMKEHCRRAAEFAERMKKAGLKVIYPGLPDHPQHKLMKRLANSEYGFGGML 360
           SERIPHL LRM+EH  RA  +AERM+  G+KVIYPGL  HPQHKL K + N +YG+GG+L
Sbjct: 301 SERIPHLGLRMREHSHRAQVYAERMRDLGMKVIYPGLETHPQHKLFKGMVNRDYGYGGLL 360

Query: 361 CLDMGTEERANRLMSVLQNNTQFGFMAVSLGYYETLMSCSGSSTSSEMNREEKQLAGISP 420
            +DM TEE+AN+LM+ LQN TQFGFMAVSLGYYETLMSCSGSSTSSE++  +K+ AGISP
Sbjct: 361 SIDMETEEKANKLMAYLQNATQFGFMAVSLGYYETLMSCSGSSTSSELDPSQKEAAGISP 420

Query: 421 GLIRMSIGYMGTFEQKWSQFEKALAKV 448
           GL+RMS+GY+GT EQKW+QFEKA  ++
Sbjct: 421 GLVRMSVGYVGTLEQKWTQFEKAFLRM 441

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BLAST of MC10g_new0352 vs. ExPASy Swiss-Prot
Match: Q8L0X4 (L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. polymorphum OX=76857 GN=mgl PE=1 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 5.4e-54
Identity = 140/389 (35.99%), Postives = 217/389 (55.78%), Query Frame = 0


Query: 63  HGGVNMSIEASATFTVMEPETMRRMFSGELGPDRDFFIYSRHFNPTVLNLSRQMAAMEGT 122
           +G + M I  ++TF     E   R F+ E       +IY+R  NPT   L  ++AA+E  
Sbjct: 22  YGTLAMPIYQTSTFIFDSAEQGGRRFALE----EAGYIYTRLGNPTTTVLENKIAALEEG 81

Query: 123 EAAYCTSSGMSAIAAVLLQLLASGDHIVASRTLYGGTHALLSHFLPRTSGITTSFVDVGN 182
           EAA  TSSGM AI++ L  +L +GDH+V  +TLYG T AL+ H L R  GI  +FVD  N
Sbjct: 82  EAAVATSSGMGAISSTLWTVLKAGDHVVTDKTLYGCTFALMCHGLTR-FGIEVTFVDTSN 141

Query: 183 VEMVRAGIVEGKTKVLYFEAVSNPTLAVADVPELCRIGHDHGLT-VVVDNTFA-PMVLSP 242
           ++ V+   ++  T+V+Y E  +NP L + D+  L ++ H +  T V+VDNTFA P +  P
Sbjct: 142 LDEVK-NAMKKNTRVVYLETPANPNLKIVDLEALSKLAHTNPNTLVIVDNTFATPYMQKP 201

Query: 243 ARLGADVVIHSISKFISGGADVIAGAVCGPAKLINSMMDLRQGSLMLLGPTMNAKVAFEL 302
            +LGAD+V+HS++K+I+G  DVIAG V    +L + +  +  G   + G  +  + A+ +
Sbjct: 202 LKLGADIVVHSVTKYINGHGDVIAGLVITNKELADQIRFI--GLKDMTGAVLGPQDAYYI 261

Query: 303 SERIPHLSLRMKEHCRRAAEFAERMKKAGL--KVIYPGLPDHPQHKLMKRLANSEYGFGG 362
              +    +RM+ HC+ A +  E + K     +V YPGL  HP H++ K+       FG 
Sbjct: 262 IRGMKTFEIRMERHCKNAKKVVEFLNKHPKIERVYYPGLETHPGHEIAKKQMKD---FGA 321

Query: 363 MLCLDMGTEERANRLMSVLQNNTQFGFMAVSLGYYETLMSCSGSSTSSEMNREEKQLAGI 422
           M+  ++     A +    L NN +   +AVSLG  ETL+    S T S   +EE++ AGI
Sbjct: 322 MISFELKGGFEAGK---TLLNNLKLCSLAVSLGDTETLIQHPASMTHSPYTKEEREAAGI 381

Query: 423 SPGLIRMSIGYMGTFEQKWSQFEKALAKV 448
           + GL+R+S+G +   E   +  E+ L K+
Sbjct: 382 TDGLVRLSVG-LENVEDIIADLEQGLEKI 395

[Close]

BLAST of MC10g_new0352 vs. ExPASy Swiss-Prot
Match: Q8RDT4 (L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / DSM 15643 / BCRC 10681 / CIP 101130 / JCM 8532 / KCTC 2640 / LMG 13131 / VPI 4355) OX=190304 GN=FN1419 PE=1 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 6.6e-52
Identity = 136/389 (34.96%), Postives = 214/389 (55.01%), Query Frame = 0


Query: 63  HGGVNMSIEASATFTVMEPETMRRMFSGELGPDRDFFIYSRHFNPTVLNLSRQMAAMEGT 122
           +G + M I  ++TF     E   R F+ E       +IY+R  NPT   L  ++AA+E  
Sbjct: 22  YGTLAMPIYQTSTFIFDSAEQGGRRFALE----EAGYIYTRLGNPTTTVLENKIAALEEG 81

Query: 123 EAAYCTSSGMSAIAAVLLQLLASGDHIVASRTLYGGTHALLSHFLPRTSGITTSFVDVGN 182
           EA    SSGM AI++ L  +L +GDH+V  +TLYG T AL++H L R  G+  +FVD  N
Sbjct: 82  EAGIAMSSGMGAISSTLWTVLKAGDHVVTDKTLYGCTFALMNHGLTR-FGVEVTFVDTSN 141

Query: 183 VEMVRAGIVEGKTKVLYFEAVSNPTLAVADVPELCRIGHDHGLT-VVVDNTFA-PMVLSP 242
           +E V+   ++  T+V+Y E  +NP L + D+  L +I H +  T V+VDNTFA P +  P
Sbjct: 142 LEEVK-NAMKKNTRVVYLETPANPNLKIVDLEALSKIAHTNPNTLVIVDNTFATPYMQKP 201

Query: 243 ARLGADVVIHSISKFISGGADVIAGAVCGPAKLINSMMDLRQGSLMLLGPTMNAKVAFEL 302
            +LG D+V+HS +K+++G  DVIAG V    +L + +  +  G   + G  +  + A+ +
Sbjct: 202 LKLGVDIVVHSATKYLNGHGDVIAGLVVTRQELADQIRFV--GLKDMTGAVLGPQEAYYI 261

Query: 303 SERIPHLSLRMKEHCRRAAEFAERMKKAGL--KVIYPGLPDHPQHKLMKRLANSEYGFGG 362
              +    +RM+ HC+ A    + + K     KV YPGL  HP +++ K+       FG 
Sbjct: 262 IRGLKTFEIRMERHCKNARTIVDFLNKHPKVEKVYYPGLETHPGYEIAKKQMKD---FGA 321

Query: 363 MLCLDMGTEERANRLMSVLQNNTQFGFMAVSLGYYETLMSCSGSSTSSEMNREEKQLAGI 422
           M+  ++     A +    L NN +   +AVSLG  ETL+    S T S   +EE+++AGI
Sbjct: 322 MISFELKGGFEAGK---TLLNNLKLCSLAVSLGDTETLIQHPASMTHSPYTKEEREVAGI 381

Query: 423 SPGLIRMSIGYMGTFEQKWSQFEKALAKV 448
           + GL+R+S+G +   E   +  E+ L K+
Sbjct: 382 TDGLVRLSVG-LENVEDIIADLEQGLEKI 395

[Close]

BLAST of MC10g_new0352 vs. ExPASy Swiss-Prot
Match: Q7MX71 (L-methionine gamma-lyase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) OX=242619 GN=mgl PE=1 SV=2)

HSP 1 Score: 204.1 bits (518), Expect = 3.3e-51
Identity = 145/393 (36.90%), Postives = 210/393 (53.44%), Query Frame = 0


Query: 41  AGMAVEDDPAAAIANARHEFGEHGGVNMSIEASATFTVMEPETMRRMFSGELGPDRDFFI 100
           +G A       AI NA       G +   I  ++TF     E   R F+GE     D +I
Sbjct: 9   SGFATRAIHGGAIENA------FGCLATPIYQTSTFVFDTAEQGGRRFAGE----EDGYI 68

Query: 101 YSRHFNPTVLNLSRQMAAMEGTEAAYCTSSGMSAIAAVLLQLLASGDHIVASRTLYGGTH 160
           Y+R  NP    +  ++A +EG EAA   SSG+ AI++ +   + +GDHIVA +TLYG T 
Sbjct: 69  YTRLGNPNCTQVEEKLAMLEGGEAAASASSGIGAISSAIWVCVKAGDHIVAGKTLYGCTF 128

Query: 161 ALLSHFLPRTSGITTSFVDVGNVEMVRAGIVEGKTKVLYFEAVSNPTLAVADVPELCRIG 220
           A L+H L R  G+  + VD  + E V A I    TK++Y E  +NP + + D+  +C I 
Sbjct: 129 AFLTHGLSR-YGVEVTLVDTRHPEEVEAAI-RPNTKLVYLETPANPNMYLTDIKAVCDIA 188

Query: 221 HDH-GLTVVVDNTF-APMVLSPARLGADVVIHSISKFISGGADVIAGAVCGPAKLINSMM 280
           H H G+ V+VDNT+  P +  P  LGAD+V+HS +K+++G  DVIAG V G    I  + 
Sbjct: 189 HKHEGVRVMVDNTYCTPYICRPLELGADIVVHSATKYLNGHGDVIAGFVVGKEDYIKEVK 248

Query: 281 DLRQGSLMLLGPTMNAKVAFELSERIPHLSLRMKEHCRRAAEFAERMKK--AGLKVIYPG 340
            +  G   L G  M+   A+ +S  +  L +RM++HCR A   AE ++K  A   V +PG
Sbjct: 249 LV--GVKDLTGANMSPFDAYLISRGMKTLQIRMEQHCRNAQTVAEFLEKHPAVEAVYFPG 308

Query: 341 LPDHPQHKLMKRLANSEYGFGGMLCLDM-GTEERANRLMSVLQNNTQFGFMAVSLGYYET 400
           LP  PQ++L K+        G M+  ++ G  E   +LM    NN     +AVSLG  ET
Sbjct: 309 LPSFPQYELAKKQMALP---GAMIAFEVKGGCEAGKKLM----NNLHLCSLAVSLGDTET 368

Query: 401 LMSCSGSSTSSEMNREEKQLAGISPGLIRMSIG 429
           L+    S T S    EE+  + IS GL+R+S+G
Sbjct: 369 LIQHPASMTHSPYTPEERAASDISEGLVRLSVG 380

[Close]

BLAST of MC10g_new0352 vs. ExPASy Swiss-Prot
Match: P13254 (L-methionine gamma-lyase OS=Pseudomonas putida OX=303 GN=mdeA PE=1 SV=2)

HSP 1 Score: 192.6 bits (488), Expect = 9.9e-48
Identity = 136/373 (36.46%), Postives = 199/373 (53.35%), Query Frame = 0


Query: 62  EHGGVNM-SIEASATFTVMEPETMRRMFSGELGPDRDFFIYSRHFNPTVLNLSRQMAAME 121
           +HGG  +  +  +ATFT    E     F+GE    +    YSR  NPT+  L  +MA++E
Sbjct: 23  DHGGALVPPVYQTATFTFPTVEYGAACFAGE----QAGHFYSRISNPTLNLLEARMASLE 82

Query: 122 GTEAAYCTSSGMSAIAAVLLQLLASGDHIVASRTLYGGTHALLSHFLPRTSGITTSFVDV 181
           G EA    +SGM AI + L  LL  GD ++   TLYG T A L H +    G+    VD+
Sbjct: 83  GGEAGLALASGMGAITSTLWTLLRPGDEVLLGNTLYGCTFAFLHHGIGE-FGVKLRHVDM 142

Query: 182 GNVEMVRAGIVEGKTKVLYFEAVSNPTLAVADVPELCRIGHDHGLTVVVDNTF-APMVLS 241
            +++ + A +    T+V+YFE+ +NP + +AD+  + +I   HG TVVVDNT+  P +  
Sbjct: 143 ADLQALEAAMTPA-TRVIYFESPANPNMHMADIAGVAKIARKHGATVVVDNTYCTPYLQR 202

Query: 242 PARLGADVVIHSISKFISGGADVIAGAVCGPAKLINSMMDLRQGSLMLLGPTMNAKVAFE 301
           P  LGAD+V+HS +K++SG  D+ AG V G   L++ +    QG   + G  ++   A  
Sbjct: 203 PLELGADLVVHSATKYLSGHGDITAGIVVGSQALVDRIR--LQGLKDMTGAVLSPHDAAL 262

Query: 302 LSERIPHLSLRMKEHCRRA---AEFAERMKKAGLKVIYPGLPDHPQHKLMKRLANSEYGF 361
           L   I  L+LRM  HC  A   AEF  R  +  L + YPGL   PQ+ L ++  +     
Sbjct: 263 LMRGIKTLNLRMDRHCANAQVLAEFLARQPQVEL-IHYPGLASFPQYTLARQQMSQP--- 322

Query: 362 GGMLCLDM-GTEERANRLMSVLQNNTQFGFMAVSLGYYETLMSCSGSSTSSEMNREEKQL 421
           GGM+  ++ G      R M+ LQ  ++    AVSLG  E+L     S T S    EE+  
Sbjct: 323 GGMIAFELKGGIGAGRRFMNALQLFSR----AVSLGDAESLAQHPASMTHSSYTPEERAH 379

Query: 422 AGISPGLIRMSIG 429
            GIS GL+R+S+G
Sbjct: 383 YGISEGLVRLSVG 379

[Close]

BLAST of MC10g_new0352 vs. NCBI nr
Match: NP_001315378.1 (methionine gamma-lyase-like [Cucumis melo] >AGF70153.1 methionine gamma-lyase [Cucumis melo])

HSP 1 Score: 754 bits (1947), Expect = 3.54e-272
Identity = 377/457 (82.49%), Postives = 416/457 (91.03%), Query Frame = 0


Query: 1   MAEIRALNSPLKFSGAKRPGPDDTNGGGNDESYFMSKKSAAGMAVEDDPAAAIANARHEF 60
           MAE++  N    F G KRP PD+++    D      K +A   ++ +DPAAAIAN RHEF
Sbjct: 1   MAELKNHN----FRGTKRPSPDESD----DVKTDSKKPTAMISSLLEDPAAAIANTRHEF 60

Query: 61  GEHGGVNMSIEASATFTVMEPETMRRMFSGELGPDRDFFIYSRHFNPTVLNLSRQMAAME 120
           GEHGGVNMSIEASATFTVMEPETMRRMF+GELGPDRDFFIYSRHFNPTVLNLSRQMAA+E
Sbjct: 61  GEHGGVNMSIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALE 120

Query: 121 GTEAAYCTSSGMSAIAAVLLQLLASGDHIVASRTLYGGTHALLSHFLPRTSGITTSFVDV 180
           GT AAYCTSSGMSAIAAVLLQLLASGDH+VASRTLYGGTHAL++HF PRTS ITT+FVD+
Sbjct: 121 GTAAAYCTSSGMSAIAAVLLQLLASGDHVVASRTLYGGTHALMAHFFPRTSNITTTFVDI 180

Query: 181 GNVEMVRAGIVEGKTKVLYFEAVSNPTLAVADVPELCRIGHDHGLTVVVDNTFAPMVLSP 240
           G+++ V   IVEGKTKVLYFE+VSNPTLAVA++PELCRIGH+ G+TVVVDNTFAPM+LSP
Sbjct: 181 GDLKEVEKAIVEGKTKVLYFESVSNPTLAVANIPELCRIGHEKGVTVVVDNTFAPMILSP 240

Query: 241 ARLGADVVIHSISKFISGGADVIAGAVCGPAKLINSMMDLRQGSLMLLGPTMNAKVAFEL 300
           ARLGADVV+HSISKFISGGAD+IAGAVCGP KL+NSMMDLRQGSLMLLGPTMNAKVAFEL
Sbjct: 241 ARLGADVVVHSISKFISGGADIIAGAVCGPTKLVNSMMDLRQGSLMLLGPTMNAKVAFEL 300

Query: 301 SERIPHLSLRMKEHCRRAAEFAERMKKAGLKVIYPGLPDHPQHKLMKRLANSEYGFGGML 360
           SERIPHLSLRMKEHCRRA+ FAERMKKAGLKVIYPGL DHPQH+LMK LAN EYGFGGML
Sbjct: 301 SERIPHLSLRMKEHCRRASVFAERMKKAGLKVIYPGLEDHPQHQLMKSLANPEYGFGGML 360

Query: 361 CLDMGTEERANRLMSVLQNNTQFGFMAVSLGYYETLMSCSGSSTSSEMNREEKQLAGISP 420
           C+DMGTEERAN+LMS+LQN TQFGFMAVSLGYYETLMSCSGSSTSSEM+ EE++LAGISP
Sbjct: 361 CVDMGTEERANKLMSILQNTTQFGFMAVSLGYYETLMSCSGSSTSSEMSGEERELAGISP 420

Query: 421 GLIRMSIGYMGTFEQKWSQFEKALAKVQDMGLPFCNN 457
           GL+RMSIGYMGT EQKWSQFEKALAKVQD+G+PFCNN
Sbjct: 421 GLVRMSIGYMGTLEQKWSQFEKALAKVQDIGVPFCNN 449

[Close]

BLAST of MC10g_new0352 vs. NCBI nr
Match: XP_038876588.1 (methionine gamma-lyase-like [Benincasa hispida])

HSP 1 Score: 752 bits (1941), Expect = 3.01e-271
Identity = 383/458 (83.62%), Postives = 417/458 (91.05%), Query Frame = 0


Query: 1   MAEIRALNSPLKFSGAKRPGPDDTNGGGNDESYFMSKKSAAGMA-VEDDPAAAIANARHE 60
           MA+++  N    F G KR  PD+++    D     SKKS A ++ + +DPAAAIAN RHE
Sbjct: 1   MAQLKNHN----FCGTKRSAPDESD----DIMKIDSKKSTAMISSLLEDPAAAIANTRHE 60

Query: 61  FGEHGGVNMSIEASATFTVMEPETMRRMFSGELGPDRDFFIYSRHFNPTVLNLSRQMAAM 120
           FGEHGGVNMSIEASATFTVMEPETMRRMFSGELGPDRDFFIYSRHFNPTVLNLSRQMAA+
Sbjct: 61  FGEHGGVNMSIEASATFTVMEPETMRRMFSGELGPDRDFFIYSRHFNPTVLNLSRQMAAL 120

Query: 121 EGTEAAYCTSSGMSAIAAVLLQLLASGDHIVASRTLYGGTHALLSHFLPRTSGITTSFVD 180
           EGT AAYCT+SGMSAIA+VLLQLLASGDH+VASRTLYGGTHALL+HFLPRTS ITTSFVD
Sbjct: 121 EGTAAAYCTASGMSAIASVLLQLLASGDHVVASRTLYGGTHALLTHFLPRTSNITTSFVD 180

Query: 181 VGNVEMVRAGIVEGKTKVLYFEAVSNPTLAVADVPELCRIGHDHGLTVVVDNTFAPMVLS 240
           +G+++ V   IVEGKTKVLYFEAVSNPTLAVAD+PELCRIGH++GLTVVVDNTFAPMVLS
Sbjct: 181 IGDLKEVEKEIVEGKTKVLYFEAVSNPTLAVADIPELCRIGHENGLTVVVDNTFAPMVLS 240

Query: 241 PARLGADVVIHSISKFISGGADVIAGAVCGPAKLINSMMDLRQGSLMLLGPTMNAKVAFE 300
           PARLGADVV+HSISKFISGGAD+IAGAVCGPAKL+NSMMDLRQGSLMLLGPTMNAKVAFE
Sbjct: 241 PARLGADVVVHSISKFISGGADIIAGAVCGPAKLVNSMMDLRQGSLMLLGPTMNAKVAFE 300

Query: 301 LSERIPHLSLRMKEHCRRAAEFAERMKKAGLKVIYPGLPDHPQHKLMKRLANSEYGFGGM 360
           LSERIPHLSLRMKEHCRRA  FAERMK AGLKVIYPGL DHPQH+LMK LAN EYGFGGM
Sbjct: 301 LSERIPHLSLRMKEHCRRALVFAERMKAAGLKVIYPGLEDHPQHQLMKSLANPEYGFGGM 360

Query: 361 LCLDMGTEERANRLMSVLQNNTQFGFMAVSLGYYETLMSCSGSSTSSEMNREEKQLAGIS 420
           LC+DMGTEERAN+LM+VLQN TQFGFMAVSLGYYETLMSCSGSSTSSEM+ EEK+LAGIS
Sbjct: 361 LCIDMGTEERANKLMNVLQNTTQFGFMAVSLGYYETLMSCSGSSTSSEMSGEEKELAGIS 420

Query: 421 PGLIRMSIGYMGTFEQKWSQFEKALAKVQDMGLPFCNN 457
           PGL+RMSIGYMGT +QKWSQFEKALAK+QDMGLP  NN
Sbjct: 421 PGLVRMSIGYMGTLDQKWSQFEKALAKLQDMGLPLSNN 450

[Close]

BLAST of MC10g_new0352 vs. NCBI nr
Match: KAA0052969.1 (methionine gamma-lyase-like [Cucumis melo var. makuwa] >TYK11425.1 methionine gamma-lyase-like [Cucumis melo var. makuwa])

HSP 1 Score: 751 bits (1939), Expect = 5.85e-271
Identity = 376/457 (82.28%), Postives = 415/457 (90.81%), Query Frame = 0


Query: 1   MAEIRALNSPLKFSGAKRPGPDDTNGGGNDESYFMSKKSAAGMAVEDDPAAAIANARHEF 60
           MAE++  N    F G KR  PD+++    D      K +A   ++ +DPAAAIAN RHEF
Sbjct: 1   MAELKNHN----FRGTKRASPDESD----DVKTDSKKPTAMISSLLEDPAAAIANTRHEF 60

Query: 61  GEHGGVNMSIEASATFTVMEPETMRRMFSGELGPDRDFFIYSRHFNPTVLNLSRQMAAME 120
           GEHGGVNMSIEASATFTVMEPETMRRMF+GELGPDRDFFIYSRHFNPTVLNLSRQMAA+E
Sbjct: 61  GEHGGVNMSIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALE 120

Query: 121 GTEAAYCTSSGMSAIAAVLLQLLASGDHIVASRTLYGGTHALLSHFLPRTSGITTSFVDV 180
           GT AAYCTSSGMSAIAAVLLQLLASGDH+VASRTLYGGTHAL++HF PRTS ITT+FVD+
Sbjct: 121 GTAAAYCTSSGMSAIAAVLLQLLASGDHVVASRTLYGGTHALMAHFFPRTSNITTTFVDI 180

Query: 181 GNVEMVRAGIVEGKTKVLYFEAVSNPTLAVADVPELCRIGHDHGLTVVVDNTFAPMVLSP 240
           G+++ V   IVEGKTKVLYFE+VSNPTLAVA++PELCRIGH+ G+TVVVDNTFAPM+LSP
Sbjct: 181 GDLKEVEKAIVEGKTKVLYFESVSNPTLAVANIPELCRIGHEKGVTVVVDNTFAPMILSP 240

Query: 241 ARLGADVVIHSISKFISGGADVIAGAVCGPAKLINSMMDLRQGSLMLLGPTMNAKVAFEL 300
           ARLGADVV+HSISKFISGGAD+IAGAVCGP KL+NSMMDLRQGSLMLLGPTMNAKVAFEL
Sbjct: 241 ARLGADVVVHSISKFISGGADIIAGAVCGPTKLVNSMMDLRQGSLMLLGPTMNAKVAFEL 300

Query: 301 SERIPHLSLRMKEHCRRAAEFAERMKKAGLKVIYPGLPDHPQHKLMKRLANSEYGFGGML 360
           SERIPHLSLRMKEHCRRA+ FAERMKKAGLKVIYPGL DHPQH+LMK LAN EYGFGGML
Sbjct: 301 SERIPHLSLRMKEHCRRASVFAERMKKAGLKVIYPGLEDHPQHQLMKSLANPEYGFGGML 360

Query: 361 CLDMGTEERANRLMSVLQNNTQFGFMAVSLGYYETLMSCSGSSTSSEMNREEKQLAGISP 420
           C+DMGTEERAN+LMS+LQN TQFGFMAVSLGYYETLMSCSGSSTSSEM+ EE++LAGISP
Sbjct: 361 CVDMGTEERANKLMSILQNTTQFGFMAVSLGYYETLMSCSGSSTSSEMSGEERELAGISP 420

Query: 421 GLIRMSIGYMGTFEQKWSQFEKALAKVQDMGLPFCNN 457
           GL+RMSIGYMGT EQKWSQFEKALAKVQD+G+PFCNN
Sbjct: 421 GLVRMSIGYMGTLEQKWSQFEKALAKVQDIGVPFCNN 449

[Close]

BLAST of MC10g_new0352 vs. NCBI nr
Match: XP_023553436.1 (methionine gamma-lyase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 749 bits (1934), Expect = 3.38e-270
Identity = 378/444 (85.14%), Postives = 409/444 (92.12%), Query Frame = 0


Query: 15  GAKRPGPDDTNGGGNDESYFMSKKSAAGM-AVEDDPAAAIANARHEFGEHGGVNMSIEAS 74
           GAKR   D+T     +   F  KKS A + ++ +DPAAAIAN RHEFGEHGGVNMSIEAS
Sbjct: 11  GAKRSASDET-----EAIKFDPKKSTAMVPSLLEDPAAAIANTRHEFGEHGGVNMSIEAS 70

Query: 75  ATFTVMEPETMRRMFSGELGPDRDFFIYSRHFNPTVLNLSRQMAAMEGTEAAYCTSSGMS 134
           ATFTVMEPETMRRMF+GELGPDRDFFIYSRHFNPTVLNLSRQMAA+EGT AAYCTSSGMS
Sbjct: 71  ATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTSSGMS 130

Query: 135 AIAAVLLQLLASGDHIVASRTLYGGTHALLSHFLPRTSGITTSFVDVGNVEMVRAGIVEG 194
           AI AVLLQLLASGDH+VASRTLYGGTHALL+HFLPRTS ITTSFVD+G++E V   IVEG
Sbjct: 131 AICAVLLQLLASGDHVVASRTLYGGTHALLAHFLPRTSNITTSFVDIGDLEEVEKEIVEG 190

Query: 195 KTKVLYFEAVSNPTLAVADVPELCRIGHDHGLTVVVDNTFAPMVLSPARLGADVVIHSIS 254
           KTKVLYFEAVSNPTLAVA++PELCRIGH  GLTVVVDNTF PMVLSPARLGADVV+HSIS
Sbjct: 191 KTKVLYFEAVSNPTLAVANIPELCRIGHAKGLTVVVDNTFTPMVLSPARLGADVVVHSIS 250

Query: 255 KFISGGADVIAGAVCGPAKLINSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLSLRMKE 314
           KFISGGAD+IAGAVCGP+KL+NSMMDLRQGSLMLLGPTMNAKVAFELSERIPHL LRMKE
Sbjct: 251 KFISGGADIIAGAVCGPSKLVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLGLRMKE 310

Query: 315 HCRRAAEFAERMKKAGLKVIYPGLPDHPQHKLMKRLANSEYGFGGMLCLDMGTEERANRL 374
           HCRRAAEFAERMKKAGLKVIYPGLPDHPQH+LMK L NSEYGFGGMLCLDMGTEE+AN+L
Sbjct: 311 HCRRAAEFAERMKKAGLKVIYPGLPDHPQHQLMKSLVNSEYGFGGMLCLDMGTEEKANKL 370

Query: 375 MSVLQNNTQFGFMAVSLGYYETLMSCSGSSTSSEMNREEKQLAGISPGLIRMSIGYMGTF 434
           M++LQN+TQFGFMAVSLGYYETLMSCSGSSTSSEM+REEK+LAGISPGLIRMS+GY+GT 
Sbjct: 371 MNILQNSTQFGFMAVSLGYYETLMSCSGSSTSSEMSREEKELAGISPGLIRMSVGYIGTL 430

Query: 435 EQKWSQFEKALAKVQDMGLPFCNN 457
           EQKWSQFEKA+AKV+   LPFCNN
Sbjct: 431 EQKWSQFEKAIAKVEGKELPFCNN 449

[Close]

BLAST of MC10g_new0352 vs. NCBI nr
Match: KAG7015808.1 (Methionine gamma-lyase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 748 bits (1930), Expect = 1.37e-269
Identity = 377/444 (84.91%), Postives = 409/444 (92.12%), Query Frame = 0


Query: 15  GAKRPGPDDTNGGGNDESYFMSKKSAAGM-AVEDDPAAAIANARHEFGEHGGVNMSIEAS 74
           GAKR   D+T     +   F  KKS A + ++ +DPAAAIAN RHEFGEHGGVNMSIEAS
Sbjct: 11  GAKRSASDET-----EAIKFDPKKSTAMVPSLLEDPAAAIANTRHEFGEHGGVNMSIEAS 70

Query: 75  ATFTVMEPETMRRMFSGELGPDRDFFIYSRHFNPTVLNLSRQMAAMEGTEAAYCTSSGMS 134
           ATFTVMEPETMRRMF+GELGPDRDF+IYSRHFNPTVLNLSRQMAA+EGT AAYCTSSGMS
Sbjct: 71  ATFTVMEPETMRRMFAGELGPDRDFYIYSRHFNPTVLNLSRQMAALEGTAAAYCTSSGMS 130

Query: 135 AIAAVLLQLLASGDHIVASRTLYGGTHALLSHFLPRTSGITTSFVDVGNVEMVRAGIVEG 194
           AI AVLLQLLASGDH+VASRTLYGGTHALL+HFLPRTS ITTSFVD+G++E V   IVEG
Sbjct: 131 AICAVLLQLLASGDHVVASRTLYGGTHALLAHFLPRTSNITTSFVDIGDLEEVEKEIVEG 190

Query: 195 KTKVLYFEAVSNPTLAVADVPELCRIGHDHGLTVVVDNTFAPMVLSPARLGADVVIHSIS 254
           KTKVLYFEAVSNPTLAVA++PELCRIGH  GLTVVVDNTF PMVLSPARLGADVV+HSIS
Sbjct: 191 KTKVLYFEAVSNPTLAVANIPELCRIGHAKGLTVVVDNTFTPMVLSPARLGADVVVHSIS 250

Query: 255 KFISGGADVIAGAVCGPAKLINSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLSLRMKE 314
           KFISGGAD+IAGAVCGP+KL+NSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLSLRMKE
Sbjct: 251 KFISGGADIIAGAVCGPSKLVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLSLRMKE 310

Query: 315 HCRRAAEFAERMKKAGLKVIYPGLPDHPQHKLMKRLANSEYGFGGMLCLDMGTEERANRL 374
           HCRRAAEFAERMKKAGLKVIYPGLPDHPQH+LMK L NSEYGFGGMLCLDMGTEE+AN+L
Sbjct: 311 HCRRAAEFAERMKKAGLKVIYPGLPDHPQHQLMKSLVNSEYGFGGMLCLDMGTEEKANKL 370

Query: 375 MSVLQNNTQFGFMAVSLGYYETLMSCSGSSTSSEMNREEKQLAGISPGLIRMSIGYMGTF 434
           M++LQN TQFGFMAVSLGYYETLMSCSGSSTSSEM+REEK+LAGISPGLIRMS+GY+GT 
Sbjct: 371 MNILQNTTQFGFMAVSLGYYETLMSCSGSSTSSEMSREEKELAGISPGLIRMSVGYIGTL 430

Query: 435 EQKWSQFEKALAKVQDMGLPFCNN 457
           +QKWSQFEKA+AKV+   LPFCNN
Sbjct: 431 DQKWSQFEKAIAKVEAKELPFCNN 449

[Close]

BLAST of MC10g_new0352 vs. ExPASy TrEMBL
Match: M1NFB7 (Methionine gamma-lyase OS=Cucumis melo OX=3656 GN=MGL PE=2 SV=1)

HSP 1 Score: 754 bits (1947), Expect = 1.71e-272
Identity = 377/457 (82.49%), Postives = 416/457 (91.03%), Query Frame = 0


Query: 1   MAEIRALNSPLKFSGAKRPGPDDTNGGGNDESYFMSKKSAAGMAVEDDPAAAIANARHEF 60
           MAE++  N    F G KRP PD+++    D      K +A   ++ +DPAAAIAN RHEF
Sbjct: 1   MAELKNHN----FRGTKRPSPDESD----DVKTDSKKPTAMISSLLEDPAAAIANTRHEF 60

Query: 61  GEHGGVNMSIEASATFTVMEPETMRRMFSGELGPDRDFFIYSRHFNPTVLNLSRQMAAME 120
           GEHGGVNMSIEASATFTVMEPETMRRMF+GELGPDRDFFIYSRHFNPTVLNLSRQMAA+E
Sbjct: 61  GEHGGVNMSIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALE 120

Query: 121 GTEAAYCTSSGMSAIAAVLLQLLASGDHIVASRTLYGGTHALLSHFLPRTSGITTSFVDV 180
           GT AAYCTSSGMSAIAAVLLQLLASGDH+VASRTLYGGTHAL++HF PRTS ITT+FVD+
Sbjct: 121 GTAAAYCTSSGMSAIAAVLLQLLASGDHVVASRTLYGGTHALMAHFFPRTSNITTTFVDI 180

Query: 181 GNVEMVRAGIVEGKTKVLYFEAVSNPTLAVADVPELCRIGHDHGLTVVVDNTFAPMVLSP 240
           G+++ V   IVEGKTKVLYFE+VSNPTLAVA++PELCRIGH+ G+TVVVDNTFAPM+LSP
Sbjct: 181 GDLKEVEKAIVEGKTKVLYFESVSNPTLAVANIPELCRIGHEKGVTVVVDNTFAPMILSP 240

Query: 241 ARLGADVVIHSISKFISGGADVIAGAVCGPAKLINSMMDLRQGSLMLLGPTMNAKVAFEL 300
           ARLGADVV+HSISKFISGGAD+IAGAVCGP KL+NSMMDLRQGSLMLLGPTMNAKVAFEL
Sbjct: 241 ARLGADVVVHSISKFISGGADIIAGAVCGPTKLVNSMMDLRQGSLMLLGPTMNAKVAFEL 300

Query: 301 SERIPHLSLRMKEHCRRAAEFAERMKKAGLKVIYPGLPDHPQHKLMKRLANSEYGFGGML 360
           SERIPHLSLRMKEHCRRA+ FAERMKKAGLKVIYPGL DHPQH+LMK LAN EYGFGGML
Sbjct: 301 SERIPHLSLRMKEHCRRASVFAERMKKAGLKVIYPGLEDHPQHQLMKSLANPEYGFGGML 360

Query: 361 CLDMGTEERANRLMSVLQNNTQFGFMAVSLGYYETLMSCSGSSTSSEMNREEKQLAGISP 420
           C+DMGTEERAN+LMS+LQN TQFGFMAVSLGYYETLMSCSGSSTSSEM+ EE++LAGISP
Sbjct: 361 CVDMGTEERANKLMSILQNTTQFGFMAVSLGYYETLMSCSGSSTSSEMSGEERELAGISP 420

Query: 421 GLIRMSIGYMGTFEQKWSQFEKALAKVQDMGLPFCNN 457
           GL+RMSIGYMGT EQKWSQFEKALAKVQD+G+PFCNN
Sbjct: 421 GLVRMSIGYMGTLEQKWSQFEKALAKVQDIGVPFCNN 449

[Close]

BLAST of MC10g_new0352 vs. ExPASy TrEMBL
Match: A0A5A7UHF4 (Methionine gamma-lyase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00790 PE=3 SV=1)

HSP 1 Score: 751 bits (1939), Expect = 2.83e-271
Identity = 376/457 (82.28%), Postives = 415/457 (90.81%), Query Frame = 0


Query: 1   MAEIRALNSPLKFSGAKRPGPDDTNGGGNDESYFMSKKSAAGMAVEDDPAAAIANARHEF 60
           MAE++  N    F G KR  PD+++    D      K +A   ++ +DPAAAIAN RHEF
Sbjct: 1   MAELKNHN----FRGTKRASPDESD----DVKTDSKKPTAMISSLLEDPAAAIANTRHEF 60

Query: 61  GEHGGVNMSIEASATFTVMEPETMRRMFSGELGPDRDFFIYSRHFNPTVLNLSRQMAAME 120
           GEHGGVNMSIEASATFTVMEPETMRRMF+GELGPDRDFFIYSRHFNPTVLNLSRQMAA+E
Sbjct: 61  GEHGGVNMSIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALE 120

Query: 121 GTEAAYCTSSGMSAIAAVLLQLLASGDHIVASRTLYGGTHALLSHFLPRTSGITTSFVDV 180
           GT AAYCTSSGMSAIAAVLLQLLASGDH+VASRTLYGGTHAL++HF PRTS ITT+FVD+
Sbjct: 121 GTAAAYCTSSGMSAIAAVLLQLLASGDHVVASRTLYGGTHALMAHFFPRTSNITTTFVDI 180

Query: 181 GNVEMVRAGIVEGKTKVLYFEAVSNPTLAVADVPELCRIGHDHGLTVVVDNTFAPMVLSP 240
           G+++ V   IVEGKTKVLYFE+VSNPTLAVA++PELCRIGH+ G+TVVVDNTFAPM+LSP
Sbjct: 181 GDLKEVEKAIVEGKTKVLYFESVSNPTLAVANIPELCRIGHEKGVTVVVDNTFAPMILSP 240

Query: 241 ARLGADVVIHSISKFISGGADVIAGAVCGPAKLINSMMDLRQGSLMLLGPTMNAKVAFEL 300
           ARLGADVV+HSISKFISGGAD+IAGAVCGP KL+NSMMDLRQGSLMLLGPTMNAKVAFEL
Sbjct: 241 ARLGADVVVHSISKFISGGADIIAGAVCGPTKLVNSMMDLRQGSLMLLGPTMNAKVAFEL 300

Query: 301 SERIPHLSLRMKEHCRRAAEFAERMKKAGLKVIYPGLPDHPQHKLMKRLANSEYGFGGML 360
           SERIPHLSLRMKEHCRRA+ FAERMKKAGLKVIYPGL DHPQH+LMK LAN EYGFGGML
Sbjct: 301 SERIPHLSLRMKEHCRRASVFAERMKKAGLKVIYPGLEDHPQHQLMKSLANPEYGFGGML 360

Query: 361 CLDMGTEERANRLMSVLQNNTQFGFMAVSLGYYETLMSCSGSSTSSEMNREEKQLAGISP 420
           C+DMGTEERAN+LMS+LQN TQFGFMAVSLGYYETLMSCSGSSTSSEM+ EE++LAGISP
Sbjct: 361 CVDMGTEERANKLMSILQNTTQFGFMAVSLGYYETLMSCSGSSTSSEMSGEERELAGISP 420

Query: 421 GLIRMSIGYMGTFEQKWSQFEKALAKVQDMGLPFCNN 457
           GL+RMSIGYMGT EQKWSQFEKALAKVQD+G+PFCNN
Sbjct: 421 GLVRMSIGYMGTLEQKWSQFEKALAKVQDIGVPFCNN 449

[Close]

BLAST of MC10g_new0352 vs. ExPASy TrEMBL
Match: A0A1S3BKU9 (methionine gamma-lyase-like OS=Cucumis melo OX=3656 GN=LOC103490704 PE=3 SV=1)

HSP 1 Score: 751 bits (1939), Expect = 2.83e-271
Identity = 376/457 (82.28%), Postives = 415/457 (90.81%), Query Frame = 0


Query: 1   MAEIRALNSPLKFSGAKRPGPDDTNGGGNDESYFMSKKSAAGMAVEDDPAAAIANARHEF 60
           MAE++  N    F G KR  PD+++    D      K +A   ++ +DPAAAIAN RHEF
Sbjct: 1   MAELKNHN----FRGTKRASPDESD----DVKTDSKKPTAMISSLLEDPAAAIANTRHEF 60

Query: 61  GEHGGVNMSIEASATFTVMEPETMRRMFSGELGPDRDFFIYSRHFNPTVLNLSRQMAAME 120
           GEHGGVNMSIEASATFTVMEPETMRRMF+GELGPDRDFFIYSRHFNPTVLNLSRQMAA+E
Sbjct: 61  GEHGGVNMSIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALE 120

Query: 121 GTEAAYCTSSGMSAIAAVLLQLLASGDHIVASRTLYGGTHALLSHFLPRTSGITTSFVDV 180
           GT AAYCTSSGMSAIAAVLLQLLASGDH+VASRTLYGGTHAL++HF PRTS ITT+FVD+
Sbjct: 121 GTAAAYCTSSGMSAIAAVLLQLLASGDHVVASRTLYGGTHALMAHFFPRTSNITTTFVDI 180

Query: 181 GNVEMVRAGIVEGKTKVLYFEAVSNPTLAVADVPELCRIGHDHGLTVVVDNTFAPMVLSP 240
           G+++ V   IVEGKTKVLYFE+VSNPTLAVA++PELCRIGH+ G+TVVVDNTFAPM+LSP
Sbjct: 181 GDLKEVEKAIVEGKTKVLYFESVSNPTLAVANIPELCRIGHEKGVTVVVDNTFAPMILSP 240

Query: 241 ARLGADVVIHSISKFISGGADVIAGAVCGPAKLINSMMDLRQGSLMLLGPTMNAKVAFEL 300
           ARLGADVV+HSISKFISGGAD+IAGAVCGP KL+NSMMDLRQGSLMLLGPTMNAKVAFEL
Sbjct: 241 ARLGADVVVHSISKFISGGADIIAGAVCGPTKLVNSMMDLRQGSLMLLGPTMNAKVAFEL 300

Query: 301 SERIPHLSLRMKEHCRRAAEFAERMKKAGLKVIYPGLPDHPQHKLMKRLANSEYGFGGML 360
           SERIPHLSLRMKEHCRRA+ FAERMKKAGLKVIYPGL DHPQH+LMK LAN EYGFGGML
Sbjct: 301 SERIPHLSLRMKEHCRRASVFAERMKKAGLKVIYPGLEDHPQHQLMKSLANPEYGFGGML 360

Query: 361 CLDMGTEERANRLMSVLQNNTQFGFMAVSLGYYETLMSCSGSSTSSEMNREEKQLAGISP 420
           C+DMGTEERAN+LMS+LQN TQFGFMAVSLGYYETLMSCSGSSTSSEM+ EE++LAGISP
Sbjct: 361 CVDMGTEERANKLMSILQNTTQFGFMAVSLGYYETLMSCSGSSTSSEMSGEERELAGISP 420

Query: 421 GLIRMSIGYMGTFEQKWSQFEKALAKVQDMGLPFCNN 457
           GL+RMSIGYMGT EQKWSQFEKALAKVQD+G+PFCNN
Sbjct: 421 GLVRMSIGYMGTLEQKWSQFEKALAKVQDIGVPFCNN 449

[Close]

BLAST of MC10g_new0352 vs. ExPASy TrEMBL
Match: A0A0A0L129 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G002890 PE=3 SV=1)

HSP 1 Score: 748 bits (1930), Expect = 6.90e-270
Identity = 373/446 (83.63%), Postives = 410/446 (91.93%), Query Frame = 0


Query: 13  FSGAKRPGPDDTNGGGNDESYFMSKKSAAGMA-VEDDPAAAIANARHEFGEHGGVNMSIE 72
           F G KRP  D    G +D     SKK  A ++ + +DPAAAIAN RHEFGEHGGVNMSIE
Sbjct: 9   FRGTKRPSSD----GSDDVKLADSKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMSIE 68

Query: 73  ASATFTVMEPETMRRMFSGELGPDRDFFIYSRHFNPTVLNLSRQMAAMEGTEAAYCTSSG 132
           ASATFTVMEPETMRRMF+GELGPDRDFFIYSRHFNPTVLNLSRQMAA+EGT AAYCTSSG
Sbjct: 69  ASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTSSG 128

Query: 133 MSAIAAVLLQLLASGDHIVASRTLYGGTHALLSHFLPRTSGITTSFVDVGNVEMVRAGIV 192
           MSAIAAVLLQLLASGDH+VASRTLYGGTHAL++HF PRTS ITT+FVD+G+++ V   IV
Sbjct: 129 MSAIAAVLLQLLASGDHVVASRTLYGGTHALMAHFFPRTSNITTTFVDIGDLKEVEKAIV 188

Query: 193 EGKTKVLYFEAVSNPTLAVADVPELCRIGHDHGLTVVVDNTFAPMVLSPARLGADVVIHS 252
           EGKTKVLYFE+VSNPTLAVA++PELCRIGH+ G+TVVVDNTFAPM+LSPARLGADVV+HS
Sbjct: 189 EGKTKVLYFESVSNPTLAVANIPELCRIGHEKGVTVVVDNTFAPMILSPARLGADVVVHS 248

Query: 253 ISKFISGGADVIAGAVCGPAKLINSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLSLRM 312
           ISKFISGGAD+IAGAVCGP KL+NSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLSLRM
Sbjct: 249 ISKFISGGADIIAGAVCGPTKLVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLSLRM 308

Query: 313 KEHCRRAAEFAERMKKAGLKVIYPGLPDHPQHKLMKRLANSEYGFGGMLCLDMGTEERAN 372
           KEHCRRA+ FAERMKKAGLKV+YPGLPDHPQH+LMK LA+ EYGFGGMLC+DMGTEE+AN
Sbjct: 309 KEHCRRASVFAERMKKAGLKVVYPGLPDHPQHQLMKSLASPEYGFGGMLCVDMGTEEKAN 368

Query: 373 RLMSVLQNNTQFGFMAVSLGYYETLMSCSGSSTSSEMNREEKQLAGISPGLIRMSIGYMG 432
           +LMS+LQN TQFGFMAVSLGYYETLMSCSGSSTSSEM+ EE++LAGISPGL+RMSIGYMG
Sbjct: 369 KLMSILQNTTQFGFMAVSLGYYETLMSCSGSSTSSEMSGEERELAGISPGLVRMSIGYMG 428

Query: 433 TFEQKWSQFEKALAKVQDMGLPFCNN 457
           T EQKWSQFEKALAKVQD G+PFCNN
Sbjct: 429 TLEQKWSQFEKALAKVQDNGVPFCNN 450

[Close]

BLAST of MC10g_new0352 vs. ExPASy TrEMBL
Match: A0A6J1EC60 (methionine gamma-lyase-like OS=Cucurbita moschata OX=3662 GN=LOC111431210 PE=3 SV=1)

HSP 1 Score: 746 bits (1927), Expect = 1.90e-269
Identity = 376/444 (84.68%), Postives = 409/444 (92.12%), Query Frame = 0


Query: 15  GAKRPGPDDTNGGGNDESYFMSKKSAAGM-AVEDDPAAAIANARHEFGEHGGVNMSIEAS 74
           GAKR   D+T     +   F  KKS A + ++ +DPAAAIAN RHEFGEHGGVNMSIEAS
Sbjct: 11  GAKRSASDET-----EAIKFDPKKSTAMVPSLLEDPAAAIANTRHEFGEHGGVNMSIEAS 70

Query: 75  ATFTVMEPETMRRMFSGELGPDRDFFIYSRHFNPTVLNLSRQMAAMEGTEAAYCTSSGMS 134
           ATFTVMEPETMRRMF+GELGPDRDF+IYSRHFNPTVLNLSRQMAA+EGT AAYCTSSGMS
Sbjct: 71  ATFTVMEPETMRRMFAGELGPDRDFYIYSRHFNPTVLNLSRQMAALEGTAAAYCTSSGMS 130

Query: 135 AIAAVLLQLLASGDHIVASRTLYGGTHALLSHFLPRTSGITTSFVDVGNVEMVRAGIVEG 194
           AI AVLLQLLASGDH+VASRTLYGGTHALL+HFLPRTS ITTSFVD+G++E V   IVEG
Sbjct: 131 AICAVLLQLLASGDHVVASRTLYGGTHALLAHFLPRTSNITTSFVDIGDLEEVEKEIVEG 190

Query: 195 KTKVLYFEAVSNPTLAVADVPELCRIGHDHGLTVVVDNTFAPMVLSPARLGADVVIHSIS 254
           KTKVLYFEAVSNPTLAVA++PELCRIGH  GLTVVVDNTF PMVLSPARLGADVV+HSIS
Sbjct: 191 KTKVLYFEAVSNPTLAVANIPELCRIGHAKGLTVVVDNTFTPMVLSPARLGADVVVHSIS 250

Query: 255 KFISGGADVIAGAVCGPAKLINSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLSLRMKE 314
           KFISGGAD+IAGAVCGP+KL+NSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLSLRMKE
Sbjct: 251 KFISGGADIIAGAVCGPSKLVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLSLRMKE 310

Query: 315 HCRRAAEFAERMKKAGLKVIYPGLPDHPQHKLMKRLANSEYGFGGMLCLDMGTEERANRL 374
           HC+RAAEFAERMKKAGLKVIYPGLPDHPQH+LMK L NSEYGFGGMLCLDMGTEE+AN+L
Sbjct: 311 HCKRAAEFAERMKKAGLKVIYPGLPDHPQHQLMKSLVNSEYGFGGMLCLDMGTEEKANKL 370

Query: 375 MSVLQNNTQFGFMAVSLGYYETLMSCSGSSTSSEMNREEKQLAGISPGLIRMSIGYMGTF 434
           M++LQN TQFGFMAVSLGYYETLMSCSGSSTSSEM+REEK+LAGISPGLIRMS+GY+GT 
Sbjct: 371 MNILQNTTQFGFMAVSLGYYETLMSCSGSSTSSEMSREEKELAGISPGLIRMSVGYIGTL 430

Query: 435 EQKWSQFEKALAKVQDMGLPFCNN 457
           +QKWSQFEKA+AKV+   LPFCNN
Sbjct: 431 DQKWSQFEKAIAKVEAKELPFCNN 449

[Close]

BLAST of MC10g_new0352 vs. TAIR 10
Match: AT1G64660.1 (methionine gamma-lyase )

HSP 1 Score: 644.0 bits (1660), Expect = 8.8e-185
Identity = 321/447 (71.81%), Postives = 374/447 (83.67%), Query Frame = 0


Query: 1   MAEIRALNSPLKFSGAKRPGPDDTNGGGNDESYFMSKKSAAGMAVEDDPAAAIANARHEF 60
           MA       PL FSG KR   DD +G        ++KKSA  +  + DPAAAIAN RHEF
Sbjct: 1   MAHFLETQEPLVFSGKKRNDRDDEDGDA-----LVAKKSALAVC-DADPAAAIANIRHEF 60

Query: 61  GEHGGVNMSIEASATFTVMEPETMRRMFSGELGPDRDFFIYSRHFNPTVLNLSRQMAAME 120
           GEHGGVNMSIEASATFTVMEP+TMRRMF+GELGPD DF++YSRHFNPTVLNLSRQMAA+E
Sbjct: 61  GEHGGVNMSIEASATFTVMEPDTMRRMFTGELGPDNDFYVYSRHFNPTVLNLSRQMAALE 120

Query: 121 GTEAAYCTSSGMSAIAAVLLQLLASGDHIVASRTLYGGTHALLSHFLPRTSGITTSFVDV 180
           GT+AAYCTSSGMSAI++V+LQL +SG H+VA+ TLYGGTHALLSHFLPRT  ITTSFVD+
Sbjct: 121 GTQAAYCTSSGMSAISSVMLQLCSSGGHVVAASTLYGGTHALLSHFLPRTCNITTSFVDI 180

Query: 181 GNVEMVRAGIVEGKTKVLYFEAVSNPTLAVADVPELCRIGHDHGLTVVVDNTFAPMVLSP 240
            +   V   IVEG+T+VLYFE+V+NPTL VAD+PEL R+ H+ G+TVVVDNTFAPMVLSP
Sbjct: 181 TDHGAVANAIVEGRTQVLYFESVANPTLTVADIPELSRMAHEKGVTVVVDNTFAPMVLSP 240

Query: 241 ARLGADVVIHSISKFISGGADVIAGAVCGPAKLINSMMDLRQGSLMLLGPTMNAKVAFEL 300
           A+LGADVV+HSISKFISGGAD+IAGAVCG   L+  MMDLR GSLMLLGPTMNAKVAFEL
Sbjct: 241 AKLGADVVVHSISKFISGGADIIAGAVCGSENLVKEMMDLRGGSLMLLGPTMNAKVAFEL 300

Query: 301 SERIPHLSLRMKEHCRRAAEFAERMKKAGLKVIYPGLPDHPQHKLMKRLANSEYGFGGML 360
           SERIPHL LRM+EH  RA  +AERM+  G+KVIYPGL  HPQHKL K + N +YG+GG+L
Sbjct: 301 SERIPHLGLRMREHSHRAQVYAERMRDLGMKVIYPGLETHPQHKLFKGMVNRDYGYGGLL 360

Query: 361 CLDMGTEERANRLMSVLQNNTQFGFMAVSLGYYETLMSCSGSSTSSEMNREEKQLAGISP 420
            +DM TEE+AN+LM+ LQN TQFGFMAVSLGYYETLMSCSGSSTSSE++  +K+ AGISP
Sbjct: 361 SIDMETEEKANKLMAYLQNATQFGFMAVSLGYYETLMSCSGSSTSSELDPSQKEAAGISP 420

Query: 421 GLIRMSIGYMGTFEQKWSQFEKALAKV 448
           GL+RMS+GY+GT EQKW+QFEKA  ++
Sbjct: 421 GLVRMSVGYVGTLEQKWTQFEKAFLRM 441

[Close]

BLAST of MC10g_new0352 vs. TAIR 10
Match: AT3G01120.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 172.6 bits (436), Expect = 7.5e-43
Identity = 111/335 (33.13%), Postives = 179/335 (53.43%), Query Frame = 0


Query: 99  FIYSRHFNPTVLNLSRQMAAMEGTEAAYCTSSGMSAIAAVLLQLLASGDHIVASRTLYGG 158
           F Y R+ NPT + L  +++A+EG E+    +SGM A   +LL L+ +G HIV +   Y  
Sbjct: 224 FEYGRYGNPTTVVLEDKISALEGAESTLVMASGMCASTVMLLALVPAGGHIVTTTDCYRK 283

Query: 159 THALLSHFLPRTSGITTSFVDVGNVEMVRAGIVEGKTKVLYFEAVSNPTLAVADVPELCR 218
           T   + +FLP+  GIT + +D  ++  + A + E K  + + E+ +NP L   D+  + +
Sbjct: 284 TRIFMENFLPKL-GITVTVIDPADIAGLEAAVNEFKVSLFFTESPTNPFLRCVDIELVSK 343

Query: 219 IGHDHGLTVVVDNTFA-PMVLSPARLGADVVIHSISKFISGGADVIAGAVCGPAKLINSM 278
           I H  G  V +D TFA P+      LGAD+V+HS +K+I G  DV+AG +CG  KL++ +
Sbjct: 344 ICHKRGTLVCIDGTFATPLNQKALALGADLVVHSATKYIGGHNDVLAGCICGSLKLVSEI 403

Query: 279 MDLRQGSLMLLGPTMNAKVAFELSERIPHLSLRMKEH---CRRAAEFAERMKKAGLKVIY 338
            +L      +LG T+N   A+ +   +  L LR+++      R AE  E   K    V Y
Sbjct: 404 RNLHH----VLGGTLNPNAAYLIIRGMKTLHLRVQQQNSTAFRMAEILEAHPKVS-HVYY 463

Query: 339 PGLPDHPQHKLMKRLANSEYGFGGMLCLDM-GTEERANRLMSVLQNNTQFGFMAVSLGYY 398
           PGLP HP+H+L KR      GFGG++  ++ G  E   + +  L    +  ++A S G  
Sbjct: 464 PGLPSHPEHELAKRQMT---GFGGVVSFEIDGDIETTIKFVDSL----KIPYIAPSFGGC 523

Query: 399 ETLMSCSGSSTSSEMNREEKQLAGISPGLIRMSIG 429
           E+++      +  ++ +EE+   GI   L+R S G
Sbjct: 524 ESIVDQPAIMSYWDLPQEERLKYGIKDNLVRFSFG 545

[Close]

BLAST of MC10g_new0352 vs. TAIR 10
Match: AT1G33320.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 143.7 bits (361), Expect = 3.8e-34
Identity = 97/336 (28.87%), Postives = 175/336 (52.08%), Query Frame = 0


Query: 97  DFFIYSRHFNPTVLNLSRQMAAMEGTEAAYCTSSGMSAIAAVLLQLLASGDHIVASRTLY 156
           D + Y+R+ NPT + L  +++ +EG E+    +SGM A   +LL L+ +  HIVA++  Y
Sbjct: 72  DLYEYARYGNPTTMALEEKISVLEGAESTLVMASGMYASNVMLLALVPTNGHIVATKDCY 131

Query: 157 GGTHALLSHFLPRTSGITTSFVDVGNVEMVRAGIVEGKTKVLYFEAVSNPTLAVADVPEL 216
             T   + +FL +  GIT +F+D  ++  ++  +   +  + + E+ +NP L   D+  +
Sbjct: 132 KETRIFMENFLTKL-GITVTFIDSDDIAGLQTLVNNHEVSLFFTESPTNPFLRCVDIKLV 191

Query: 217 CRIGHDHGLTVVVDNTFA-PMVLSPARLGADVVIHSISKFISGGADVIAGAVCGPAKLIN 276
            +I H  G  V +D T A P+      LGAD+V HS +K+I G  D +AG++ G  +L++
Sbjct: 192 SKICHRRGTLVCIDATIATPINQKTLALGADLVHHSATKYIGGHNDFLAGSISGSMELVS 251

Query: 277 SMMDLRQGSLMLLGPTMNAKVAFELSERIPHLSLRMKEHCRRAAEFAERMK--KAGLKVI 336
            + +L +    LLG T+N   A+ L   +  + LR+++      + A+ ++      +V 
Sbjct: 252 KIRNLHK----LLGGTLNPNAAYLLIRGMKTMHLRVRQQNSTGMKMAQVLEAHPKVSRVY 311

Query: 337 YPGLPDHPQHKLMKRLANSEYGFGGMLCLDM-GTEERANRLMSVLQNNTQFGFMAVSLGY 396
           Y GLP HP+H + KR      G GG++  ++ G  +   + +  L    +  ++A S G 
Sbjct: 312 YLGLPSHPEHLIAKRQMT---GIGGLISFEIDGDLKTTIKFIDAL----KIPYLAASFGG 371

Query: 397 YETLMSCSGSSTSSEMNREEKQLAGISPGLIRMSIG 429
            E+L+    +    ++ REE+   G    L+R S G
Sbjct: 372 CESLVDQLATGI-WDIPREERLKDGFQDNLVRFSFG 394

[Close]

BLAST of MC10g_new0352 vs. TAIR 10
Match: AT3G57050.2 (cystathionine beta-lyase )

HSP 1 Score: 141.0 bits (354), Expect = 2.4e-33
Identity = 115/398 (28.89%), Postives = 205/398 (51.51%), Query Frame = 0


Query: 37  KKSAAGMAVEDDPAAA--IANARHEFGEHGGVNMSIEASATFTVMEPETMRRMFSGELGP 96
           +KS   M ++++ + +  + N  ++F     ++  +  +ATF   +P  +      E GP
Sbjct: 58  EKSVDAMNIKEEASVSTLLVNLDNKFDPFDAMSTPLYQTATF--KQPSAI------ENGP 117

Query: 97  DRDFFIYSRHFNPTVLNLSRQMAAMEGTEAAYCTSSGMSAIAAVLLQLLASGDHIVASRT 156
               + Y+R  NPT   L   +A ++  + A+C +SGM+A++AV   L+ +G+ IVA   
Sbjct: 118 ----YDYTRSGNPTRDALESLLAKLDKADRAFCFTSGMAALSAV-THLIKNGEEIVAGDD 177

Query: 157 LYGGTHALLSHFLPRTSGITTSFVDVGNVEMVRAGIVEGKTKVLYFEAVSNPTLAVADVP 216
           +YGG+  LLS  +PR SG+    V+   ++ V A I   +TK+++ E+ +NP   ++D+ 
Sbjct: 178 VYGGSDRLLSQVVPR-SGVVVKRVNTTKLDEVAAAI-GPQTKLVWLESPTNPRQQISDIR 237

Query: 217 ELCRIGHDHGLTVVVDNTFAPMVLS-PARLGADVVIHSISKFISGGADVIAGAVCGPAKL 276
           ++  + H  G  V+VDN+    VLS P  LGAD+V+HS +KFI+G +DV+AG +    + 
Sbjct: 238 KISEMAHAQGALVLVDNSIMSPVLSRPLELGADIVMHSATKFIAGHSDVMAGVLAVKGEK 297

Query: 277 INSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLSLRM---KEHCRRAAEFAERMKKAGL 336
           +   +   Q S    G  +     +     I  ++LR+   +E+ R+ A +     +   
Sbjct: 298 LAKEVYFLQNS---EGSGLAPFDCWLCLRGIKTMALRIEKQQENARKIAMYLSSHPRV-K 357

Query: 337 KVIYPGLPDHPQHKLMKRLANSEYGFGGMLCLDMGTEERANRLMSVLQNNTQFGFMAVSL 396
           KV Y GLPDHP H L    A    G G +     G+   +  L+      T++  +AVS 
Sbjct: 358 KVYYAGLPDHPGHHLHFSQAK---GAGSVFSFITGSVALSKHLV----ETTKYFSIAVSF 417

Query: 397 GYYETLMSCSGSSTSSEMNREEKQLAGISPGLIRMSIG 429
           G  ++L+S     + + +  E ++  G++  L+R+S G
Sbjct: 418 GSVKSLISMPCFMSHASIPAEVREARGLTEDLVRISAG 429

[Close]

BLAST of MC10g_new0352 vs. TAIR 10
Match: AT3G57050.1 (cystathionine beta-lyase )

HSP 1 Score: 139.0 bits (349), Expect = 9.2e-33
Identity = 113/387 (29.20%), Postives = 197/387 (50.90%), Query Frame = 0


Query: 46  EDDPAAAIANARHEFGEHGGVNMSIEASATFTVMEPETMRRMFSGELGPDRDFFIYSRHF 105
           E   +  + N  ++F     ++  +  +ATF   +P  +      E GP    + Y+R  
Sbjct: 84  EASVSTLLVNLDNKFDPFDAMSTPLYQTATF--KQPSAI------ENGP----YDYTRSG 143

Query: 106 NPTVLNLSRQMAAMEGTEAAYCTSSGMSAIAAVLLQLLASGDHIVASRTLYGGTHALLSH 165
           NPT   L   +A ++  + A+C +SGM+A++AV   L+ +G+ IVA   +YGG+  LLS 
Sbjct: 144 NPTRDALESLLAKLDKADRAFCFTSGMAALSAV-THLIKNGEEIVAGDDVYGGSDRLLSQ 203

Query: 166 FLPRTSGITTSFVDVGNVEMVRAGIVEGKTKVLYFEAVSNPTLAVADVPELCRIGHDHGL 225
            +PR SG+    V+   ++ V A I   +TK+++ E+ +NP   ++D+ ++  + H  G 
Sbjct: 204 VVPR-SGVVVKRVNTTKLDEVAAAI-GPQTKLVWLESPTNPRQQISDIRKISEMAHAQGA 263

Query: 226 TVVVDNTFAPMVLS-PARLGADVVIHSISKFISGGADVIAGAVCGPAKLINSMMDLRQGS 285
            V+VDN+    VLS P  LGAD+V+HS +KFI+G +DV+AG +    + +   +   Q S
Sbjct: 264 LVLVDNSIMSPVLSRPLELGADIVMHSATKFIAGHSDVMAGVLAVKGEKLAKEVYFLQNS 323

Query: 286 LMLLGPTMNAKVAFELSERIPHLSLRM---KEHCRRAAEFAERMKKAGLKVIYPGLPDHP 345
               G  +     +     I  ++LR+   +E+ R+ A +     +   KV Y GLPDHP
Sbjct: 324 ---EGSGLAPFDCWLCLRGIKTMALRIEKQQENARKIAMYLSSHPRV-KKVYYAGLPDHP 383

Query: 346 QHKLMKRLANSEYGFGGMLCLDMGTEERANRLMSVLQNNTQFGFMAVSLGYYETLMSCSG 405
            H L    A    G G +     G+   +  L+      T++  +AVS G  ++L+S   
Sbjct: 384 GHHLHFSQAK---GAGSVFSFITGSVALSKHLV----ETTKYFSIAVSFGSVKSLISMPC 443

Query: 406 SSTSSEMNREEKQLAGISPGLIRMSIG 429
             + + +  E ++  G++  L+R+S G
Sbjct: 444 FMSHASIPAEVREARGLTEDLVRISAG 444

The following BLAST results are available for this feature:

BLAST of MC10g_new0352 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SGU9	1.2e-183	71.81	Methionine gamma-lyase OS=Arabidopsis thaliana OX=3702 GN=MGL PE=1 SV=1	[more]
Q8L0X4	5.4e-54	35.99	L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. polymorphum OX=76857 ...	[more]
Q8RDT4	6.6e-52	34.96	L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. nucleatum (strain ATC...	[more]
Q7MX71	3.3e-51	36.90	L-methionine gamma-lyase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83)...	[more]
P13254	9.9e-48	36.46	L-methionine gamma-lyase OS=Pseudomonas putida OX=303 GN=mdeA PE=1 SV=2	[more]

BLAST of MC10g_new0352 vs. NCBI nr


Match Name	E-value	Identity	Description
NP_001315378.1	3.54e-272	82.49	methionine gamma-lyase-like [Cucumis melo] >AGF70153.1 methionine gamma-lyase [C...	[more]
XP_038876588.1	3.01e-271	83.62	methionine gamma-lyase-like [Benincasa hispida]	[more]
KAA0052969.1	5.85e-271	82.28	methionine gamma-lyase-like [Cucumis melo var. makuwa] >TYK11425.1 methionine ga...	[more]
XP_023553436.1	3.38e-270	85.14	methionine gamma-lyase-like [Cucurbita pepo subsp. pepo]	[more]
KAG7015808.1	1.37e-269	84.91	Methionine gamma-lyase [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of MC10g_new0352 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
M1NFB7	1.71e-272	82.49	Methionine gamma-lyase OS=Cucumis melo OX=3656 GN=MGL PE=2 SV=1	[more]
A0A5A7UHF4	2.83e-271	82.28	Methionine gamma-lyase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3BKU9	2.83e-271	82.28	methionine gamma-lyase-like OS=Cucumis melo OX=3656 GN=LOC103490704 PE=3 SV=1	[more]
A0A0A0L129	6.90e-270	83.63	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G002890 PE=3 SV=1	[more]
A0A6J1EC60	1.90e-269	84.68	methionine gamma-lyase-like OS=Cucurbita moschata OX=3662 GN=LOC111431210 PE=3 S...	[more]

BLAST of MC10g_new0352 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G64660.1	8.8e-185	71.81	methionine gamma-lyase	[more]
AT3G01120.1	7.5e-43	33.13	Pyridoxal phosphate (PLP)-dependent transferases superfamily protein	[more]
AT1G33320.1	3.8e-34	28.87	Pyridoxal phosphate (PLP)-dependent transferases superfamily protein	[more]
AT3G57050.2	2.4e-33	28.89	cystathionine beta-lyase	[more]
AT3G57050.1	9.2e-33	29.20	cystathionine beta-lyase	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000277	Cys/Met metabolism, pyridoxal phosphate-dependent enzyme	PIRSF	PIRSF001434	CGS	coord: 34..448 e-value: 3.1E-106 score: 353.4
IPR000277	Cys/Met metabolism, pyridoxal phosphate-dependent enzyme	PFAM	PF01053	Cys_Met_Meta_PP	coord: 58..428 e-value: 6.2E-85 score: 285.0
IPR000277	Cys/Met metabolism, pyridoxal phosphate-dependent enzyme	PANTHER	PTHR11808	TRANS-SULFURATION ENZYME FAMILY MEMBER	coord: 44..448
IPR000277	Cys/Met metabolism, pyridoxal phosphate-dependent enzyme	CDD	cd00614	CGS_like	coord: 64..445 e-value: 1.26586E-134 score: 390.41
IPR015421	Pyridoxal phosphate-dependent transferase, major domain	GENE3D	3.40.640.10		coord: 50..304 e-value: 9.7E-68 score: 230.2
IPR015422	Pyridoxal phosphate-dependent transferase, small domain	GENE3D	3.90.1150.10	Aspartate Aminotransferase, domain 1	coord: 308..447 e-value: 1.6E-28 score: 101.7
None	No IPR available	PIRSR	PIRSR001434-2	PIRSR001434-2	coord: 102..408 e-value: 1.7E-82 score: 274.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..33
None	No IPR available	PANTHER	PTHR11808:SF76	METHIONINE GAMMA-LYASE-LIKE	coord: 44..448
IPR015424	Pyridoxal phosphate-dependent transferase	SUPERFAMILY	53383	PLP-dependent transferases	coord: 61..443

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g_new0352.1	MC10g_new0352.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019343	cysteine biosynthetic process via cystathionine
biological_process	GO:0019346	transsulfuration
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004123	cystathionine gamma-lyase activity
molecular_function	GO:0030170	pyridoxal phosphate binding
molecular_function	GO:0003824	catalytic activity