MC10g_new0324 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g_new0324
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	(-)-germacrene D synthase-like
Location	MC10: 15649493 .. 15653091 (+)
RNA-Seq Expression	MC10g_new0324
Synteny	MC10g_new0324

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGATGCATGGAGAGGATGTTCTTGAACGGGCCCTCGCTTTTAGCACCGCAAAATTAAAAGCAGCCATTAGTAATGGAAACCATGGAGGGAGCTGTGGGTTTGGAGATCAAGTGAGGCATGCATTGAAGTGGCCAGTCTTCAAAGCCATGCCTAGGCTTGCAGCAAGAAATTACAAATCTTTCTATGCACAAGATCCATTGCATCACCCTACTTTACTTTCCTTTGCCAAGCTGGATTATAACATCTTGCAAAAGTTTTACCAAAGAGAACTTCATGAATTCTCAAGGTCAGAAATATCCATCTCTTTAGAGTTCATAAGTAGTACTTTTTTTTTTTTTTTGCTCCTGTTTTCAAACTATATGCATATATTTTGTGTTTTTAGAGAGATTTGATTTGGGTTTGTGATAATTTCTAAAATAGTATGACATTACTGTTAATATTGGCATAGCCTATTTGGTTAAGACATTATTATCCTCTTTAAATGTTAAAGGTTTCGAATCCCCGCTCTATGTTTAACTTTAAGAAAATGATCATATTATTGAGTATGCACTTGAAGTCAGATTGTTAAAAGGTGGAAACATCCCAAAAAGAAATTAGTGTTGATTTCAAGGAATGCTACCACTTTTTCATAAGACCTATCTTTTTGAGGTTTGAAGTCTACGACTTTGCTTTTGGTTCAATCAAAAAGATCTCATAGTAATATAGATATTTTGTTTACTTTGTTTTTCTTTTAGGAGCTCACTGTATAGAGTTGAAAATTCAAACATATTAATGTTTCATCTCAAAACCAATTGGCAACAAGATGAGTAATTCACTTATATTAAAAATCTTTGTTCGGTCCCTTATTTTTTCAATGTGGAATCCGTAATATGTCCCCTCAAGATGGTTACCTCTTTTGAGTTCACCAATCTCTTTGGTCAGATCCCATTTTTTGATCGGACACTCATTTGACTTTTGGGCTCTAATATTGTATCAATAGTCATGAGATTTCATCTCAAAATCAATTGGCAACGGAAAAAAGTAGCCAATTTATATTATAAACTCTTGTTAGGTCTCTTATATTTTAATTAAGACGGGCATATTCTTAGAATTTGTTTCAAGCAACTCAATATTATTATCTACGTATACATATATAGGTGGTGGAAAGAGTTGAAGCTGGTGGAGAAGCTGGGTTTTGCGAGAGATAGATGCGTGGAATGTTATATTTGGGCATTGGGACTATTCTTTGAGCCAAAATAATCTCTTGCTCGAACAAACATGACAAAACTTGTGGCGATGACTTCAATTTTGGATGATATATACTATGTTTATGGTACATTCGAAGAACCCCAAGTTTTCACTGGAACAATTCAAAGGTATATACGTTACTCGGATAAAAATACCAACTTTATGATTTTCAATTATGTTCCTGAATTTTGGATTATGCTTATACTTAGTCTAACTCTCCTTAACGTCACCAAAATGCTAAGGTGACATGTTTATTAATTTAGATTTTGAATTGTGTACATATGATCATATCTCGTGGCGGGTCTACATTGGGATCAGTGGCACGTGCCCTTGAATTTGATTAAATAATATATATTATGTTACAAATAGATAATATGGTCTCTAGTATGGTTGGTGTGTAGTTGCCCCTACATTGCCTTTCCATGCCTCTCAACTTGTGTTCAAATCTTTTCAATTCTGCGCAAGTCATTTTTAACAAATATTCTAGTATTATATTAAACAAACTAATATCAGTGATATTATAGTTTAATTTTCAATATTATTTAATTGGATGTTTGTAACAATACATTTGATTATTTAGGAAAAAAATCAATTTTTTTTCCTAAACTAGATAAGTTGTATCAAGATTCACCGTAATCATTCAATTTCAATAAATTCGTCGGCCACTAGATCGATGAAAAATCTCTCCAAAATTTGATCGTTTGATGAGTGATTCACCAACCACTAGATCCCAAACCTAAAAAATTAGGTAAGTATTGCAATTTTTATCATTTATTCAACCTTTCCTCCGCTAGTTGAACTATTAATTTAAACCAGAACCATTGGAAAAAAAAACTAAAAAGAACCACTAATTTAACAAAAATCAAGCTTTTTACAAATTATGCACAAAAAGCAATCATTAAATAACTACAAATTTCAAAAAAGAAATCTAACTTTAGTATGGTTTTTATTTGAATTCTATCAATTTCCTATCAAATATATGTTTGGTAATTCATTTAATACATATAAGAATACTATTTCAACAAATTTTCTAATGAAAATTTACGAGAGGGTAAAAATTGCAACGTTTTTTAAAGTTTAAGGTTAATATTGCAACATTTATCCAAATTTACGGTGTGATTTGATTAATTGATGCACATTGCTAAGTTCGGGGTTAAAATTGATCTTCCCCGTTATGTTTTTTTTGTAGTTGTGTGCCTCCTCTAACATCAAATCCTCCTAATCACATATATAAGCATTTTGTTAATCCAAAGTACATAAATGTATTAAACTTTTTGTTATCTTTCCACTTTTTAGGTGGGATGTTAATTGTAGTGGCGGATCCAGAATTTTGTCGAGGGGGCGTGAAGAAAAAATGTACTAATTTAGATAATAAAAGATACTACTTTCTAACTTACCTTATATTATTCTTTATTATCTCATAATAAAAGATATTCCTCTCTCCCCCTCTTTACTTTCTAACTTCATTGCTATGATTTAACTAACTCTGCTAGATACTACTTTCTTGAAGCTAATTGGTATCATGATGGCTATACACCAACAATGGATGAATACTTGAAAGTTGCAAGAGTTTCTTCTTGGGTCTATTTGCTCTCTCGCTTCATTTCTCAGCGTGGGAGATGATGTTTCGATGGAGGTATTTGAATGGATCGATAGTGATTCGAAATTGCTTATCGCATCTGAAGTCATTAGTAGGATCATGAACGATATTACTTCCCACAAGGTAATTAAACTATTAAGATTATAATCTTTGATCTTAACATGTGAATTTTTTTAAACTTTAGTAGTAATTTACTATGATAAAAACATATTGGGGTAGGAATTCGAACCTTTGACCTACTGGTTGCAGATATACGTCTTAACCAGTTAGATTATCATCAAGTTGATAATTTATTGGGGTATTATGGTAAGACATGAACCCATCAACAAAACATGTCCAAATACAATTCATAACAAACAGCCATCATCATCACCATCAAAATGACATAAATTGATGCGAATAAAAATAGTTTCACCCATTAACAAGAATTTACCTAGTTTTACAATCGAGCATTAACAACTAATTAAGGCATTTATATTTTTCTTTGAGGTCAAAGATCCAAATCTCCAAAACACATTTGTTGTATTTAAAGTAAAAAAAAAATTAAAAAGAAAAGTAGATGACTTATTTAAGTTTTTGTATAATTTAATGAGACTAAAAAAATGATGACTTATTTTAAATTTTTGGATCGATATATAGTTTGAACAAGAGAGGGGTCATGTTGCTTCAGCCAACGAATGCTACATGACACAATATGGCGTATCAGAAGAAGAGGCTATAGAAGAATTAGAGAAGCAAGTTACAAAATGGCTGGAAGGAAATCAATGA

mRNA sequence

ATGCAGATGCATGGAGAGGATGTTCTTGAACGGGCCCTCGCTTTTAGCACCGCAAAATTAAAAGCAGCCATTAGTAATGGAAACCATGGAGGGAGCTGTGGGTTTGGAGATCAAGTGAGGCATGCATTGAAGTGGCCAGTCTTCAAAGCCATGCCTAGGCTTGCAGCAAGAAATTACAAATCTTTCTATGCACAAGATCCATTGCATCACCCTACTTTACTTTCCTTTGCCAAGCTGGATTATAACATCTTGCAAAAGTTTTACCAAAGAGAACTTCATGAATTCTCAAGCGTGGGAGATGATGTTTCGATGGAGGTATTTGAATGGATCGATAGTGATTCGAAATTGCTTATCGCATCTGAAGTCATTAGTAGGATCATGAACGATATTACTTCCCACAAGTTTGAACAAGAGAGGGGTCATGTTGCTTCAGCCAACGAATGCTACATGACACAATATGGCGTATCAGAAGAAGAGGCTATAGAAGAATTAGAGAAGCAAGTTACAAAATGGCTGGAAGGAAATCAATGA

Coding sequence (CDS)

ATGCAGATGCATGGAGAGGATGTTCTTGAACGGGCCCTCGCTTTTAGCACCGCAAAATTAAAAGCAGCCATTAGTAATGGAAACCATGGAGGGAGCTGTGGGTTTGGAGATCAAGTGAGGCATGCATTGAAGTGGCCAGTCTTCAAAGCCATGCCTAGGCTTGCAGCAAGAAATTACAAATCTTTCTATGCACAAGATCCATTGCATCACCCTACTTTACTTTCCTTTGCCAAGCTGGATTATAACATCTTGCAAAAGTTTTACCAAAGAGAACTTCATGAATTCTCAAGCGTGGGAGATGATGTTTCGATGGAGGTATTTGAATGGATCGATAGTGATTCGAAATTGCTTATCGCATCTGAAGTCATTAGTAGGATCATGAACGATATTACTTCCCACAAGTTTGAACAAGAGAGGGGTCATGTTGCTTCAGCCAACGAATGCTACATGACACAATATGGCGTATCAGAAGAAGAGGCTATAGAAGAATTAGAGAAGCAAGTTACAAAATGGCTGGAAGGAAATCAATGA

Protein sequence

MQMHGEDVLERALAFSTAKLKAAISNGNHGGSCGFGDQVRHALKWPVFKAMPRLAARNYKSFYAQDPLHHPTLLSFAKLDYNILQKFYQRELHEFSSVGDDVSMEVFEWIDSDSKLLIASEVISRIMNDITSHKFEQERGHVASANECYMTQYGVSEEEAIEELEKQVTKWLEGNQ

Homology

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BLAST of MC10g_new0324 vs. ExPASy Swiss-Prot
Match: Q6Q3H2 (Valencene synthase OS=Vitis vinifera OX=29760 GN=ValCS PE=1 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 2.0e-20
Identity = 88/343 (25.66%), Postives = 120/343 (34.99%), Query Frame = 0


Query: 1   MQMHGEDVLERALAFSTAKLKAAI-SNGNHGGSCGFGDQVRHALKWPVFKAMPRLAARNY 60
           +++HGED+L +ALAF+T  LKA + S G H       +QV HAL  P+ K + RL AR Y
Sbjct: 166 LRVHGEDILAKALAFTTTHLKAMVESLGYH-----LAEQVAHALNRPIRKGLERLEARWY 225

Query: 61  KSFYAQDPLHHPTLLSFAKLDYNILQKFYQRE---------------------------- 120
            S Y  +  H  TLL  AKLD+N++Q  ++ E                            
Sbjct: 226 ISVYQDEAFHDKTLLELAKLDFNLVQSLHKEELSNLARWWKELDFATKLPFARDRLVEGY 285

Query: 121 ------------------------------------------------------------ 177
                                                                       
Sbjct: 286 FWMHGVYFEPQYLRGRRILTKVIAMTSILDDIHDAYGTPEELKLFIEAIERWDINSINQL 345

[Close]

BLAST of MC10g_new0324 vs. ExPASy Swiss-Prot
Match: Q6Q3H3 ((-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 4.9e-19
Identity = 83/341 (24.34%), Postives = 121/341 (35.48%), Query Frame = 0


Query: 2   QMHGEDVLERALAFSTAKLKAAISNGNHGGSCGFGDQVRHALKWPVFKAMPRLAARNYKS 61
           ++HGED+L+ ALAF+T  L++A  + ++       +QV HALK P+ K +PRL AR+Y S
Sbjct: 167 RVHGEDILDEALAFTTTHLQSATKHSSN----PLAEQVVHALKQPIRKGLPRLEARHYFS 226

Query: 62  FYAQDPLHHPTLLSFAKLDYNILQKFYQRELHEFSS------------------------ 121
            Y  D  H+  LL  AKLD+N+LQK +Q+EL + S+                        
Sbjct: 227 VYQADDSHNKALLKLAKLDFNLLQKLHQKELSDISAWWKDLDFAHKLPFARDRVVECYFW 286

Query: 122 ------------------------------------------------------------ 177
                                                                       
Sbjct: 287 ILGVYFEPQFFFARRILTKVIAMTSIIDDIYDVYGTLEELELFTEAVERWDISAIDQLPE 346

[Close]

BLAST of MC10g_new0324 vs. ExPASy Swiss-Prot
Match: B2KSJ5 ((+)-gamma-cadinene synthase OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 2.4e-18
Identity = 88/343 (25.66%), Postives = 118/343 (34.40%), Query Frame = 0


Query: 1   MQMHGEDVLERALAFSTAKLKAAISNGNHGGSCGFGDQVRHALKWPVFKAMPRLAARNYK 60
           M  HGE +LE AL F+T  L+  I   ++     F  +V +ALK P+ K++PR+ AR Y 
Sbjct: 176 MMGHGEALLEEALEFTTTHLQTYIHRYSNINP-SFASEVSNALKLPIRKSVPRIKAREYL 235

Query: 61  SFYAQDPLHHPTLLSFAKLDYNILQKFYQRELHE-------------------------- 120
             Y Q P H+ TLL F+KLD+NILQK +Q+EL E                          
Sbjct: 236 EIYQQHPSHNETLLEFSKLDFNILQKLHQKELSEICRWWKDLDVPTKFPFARDRIVECYF 295

Query: 121 ------------------------------------------------------------ 177
                                                                       
Sbjct: 296 WTLGAYFEPQYSVGRKMLTKVIAIASILDDIYDAYGTFEELQVLTPAIQRWDRSMVHTLP 355

[Close]

BLAST of MC10g_new0324 vs. ExPASy Swiss-Prot
Match: W0FFD7 ((-)-drimenol synthase OS=Persicaria hydropiper OX=46901 PE=1 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 7.7e-17
Identity = 45/105 (42.86%), Postives = 65/105 (61.90%), Query Frame = 0


Query: 1   MQMHGEDVLERALAFSTAKLKAAISN-GNHGGSCGFGDQVRHALKWPVFKAMPRLAARNY 60
           ++ HG+D L+ AL F+T  LKA ++N  NH        QV HAL  P  K MPRL +R++
Sbjct: 165 VRTHGDDTLDEALVFTTTHLKAVVTNQPNH----PLVPQVTHALMQPYHKGMPRLESRHF 224

Query: 61  KSFYAQDPLHHPTLLSFAKLDYNILQKFYQRELHEFSSVGDDVSM 105
            +FY +DP H  TLL F KLD+N++Q  +++EL + S    D+ M
Sbjct: 225 IAFYEKDPYHDKTLLKFGKLDFNLVQALHKKELKDLSRWWKDLDM 265

HSP 2 Score: 70.1 bits (170), Expect = 2.9e-11
Identity = 38/83 (45.78%), Postives = 53/83 (63.86%), Query Frame = 0


Query: 95  FSSVGDDVSMEVFEWIDSDSKLLIASEVISRIMNDITSHKFEQERGHVASANECYMTQYG 154
           F  +GD  + E FEW   + K++ A+ +I R+M+DI SH FEQ R HV SA ECY+ Q+G
Sbjct: 423 FLGMGDVATKEAFEWARGNPKVVRAAGIIGRLMDDIGSHHFEQGRDHVPSAVECYIRQHG 482

Query: 155 VSEEEAIEELEKQV-TKWLEGNQ 177
           V E  A  EL K+V + W + N+
Sbjct: 483 VDEVTAQRELGKRVESSWKDINE 505

[Close]

BLAST of MC10g_new0324 vs. ExPASy Swiss-Prot
Match: B5A435 (Sesquiterpene synthase OS=Santalum album OX=35974 PE=1 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 3.8e-16
Identity = 43/83 (51.81%), Postives = 59/83 (71.08%), Query Frame = 0


Query: 95  FSSVGDDVSMEVFEWIDSDSKLLIASEVISRIMNDITSHKFEQERGHVASANECYMTQYG 154
           +  +G+  S E F+W+ S   ++ ASE + R+M+D+ SHKFEQERGHVAS  ECYM QYG
Sbjct: 423 YLGMGEIASKEAFDWLFSHPPVIEASESVCRLMDDMRSHKFEQERGHVASGIECYMKQYG 482

Query: 155 VSEEEAIEELEKQVTK-WLEGNQ 177
           V+EEEA +E  KQ+ K W + N+
Sbjct: 483 VTEEEAHDEFRKQLVKAWKDINE 505

HSP 2 Score: 68.6 bits (166), Expect = 8.3e-11
Identity = 34/96 (35.42%), Postives = 59/96 (61.46%), Query Frame = 0


Query: 1   MQMHGEDVLERALAFSTAKLKAAISNGNHGGSCGFGDQVRHALKWPVFKAMPRLAARNYK 60
           + +HGE++L+ AL F+  +L++ +++ N+       +QVRHAL  P+ K +PRL A  + 
Sbjct: 168 LSVHGEEMLDDALEFAKTRLESIVNHLNY----PLAEQVRHALYRPLRKGLPRLEAVYFF 227

Query: 61  SFYAQDPLHHPTLLSFAKLDYNILQKFYQRELHEFS 97
             Y     H+  LL  AKLD+N+LQ  +++EL + +
Sbjct: 228 RIYEAYDSHNKALLKLAKLDFNLLQSLHKKELSDMA 259

[Close]

BLAST of MC10g_new0324 vs. NCBI nr
Match: XP_022154020.1 ((+)-gamma-cadinene synthase-like [Momordica charantia])

HSP 1 Score: 219 bits (558), Expect = 7.93e-66
Identity = 131/267 (49.06%), Postives = 143/267 (53.56%), Query Frame = 0


Query: 1   MQMHGEDVLERALAFSTAKLKAAISNGNHGGSCGFGDQVRHALKWPVFKAMPRLAARNYK 60
           +++HGEDVLERAL+FST  LKAAISNG+H GSCG G QVRHALKWPVFKAMPRLAAR+Y 
Sbjct: 170 VRIHGEDVLERALSFSTTNLKAAISNGSHEGSCGIGGQVRHALKWPVFKAMPRLAARSYI 229

Query: 61  SFYAQDPLHHPTLLSFAKLDYNILQKFYQRELHEFS------------------------ 120
           SFY QDPLHHPTLL  AKLDYNILQK YQ+ELHE S                        
Sbjct: 230 SFYEQDPLHHPTLLYLAKLDYNILQKLYQKELHEISRWDDVNCIEGLPKYMKVYYEALMD 289

Query: 121 ------------------------------------------------------------ 173
                                                                       
Sbjct: 290 TYRGIEHDISKDDISYALHYAKEAMKRQARNYFFEAKWYHDGYTPTIEEYLRVGRVTACY 349

[Close]

BLAST of MC10g_new0324 vs. NCBI nr
Match: XP_022154019.1 ((-)-germacrene D synthase-like [Momordica charantia])

HSP 1 Score: 209 bits (533), Expect = 1.85e-61
Identity = 140/339 (41.30%), Postives = 149/339 (43.95%), Query Frame = 0


Query: 1   MQMHGEDVLERALAFSTAKLKAAISNGNHGGSCGFGDQVRHALKWPVFKAMPRLAARNYK 60
           ++MHGEDVLERALAFST  LKAAISNG+H GS GFG QVRHALKWPVFKAMPRLAARNY 
Sbjct: 170 VRMHGEDVLERALAFSTTNLKAAISNGSHEGSWGFGAQVRHALKWPVFKAMPRLAARNYI 229

Query: 61  SFYAQDPLHHPTLLSFAKLDYNILQKFYQRELHE-------------------------- 120
           SFY QDPLHHPTLLSFAKLDYN LQK YQRELHE                          
Sbjct: 230 SFYEQDPLHHPTLLSFAKLDYNTLQKLYQRELHEITRWWKDLKLVENLCFARDRCVECYT 289

Query: 121 ------------------------------------------------------------ 173
                                                                       
Sbjct: 290 WALGTYFEPKYSLGRIMLSKIVVITSVLDDIFDLHGTFEELQLFIHAIDRWDVNCIERLP 349

[Close]

BLAST of MC10g_new0324 vs. NCBI nr
Match: XP_038905736.1 ((-)-germacrene D synthase-like [Benincasa hispida])

HSP 1 Score: 184 bits (466), Expect = 1.26e-51
Identity = 126/332 (37.95%), Postives = 141/332 (42.47%), Query Frame = 0


Query: 1   MQMHGEDVLERALAFSTAKLKAAISNGNHGGSCGFGDQVRHALKWPVFKAMPRLAARNYK 60
           MQMHGEDVLERALAF+TA L+A I+N N       G++ R+ALKWP+FKAMPRLAA+NY 
Sbjct: 176 MQMHGEDVLERALAFTTANLRAIINNSN----ASHGEEGRYALKWPLFKAMPRLAAKNYI 235

Query: 61  SFYAQDPLHHPTLLSFAKLDYNILQKFYQRELHEFS------------------------ 120
           SFY +DPLH+PTLLSFAKLDYN LQK YQ+ELHE S                        
Sbjct: 236 SFYEKDPLHNPTLLSFAKLDYNCLQKLYQKELHEISRWWKDLKLVEKLCFARDRCVECYI 295

Query: 121 ------------------------------------------------------------ 168
                                                                       
Sbjct: 296 WALGLCFEPKYSLARKITSKIAMIASILDDIYDAYGTFQELQQFTHAIQRWDVNCIEGLP 355

[Close]

BLAST of MC10g_new0324 vs. NCBI nr
Match: XP_022154012.1 ((-)-germacrene D synthase-like [Momordica charantia])

HSP 1 Score: 164 bits (415), Expect = 1.73e-45
Identity = 77/96 (80.21%), Postives = 85/96 (88.54%), Query Frame = 0


Query: 1   MQMHGEDVLERALAFSTAKLKAAISNGNHGGSCGFGDQVRHALKWPVFKAMPRLAARNYK 60
           M+MHGEDVLERAL FST  L+AAI+NG+H GSCGFG QVRHALKWPVFKAMPRLAAR+Y 
Sbjct: 170 MRMHGEDVLERALTFSTINLEAAINNGSHEGSCGFGAQVRHALKWPVFKAMPRLAARSYI 229

Query: 61  SFYAQDPLHHPTLLSFAKLDYNILQKFYQRELHEFS 96
           SFY +DPLHHP LLS AKLD+NILQK YQ+ELHE S
Sbjct: 230 SFYEEDPLHHPALLSMAKLDFNILQKLYQKELHEIS 265

[Close]

BLAST of MC10g_new0324 vs. NCBI nr
Match: XP_022144020.1 ((-)-germacrene D synthase-like [Momordica charantia])

HSP 1 Score: 163 bits (413), Expect = 3.24e-43
Identity = 114/333 (34.23%), Postives = 132/333 (39.64%), Query Frame = 0


Query: 1   MQMHGEDVLERALAFSTAKLKAAISNGNHGGSCGFGDQVRHALKWPVFKAMPRLAARNYK 60
           M+MHGED+LER   FST  LKAAI++       GF  QVRHALKWP+ KA+PR   R+Y 
Sbjct: 351 MRMHGEDILERGFGFSTTSLKAAIASHVED---GFATQVRHALKWPLMKAIPRFGIRHYI 410

Query: 61  SFYAQDPLHHPTLLSFAKLDYNILQKFYQRELHEFS------------------------ 120
           SFY  DPLHHP LLSFAKLD+ +LQ+ Y +EL E S                        
Sbjct: 411 SFYETDPLHHPVLLSFAKLDFGVLQRLYNKELREISRWWKDLKLVEKLSFARDRCVECYI 470

Query: 121 ------------------------------------------------------------ 169
                                                                       
Sbjct: 471 WAMAICFEPKYSLARIITSKIIMMQSILDDIYDAYGTFEELQLFTRAIERWNVKCIDELP 530

[Close]

BLAST of MC10g_new0324 vs. ExPASy TrEMBL
Match: A0A6J1DIG3 ((+)-gamma-cadinene synthase-like OS=Momordica charantia OX=3673 GN=LOC111021378 PE=4 SV=1)

HSP 1 Score: 219 bits (558), Expect = 3.84e-66
Identity = 131/267 (49.06%), Postives = 143/267 (53.56%), Query Frame = 0


Query: 1   MQMHGEDVLERALAFSTAKLKAAISNGNHGGSCGFGDQVRHALKWPVFKAMPRLAARNYK 60
           +++HGEDVLERAL+FST  LKAAISNG+H GSCG G QVRHALKWPVFKAMPRLAAR+Y 
Sbjct: 170 VRIHGEDVLERALSFSTTNLKAAISNGSHEGSCGIGGQVRHALKWPVFKAMPRLAARSYI 229

Query: 61  SFYAQDPLHHPTLLSFAKLDYNILQKFYQRELHEFS------------------------ 120
           SFY QDPLHHPTLL  AKLDYNILQK YQ+ELHE S                        
Sbjct: 230 SFYEQDPLHHPTLLYLAKLDYNILQKLYQKELHEISRWDDVNCIEGLPKYMKVYYEALMD 289

Query: 121 ------------------------------------------------------------ 173
                                                                       
Sbjct: 290 TYRGIEHDISKDDISYALHYAKEAMKRQARNYFFEAKWYHDGYTPTIEEYLRVGRVTACY 349

[Close]

BLAST of MC10g_new0324 vs. ExPASy TrEMBL
Match: A0A6J1DKT6 ((-)-germacrene D synthase-like OS=Momordica charantia OX=3673 GN=LOC111021377 PE=4 SV=1)

HSP 1 Score: 209 bits (533), Expect = 8.95e-62
Identity = 140/339 (41.30%), Postives = 149/339 (43.95%), Query Frame = 0


Query: 1   MQMHGEDVLERALAFSTAKLKAAISNGNHGGSCGFGDQVRHALKWPVFKAMPRLAARNYK 60
           ++MHGEDVLERALAFST  LKAAISNG+H GS GFG QVRHALKWPVFKAMPRLAARNY 
Sbjct: 170 VRMHGEDVLERALAFSTTNLKAAISNGSHEGSWGFGAQVRHALKWPVFKAMPRLAARNYI 229

Query: 61  SFYAQDPLHHPTLLSFAKLDYNILQKFYQRELHE-------------------------- 120
           SFY QDPLHHPTLLSFAKLDYN LQK YQRELHE                          
Sbjct: 230 SFYEQDPLHHPTLLSFAKLDYNTLQKLYQRELHEITRWWKDLKLVENLCFARDRCVECYT 289

Query: 121 ------------------------------------------------------------ 173
                                                                       
Sbjct: 290 WALGTYFEPKYSLGRIMLSKIVVITSVLDDIFDLHGTFEELQLFIHAIDRWDVNCIERLP 349

[Close]

BLAST of MC10g_new0324 vs. ExPASy TrEMBL
Match: A0A6J1DKI4 ((-)-germacrene D synthase-like OS=Momordica charantia OX=3673 GN=LOC111021373 PE=4 SV=1)

HSP 1 Score: 164 bits (415), Expect = 8.40e-46
Identity = 77/96 (80.21%), Postives = 85/96 (88.54%), Query Frame = 0


Query: 1   MQMHGEDVLERALAFSTAKLKAAISNGNHGGSCGFGDQVRHALKWPVFKAMPRLAARNYK 60
           M+MHGEDVLERAL FST  L+AAI+NG+H GSCGFG QVRHALKWPVFKAMPRLAAR+Y 
Sbjct: 170 MRMHGEDVLERALTFSTINLEAAINNGSHEGSCGFGAQVRHALKWPVFKAMPRLAARSYI 229

Query: 61  SFYAQDPLHHPTLLSFAKLDYNILQKFYQRELHEFS 96
           SFY +DPLHHP LLS AKLD+NILQK YQ+ELHE S
Sbjct: 230 SFYEEDPLHHPALLSMAKLDFNILQKLYQKELHEIS 265

[Close]

BLAST of MC10g_new0324 vs. ExPASy TrEMBL
Match: A0A6J1CQG9 ((-)-germacrene D synthase-like OS=Momordica charantia OX=3673 GN=LOC111013811 PE=4 SV=1)

HSP 1 Score: 163 bits (413), Expect = 1.57e-43
Identity = 114/333 (34.23%), Postives = 132/333 (39.64%), Query Frame = 0


Query: 1   MQMHGEDVLERALAFSTAKLKAAISNGNHGGSCGFGDQVRHALKWPVFKAMPRLAARNYK 60
           M+MHGED+LER   FST  LKAAI++       GF  QVRHALKWP+ KA+PR   R+Y 
Sbjct: 351 MRMHGEDILERGFGFSTTSLKAAIASHVED---GFATQVRHALKWPLMKAIPRFGIRHYI 410

Query: 61  SFYAQDPLHHPTLLSFAKLDYNILQKFYQRELHEFS------------------------ 120
           SFY  DPLHHP LLSFAKLD+ +LQ+ Y +EL E S                        
Sbjct: 411 SFYETDPLHHPVLLSFAKLDFGVLQRLYNKELREISRWWKDLKLVEKLSFARDRCVECYI 470

Query: 121 ------------------------------------------------------------ 169
                                                                       
Sbjct: 471 WAMAICFEPKYSLARIITSKIIMMQSILDDIYDAYGTFEELQLFTRAIERWNVKCIDELP 530

[Close]

BLAST of MC10g_new0324 vs. ExPASy TrEMBL
Match: A0A5D3CJ95 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1017G00220 PE=4 SV=1)

HSP 1 Score: 162 bits (409), Expect = 8.06e-43
Identity = 115/315 (36.51%), Postives = 133/315 (42.22%), Query Frame = 0


Query: 3   MHGEDVLERALAFSTAKLKAAI--SNGNHGGSCGFGDQVRHALKWPVFKAMPRLAARNYK 62
           MHGE++LERA AF+T  L+AAI  SNG+H        ++ +ALKWP FKAMPRLA+RNY 
Sbjct: 1   MHGEEILERAFAFTTTNLRAAINNSNGSHEDR-----EISYALKWPFFKAMPRLASRNYI 60

Query: 63  SFYAQDPLHHPTLLSFAKLDYNILQKFYQRELHEFS------------------------ 122
           S Y +DPLH+PTLL FAKLDYN LQK YQ+ELHE S                        
Sbjct: 61  SSYEEDPLHNPTLLCFAKLDYNCLQKLYQKELHEISRWWKELKLMEKLSFARDRCVECYI 120

Query: 123 -------------------------SVGDDV----------------------------- 169
                                    S+ DD+                             
Sbjct: 121 WALGIYFEPEYSLGRTVVTKVVVLTSIMDDIYDLHATFEELQLLTHAIESSSTYELFYDP 180

[Close]

BLAST of MC10g_new0324 vs. TAIR 10
Match: AT5G23960.1 (terpene synthase 21 )

HSP 1 Score: 72.4 bits (176), Expect = 4.1e-13
Identity = 36/83 (43.37%), Postives = 56/83 (67.47%), Query Frame = 0


Query: 95  FSSVGDDVSMEVFEWIDSDSKLLIASEVISRIMNDITSHKFEQERGHVASANECYMTQYG 154
           F  + D   ++ FEW+ S  K+ +ASE+ISR  +DI+S++FE +R HVA+  +CYM Q+G
Sbjct: 412 FVRMTDVAKLDAFEWLSSHPKIRVASEIISRFTDDISSYEFEHKREHVATGIDCYMQQFG 471

Query: 155 VSEEEAIEELEKQVT-KWLEGNQ 177
           VS+E A+E +   V+  W + NQ
Sbjct: 472 VSKERAVEVMGNIVSDAWKDLNQ 494

HSP 2 Score: 69.3 bits (168), Expect = 3.5e-12
Identity = 35/89 (39.33%), Postives = 54/89 (60.67%), Query Frame = 0


Query: 4   HGEDVLERALAFSTAKLKAAISNGNHGGSCGFGDQVRHALKWPVFKAMPRLAARNYKSFY 63
           HGED+++ ALAFS + L+   S      S     ++++ALK P  K + R+  R Y S+Y
Sbjct: 158 HGEDIIDEALAFSRSHLEEISSR----SSPHLAIRIKNALKHPYHKGISRIETRQYISYY 217

Query: 64  AQDPLHHPTLLSFAKLDYNILQKFYQREL 93
            ++    PTLL FAK+D+N+LQ  ++ EL
Sbjct: 218 EEEESCDPTLLEFAKIDFNLLQILHREEL 242

[Close]

BLAST of MC10g_new0324 vs. TAIR 10
Match: AT5G23960.2 (terpene synthase 21 )

HSP 1 Score: 72.4 bits (176), Expect = 4.1e-13
Identity = 36/83 (43.37%), Postives = 56/83 (67.47%), Query Frame = 0


Query: 95  FSSVGDDVSMEVFEWIDSDSKLLIASEVISRIMNDITSHKFEQERGHVASANECYMTQYG 154
           F  + D   ++ FEW+ S  K+ +ASE+ISR  +DI+S++FE +R HVA+  +CYM Q+G
Sbjct: 410 FVRMTDVAKLDAFEWLSSHPKIRVASEIISRFTDDISSYEFEHKREHVATGIDCYMQQFG 469

Query: 155 VSEEEAIEELEKQVT-KWLEGNQ 177
           VS+E A+E +   V+  W + NQ
Sbjct: 470 VSKERAVEVMGNIVSDAWKDLNQ 492

HSP 2 Score: 69.3 bits (168), Expect = 3.5e-12
Identity = 35/89 (39.33%), Postives = 54/89 (60.67%), Query Frame = 0


Query: 4   HGEDVLERALAFSTAKLKAAISNGNHGGSCGFGDQVRHALKWPVFKAMPRLAARNYKSFY 63
           HGED+++ ALAFS + L+   S      S     ++++ALK P  K + R+  R Y S+Y
Sbjct: 158 HGEDIIDEALAFSRSHLEEISSR----SSPHLAIRIKNALKHPYHKGISRIETRQYISYY 217

Query: 64  AQDPLHHPTLLSFAKLDYNILQKFYQREL 93
            ++    PTLL FAK+D+N+LQ  ++ EL
Sbjct: 218 EEEESCDPTLLEFAKIDFNLLQILHREEL 242

[Close]

BLAST of MC10g_new0324 vs. TAIR 10
Match: AT4G20200.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 63.2 bits (152), Expect = 2.5e-10
Identity = 32/74 (43.24%), Postives = 46/74 (62.16%), Query Frame = 0


Query: 95  FSSVGDDVSMEVFEWIDSDSKLLIASEVISRIMNDITSHKFEQERGHVASANECYMTQYG 154
           F S+G   + E FEW+ S  KL+    +  R+MND+  ++ +  RG+V +A  CYM QYG
Sbjct: 468 FMSMGKMATKEAFEWLKSRPKLVQYLSIKGRLMNDLMGYEDDMSRGYVTNAVNCYMKQYG 527

Query: 155 VSEEEAIEELEKQV 169
           V++EEA  EL K V
Sbjct: 528 VTKEEAFRELYKIV 541

[Close]

BLAST of MC10g_new0324 vs. TAIR 10
Match: AT4G20230.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 62.0 bits (149), Expect = 5.5e-10
Identity = 32/72 (44.44%), Postives = 45/72 (62.50%), Query Frame = 0


Query: 97  SVGDDVSMEVFEWIDSDSKLLIASEVISRIMNDITSHKFEQERGHVASANECYMTQYGVS 156
           S+G   + E FEW+ S  KL+ +     R+MNDI   + +  RG+V +A  CYM QYGV+
Sbjct: 472 SMGKTGTKEAFEWLKSRPKLVQSLCTKGRLMNDIAGFEDDMSRGYVVNAVNCYMKQYGVT 531

Query: 157 EEEAIEELEKQV 169
           E+EA +EL K V
Sbjct: 532 EKEAFKELRKMV 543

[Close]

BLAST of MC10g_new0324 vs. TAIR 10
Match: AT3G14540.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 61.2 bits (147), Expect = 9.4e-10
Identity = 32/72 (44.44%), Postives = 45/72 (62.50%), Query Frame = 0


Query: 95  FSSVGDDVSMEVFEWIDSDSKLLIASEVISRIMNDITSHKFEQERGHVASANECYMTQYG 154
           F  + D    E FEW++S+  L+ A  V+ RI ND+ +++ E  RG VA+   CYM QYG
Sbjct: 464 FIGMEDINEKEAFEWLNSNPLLIDALNVLFRIANDVGTYETEINRGEVANGLNCYMKQYG 523

Query: 155 VSEEEAIEELEK 167
           V++EEA  EL K
Sbjct: 524 VTKEEASRELRK 535

The following BLAST results are available for this feature:

BLAST of MC10g_new0324 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6Q3H2	2.0e-20	25.66	Valencene synthase OS=Vitis vinifera OX=29760 GN=ValCS PE=1 SV=1	[more]
Q6Q3H3	4.9e-19	24.34	(-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 S...	[more]
B2KSJ5	2.4e-18	25.66	(+)-gamma-cadinene synthase OS=Cucumis melo OX=3656 PE=1 SV=1	[more]
W0FFD7	7.7e-17	42.86	(-)-drimenol synthase OS=Persicaria hydropiper OX=46901 PE=1 SV=1	[more]
B5A435	3.8e-16	51.81	Sesquiterpene synthase OS=Santalum album OX=35974 PE=1 SV=1	[more]

BLAST of MC10g_new0324 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154020.1	7.93e-66	49.06	(+)-gamma-cadinene synthase-like [Momordica charantia]	[more]
XP_022154019.1	1.85e-61	41.30	(-)-germacrene D synthase-like [Momordica charantia]	[more]
XP_038905736.1	1.26e-51	37.95	(-)-germacrene D synthase-like [Benincasa hispida]	[more]
XP_022154012.1	1.73e-45	80.21	(-)-germacrene D synthase-like [Momordica charantia]	[more]
XP_022144020.1	3.24e-43	34.23	(-)-germacrene D synthase-like [Momordica charantia]	[more]

BLAST of MC10g_new0324 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DIG3	3.84e-66	49.06	(+)-gamma-cadinene synthase-like OS=Momordica charantia OX=3673 GN=LOC111021378 ...	[more]
A0A6J1DKT6	8.95e-62	41.30	(-)-germacrene D synthase-like OS=Momordica charantia OX=3673 GN=LOC111021377 PE...	[more]
A0A6J1DKI4	8.40e-46	80.21	(-)-germacrene D synthase-like OS=Momordica charantia OX=3673 GN=LOC111021373 PE...	[more]
A0A6J1CQG9	1.57e-43	34.23	(-)-germacrene D synthase-like OS=Momordica charantia OX=3673 GN=LOC111013811 PE...	[more]
A0A5D3CJ95	8.06e-43	36.51	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]

BLAST of MC10g_new0324 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G23960.1	4.1e-13	43.37	terpene synthase 21	[more]
AT5G23960.2	4.1e-13	43.37	terpene synthase 21	[more]
AT4G20200.1	2.5e-10	43.24	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]
AT4G20230.1	5.5e-10	44.44	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]
AT3G14540.1	9.4e-10	44.44	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036965	Terpene synthase, N-terminal domain superfamily	GENE3D	1.50.10.130		coord: 2..68 e-value: 4.4E-24 score: 86.7
IPR008949	Isoprenoid synthase domain superfamily	GENE3D	1.10.600.10	Farnesyl Diphosphate Synthase	coord: 69..95 e-value: 4.4E-24 score: 86.7
IPR008949	Isoprenoid synthase domain superfamily	GENE3D	1.10.600.10	Farnesyl Diphosphate Synthase	coord: 96..175 e-value: 3.0E-23 score: 84.1
IPR008949	Isoprenoid synthase domain superfamily	SUPERFAMILY	48576	Terpenoid synthases	coord: 97..171
IPR008949	Isoprenoid synthase domain superfamily	SUPERFAMILY	48576	Terpenoid synthases	coord: 61..97
None	No IPR available	PFAM	PF19086	Terpene_syn_C_2	coord: 97..171 e-value: 4.4E-21 score: 75.6
None	No IPR available	PANTHER	PTHR31225:SF134	(-)-GERMACRENE D SYNTHASE-LIKE	coord: 97..175 coord: 1..96
None	No IPR available	PANTHER	PTHR31225	OS04G0344100 PROTEIN-RELATED	coord: 97..175 coord: 1..96
IPR008930	Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid	SUPERFAMILY	48239	Terpenoid cyclases/Protein prenyltransferases	coord: 2..66

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g_new0324.1	MC10g_new0324.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016102	diterpenoid biosynthetic process
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0010333	terpene synthase activity
molecular_function	GO:0016829	lyase activity