MC10g_new0136 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g_new0136
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	WRKY domain-containing protein
Location	MC10: 5868810 .. 5869830 (+)
RNA-Seq Expression	MC10g_new0136
Synteny	MC10g_new0136

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTAATGGATGGTGACAATATTTCACAGCTGCTGAGTGCACCCACCAATGGTGATATTACACACACACAGCTTCTTCCTCTCAGCCAAAGCTGTGATGAAATTGACTGGGAAGGGCTGCTCTGTGGTTCGTCTTTGAGTTTGGAGATGGAGAAGGCAAGTGGAAGTGAGGGGATAAAAATGGGGGATTTTGATGGTGGAGCTGGAAGAAGAGATGAGGGTAATAATGGAGGGAAAAACAAAGGGAAAATGATGATGGGCAAAAGAAGTAGCAGTGCAGTAGTGCCTAGGATTGCTTTCCAGACAAGGAGTGCTGAGGATGTTCTTGATGATGGCTACAGATGGAGGAAATATGGTCAGAAGGCTGTCAAACATAGCACACATCCCAGGTACATGTATATGTTTTTGTTTTGTTTGTTGTCATTTTGTCTCAGTCTTCAGATTTGTGAAGAGAAAATGGAGTGCCCAAACACTAATATAAGTTGACGAAAGGGGGAAAAAAGAAATGAAAGTGGTTCAAAGATGCATGTTATTTGAGAATGTGGGCATCAATCCGCGAGCTTTTTTTTTTAATGTTGTTGAGTTGAACGTGAGAGTTGAACTACACTTAAATTGACAAGTAATAGATAATTATGTTGATTACTCAAATCATGCGTGTGTGTATAATTTCTTTCCTTATAAGTTGATGGAAGAAAAACACGAAAAGAAAGGTAAAATTTGTTATTGTGTCTACTAAATTGATGTATACTAATAACTTAATATGAGTTAACATGTTAAAAAAAACTTGTTTAAATTCTCACTTTGGAAAGTGGGTTTCTTTTTCTTTTGTTTGATTTGTTTTAGGAGCTATTACCGGTGTACACATCATACATGCAACGTTAAGAAACAAATTCAAAGGCACTCAAAGGATCCAACCATAGTGGTGACAACATATGAAGGGATTCACAACCATCCATCTGAAAAATTAATGGAGACTCTAAGCCCTCTCCTCAAGCAATTGCAGTTCCTTTCTGGCATTTAG

mRNA sequence

ATGGTAATGGATGGTGACAATATTTCACAGCTGCTGAGTGCACCCACCAATGGTGATATTACACACACACAGCTTCTTCCTCTCAGCCAAAGCTGTGATGAAATTGACTGGGAAGGGCTGCTCTGTGGTTCGTCTTTGAGTTTGGAGATGGAGAAGGCAAGTGGAAGTGAGGGGATAAAAATGGGGGATTTTGATGGTGGAGCTGGAAGAAGAGATGAGGGTAATAATGGAGGGAAAAACAAAGGGAAAATGATGATGGGCAAAAGAAGTAGCAGTGCAGTAGTGCCTAGGATTGCTTTCCAGACAAGGAGTGCTGAGGATGTTCTTGATGATGGCTACAGATGGAGGAAATATGGTCAGAAGGCTGTCAAACATAGCACACATCCCAGGAGCTATTACCGGTGTACACATCATACATGCAACGTTAAGAAACAAATTCAAAGGCACTCAAAGGATCCAACCATAGTGGTGACAACATATGAAGGGATTCACAACCATCCATCTGAAAAATTAATGGAGACTCTAAGCCCTCTCCTCAAGCAATTGCAGTTCCTTTCTGGCATTTAG

Coding sequence (CDS)

ATGGTAATGGATGGTGACAATATTTCACAGCTGCTGAGTGCACCCACCAATGGTGATATTACACACACACAGCTTCTTCCTCTCAGCCAAAGCTGTGATGAAATTGACTGGGAAGGGCTGCTCTGTGGTTCGTCTTTGAGTTTGGAGATGGAGAAGGCAAGTGGAAGTGAGGGGATAAAAATGGGGGATTTTGATGGTGGAGCTGGAAGAAGAGATGAGGGTAATAATGGAGGGAAAAACAAAGGGAAAATGATGATGGGCAAAAGAAGTAGCAGTGCAGTAGTGCCTAGGATTGCTTTCCAGACAAGGAGTGCTGAGGATGTTCTTGATGATGGCTACAGATGGAGGAAATATGGTCAGAAGGCTGTCAAACATAGCACACATCCCAGGAGCTATTACCGGTGTACACATCATACATGCAACGTTAAGAAACAAATTCAAAGGCACTCAAAGGATCCAACCATAGTGGTGACAACATATGAAGGGATTCACAACCATCCATCTGAAAAATTAATGGAGACTCTAAGCCCTCTCCTCAAGCAATTGCAGTTCCTTTCTGGCATTTAG

Protein sequence

MVMDGDNISQLLSAPTNGDITHTQLLPLSQSCDEIDWEGLLCGSSLSLEMEKASGSEGIKMGDFDGGAGRRDEGNNGGKNKGKMMMGKRSSSAVVPRIAFQTRSAEDVLDDGYRWRKYGQKAVKHSTHPRSYYRCTHHTCNVKKQIQRHSKDPTIVVTTYEGIHNHPSEKLMETLSPLLKQLQFLSGI

Homology

[Close]

BLAST of MC10g_new0136 vs. ExPASy Swiss-Prot
Match: Q9FFS3 (Probable WRKY transcription factor 24 OS=Arabidopsis thaliana OX=3702 GN=WRKY24 PE=1 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 2.0e-42
Identity = 82/106 (77.36%), Postives = 93/106 (87.74%), Query Frame = 0


Query: 81  KGKMMMGKRSSSAVVPRIAFQTRSAEDVLDDGYRWRKYGQKAVKHSTHPRSYYRCTHHTC 140
           KGK +  KRS    VPRIAF TRS +DVLDDGYRWRKYGQK+VKH+ HPRSYYRCT+HTC
Sbjct: 71  KGKELKEKRSRK--VPRIAFHTRSDDDVLDDGYRWRKYGQKSVKHNAHPRSYYRCTYHTC 130

Query: 141 NVKKQIQRHSKDPTIVVTTYEGIHNHPSEKLMETLSPLLKQLQFLS 187
           NVKKQ+QR +KDP +VVTTYEG+HNHP EKLMETL+PLL+QLQFLS
Sbjct: 131 NVKKQVQRLAKDPNVVVTTYEGVHNHPCEKLMETLNPLLRQLQFLS 174

[Close]

BLAST of MC10g_new0136 vs. ExPASy Swiss-Prot
Match: Q8VWQ4 (Probable WRKY transcription factor 56 OS=Arabidopsis thaliana OX=3702 GN=WRKY56 PE=1 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 5.7e-42
Identity = 96/186 (51.61%), Postives = 119/186 (63.98%), Query Frame = 0


Query: 6   DNISQLLSAPT---NGDITHTQLLPLSQSCDEIDWEGLLCGSSLSLEMEKASGSEGIKMG 65
           D+ + ++ AP+   +GD+      P S SC          G  LS ++E   G  G   G
Sbjct: 26  DDKTLMMMAPSLIFSGDVG-----PSSSSCTP-------AGYHLSAQLENFRGGGGEMGG 85

Query: 66  DFDGGAGRRDEGNNGGKNKGKMMMGKRSSSAVVPRIAFQTRSAEDVLDDGYRWRKYGQKA 125
                +   D   N  K KGK  +        + RIAF TRS +DVLDDGYRWRKYGQK+
Sbjct: 86  LVSNNSNNSDHNKNCNKGKGKRTL-------AMQRIAFHTRSDDDVLDDGYRWRKYGQKS 145

Query: 126 VKHSTHPRSYYRCTHHTCNVKKQIQRHSKDPTIVVTTYEGIHNHPSEKLMETLSPLLKQL 185
           VK++ HPRSYYRCT+HTCNVKKQ+QR +KDP +VVTTYEG+HNHP EKLMETLSPLL+QL
Sbjct: 146 VKNNAHPRSYYRCTYHTCNVKKQVQRLAKDPNVVVTTYEGVHNHPCEKLMETLSPLLRQL 192

Query: 186 QFLSGI 189
           QFLS +
Sbjct: 206 QFLSRV 192

[Close]

BLAST of MC10g_new0136 vs. ExPASy Swiss-Prot
Match: Q8GY11 (Probable WRKY transcription factor 43 OS=Arabidopsis thaliana OX=3702 GN=WRKY43 PE=1 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 1.5e-34
Identity = 67/91 (73.63%), Postives = 80/91 (87.91%), Query Frame = 0


Query: 96  PRIAFQTRSAEDVLDDGYRWRKYGQKAVKHSTHPRSYYRCTHHTCNVKKQIQRHSKDPTI 155
           PR +F+T+S  D+LDDGYRWRKYGQK+VK+S +PRSYYRCT H CNVKKQ+QR SK+ +I
Sbjct: 16  PRFSFRTKSDADILDDGYRWRKYGQKSVKNSLYPRSYYRCTQHMCNVKKQVQRLSKETSI 75

Query: 156 VVTTYEGIHNHPSEKLMETLSPLLKQLQFLS 187
           V TTYEGIHNHP E+LM+TL+PLL QLQFLS
Sbjct: 76  VETTYEGIHNHPCEELMQTLTPLLHQLQFLS 106

[Close]

BLAST of MC10g_new0136 vs. ExPASy Swiss-Prot
Match: Q9FYA2 (Probable WRKY transcription factor 75 OS=Arabidopsis thaliana OX=3702 GN=WRKY75 PE=1 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.0e-27
Identity = 58/90 (64.44%), Postives = 69/90 (76.67%), Query Frame = 0


Query: 97  RIAFQTRSAEDVLDDGYRWRKYGQKAVKHSTHPRSYYRCTHHTCNVKKQIQRHSKDPTIV 156
           R AFQTRS  D+LDDGYRWRKYGQKAVK++  PRSYYRCT+  CNVKKQ+QR + D  +V
Sbjct: 54  RYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTYGGCNVKKQVQRLTVDQEVV 113

Query: 157 VTTYEGIHNHPSEKLMETLSPLLKQLQFLS 187
           VTTYEG+H+HP EK  E    +L Q+Q  S
Sbjct: 114 VTTYEGVHSHPIEKSTENFEHILTQMQIYS 143

[Close]

BLAST of MC10g_new0136 vs. ExPASy Swiss-Prot
Match: Q8VWJ2 (WRKY transcription factor 28 OS=Arabidopsis thaliana OX=3702 GN=WRKY28 PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.1e-24
Identity = 61/119 (51.26%), Postives = 80/119 (67.23%), Query Frame = 0


Query: 53  ASGSEGIKMGDFDGGAGRRDEGNNGGKNKGKMMMGKRSSSAV----VPRIAFQTRSAEDV 112
           +S SE    G+ D G  RR     G +++    +GK   + V     PR++F T+S  D 
Sbjct: 112 SSSSEADHPGE-DSGKSRRKRELVGEEDQISKKVGKTKKTEVKKQREPRVSFMTKSEVDH 171

Query: 113 LDDGYRWRKYGQKAVKHSTHPRSYYRCTHHTCNVKKQIQRHSKDPTIVVTTYEGIHNHP 168
           L+DGYRWRKYGQKAVK+S +PRSYYRCT   CNVKK+++R  +DPT+V+TTYEG HNHP
Sbjct: 172 LEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVKKRVERSFQDPTVVITTYEGQHNHP 229

[Close]

BLAST of MC10g_new0136 vs. NCBI nr
Match: XP_022136661.1 (probable WRKY transcription factor 24 [Momordica charantia])

HSP 1 Score: 379 bits (974), Expect = 8.86e-133
Identity = 188/188 (100.00%), Postives = 188/188 (100.00%), Query Frame = 0


Query: 1   MVMDGDNISQLLSAPTNGDITHTQLLPLSQSCDEIDWEGLLCGSSLSLEMEKASGSEGIK 60
           MVMDGDNISQLLSAPTNGDITHTQLLPLSQSCDEIDWEGLLCGSSLSLEMEKASGSEGIK
Sbjct: 1   MVMDGDNISQLLSAPTNGDITHTQLLPLSQSCDEIDWEGLLCGSSLSLEMEKASGSEGIK 60

Query: 61  MGDFDGGAGRRDEGNNGGKNKGKMMMGKRSSSAVVPRIAFQTRSAEDVLDDGYRWRKYGQ 120
           MGDFDGGAGRRDEGNNGGKNKGKMMMGKRSSSAVVPRIAFQTRSAEDVLDDGYRWRKYGQ
Sbjct: 61  MGDFDGGAGRRDEGNNGGKNKGKMMMGKRSSSAVVPRIAFQTRSAEDVLDDGYRWRKYGQ 120

Query: 121 KAVKHSTHPRSYYRCTHHTCNVKKQIQRHSKDPTIVVTTYEGIHNHPSEKLMETLSPLLK 180
           KAVKHSTHPRSYYRCTHHTCNVKKQIQRHSKDPTIVVTTYEGIHNHPSEKLMETLSPLLK
Sbjct: 121 KAVKHSTHPRSYYRCTHHTCNVKKQIQRHSKDPTIVVTTYEGIHNHPSEKLMETLSPLLK 180

Query: 181 QLQFLSGI 188
           QLQFLSGI
Sbjct: 181 QLQFLSGI 188

[Close]

BLAST of MC10g_new0136 vs. NCBI nr
Match: AZU90778.1 (WRKY transcription factor 3 [Siraitia grosvenorii] >QEE82237.1 WRKY transcription factor 3 [Siraitia grosvenorii])

HSP 1 Score: 240 bits (613), Expect = 1.12e-77
Identity = 144/207 (69.57%), Postives = 156/207 (75.36%), Query Frame = 0


Query: 1   MVMDGDNISQLLSAPTNGD---------------ITHTQLLPLSQS----CDEIDWEGLL 60
           M MDGD I QLL+APT  D               I  TQL PL+++     D+IDW GLL
Sbjct: 1   MDMDGD-IPQLLTAPTIADMATSSSSSYPSSSSLINCTQL-PLNENSDAAADDIDWVGLL 60

Query: 61  CGSSLSLEMEKASGSEGIKMGDFDGGAGRRDEGNNGGKNKGKMMMGKRSSSAVVPRIAFQ 120
              SLSLEMEK  GSE + M D  GG     EG  GGKNKGKM+ GKRS+   +PRIAFQ
Sbjct: 61  SAGSLSLEMEKPCGSE-VAMADDVGGR----EGY-GGKNKGKMV-GKRSA---LPRIAFQ 120

Query: 121 TRSAEDVLDDGYRWRKYGQKAVKHSTHPRSYYRCTHHTCNVKKQIQRHSKDPTIVVTTYE 180
           TRSAEDVLDDGYRWRKYGQKAVKHS++PRSYYRCTHHTCNVKKQIQRHSKDP IVVTTYE
Sbjct: 121 TRSAEDVLDDGYRWRKYGQKAVKHSSYPRSYYRCTHHTCNVKKQIQRHSKDPNIVVTTYE 180

Query: 181 GIHNHPSEKLMETLSPLLKQLQFLSGI 188
           G+HNHPSEKLMETLSPLLKQLQFLSGI
Sbjct: 181 GVHNHPSEKLMETLSPLLKQLQFLSGI 195

[Close]

BLAST of MC10g_new0136 vs. NCBI nr
Match: KAG7031188.1 (putative WRKY transcription factor 24, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 235 bits (599), Expect = 9.77e-76
Identity = 137/197 (69.54%), Postives = 153/197 (77.66%), Query Frame = 0


Query: 1   MVMDGD--NISQLLSAPTNGDITHTQLLPLSQSCDEIDWEGLLCGSS-LSLEMEKASG-S 60
           MVMDGD  N  ++ S+ T     + Q    S    EIDW G+  GSS +SLEMEK SG S
Sbjct: 1   MVMDGDIHNSHEMASSST----VNYQ----SSDAAEIDWVGIFGGSSDVSLEMEKGSGVS 60

Query: 61  EG--IKMGDFDGGAGRRDEGN---NGGKNKGKMMMGKRSSSAVVPRIAFQTRSAEDVLDD 120
           EG  + MGD +     ++E +   +GGKNKGKM MGKR +   +PR+AFQTRSAEDVLDD
Sbjct: 61  EGAIMAMGDCN----YQNESHVHVHGGKNKGKMAMGKRGA---IPRVAFQTRSAEDVLDD 120

Query: 121 GYRWRKYGQKAVKHSTHPRSYYRCTHHTCNVKKQIQRHSKDPTIVVTTYEGIHNHPSEKL 180
           GYRWRKYGQKAVKHSTHPRSYYRCTHHTCNVKKQIQRHSKDPTIVVTTYEGIHNHPSEKL
Sbjct: 121 GYRWRKYGQKAVKHSTHPRSYYRCTHHTCNVKKQIQRHSKDPTIVVTTYEGIHNHPSEKL 180

Query: 181 METLSPLLKQLQFLSGI 188
           METL+PLLKQLQFLSGI
Sbjct: 181 METLTPLLKQLQFLSGI 182

[Close]

BLAST of MC10g_new0136 vs. NCBI nr
Match: XP_023544319.1 (probable WRKY transcription factor 24 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 235 bits (599), Expect = 1.01e-75
Identity = 136/197 (69.04%), Postives = 153/197 (77.66%), Query Frame = 0


Query: 1   MVMDGD--NISQLLSAPTNGDITHTQLLPLSQSCDEIDWEGLLCGSS-LSLEMEKASG-S 60
           MVMDGD  N   + S+ T   + +      S +  EIDW G+  GSS +SLEMEK SG S
Sbjct: 1   MVMDGDIHNSHDMASSST---VNYQS----SDAAAEIDWVGIFGGSSDVSLEMEKGSGVS 60

Query: 61  EG--IKMGDFDGGAGRRDEGN---NGGKNKGKMMMGKRSSSAVVPRIAFQTRSAEDVLDD 120
           EG  + MGD +     ++E +   +GGKNKGKM MGKR +   +PR+AFQTRSAEDVLDD
Sbjct: 61  EGAIMAMGDCN----YQNESHVHVHGGKNKGKMAMGKRGA---IPRVAFQTRSAEDVLDD 120

Query: 121 GYRWRKYGQKAVKHSTHPRSYYRCTHHTCNVKKQIQRHSKDPTIVVTTYEGIHNHPSEKL 180
           GYRWRKYGQKAVKHSTHPRSYYRCTHHTCNVKKQIQRHSKDPTIVVTTYEGIHNHPSEKL
Sbjct: 121 GYRWRKYGQKAVKHSTHPRSYYRCTHHTCNVKKQIQRHSKDPTIVVTTYEGIHNHPSEKL 180

Query: 181 METLSPLLKQLQFLSGI 188
           METL+PLLKQLQFLSGI
Sbjct: 181 METLTPLLKQLQFLSGI 183

[Close]

BLAST of MC10g_new0136 vs. NCBI nr
Match: XP_022942320.1 (probable WRKY transcription factor 43 [Cucurbita moschata])

HSP 1 Score: 229 bits (585), Expect = 8.83e-74
Identity = 126/167 (75.45%), Postives = 139/167 (83.23%), Query Frame = 0


Query: 29  SQSCDEIDWEGLLCGSS-LSLEMEKASG-SEG--IKMGDFDGGAGRRDEGN---NGGKNK 88
           S    EIDW G+  GSS +SLEMEK SG SEG  + MGD +     ++E +   +GGKNK
Sbjct: 11  SSDAAEIDWVGIFGGSSDVSLEMEKGSGVSEGAIMAMGDCN----YQNESHVHVHGGKNK 70

Query: 89  GKMMMGKRSSSAVVPRIAFQTRSAEDVLDDGYRWRKYGQKAVKHSTHPRSYYRCTHHTCN 148
           GKM MGKR +   +PR+AFQTRSAEDVLDDGYRWRKYGQKAVKHST+PRSYYRCTHHTCN
Sbjct: 71  GKMAMGKRGA---IPRVAFQTRSAEDVLDDGYRWRKYGQKAVKHSTYPRSYYRCTHHTCN 130

Query: 149 VKKQIQRHSKDPTIVVTTYEGIHNHPSEKLMETLSPLLKQLQFLSGI 188
           VKKQIQRHSKDPTIVVTTYEGIHNHPSEKLMETL+PLLKQLQFLSGI
Sbjct: 131 VKKQIQRHSKDPTIVVTTYEGIHNHPSEKLMETLTPLLKQLQFLSGI 170

[Close]

BLAST of MC10g_new0136 vs. ExPASy TrEMBL
Match: A0A6J1C449 (probable WRKY transcription factor 24 OS=Momordica charantia OX=3673 GN=LOC111008314 PE=4 SV=1)

HSP 1 Score: 379 bits (974), Expect = 4.29e-133
Identity = 188/188 (100.00%), Postives = 188/188 (100.00%), Query Frame = 0


Query: 1   MVMDGDNISQLLSAPTNGDITHTQLLPLSQSCDEIDWEGLLCGSSLSLEMEKASGSEGIK 60
           MVMDGDNISQLLSAPTNGDITHTQLLPLSQSCDEIDWEGLLCGSSLSLEMEKASGSEGIK
Sbjct: 1   MVMDGDNISQLLSAPTNGDITHTQLLPLSQSCDEIDWEGLLCGSSLSLEMEKASGSEGIK 60

Query: 61  MGDFDGGAGRRDEGNNGGKNKGKMMMGKRSSSAVVPRIAFQTRSAEDVLDDGYRWRKYGQ 120
           MGDFDGGAGRRDEGNNGGKNKGKMMMGKRSSSAVVPRIAFQTRSAEDVLDDGYRWRKYGQ
Sbjct: 61  MGDFDGGAGRRDEGNNGGKNKGKMMMGKRSSSAVVPRIAFQTRSAEDVLDDGYRWRKYGQ 120

Query: 121 KAVKHSTHPRSYYRCTHHTCNVKKQIQRHSKDPTIVVTTYEGIHNHPSEKLMETLSPLLK 180
           KAVKHSTHPRSYYRCTHHTCNVKKQIQRHSKDPTIVVTTYEGIHNHPSEKLMETLSPLLK
Sbjct: 121 KAVKHSTHPRSYYRCTHHTCNVKKQIQRHSKDPTIVVTTYEGIHNHPSEKLMETLSPLLK 180

Query: 181 QLQFLSGI 188
           QLQFLSGI
Sbjct: 181 QLQFLSGI 188

[Close]

BLAST of MC10g_new0136 vs. ExPASy TrEMBL
Match: A0A3Q9QZY9 (WRKY transcription factor 3 OS=Siraitia grosvenorii OX=190515 GN=WRKY3 PE=2 SV=1)

HSP 1 Score: 240 bits (613), Expect = 5.42e-78
Identity = 144/207 (69.57%), Postives = 156/207 (75.36%), Query Frame = 0


Query: 1   MVMDGDNISQLLSAPTNGD---------------ITHTQLLPLSQS----CDEIDWEGLL 60
           M MDGD I QLL+APT  D               I  TQL PL+++     D+IDW GLL
Sbjct: 1   MDMDGD-IPQLLTAPTIADMATSSSSSYPSSSSLINCTQL-PLNENSDAAADDIDWVGLL 60

Query: 61  CGSSLSLEMEKASGSEGIKMGDFDGGAGRRDEGNNGGKNKGKMMMGKRSSSAVVPRIAFQ 120
              SLSLEMEK  GSE + M D  GG     EG  GGKNKGKM+ GKRS+   +PRIAFQ
Sbjct: 61  SAGSLSLEMEKPCGSE-VAMADDVGGR----EGY-GGKNKGKMV-GKRSA---LPRIAFQ 120

Query: 121 TRSAEDVLDDGYRWRKYGQKAVKHSTHPRSYYRCTHHTCNVKKQIQRHSKDPTIVVTTYE 180
           TRSAEDVLDDGYRWRKYGQKAVKHS++PRSYYRCTHHTCNVKKQIQRHSKDP IVVTTYE
Sbjct: 121 TRSAEDVLDDGYRWRKYGQKAVKHSSYPRSYYRCTHHTCNVKKQIQRHSKDPNIVVTTYE 180

Query: 181 GIHNHPSEKLMETLSPLLKQLQFLSGI 188
           G+HNHPSEKLMETLSPLLKQLQFLSGI
Sbjct: 181 GVHNHPSEKLMETLSPLLKQLQFLSGI 195

[Close]

BLAST of MC10g_new0136 vs. ExPASy TrEMBL
Match: A0A6J1FW26 (probable WRKY transcription factor 43 OS=Cucurbita moschata OX=3662 GN=LOC111447402 PE=4 SV=1)

HSP 1 Score: 229 bits (585), Expect = 4.28e-74
Identity = 126/167 (75.45%), Postives = 139/167 (83.23%), Query Frame = 0


Query: 29  SQSCDEIDWEGLLCGSS-LSLEMEKASG-SEG--IKMGDFDGGAGRRDEGN---NGGKNK 88
           S    EIDW G+  GSS +SLEMEK SG SEG  + MGD +     ++E +   +GGKNK
Sbjct: 11  SSDAAEIDWVGIFGGSSDVSLEMEKGSGVSEGAIMAMGDCN----YQNESHVHVHGGKNK 70

Query: 89  GKMMMGKRSSSAVVPRIAFQTRSAEDVLDDGYRWRKYGQKAVKHSTHPRSYYRCTHHTCN 148
           GKM MGKR +   +PR+AFQTRSAEDVLDDGYRWRKYGQKAVKHST+PRSYYRCTHHTCN
Sbjct: 71  GKMAMGKRGA---IPRVAFQTRSAEDVLDDGYRWRKYGQKAVKHSTYPRSYYRCTHHTCN 130

Query: 149 VKKQIQRHSKDPTIVVTTYEGIHNHPSEKLMETLSPLLKQLQFLSGI 188
           VKKQIQRHSKDPTIVVTTYEGIHNHPSEKLMETL+PLLKQLQFLSGI
Sbjct: 131 VKKQIQRHSKDPTIVVTTYEGIHNHPSEKLMETLTPLLKQLQFLSGI 170

[Close]

BLAST of MC10g_new0136 vs. ExPASy TrEMBL
Match: A0A6J1J4I8 (probable WRKY transcription factor 43 OS=Cucurbita maxima OX=3661 GN=LOC111481692 PE=4 SV=1)

HSP 1 Score: 227 bits (579), Expect = 3.86e-73
Identity = 123/162 (75.93%), Postives = 137/162 (84.57%), Query Frame = 0


Query: 34  EIDWEGLLCG-SSLSLEMEKASG-SEG--IKMGDFDGGAGRRDEGN---NGGKNKGKMMM 93
           EIDW G+  G S +SLEMEK SG SEG  + MGD++     +++ +   +GGKNKGKM M
Sbjct: 19  EIDWVGIFGGYSDVSLEMEKGSGVSEGATMAMGDYN----YQNDTHVHVHGGKNKGKMAM 78

Query: 94  GKRSSSAVVPRIAFQTRSAEDVLDDGYRWRKYGQKAVKHSTHPRSYYRCTHHTCNVKKQI 153
            KR +   +PR+AFQTRSAEDVLDDGYRWRKYGQKAVKHSTHPRSYYRCTHHTCNVKKQI
Sbjct: 79  AKRGA---IPRVAFQTRSAEDVLDDGYRWRKYGQKAVKHSTHPRSYYRCTHHTCNVKKQI 138

Query: 154 QRHSKDPTIVVTTYEGIHNHPSEKLMETLSPLLKQLQFLSGI 188
           QRHSKDPTIVVTTYEGIHNHPSEKLMETL+PLLKQLQFLSGI
Sbjct: 139 QRHSKDPTIVVTTYEGIHNHPSEKLMETLTPLLKQLQFLSGI 173

[Close]

BLAST of MC10g_new0136 vs. ExPASy TrEMBL
Match: A0A1S3BSW1 (probable WRKY transcription factor 43 OS=Cucumis melo OX=3656 GN=LOC103493140 PE=4 SV=1)

HSP 1 Score: 223 bits (568), Expect = 5.05e-71
Identity = 126/213 (59.15%), Postives = 141/213 (66.20%), Query Frame = 0


Query: 3   MDGDNISQLLSAPTNGDITHTQLLPLSQSCDEIDWEGLLCGSSLS---LEMEKAS--GSE 62
           M  DNI+Q+ +  ++          +  +C+ IDW+GL   S LS   LEM +    GSE
Sbjct: 1   MGDDNINQMGTTSSSS---------MMNNCEIIDWDGLFSSSCLSNNNLEMVEKGICGSE 60

Query: 63  ----------------------GIKMGDFDGGAGRRDEGNNGGKNKGKMMMGKRSSSAVV 122
                                 G+K GD     G     NN  K KG+M+MGKR S+   
Sbjct: 61  DDNNYYPMGDSNYDNNNNVVIGGVKEGDIVISGGNNHNNNNNIKYKGRMVMGKRRSAMAS 120

Query: 123 PRIAFQTRSAEDVLDDGYRWRKYGQKAVKHSTHPRSYYRCTHHTCNVKKQIQRHSKDPTI 182
           PRIAFQTRS EDVLDDGYRWRKYGQKAVKHS HPRSYYRCTHHTCNVKKQIQRHSKDPTI
Sbjct: 121 PRIAFQTRSVEDVLDDGYRWRKYGQKAVKHSNHPRSYYRCTHHTCNVKKQIQRHSKDPTI 180

Query: 183 VVTTYEGIHNHPSEKLMETLSPLLKQLQFLSGI 188
           VVTTYEGIHNHPSEKLMETL+PLLKQLQFLSGI
Sbjct: 181 VVTTYEGIHNHPSEKLMETLTPLLKQLQFLSGI 204

[Close]

BLAST of MC10g_new0136 vs. TAIR 10
Match: AT5G41570.1 (WRKY DNA-binding protein 24 )

HSP 1 Score: 173.7 bits (439), Expect = 1.4e-43
Identity = 82/106 (77.36%), Postives = 93/106 (87.74%), Query Frame = 0


Query: 81  KGKMMMGKRSSSAVVPRIAFQTRSAEDVLDDGYRWRKYGQKAVKHSTHPRSYYRCTHHTC 140
           KGK +  KRS    VPRIAF TRS +DVLDDGYRWRKYGQK+VKH+ HPRSYYRCT+HTC
Sbjct: 71  KGKELKEKRSRK--VPRIAFHTRSDDDVLDDGYRWRKYGQKSVKHNAHPRSYYRCTYHTC 130

Query: 141 NVKKQIQRHSKDPTIVVTTYEGIHNHPSEKLMETLSPLLKQLQFLS 187
           NVKKQ+QR +KDP +VVTTYEG+HNHP EKLMETL+PLL+QLQFLS
Sbjct: 131 NVKKQVQRLAKDPNVVVTTYEGVHNHPCEKLMETLNPLLRQLQFLS 174

[Close]

BLAST of MC10g_new0136 vs. TAIR 10
Match: AT1G64000.1 (WRKY DNA-binding protein 56 )

HSP 1 Score: 172.2 bits (435), Expect = 4.0e-43
Identity = 96/186 (51.61%), Postives = 119/186 (63.98%), Query Frame = 0


Query: 6   DNISQLLSAPT---NGDITHTQLLPLSQSCDEIDWEGLLCGSSLSLEMEKASGSEGIKMG 65
           D+ + ++ AP+   +GD+      P S SC          G  LS ++E   G  G   G
Sbjct: 26  DDKTLMMMAPSLIFSGDVG-----PSSSSCTP-------AGYHLSAQLENFRGGGGEMGG 85

Query: 66  DFDGGAGRRDEGNNGGKNKGKMMMGKRSSSAVVPRIAFQTRSAEDVLDDGYRWRKYGQKA 125
                +   D   N  K KGK  +        + RIAF TRS +DVLDDGYRWRKYGQK+
Sbjct: 86  LVSNNSNNSDHNKNCNKGKGKRTL-------AMQRIAFHTRSDDDVLDDGYRWRKYGQKS 145

Query: 126 VKHSTHPRSYYRCTHHTCNVKKQIQRHSKDPTIVVTTYEGIHNHPSEKLMETLSPLLKQL 185
           VK++ HPRSYYRCT+HTCNVKKQ+QR +KDP +VVTTYEG+HNHP EKLMETLSPLL+QL
Sbjct: 146 VKNNAHPRSYYRCTYHTCNVKKQVQRLAKDPNVVVTTYEGVHNHPCEKLMETLSPLLRQL 192

Query: 186 QFLSGI 189
           QFLS +
Sbjct: 206 QFLSRV 192

[Close]

BLAST of MC10g_new0136 vs. TAIR 10
Match: AT2G46130.1 (WRKY DNA-binding protein 43 )

HSP 1 Score: 147.5 bits (371), Expect = 1.1e-35
Identity = 67/91 (73.63%), Postives = 80/91 (87.91%), Query Frame = 0


Query: 96  PRIAFQTRSAEDVLDDGYRWRKYGQKAVKHSTHPRSYYRCTHHTCNVKKQIQRHSKDPTI 155
           PR +F+T+S  D+LDDGYRWRKYGQK+VK+S +PRSYYRCT H CNVKKQ+QR SK+ +I
Sbjct: 16  PRFSFRTKSDADILDDGYRWRKYGQKSVKNSLYPRSYYRCTQHMCNVKKQVQRLSKETSI 75

Query: 156 VVTTYEGIHNHPSEKLMETLSPLLKQLQFLS 187
           V TTYEGIHNHP E+LM+TL+PLL QLQFLS
Sbjct: 76  VETTYEGIHNHPCEELMQTLTPLLHQLQFLS 106

[Close]

BLAST of MC10g_new0136 vs. TAIR 10
Match: AT2G46130.2 (WRKY DNA-binding protein 43 )

HSP 1 Score: 133.7 bits (335), Expect = 1.6e-31
Identity = 63/91 (69.23%), Postives = 76/91 (83.52%), Query Frame = 0


Query: 96  PRIAFQTRSAEDVLDDGYRWRKYGQKAVKHSTHPRSYYRCTHHTCNVKKQIQRHSKDPTI 155
           PR +F+T+S  D+LDDGYRWRKYGQK+VK+S +P    RCT H CNVKKQ+QR SK+ +I
Sbjct: 4   PRFSFRTKSDADILDDGYRWRKYGQKSVKNSLYP----RCTQHMCNVKKQVQRLSKETSI 63

Query: 156 VVTTYEGIHNHPSEKLMETLSPLLKQLQFLS 187
           V TTYEGIHNHP E+LM+TL+PLL QLQFLS
Sbjct: 64  VETTYEGIHNHPCEELMQTLTPLLHQLQFLS 90

[Close]

BLAST of MC10g_new0136 vs. TAIR 10
Match: AT5G13080.1 (WRKY DNA-binding protein 75 )

HSP 1 Score: 124.8 bits (312), Expect = 7.4e-29
Identity = 58/90 (64.44%), Postives = 69/90 (76.67%), Query Frame = 0


Query: 97  RIAFQTRSAEDVLDDGYRWRKYGQKAVKHSTHPRSYYRCTHHTCNVKKQIQRHSKDPTIV 156
           R AFQTRS  D+LDDGYRWRKYGQKAVK++  PRSYYRCT+  CNVKKQ+QR + D  +V
Sbjct: 54  RYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTYGGCNVKKQVQRLTVDQEVV 113

Query: 157 VTTYEGIHNHPSEKLMETLSPLLKQLQFLS 187
           VTTYEG+H+HP EK  E    +L Q+Q  S
Sbjct: 114 VTTYEGVHSHPIEKSTENFEHILTQMQIYS 143

The following BLAST results are available for this feature:

BLAST of MC10g_new0136 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FFS3	2.0e-42	77.36	Probable WRKY transcription factor 24 OS=Arabidopsis thaliana OX=3702 GN=WRKY24 ...	[more]
Q8VWQ4	5.7e-42	51.61	Probable WRKY transcription factor 56 OS=Arabidopsis thaliana OX=3702 GN=WRKY56 ...	[more]
Q8GY11	1.5e-34	73.63	Probable WRKY transcription factor 43 OS=Arabidopsis thaliana OX=3702 GN=WRKY43 ...	[more]
Q9FYA2	1.0e-27	64.44	Probable WRKY transcription factor 75 OS=Arabidopsis thaliana OX=3702 GN=WRKY75 ...	[more]
Q8VWJ2	3.1e-24	51.26	WRKY transcription factor 28 OS=Arabidopsis thaliana OX=3702 GN=WRKY28 PE=2 SV=1	[more]

BLAST of MC10g_new0136 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022136661.1	8.86e-133	100.00	probable WRKY transcription factor 24 [Momordica charantia]	[more]
AZU90778.1	1.12e-77	69.57	WRKY transcription factor 3 [Siraitia grosvenorii] >QEE82237.1 WRKY transcriptio...	[more]
KAG7031188.1	9.77e-76	69.54	putative WRKY transcription factor 24, partial [Cucurbita argyrosperma subsp. ar...	[more]
XP_023544319.1	1.01e-75	69.04	probable WRKY transcription factor 24 [Cucurbita pepo subsp. pepo]	[more]
XP_022942320.1	8.83e-74	75.45	probable WRKY transcription factor 43 [Cucurbita moschata]	[more]

BLAST of MC10g_new0136 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C449	4.29e-133	100.00	probable WRKY transcription factor 24 OS=Momordica charantia OX=3673 GN=LOC11100...	[more]
A0A3Q9QZY9	5.42e-78	69.57	WRKY transcription factor 3 OS=Siraitia grosvenorii OX=190515 GN=WRKY3 PE=2 SV=1	[more]
A0A6J1FW26	4.28e-74	75.45	probable WRKY transcription factor 43 OS=Cucurbita moschata OX=3662 GN=LOC111447...	[more]
A0A6J1J4I8	3.86e-73	75.93	probable WRKY transcription factor 43 OS=Cucurbita maxima OX=3661 GN=LOC11148169...	[more]
A0A1S3BSW1	5.05e-71	59.15	probable WRKY transcription factor 43 OS=Cucumis melo OX=3656 GN=LOC103493140 PE...	[more]

BLAST of MC10g_new0136 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G41570.1	1.4e-43	77.36	WRKY DNA-binding protein 24	[more]
AT1G64000.1	4.0e-43	51.61	WRKY DNA-binding protein 56	[more]
AT2G46130.1	1.1e-35	73.63	WRKY DNA-binding protein 43	[more]
AT2G46130.2	1.6e-31	69.23	WRKY DNA-binding protein 43	[more]
AT5G13080.1	7.4e-29	64.44	WRKY DNA-binding protein 75	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003657	WRKY domain	SMART	SM00774	WRKY_cls	coord: 109..168 e-value: 2.6E-36 score: 136.6
IPR003657	WRKY domain	PFAM	PF03106	WRKY	coord: 110..167 e-value: 1.0E-24 score: 86.6
IPR003657	WRKY domain	PROSITE	PS50811	WRKY	coord: 104..169 score: 29.229555
IPR036576	WRKY domain superfamily	GENE3D	2.20.25.80	WRKY domain	coord: 93..168 e-value: 2.2E-33 score: 115.8
IPR036576	WRKY domain superfamily	SUPERFAMILY	118290	WRKY DNA-binding domain	coord: 102..168
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 57..89
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 64..78
None	No IPR available	PANTHER	PTHR31221:SF111	WRKY TRANSCRIPTION FACTOR 43-RELATED	coord: 26..184
IPR044810	WRKY transcription factor, plant	PANTHER	PTHR31221	WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATED	coord: 26..184

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g_new0136.1	MC10g_new0136.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0043565	sequence-specific DNA binding