MC10g_new0133 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g_new0133
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	subtilisin-like protease SBT3.16
Location	MC10: 5710429 .. 5711536 (+)
RNA-Seq Expression	MC10g_new0133
Synteny	MC10g_new0133

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGAAATCTCATTTCATTGTTGCATTTGTGTCATTTTATGCATTGTTTTCAATGTTTTCATGTAAAGCCATGGCAGAAGCTGATGACCAGAATCGCAAGGTTCAAATATTACCCATGGAGTTGGCCCATATTCTACATTAAAAAATTTGACCAATTCCATCACTTGATAATTATCTCTTCAGGTTCACATCGTTTACTTGGGAGAAACTCAACACGAAGAGAGTAAATTGACAACTGAGTCTCACCATGAATTACTGTCCACTGTACTGGGAAGGTAATTAATCTACAGCTCAGAATTTTTAATGAAAAAAAAAAAAAAAGGTTTAGTGTTAATTATATTATGATATTTGATAATGAAGCAAGGAGAAGTCGTGGGAATCAATGGTGTACAGCTATAGACATGGCTTTTCTGGGTTTGCAGCCAAGCTTACCAAGTCTCACGCTCAAAAGCTAGCTGGTAAATATACTAATATAAATGTAAACCTAATGAATTAATTTGAGAATAAACTTTCAAAAGATTTCTATTTGGTCCATCTTGTCCCTATATTTAAAAAGATTAGGTAAATTTCTTATGTACTTTTAAGATCATATTGTTCATCTCATTCTATGTTTTAAAAAGTTTGTAATGTGTCTTTAAATTTTTGGATCTTAAAAAAGTTTATGAAAAATTCTTAACTTGAGAAATTGTAGCTTTTTTAGGCTAACTAATCTGAGTTGAATTCAATTTTTGGATAACTAAATTTTTTGAATTCTAATACCATATCAATATTCATAAAATCTGTTTTCAAAATAAGGGAAGAATAACTCATATATATTATAAATCTTTGTTAGGTTTCTTATATTTTCAACCTGTATGCTTAAGAATACTTTTAAAAGTATTATATTAAAATTAATTCAGAAAATTAAAGAAGTAAACTTTTAATTTAAGCCTTCATCACTAAAACAAACAATATGTGGTGGTACGAACGAATTGAACTTCAGAAATGGCGGGCGTGGTTCGAGTTCTTCCAAATTCGATTTACAAAATGCAGACTACAAGAAGTTGGGATTTCCTGGGGCTCTCAACTTCTCCCTCACAATCCTCCAACCTTCTTCATCGCGGTAA

mRNA sequence

ATGGGGAAATCTCATTTCATTGTTGCATTTGTGTCATTTTATGCATTGTTTTCAATGTTTTCATGTAAAGCCATGGCAGAAGCTGATGACCAGAATCGCAAGGTTCACATCGTTTACTTGGGAGAAACTCAACACGAAGAGAGTAAATTGACAACTGAGTCTCACCATGAATTACTGTCCACTGTACTGGGAAGCAAGGAGAAGTCGTGGGAATCAATGGTGTACAGCTATAGACATGGCTTTTCTGGGTTTGCAGCCAAGCTTACCAAGTCTCACGCTCAAAAGCTAGCTGACTACAAGAAGTTGGGATTTCCTGGGGCTCTCAACTTCTCCCTCACAATCCTCCAACCTTCTTCATCGCGGTAA

Coding sequence (CDS)

ATGGGGAAATCTCATTTCATTGTTGCATTTGTGTCATTTTATGCATTGTTTTCAATGTTTTCATGTAAAGCCATGGCAGAAGCTGATGACCAGAATCGCAAGGTTCACATCGTTTACTTGGGAGAAACTCAACACGAAGAGAGTAAATTGACAACTGAGTCTCACCATGAATTACTGTCCACTGTACTGGGAAGCAAGGAGAAGTCGTGGGAATCAATGGTGTACAGCTATAGACATGGCTTTTCTGGGTTTGCAGCCAAGCTTACCAAGTCTCACGCTCAAAAGCTAGCTGACTACAAGAAGTTGGGATTTCCTGGGGCTCTCAACTTCTCCCTCACAATCCTCCAACCTTCTTCATCGCGGTAA

Protein sequence

MGKSHFIVAFVSFYALFSMFSCKAMAEADDQNRKVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGFAAKLTKSHAQKLADYKKLGFPGALNFSLTILQPSSSR

Homology

[Close]

BLAST of MC10g_new0133 vs. ExPASy Swiss-Prot
Match: F4HPF1 (Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 2.7e-21
Identity = 48/65 (73.85%), Postives = 58/65 (89.23%), Query Frame = 0


Query: 34 KVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGFAAKLTKSHA 93
          KVHIVYLGE QH++ K  TESHH++LS++LGSK+ + ESMVYSYRHGFSGFAAKLTKS A
Sbjct: 28 KVHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHESMVYSYRHGFSGFAAKLTKSQA 87

Query: 94 QKLAD 99
          +K+AD
Sbjct: 88 KKIAD 92

[Close]

BLAST of MC10g_new0133 vs. ExPASy Swiss-Prot
Match: Q9MAP7 (Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 6.7e-20
Identity = 48/74 (64.86%), Postives = 62/74 (83.78%), Query Frame = 0


Query: 25 MAEADDQNRKVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGF 84
          +  A D++ KVHIVYLGE QH++ +  +ESHH++LS++LGSK  + ESMVYSYRHGFSGF
Sbjct: 20 VVRASDES-KVHIVYLGEKQHDDPEFVSESHHQMLSSLLGSKVDAHESMVYSYRHGFSGF 79

Query: 85 AAKLTKSHAQKLAD 99
          AAKLT+S A+KLAD
Sbjct: 80 AAKLTESQAKKLAD 92

[Close]

BLAST of MC10g_new0133 vs. ExPASy Swiss-Prot
Match: Q8GWX9 (Subtilisin-like protease SBT3.16 OS=Arabidopsis thaliana OX=3702 GN=SBT3.16 PE=2 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.1e-19
Identity = 46/97 (47.42%), Postives = 69/97 (71.13%), Query Frame = 0


Query: 3   KSHFIVAFVSFYALFSMFSCKAMAEADDQNRKVHIVYLGETQHEESKLTTESHHELLSTV 62
           K HF+V F+    +F +    A+  A ++  +++ V+LGE QH++  + TESHH++L  +
Sbjct: 12  KKHFVVVFIGLVLIFKI----ALITAANEKSQIYTVHLGERQHDDPNIVTESHHDILGPL 71

Query: 63  LGSKEKSWESMVYSYRHGFSGFAAKLTKSHAQKLADY 100
           LGSK+ S ESM+YSYRHGFSGFAAKLT S A++L+ +
Sbjct: 72  LGSKKASHESMIYSYRHGFSGFAAKLTSSQARELSGH 104

[Close]

BLAST of MC10g_new0133 vs. ExPASy Swiss-Prot
Match: Q9SZY3 (Subtilisin-like protease SBT3.8 OS=Arabidopsis thaliana OX=3702 GN=SBT3.8 PE=3 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 3.3e-19
Identity = 51/81 (62.96%), Postives = 61/81 (75.31%), Query Frame = 0


Query: 18 SMFSCKAMAEADDQNRKVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSY 77
          S F   A AE+     KVHIVYLGE QH++ +  TESHH +L ++LGSKE +  SMV+SY
Sbjct: 18 STFVAHAGAES-----KVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDAHSSMVHSY 77

Query: 78 RHGFSGFAAKLTKSHAQKLAD 99
          RHGFSGFAAKLTKS A+KLAD
Sbjct: 78 RHGFSGFAAKLTKSQAKKLAD 93

[Close]

BLAST of MC10g_new0133 vs. ExPASy Swiss-Prot
Match: Q8L7I2 (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 4.4e-19
Identity = 50/94 (53.19%), Postives = 69/94 (73.40%), Query Frame = 0


Query: 5  HFIVAFVSFYALFSMFSCKAMAEADDQNRKVHIVYLGETQHEESKLTTESHHELLSTVLG 64
          + +++ V F  +   F  ++ A+     RKVHIVYLGE QH++ +  TESHH +L ++LG
Sbjct: 9  YVVLSLVIFLNVQRSFVAESSAK-----RKVHIVYLGEKQHDDPEFVTESHHRMLWSLLG 68

Query: 65 SKEKSWESMVYSYRHGFSGFAAKLTKSHAQKLAD 99
          SKE + +SMVYSYRHGFSGFAAKLT+S A+K+AD
Sbjct: 69 SKEDANDSMVYSYRHGFSGFAAKLTESQAKKIAD 97

[Close]

BLAST of MC10g_new0133 vs. NCBI nr
Match: XP_022136924.1 (subtilisin-like protease SBT3.16 [Momordica charantia])

HSP 1 Score: 182 bits (461), Expect = 5.17e-57
Identity = 90/97 (92.78%), Postives = 94/97 (96.91%), Query Frame = 0


Query: 1  MGKSHFIVAFVSFYALFSMFSCKAMAEADDQNRKVHIVYLGETQHEESKLTTESHHELLS 60
          MGKSHFI AFVSFYALFSMFSCK+MAEADDQNRKVHIVYLGETQHE+SKLTTE+HHELL+
Sbjct: 1  MGKSHFIAAFVSFYALFSMFSCKSMAEADDQNRKVHIVYLGETQHEDSKLTTETHHELLA 60

Query: 61 TVLGSKEKSWESMVYSYRHGFSGFAAKLTKSHAQKLA 97
          TVLGSKEKSWESMVYSYRHGFSGFAAKLT S AQKLA
Sbjct: 61 TVLGSKEKSWESMVYSYRHGFSGFAAKLTNSQAQKLA 97

[Close]

BLAST of MC10g_new0133 vs. NCBI nr
Match: XP_022984814.1 (subtilisin-like protease SBT3.9 [Cucurbita maxima])

HSP 1 Score: 126 bits (316), Expect = 8.62e-31
Identity = 68/92 (73.91%), Postives = 76/92 (82.61%), Query Frame = 0


Query: 6  FIVAFVSFYALFSMFSCKAMAEADDQNRKVHIVYLGETQHEESKLTTESHHELLSTVLGS 65
          FI+A V+ YA+FS      MAEADDQN KVHIVYLGE  +++ KLTT+SHHELL +VLGS
Sbjct: 9  FILAVVTIYAVFS-----DMAEADDQNSKVHIVYLGERPYDDVKLTTDSHHELLESVLGS 68

Query: 66 KEKSWESMVYSYRHGFSGFAAKLTKSHAQKLA 97
          KEKS ESMVYSYRHGFSGFAAKLT S AQKLA
Sbjct: 69 KEKSLESMVYSYRHGFSGFAAKLTNSQAQKLA 95

[Close]

BLAST of MC10g_new0133 vs. NCBI nr
Match: KAG6577197.1 (Subtilisin-like protease 3.7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 125 bits (315), Expect = 1.09e-30
Identity = 71/107 (66.36%), Postives = 82/107 (76.64%), Query Frame = 0


Query: 4   SHFIVAFVSFYALFSMFSCKAMAEADDQNRKVHIVYLGETQHEESKLTTESHHELLSTVL 63
           S F+ A V+ YA+FS     AMAEAD+QN KVHIVYLGE  +E+ KLTT+SHHELL +V+
Sbjct: 7   SSFMHAVVTIYAVFS-----AMAEADNQNSKVHIVYLGERPYEDVKLTTDSHHELLESVM 66

Query: 64  GSKEKSWESMVYSYRHGFSGFAAKLTKSHAQKLADYKKLG--FPGAL 108
           GSKEKS ESMVYSYRHGFSGFAAKLT S AQKLA    +   FP +L
Sbjct: 67  GSKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMSIVARVFPSSL 108

[Close]

BLAST of MC10g_new0133 vs. NCBI nr
Match: XP_023552781.1 (subtilisin-like protease SBT3.7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 124 bits (311), Expect = 4.10e-30
Identity = 66/91 (72.53%), Postives = 75/91 (82.42%), Query Frame = 0


Query: 7  IVAFVSFYALFSMFSCKAMAEADDQNRKVHIVYLGETQHEESKLTTESHHELLSTVLGSK 66
          +VA V+ Y +FS     AMAEADDQN KVHIVYLGE  +++ KLTT+SHHELL +VLGSK
Sbjct: 12 VVAVVTIYGVFS-----AMAEADDQNSKVHIVYLGERPYDDVKLTTDSHHELLESVLGSK 71

Query: 67 EKSWESMVYSYRHGFSGFAAKLTKSHAQKLA 97
          EKS ES+VYSYRHGFSGFAAKLT S AQKLA
Sbjct: 72 EKSLESIVYSYRHGFSGFAAKLTNSQAQKLA 97

[Close]

BLAST of MC10g_new0133 vs. NCBI nr
Match: XP_004146562.1 (subtilisin-like protease SBT3.6 [Cucumis sativus] >KGN53334.1 hypothetical protein Csa_014762 [Cucumis sativus])

HSP 1 Score: 124 bits (311), Expect = 4.12e-30
Identity = 63/98 (64.29%), Postives = 76/98 (77.55%), Query Frame = 0


Query: 5   HFIVAFVSFYALFSMFSCKAMAEADDQNRKVHIVYLGETQHEESKLTTESHHELLSTVLG 64
           H IV F+ F  LF       +AEADDQN KVHIVYLGE  H ++K T +SHH+LLST+LG
Sbjct: 9   HLIVGFIIFDCLFK----PILAEADDQNPKVHIVYLGEKPHHDTKFTIDSHHQLLSTILG 68

Query: 65  SKEKSWESMVYSYRHGFSGFAAKLTKSHAQKLADYKKL 102
           SKEKS E+MVYSY+HGFSGFAAKLTKS AQKL++  ++
Sbjct: 69  SKEKSMEAMVYSYKHGFSGFAAKLTKSQAQKLSEMSRV 102

[Close]

BLAST of MC10g_new0133 vs. ExPASy TrEMBL
Match: A0A6J1C5P5 (subtilisin-like protease SBT3.16 OS=Momordica charantia OX=3673 GN=LOC111008501 PE=4 SV=1)

HSP 1 Score: 182 bits (461), Expect = 2.50e-57
Identity = 90/97 (92.78%), Postives = 94/97 (96.91%), Query Frame = 0


Query: 1  MGKSHFIVAFVSFYALFSMFSCKAMAEADDQNRKVHIVYLGETQHEESKLTTESHHELLS 60
          MGKSHFI AFVSFYALFSMFSCK+MAEADDQNRKVHIVYLGETQHE+SKLTTE+HHELL+
Sbjct: 1  MGKSHFIAAFVSFYALFSMFSCKSMAEADDQNRKVHIVYLGETQHEDSKLTTETHHELLA 60

Query: 61 TVLGSKEKSWESMVYSYRHGFSGFAAKLTKSHAQKLA 97
          TVLGSKEKSWESMVYSYRHGFSGFAAKLT S AQKLA
Sbjct: 61 TVLGSKEKSWESMVYSYRHGFSGFAAKLTNSQAQKLA 97

[Close]

BLAST of MC10g_new0133 vs. ExPASy TrEMBL
Match: A0A6J1JBL4 (subtilisin-like protease SBT3.9 OS=Cucurbita maxima OX=3661 GN=LOC111482985 PE=3 SV=1)

HSP 1 Score: 126 bits (316), Expect = 4.17e-31
Identity = 68/92 (73.91%), Postives = 76/92 (82.61%), Query Frame = 0


Query: 6  FIVAFVSFYALFSMFSCKAMAEADDQNRKVHIVYLGETQHEESKLTTESHHELLSTVLGS 65
          FI+A V+ YA+FS      MAEADDQN KVHIVYLGE  +++ KLTT+SHHELL +VLGS
Sbjct: 9  FILAVVTIYAVFS-----DMAEADDQNSKVHIVYLGERPYDDVKLTTDSHHELLESVLGS 68

Query: 66 KEKSWESMVYSYRHGFSGFAAKLTKSHAQKLA 97
          KEKS ESMVYSYRHGFSGFAAKLT S AQKLA
Sbjct: 69 KEKSLESMVYSYRHGFSGFAAKLTNSQAQKLA 95

[Close]

BLAST of MC10g_new0133 vs. ExPASy TrEMBL
Match: A0A0A0KWS6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G047950 PE=3 SV=1)

HSP 1 Score: 124 bits (311), Expect = 2.00e-30
Identity = 63/98 (64.29%), Postives = 76/98 (77.55%), Query Frame = 0


Query: 5   HFIVAFVSFYALFSMFSCKAMAEADDQNRKVHIVYLGETQHEESKLTTESHHELLSTVLG 64
           H IV F+ F  LF       +AEADDQN KVHIVYLGE  H ++K T +SHH+LLST+LG
Sbjct: 9   HLIVGFIIFDCLFK----PILAEADDQNPKVHIVYLGEKPHHDTKFTIDSHHQLLSTILG 68

Query: 65  SKEKSWESMVYSYRHGFSGFAAKLTKSHAQKLADYKKL 102
           SKEKS E+MVYSY+HGFSGFAAKLTKS AQKL++  ++
Sbjct: 69  SKEKSMEAMVYSYKHGFSGFAAKLTKSQAQKLSEMSRV 102

[Close]

BLAST of MC10g_new0133 vs. ExPASy TrEMBL
Match: A0A5A7TRR2 (Subtilisin-like protease SBT3.7 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G001180 PE=3 SV=1)

HSP 1 Score: 121 bits (304), Expect = 1.75e-29
Identity = 61/100 (61.00%), Postives = 79/100 (79.00%), Query Frame = 0


Query: 4   SHFIVAFVSFYALFSMFSCKA-MAEADDQNRKVHIVYLGETQHEESKLTTESHHELLSTV 63
           +H IV F+ FY L     C+  +AE++DQN KVHIVYLGE  H ++K TT+SHH+LL+ +
Sbjct: 8   AHLIVGFIIFYGL-----CEPILAESNDQNPKVHIVYLGEKPHHDTKFTTDSHHQLLAAI 67

Query: 64  LGSKEKSWESMVYSYRHGFSGFAAKLTKSHAQKLADYKKL 102
           LGSKEKS E+MVYSY+HGFSGFAAKLTKS AQKL++  ++
Sbjct: 68  LGSKEKSLEAMVYSYKHGFSGFAAKLTKSEAQKLSEMSRV 102

[Close]

BLAST of MC10g_new0133 vs. ExPASy TrEMBL
Match: A0A1S3BU23 (subtilisin-like protease SBT3.7 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493167 PE=3 SV=1)

HSP 1 Score: 121 bits (304), Expect = 1.76e-29
Identity = 61/100 (61.00%), Postives = 79/100 (79.00%), Query Frame = 0


Query: 4   SHFIVAFVSFYALFSMFSCKA-MAEADDQNRKVHIVYLGETQHEESKLTTESHHELLSTV 63
           +H IV F+ FY L     C+  +AE++DQN KVHIVYLGE  H ++K TT+SHH+LL+ +
Sbjct: 8   AHLIVGFIIFYGL-----CEPILAESNDQNPKVHIVYLGEKPHHDTKFTTDSHHQLLAAI 67

Query: 64  LGSKEKSWESMVYSYRHGFSGFAAKLTKSHAQKLADYKKL 102
           LGSKEKS E+MVYSY+HGFSGFAAKLTKS AQKL++  ++
Sbjct: 68  LGSKEKSLEAMVYSYKHGFSGFAAKLTKSEAQKLSEMSRV 102

[Close]

BLAST of MC10g_new0133 vs. TAIR 10
Match: AT1G32950.1 (Subtilase family protein )

HSP 1 Score: 102.8 bits (255), Expect = 1.9e-22
Identity = 48/65 (73.85%), Postives = 58/65 (89.23%), Query Frame = 0


Query: 34 KVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGFAAKLTKSHA 93
          KVHIVYLGE QH++ K  TESHH++LS++LGSK+ + ESMVYSYRHGFSGFAAKLTKS A
Sbjct: 28 KVHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHESMVYSYRHGFSGFAAKLTKSQA 87

Query: 94 QKLAD 99
          +K+AD
Sbjct: 88 KKIAD 92

[Close]

BLAST of MC10g_new0133 vs. TAIR 10
Match: AT1G32940.1 (Subtilase family protein )

HSP 1 Score: 98.2 bits (243), Expect = 4.8e-21
Identity = 48/74 (64.86%), Postives = 62/74 (83.78%), Query Frame = 0


Query: 25 MAEADDQNRKVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGF 84
          +  A D++ KVHIVYLGE QH++ +  +ESHH++LS++LGSK  + ESMVYSYRHGFSGF
Sbjct: 20 VVRASDES-KVHIVYLGEKQHDDPEFVSESHHQMLSSLLGSKVDAHESMVYSYRHGFSGF 79

Query: 85 AAKLTKSHAQKLAD 99
          AAKLT+S A+KLAD
Sbjct: 80 AAKLTESQAKKLAD 92

[Close]

BLAST of MC10g_new0133 vs. TAIR 10
Match: AT1G66210.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 97.4 bits (241), Expect = 8.1e-21
Identity = 46/97 (47.42%), Postives = 69/97 (71.13%), Query Frame = 0


Query: 3   KSHFIVAFVSFYALFSMFSCKAMAEADDQNRKVHIVYLGETQHEESKLTTESHHELLSTV 62
           K HF+V F+    +F +    A+  A ++  +++ V+LGE QH++  + TESHH++L  +
Sbjct: 12  KKHFVVVFIGLVLIFKI----ALITAANEKSQIYTVHLGERQHDDPNIVTESHHDILGPL 71

Query: 63  LGSKEKSWESMVYSYRHGFSGFAAKLTKSHAQKLADY 100
           LGSK+ S ESM+YSYRHGFSGFAAKLT S A++L+ +
Sbjct: 72  LGSKKASHESMIYSYRHGFSGFAAKLTSSQARELSGH 104

[Close]

BLAST of MC10g_new0133 vs. TAIR 10
Match: AT4G10550.3 (Subtilase family protein )

HSP 1 Score: 97.4 bits (241), Expect = 8.1e-21
Identity = 46/66 (69.70%), Postives = 57/66 (86.36%), Query Frame = 0


Query: 33  RKVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGFAAKLTKSH 92
           RKVHIVYLGE QH++ +  TESHH +L ++LGSKE + +SMVYSYRHGFSGFAAKLT+S 
Sbjct: 48  RKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAAKLTESQ 107

Query: 93  AQKLAD 99
           A+K+AD
Sbjct: 108 AKKIAD 113

[Close]

BLAST of MC10g_new0133 vs. TAIR 10
Match: AT4G10540.1 (Subtilase family protein )

HSP 1 Score: 95.9 bits (237), Expect = 2.4e-20
Identity = 51/81 (62.96%), Postives = 61/81 (75.31%), Query Frame = 0


Query: 18 SMFSCKAMAEADDQNRKVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSY 77
          S F   A AE+     KVHIVYLGE QH++ +  TESHH +L ++LGSKE +  SMV+SY
Sbjct: 18 STFVAHAGAES-----KVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDAHSSMVHSY 77

Query: 78 RHGFSGFAAKLTKSHAQKLAD 99
          RHGFSGFAAKLTKS A+KLAD
Sbjct: 78 RHGFSGFAAKLTKSQAKKLAD 93

The following BLAST results are available for this feature:

BLAST of MC10g_new0133 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4HPF1	2.7e-21	73.85	Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 S...	[more]
Q9MAP7	6.7e-20	64.86	Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 S...	[more]
Q8GWX9	1.1e-19	47.42	Subtilisin-like protease SBT3.16 OS=Arabidopsis thaliana OX=3702 GN=SBT3.16 PE=2...	[more]
Q9SZY3	3.3e-19	62.96	Subtilisin-like protease SBT3.8 OS=Arabidopsis thaliana OX=3702 GN=SBT3.8 PE=3 S...	[more]
Q8L7I2	4.4e-19	53.19	Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 S...	[more]

BLAST of MC10g_new0133 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022136924.1	5.17e-57	92.78	subtilisin-like protease SBT3.16 [Momordica charantia]	[more]
XP_022984814.1	8.62e-31	73.91	subtilisin-like protease SBT3.9 [Cucurbita maxima]	[more]
KAG6577197.1	1.09e-30	66.36	Subtilisin-like protease 3.7, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023552781.1	4.10e-30	72.53	subtilisin-like protease SBT3.7 [Cucurbita pepo subsp. pepo]	[more]
XP_004146562.1	4.12e-30	64.29	subtilisin-like protease SBT3.6 [Cucumis sativus] >KGN53334.1 hypothetical prote...	[more]

BLAST of MC10g_new0133 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C5P5	2.50e-57	92.78	subtilisin-like protease SBT3.16 OS=Momordica charantia OX=3673 GN=LOC111008501 ...	[more]
A0A6J1JBL4	4.17e-31	73.91	subtilisin-like protease SBT3.9 OS=Cucurbita maxima OX=3661 GN=LOC111482985 PE=3...	[more]
A0A0A0KWS6	2.00e-30	64.29	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G047950 PE=3 SV=1	[more]
A0A5A7TRR2	1.75e-29	61.00	Subtilisin-like protease SBT3.7 isoform X1 OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3BU23	1.76e-29	61.00	subtilisin-like protease SBT3.7 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034931...	[more]

BLAST of MC10g_new0133 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G32950.1	1.9e-22	73.85	Subtilase family protein	[more]
AT1G32940.1	4.8e-21	64.86	Subtilase family protein	[more]
AT1G66210.1	8.1e-21	47.42	Subtilisin-like serine endopeptidase family protein	[more]
AT4G10550.3	8.1e-21	69.70	Subtilase family protein	[more]
AT4G10540.1	2.4e-20	62.96	Subtilase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR037045	Peptidase S8 propeptide/proteinase inhibitor I9 superfamily	GENE3D	3.30.70.80	Peptidase S8 propeptide/proteinase inhibitor I9	coord: 18..105 e-value: 6.2E-22 score: 79.9
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 35..98 e-value: 8.6E-11 score: 42.4
None	No IPR available	PANTHER	PTHR43806:SF50	SUBTILISIN-LIKE PROTEASE SBT3.1-RELATED	coord: 24..99
None	No IPR available	PANTHER	PTHR43806	PEPTIDASE S8	coord: 24..99
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..22 score: 5.0

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g_new0133.1	MC10g_new0133.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005615	extracellular space
molecular_function	GO:0004252	serine-type endopeptidase activity