MC10g_new0112 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g_new0112
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Cytochrome P450
Location	MC10: 4330516 .. 4330989 (-)
RNA-Seq Expression	MC10g_new0112
Synteny	MC10g_new0112

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTCGTCGGAAGCCGTTGCCGGAATCTTCGCCCTTCTCCTCTTTCTCTATGCCCTCTTCCGGGCATCTCCAATATCCGGTGCTCACCGGAAGAGACTCCCACCCCAACCCGCCGGCGCTTGGCCGCTGATCGGCCACCTCCATCTACTAAGTTCAACAGAACCGGCCCACATAACGTTCGCCAAAATGGCAGACGCCTACGGACCAATCTTCACGCTAAAATTGGGTATGAACAGAGTCTTGATAGTAAGCAGTTGGGAAGTAGCGAGAGAGTGCTTCACCACCAACGACAGAGTATTTTCCTCCCGTCCAAAAATGGTGGCCTCGAAGCTTCTGGGCTACGACCACGCCATGATGGGGTTAAGCCCCTACGGCCCACACTGGCGCCACATGCGCAAAATCGCCACGCTTCAACTCCTCACCAACCACCGCCTCGAGCAGCTACAACACATCATAGTATCCGAGGTCTAA

mRNA sequence

ATGGCCTCGTCGGAAGCCGTTGCCGGAATCTTCGCCCTTCTCCTCTTTCTCTATGCCCTCTTCCGGGCATCTCCAATATCCGGTGCTCACCGGAAGAGACTCCCACCCCAACCCGCCGGCGCTTGGCCGCTGATCGGCCACCTCCATCTACTAAGTTCAACAGAACCGGCCCACATAACGTTCGCCAAAATGGCAGACGCCTACGGACCAATCTTCACGCTAAAATTGGGTATGAACAGAGTCTTGATAGTAAGCAGTTGGGAAGTAGCGAGAGAGTGCTTCACCACCAACGACAGAGTATTTTCCTCCCGTCCAAAAATGGTGGCCTCGAAGCTTCTGGGCTACGACCACGCCATGATGGGGTTAAGCCCCTACGGCCCACACTGGCGCCACATGCGCAAAATCGCCACGCTTCAACTCCTCACCAACCACCGCCTCGAGCAGCTACAACACATCATAGTATCCGAGGTCTAA

Coding sequence (CDS)

ATGGCCTCGTCGGAAGCCGTTGCCGGAATCTTCGCCCTTCTCCTCTTTCTCTATGCCCTCTTCCGGGCATCTCCAATATCCGGTGCTCACCGGAAGAGACTCCCACCCCAACCCGCCGGCGCTTGGCCGCTGATCGGCCACCTCCATCTACTAAGTTCAACAGAACCGGCCCACATAACGTTCGCCAAAATGGCAGACGCCTACGGACCAATCTTCACGCTAAAATTGGGTATGAACAGAGTCTTGATAGTAAGCAGTTGGGAAGTAGCGAGAGAGTGCTTCACCACCAACGACAGAGTATTTTCCTCCCGTCCAAAAATGGTGGCCTCGAAGCTTCTGGGCTACGACCACGCCATGATGGGGTTAAGCCCCTACGGCCCACACTGGCGCCACATGCGCAAAATCGCCACGCTTCAACTCCTCACCAACCACCGCCTCGAGCAGCTACAACACATCATAGTATCCGAGGTCTAA

Protein sequence

MASSEAVAGIFALLLFLYALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHITFAKMADAYGPIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMGLSPYGPHWRHMRKIATLQLLTNHRLEQLQHIIVSEV

Homology

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BLAST of MC10g_new0112 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 157.1 bits (396), Expect = 1.6e-37
Identity = 76/146 (52.05%), Postives = 104/146 (71.23%), Query Frame = 0


Query: 12  ALLLFLYALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHITFAKMADAYGPI 71
           +L+  L+ LFR S +S  H K  PP  +G+WPL+GHL L+ +T+  H T   + D YGPI
Sbjct: 15  SLISLLFFLFRFSKVS--HTKE-PPIISGSWPLLGHLPLMRNTQTPHKTLGALVDKYGPI 74

Query: 72  FTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMGLSPYGPHWRH 131
           FT+KLG    L++S+WE+A+ECFT ND V SSRPK VA +L+ Y+ A +G +PYG +WR 
Sbjct: 75  FTIKLGATNALVLSNWELAKECFTKNDIVVSSRPKPVAVELMSYNQAFIGWAPYGAYWRQ 134

Query: 132 MRKIATLQLLTNHRLEQLQHIIVSEV 158
           +RKI TL++L+N R+E L HI VSEV
Sbjct: 135 LRKIVTLEILSNRRIELLSHIRVSEV 157

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BLAST of MC10g_new0112 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 1.3e-36
Identity = 71/150 (47.33%), Postives = 108/150 (72.00%), Query Frame = 0


Query: 9   GIFALL-LFLYALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHITFAKMADA 68
           G F LL  F Y +++       ++ + PPQPAGAWP+IGHLHLL+  +  H   +  AD 
Sbjct: 13  GFFVLLPCFFYFVWK----KPNNKIKEPPQPAGAWPIIGHLHLLARGDLPHKILSSFADK 72

Query: 69  YGPIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMGLSPYGP 128
            GP+F ++LG+++ L+V++ E+A+ECFTTNDR F +RP  VA+K++GY++ M+G++PYGP
Sbjct: 73  NGPVFKIQLGVHQALVVNNSEIAKECFTTNDRFFLNRPSGVAAKIMGYNYVMLGVAPYGP 132

Query: 129 HWRHMRKIATLQLLTNHRLEQLQHIIVSEV 158
           +WR MRKI  L+ L+N RL+ L+H+  SE+
Sbjct: 133 YWRDMRKIIMLEFLSNRRLQSLKHVWHSEI 158

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BLAST of MC10g_new0112 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 5.1e-36
Identity = 71/152 (46.71%), Postives = 104/152 (68.42%), Query Frame = 0


Query: 6   AVAGIFALLLFLYALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHITFAKMA 65
           A+A I +L+     L+R +      R +  P  +GAWP++GHL LL+ ++  H     +A
Sbjct: 14  AIASILSLIFLCLFLYRKN-----SRGKDAPVVSGAWPILGHLSLLNGSQTPHKVLGALA 73

Query: 66  DAYGPIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMGLSPY 125
           D YGP+FT+KLGM   L++S+WE+++E FTTND   SSRPK+VA +++ Y+ A +GL+PY
Sbjct: 74  DKYGPLFTIKLGMKPALVLSNWEMSKELFTTNDLAVSSRPKLVAVEVMSYNQAFVGLAPY 133

Query: 126 GPHWRHMRKIATLQLLTNHRLEQLQHIIVSEV 158
           GP+WR +RKI T + L+N R+EQ  HI VSEV
Sbjct: 134 GPYWRELRKIVTFEFLSNRRIEQRNHIRVSEV 160

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BLAST of MC10g_new0112 vs. ExPASy Swiss-Prot
Match: O49394 (Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=1 SV=2)

HSP 1 Score: 148.3 bits (373), Expect = 7.3e-35
Identity = 76/147 (51.70%), Postives = 103/147 (70.07%), Query Frame = 0


Query: 13  LLLFLY-ALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEP-AHITFAKMADAYGP 72
           +L+F++ ALF+ S     H K   P P+GAWP+IGHLHLLS  E   + T  KMAD YGP
Sbjct: 12  ILVFVFIALFKKSK-KPKHVK--APAPSGAWPIIGHLHLLSGKEQLLYRTLGKMADQYGP 71

Query: 73  IFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMGLSPYGPHWR 132
             +L+LG +   +VSS+EVA++CFT ND+  +SRP   A+K +GYD A+ G +PY   WR
Sbjct: 72  AMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFAPYSAFWR 131

Query: 133 HMRKIATLQLLTNHRLEQLQHIIVSEV 158
            MRKIATL+LL+N RL+ L+H+ VSE+
Sbjct: 132 EMRKIATLELLSNRRLQMLKHVRVSEI 155

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BLAST of MC10g_new0112 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 1.6e-34
Identity = 67/145 (46.21%), Postives = 100/145 (68.97%), Query Frame = 0


Query: 14  LLFLYALFRASPISGAH-RKRLPPQPAGAWPLIGHLHLLSSTEPAHITFAKMADAYGPIF 73
           L+FL  LF   P+   H   +  P   GAWP+ GHL LL  ++  H     +A+ +GP+F
Sbjct: 18  LIFLLCLFLYGPLKKVHGSSKEAPTVGGAWPIFGHLPLLIGSKSPHKALGALAEKHGPLF 77

Query: 74  TLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMGLSPYGPHWRHM 133
           T+KLG  + L+VS WE+ARECFTTND   S+RPK++ ++L+ Y++AM+ ++PYGP+WR +
Sbjct: 78  TIKLGAKKALVVSDWEMARECFTTNDVAVSARPKLLVAELMCYNNAMLLVAPYGPYWREL 137

Query: 134 RKIATLQLLTNHRLEQLQHIIVSEV 158
           RKI   ++L++ R+EQLQ + VSEV
Sbjct: 138 RKIIVTEILSSSRVEQLQDVRVSEV 162

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BLAST of MC10g_new0112 vs. NCBI nr
Match: XP_022982223.1 (cytochrome P450 CYP82D47-like [Cucurbita maxima])

HSP 1 Score: 254 bits (648), Expect = 2.54e-79
Identity = 121/156 (77.56%), Postives = 140/156 (89.74%), Query Frame = 0


Query: 2   ASSEAVAGIFALLLFLYALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHITF 61
           +SS AVAGIF LL+FLYALF  S    ++ KRLPP+PAGAWP+IGHLHLLS+TEPAHIT 
Sbjct: 10  SSSAAVAGIFTLLIFLYALFNISTKLSSNPKRLPPEPAGAWPVIGHLHLLSTTEPAHITL 69

Query: 62  AKMADAYGPIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMG 121
           AKMAD YGPIFTLKLGM+R LIVSSWE+ARECFTT DR+F+SRPK+VASKLLGY++AMMG
Sbjct: 70  AKMADVYGPIFTLKLGMHRTLIVSSWEIARECFTTKDRIFASRPKLVASKLLGYNYAMMG 129

Query: 122 LSPYGPHWRHMRKIATLQLLTNHRLEQLQHIIVSEV 157
           LSPYG HWRH+RK+ATL+L TNHRL+QLQH+ VSEV
Sbjct: 130 LSPYGSHWRHVRKMATLELFTNHRLQQLQHVRVSEV 165

[Close]

BLAST of MC10g_new0112 vs. NCBI nr
Match: XP_022940489.1 (cytochrome P450 CYP82D47-like [Cucurbita moschata])

HSP 1 Score: 251 bits (642), Expect = 1.86e-78
Identity = 119/156 (76.28%), Postives = 140/156 (89.74%), Query Frame = 0


Query: 2   ASSEAVAGIFALLLFLYALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHITF 61
           +SS AVAGIFALL+FLY LF  S    ++ KRLPP+PAGAWP+IGHLHLLS+TEPAHIT 
Sbjct: 7   SSSAAVAGIFALLIFLYVLFNISKRLSSNPKRLPPEPAGAWPVIGHLHLLSTTEPAHITL 66

Query: 62  AKMADAYGPIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMG 121
           AKMAD YGPIFTL+LGM++ LIVSSWE+ARECFTT DR+F+SRPK+VASKLLGY++AMMG
Sbjct: 67  AKMADVYGPIFTLRLGMHKALIVSSWEIARECFTTKDRIFASRPKLVASKLLGYNYAMMG 126

Query: 122 LSPYGPHWRHMRKIATLQLLTNHRLEQLQHIIVSEV 157
           LSPYG HWRH+RK+ATL+L TNHRL+QLQH+ VSEV
Sbjct: 127 LSPYGSHWRHVRKMATLELFTNHRLQQLQHVRVSEV 162

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BLAST of MC10g_new0112 vs. NCBI nr
Match: XP_023523452.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 251 bits (640), Expect = 3.71e-78
Identity = 119/156 (76.28%), Postives = 140/156 (89.74%), Query Frame = 0


Query: 2   ASSEAVAGIFALLLFLYALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHITF 61
           +SS AVAGIFALL+FLYALF       ++ KRLPP+PAGAWP+IGHLHLLS+TEPAHIT 
Sbjct: 7   SSSAAVAGIFALLIFLYALFNIYKRLSSNPKRLPPEPAGAWPVIGHLHLLSTTEPAHITL 66

Query: 62  AKMADAYGPIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMG 121
           AKMAD YGPIFTL+LGM++ LIVSSWE+ARECFTT DR+F+SRPK+VASKLLGY++AMMG
Sbjct: 67  AKMADVYGPIFTLRLGMHKALIVSSWEIARECFTTKDRIFASRPKLVASKLLGYNYAMMG 126

Query: 122 LSPYGPHWRHMRKIATLQLLTNHRLEQLQHIIVSEV 157
           LSPYG HWRH+RK+ATL+L TNHRL+QLQH+ VSEV
Sbjct: 127 LSPYGSHWRHVRKMATLELFTNHRLQQLQHVRVSEV 162

[Close]

BLAST of MC10g_new0112 vs. NCBI nr
Match: XP_038896981.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 244 bits (624), Expect = 1.11e-75
Identity = 113/157 (71.97%), Postives = 137/157 (87.26%), Query Frame = 0


Query: 2   ASSEAVAGIFALLLFLYALFRASP-ISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHIT 61
           +S+  +AGIF+++LFLYALF  S  + GAHR +LPP+P G WPLIGHLHLL++ EPAHIT
Sbjct: 8   SSATVIAGIFSVVLFLYALFIISTRVIGAHRNKLPPEPVGGWPLIGHLHLLNAAEPAHIT 67

Query: 62  FAKMADAYGPIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMM 121
           FAK+AD YGPIFTLKLGM + LIVSSWE+A ECFTTND++F+SRPK+VASKLLGYD+AM 
Sbjct: 68  FAKLADTYGPIFTLKLGMKKALIVSSWEIANECFTTNDKIFASRPKLVASKLLGYDYAMF 127

Query: 122 GLSPYGPHWRHMRKIATLQLLTNHRLEQLQHIIVSEV 157
           G SPYGPHWRH+RK+AT++LLTNHRL+QLQHI   EV
Sbjct: 128 GFSPYGPHWRHVRKLATVELLTNHRLDQLQHIRAFEV 164

[Close]

BLAST of MC10g_new0112 vs. NCBI nr
Match: XP_008464766.1 (PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo] >KAA0032728.1 cytochrome P450 CYP82D47-like [Cucumis melo var. makuwa] >TYJ98760.1 cytochrome P450 CYP82D47-like [Cucumis melo var. makuwa])

HSP 1 Score: 240 bits (612), Expect = 6.28e-74
Identity = 113/156 (72.44%), Postives = 136/156 (87.18%), Query Frame = 0


Query: 3   SSEAVAGIFALLLFLYALFRASP-ISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHITF 62
           S+  V GIF+LLLFLY LF  S  + GAHRK+LPP+PAG WP+IGHLHLLS+TEPAHIT 
Sbjct: 9   SATVVTGIFSLLLFLYTLFNISTRVVGAHRKKLPPEPAGHWPVIGHLHLLSATEPAHITL 68

Query: 63  AKMADAYGPIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMG 122
           AK+AD YGPIFTLKLGM++ L+VSSWE+A+ECFTTNDR+F+SRPK+VASKLLGY++ M+G
Sbjct: 69  AKLADTYGPIFTLKLGMSKALVVSSWEIAKECFTTNDRIFASRPKLVASKLLGYNYNMLG 128

Query: 123 LSPYGPHWRHMRKIATLQLLTNHRLEQLQHIIVSEV 157
           LSPYGP+WRH+RK+ATL+LLTN RL Q QHI   EV
Sbjct: 129 LSPYGPYWRHIRKLATLELLTNRRLHQFQHIREFEV 164

[Close]

BLAST of MC10g_new0112 vs. ExPASy TrEMBL
Match: A0A6J1J4B0 (cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111481116 PE=3 SV=1)

HSP 1 Score: 254 bits (648), Expect = 1.23e-79
Identity = 121/156 (77.56%), Postives = 140/156 (89.74%), Query Frame = 0


Query: 2   ASSEAVAGIFALLLFLYALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHITF 61
           +SS AVAGIF LL+FLYALF  S    ++ KRLPP+PAGAWP+IGHLHLLS+TEPAHIT 
Sbjct: 10  SSSAAVAGIFTLLIFLYALFNISTKLSSNPKRLPPEPAGAWPVIGHLHLLSTTEPAHITL 69

Query: 62  AKMADAYGPIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMG 121
           AKMAD YGPIFTLKLGM+R LIVSSWE+ARECFTT DR+F+SRPK+VASKLLGY++AMMG
Sbjct: 70  AKMADVYGPIFTLKLGMHRTLIVSSWEIARECFTTKDRIFASRPKLVASKLLGYNYAMMG 129

Query: 122 LSPYGPHWRHMRKIATLQLLTNHRLEQLQHIIVSEV 157
           LSPYG HWRH+RK+ATL+L TNHRL+QLQH+ VSEV
Sbjct: 130 LSPYGSHWRHVRKMATLELFTNHRLQQLQHVRVSEV 165

[Close]

BLAST of MC10g_new0112 vs. ExPASy TrEMBL
Match: A0A6J1FKD2 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111446070 PE=3 SV=1)

HSP 1 Score: 251 bits (642), Expect = 9.01e-79
Identity = 119/156 (76.28%), Postives = 140/156 (89.74%), Query Frame = 0


Query: 2   ASSEAVAGIFALLLFLYALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHITF 61
           +SS AVAGIFALL+FLY LF  S    ++ KRLPP+PAGAWP+IGHLHLLS+TEPAHIT 
Sbjct: 7   SSSAAVAGIFALLIFLYVLFNISKRLSSNPKRLPPEPAGAWPVIGHLHLLSTTEPAHITL 66

Query: 62  AKMADAYGPIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMG 121
           AKMAD YGPIFTL+LGM++ LIVSSWE+ARECFTT DR+F+SRPK+VASKLLGY++AMMG
Sbjct: 67  AKMADVYGPIFTLRLGMHKALIVSSWEIARECFTTKDRIFASRPKLVASKLLGYNYAMMG 126

Query: 122 LSPYGPHWRHMRKIATLQLLTNHRLEQLQHIIVSEV 157
           LSPYG HWRH+RK+ATL+L TNHRL+QLQH+ VSEV
Sbjct: 127 LSPYGSHWRHVRKMATLELFTNHRLQQLQHVRVSEV 162

[Close]

BLAST of MC10g_new0112 vs. ExPASy TrEMBL
Match: A0A5D3BG48 (Cytochrome P450 CYP82D47-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold156G00680 PE=3 SV=1)

HSP 1 Score: 240 bits (612), Expect = 3.04e-74
Identity = 113/156 (72.44%), Postives = 136/156 (87.18%), Query Frame = 0


Query: 3   SSEAVAGIFALLLFLYALFRASP-ISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHITF 62
           S+  V GIF+LLLFLY LF  S  + GAHRK+LPP+PAG WP+IGHLHLLS+TEPAHIT 
Sbjct: 9   SATVVTGIFSLLLFLYTLFNISTRVVGAHRKKLPPEPAGHWPVIGHLHLLSATEPAHITL 68

Query: 63  AKMADAYGPIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMG 122
           AK+AD YGPIFTLKLGM++ L+VSSWE+A+ECFTTNDR+F+SRPK+VASKLLGY++ M+G
Sbjct: 69  AKLADTYGPIFTLKLGMSKALVVSSWEIAKECFTTNDRIFASRPKLVASKLLGYNYNMLG 128

Query: 123 LSPYGPHWRHMRKIATLQLLTNHRLEQLQHIIVSEV 157
           LSPYGP+WRH+RK+ATL+LLTN RL Q QHI   EV
Sbjct: 129 LSPYGPYWRHIRKLATLELLTNRRLHQFQHIREFEV 164

[Close]

BLAST of MC10g_new0112 vs. ExPASy TrEMBL
Match: A0A1S3CMB9 (cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502574 PE=3 SV=1)

HSP 1 Score: 240 bits (612), Expect = 3.04e-74
Identity = 113/156 (72.44%), Postives = 136/156 (87.18%), Query Frame = 0


Query: 3   SSEAVAGIFALLLFLYALFRASP-ISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHITF 62
           S+  V GIF+LLLFLY LF  S  + GAHRK+LPP+PAG WP+IGHLHLLS+TEPAHIT 
Sbjct: 9   SATVVTGIFSLLLFLYTLFNISTRVVGAHRKKLPPEPAGHWPVIGHLHLLSATEPAHITL 68

Query: 63  AKMADAYGPIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMG 122
           AK+AD YGPIFTLKLGM++ L+VSSWE+A+ECFTTNDR+F+SRPK+VASKLLGY++ M+G
Sbjct: 69  AKLADTYGPIFTLKLGMSKALVVSSWEIAKECFTTNDRIFASRPKLVASKLLGYNYNMLG 128

Query: 123 LSPYGPHWRHMRKIATLQLLTNHRLEQLQHIIVSEV 157
           LSPYGP+WRH+RK+ATL+LLTN RL Q QHI   EV
Sbjct: 129 LSPYGPYWRHIRKLATLELLTNRRLHQFQHIREFEV 164

[Close]

BLAST of MC10g_new0112 vs. ExPASy TrEMBL
Match: A0A5A7SU10 (Cytochrome P450 CYP82D47-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold156G00630 PE=3 SV=1)

HSP 1 Score: 233 bits (594), Expect = 1.55e-71
Identity = 112/158 (70.89%), Postives = 135/158 (85.44%), Query Frame = 0


Query: 2   ASSEAVAGIFALLLFLYALFRA--SPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHI 61
           +S + V GIFAL++FLYALF+     +   HRKRLPP+  GA PLIGHLHLL+  EPAH 
Sbjct: 7   SSPDTVTGIFALIIFLYALFKIYRGGVGARHRKRLPPEVGGALPLIGHLHLLAKNEPAHK 66

Query: 62  TFAKMADAYGPIFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAM 121
           TFAKMADAYGPIFTL+LG+   LIVS+WE+ARECFTTND++F+SRPK+VASKLLGYD+AM
Sbjct: 67  TFAKMADAYGPIFTLRLGLYTNLIVSNWEIARECFTTNDKIFASRPKLVASKLLGYDYAM 126

Query: 122 MGLSPYGPHWRHMRKIATLQLLTNHRLEQLQHIIVSEV 157
            GLSPYGPHWRH+RK+A L+LL+N+RLE+LQHI VSEV
Sbjct: 127 FGLSPYGPHWRHVRKLAMLELLSNYRLEKLQHIRVSEV 164

[Close]

BLAST of MC10g_new0112 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 148.3 bits (373), Expect = 5.2e-36
Identity = 76/147 (51.70%), Postives = 103/147 (70.07%), Query Frame = 0


Query: 13  LLLFLY-ALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEP-AHITFAKMADAYGP 72
           +L+F++ ALF+ S     H K   P P+GAWP+IGHLHLLS  E   + T  KMAD YGP
Sbjct: 12  ILVFVFIALFKKSK-KPKHVK--APAPSGAWPIIGHLHLLSGKEQLLYRTLGKMADQYGP 71

Query: 73  IFTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMGLSPYGPHWR 132
             +L+LG +   +VSS+EVA++CFT ND+  +SRP   A+K +GYD A+ G +PY   WR
Sbjct: 72  AMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFAPYSAFWR 131

Query: 133 HMRKIATLQLLTNHRLEQLQHIIVSEV 158
            MRKIATL+LL+N RL+ L+H+ VSE+
Sbjct: 132 EMRKIATLELLSNRRLQMLKHVRVSEI 155

[Close]

BLAST of MC10g_new0112 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 145.6 bits (366), Expect = 3.4e-35
Identity = 66/123 (53.66%), Postives = 89/123 (72.36%), Query Frame = 0


Query: 36  PQPAGAWPLIGHLHLLSSTEP-AHITFAKMADAYGPIFTLKLGMNRVLIVSSWEVARECF 95
           P P+GAWP+IGHLHLL   E   + T  KMAD YGP  +L+LG N   +VSS+EVA++CF
Sbjct: 33  PAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSLQLGSNEAFVVSSFEVAKDCF 92

Query: 96  TTNDRVFSSRPKMVASKLLGYDHAMMGLSPYGPHWRHMRKIATLQLLTNHRLEQLQHIIV 155
           T ND+  +SRP   A+K +GY+ A+ G +PY   WR MRKIAT++LL+N RL+ L+H+ V
Sbjct: 93  TVNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWREMRKIATIELLSNRRLQMLKHVRV 152

Query: 156 SEV 158
           SE+
Sbjct: 153 SEI 155

[Close]

BLAST of MC10g_new0112 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 128.3 bits (321), Expect = 5.6e-30
Identity = 59/131 (45.04%), Postives = 86/131 (65.65%), Query Frame = 0


Query: 12  ALLLFLYALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHITFAKMADAYGPI 71
           AL +F   +   S +   ++K   P   GAWPL+GHLHL  +  P H+TF  MAD YGP+
Sbjct: 11  ALFIFPVLILIKSRLRPKNKKSTAPMVPGAWPLLGHLHLFDTVNPTHVTFGAMADVYGPV 70

Query: 72  FTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMGLSPYGPHWRH 131
           F  KLG  +V+I++S EVA+E +T +D++   RP++ ASKLLGY+ + +  SPYG +WR 
Sbjct: 71  FMAKLGSIKVMIINSKEVAKEIYTVHDKLL-ERPELTASKLLGYNDSFLTFSPYGLYWRE 130

Query: 132 MRKIATLQLLT 143
           +RKIA  +L +
Sbjct: 131 IRKIAVSELFS 140

[Close]

BLAST of MC10g_new0112 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 120.6 bits (301), Expect = 1.2e-27
Identity = 60/146 (41.10%), Postives = 87/146 (59.59%), Query Frame = 0


Query: 12  ALLLFLYALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHITFAKMADAYGPI 71
           AL++F Y   R             P+P GA PL GHLHLL   +      A M+  +GPI
Sbjct: 16  ALVIFGYIFLRKQLSRCEVDSSTIPEPLGALPLFGHLHLLRGKKLLCKKLAAMSQKHGPI 75

Query: 72  FTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMGLSPYGPHWRH 131
           F+LKLG  R+++ S  +  ++CFTTND   ++RP +   + +GY++A + L+PYG +WR 
Sbjct: 76  FSLKLGFYRLVVASDPKTVKDCFTTNDLATATRPNIAFGRYVGYNNASLTLAPYGDYWRE 135

Query: 132 MRKIATLQLLTNHRLEQLQHIIVSEV 158
           +RKI T+ L +NH +E L HI  SEV
Sbjct: 136 LRKIVTVHLFSNHSIEMLGHIRSSEV 161

[Close]

BLAST of MC10g_new0112 vs. TAIR 10
Match: AT3G25180.2 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 120.6 bits (301), Expect = 1.2e-27
Identity = 60/146 (41.10%), Postives = 87/146 (59.59%), Query Frame = 0


Query: 12  ALLLFLYALFRASPISGAHRKRLPPQPAGAWPLIGHLHLLSSTEPAHITFAKMADAYGPI 71
           AL++F Y   R             P+P GA PL GHLHLL   +      A M+  +GPI
Sbjct: 16  ALVIFGYIFLRKQLSRCEVDSSTIPEPLGALPLFGHLHLLRGKKLLCKKLAAMSQKHGPI 75

Query: 72  FTLKLGMNRVLIVSSWEVARECFTTNDRVFSSRPKMVASKLLGYDHAMMGLSPYGPHWRH 131
           F+LKLG  R+++ S  +  ++CFTTND   ++RP +   + +GY++A + L+PYG +WR 
Sbjct: 76  FSLKLGFYRLVVASDPKTVKDCFTTNDLATATRPNIAFGRYVGYNNASLTLAPYGDYWRE 135

Query: 132 MRKIATLQLLTNHRLEQLQHIIVSEV 158
           +RKI T+ L +NH +E L HI  SEV
Sbjct: 136 LRKIVTVHLFSNHSIEMLGHIRSSEV 161

The following BLAST results are available for this feature:

BLAST of MC10g_new0112 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q43068	1.6e-37	52.05	Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2	[more]
A0A0N7F297	1.3e-36	47.33	Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP...	[more]
O49858	5.1e-36	46.71	Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1	[more]
O49394	7.3e-35	51.70	Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=...	[more]
O49859	1.6e-34	46.21	Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1	[more]

BLAST of MC10g_new0112 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022982223.1	2.54e-79	77.56	cytochrome P450 CYP82D47-like [Cucurbita maxima]	[more]
XP_022940489.1	1.86e-78	76.28	cytochrome P450 CYP82D47-like [Cucurbita moschata]	[more]
XP_023523452.1	3.71e-78	76.28	cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo]	[more]
XP_038896981.1	1.11e-75	71.97	cytochrome P450 CYP82D47-like [Benincasa hispida]	[more]
XP_008464766.1	6.28e-74	72.44	PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo] >KAA0032728.1 cytochrome...	[more]

BLAST of MC10g_new0112 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J4B0	1.23e-79	77.56	cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111481116 PE=3 S...	[more]
A0A6J1FKD2	9.01e-79	76.28	cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111446070 PE=3...	[more]
A0A5D3BG48	3.04e-74	72.44	Cytochrome P450 CYP82D47-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3CMB9	3.04e-74	72.44	cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502574 PE=3 SV=1	[more]
A0A5A7SU10	1.55e-71	70.89	Cytochrome P450 CYP82D47-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]

BLAST of MC10g_new0112 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G31970.1	5.2e-36	51.70	cytochrome P450, family 82, subfamily C, polypeptide 2	[more]
AT4G31940.1	3.4e-35	53.66	cytochrome P450, family 82, subfamily C, polypeptide 4	[more]
AT2G25160.1	5.6e-30	45.04	cytochrome P450, family 82, subfamily F, polypeptide 1	[more]
AT3G25180.1	1.2e-27	41.10	cytochrome P450, family 82, subfamily G, polypeptide 1	[more]
AT3G25180.2	1.2e-27	41.10	cytochrome P450, family 82, subfamily G, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 89..110 score: 23.87 coord: 65..84 score: 33.95
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 36..153 e-value: 8.3E-14 score: 51.2
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 28..157 e-value: 7.9E-30 score: 105.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 30..157
None	No IPR available	PANTHER	PTHR47947:SF26	CYTOCHROME P450	coord: 32..157
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 32..157

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g_new0112.1	MC10g_new0112.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098542	defense response to other organism
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen