MC10g_new0079 (gene) Bitter gourd (Dali-11) v1

BLAST of MC10g_new0079 vs. ExPASy Swiss-Prot
Match: Q43667 (Trypsin inhibitor 1 OS=Trichosanthes kirilowii OX=3677 PE=3 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 7.9e-06
Identity = 33/68 (48.53%), Postives = 40/68 (58.82%), Query Frame = 0

Query: 17 VAAVAES--AGADAGEEIQLVSDGVNN------NPIGPCPKILLPCSTDAECKKVCIYWN 76
          +AA  ES  AGA A E IQLVSDGVN         +  CP+IL+PC  + +C + C    
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSEKMMEGVVACPRILMPCKVNDDCLRGC---K 60

BLAST of MC10g_new0079 vs. ExPASy Swiss-Prot
Match: P34950 (Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 2.3e-05
Identity = 32/68 (47.06%), Postives = 41/68 (60.29%), Query Frame = 0

Query: 17 VAAVAESAGADAGEEIQLVSDGVNNNPI--------GPCPKILLPCSTDAECKKVCIYWN 76
          +A+VAES+G    E I+L+SDG N+ P         G CP+IL+PC TD +C   C    
Sbjct: 1  MASVAESSG--VVEVIELISDGGNDLPRKIMSGRHGGICPRILMPCKTDDDCMLDC---R 60

BLAST of MC10g_new0079 vs. NCBI nr
Match: XP_022984819.1 (trypsin inhibitor 1-like [Cucurbita maxima])

HSP 1 Score: 73.6 bits (179), Expect = 4.55e-15
Identity = 40/81 (49.38%), Postives = 53/81 (65.43%), Query Frame = 0

Query: 1  MEWKKFGLVAIVGMLLVAAVAESAGADAGEEIQLVSDGVNNNP------IGPCPKILLPC 60
          MEWK+  L+A+VG+LL+AA AESA    GE IQLVSDGVN+ P      +  CP+IL+ C
Sbjct: 1  MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVNDFPRKMMNGVQGCPRILMEC 60

Query: 61 STDAECKKVCIYWNCLSGYCG 75
            D++C   CI     +G+CG
Sbjct: 61 ELDSDCLPGCICRP--NGFCG 79

BLAST of MC10g_new0079 vs. NCBI nr
Match: KAG6576915.1 (hypothetical protein SDJN03_24489, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 72.4 bits (176), Expect = 9.57e-14
Identity = 41/82 (50.00%), Postives = 54/82 (65.85%), Query Frame = 0

Query: 1   MEWKKFGLVAIVGMLLVAAVAESAGADAGEEIQLVSDGVNNNP-------IGPCPKILLP 60
           MEWK+  L+A+VG+LL+AA AESA    GE IQLVSDGVN+ P       +G CP+IL+ 
Sbjct: 83  MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVNDFPRKMMNGALG-CPRILME 142

Query: 61  CSTDAECKKVCIYWNCLSGYCG 75
           C  D++C   CI     +G+CG
Sbjct: 143 CEVDSDCLPGCICRP--NGFCG 161

BLAST of MC10g_new0079 vs. NCBI nr
Match: KAG7014942.1 (hypothetical protein SDJN02_22573, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 72.4 bits (176), Expect = 9.57e-14
Identity = 41/82 (50.00%), Postives = 54/82 (65.85%), Query Frame = 0

Query: 1   MEWKKFGLVAIVGMLLVAAVAESAGADAGEEIQLVSDGVNNNP-------IGPCPKILLP 60
           MEWK+  L+A+VG+LL+AA AESA    GE IQLVSDGVN+ P       +G CP+IL+ 
Sbjct: 83  MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVNDFPRKMMNGALG-CPRILME 142

Query: 61  CSTDAECKKVCIYWNCLSGYCG 75
           C  D++C   CI     +G+CG
Sbjct: 143 CEVDSDCLPGCICRP--NGFCG 161

BLAST of MC10g_new0079 vs. NCBI nr
Match: KAA0044335.1 (Trypsin inhibitor 1 [Cucumis melo var. makuwa] >TYK29464.1 Trypsin inhibitor 1 [Cucumis melo var. makuwa])

HSP 1 Score: 70.1 bits (170), Expect = 9.60e-14
Identity = 41/77 (53.25%), Postives = 48/77 (62.34%), Query Frame = 0

Query: 1  MEWKKFGLVAIVGMLLVAAVAESAGADAGEEI-QLVSDGVNNNPIGP-CPKILLPCSTDA 60
          MEWKK  LVA++GMLL+A   ES G    EEI QLVSDGVN     P CP+IL+ C TD 
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGFGVDEEIIQLVSDGVNEYSGHPGCPRILMKCKTDR 60

Query: 61 ECKKVCIYWNCLSGYCG 75
          +C   C      +GYCG
Sbjct: 61 DCLAGCTCKR--NGYCG 75

BLAST of MC10g_new0079 vs. NCBI nr
Match: XP_023552845.1 (uncharacterized protein LOC111810370 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 70.9 bits (172), Expect = 3.96e-13
Identity = 39/82 (47.56%), Postives = 54/82 (65.85%), Query Frame = 0

Query: 1   MEWKKFGLVAIVGMLLVAAVAESAGADAGEEIQLVSDGVNNNP-------IGPCPKILLP 60
           MEWK+  L+A+VG+LL+AA A+SA    GE IQLVSDGVN+ P       +G CP+IL+ 
Sbjct: 85  MEWKRGALIAMVGLLLMAAFADSAAVHGGEVIQLVSDGVNDFPRKMMNAALG-CPRILME 144

Query: 61  CSTDAECKKVCIYWNCLSGYCG 75
           C  D++C   C+     +G+CG
Sbjct: 145 CEVDSDCLPGCVCRP--NGFCG 163

BLAST of MC10g_new0079 vs. ExPASy TrEMBL
Match: A0A6J1JBL8 (trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482991 PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.20e-15
Identity = 40/81 (49.38%), Postives = 53/81 (65.43%), Query Frame = 0

Query: 1  MEWKKFGLVAIVGMLLVAAVAESAGADAGEEIQLVSDGVNNNP------IGPCPKILLPC 60
          MEWK+  L+A+VG+LL+AA AESA    GE IQLVSDGVN+ P      +  CP+IL+ C
Sbjct: 1  MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVNDFPRKMMNGVQGCPRILMEC 60

Query: 61 STDAECKKVCIYWNCLSGYCG 75
            D++C   CI     +G+CG
Sbjct: 61 ELDSDCLPGCICRP--NGFCG 79

BLAST of MC10g_new0079 vs. ExPASy TrEMBL
Match: A0A5D3E0W0 (Trypsin inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00600 PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 4.65e-14
Identity = 41/77 (53.25%), Postives = 48/77 (62.34%), Query Frame = 0

Query: 1  MEWKKFGLVAIVGMLLVAAVAESAGADAGEEI-QLVSDGVNNNPIGP-CPKILLPCSTDA 60
          MEWKK  LVA++GMLL+A   ES G    EEI QLVSDGVN     P CP+IL+ C TD 
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGFGVDEEIIQLVSDGVNEYSGHPGCPRILMKCKTDR 60

Query: 61 ECKKVCIYWNCLSGYCG 75
          +C   C      +GYCG
Sbjct: 61 DCLAGCTCKR--NGYCG 75

BLAST of MC10g_new0079 vs. ExPASy TrEMBL
Match: A0A0A0KY34 (Trypsin inhibitor 1 OS=Cucumis sativus OX=3659 GN=Csa_4G000810 PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 6.28e-13
Identity = 43/84 (51.19%), Postives = 51/84 (60.71%), Query Frame = 0

Query: 1  MEWKKFGLVAIVGMLLVAAVAESAGADAGEEI-QLVSDGVN--NNPIGP-----CPKILL 60
          MEWKK  LVA++GMLL+A   ES G    EEI QLVSDGVN  +  IG      CP+IL+
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60

Query: 61 PCSTDAECKKVCIYWNCLS-GYCG 75
           C TD++C   C    C   GYCG
Sbjct: 61 KCKTDSDCYPGC---TCKPMGYCG 81

BLAST of MC10g_new0079 vs. ExPASy TrEMBL
Match: A0A0A0KT01 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000805 PE=3 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 8.62e-08
Identity = 36/85 (42.35%), Postives = 49/85 (57.65%), Query Frame = 0

Query: 1  MEWKKFGLVAIVGMLLVAAVAESAGADAGEEIQLVSDGVNNNPIGP---------CPKIL 60
          MEWKK G++A+VGML++AA+ E     A  E + V  G+N N +           CP+IL
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDC--HAAIESESVK-GLNKNELPRKMMNEETRMCPRIL 60

Query: 61 LPCSTDAECKKVCIYWNCLS-GYCG 75
            C+TD +C   CI   CLS G+CG
Sbjct: 61 EECTTDDDCMNDCI---CLSNGFCG 79

BLAST of MC10g_new0079 vs. ExPASy TrEMBL
Match: A0A4P2SJP1 (Trypsin inhibitor I1 OS=Trichosanthes kirilowii OX=3677 PE=2 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 2.03e-06
Identity = 33/67 (49.25%), Postives = 39/67 (58.21%), Query Frame = 0

Query: 18 AAVAESA--GADAGEEIQLVSDGVNN------NPIGPCPKILLPCSTDAECKKVCIYWNC 75
          AA  ESA  GA A E IQLVSDGVN         +  CP+IL+PC  + +C + C    C
Sbjct: 2  AAFVESARAGAGADEVIQLVSDGVNEYSEKMMKGVVGCPRILMPCKVNDDCLRGC---KC 61