MC10g_new0038 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g_new0038
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Pectinesterase
Location	MC10: 1522405 .. 1524655 (+)
RNA-Seq Expression	MC10g_new0038
Synteny	MC10g_new0038

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTCAAACCCAGCCAACCTGTCTCTCCATTTCATCCTAATTTGGCTCTCCTTCCCTATATAACCAAACCTGAAGTAGCTTGCTTAGCCTCATCAAAGCAAGAACCTGCTCTTCTAATGGAGAAGAGTTTTGGGGTGTTGTTTTTTCTTCTCTTCTTTTCACTCTCTTCTCCAGCCTTCTCCAAGCATGCCTCTAATGGCATAGATTGGTGGTGCAGCAAAACCACGTACCCTGAGACCTGCAAGTATTTCTTCAACCATGGCAGCAATGCTGCTCCCAAAGATATGTCTGAGTTCAAGAAAATGGTGACCAAATTCGCCATGGAGAGAGCTTTGAGAGCCGAGAGCCATGCTAAGGGTGTTGGTTCCAAGTGCCGAAATGAGAAGGAAAAGGCTGCTTGGGCTGATTGCTTGGAGCTCTACCAAAACACCATCCTTCAGCTCAACCACACTCTAGATTCCTCCTCTAAATGTACTGAGTTTGATATCCAAACCTGGCTCAGTACCGCCCTAACCAACCTTGAAACGTGCTACACCGGGTTTGCTGAGTTTAATGTTTCGGACCATGTATTGCCGCTGATCATGTCGAACAATGTCACAAAGCTGATAAGCAACAGCTTGGCGATTAACAATGCTTCAGCTTGGCCGGGGAAAGAGACTTATAAAGAGGGTTTTCCAAGTTGGCTTTTAGGTGGCGACAGGAGGCTACTGCAGTCATCTTCAGTAAAGACAGACCTTGTCGTGGCTCAGGACGGATCCGGTGACCATAGGACAGTTGGAGCGGCTCTAGAGGCAGCTGCAAAAAGGAAGACAGGTGGAAGATTTGTTATACATGTAAAGAGAGGGGTTTACGAAGAAAACCTCGAGATAGGAAATAAGATGAAGAATATAATGCTCGTCGGAGATGGAATGAGATTTACCATCATTACTGGTAGTCGAAGTGTTAGAGGAGGCTCCACCACTTTCCATTCTGCTACTGTTGGTAAGTCCCTCTCCCTCTCTTTGTGTTGACTGTTTATAAGGTCATTTACCTCCATTTCAACAACATTTGAATCGGTAAACCTGTAGATTTTACTAATTCCAAGTCCATCAACCCTATTAGCAGATTTGTCATCAAGAAAGTAAACACTAGACAAAATTTCTTGCGCCCCAAAACATGATACTTTGAATTTTCTCCCACAGCCCATTTCCAAACAAAAATATTAGTTTTCAAAGGTAGAGTCATCTGTGATCAACATCTTTGTCAAGATCTCAATATAAAAAATGAAAAATTGTCCATGGCATTTCATCATAAATGCATCATCGAAGTATACAAGAAATGACCATCAAATGTCAATTAATTATATGCATTTCACATGTTCTAAAAAAATTTATATCAAGAAAATGTGAGATCAAATTCACCTCTTTCATTTTGTTTTTTCATAAAACTCCTCCAAATTCACCTCTTTCATTTGGTTTTTCCATAAAACTCCTCAAATATATGAATTTTTTCATAAGACCAATCATGTGAAAAGTATCCGATTAAAATGAGATACTTACTTGTCTTCTATGATAATTAGCGGTGACTGGTGAAGGATTTATAGCTCGTGGTATCACATTCCGAAACACTGCTGGTCCTGAAAACCATCAAGCAGTTGCTCTCCGATCTGGCGCTGATTTTTCTGTCTTCTACCGCTGTGGCTTTGAAGGGTATCAGGATACCCTCTATGTCCATTCACAGAGACAATTCTATAAAGAGTGTTATATTTATGGCACGGTAGATTTTATATTCGGCAATGCTGCAGTTGTTCTACAGAACTGCATGATTTATGCAAGGAGGCCAATGAATGGTCAGAAGAATGCAGTCACTGCTCAAGGACGGACTGATCCAAACCAAAATACTGGCATTTCGATCCACAACTCGCGTGTCATGGCCACAGATGACCTAAAACAAGTGGAGAGCACAGTGAAAACCTACTTGGGAAGGCCGTGGAAGGAGTATTCTCGAACAGTTTTCATGCAAACTTACCTCGATAGTTTGGTTGATCCAGCAGGTTGGTTGGAGTGGGATGGTGAATTTGCACTGAATACTTTATATTATGGAGAGTATAAGAACATGGGTCCTGGTTCATCAACAAGCCAGAGAGTGAAGTGGAGGGGTTACCACATTGTAACCAATTCAACAGAGGCATCAAAGTTCACCGTTCAAAATTTCATTGCAGGGCAGTCATGGCTGCCAGGCACAGAAGTCCCATTTACTCCTGGACTCTAA

mRNA sequence

ATGTTCAAACCCAGCCAACCTGTCTCTCCATTTCATCCTAATTTGGCTCTCCTTCCCTATATAACCAAACCTGAAGTAGCTTGCTTAGCCTCATCAAAGCAAGAACCTGCTCTTCTAATGGAGAAGAGTTTTGGGGTGTTGTTTTTTCTTCTCTTCTTTTCACTCTCTTCTCCAGCCTTCTCCAAGCATGCCTCTAATGGCATAGATTGGTGGTGCAGCAAAACCACGTACCCTGAGACCTGCAAGTATTTCTTCAACCATGGCAGCAATGCTGCTCCCAAAGATATGTCTGAGTTCAAGAAAATGGTGACCAAATTCGCCATGGAGAGAGCTTTGAGAGCCGAGAGCCATGCTAAGGGTGTTGGTTCCAAGTGCCGAAATGAGAAGGAAAAGGCTGCTTGGGCTGATTGCTTGGAGCTCTACCAAAACACCATCCTTCAGCTCAACCACACTCTAGATTCCTCCTCTAAATGTACTGAGTTTGATATCCAAACCTGGCTCAGTACCGCCCTAACCAACCTTGAAACGTGCTACACCGGGTTTGCTGAGTTTAATGTTTCGGACCATGTATTGCCGCTGATCATGTCGAACAATGTCACAAAGCTGATAAGCAACAGCTTGGCGATTAACAATGCTTCAGCTTGGCCGGGGAAAGAGACTTATAAAGAGGGTTTTCCAAGTTGGCTTTTAGGTGGCGACAGGAGGCTACTGCAGTCATCTTCAGTAAAGACAGACCTTGTCGTGGCTCAGGACGGATCCGGTGACCATAGGACAGTTGGAGCGGCTCTAGAGGCAGCTGCAAAAAGGAAGACAGGTGGAAGATTTGTTATACATGTAAAGAGAGGGGTTTACGAAGAAAACCTCGAGATAGGAAATAAGATGAAGAATATAATGCTCGTCGGAGATGGAATGAGATTTACCATCATTACTGGTAGTCGAAGTGTTAGAGGAGGCTCCACCACTTTCCATTCTGCTACTGTTGCGGTGACTGGTGAAGGATTTATAGCTCGTGGTATCACATTCCGAAACACTGCTGGTCCTGAAAACCATCAAGCAGTTGCTCTCCGATCTGGCGCTGATTTTTCTGTCTTCTACCGCTGTGGCTTTGAAGGGTATCAGGATACCCTCTATGTCCATTCACAGAGACAATTCTATAAAGAGTGTTATATTTATGGCACGGTAGATTTTATATTCGGCAATGCTGCAGTTGTTCTACAGAACTGCATGATTTATGCAAGGAGGCCAATGAATGGTCAGAAGAATGCAGTCACTGCTCAAGGACGGACTGATCCAAACCAAAATACTGGCATTTCGATCCACAACTCGCGTGTCATGGCCACAGATGACCTAAAACAAGTGGAGAGCACAGTGAAAACCTACTTGGGAAGGCCGTGGAAGGAGTATTCTCGAACAGTTTTCATGCAAACTTACCTCGATAGTTTGGTTGATCCAGCAGGTTGGTTGGAGTGGGATGGTGAATTTGCACTGAATACTTTATATTATGGAGAGTATAAGAACATGGGTCCTGGTTCATCAACAAGCCAGAGAGTGAAGTGGAGGGGTTACCACATTGTAACCAATTCAACAGAGGCATCAAAGTTCACCGTTCAAAATTTCATTGCAGGGCAGTCATGGCTGCCAGGCACAGAAGTCCCATTTACTCCTGGACTCTAA

Coding sequence (CDS)

ATGTTCAAACCCAGCCAACCTGTCTCTCCATTTCATCCTAATTTGGCTCTCCTTCCCTATATAACCAAACCTGAAGTAGCTTGCTTAGCCTCATCAAAGCAAGAACCTGCTCTTCTAATGGAGAAGAGTTTTGGGGTGTTGTTTTTTCTTCTCTTCTTTTCACTCTCTTCTCCAGCCTTCTCCAAGCATGCCTCTAATGGCATAGATTGGTGGTGCAGCAAAACCACGTACCCTGAGACCTGCAAGTATTTCTTCAACCATGGCAGCAATGCTGCTCCCAAAGATATGTCTGAGTTCAAGAAAATGGTGACCAAATTCGCCATGGAGAGAGCTTTGAGAGCCGAGAGCCATGCTAAGGGTGTTGGTTCCAAGTGCCGAAATGAGAAGGAAAAGGCTGCTTGGGCTGATTGCTTGGAGCTCTACCAAAACACCATCCTTCAGCTCAACCACACTCTAGATTCCTCCTCTAAATGTACTGAGTTTGATATCCAAACCTGGCTCAGTACCGCCCTAACCAACCTTGAAACGTGCTACACCGGGTTTGCTGAGTTTAATGTTTCGGACCATGTATTGCCGCTGATCATGTCGAACAATGTCACAAAGCTGATAAGCAACAGCTTGGCGATTAACAATGCTTCAGCTTGGCCGGGGAAAGAGACTTATAAAGAGGGTTTTCCAAGTTGGCTTTTAGGTGGCGACAGGAGGCTACTGCAGTCATCTTCAGTAAAGACAGACCTTGTCGTGGCTCAGGACGGATCCGGTGACCATAGGACAGTTGGAGCGGCTCTAGAGGCAGCTGCAAAAAGGAAGACAGGTGGAAGATTTGTTATACATGTAAAGAGAGGGGTTTACGAAGAAAACCTCGAGATAGGAAATAAGATGAAGAATATAATGCTCGTCGGAGATGGAATGAGATTTACCATCATTACTGGTAGTCGAAGTGTTAGAGGAGGCTCCACCACTTTCCATTCTGCTACTGTTGCGGTGACTGGTGAAGGATTTATAGCTCGTGGTATCACATTCCGAAACACTGCTGGTCCTGAAAACCATCAAGCAGTTGCTCTCCGATCTGGCGCTGATTTTTCTGTCTTCTACCGCTGTGGCTTTGAAGGGTATCAGGATACCCTCTATGTCCATTCACAGAGACAATTCTATAAAGAGTGTTATATTTATGGCACGGTAGATTTTATATTCGGCAATGCTGCAGTTGTTCTACAGAACTGCATGATTTATGCAAGGAGGCCAATGAATGGTCAGAAGAATGCAGTCACTGCTCAAGGACGGACTGATCCAAACCAAAATACTGGCATTTCGATCCACAACTCGCGTGTCATGGCCACAGATGACCTAAAACAAGTGGAGAGCACAGTGAAAACCTACTTGGGAAGGCCGTGGAAGGAGTATTCTCGAACAGTTTTCATGCAAACTTACCTCGATAGTTTGGTTGATCCAGCAGGTTGGTTGGAGTGGGATGGTGAATTTGCACTGAATACTTTATATTATGGAGAGTATAAGAACATGGGTCCTGGTTCATCAACAAGCCAGAGAGTGAAGTGGAGGGGTTACCACATTGTAACCAATTCAACAGAGGCATCAAAGTTCACCGTTCAAAATTTCATTGCAGGGCAGTCATGGCTGCCAGGCACAGAAGTCCCATTTACTCCTGGACTCTAA

Protein sequence

MFKPSQPVSPFHPNLALLPYITKPEVACLASSKQEPALLMEKSFGVLFFLLFFSLSSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSNAAPKDMSEFKKMVTKFAMERALRAESHAKGVGSKCRNEKEKAAWADCLELYQNTILQLNHTLDSSSKCTEFDIQTWLSTALTNLETCYTGFAEFNVSDHVLPLIMSNNVTKLISNSLAINNASAWPGKETYKEGFPSWLLGGDRRLLQSSSVKTDLVVAQDGSGDHRTVGAALEAAAKRKTGGRFVIHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGADFSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVESTVKTYLGRPWKEYSRTVFMQTYLDSLVDPAGWLEWDGEFALNTLYYGEYKNMGPGSSTSQRVKWRGYHIVTNSTEASKFTVQNFIAGQSWLPGTEVPFTPGL

Homology

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BLAST of MC10g_new0038 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 629.0 bits (1621), Expect = 5.0e-179
Identity = 315/509 (61.89%), Postives = 395/509 (77.60%), Query Frame = 0


Query: 50  LLFFSLSSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSNAAP-KDMSEFKKMVTKFAM 109
           L+ FSLS  +F  ++   +  WC KT  P+ C+YF    ++    K  ++F K+  + A+
Sbjct: 11  LVLFSLSHTSFG-YSPEEVKSWCGKTPNPQPCEYFLTQKTDVTSIKQDTDFYKISLQLAL 70

Query: 110 ERALRAESHAKGVGSKCRNEKEKAAWADCLELYQNTILQLNHTLDSSSKCTEFDIQTWLS 169
           ERA  A+S    +GSKCRNE+EKAAW DC ELY+ T+L+LN T +SS  CT+ D QTWLS
Sbjct: 71  ERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKVDKQTWLS 130

Query: 170 TALTNLETCYTGFAEFNVSDHVLPLIMSNNVTKLISNSLAINNASAWPGKETYKEGFPSW 229
           TALTNLETC     +  V ++VLPL +SNNVTKLISN+L++N       + +YK+GFP+W
Sbjct: 131 TALTNLETCRASLEDLGVPEYVLPL-LSNNVTKLISNTLSLNKVPY--NEPSYKDGFPTW 190

Query: 230 LLGGDRRLLQSSSVKTDLVVAQDGSGDHRTVGAALEAAAKRKTGGRFVIHVKRGVYEENL 289
           +  GDR+LLQ++  + ++VVAQDGSG+ +T+  A+ AAA R  G R+VI++K G Y EN+
Sbjct: 191 VKPGDRKLLQTTP-RANIVVAQDGSGNVKTIQEAV-AAASRAGGSRYVIYIKAGTYNENI 250

Query: 290 EIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIARGITFRNTAGPE 349
           E+  K+KNIM VGDG+  TIITGS+SV GG+TTF SATVAV G+ FIAR IT RNTAGP 
Sbjct: 251 EV--KLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPN 310

Query: 350 NHQAVALRSGADFSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNC 409
           NHQAVALRSG+D SVFYRC FEGYQDTLYVHSQRQFY+EC IYGTVDFIFGNAAVVLQNC
Sbjct: 311 NHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNC 370

Query: 410 MIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVESTVKTYLGRPWKEY 469
            I+AR+P N + N +TAQGRTDPNQ+TGI IHN RV A  DLK V+S+VKT+LGRPWK+Y
Sbjct: 371 NIFARKPPN-RTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQY 430

Query: 470 SRTVFMQTYLDSLVDPAGWLEWDGEFALNTLYYGEYKNMGPGSSTSQRVKWRGYHIVTNS 529
           SRTV+++T+LDSL++PAGW+EW G+FALNTLYY EY N GPGSST+ RVKWRGYH++T+ 
Sbjct: 431 SRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSP 490

Query: 530 TEASKFTVQNFIAGQSWLPGTEVPFTPGL 558
           ++ S+FTV NFIAG SWLP T VPFT GL
Sbjct: 491 SQVSQFTVGNFIAGNSWLPATNVPFTSGL 510

[Close]

BLAST of MC10g_new0038 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 602.4 bits (1552), Expect = 5.0e-171
Identity = 303/513 (59.06%), Postives = 386/513 (75.24%), Query Frame = 0


Query: 46  VLFFLLFFSLSSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSNAAP-KDMSEFKKMVT 105
           ++ F++   L +   S +    +  WCS+T  P+ C+YF  H SN  P K  SEF K+  
Sbjct: 8   IINFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISM 67

Query: 106 KFAMERALRAESHAKGVGSKCRNEKEKAAWADCLELYQNTILQLNHTLDSSSKCTEFDIQ 165
           K  ++RA+ A++HA  +G KCR+ +EKAAW DC++LY  T+ ++N T+D + KC++ D Q
Sbjct: 68  KLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQ 127

Query: 166 TWLSTALTNLETCYTGFAEFNVSDHVLPLIMSNNVTKLISNSLAINNASAWPGKETYKEG 225
           TWLSTALTNL+TC  GF E  V+D VLPL MSNNV+ L+ N+LAIN    +      K+G
Sbjct: 128 TWLSTALTNLDTCRAGFLELGVTDIVLPL-MSNNVSNLLCNTLAINKV-PFNYTPPEKDG 187

Query: 226 FPSWLLGGDRRLLQSSSVKTDLVVAQDGSGDHRTVGAALEAAAKRKTGGRFVIHVKRGVY 285
           FPSW+  GDR+LLQSS+ K + VVA+DGSG+ +T+  A++AA+     GRFVI+VK+GVY
Sbjct: 188 FPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAAS---GSGRFVIYVKQGVY 247

Query: 286 EENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIARGITFRNT 345
            ENLEI  + KN+ML GDG+  TIITGS+SV GG+TTF+SATVA  G+GFIARGITFRNT
Sbjct: 248 SENLEI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNT 307

Query: 346 AGPENHQAVALRSGADFSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVV 405
           AG  N QAVALRSG+D SVFY+C FE YQDTLYVHS RQFY++C +YGTVDFIFGNAA V
Sbjct: 308 AGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAV 367

Query: 406 LQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVESTVKTYLGRP 465
           LQNC I+ARRP   + N +TAQGR+DPNQNTGI IHNSRV A  DL+ V  + KTYLGRP
Sbjct: 368 LQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRP 427

Query: 466 WKEYSRTVFMQTYLDSLVDPAGWLEWDGEFALNTLYYGEYKNMGPGSSTSQRVKWRGYHI 525
           W++YSRTVFM+T LDSL+DP GWLEWDG FAL TL+Y E++N GPG+STS RV W G+ +
Sbjct: 428 WRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRV 487

Query: 526 VTNSTEASKFTVQNFIAGQSWLPGTEVPFTPGL 558
           + +++EASKFTV  F+AG SW+P + VPFT GL
Sbjct: 488 LGSASEASKFTVGTFLAGGSWIP-SSVPFTSGL 511

[Close]

BLAST of MC10g_new0038 vs. ExPASy Swiss-Prot
Match: Q9FHN5 (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 8.1e-161
Identity = 289/537 (53.82%), Postives = 371/537 (69.09%), Query Frame = 0


Query: 39  LMEKSFGVLF---FLLFFSLSSPAFSKHASNGIDWWCSKTTYPETCK-YFFNHGSNAAPK 98
           +M +   +LF    LL      P  +    N  D WC KT YP+ CK YF NH     P 
Sbjct: 3   MMMQKLSILFLHLILLVLLCVHPLTTVADRNSTD-WCDKTPYPDPCKCYFKNHNGFQQPT 62

Query: 99  DMSEFKKMVTKFAMERALRAESHAKGVGSKCRNEKEKAAWADCLELYQNTILQLNHTL-- 158
            +SEF+ M+ + AM+RA+ A +     G  C + K++A  ADC++LY +TI+QLN TL  
Sbjct: 63  QLSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHG 122

Query: 159 -----DSSSKCTEFDIQTWLSTALTNLETCYTGFAEFNVSDHVLPLIMSNNVTKLISNSL 218
                 ++  CT+FD QTWLSTALTN ETC  G ++ NV+D + P++ +  ++ LISN L
Sbjct: 123 VSPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCL 182

Query: 219 AINNASAWPGKE----TYKEGFPSWLLGGDRRLLQSSSVKTDLVVAQDGSGDHRTVGAAL 278
           A+N A    G +      ++GFP+WL   D+RLL+  +V+ +LVVA+DGSG   TV AA+
Sbjct: 183 AVNGALLTAGNKGNTTANQKGFPTWLSRKDKRLLR--AVRANLVVAKDGSGHFNTVQAAI 242

Query: 279 EAAAKRK-TGGRFVIHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTF 338
           + A +RK T GRFVI+VKRG+Y+EN+ +     +IMLVGDGMR TIITG RSV+GG TT+
Sbjct: 243 DVAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTY 302

Query: 339 HSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGADFSVFYRCGFEGYQDTLYVHSQR 398
           +SAT  + G  FIA+GITFRNTAGP   QAVALRS +D S+FY+C  EGYQDTL VHSQR
Sbjct: 303 NSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQR 362

Query: 399 QFYKECYIYGTVDFIFGNAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNS 458
           QFY+ECYIYGTVDFIFGNAA V QNC+I  RRP+ GQ N +TAQGR DP QNTGISIHNS
Sbjct: 363 QFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNS 422

Query: 459 RVMATDDLKQVESTVKTYLGRPWKEYSRTVFMQTYLDSLVDPAGWLEW--DGEFALNTLY 518
           R++   DLK V  TVKTY+GRPW ++SRTV +QTYLD++V P GW  W     F L+TL+
Sbjct: 423 RILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLF 482

Query: 519 YGEYKNMGPGSSTSQRVKWRGYHIVTNSTEASKFTVQNFIAGQSWLPGTEVPFTPGL 558
           Y EYKN GP SST  RV W+G+H++  +++AS FTV  FIAG +WLP T +PFT GL
Sbjct: 483 YAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

[Close]

BLAST of MC10g_new0038 vs. ExPASy Swiss-Prot
Match: Q9FHN4 (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3702 GN=PME60 PE=2 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 5.8e-159
Identity = 280/506 (55.34%), Postives = 356/506 (70.36%), Query Frame = 0


Query: 65  SNGIDWWCSKTTYPETCK-YFFNHGSNAAPKDMSEFKKMVTKFAMERALRAESHAKGVGS 124
           S  ID WC KT YP  CK YF  H     P  +SEF+ ++ + AM+RA+ A         
Sbjct: 35  STNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQISEFRVLLVEAAMDRAVSAWDKLTNSSK 94

Query: 125 KCRNEKEKAAWADCLELYQNTILQLNHTLDSSS-----KCTEFDIQTWLSTALTNLETCY 184
            C + K++A  ADC+ LY +T++QLN TL   S     +CT+FD QTWLSTALTN ETC 
Sbjct: 95  NCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKTGRRCTDFDAQTWLSTALTNTETCR 154

Query: 185 TGFAEFNVSDHVLPLIMSNNVTKLISNSLAINNASAWPGKETY----KEGFPSWLLGGDR 244
            G ++ NVSD   P++ +  ++ LISN LA+N A    GK        +GFP+W+   +R
Sbjct: 155 RGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALLTAGKNDSTTGDSKGFPTWVSRKER 214

Query: 245 RLLQSSSVKTDLVVAQDGSGDHRTVGAALEAAAKRK-TGGRFVIHVKRGVYEENLEIGNK 304
           RLLQ  SV+ +LVVA+DGSG  +TV AA++ A +RK T GRFVI+VKRG+Y+ENL +   
Sbjct: 215 RLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRKVTSGRFVIYVKRGIYQENLNVRLN 274

Query: 305 MKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIARGITFRNTAGPENHQAV 364
             NIMLVGDGMR+TIITG RSV+GG TT+ SAT  + G  FIA+GI F+NTAGP   QAV
Sbjct: 275 NDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGIEGLHFIAKGIAFQNTAGPAKGQAV 334

Query: 365 ALRSGADFSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYAR 424
           ALRS +D S+FYRC  EGYQDTL VHSQRQFY+ECYIYGTVDFIFGNAAVV QNC+I  R
Sbjct: 335 ALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYRECYIYGTVDFIFGNAAVVFQNCIILPR 394

Query: 425 RPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVESTVKTYLGRPWKEYSRTVF 484
            P+ GQ N +TAQGRTD  QNTGISIHNS ++   DLK V  +VKTY+GRPW  YSRTV 
Sbjct: 395 LPLKGQANVITAQGRTDLFQNTGISIHNSIIIPAPDLKPVVRSVKTYMGRPWMMYSRTVV 454

Query: 485 MQTYLDSLVDPAGWLEW--DGEFALNTLYYGEYKNMGPGSSTSQRVKWRGYHIVTNSTEA 544
           ++TY+DS+V P GW  W     + L+TL+Y EYKN+GP SST  RV+W+G+H+++ +++A
Sbjct: 455 LKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYKNIGPASSTRWRVRWKGFHVLSKASDA 514

Query: 545 SKFTVQNFIAGQSWLPGTEVPFTPGL 558
           S F+V  FIAG +WLPG+ +PFT  L
Sbjct: 515 SAFSVGKFIAGTAWLPGSGIPFTSEL 540

[Close]

BLAST of MC10g_new0038 vs. ExPASy Swiss-Prot
Match: Q9STY3 (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3702 GN=PME33 PE=2 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 6.0e-156
Identity = 279/522 (53.45%), Postives = 357/522 (68.39%), Query Frame = 0


Query: 57  SPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSN-----AAPKDMSEFKKMVTKFAMERA 116
           SP  +   S  + WWC+KT + ETC Y+F   S        P+  SEF +M+ K A+++A
Sbjct: 73  SPINNGSVSGDMTWWCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQA 132

Query: 117 LRAESHAKGVGSKCRNEKEKAAWADCLELYQNTILQLNHTL-------DSSSKCTEFDIQ 176
           +   S     G  C N + KAAW+DC+ L+QNT+ QLN TL        S  KCT+FD Q
Sbjct: 133 VITHSQTVKFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQ 192

Query: 177 TWLSTALTNLETCYTGFAEFNVSDHVLPLIMSNNVTKLISNSLAINNASAWPGKET---- 236
           TWLSTA TN+ETC +G  + NVSD V+P+I + N++ LI N LA+N         T    
Sbjct: 193 TWLSTAQTNIETCRSGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTAN 252

Query: 237 YKEGFPSWLLGGDRRLLQSSSV---KTDLVVAQDGSGDHRTVGAALEAAAKRKTGGRFVI 296
           +KE FPSW+   +RRLL S+S+      LVVAQD SG  R++ AA+  AA+R+   RFVI
Sbjct: 253 HKEYFPSWVSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVI 312

Query: 297 HVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIAR 356
           +VK+GVY EN+++GN   NIMLVGDG R TIIT  RSV+ G TT++SAT    G+ F+A+
Sbjct: 313 YVKKGVYRENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAK 372

Query: 357 GITFRNTAGPENHQAVALRSGADFSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFI 416
            +TF NTAGP   QAVA+RS +D SVFYR G  G+QDTLY+HSQRQF++ECYI GT+DFI
Sbjct: 373 DMTFINTAGPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFI 432

Query: 417 FGNAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVESTV 476
           FGNAAVV QNCMI  RRP++GQ N +TAQGR DP QNTGI+IH+SR++A  DLK V    
Sbjct: 433 FGNAAVVFQNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAY 492

Query: 477 KTYLGRPWKEYSRTVFMQTYLDSLVDPAGWLEW--DGEFALNTLYYGEYKNMGPGSSTSQ 536
           KTYLGRPW+ YSR   M+TY+D+ + P GW  W     FALNT++YGEYKN GPGSST  
Sbjct: 493 KTYLGRPWQAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRW 552

Query: 537 RVKWRGYHIVTNSTEASKFTVQNFIAGQSWLPGTEVPFTPGL 558
           RV+W+G+H +T++  AS+FTV + IAG SWLP T VPF  GL
Sbjct: 553 RVRWKGFHAITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

[Close]

BLAST of MC10g_new0038 vs. NCBI nr
Match: XP_022141175.1 (pectinesterase-like [Momordica charantia])

HSP 1 Score: 1132 bits (2927), Expect = 0.0
Identity = 557/557 (100.00%), Postives = 557/557 (100.00%), Query Frame = 0


Query: 1   MFKPSQPVSPFHPNLALLPYITKPEVACLASSKQEPALLMEKSFGVLFFLLFFSLSSPAF 60
           MFKPSQPVSPFHPNLALLPYITKPEVACLASSKQEPALLMEKSFGVLFFLLFFSLSSPAF
Sbjct: 1   MFKPSQPVSPFHPNLALLPYITKPEVACLASSKQEPALLMEKSFGVLFFLLFFSLSSPAF 60

Query: 61  SKHASNGIDWWCSKTTYPETCKYFFNHGSNAAPKDMSEFKKMVTKFAMERALRAESHAKG 120
           SKHASNGIDWWCSKTTYPETCKYFFNHGSNAAPKDMSEFKKMVTKFAMERALRAESHAKG
Sbjct: 61  SKHASNGIDWWCSKTTYPETCKYFFNHGSNAAPKDMSEFKKMVTKFAMERALRAESHAKG 120

Query: 121 VGSKCRNEKEKAAWADCLELYQNTILQLNHTLDSSSKCTEFDIQTWLSTALTNLETCYTG 180
           VGSKCRNEKEKAAWADCLELYQNTILQLNHTLDSSSKCTEFDIQTWLSTALTNLETCYTG
Sbjct: 121 VGSKCRNEKEKAAWADCLELYQNTILQLNHTLDSSSKCTEFDIQTWLSTALTNLETCYTG 180

Query: 181 FAEFNVSDHVLPLIMSNNVTKLISNSLAINNASAWPGKETYKEGFPSWLLGGDRRLLQSS 240
           FAEFNVSDHVLPLIMSNNVTKLISNSLAINNASAWPGKETYKEGFPSWLLGGDRRLLQSS
Sbjct: 181 FAEFNVSDHVLPLIMSNNVTKLISNSLAINNASAWPGKETYKEGFPSWLLGGDRRLLQSS 240

Query: 241 SVKTDLVVAQDGSGDHRTVGAALEAAAKRKTGGRFVIHVKRGVYEENLEIGNKMKNIMLV 300
           SVKTDLVVAQDGSGDHRTVGAALEAAAKRKTGGRFVIHVKRGVYEENLEIGNKMKNIMLV
Sbjct: 241 SVKTDLVVAQDGSGDHRTVGAALEAAAKRKTGGRFVIHVKRGVYEENLEIGNKMKNIMLV 300

Query: 301 GDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGAD 360
           GDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGAD
Sbjct: 301 GDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGAD 360

Query: 361 FSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARRPMNGQK 420
           FSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARRPMNGQK
Sbjct: 361 FSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARRPMNGQK 420

Query: 421 NAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVESTVKTYLGRPWKEYSRTVFMQTYLDS 480
           NAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVESTVKTYLGRPWKEYSRTVFMQTYLDS
Sbjct: 421 NAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVESTVKTYLGRPWKEYSRTVFMQTYLDS 480

Query: 481 LVDPAGWLEWDGEFALNTLYYGEYKNMGPGSSTSQRVKWRGYHIVTNSTEASKFTVQNFI 540
           LVDPAGWLEWDGEFALNTLYYGEYKNMGPGSSTSQRVKWRGYHIVTNSTEASKFTVQNFI
Sbjct: 481 LVDPAGWLEWDGEFALNTLYYGEYKNMGPGSSTSQRVKWRGYHIVTNSTEASKFTVQNFI 540

Query: 541 AGQSWLPGTEVPFTPGL 557
           AGQSWLPGTEVPFTPGL
Sbjct: 541 AGQSWLPGTEVPFTPGL 557

[Close]

BLAST of MC10g_new0038 vs. NCBI nr
Match: XP_038882414.1 (pectinesterase-like [Benincasa hispida])

HSP 1 Score: 928 bits (2399), Expect = 0.0
Identity = 456/518 (88.03%), Postives = 479/518 (92.47%), Query Frame = 0


Query: 40  MEKSFGVLFFLLFFSLSSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSNAAPKDMSEF 99
           ME+S GV FFL FF LSS AFSKHASNGIDWWC KTTYPETCKYFFN GS AAPKDM+EF
Sbjct: 1   MERSLGVFFFLHFFLLSSAAFSKHASNGIDWWCRKTTYPETCKYFFNQGSKAAPKDMTEF 60

Query: 100 KKMVTKFAMERALRAESHAKGVGSKCRNEKEKAAWADCLELYQNTILQLNHTLDSSSKCT 159
           KKMV +FAMERAL AESH KGVGSKCRN KEKAAWADCL+LYQNTI QLN TLDS++KCT
Sbjct: 61  KKMVIQFAMERALGAESHTKGVGSKCRNGKEKAAWADCLKLYQNTIFQLNQTLDSATKCT 120

Query: 160 EFDIQTWLSTALTNLETCYTGFAEFNVSDHVLPLIMSNNVTKLISNSLAINNASAWPGKE 219
           EFDIQTWLSTALTNLETC TGFAE NVSD++L LIMSNNVTKLISNSLAINNASA  GKE
Sbjct: 121 EFDIQTWLSTALTNLETCRTGFAELNVSDYILQLIMSNNVTKLISNSLAINNASAGLGKE 180

Query: 220 TYKEGFPSWLLGGDRRLLQSSSVKTDLVVAQDGSGDHRTVGAALEAAAKRKTGGRFVIHV 279
           TYK+GFPSWL GGDRRLLQSSS K DLVVAQDGSG+H TVGAALE AAKRKT GRFVI V
Sbjct: 181 TYKKGFPSWLSGGDRRLLQSSSTKVDLVVAQDGSGNHTTVGAALEEAAKRKTNGRFVIQV 240

Query: 280 KRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIARGI 339
           KRGVY+ENLEIG+KMKNIML+GDGMRFT ITG+RSV GGSTTF+SATVAVTGEGFIARGI
Sbjct: 241 KRGVYQENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIARGI 300

Query: 340 TFRNTAGPENHQAVALRSGADFSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFIFG 399
           TFRNTAGPENHQAVALRSGAD SVFYRC FEGYQDTLYVHSQRQFYKECYIYGTVDFIFG
Sbjct: 301 TFRNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFG 360

Query: 400 NAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVESTVKT 459
           NAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK VESTVKT
Sbjct: 361 NAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKT 420

Query: 460 YLGRPWKEYSRTVFMQTYLDSLVDPAGWLEWDGEFALNTLYYGEYKNMGPGSSTSQRVKW 519
           YLGRPWKEYSRTVFM+TYLDSLVDP GWLEWDG+FAL+TLYYGEY N+GP S TSQRV W
Sbjct: 421 YLGRPWKEYSRTVFMKTYLDSLVDPTGWLEWDGDFALDTLYYGEYNNIGPRSPTSQRVNW 480

Query: 520 RGYHIVTNSTEASKFTVQNFIAGQSWLPGTEVPFTPGL 557
           +GY ++ NSTEAS+FTVQNFIAGQSWLP TEVPFTPGL
Sbjct: 481 KGYRVIRNSTEASEFTVQNFIAGQSWLPDTEVPFTPGL 518

[Close]

BLAST of MC10g_new0038 vs. NCBI nr
Match: XP_004148139.2 (pectinesterase 2 [Cucumis sativus] >KGN57272.1 hypothetical protein Csa_010597 [Cucumis sativus])

HSP 1 Score: 920 bits (2379), Expect = 0.0
Identity = 465/547 (85.01%), Postives = 494/547 (90.31%), Query Frame = 0


Query: 17  LLP--YITKPEVACLASSKQEPA-LLMEKSFGVLFFLLFFSLSSP-AFSKHASNGIDWWC 76
           LLP  YI K + + LASS  E A L M  SFGV  FL FF LSS  AFSK ASNGIDWWC
Sbjct: 25  LLPLYYIIKHKRSILASSNIEMAGLSMGTSFGVFIFLHFFLLSSSSAFSKPASNGIDWWC 84

Query: 77  SKTTYPETCKYFFNHGSNAAPKDMSEFKKMVTKFAMERALRAESHAKGVGSKCRNEKEKA 136
            KTTYPETCKYFFNHG+ + PKDM++FKKMV +FAMERAL AESH KGVGSKCRN KEKA
Sbjct: 85  KKTTYPETCKYFFNHGTKSPPKDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKA 144

Query: 137 AWADCLELYQNTILQLNHTLDSSSKCTEFDIQTWLSTALTNLETCYTGFAEFNVSDHVLP 196
           AWADCL+LYQNTILQLN TLDSS+K TEFDIQTWLSTALTNLETC TGFAE NVSD++LP
Sbjct: 145 AWADCLKLYQNTILQLNQTLDSSTKSTEFDIQTWLSTALTNLETCRTGFAELNVSDYILP 204

Query: 197 LIMSNNVTKLISNSLAINNASAWPG--KETYKEGFPSWLLGGDRRLLQSSSVKTDLVVAQ 256
           LIMS+NVT+LISNSLAINNASA  G  KETYK+GFPSWL GGDRRLLQSSS K DLVVAQ
Sbjct: 205 LIMSDNVTELISNSLAINNASAGVGNGKETYKKGFPSWLSGGDRRLLQSSSTKVDLVVAQ 264

Query: 257 DGSGDHRTVGAALEAAAKRKTGGRFVIHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIIT 316
           DGSG++ TVGAALE AAKRKT GRFVI VKRGVY ENLEIG+KMKNIML+GDGMRFT IT
Sbjct: 265 DGSGNYTTVGAALEEAAKRKTSGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFIT 324

Query: 317 GSRSVRGGSTTFHSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGADFSVFYRCGFE 376
           G+RSV GGSTTF+SATVAVTGEGFIARGITFRNTAGPENHQAVALRSGAD SVFYRC FE
Sbjct: 325 GNRSVGGGSTTFNSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGADLSVFYRCAFE 384

Query: 377 GYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARRPMNGQKNAVTAQGRTD 436
           GYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYAR+PMNGQKNAVTAQGRTD
Sbjct: 385 GYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTD 444

Query: 437 PNQNTGISIHNSRVMATDDLKQVESTVKTYLGRPWKEYSRTVFMQTYLDSLVDPAGWLEW 496
           PNQNTGISIHNSRVMATDDLK VESTVKTYLGRPWKEYSRTVFM+TY+DSLVDPAGWLEW
Sbjct: 445 PNQNTGISIHNSRVMATDDLKPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPAGWLEW 504

Query: 497 DGEFALNTLYYGEYKNMGPGSSTSQRVKWRGYHIVTNSTEASKFTVQNFIAGQSWLPGTE 556
           DG+FALNTLYYGEY N+GPGS  SQRVKW+GYH++TN TEAS+FTVQNFIAGQSWLP TE
Sbjct: 505 DGDFALNTLYYGEYNNIGPGSPISQRVKWKGYHVITNLTEASEFTVQNFIAGQSWLPDTE 564

[Close]

BLAST of MC10g_new0038 vs. NCBI nr
Match: KAA0067674.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYK23678.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 907 bits (2345), Expect = 0.0
Identity = 448/521 (85.99%), Postives = 476/521 (91.36%), Query Frame = 0


Query: 40  MEKSF-GVLFFLLFFSLSSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSNAAPKDMSE 99
           ME S  GV  FL FF LSS AFSK ASNGIDWWC KTTYPETCKYFFNHG+ + PKDM++
Sbjct: 6   METSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPPKDMTD 65

Query: 100 FKKMVTKFAMERALRAESHAKGVGSKCRNEKEKAAWADCLELYQNTILQLNHTLDSSSKC 159
           FKKMV +FAMERAL AESH KGVGSKCRN KEKAAWADCL+LYQNTILQLN TLDSS+KC
Sbjct: 66  FKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTKC 125

Query: 160 TEFDIQTWLSTALTNLETCYTGFAEFNVSDHVLPLIMSNNVTKLISNSLAINNASAWPG- 219
           TEFDIQTWLSTALTNLETC TGFAE NV D++ PLIMS+NVTKLISNSLAINNASA  G 
Sbjct: 126 TEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAGVGN 185

Query: 220 -KETYKEGFPSWLLGGDRRLLQSSSVKTDLVVAQDGSGDHRTVGAALEAAAKRKTGGRFV 279
            K TYK+GFPSWL GGDRRLLQS S K DLVVAQDGSG++ TVGAALE AAKRKT GRFV
Sbjct: 186 GKVTYKKGFPSWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGRFV 245

Query: 280 IHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIA 339
           I VKRGVY ENLEIG+KMKNIML+GDGMRFT ITG+RSV GGSTTF+SATVAVTGEGFIA
Sbjct: 246 IQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIA 305

Query: 340 RGITFRNTAGPENHQAVALRSGADFSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVDF 399
           RGITF+NTAGPENHQAVALRSGAD SVFYRC FEGYQDTLYVHSQRQFYKECYIYGTVDF
Sbjct: 306 RGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDF 365

Query: 400 IFGNAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVEST 459
           IFGNAAVVLQNCMIYAR+PMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK VEST
Sbjct: 366 IFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVEST 425

Query: 460 VKTYLGRPWKEYSRTVFMQTYLDSLVDPAGWLEWDGEFALNTLYYGEYKNMGPGSSTSQR 519
           VKTYLGRPWKEYSRTVFM+TY+DSLVDP GWLEWDG+FALNTLYYGEY N+GPGS  SQR
Sbjct: 426 VKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGSPISQR 485

Query: 520 VKWRGYHIVTNSTEASKFTVQNFIAGQSWLPGTEVPFTPGL 557
           VKW+GYH+++NSTEAS+FTVQNFIAGQSWLP TEVPFTPGL
Sbjct: 486 VKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 526

[Close]

BLAST of MC10g_new0038 vs. NCBI nr
Match: XP_022989127.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 906 bits (2342), Expect = 0.0
Identity = 447/547 (81.72%), Postives = 486/547 (88.85%), Query Frame = 0


Query: 14  NLALLPYITKPEVACLA--SSKQEPALLMEKSF-GVLFFLLFFSLSSPAFSKHASNGIDW 73
             ALLPYITKP  A LA  ++  E  L ME+S  GVLFFL FF  SS AFSKHASNGI W
Sbjct: 4   QFALLPYITKPLRATLALINTYNELGLSMERSILGVLFFLHFFLPSSSAFSKHASNGIHW 63

Query: 74  WCSKTTYPETCKYFFNHGSNAAPKDMSEFKKMVTKFAMERALRAESHAKGVGSKCRNEKE 133
           WC+KTTYPETCKYF+ + +N  PKDMSEFKKM  KFAM+ AL  E H K VGS+CRN KE
Sbjct: 64  WCTKTTYPETCKYFYGNANNPPPKDMSEFKKMAIKFAMDGALSVERHTKRVGSRCRNHKE 123

Query: 134 KAAWADCLELYQNTILQLNHTLDSSSKCTEFDIQTWLSTALTNLETCYTGFAEFNVSDHV 193
           + AWADCL+LYQNTI QL  TL+SS+K TEFDIQTWLSTALTN+ETC TGFAE NV D+V
Sbjct: 124 RVAWADCLKLYQNTIFQLKRTLESSTKSTEFDIQTWLSTALTNVETCRTGFAELNVVDNV 183

Query: 194 LPLIMSNNVTKLISNSLAINNASAWPGKETYKEGFPSWLLGGDRRLLQSSSVKTDLVVAQ 253
           LPLI S NVTKLISNSLA+NNAS W GKETYK+GFPSWL GGDR+LLQSSS+  DLVVAQ
Sbjct: 184 LPLITSKNVTKLISNSLAVNNASTWQGKETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQ 243

Query: 254 DGSGDHRTVGAALEAAAKRKTGGRFVIHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIIT 313
           DGSGDH+TV AALE AAKR T GRFVI VKRGVYEENLEIGNKMKNIMLVGDGMRFT IT
Sbjct: 244 DGSGDHKTVAAALEEAAKRNTDGRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTFIT 303

Query: 314 GSRSVRGGSTTFHSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGADFSVFYRCGFE 373
            SRSV GGSTTF+SATVAVTGEGFIA+GITFRNTAGPENHQAVALRSGAD SVFYRC FE
Sbjct: 304 SSRSVGGGSTTFNSATVAVTGEGFIAQGITFRNTAGPENHQAVALRSGADHSVFYRCAFE 363

Query: 374 GYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARRPMNGQKNAVTAQGRTD 433
           GYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYAR+PMNGQKNA+TAQGRTD
Sbjct: 364 GYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGRTD 423

Query: 434 PNQNTGISIHNSRVMATDDLKQVESTVKTYLGRPWKEYSRTVFMQTYLDSLVDPAGWLEW 493
           PNQNTGISIHNSRVMATDDLKQVE TVKT+LGRPWKEYSRTVF++TYLDSLVDPAGWLEW
Sbjct: 424 PNQNTGISIHNSRVMATDDLKQVEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEW 483

Query: 494 DGEFALNTLYYGEYKNMGPGSSTSQRVKWRGYHIVTNSTEASKFTVQNFIAGQSWLPGTE 553
           +G+FALNTLYYGEYKN+GP SST QRV+W+GYH+++NSTEAS+F+VQNFI+GQSWLPGTE
Sbjct: 484 NGDFALNTLYYGEYKNIGPRSSTDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPGTE 543

Query: 554 VPFTPGL 557
           VPF PG+
Sbjct: 544 VPFIPGI 550

[Close]

BLAST of MC10g_new0038 vs. ExPASy TrEMBL
Match: A0A6J1CH86 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111011631 PE=3 SV=1)

HSP 1 Score: 1132 bits (2927), Expect = 0.0
Identity = 557/557 (100.00%), Postives = 557/557 (100.00%), Query Frame = 0


Query: 1   MFKPSQPVSPFHPNLALLPYITKPEVACLASSKQEPALLMEKSFGVLFFLLFFSLSSPAF 60
           MFKPSQPVSPFHPNLALLPYITKPEVACLASSKQEPALLMEKSFGVLFFLLFFSLSSPAF
Sbjct: 1   MFKPSQPVSPFHPNLALLPYITKPEVACLASSKQEPALLMEKSFGVLFFLLFFSLSSPAF 60

Query: 61  SKHASNGIDWWCSKTTYPETCKYFFNHGSNAAPKDMSEFKKMVTKFAMERALRAESHAKG 120
           SKHASNGIDWWCSKTTYPETCKYFFNHGSNAAPKDMSEFKKMVTKFAMERALRAESHAKG
Sbjct: 61  SKHASNGIDWWCSKTTYPETCKYFFNHGSNAAPKDMSEFKKMVTKFAMERALRAESHAKG 120

Query: 121 VGSKCRNEKEKAAWADCLELYQNTILQLNHTLDSSSKCTEFDIQTWLSTALTNLETCYTG 180
           VGSKCRNEKEKAAWADCLELYQNTILQLNHTLDSSSKCTEFDIQTWLSTALTNLETCYTG
Sbjct: 121 VGSKCRNEKEKAAWADCLELYQNTILQLNHTLDSSSKCTEFDIQTWLSTALTNLETCYTG 180

Query: 181 FAEFNVSDHVLPLIMSNNVTKLISNSLAINNASAWPGKETYKEGFPSWLLGGDRRLLQSS 240
           FAEFNVSDHVLPLIMSNNVTKLISNSLAINNASAWPGKETYKEGFPSWLLGGDRRLLQSS
Sbjct: 181 FAEFNVSDHVLPLIMSNNVTKLISNSLAINNASAWPGKETYKEGFPSWLLGGDRRLLQSS 240

Query: 241 SVKTDLVVAQDGSGDHRTVGAALEAAAKRKTGGRFVIHVKRGVYEENLEIGNKMKNIMLV 300
           SVKTDLVVAQDGSGDHRTVGAALEAAAKRKTGGRFVIHVKRGVYEENLEIGNKMKNIMLV
Sbjct: 241 SVKTDLVVAQDGSGDHRTVGAALEAAAKRKTGGRFVIHVKRGVYEENLEIGNKMKNIMLV 300

Query: 301 GDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGAD 360
           GDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGAD
Sbjct: 301 GDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGAD 360

Query: 361 FSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARRPMNGQK 420
           FSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARRPMNGQK
Sbjct: 361 FSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARRPMNGQK 420

Query: 421 NAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVESTVKTYLGRPWKEYSRTVFMQTYLDS 480
           NAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVESTVKTYLGRPWKEYSRTVFMQTYLDS
Sbjct: 421 NAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVESTVKTYLGRPWKEYSRTVFMQTYLDS 480

Query: 481 LVDPAGWLEWDGEFALNTLYYGEYKNMGPGSSTSQRVKWRGYHIVTNSTEASKFTVQNFI 540
           LVDPAGWLEWDGEFALNTLYYGEYKNMGPGSSTSQRVKWRGYHIVTNSTEASKFTVQNFI
Sbjct: 481 LVDPAGWLEWDGEFALNTLYYGEYKNMGPGSSTSQRVKWRGYHIVTNSTEASKFTVQNFI 540

Query: 541 AGQSWLPGTEVPFTPGL 557
           AGQSWLPGTEVPFTPGL
Sbjct: 541 AGQSWLPGTEVPFTPGL 557

[Close]

BLAST of MC10g_new0038 vs. ExPASy TrEMBL
Match: A0A0A0L9G4 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G175720 PE=3 SV=1)

HSP 1 Score: 920 bits (2379), Expect = 0.0
Identity = 465/547 (85.01%), Postives = 494/547 (90.31%), Query Frame = 0


Query: 17  LLP--YITKPEVACLASSKQEPA-LLMEKSFGVLFFLLFFSLSSP-AFSKHASNGIDWWC 76
           LLP  YI K + + LASS  E A L M  SFGV  FL FF LSS  AFSK ASNGIDWWC
Sbjct: 25  LLPLYYIIKHKRSILASSNIEMAGLSMGTSFGVFIFLHFFLLSSSSAFSKPASNGIDWWC 84

Query: 77  SKTTYPETCKYFFNHGSNAAPKDMSEFKKMVTKFAMERALRAESHAKGVGSKCRNEKEKA 136
            KTTYPETCKYFFNHG+ + PKDM++FKKMV +FAMERAL AESH KGVGSKCRN KEKA
Sbjct: 85  KKTTYPETCKYFFNHGTKSPPKDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKA 144

Query: 137 AWADCLELYQNTILQLNHTLDSSSKCTEFDIQTWLSTALTNLETCYTGFAEFNVSDHVLP 196
           AWADCL+LYQNTILQLN TLDSS+K TEFDIQTWLSTALTNLETC TGFAE NVSD++LP
Sbjct: 145 AWADCLKLYQNTILQLNQTLDSSTKSTEFDIQTWLSTALTNLETCRTGFAELNVSDYILP 204

Query: 197 LIMSNNVTKLISNSLAINNASAWPG--KETYKEGFPSWLLGGDRRLLQSSSVKTDLVVAQ 256
           LIMS+NVT+LISNSLAINNASA  G  KETYK+GFPSWL GGDRRLLQSSS K DLVVAQ
Sbjct: 205 LIMSDNVTELISNSLAINNASAGVGNGKETYKKGFPSWLSGGDRRLLQSSSTKVDLVVAQ 264

Query: 257 DGSGDHRTVGAALEAAAKRKTGGRFVIHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIIT 316
           DGSG++ TVGAALE AAKRKT GRFVI VKRGVY ENLEIG+KMKNIML+GDGMRFT IT
Sbjct: 265 DGSGNYTTVGAALEEAAKRKTSGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFIT 324

Query: 317 GSRSVRGGSTTFHSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGADFSVFYRCGFE 376
           G+RSV GGSTTF+SATVAVTGEGFIARGITFRNTAGPENHQAVALRSGAD SVFYRC FE
Sbjct: 325 GNRSVGGGSTTFNSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGADLSVFYRCAFE 384

Query: 377 GYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARRPMNGQKNAVTAQGRTD 436
           GYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYAR+PMNGQKNAVTAQGRTD
Sbjct: 385 GYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTD 444

Query: 437 PNQNTGISIHNSRVMATDDLKQVESTVKTYLGRPWKEYSRTVFMQTYLDSLVDPAGWLEW 496
           PNQNTGISIHNSRVMATDDLK VESTVKTYLGRPWKEYSRTVFM+TY+DSLVDPAGWLEW
Sbjct: 445 PNQNTGISIHNSRVMATDDLKPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPAGWLEW 504

Query: 497 DGEFALNTLYYGEYKNMGPGSSTSQRVKWRGYHIVTNSTEASKFTVQNFIAGQSWLPGTE 556
           DG+FALNTLYYGEY N+GPGS  SQRVKW+GYH++TN TEAS+FTVQNFIAGQSWLP TE
Sbjct: 505 DGDFALNTLYYGEYNNIGPGSPISQRVKWKGYHVITNLTEASEFTVQNFIAGQSWLPDTE 564

[Close]

BLAST of MC10g_new0038 vs. ExPASy TrEMBL
Match: A0A5D3DJL7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00440 PE=3 SV=1)

HSP 1 Score: 907 bits (2345), Expect = 0.0
Identity = 448/521 (85.99%), Postives = 476/521 (91.36%), Query Frame = 0


Query: 40  MEKSF-GVLFFLLFFSLSSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSNAAPKDMSE 99
           ME S  GV  FL FF LSS AFSK ASNGIDWWC KTTYPETCKYFFNHG+ + PKDM++
Sbjct: 6   METSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPPKDMTD 65

Query: 100 FKKMVTKFAMERALRAESHAKGVGSKCRNEKEKAAWADCLELYQNTILQLNHTLDSSSKC 159
           FKKMV +FAMERAL AESH KGVGSKCRN KEKAAWADCL+LYQNTILQLN TLDSS+KC
Sbjct: 66  FKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTKC 125

Query: 160 TEFDIQTWLSTALTNLETCYTGFAEFNVSDHVLPLIMSNNVTKLISNSLAINNASAWPG- 219
           TEFDIQTWLSTALTNLETC TGFAE NV D++ PLIMS+NVTKLISNSLAINNASA  G 
Sbjct: 126 TEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAGVGN 185

Query: 220 -KETYKEGFPSWLLGGDRRLLQSSSVKTDLVVAQDGSGDHRTVGAALEAAAKRKTGGRFV 279
            K TYK+GFPSWL GGDRRLLQS S K DLVVAQDGSG++ TVGAALE AAKRKT GRFV
Sbjct: 186 GKVTYKKGFPSWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGRFV 245

Query: 280 IHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIA 339
           I VKRGVY ENLEIG+KMKNIML+GDGMRFT ITG+RSV GGSTTF+SATVAVTGEGFIA
Sbjct: 246 IQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIA 305

Query: 340 RGITFRNTAGPENHQAVALRSGADFSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVDF 399
           RGITF+NTAGPENHQAVALRSGAD SVFYRC FEGYQDTLYVHSQRQFYKECYIYGTVDF
Sbjct: 306 RGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDF 365

Query: 400 IFGNAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVEST 459
           IFGNAAVVLQNCMIYAR+PMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK VEST
Sbjct: 366 IFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVEST 425

Query: 460 VKTYLGRPWKEYSRTVFMQTYLDSLVDPAGWLEWDGEFALNTLYYGEYKNMGPGSSTSQR 519
           VKTYLGRPWKEYSRTVFM+TY+DSLVDP GWLEWDG+FALNTLYYGEY N+GPGS  SQR
Sbjct: 426 VKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGSPISQR 485

Query: 520 VKWRGYHIVTNSTEASKFTVQNFIAGQSWLPGTEVPFTPGL 557
           VKW+GYH+++NSTEAS+FTVQNFIAGQSWLP TEVPFTPGL
Sbjct: 486 VKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 526

[Close]

BLAST of MC10g_new0038 vs. ExPASy TrEMBL
Match: A0A6J1JEY1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111486289 PE=3 SV=1)

HSP 1 Score: 906 bits (2342), Expect = 0.0
Identity = 447/547 (81.72%), Postives = 486/547 (88.85%), Query Frame = 0


Query: 14  NLALLPYITKPEVACLA--SSKQEPALLMEKSF-GVLFFLLFFSLSSPAFSKHASNGIDW 73
             ALLPYITKP  A LA  ++  E  L ME+S  GVLFFL FF  SS AFSKHASNGI W
Sbjct: 4   QFALLPYITKPLRATLALINTYNELGLSMERSILGVLFFLHFFLPSSSAFSKHASNGIHW 63

Query: 74  WCSKTTYPETCKYFFNHGSNAAPKDMSEFKKMVTKFAMERALRAESHAKGVGSKCRNEKE 133
           WC+KTTYPETCKYF+ + +N  PKDMSEFKKM  KFAM+ AL  E H K VGS+CRN KE
Sbjct: 64  WCTKTTYPETCKYFYGNANNPPPKDMSEFKKMAIKFAMDGALSVERHTKRVGSRCRNHKE 123

Query: 134 KAAWADCLELYQNTILQLNHTLDSSSKCTEFDIQTWLSTALTNLETCYTGFAEFNVSDHV 193
           + AWADCL+LYQNTI QL  TL+SS+K TEFDIQTWLSTALTN+ETC TGFAE NV D+V
Sbjct: 124 RVAWADCLKLYQNTIFQLKRTLESSTKSTEFDIQTWLSTALTNVETCRTGFAELNVVDNV 183

Query: 194 LPLIMSNNVTKLISNSLAINNASAWPGKETYKEGFPSWLLGGDRRLLQSSSVKTDLVVAQ 253
           LPLI S NVTKLISNSLA+NNAS W GKETYK+GFPSWL GGDR+LLQSSS+  DLVVAQ
Sbjct: 184 LPLITSKNVTKLISNSLAVNNASTWQGKETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQ 243

Query: 254 DGSGDHRTVGAALEAAAKRKTGGRFVIHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIIT 313
           DGSGDH+TV AALE AAKR T GRFVI VKRGVYEENLEIGNKMKNIMLVGDGMRFT IT
Sbjct: 244 DGSGDHKTVAAALEEAAKRNTDGRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTFIT 303

Query: 314 GSRSVRGGSTTFHSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGADFSVFYRCGFE 373
            SRSV GGSTTF+SATVAVTGEGFIA+GITFRNTAGPENHQAVALRSGAD SVFYRC FE
Sbjct: 304 SSRSVGGGSTTFNSATVAVTGEGFIAQGITFRNTAGPENHQAVALRSGADHSVFYRCAFE 363

Query: 374 GYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARRPMNGQKNAVTAQGRTD 433
           GYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYAR+PMNGQKNA+TAQGRTD
Sbjct: 364 GYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGRTD 423

Query: 434 PNQNTGISIHNSRVMATDDLKQVESTVKTYLGRPWKEYSRTVFMQTYLDSLVDPAGWLEW 493
           PNQNTGISIHNSRVMATDDLKQVE TVKT+LGRPWKEYSRTVF++TYLDSLVDPAGWLEW
Sbjct: 424 PNQNTGISIHNSRVMATDDLKQVEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEW 483

Query: 494 DGEFALNTLYYGEYKNMGPGSSTSQRVKWRGYHIVTNSTEASKFTVQNFIAGQSWLPGTE 553
           +G+FALNTLYYGEYKN+GP SST QRV+W+GYH+++NSTEAS+F+VQNFI+GQSWLPGTE
Sbjct: 484 NGDFALNTLYYGEYKNIGPRSSTDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPGTE 543

Query: 554 VPFTPGL 557
           VPF PG+
Sbjct: 544 VPFIPGI 550

[Close]

BLAST of MC10g_new0038 vs. ExPASy TrEMBL
Match: A0A1S3AYJ9 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483986 PE=3 SV=1)

HSP 1 Score: 903 bits (2334), Expect = 0.0
Identity = 447/521 (85.80%), Postives = 476/521 (91.36%), Query Frame = 0


Query: 40  MEKSF-GVLFFLLFFSLSSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSNAAPKDMSE 99
           ME S  GV  FL FF LSS AFSK ASNGIDWWC KTTYPETCKYFFNHG+ + PKDM++
Sbjct: 6   METSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPPKDMTD 65

Query: 100 FKKMVTKFAMERALRAESHAKGVGSKCRNEKEKAAWADCLELYQNTILQLNHTLDSSSKC 159
           FKKMV +FAMERAL AESH KGVGSKCRN KEKAAWADCL+LYQNTILQLN TLDSS+K 
Sbjct: 66  FKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTKR 125

Query: 160 TEFDIQTWLSTALTNLETCYTGFAEFNVSDHVLPLIMSNNVTKLISNSLAINNASA--WP 219
           TEFDIQTWLSTALTNLETC TGFAE NV D++ PLIMS+NVTKLISNSLAINNASA    
Sbjct: 126 TEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAAVGN 185

Query: 220 GKETYKEGFPSWLLGGDRRLLQSSSVKTDLVVAQDGSGDHRTVGAALEAAAKRKTGGRFV 279
           GK TYK+GFPSWL GGDRRLLQS+S K DLVVAQDGSG++ TVGAALE AAKRKT GRFV
Sbjct: 186 GKVTYKKGFPSWLSGGDRRLLQSASTKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGRFV 245

Query: 280 IHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIA 339
           I VKRGVY ENLEIG+KMKNIML+GDGMRFT ITG+RSV GGSTTF+SATVAVTGEGFIA
Sbjct: 246 IQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIA 305

Query: 340 RGITFRNTAGPENHQAVALRSGADFSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVDF 399
           RGITF+NTAGPENHQAVALRSGAD SVFYRC FEGYQDTLYVHSQRQFYKECYIYGTVDF
Sbjct: 306 RGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDF 365

Query: 400 IFGNAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVEST 459
           IFGNAAVVLQNCMIYAR+PMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK VEST
Sbjct: 366 IFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVEST 425

Query: 460 VKTYLGRPWKEYSRTVFMQTYLDSLVDPAGWLEWDGEFALNTLYYGEYKNMGPGSSTSQR 519
           VKTYLGRPWKEYSRTVFM+TY+DSLVDP GWLEWDG+FALNTLYYGEY N+GPGS  SQR
Sbjct: 426 VKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGSPISQR 485

Query: 520 VKWRGYHIVTNSTEASKFTVQNFIAGQSWLPGTEVPFTPGL 557
           VKW+GYH+++NSTEAS+FTVQNFIAGQSWLP TEVPFTPGL
Sbjct: 486 VKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 526

[Close]

BLAST of MC10g_new0038 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 602.4 bits (1552), Expect = 3.6e-172
Identity = 303/513 (59.06%), Postives = 386/513 (75.24%), Query Frame = 0


Query: 46  VLFFLLFFSLSSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSNAAP-KDMSEFKKMVT 105
           ++ F++   L +   S +    +  WCS+T  P+ C+YF  H SN  P K  SEF K+  
Sbjct: 8   IINFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISM 67

Query: 106 KFAMERALRAESHAKGVGSKCRNEKEKAAWADCLELYQNTILQLNHTLDSSSKCTEFDIQ 165
           K  ++RA+ A++HA  +G KCR+ +EKAAW DC++LY  T+ ++N T+D + KC++ D Q
Sbjct: 68  KLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQ 127

Query: 166 TWLSTALTNLETCYTGFAEFNVSDHVLPLIMSNNVTKLISNSLAINNASAWPGKETYKEG 225
           TWLSTALTNL+TC  GF E  V+D VLPL MSNNV+ L+ N+LAIN    +      K+G
Sbjct: 128 TWLSTALTNLDTCRAGFLELGVTDIVLPL-MSNNVSNLLCNTLAINKV-PFNYTPPEKDG 187

Query: 226 FPSWLLGGDRRLLQSSSVKTDLVVAQDGSGDHRTVGAALEAAAKRKTGGRFVIHVKRGVY 285
           FPSW+  GDR+LLQSS+ K + VVA+DGSG+ +T+  A++AA+     GRFVI+VK+GVY
Sbjct: 188 FPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAAS---GSGRFVIYVKQGVY 247

Query: 286 EENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIARGITFRNT 345
            ENLEI  + KN+ML GDG+  TIITGS+SV GG+TTF+SATVA  G+GFIARGITFRNT
Sbjct: 248 SENLEI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNT 307

Query: 346 AGPENHQAVALRSGADFSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVV 405
           AG  N QAVALRSG+D SVFY+C FE YQDTLYVHS RQFY++C +YGTVDFIFGNAA V
Sbjct: 308 AGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAV 367

Query: 406 LQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVESTVKTYLGRP 465
           LQNC I+ARRP   + N +TAQGR+DPNQNTGI IHNSRV A  DL+ V  + KTYLGRP
Sbjct: 368 LQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRP 427

Query: 466 WKEYSRTVFMQTYLDSLVDPAGWLEWDGEFALNTLYYGEYKNMGPGSSTSQRVKWRGYHI 525
           W++YSRTVFM+T LDSL+DP GWLEWDG FAL TL+Y E++N GPG+STS RV W G+ +
Sbjct: 428 WRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRV 487

Query: 526 VTNSTEASKFTVQNFIAGQSWLPGTEVPFTPGL 558
           + +++EASKFTV  F+AG SW+P + VPFT GL
Sbjct: 488 LGSASEASKFTVGTFLAGGSWIP-SSVPFTSGL 511

[Close]

BLAST of MC10g_new0038 vs. TAIR 10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 568.5 bits (1464), Expect = 5.7e-162
Identity = 289/537 (53.82%), Postives = 371/537 (69.09%), Query Frame = 0


Query: 39  LMEKSFGVLF---FLLFFSLSSPAFSKHASNGIDWWCSKTTYPETCK-YFFNHGSNAAPK 98
           +M +   +LF    LL      P  +    N  D WC KT YP+ CK YF NH     P 
Sbjct: 3   MMMQKLSILFLHLILLVLLCVHPLTTVADRNSTD-WCDKTPYPDPCKCYFKNHNGFQQPT 62

Query: 99  DMSEFKKMVTKFAMERALRAESHAKGVGSKCRNEKEKAAWADCLELYQNTILQLNHTL-- 158
            +SEF+ M+ + AM+RA+ A +     G  C + K++A  ADC++LY +TI+QLN TL  
Sbjct: 63  QLSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHG 122

Query: 159 -----DSSSKCTEFDIQTWLSTALTNLETCYTGFAEFNVSDHVLPLIMSNNVTKLISNSL 218
                 ++  CT+FD QTWLSTALTN ETC  G ++ NV+D + P++ +  ++ LISN L
Sbjct: 123 VSPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCL 182

Query: 219 AINNASAWPGKE----TYKEGFPSWLLGGDRRLLQSSSVKTDLVVAQDGSGDHRTVGAAL 278
           A+N A    G +      ++GFP+WL   D+RLL+  +V+ +LVVA+DGSG   TV AA+
Sbjct: 183 AVNGALLTAGNKGNTTANQKGFPTWLSRKDKRLLR--AVRANLVVAKDGSGHFNTVQAAI 242

Query: 279 EAAAKRK-TGGRFVIHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTF 338
           + A +RK T GRFVI+VKRG+Y+EN+ +     +IMLVGDGMR TIITG RSV+GG TT+
Sbjct: 243 DVAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTY 302

Query: 339 HSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGADFSVFYRCGFEGYQDTLYVHSQR 398
           +SAT  + G  FIA+GITFRNTAGP   QAVALRS +D S+FY+C  EGYQDTL VHSQR
Sbjct: 303 NSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQR 362

Query: 399 QFYKECYIYGTVDFIFGNAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNS 458
           QFY+ECYIYGTVDFIFGNAA V QNC+I  RRP+ GQ N +TAQGR DP QNTGISIHNS
Sbjct: 363 QFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNS 422

Query: 459 RVMATDDLKQVESTVKTYLGRPWKEYSRTVFMQTYLDSLVDPAGWLEW--DGEFALNTLY 518
           R++   DLK V  TVKTY+GRPW ++SRTV +QTYLD++V P GW  W     F L+TL+
Sbjct: 423 RILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLF 482

Query: 519 YGEYKNMGPGSSTSQRVKWRGYHIVTNSTEASKFTVQNFIAGQSWLPGTEVPFTPGL 558
           Y EYKN GP SST  RV W+G+H++  +++AS FTV  FIAG +WLP T +PFT GL
Sbjct: 483 YAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

[Close]

BLAST of MC10g_new0038 vs. TAIR 10
Match: AT5G51500.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 562.4 bits (1448), Expect = 4.1e-160
Identity = 280/506 (55.34%), Postives = 356/506 (70.36%), Query Frame = 0


Query: 65  SNGIDWWCSKTTYPETCK-YFFNHGSNAAPKDMSEFKKMVTKFAMERALRAESHAKGVGS 124
           S  ID WC KT YP  CK YF  H     P  +SEF+ ++ + AM+RA+ A         
Sbjct: 35  STNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQISEFRVLLVEAAMDRAVSAWDKLTNSSK 94

Query: 125 KCRNEKEKAAWADCLELYQNTILQLNHTLDSSS-----KCTEFDIQTWLSTALTNLETCY 184
            C + K++A  ADC+ LY +T++QLN TL   S     +CT+FD QTWLSTALTN ETC 
Sbjct: 95  NCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKTGRRCTDFDAQTWLSTALTNTETCR 154

Query: 185 TGFAEFNVSDHVLPLIMSNNVTKLISNSLAINNASAWPGKETY----KEGFPSWLLGGDR 244
            G ++ NVSD   P++ +  ++ LISN LA+N A    GK        +GFP+W+   +R
Sbjct: 155 RGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALLTAGKNDSTTGDSKGFPTWVSRKER 214

Query: 245 RLLQSSSVKTDLVVAQDGSGDHRTVGAALEAAAKRK-TGGRFVIHVKRGVYEENLEIGNK 304
           RLLQ  SV+ +LVVA+DGSG  +TV AA++ A +RK T GRFVI+VKRG+Y+ENL +   
Sbjct: 215 RLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRKVTSGRFVIYVKRGIYQENLNVRLN 274

Query: 305 MKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIARGITFRNTAGPENHQAV 364
             NIMLVGDGMR+TIITG RSV+GG TT+ SAT  + G  FIA+GI F+NTAGP   QAV
Sbjct: 275 NDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGIEGLHFIAKGIAFQNTAGPAKGQAV 334

Query: 365 ALRSGADFSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYAR 424
           ALRS +D S+FYRC  EGYQDTL VHSQRQFY+ECYIYGTVDFIFGNAAVV QNC+I  R
Sbjct: 335 ALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYRECYIYGTVDFIFGNAAVVFQNCIILPR 394

Query: 425 RPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVESTVKTYLGRPWKEYSRTVF 484
            P+ GQ N +TAQGRTD  QNTGISIHNS ++   DLK V  +VKTY+GRPW  YSRTV 
Sbjct: 395 LPLKGQANVITAQGRTDLFQNTGISIHNSIIIPAPDLKPVVRSVKTYMGRPWMMYSRTVV 454

Query: 485 MQTYLDSLVDPAGWLEW--DGEFALNTLYYGEYKNMGPGSSTSQRVKWRGYHIVTNSTEA 544
           ++TY+DS+V P GW  W     + L+TL+Y EYKN+GP SST  RV+W+G+H+++ +++A
Sbjct: 455 LKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYKNIGPASSTRWRVRWKGFHVLSKASDA 514

Query: 545 SKFTVQNFIAGQSWLPGTEVPFTPGL 558
           S F+V  FIAG +WLPG+ +PFT  L
Sbjct: 515 SAFSVGKFIAGTAWLPGSGIPFTSEL 540

[Close]

BLAST of MC10g_new0038 vs. TAIR 10
Match: AT3G47400.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 552.4 bits (1422), Expect = 4.3e-157
Identity = 279/522 (53.45%), Postives = 357/522 (68.39%), Query Frame = 0


Query: 57  SPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSN-----AAPKDMSEFKKMVTKFAMERA 116
           SP  +   S  + WWC+KT + ETC Y+F   S        P+  SEF +M+ K A+++A
Sbjct: 73  SPINNGSVSGDMTWWCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQA 132

Query: 117 LRAESHAKGVGSKCRNEKEKAAWADCLELYQNTILQLNHTL-------DSSSKCTEFDIQ 176
           +   S     G  C N + KAAW+DC+ L+QNT+ QLN TL        S  KCT+FD Q
Sbjct: 133 VITHSQTVKFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQ 192

Query: 177 TWLSTALTNLETCYTGFAEFNVSDHVLPLIMSNNVTKLISNSLAINNASAWPGKET---- 236
           TWLSTA TN+ETC +G  + NVSD V+P+I + N++ LI N LA+N         T    
Sbjct: 193 TWLSTAQTNIETCRSGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTAN 252

Query: 237 YKEGFPSWLLGGDRRLLQSSSV---KTDLVVAQDGSGDHRTVGAALEAAAKRKTGGRFVI 296
           +KE FPSW+   +RRLL S+S+      LVVAQD SG  R++ AA+  AA+R+   RFVI
Sbjct: 253 HKEYFPSWVSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVI 312

Query: 297 HVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIAR 356
           +VK+GVY EN+++GN   NIMLVGDG R TIIT  RSV+ G TT++SAT    G+ F+A+
Sbjct: 313 YVKKGVYRENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAK 372

Query: 357 GITFRNTAGPENHQAVALRSGADFSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFI 416
            +TF NTAGP   QAVA+RS +D SVFYR G  G+QDTLY+HSQRQF++ECYI GT+DFI
Sbjct: 373 DMTFINTAGPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFI 432

Query: 417 FGNAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVESTV 476
           FGNAAVV QNCMI  RRP++GQ N +TAQGR DP QNTGI+IH+SR++A  DLK V    
Sbjct: 433 FGNAAVVFQNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAY 492

Query: 477 KTYLGRPWKEYSRTVFMQTYLDSLVDPAGWLEW--DGEFALNTLYYGEYKNMGPGSSTSQ 536
           KTYLGRPW+ YSR   M+TY+D+ + P GW  W     FALNT++YGEYKN GPGSST  
Sbjct: 493 KTYLGRPWQAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRW 552

Query: 537 RVKWRGYHIVTNSTEASKFTVQNFIAGQSWLPGTEVPFTPGL 558
           RV+W+G+H +T++  AS+FTV + IAG SWLP T VPF  GL
Sbjct: 553 RVRWKGFHAITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

[Close]

BLAST of MC10g_new0038 vs. TAIR 10
Match: AT1G23200.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 452.6 bits (1163), Expect = 4.6e-127
Identity = 251/529 (47.45%), Postives = 326/529 (61.63%), Query Frame = 0


Query: 72  CSKTTYPETCKYFFNHGSNAAPKDMSE------FKKMVTKFAMERALRAESHAKGVGSKC 131
           C +T YP  C +   H SN+  K + +      F  +V    M++A++       +    
Sbjct: 38  CKQTPYPSVCDH---HMSNSPLKTLDDQTDGFTFHDLVVSSTMDQAVQLHRLVSSLKQHH 97

Query: 132 RNEKE-KAAWADCLELYQNTILQLNHTLDSSSK-CTEFDIQTWLSTALTNLETCYTGFAE 191
              K   +A  DCLELY++TI QLNH+  S  +  +  D QT LS A+ N +TC  GF +
Sbjct: 98  SLHKHATSALFDCLELYEDTIDQLNHSRRSYGQYSSPHDRQTSLSAAIANQDTCRNGFRD 157

Query: 192 FNVS---DHVLPLIMSNNVTKLISNSLAINNASA--------WPGKETYK---------- 251
           F ++       P+    N+TK ISNSLA+  A+A        +P     K          
Sbjct: 158 FKLTSSYSKYFPVQFHRNLTKSISNSLAVTKAAAEAEAVAEKYPSTGFTKFSKQRSSAGG 217

Query: 252 -----------EGFPSWLLGGDRRLLQSS--SVKTDLVVAQDGSGDHRTVGAALEAAAK- 311
                      E FPSW    DR+LL+ S  + K DLVVA+DGSG + ++  A+ AAAK 
Sbjct: 218 GSHRRLLLFSDEKFPSWFPLSDRKLLEDSKTTAKADLVVAKDGSGHYTSIQQAVNAAAKL 277

Query: 312 RKTGGRFVIHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVA 371
            +   R VI+VK GVY EN+ I   +KN+M++GDG+  TI+TG+R+V+ G+TTF SAT A
Sbjct: 278 PRRNQRLVIYVKAGVYRENVVIKKSIKNVMVIGDGIDSTIVTGNRNVQDGTTTFRSATFA 337

Query: 372 VTGEGFIARGITFRNTAGPENHQAVALRSGADFSVFYRCGFEGYQDTLYVHSQRQFYKEC 431
           V+G GFIA+GITF NTAGPE HQAVALRS +DFSVFY C F+GYQDTLY+HS RQF + C
Sbjct: 338 VSGNGFIAQGITFENTAGPEKHQAVALRSSSDFSVFYACSFKGYQDTLYLHSSRQFLRNC 397

Query: 432 YIYGTVDFIFGNAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATD 491
            IYGTVDFIFG+A  +LQNC IYAR+PM+GQKN +TAQ R +P++ TG  I +S V    
Sbjct: 398 NIYGTVDFIFGDATAILQNCNIYARKPMSGQKNTITAQSRKEPDETTGFVIQSSTVATAS 457

Query: 492 DLKQVESTVKTYLGRPWKEYSRTVFMQTYLDSLVDPAGWLEWDGEFALNTLYYGEYKNMG 551
           +         TYLGRPW+ +SRTVFM+  L +LV PAGWL W G FAL+TLYYGEY N G
Sbjct: 458 E---------TYLGRPWRSHSRTVFMKCNLGALVSPAGWLPWSGSFALSTLYYGEYGNTG 517

Query: 552 PGSSTSQRVKWRGYHIVTNSTEASKFTVQNFIAGQSWLPGTEVPFTPGL 558
            G+S S RVKW GYH++   TEA KFTV+NF+ G  W+  T VP   GL
Sbjct: 518 AGASVSGRVKWPGYHVIKTVTEAEKFTVENFLDGNYWITATGVPVNDGL 554

The following BLAST results are available for this feature:

BLAST of MC10g_new0038 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04887	5.0e-179	61.89	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
O22149	5.0e-171	59.06	Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3...	[more]
Q9FHN5	8.1e-161	53.82	Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3...	[more]
Q9FHN4	5.8e-159	55.34	Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3...	[more]
Q9STY3	6.0e-156	53.45	Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of MC10g_new0038 vs. NCBI nr


Match Name	Identity	Description
XP_022141175.1	100.00	pectinesterase-like [Momordica charantia]	[more]
XP_038882414.1	88.03	pectinesterase-like [Benincasa hispida]	[more]
XP_004148139.2	85.01	pectinesterase 2 [Cucumis sativus] >KGN57272.1 hypothetical protein Csa_010597 [...	[more]
KAA0067674.1	85.99	pectinesterase-like [Cucumis melo var. makuwa] >TYK23678.1 pectinesterase-like [...	[more]
XP_022989127.1	81.72	pectinesterase-like [Cucurbita maxima]	[more]

BLAST of MC10g_new0038 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1CH86	100.00	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111011631 PE=3 SV=1	[more]
A0A0A0L9G4	85.01	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G175720 PE=3 SV=1	[more]
A0A5D3DJL7	85.99	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00440...	[more]
A0A6J1JEY1	81.72	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111486289 PE=3 SV=1	[more]
A0A1S3AYJ9	85.80	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483986 PE=3 SV=1	[more]

BLAST of MC10g_new0038 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45220.1	3.6e-172	59.06	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G51490.1	5.7e-162	53.82	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G51500.1	4.1e-160	55.34	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G47400.1	4.3e-157	53.45	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G23200.1	4.6e-127	47.45	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 63..209 e-value: 3.8E-32 score: 122.8
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 68..209 e-value: 1.3E-25 score: 90.5
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 40..211 e-value: 2.0E-27 score: 93.6
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 239..557 e-value: 7.9E-127 score: 425.0
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 245..542 e-value: 3.4E-147 score: 488.9
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 63..212 e-value: 9.7E-30 score: 105.6
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 66..211
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 46..556
None	No IPR available	PANTHER	PTHR31707:SF181	PECTINESTERASE	coord: 46..556
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 70..212 e-value: 1.45897E-34 score: 126.014
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 390..399
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 241..556

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g_new0038.1	MC10g_new0038.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity