MC10g1387 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g1387
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	short-chain dehydrogenase TIC 32, chloroplastic-like
Location	MC10: 17299154 .. 17300611 (-)
RNA-Seq Expression	MC10g1387
Synteny	MC10g1387

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTGAAATCCTGAAGTACCTCGTCGGTTCAGCCGGAGCAAGTGGCTACGGCTCCAAATCCACCGCCGTAGAGGTCACTCAATCCTGTCCTCATCTGTGCTCCGTCACCGCCATAATCACAGGTGATGATCTTCTGAATTCTGATTCTGTTCCATTAACATTACTCACTCAATCACATGCAAAATCATGCGCTCGCGAACAAATTGGGAGCAGGAGCGACGTCGGGAATCGGAGAGGAGACGGCGAGAGTCTTAGCCAAGCGCGGCGCCAGGATCGTCCTTCCGGCTCGCAACCTCAAAGCCGCCGAGGAAGCAAAGGCGCGCATCAAATCGGAATCGGAATCGGAATGTTCCGATCATTCACGAATCACGGTTATGGAACTCGATCTTAGCTCTCTGAATTCCGTCAGGAACTTCGTCTCCAAATTCGAGTCTTTAAACTTGCCTCTTAATCTCCTCATGTAATTCATCATAAATCTCTCGCCAATTCATCCGTTGAATCGAACAGAGAAACGACATTCGTTAATTGAGAAACTAAAATCATGTATAGTTCTTTTTCAGAAACAACGCAGGCAGATTCTGTTACGAACATGCGATTTCAGAAGACGGAATTGAGATGACGTTCACTACTAATTATCTAGGTCAGTACATCGGAGAACAAATTTTATTTTCAGGTCATTTTCTGTTGACTAAGCTTTTGCTGAATAAGATGATAGAGACGGCCAAATTGACAGGAATCCAAGGCCGAATAGTGAATTTGACGTCCAATATCCACAGCTGGTTTTCCGGCGACATATTCGAGTATCTCGGCCGGATAAGCAGGAGCAACAGGTGTCATTCCGGCCCTGGTAGTAACAGTACTCAATAAAATTTCCTTTGCTCACGTATTAATGGTGGTCAGATCTTCATTCACAGCAGAGAATACGACGCGACACGTGCTTACGCGCTCTCGAAGCTCGCCAATGTCTTGCACACCGTCCAACTTGCTCACAGACTCCAGGTTCCTCCATGGATCTTATTTGGTATTACTTTAGATCAATTAATGAATTCACGAGTTGGTTTTTAATTTGACAAAATCTAAACAGGAAACAGAGGCAAATGTTACGGTCAACTGTGTTCATCCGGGCGTTGTGAGAACCAAATTGAACAGAGATCGTGAAGGCTTCGTCACAGGTCAGTACCTGTGAAACGTACGATTAAGAAATTAATCAATCTGAAACGTACGATTGATTGATTAATAAGAAAATGCAGATTTGATTTTCTACATGGCGTCCAAAACAAAGTTGTTGAAGACGATTCCTCAAGGAGCAGCAACGAGTTGTTACGTAGCCACGAATCGGAACGTTGAGAATGTGAACGGAAAATACTTCGCGGATTGCAACGAACAAGGAAGCTGCGAATCCAAATTAGGTACCAAATCCGCGCCCTTGGCTGCACGCCTATGGTCCGCCTCTGAA

mRNA sequence

ATGCTTGAAATCCTGAAGTACCTCGTCGGTTCAGCCGGAGCAAGTGGCTACGGCTCCAAATCCACCGCCGTAGAGGTCACTCAATCCTGTCCTCATCTGTGCTCCGTCACCGCCATAATCACAGGAGCGACGTCGGGAATCGGAGAGGAGACGGCGAGAGTCTTAGCCAAGCGCGGCGCCAGGATCGTCCTTCCGGCTCGCAACCTCAAAGCCGCCGAGGAAGCAAAGGCGCGCATCAAATCGGAATCGGAATCGGAATGTTCCGATCATTCACGAATCACGGTTATGGAACTCGATCTTAGCTCTCTGAATTCCGTCAGGAACTTCGTCTCCAAATTCGAGTCTTTAAACTTGCCTCTTAATCTCCTCATAAACAACGCAGGCAGATTCTGTTACGAACATGCGATTTCAGAAGACGGAATTGAGATGACGTTCACTACTAATTATCTAGGTCATTTTCTGTTGACTAAGCTTTTGCTGAATAAGATGATAGAGACGGCCAAATTGACAGGAATCCAAGGCCGAATAGTGAATTTGACGTCCAATATCCACAGCTGGTTTTCCGGCGACATATTCGAGTATCTCGGCCGGATAAGCAGGAGCAACAGCAGAGAATACGACGCGACACGTGCTTACGCGCTCTCGAAGCTCGCCAATGTCTTGCACACCGTCCAACTTGCTCACAGACTCCAGGAAACAGAGGCAAATGTTACGGTCAACTGTGTTCATCCGGGCGTTGTGAGAACCAAATTGAACAGAGATCGTGAAGGCTTCGTCACAGATTTGATTTTCTACATGGCGTCCAAAACAAAGTTGTTGAAGACGATTCCTCAAGGAGCAGCAACGAGTTGTTACGTAGCCACGAATCGGAACGTTGAGAATGTGAACGGAAAATACTTCGCGGATTGCAACGAACAAGGAAGCTGCGAATCCAAATTAGGTACCAAATCCGCGCCCTTGGCTGCACGCCTATGGTCCGCCTCTGAA

Coding sequence (CDS)

ATGCTTGAAATCCTGAAGTACCTCGTCGGTTCAGCCGGAGCAAGTGGCTACGGCTCCAAATCCACCGCCGTAGAGGTCACTCAATCCTGTCCTCATCTGTGCTCCGTCACCGCCATAATCACAGGAGCGACGTCGGGAATCGGAGAGGAGACGGCGAGAGTCTTAGCCAAGCGCGGCGCCAGGATCGTCCTTCCGGCTCGCAACCTCAAAGCCGCCGAGGAAGCAAAGGCGCGCATCAAATCGGAATCGGAATCGGAATGTTCCGATCATTCACGAATCACGGTTATGGAACTCGATCTTAGCTCTCTGAATTCCGTCAGGAACTTCGTCTCCAAATTCGAGTCTTTAAACTTGCCTCTTAATCTCCTCATAAACAACGCAGGCAGATTCTGTTACGAACATGCGATTTCAGAAGACGGAATTGAGATGACGTTCACTACTAATTATCTAGGTCATTTTCTGTTGACTAAGCTTTTGCTGAATAAGATGATAGAGACGGCCAAATTGACAGGAATCCAAGGCCGAATAGTGAATTTGACGTCCAATATCCACAGCTGGTTTTCCGGCGACATATTCGAGTATCTCGGCCGGATAAGCAGGAGCAACAGCAGAGAATACGACGCGACACGTGCTTACGCGCTCTCGAAGCTCGCCAATGTCTTGCACACCGTCCAACTTGCTCACAGACTCCAGGAAACAGAGGCAAATGTTACGGTCAACTGTGTTCATCCGGGCGTTGTGAGAACCAAATTGAACAGAGATCGTGAAGGCTTCGTCACAGATTTGATTTTCTACATGGCGTCCAAAACAAAGTTGTTGAAGACGATTCCTCAAGGAGCAGCAACGAGTTGTTACGTAGCCACGAATCGGAACGTTGAGAATGTGAACGGAAAATACTTCGCGGATTGCAACGAACAAGGAAGCTGCGAATCCAAATTAGGTACCAAATCCGCGCCCTTGGCTGCACGCCTATGGTCCGCCTCTGAA

Protein sequence

MLEILKYLVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGARIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPLNLLINNAGRFCYEHAISEDGIEMTFTTNYLGHFLLTKLLLNKMIETAKLTGIQGRIVNLTSNIHSWFSGDIFEYLGRISRSNSREYDATRAYALSKLANVLHTVQLAHRLQETEANVTVNCVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATNRNVENVNGKYFADCNEQGSCESKLGTKSAPLAARLWSASE

Homology

[Close]

BLAST of MC10g1387 vs. ExPASy Swiss-Prot
Match: A0A078IS66 (Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=TIC32A PE=2 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 5.8e-66
Identity = 155/331 (46.83%), Postives = 208/331 (62.84%), Query Frame = 0


Query: 2   LEILKYLVGSAGASGYGSKSTAVEVTQS--CPHLCSVTAIITGATSGIGEETARVLAKRG 61
           +EI   + G AG SGYGS STA +VT S    HL   TAIITG TSGIG E ARVL  RG
Sbjct: 1   MEIYGMVTGKAGKSGYGSASTAEDVTHSIDAKHL---TAIITGGTSGIGLEAARVLGMRG 60

Query: 62  ARIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLP 121
           A +++ +RN KAA ++K  I      +   ++RI  ++LDLSS+ SVR+F+ +F +LN+P
Sbjct: 61  AHVIIASRNTKAANDSKEMI-----LQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVP 120

Query: 122 LNLLINNAGRFCYEHAISEDGIEMTFTTNYLGHFLLTKLLLNKMIETAKLTGIQGRIVNL 181
           LN+LINNAG       +SEDGIE  F TN++GHFLLT LLL+KM  +A+ +GI+GRIVNL
Sbjct: 121 LNILINNAGVMFCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNL 180

Query: 182 TSNIH--SWFSGDIFEYLGRISRSNSREYDATRAYALSKLANVLHTVQLAHRLQETEANV 241
           +S  H  ++  G +F+Y+    R     Y   +AY  SKLAN+LH+  L+ +LQE   N+
Sbjct: 181 SSIAHTYTYTEGIMFDYINDPDR-----YSEKKAYGQSKLANLLHSNALSRKLQEEGVNI 240

Query: 242 TVNCVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATNRNVENVNG 301
           T+N VHPG++ T L R        +    A    L K IPQGAAT+CYVA + ++++V G
Sbjct: 241 TINSVHPGLITTNLFRHSG---LGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKDVTG 300

Query: 302 KYFADCNEQGSCESKLGTKSAPLAARLWSAS 329
           KYFADCN        + T    LA +LW  S
Sbjct: 301 KYFADCNVTTPSNFAIDT---TLADKLWDFS 312

[Close]

BLAST of MC10g1387 vs. ExPASy Swiss-Prot
Match: A0A078ISJ6 (Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=TIC32B PE=3 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 9.9e-66
Identity = 155/331 (46.83%), Postives = 206/331 (62.24%), Query Frame = 0


Query: 2   LEILKYLVGSAGASGYGSKSTAVEVTQS--CPHLCSVTAIITGATSGIGEETARVLAKRG 61
           +EI   + G AG SGYGS STA +VT S    HL   TAIITG TSGIG E ARVL  RG
Sbjct: 1   MEIYGMVTGKAGKSGYGSASTAEDVTHSIDAKHL---TAIITGGTSGIGLEAARVLGMRG 60

Query: 62  ARIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLP 121
           A +++ ARN KAA ++K  I      +   ++RI  ++LDLSS+ SVR+F+ +F +LN+P
Sbjct: 61  AHVIIAARNTKAANDSKEMI-----LQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVP 120

Query: 122 LNLLINNAGRFCYEHAISEDGIEMTFTTNYLGHFLLTKLLLNKMIETAKLTGIQGRIVNL 181
           LN+LINNAG       +SEDGIE  F TN++GHFLLT LLL+KM  +A+ +GI+GRIVNL
Sbjct: 121 LNILINNAGVMFCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNL 180

Query: 182 TSNIH--SWFSGDIFEYLGRISRSNSREYDATRAYALSKLANVLHTVQLAHRLQETEANV 241
           +S  H  ++  G +F+Y+    R     Y   +AY  SKLAN+LH+  L+ +LQE   N+
Sbjct: 181 SSIAHTYTYTEGIMFDYINDPDR-----YSEKKAYGQSKLANLLHSNALSRKLQEEGVNI 240

Query: 242 TVNCVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATNRNVENVNG 301
           T+N VHPG++ T L R        +    A    L K IPQGAAT+CYVA + +++ V G
Sbjct: 241 TINSVHPGLITTNLFRHSG---LGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKGVTG 300

Query: 302 KYFADCNEQGSCESKLGTKSAPLAARLWSAS 329
           KYF DCN        + T    LA +LW  S
Sbjct: 301 KYFTDCNVTTPSNFAIDT---TLADKLWDFS 312

[Close]

BLAST of MC10g1387 vs. ExPASy Swiss-Prot
Match: A2RVM0 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 3.4e-58
Identity = 141/324 (43.52%), Postives = 195/324 (60.19%), Query Frame = 0


Query: 7   YLVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGARIVLPA 66
           +  GS GASG+ S+STA EVT        +TAI+TGA+SGIG ETARVL+ RG  +V+  
Sbjct: 2   WFFGSKGASGFSSRSTAEEVTHGVDG-TGLTAIVTGASSGIGVETARVLSLRGVHVVMAV 61

Query: 67  RNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPLNLLINN 126
           RN  +  + K  I  +        +++ VMELDLSS+ SVR F S+++S  LPLNLLINN
Sbjct: 62  RNTDSGAKVKEDIVKQVPG-----AKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINN 121

Query: 127 AGRFCYEHAISEDGIEMTFTTNYLGHFLLTKLLLNKMIETAKLTGIQGRIVNLTSNIH-- 186
           AG       +S+D IE+ F TN+LGHFLLTKLLL+ M  T++ +  +GRIVNL+S  H  
Sbjct: 122 AGIMACPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRF 181

Query: 187 SWFSGDIFEYLGRISRSNSREYDATRAYALSKLANVLHTVQLAHRLQETEANVTVNCVHP 246
           S+  G  F+ +     ++   Y + RAY  SKL NVLH  +L  +L+E   N+T N +HP
Sbjct: 182 SYPEGVRFDKI-----NDKSSYSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHP 241

Query: 247 GVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATNRNVENVNGKYFADCN 306
           G + T L R    ++   +  +A    +LK++PQGAAT+CYVA N  V  V+G+YF D N
Sbjct: 242 GAIMTNLGRYFNPYLAVAVGAVAK--YILKSVPQGAATTCYVALNPQVAGVSGEYFQDSN 301

Query: 307 EQGSCESKLGTKSAPLAARLWSAS 329
                +     K   LA ++W  S
Sbjct: 302 ---IAKPLPLVKDTELAKKVWDFS 309

[Close]

BLAST of MC10g1387 vs. ExPASy Swiss-Prot
Match: Q6RVV4 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 8.7e-54
Identity = 135/318 (42.45%), Postives = 189/318 (59.43%), Query Frame = 0


Query: 13  GASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGARIVLPARNLKAA 72
           G SG+   STA +VT        +TAI+TGA+SGIG ET RVLA RGA +++  RN+ AA
Sbjct: 9   GVSGFSGSSTAEQVTHGI-DATGLTAIVTGASSGIGAETTRVLALRGAHVIMGVRNMVAA 68

Query: 73  EEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPLNLLINNAGRFCY 132
           ++ K  I  +  S     +++  +ELDLSSL+SV+ F S+F S   PLN+LINNAG    
Sbjct: 69  KDVKDTILKDIPS-----AKVDAIELDLSSLDSVKKFASEFNSSGRPLNILINNAGIMAC 128

Query: 133 EHAISEDGIEMTFTTNYLGHFLLTKLLLNKMIETAKLTGIQGRIVNLTSNIH--SWFSGD 192
              +S+D IE+ F TN++GHFLLT LLL+ M +T + +  +GRIVN+ S  H  ++  G 
Sbjct: 129 PFKLSKDNIELQFATNHIGHFLLTNLLLDTMKKTTRESKKEGRIVNVASEAHRFAYPEGI 188

Query: 193 IFEYLGRISRSNSREYDATRAYALSKLANVLHTVQLAHRLQETEANVTVNCVHPGVVRTK 252
            F+ +     ++   Y+  RAY  SKLANVLH  QL   L+E   N+T N +HPG + T 
Sbjct: 189 RFDKI-----NDQSSYNNWRAYGQSKLANVLHANQLTKHLKEDGVNITANSLHPGTIVTN 248

Query: 253 LNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATNRNVENVNGKYFADCNEQGSCE 312
           L R     V  LI  +     +LK + QGAAT+CYVA +  V+ V+G+YF+D N     +
Sbjct: 249 LFRHNSA-VNGLINVIGK--LVLKNVQQGAATTCYVALHPQVKGVSGEYFSDSNVY---K 308

Query: 313 SKLGTKSAPLAARLWSAS 329
           +    K   LA +LW  S
Sbjct: 309 TTPHGKDVDLAKKLWDFS 309

[Close]

BLAST of MC10g1387 vs. ExPASy Swiss-Prot
Match: Q9HBH5 (Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 1.2e-39
Identity = 121/303 (39.93%), Postives = 171/303 (56.44%), Query Frame = 0


Query: 37  TAIITGATSGIGEETARVLAKRGARIVLPARNLKAAEEAKARIKSE--SESEC------S 96
           T +ITGA SG+G  TA  L + GAR+++  R+   AEEA  +++ E    +EC      S
Sbjct: 45  TVLITGANSGLGRATAAELLRLGARVIMGCRDRARAEEAAGQLRRELRQAAECGPEPGVS 104

Query: 97  DHSRITVMELDLSSLNSVRNFVSKFESLNLPLNLLINNAGRFCYEHAISEDGIEMTFTTN 156
               + V ELDL+SL SVR F  +       L++LINNAG F   +  +EDG EM F  N
Sbjct: 105 GVGELIVRELDLASLRSVRAFCQEMLQEEPRLDVLINNAGIFQCPYMKTEDGFEMQFGVN 164

Query: 157 YLGHFLLTKLLLNKMIETAKLTGIQGRIVNLTSNIHSWFSGDI-FEYLGRISRSNSREYD 216
           +LGHFLLT LLL  +  +A       RIV ++S ++ +  GDI F+ L     ++ + Y+
Sbjct: 165 HLGHFLLTNLLLGLLKSSA-----PSRIVVVSSKLYKY--GDINFDDL-----NSEQSYN 224

Query: 217 ATRAYALSKLANVLHTVQLAHRLQETEANVTVNCVHPGVVRTKLNRD-REGFVTDLIFYM 276
            +  Y+ SKLAN+L T +LA RL+ T  NVTVN +HPG+VRT L R      +   +F +
Sbjct: 225 KSFCYSRSKLANILFTRELARRLEGT--NVTVNVLHPGIVRTNLGRHIHIPLLVKPLFNL 284

Query: 277 ASKTKLLKTIPQGAATSCYVATNRNVENVNGKYFADCNEQGSCESKLGTKSAPLAARLWS 330
            S     KT  +GA TS Y+A++  VE V+G+YF DC E+   E         +A +LW 
Sbjct: 285 VS-WAFFKTPVEGAQTSIYLASSPEVEGVSGRYFGDCKEE---ELLPKAMDESVARKLWD 329

[Close]

BLAST of MC10g1387 vs. NCBI nr
Match: XP_022145402.1 (short-chain dehydrogenase TIC 32, chloroplastic isoform X2 [Momordica charantia])

HSP 1 Score: 633 bits (1633), Expect = 4.38e-228
Identity = 329/340 (96.76%), Postives = 329/340 (96.76%), Query Frame = 0


Query: 1   MLEILKYLVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGA 60
           MLEILKYLVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGA
Sbjct: 1   MLEILKYLVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGA 60

Query: 61  RIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPL 120
           RIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPL
Sbjct: 61  RIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPL 120

Query: 121 NLLINNAGRFCYEHAISEDGIEMTFTTNYLG-----------HFLLTKLLLNKMIETAKL 180
           NLLINNAGRFCYEHAISEDGIEMTFTTNYLG           HFLLTKLLLNKMIETAKL
Sbjct: 121 NLLINNAGRFCYEHAISEDGIEMTFTTNYLGQYIGEQILFSGHFLLTKLLLNKMIETAKL 180

Query: 181 TGIQGRIVNLTSNIHSWFSGDIFEYLGRISRSNSREYDATRAYALSKLANVLHTVQLAHR 240
           TGIQGRIVNLTSNIHSWFSGDIFEYLGRISRSNSREYDATRAYALSKLANVLHTVQLAHR
Sbjct: 181 TGIQGRIVNLTSNIHSWFSGDIFEYLGRISRSNSREYDATRAYALSKLANVLHTVQLAHR 240

Query: 241 LQETEANVTVNCVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATN 300
           LQETEANVTVNCVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATN
Sbjct: 241 LQETEANVTVNCVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATN 300

Query: 301 RNVENVNGKYFADCNEQGSCESKLGTKSAPLAARLWSASE 329
           RNVENVNGKYFADCNEQGSCESKLGTKSAPLAARLWSASE
Sbjct: 301 RNVENVNGKYFADCNEQGSCESKLGTKSAPLAARLWSASE 340

[Close]

BLAST of MC10g1387 vs. NCBI nr
Match: XP_022145401.1 (short-chain dehydrogenase TIC 32, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 627 bits (1617), Expect = 1.45e-225
Identity = 328/340 (96.47%), Postives = 328/340 (96.47%), Query Frame = 0


Query: 1   MLEILKYLVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGA 60
           MLEILKYLVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGA
Sbjct: 1   MLEILKYLVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGA 60

Query: 61  RIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPL 120
           RIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPL
Sbjct: 61  RIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPL 120

Query: 121 NLLINNAGRFCYEHAISEDGIEMTFTTNYLG-----------HFLLTKLLLNKMIETAKL 180
           NLLINNAGRFCYEHAISEDGIEMTFTTNYLG           HFLLTKLLLNKMIETAKL
Sbjct: 121 NLLINNAGRFCYEHAISEDGIEMTFTTNYLGQYIGEQILFSGHFLLTKLLLNKMIETAKL 180

Query: 181 TGIQGRIVNLTSNIHSWFSGDIFEYLGRISRSNSREYDATRAYALSKLANVLHTVQLAHR 240
           TGIQGRIVNLTSNIHSWFSGDIFEYLGRISRSN REYDATRAYALSKLANVLHTVQLAHR
Sbjct: 181 TGIQGRIVNLTSNIHSWFSGDIFEYLGRISRSN-REYDATRAYALSKLANVLHTVQLAHR 240

Query: 241 LQETEANVTVNCVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATN 300
           LQETEANVTVNCVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATN
Sbjct: 241 LQETEANVTVNCVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATN 300

Query: 301 RNVENVNGKYFADCNEQGSCESKLGTKSAPLAARLWSASE 329
           RNVENVNGKYFADCNEQGSCESKLGTKSAPLAARLWSASE
Sbjct: 301 RNVENVNGKYFADCNEQGSCESKLGTKSAPLAARLWSASE 339

[Close]

BLAST of MC10g1387 vs. NCBI nr
Match: XP_022145404.1 (short-chain dehydrogenase TIC 32, chloroplastic isoform X4 [Momordica charantia])

HSP 1 Score: 593 bits (1529), Expect = 1.22e-212
Identity = 309/329 (93.92%), Postives = 309/329 (93.92%), Query Frame = 0


Query: 1   MLEILKYLVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGA 60
           MLEILKYLVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGA
Sbjct: 1   MLEILKYLVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGA 60

Query: 61  RIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPL 120
           RIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPL
Sbjct: 61  RIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPL 120

Query: 121 NLLINNAGRFCYEHAISEDGIEMTFTTNYLGHFLLTKLLLNKMIETAKLTGIQGRIVNLT 180
           NLLINNAGRFCYEHAISEDGIEMTFTTNYLG                    IQGRIVNLT
Sbjct: 121 NLLINNAGRFCYEHAISEDGIEMTFTTNYLG--------------------IQGRIVNLT 180

Query: 181 SNIHSWFSGDIFEYLGRISRSNSREYDATRAYALSKLANVLHTVQLAHRLQETEANVTVN 240
           SNIHSWFSGDIFEYLGRISRSNSREYDATRAYALSKLANVLHTVQLAHRLQETEANVTVN
Sbjct: 181 SNIHSWFSGDIFEYLGRISRSNSREYDATRAYALSKLANVLHTVQLAHRLQETEANVTVN 240

Query: 241 CVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATNRNVENVNGKYF 300
           CVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATNRNVENVNGKYF
Sbjct: 241 CVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATNRNVENVNGKYF 300

Query: 301 ADCNEQGSCESKLGTKSAPLAARLWSASE 329
           ADCNEQGSCESKLGTKSAPLAARLWSASE
Sbjct: 301 ADCNEQGSCESKLGTKSAPLAARLWSASE 309

[Close]

BLAST of MC10g1387 vs. NCBI nr
Match: XP_022145403.1 (short-chain dehydrogenase TIC 32, chloroplastic isoform X3 [Momordica charantia])

HSP 1 Score: 555 bits (1429), Expect = 2.26e-197
Identity = 288/299 (96.32%), Postives = 288/299 (96.32%), Query Frame = 0


Query: 42  GATSGIGEETARVLAKRGARIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLS 101
           GATSGIGEETARVLAKRGARIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLS
Sbjct: 13  GATSGIGEETARVLAKRGARIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLS 72

Query: 102 SLNSVRNFVSKFESLNLPLNLLINNAGRFCYEHAISEDGIEMTFTTNYLG---------- 161
           SLNSVRNFVSKFESLNLPLNLLINNAGRFCYEHAISEDGIEMTFTTNYLG          
Sbjct: 73  SLNSVRNFVSKFESLNLPLNLLINNAGRFCYEHAISEDGIEMTFTTNYLGQYIGEQILFS 132

Query: 162 -HFLLTKLLLNKMIETAKLTGIQGRIVNLTSNIHSWFSGDIFEYLGRISRSNSREYDATR 221
            HFLLTKLLLNKMIETAKLTGIQGRIVNLTSNIHSWFSGDIFEYLGRISRSNSREYDATR
Sbjct: 133 GHFLLTKLLLNKMIETAKLTGIQGRIVNLTSNIHSWFSGDIFEYLGRISRSNSREYDATR 192

Query: 222 AYALSKLANVLHTVQLAHRLQETEANVTVNCVHPGVVRTKLNRDREGFVTDLIFYMASKT 281
           AYALSKLANVLHTVQLAHRLQETEANVTVNCVHPGVVRTKLNRDREGFVTDLIFYMASKT
Sbjct: 193 AYALSKLANVLHTVQLAHRLQETEANVTVNCVHPGVVRTKLNRDREGFVTDLIFYMASKT 252

Query: 282 KLLKTIPQGAATSCYVATNRNVENVNGKYFADCNEQGSCESKLGTKSAPLAARLWSASE 329
           KLLKTIPQGAATSCYVATNRNVENVNGKYFADCNEQGSCESKLGTKSAPLAARLWSASE
Sbjct: 253 KLLKTIPQGAATSCYVATNRNVENVNGKYFADCNEQGSCESKLGTKSAPLAARLWSASE 311

[Close]

BLAST of MC10g1387 vs. NCBI nr
Match: XP_011650304.1 (short-chain dehydrogenase TIC 32 A, chloroplastic isoform X1 [Cucumis sativus] >KAE8650011.1 hypothetical protein Csa_011335 [Cucumis sativus])

HSP 1 Score: 543 bits (1400), Expect = 7.34e-193
Identity = 285/331 (86.10%), Postives = 302/331 (91.24%), Query Frame = 0


Query: 1   MLEILKYLVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGA 60
           MLE+LKYLVGSAGASGYGSKSTA EVTQS  HL S+TAI+TGATSGIGEETARVLAK GA
Sbjct: 1   MLEMLKYLVGSAGASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKGGA 60

Query: 61  RIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPL 120
           RIV+PARNLKAAEEAKARI SE E  C DHSRITVM LDLSSLNSV NFVS+FESLNLPL
Sbjct: 61  RIVIPARNLKAAEEAKARIVSELE--CFDHSRITVMVLDLSSLNSVMNFVSEFESLNLPL 120

Query: 121 NLLINNAGRFCYEHAISEDGIEMTFTTNYLGHFLLTKLLLNKMIETAKLTGIQGRIVNLT 180
           NLLINNAGRFCYEHAISEDGIEMTF TNYLGHFLLTKLLLNKMIETA+ TGIQGRIVN+T
Sbjct: 121 NLLINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAESTGIQGRIVNVT 180

Query: 181 SNIHSWFSGDIFEYLGRISRSNS--REYDATRAYALSKLANVLHTVQLAHRLQETEANVT 240
           SNIHSWFSGD+FEYL +ISR+N+  REYDATRAYA SKLAN+LHTVQLAHRLQE +ANVT
Sbjct: 181 SNIHSWFSGDVFEYLSQISRNNNKYREYDATRAYAFSKLANLLHTVQLAHRLQEMKANVT 240

Query: 241 VNCVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATNRNVENVNGK 300
           VNCVHPGVVRT LNRDREGF+ DLIF+MASK+KLLKTIPQ AATSCYVAT+R VENVNGK
Sbjct: 241 VNCVHPGVVRTNLNRDREGFIKDLIFFMASKSKLLKTIPQSAATSCYVATHRKVENVNGK 300

Query: 301 YFADCNEQGSCESKLGTKSAPLAARLWSASE 329
           YFADCNEQGSCES   TKSA LA RLWS SE
Sbjct: 301 YFADCNEQGSCESN--TKSAHLAERLWSTSE 327

[Close]

BLAST of MC10g1387 vs. ExPASy TrEMBL
Match: A0A6J1CWG8 (short-chain dehydrogenase TIC 32, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111014858 PE=4 SV=1)

HSP 1 Score: 633 bits (1633), Expect = 2.12e-228
Identity = 329/340 (96.76%), Postives = 329/340 (96.76%), Query Frame = 0


Query: 1   MLEILKYLVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGA 60
           MLEILKYLVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGA
Sbjct: 1   MLEILKYLVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGA 60

Query: 61  RIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPL 120
           RIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPL
Sbjct: 61  RIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPL 120

Query: 121 NLLINNAGRFCYEHAISEDGIEMTFTTNYLG-----------HFLLTKLLLNKMIETAKL 180
           NLLINNAGRFCYEHAISEDGIEMTFTTNYLG           HFLLTKLLLNKMIETAKL
Sbjct: 121 NLLINNAGRFCYEHAISEDGIEMTFTTNYLGQYIGEQILFSGHFLLTKLLLNKMIETAKL 180

Query: 181 TGIQGRIVNLTSNIHSWFSGDIFEYLGRISRSNSREYDATRAYALSKLANVLHTVQLAHR 240
           TGIQGRIVNLTSNIHSWFSGDIFEYLGRISRSNSREYDATRAYALSKLANVLHTVQLAHR
Sbjct: 181 TGIQGRIVNLTSNIHSWFSGDIFEYLGRISRSNSREYDATRAYALSKLANVLHTVQLAHR 240

Query: 241 LQETEANVTVNCVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATN 300
           LQETEANVTVNCVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATN
Sbjct: 241 LQETEANVTVNCVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATN 300

Query: 301 RNVENVNGKYFADCNEQGSCESKLGTKSAPLAARLWSASE 329
           RNVENVNGKYFADCNEQGSCESKLGTKSAPLAARLWSASE
Sbjct: 301 RNVENVNGKYFADCNEQGSCESKLGTKSAPLAARLWSASE 340

[Close]

BLAST of MC10g1387 vs. ExPASy TrEMBL
Match: A0A6J1CW75 (short-chain dehydrogenase TIC 32, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111014858 PE=4 SV=1)

HSP 1 Score: 627 bits (1617), Expect = 7.00e-226
Identity = 328/340 (96.47%), Postives = 328/340 (96.47%), Query Frame = 0


Query: 1   MLEILKYLVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGA 60
           MLEILKYLVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGA
Sbjct: 1   MLEILKYLVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGA 60

Query: 61  RIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPL 120
           RIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPL
Sbjct: 61  RIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPL 120

Query: 121 NLLINNAGRFCYEHAISEDGIEMTFTTNYLG-----------HFLLTKLLLNKMIETAKL 180
           NLLINNAGRFCYEHAISEDGIEMTFTTNYLG           HFLLTKLLLNKMIETAKL
Sbjct: 121 NLLINNAGRFCYEHAISEDGIEMTFTTNYLGQYIGEQILFSGHFLLTKLLLNKMIETAKL 180

Query: 181 TGIQGRIVNLTSNIHSWFSGDIFEYLGRISRSNSREYDATRAYALSKLANVLHTVQLAHR 240
           TGIQGRIVNLTSNIHSWFSGDIFEYLGRISRSN REYDATRAYALSKLANVLHTVQLAHR
Sbjct: 181 TGIQGRIVNLTSNIHSWFSGDIFEYLGRISRSN-REYDATRAYALSKLANVLHTVQLAHR 240

Query: 241 LQETEANVTVNCVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATN 300
           LQETEANVTVNCVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATN
Sbjct: 241 LQETEANVTVNCVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATN 300

Query: 301 RNVENVNGKYFADCNEQGSCESKLGTKSAPLAARLWSASE 329
           RNVENVNGKYFADCNEQGSCESKLGTKSAPLAARLWSASE
Sbjct: 301 RNVENVNGKYFADCNEQGSCESKLGTKSAPLAARLWSASE 339

[Close]

BLAST of MC10g1387 vs. ExPASy TrEMBL
Match: A0A6J1CV51 (short-chain dehydrogenase TIC 32, chloroplastic isoform X4 OS=Momordica charantia OX=3673 GN=LOC111014858 PE=4 SV=1)

HSP 1 Score: 593 bits (1529), Expect = 5.91e-213
Identity = 309/329 (93.92%), Postives = 309/329 (93.92%), Query Frame = 0


Query: 1   MLEILKYLVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGA 60
           MLEILKYLVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGA
Sbjct: 1   MLEILKYLVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGA 60

Query: 61  RIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPL 120
           RIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPL
Sbjct: 61  RIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPL 120

Query: 121 NLLINNAGRFCYEHAISEDGIEMTFTTNYLGHFLLTKLLLNKMIETAKLTGIQGRIVNLT 180
           NLLINNAGRFCYEHAISEDGIEMTFTTNYLG                    IQGRIVNLT
Sbjct: 121 NLLINNAGRFCYEHAISEDGIEMTFTTNYLG--------------------IQGRIVNLT 180

Query: 181 SNIHSWFSGDIFEYLGRISRSNSREYDATRAYALSKLANVLHTVQLAHRLQETEANVTVN 240
           SNIHSWFSGDIFEYLGRISRSNSREYDATRAYALSKLANVLHTVQLAHRLQETEANVTVN
Sbjct: 181 SNIHSWFSGDIFEYLGRISRSNSREYDATRAYALSKLANVLHTVQLAHRLQETEANVTVN 240

Query: 241 CVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATNRNVENVNGKYF 300
           CVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATNRNVENVNGKYF
Sbjct: 241 CVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATNRNVENVNGKYF 300

Query: 301 ADCNEQGSCESKLGTKSAPLAARLWSASE 329
           ADCNEQGSCESKLGTKSAPLAARLWSASE
Sbjct: 301 ADCNEQGSCESKLGTKSAPLAARLWSASE 309

[Close]

BLAST of MC10g1387 vs. ExPASy TrEMBL
Match: A0A6J1CVU9 (short-chain dehydrogenase TIC 32, chloroplastic isoform X3 OS=Momordica charantia OX=3673 GN=LOC111014858 PE=4 SV=1)

HSP 1 Score: 555 bits (1429), Expect = 1.09e-197
Identity = 288/299 (96.32%), Postives = 288/299 (96.32%), Query Frame = 0


Query: 42  GATSGIGEETARVLAKRGARIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLS 101
           GATSGIGEETARVLAKRGARIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLS
Sbjct: 13  GATSGIGEETARVLAKRGARIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLS 72

Query: 102 SLNSVRNFVSKFESLNLPLNLLINNAGRFCYEHAISEDGIEMTFTTNYLG---------- 161
           SLNSVRNFVSKFESLNLPLNLLINNAGRFCYEHAISEDGIEMTFTTNYLG          
Sbjct: 73  SLNSVRNFVSKFESLNLPLNLLINNAGRFCYEHAISEDGIEMTFTTNYLGQYIGEQILFS 132

Query: 162 -HFLLTKLLLNKMIETAKLTGIQGRIVNLTSNIHSWFSGDIFEYLGRISRSNSREYDATR 221
            HFLLTKLLLNKMIETAKLTGIQGRIVNLTSNIHSWFSGDIFEYLGRISRSNSREYDATR
Sbjct: 133 GHFLLTKLLLNKMIETAKLTGIQGRIVNLTSNIHSWFSGDIFEYLGRISRSNSREYDATR 192

Query: 222 AYALSKLANVLHTVQLAHRLQETEANVTVNCVHPGVVRTKLNRDREGFVTDLIFYMASKT 281
           AYALSKLANVLHTVQLAHRLQETEANVTVNCVHPGVVRTKLNRDREGFVTDLIFYMASKT
Sbjct: 193 AYALSKLANVLHTVQLAHRLQETEANVTVNCVHPGVVRTKLNRDREGFVTDLIFYMASKT 252

Query: 282 KLLKTIPQGAATSCYVATNRNVENVNGKYFADCNEQGSCESKLGTKSAPLAARLWSASE 329
           KLLKTIPQGAATSCYVATNRNVENVNGKYFADCNEQGSCESKLGTKSAPLAARLWSASE
Sbjct: 253 KLLKTIPQGAATSCYVATNRNVENVNGKYFADCNEQGSCESKLGTKSAPLAARLWSASE 311

[Close]

BLAST of MC10g1387 vs. ExPASy TrEMBL
Match: A0A5A7UCR1 (Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold344G00880 PE=4 SV=1)

HSP 1 Score: 539 bits (1388), Expect = 2.57e-191
Identity = 284/333 (85.29%), Postives = 301/333 (90.39%), Query Frame = 0


Query: 1   MLEILKYLVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGA 60
           MLE+LKYLVGSAGASGYGS STA EVTQS  HL S+TAI+TGATSGIGEETARVLAKRGA
Sbjct: 1   MLEMLKYLVGSAGASGYGSNSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60

Query: 61  RIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPL 120
           RIV+PARNLKAAEEAKARI SE E  C D+SRITVM LDLSSLNSV NFVS+FE+LNLPL
Sbjct: 61  RIVIPARNLKAAEEAKARIVSELE--CFDYSRITVMVLDLSSLNSVMNFVSEFEALNLPL 120

Query: 121 NLLINNAGRFCYEHAISEDGIEMTFTTNYLGHFLLTKLLLNKMIETAKLTGIQGRIVNLT 180
           NLLINNAGRFCYEHA SEDGIEMTF TNYLGHFLLTKLLLNKMIETAK TGIQGRIVN+T
Sbjct: 121 NLLINNAGRFCYEHATSEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVT 180

Query: 181 SNIHSWFSGDIFEYLGRISRS----NSREYDATRAYALSKLANVLHTVQLAHRLQETEAN 240
           SNIHSWFSGDIFEYL +ISR+    NSREYDATRAY+ SKLAN+LHTVQLAHRLQE +AN
Sbjct: 181 SNIHSWFSGDIFEYLSQISRNYNNNNSREYDATRAYSFSKLANLLHTVQLAHRLQEMKAN 240

Query: 241 VTVNCVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATNRNVENVN 300
           VTVNCVHPGVVRT LNRDREGF+ DLIF+MASK+KLLKTIPQ AATSCYVAT+R VENVN
Sbjct: 241 VTVNCVHPGVVRTNLNRDREGFLKDLIFFMASKSKLLKTIPQSAATSCYVATHRKVENVN 300

Query: 301 GKYFADCNEQGSCESKLGTKSAPLAARLWSASE 329
           GKYF DCNEQGSCES   TKSA LA RLWSASE
Sbjct: 301 GKYFEDCNEQGSCESN--TKSAHLAERLWSASE 329

[Close]

BLAST of MC10g1387 vs. TAIR 10
Match: AT4G24050.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 421.0 bits (1081), Expect = 8.7e-118
Identity = 228/329 (69.30%), Postives = 271/329 (82.37%), Query Frame = 0


Query: 1   MLEILKYLVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGA 60
           M+E  KYL+G+AGASG+GSKSTA EVT++C  L S+TA+ITGATSGIG ETARVLAKRGA
Sbjct: 1   MIETGKYLLGAAGASGFGSKSTAEEVTENC-DLRSITAVITGATSGIGAETARVLAKRGA 60

Query: 61  RIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPL 120
           R++ PARN+KAAEEAK RI SE        + I VM+LDLSS+ SVRNFV+ FESL+LPL
Sbjct: 61  RLIFPARNVKAAEEAKERIVSE-----FPETEIVVMKLDLSSIASVRNFVADFESLDLPL 120

Query: 121 NLLINNAGRFCYEHAISEDGIEMTFTTNYLGHFLLTKLLLNKMIETAKLTGIQGRIVNLT 180
           NLLINNAG+  +EHAISEDGIEMTF TNYLGHFLLT LLLNKMI+TA+ TG+QGRIVN+T
Sbjct: 121 NLLINNAGKLAHEHAISEDGIEMTFATNYLGHFLLTNLLLNKMIQTAEETGVQGRIVNVT 180

Query: 181 SNIHSWFSGDIFEYLGRISRSNSREYDATRAYALSKLANVLHTVQLAHRLQETEANVTVN 240
           S IH WFSGD+ EYL  IS+    ++DATRAYALSKLANVLHT +L+ RLQ+  ANVTVN
Sbjct: 181 SGIHGWFSGDLIEYLRLISQPKC-QFDATRAYALSKLANVLHTKELSSRLQKIGANVTVN 240

Query: 241 CVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATNRNVENVNGKYF 300
           CVHPGVVRT+L RDREG +TDL+F++AS  KL+KT+PQ AAT+CYVATN  + NV+GKYF
Sbjct: 241 CVHPGVVRTRLTRDREGLLTDLVFFLAS--KLVKTVPQAAATTCYVATNPRLVNVSGKYF 300

Query: 301 ADCNEQGSCESKLGTKSAPLAARLWSASE 330
            DCNE  +  S LGT S+  A +LW+ASE
Sbjct: 301 TDCNE--TTPSGLGTNSSE-ATKLWAASE 317

[Close]

BLAST of MC10g1387 vs. TAIR 10
Match: AT1G64590.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 403.3 bits (1035), Expect = 1.9e-112
Identity = 219/334 (65.57%), Postives = 262/334 (78.44%), Query Frame = 0


Query: 1   MLEILKYLVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGA 60
           M+E +K+L+GS G SG+GS+STA  VT +   L S+TAIITGATSGIG ETARVLAKRGA
Sbjct: 1   MIETVKHLIGSGGPSGFGSRSTADHVTCN-SDLRSLTAIITGATSGIGAETARVLAKRGA 60

Query: 61  RIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPL 120
           R+VLPAR++K AEE KARI SE        + I VM LDLSSL SVR FV  FESLNLPL
Sbjct: 61  RLVLPARSVKTAEETKARILSEFPD-----AEIIVMHLDLSSLTSVRRFVDDFESLNLPL 120

Query: 121 NLLINNAGRFCYEHAISEDGIEMTFTTNYLGHFLLTKLLLNKMIETAKLTGIQGRIVNLT 180
           N+LINNAG++ ++HA+SEDG+EMTF TNYLGHFLLTKLLL KMIETA  TG+QGRIVN+T
Sbjct: 121 NILINNAGKYAHKHALSEDGVEMTFATNYLGHFLLTKLLLKKMIETAAQTGVQGRIVNVT 180

Query: 181 SNIHSWFSGDIFEYLGRISRSNSREYDATRAYALSKLANVLHTVQLAHRLQETEANVTVN 240
           S +HSWFSGD+ +YL  ISR N+R YDATRAYALSKLANVLHTV+L+  L + +ANVT N
Sbjct: 181 SVVHSWFSGDMLQYLADISR-NNRNYDATRAYALSKLANVLHTVELSRLLHKMDANVTAN 240

Query: 241 CVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATNRNVENVNGKYF 300
           CVHPG+V+T+L RDREG VTDL+F++ S  KLLK++PQ AAT+CYVAT+  + NV GKYF
Sbjct: 241 CVHPGIVKTRLTRDREGVVTDLVFFLTS--KLLKSVPQAAATTCYVATSPRLRNVCGKYF 300

Query: 301 ADCNE-----QGSCESKLGTKSAPLAARLWSASE 330
           +DCNE      GSC  K        A RLW+AS+
Sbjct: 301 SDCNEARSSKSGSCNLK--------AQRLWTASD 317

[Close]

BLAST of MC10g1387 vs. TAIR 10
Match: AT5G50130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 290.4 bits (742), Expect = 1.8e-78
Identity = 164/305 (53.77%), Postives = 216/305 (70.82%), Query Frame = 0


Query: 5   LKYLVGSAGASGYGSKSTAVEVTQSCPHLCS-VTAIITGATSGIGEETARVLAKRGARIV 64
           L+YL G AG +G+GS+STA +VTQ     CS +TAIITG TSGIG ETARVLAKRG R+V
Sbjct: 5   LRYLAGIAGPNGFGSRSTAEQVTQHSFFPCSHLTAIITGGTSGIGAETARVLAKRGVRVV 64

Query: 65  LPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPLNLL 124
           +  R++K AE  K RI  E     +  + I + E+DLSSL+SV  F S+F S +LPLN+L
Sbjct: 65  MAVRDMKKAEMVKERIIRE-----NPEADIILFEIDLSSLSSVARFCSQFLSQDLPLNIL 124

Query: 125 INNAGRFCYEHAISEDGIEMTFTTNYLGHFLLTKLLLNKMIETAKLTGIQGRIVNLTSNI 184
           INNAG F      SE+ IE+TF TN+LGH+LLT++L+ KMI+TA+ +GI+GRI+NL+S I
Sbjct: 125 INNAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVI 184

Query: 185 HSWFSGDIFEY---LGRISRSNSREYDATRAYALSKLANVLHTVQLAHRLQETEANVTVN 244
           H+W   D F +   L  ISR     Y+ TRAYA SKLA +LH   L+ +L++  ANVT+N
Sbjct: 185 HNWVKPDCFSFPKLLHPISR-----YNGTRAYAQSKLATILHAKALSKQLKDRNANVTIN 244

Query: 245 CVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATNRNVENVNGKYF 304
            VHPG+V+T + R  +G  TD +F +AS  KLLK+I QGAAT+CYVA +   + ++GKYF
Sbjct: 245 AVHPGIVKTGIIRAHKGLFTDSLFLIAS--KLLKSISQGAATTCYVALSNETKGLSGKYF 297

Query: 305 ADCNE 306
           ADCNE
Sbjct: 305 ADCNE 297

[Close]

BLAST of MC10g1387 vs. TAIR 10
Match: AT5G02540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 259.2 bits (661), Expect = 4.4e-69
Identity = 156/323 (48.30%), Postives = 212/323 (65.63%), Query Frame = 0


Query: 8   LVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGARIVLPAR 67
           + G  G SG+GS STA EVTQ      ++TAIITG T GIG ETARVL+KRGA +V+ AR
Sbjct: 7   ITGRRGPSGFGSASTAEEVTQGI-DATNLTAIITGGTGGIGMETARVLSKRGAHVVIGAR 66

Query: 68  NLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPLNLLINNA 127
           N+ AAE AK  I  ++      ++R+T+++LDLSS+ S++ FV +F +L+LPLNLLINNA
Sbjct: 67  NMGAAENAKTEILRQNA-----NARVTLLQLDLSSIKSIKAFVREFHALHLPLNLLINNA 126

Query: 128 GRFCYEHAISEDGIEMTFTTNYLGHFLLTKLLLNKMIETAKLTGIQGRIVNLTS--NIHS 187
           G     + +SEDGIE+ F TN++GHFLLT LLL+ M  TAK +G++GRI+N++S  +I++
Sbjct: 127 GVMFCPYQLSEDGIELQFATNHIGHFLLTNLLLDTMKNTAKTSGVEGRILNVSSVAHIYT 186

Query: 188 WFSGDIFEYLGRISRSNSREYDATRAYALSKLANVLHTVQLAHRLQETEANVTVNCVHPG 247
           +  G  F+ +  I       Y   RAY  SKLAN+LH  +L+ +LQE   N+T N VHPG
Sbjct: 187 YQEGIQFDSINDIC-----SYSDKRAYGQSKLANILHANELSRQLQEEGVNITANSVHPG 246

Query: 248 VVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATNRNVENVNGKYFADCNE 307
           ++ T L +     +  L F+      L K IPQGAAT+CYVA + +V+ V GKYFADCNE
Sbjct: 247 LILTNLFQHTALLMRFLKFF---SFYLWKNIPQGAATTCYVALHPSVKGVTGKYFADCNE 306

Query: 308 QGSCESKLGTKSAPLAARLWSAS 329
                SKL  +   LA +LW  S
Sbjct: 307 --VTPSKL-ARDETLAQKLWDFS 312

[Close]

BLAST of MC10g1387 vs. TAIR 10
Match: AT5G50130.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 252.3 bits (643), Expect = 5.4e-67
Identity = 147/278 (52.88%), Postives = 194/278 (69.78%), Query Frame = 0


Query: 5   LKYLVGSAGASGYGSKSTAVEVTQSCPHLCS-VTAIITGATSGIGEETARVLAKRGARIV 64
           L+YL G AG +G+GS+STA +VTQ     CS +TAIITG TSGIG ETARVLAKRG R+V
Sbjct: 5   LRYLAGIAGPNGFGSRSTAEQVTQHSFFPCSHLTAIITGGTSGIGAETARVLAKRGVRVV 64

Query: 65  LPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPLNLL 124
           +  R++K AE  K RI  E     +  + I + E+DLSSL+SV  F S+F S +LPLN+L
Sbjct: 65  MAVRDMKKAEMVKERIIRE-----NPEADIILFEIDLSSLSSVARFCSQFLSQDLPLNIL 124

Query: 125 INNAGRFCYEHAISEDGIEMTFTTNYLGHFLLTKLLLNKMIETAKLTGIQGRIVNLTSNI 184
           INNAG F      SE+ IE+TF TN+LGH+LLT++L+ KMI+TA+ +GI+GRI+NL+S I
Sbjct: 125 INNAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVI 184

Query: 185 HSWFSGDIFEY---LGRISRSNSREYDATRAYALSKLANVLHTVQLAHRLQETEANVTVN 244
           H+W   D F +   L  ISR     Y+ TRAYA SKLA +LH   L+ +L++  ANVT+N
Sbjct: 185 HNWVKPDCFSFPKLLHPISR-----YNGTRAYAQSKLATILHAKALSKQLKDRNANVTIN 244

Query: 245 CVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQ 279
            VHPG+V+T + R  +G  TD +F +AS  KLLK+I Q
Sbjct: 245 AVHPGIVKTGIIRAHKGLFTDSLFLIAS--KLLKSISQ 270

The following BLAST results are available for this feature:

BLAST of MC10g1387 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A078IS66	5.8e-66	46.83	Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A0A078ISJ6	9.9e-66	46.83	Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A2RVM0	3.4e-58	43.52	Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
Q6RVV4	8.7e-54	42.45	Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3...	[more]
Q9HBH5	1.2e-39	39.93	Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1	[more]

BLAST of MC10g1387 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145402.1	4.38e-228	96.76	short-chain dehydrogenase TIC 32, chloroplastic isoform X2 [Momordica charantia]	[more]
XP_022145401.1	1.45e-225	96.47	short-chain dehydrogenase TIC 32, chloroplastic isoform X1 [Momordica charantia]	[more]
XP_022145404.1	1.22e-212	93.92	short-chain dehydrogenase TIC 32, chloroplastic isoform X4 [Momordica charantia]	[more]
XP_022145403.1	2.26e-197	96.32	short-chain dehydrogenase TIC 32, chloroplastic isoform X3 [Momordica charantia]	[more]
XP_011650304.1	7.34e-193	86.10	short-chain dehydrogenase TIC 32 A, chloroplastic isoform X1 [Cucumis sativus] >...	[more]

BLAST of MC10g1387 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CWG8	2.12e-228	96.76	short-chain dehydrogenase TIC 32, chloroplastic isoform X2 OS=Momordica charanti...	[more]
A0A6J1CW75	7.00e-226	96.47	short-chain dehydrogenase TIC 32, chloroplastic isoform X1 OS=Momordica charanti...	[more]
A0A6J1CV51	5.91e-213	93.92	short-chain dehydrogenase TIC 32, chloroplastic isoform X4 OS=Momordica charanti...	[more]
A0A6J1CVU9	1.09e-197	96.32	short-chain dehydrogenase TIC 32, chloroplastic isoform X3 OS=Momordica charanti...	[more]
A0A5A7UCR1	2.57e-191	85.29	Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]

BLAST of MC10g1387 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G24050.1	8.7e-118	69.30	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G64590.1	1.9e-112	65.57	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G50130.1	1.8e-78	53.77	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G02540.1	4.4e-69	48.30	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G50130.2	5.4e-67	52.88	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 37..54 score: 44.95 coord: 118..129 score: 42.55 coord: 168..184 score: 28.04 coord: 212..231 score: 30.28 coord: 235..252 score: 36.86
IPR002347	Short-chain dehydrogenase/reductase SDR	PFAM	PF00106	adh_short	coord: 37..182 e-value: 1.6E-25 score: 89.7
None	No IPR available	GENE3D	3.40.50.720		coord: 24..329 e-value: 4.8E-70 score: 237.9
None	No IPR available	PANTHER	PTHR24320:SF210	MITOCHONDRIAL PYRUVATE CARRIER	coord: 4..329
None	No IPR available	PANTHER	PTHR24320	RETINOL DEHYDROGENASE	coord: 4..329
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 37..304

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g1387.1	MC10g1387.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity