MC10g1385 (gene) Bitter gourd (Dali-11) v1

BLAST of MC10g1385 vs. ExPASy Swiss-Prot
Match: Q9F234 (Alpha-glucosidase 2 OS=Bacillus thermoamyloliquefaciens OX=1425 PE=3 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 4.6e-130
Identity = 238/607 (39.21%), Postives = 350/607 (57.66%), Query Frame = 0

Query: 105 FYGTGEVSGQLERTGKRIFTWNTDAYG-YGAGTTSLYQAHPWVLAILPNGQALGVLADTS 164
           FYG GE +G L++ G+ +  WNTD Y  +   T  LYQ+HP+ + +  NG A G+  D +
Sbjct: 149 FYGFGEKTGFLDKRGETMTMWNTDVYAPHNPETDPLYQSHPYFMTV-RNGSAHGIFFDNT 208

Query: 165 LRCEIDLRKDSIIQFVAPSSYPVITFGPFSSPTA--VLKAFSSAVGTVFMPPKWALGYHQ 224
            +   D  + +  ++   +    I +  F+ PT   VL+ ++   G + +PPKWALGYHQ
Sbjct: 209 YKTTFDF-QTATDEYCFSAEGGAIDYYVFAGPTPKDVLEQYTDLTGRMPLPPKWALGYHQ 268

Query: 225 CRWSYDSADRVLEVSRTFREKDIPCDVIWMDIDYMDGFRCFTFDPERFADPKTLVNELHQ 284
            R+SY++   V E+++TF EKDIP DVI++DI YM+G+R FTFD  RF + K L+ +L Q
Sbjct: 269 SRYSYETEQEVREIAQTFIEKDIPLDVIYLDIHYMNGYRVFTFDRNRFPNLKQLIADLKQ 328

Query: 285 IGFKAIWMLDPGIKHEKGYFVYDSGSEKDIWVQKADGDHYVGEVWPGPCVFPDFTQAKAR 344
            G + + ++DPG+K +  Y +Y  G   D + +  +G+ Y GEVWPG   FPDFT  K R
Sbjct: 329 KGIRVVPIVDPGVKEDPEYVIYQEGIRHDYFCKYIEGNVYFGEVWPGKSAFPDFTNKKVR 388

Query: 345 SWWANLVKDFVSNGVDGIWNDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVY 404
            WW    + +   G++GIWNDMNEP++F   TKTM    IH  D   G  + H   HNVY
Sbjct: 389 KWWGEKHQFYTDLGIEGIWNDMNEPSVFNE-TKTMDVKVIHDND---GDPKTHRELHNVY 448

Query: 405 GMLMAKSTYEGMKLAKSDRRPFVLTRAGFIGSQRYAATWTGDNLSSWDHLHMSISMVLQL 464
           G +M ++TY+GMK   + +RPF+LTRAGF G QRYAA WTGDN S W+HL MS+ M + L
Sbjct: 449 GFMMGEATYKGMKKLLNGKRPFLLTRAGFSGIQRYAAVWTGDNRSFWEHLQMSLPMCMNL 508

Query: 465 GLSGQPLSGPDIGGFAGNATPKLFGRWMGIGALFPFCRGHSETSTADHEPWSFGEECEEV 524
           GLSG    GPD+GGFA N   +L  RWM +GA  P+ R H        EPW+FGE+ E +
Sbjct: 509 GLSGVAFCGPDVGGFAHNTNGELLTRWMQVGAFTPYFRNHCAIGFRRQEPWAFGEKYERI 568

Query: 525 CRLALKRRYRLLPHIYTLFYMAHTTGVPVATPVFFADSRDPKLRKIENSFLLGSILIYSS 584
            +  ++ RY+ LPH+YTLF  AH TG PV  P+FF    D     + + FL+G+ ++ + 
Sbjct: 569 IKKYIRLRYQWLPHLYTLFAEAHETGAPVMRPLFFEYPDDENTYNLYDEFLVGANVLIAP 628

Query: 585 TLPNQGMHNLNFTLPKGVWLRF---------DFDDSHPDLPAL--FLQGGSIVPLGPPHQ 644
            +       + +  PKG W+ +          +     DL  L  F++ GS + LG   +
Sbjct: 629 IMTPSTTRRVAY-FPKGNWVDYWTGEVLEGGQYHLISADLETLPIFIKQGSAIALGDVKR 688

Query: 645 HTGEANPSDDISLLVALDENGKAKGVLFEDDGDGYGFSLGAYLLTHYVAELESSVVTVQV 698
            T    P +  ++ +     GKA  VL++DDG  + +  G YL  +   E   + V + V
Sbjct: 689 ST--EMPDEHRTVHIYKANGGKATYVLYDDDGQTFSYEKGDYLRMYIEVEYGENSVHI-V 745

BLAST of MC10g1385 vs. ExPASy Swiss-Prot
Match: Q4R4N7 (Neutral alpha-glucosidase AB OS=Macaca fascicularis OX=9541 GN=GANAB PE=2 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 3.5e-98
Identity = 192/501 (38.32%), Postives = 271/501 (54.09%), Query Frame = 0

Query: 187 ITFGPFSSPTAVLKAFSSAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFREKDIPC 246
           +  GP  S + V + ++S  GT  +PP ++LGYHQ RW+Y     VLEV + F E ++PC
Sbjct: 366 LLLGP--SISDVFRQYASLTGTQALPPLFSLGYHQSRWNYRDEADVLEVDQGFDEHNLPC 425

Query: 247 DVIWMDIDYMDGFRCFTFDPERFADPKTLVNELHQIGFKAIWMLDPGIKHEKGYFVYDSG 306
           DVIW+DI++ DG R FT+DP RF  P+T++  L     K + ++DP IK + GY V+D  
Sbjct: 426 DVIWLDIEHADGKRYFTWDPSRFPQPRTMLERLASKRRKLVAIVDPHIKVDSGYRVHDEL 485

Query: 307 SEKDIWVQKADGDHYVGEVWPGPCVFPDFTQAKARSWWANLVKDFVSNGVDG------IW 366
               ++V+  DG  Y G  WPG   +PDFT    R+WWAN+   F  +  +G      +W
Sbjct: 486 RNLGLYVKTRDGSDYEGWCWPGSAGYPDFTNPTMRAWWANM---FSYDNYEGSAPNLFVW 545

Query: 367 NDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMAKSTYEGMKLAKSD- 426
           NDMNEP++F       PE  + +  + +GG + H   HN+YG+ +  +T +G++      
Sbjct: 546 NDMNEPSVFNG-----PEVTMLKDAQHYGGWE-HRDVHNIYGLYVHMATADGLRQRSGGM 605

Query: 427 RRPFVLTRAGFIGSQRYAATWTGDNLSSWDHLHMSISMVLQLGLSGQPLSGPDIGGFAGN 486
            RPFVL RA F GSQR+ A WTGDN + WDHL +SI M L LGL G    G D+GGF  N
Sbjct: 606 ERPFVLARAFFAGSQRFGAVWTGDNTAEWDHLKISIPMCLSLGLVGLSFCGADVGGFFKN 665

Query: 487 ATPKLFGRWMGIGALFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTL 546
             P+L  RW  +GA  PF R H+   T    PW    +  ++ R AL +RY LLP  YTL
Sbjct: 666 PEPELLVRWYQMGAYQPFFRAHAHLDTGRRGPWLLPSQHNDIIRDALGQRYSLLPFWYTL 725

Query: 547 FYMAHTTGVPVATPVFFADSRDPKLRKIENSFLLGSILIYSSTLPNQGMHNLNFTLP--K 606
           FY AH  G+P+  P++    +D     I++ +LLG  L+    + + G H +   LP   
Sbjct: 726 FYQAHREGIPIMRPLWVQYPQDVTTFSIDDQYLLGDALLV-HPVSDSGAHGVQVYLPGQG 785

Query: 607 GVWLRFDFDDSHPDLPALFL-----------QGGSIVPLGPPHQHTGEANPSDDISLLVA 666
            VW        H     L+L           +GG+IVP     + + E    D I+L VA
Sbjct: 786 EVWYDIQSYQKHHGPQTLYLPVTLSSIPVFQRGGTIVPRWMRVRRSSECMKDDPITLFVA 845

Query: 667 LDENGKAKGVLFEDDGDGYGF 668
           L   G A+G LF DDG  + +
Sbjct: 846 LSPQGTAEGELFLDDGHTFNY 854

BLAST of MC10g1385 vs. ExPASy Swiss-Prot
Match: B9F676 (Probable glucan 1,3-alpha-glucosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0216600 PE=3 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 6.0e-98
Identity = 191/506 (37.75%), Postives = 270/506 (53.36%), Query Frame = 0

Query: 193 SSPTAVLKAFSSAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFREKDIPCDVIWMD 252
           S P  V+K + S  GT  MP ++A+ YHQCRW+Y   + V  V   F E DIP DV+W+D
Sbjct: 338 SEPKDVIKQYISVTGTPSMPQQFAVAYHQCRWNYRDEEDVAGVDSGFDEHDIPYDVLWLD 397

Query: 253 IDYMDGFRCFTFDPERFADPKTLVNELHQIGFKAIWMLDPGIKHEKGYFVYDSGSEKDIW 312
           I++ DG R FT+D   F +P+ +  ++   G K + ++DP IK +  + +++  + K  +
Sbjct: 398 IEHTDGKRYFTWDHSAFPNPEVMQGKIADKGRKMVTIVDPHIKRDSSFHLHEEATAKGYY 457

Query: 313 VQKADGDHYVGEVWPGPCVFPDFTQAKARSWWANLVKDFVSNGVDG------IWNDMNEP 372
           V+ A G  + G  WPG   +PD    + R WWA+    F      G      IWNDMNEP
Sbjct: 458 VKDATGKDFDGWCWPGASSYPDMLNPEIREWWAD---KFSYENYKGSTPTLYIWNDMNEP 517

Query: 373 AIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMAKSTYEG-MKLAKSDRRPFVL 432
           ++F     TMP   +H GD E      H   HN YG     +T +G +K  +   RPFVL
Sbjct: 518 SVFNGPEVTMPRDAVHYGDVE------HRELHNAYGYYFHMATADGLLKRGEGKDRPFVL 577

Query: 433 TRAGFIGSQRYAATWTGDNLSSWDHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPKLF 492
           +RA F GSQRY A WTGDN + WDHL  SI MVL LGL+G   SG DIGGF GN  P L 
Sbjct: 578 SRAFFAGSQRYGAIWTGDNSADWDHLKSSIPMVLTLGLTGMTFSGADIGGFFGNPEPDLL 637

Query: 493 GRWMGIGALFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLFYMAHT 552
            RW  +GA +PF RGH+   T   EPW FGE    + R A+  RY LLP+ YTLF  A  
Sbjct: 638 VRWYQVGAFYPFFRGHAHHDTKRREPWLFGERRTALMREAIHMRYSLLPYYYTLFREASV 697

Query: 553 TGVPVATPVFFADSRDPKLRKIENSFLLGSILIYSSTLPNQGMHNLNFTLPKGVWLRFDF 612
           TGVPV  P++     D +      +F++G  L+ +  +  +G  +++  LP G  L +D 
Sbjct: 698 TGVPVMRPLWLEFPDDKETYNNGEAFMVGPSLL-AQGIYEEGQKSVSVYLP-GEELWYDL 757

Query: 613 DDSHP-------------DLPALFLQGGSIVPLGPPHQHTGEANPSDDISLLVALDENGK 672
            +  P             D    F + G+IVP     + +     +D  +L++AL+ +  
Sbjct: 758 RNGSPYKGGVSHKLEVSEDSIPSFQRAGAIVPRKDRFRRSSTQMVNDPYTLVIALNSSSA 817

Query: 673 AKGVLFEDDGDGYGFSLGAYLLTHYV 679
           A+G L+ DDG  Y +  GA++   +V
Sbjct: 818 AEGELYVDDGKSYDYQQGAFIHRRFV 832

BLAST of MC10g1385 vs. ExPASy Swiss-Prot
Match: Q8BHN3 (Neutral alpha-glucosidase AB OS=Mus musculus OX=10090 GN=Ganab PE=1 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 1.0e-97
Identity = 196/531 (36.91%), Postives = 277/531 (52.17%), Query Frame = 0

Query: 198 VLKAFSSAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFREKDIPCDVIWMDIDYMD 257
           V + ++S  GT  +PP ++LGYHQ RW+Y     VLEV + F + ++PCDVIW+DI++ D
Sbjct: 375 VFRQYASLTGTQALPPLFSLGYHQSRWNYRDEADVLEVDQGFDDHNMPCDVIWLDIEHAD 434

Query: 258 GFRCFTFDPERFADPKTLVNELHQIGFKAIWMLDPGIKHEKGYFVYDSGSEKDIWVQKAD 317
           G R FT+DP RF  P  ++  L     K + ++DP IK + GY V++      ++V+  D
Sbjct: 435 GKRYFTWDPTRFPQPLNMLEHLASKRRKLVAIVDPHIKVDSGYRVHEELRNHGLYVKTRD 494

Query: 318 GDHYVGEVWPGPCVFPDFTQAKARSWWANLVKDFVSNGVDG------IWNDMNEPAIFKA 377
           G  Y G  WPG   +PDFT  + R+WW+N+   F  +  +G      +WNDMNEP++F  
Sbjct: 495 GSDYEGWCWPGSASYPDFTNPRMRAWWSNM---FSFDNYEGSAPNLYVWNDMNEPSVFNG 554

Query: 378 VTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMAKSTYEGM-KLAKSDRRPFVLTRAGF 437
              TM +  +H G  E      H   HN+YG+ +  +T +G+ + +    RPFVL+RA F
Sbjct: 555 PEVTMLKDAVHYGGWE------HRDIHNIYGLYVHMATADGLIQRSGGIERPFVLSRAFF 614

Query: 438 IGSQRYAATWTGDNLSSWDHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPKLFGRWMG 497
            GSQR+ A WTGDN + WDHL +SI M L L L G    G D+GGF  N  P+L  RW  
Sbjct: 615 SGSQRFGAVWTGDNTAEWDHLKISIPMCLSLALVGLSFCGADVGGFFKNPEPELLVRWYQ 674

Query: 498 IGALFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLFYMAHTTGVPV 557
           +GA  PF R H+   T   EPW    + ++  R AL +RY LLP  YTLFY AH  G PV
Sbjct: 675 MGAYQPFFRAHAHLDTGRREPWLLASQYQDAIRDALFQRYSLLPFWYTLFYQAHKEGFPV 734

Query: 558 ATPVFFADSRDPKLRKIENSFLLGSILIYSSTLPNQGMHNLNFTLP--KGVWLRFDFDDS 617
             P++     D     IE+ F+LG  L+    + + G H +   LP  + VW        
Sbjct: 735 MRPLWVQYPEDMSTFSIEDQFMLGDALLI-HPVSDAGAHGVQVYLPGQEEVWYDIQSYQK 794

Query: 618 HPDLPALFL-----------QGGSIVPLGPPHQHTGEANPSDDISLLVALDENGKAKGVL 677
           H     L+L           +GG+IVP     + + +    D I+L VAL   G A+G L
Sbjct: 795 HHGPQTLYLPVTLSSIPVFQRGGTIVPRWMRVRRSSDCMKDDPITLFVALSPQGTAQGEL 854

Query: 678 FEDDGDGYGFSLGAYLLTHYVAELESSVVTVQVSRTEGSWTRPKRHLHVQI 709
           F DDG  + +      L    +   S++V+        S   PK HL   I
Sbjct: 855 FLDDGHTFNYQTRHEFLLRRFSFSGSTLVS--------SSADPKGHLETPI 887

BLAST of MC10g1385 vs. ExPASy Swiss-Prot
Match: Q14697 (Neutral alpha-glucosidase AB OS=Homo sapiens OX=9606 GN=GANAB PE=1 SV=3)

HSP 1 Score: 359.4 bits (921), Expect = 1.3e-97
Identity = 191/501 (38.12%), Postives = 271/501 (54.09%), Query Frame = 0

Query: 187 ITFGPFSSPTAVLKAFSSAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFREKDIPC 246
           +  GP  S + V + ++S  GT  +PP ++LGYHQ RW+Y     VLEV + F + ++PC
Sbjct: 366 LLLGP--SISDVFRQYASLTGTQALPPLFSLGYHQSRWNYRDEADVLEVDQGFDDHNLPC 425

Query: 247 DVIWMDIDYMDGFRCFTFDPERFADPKTLVNELHQIGFKAIWMLDPGIKHEKGYFVYDSG 306
           DVIW+DI++ DG R FT+DP RF  P+T++  L     K + ++DP IK + GY V++  
Sbjct: 426 DVIWLDIEHADGKRYFTWDPSRFPQPRTMLERLASKRRKLVAIVDPHIKVDSGYRVHEEL 485

Query: 307 SEKDIWVQKADGDHYVGEVWPGPCVFPDFTQAKARSWWANLVKDFVSNGVDG------IW 366
               ++V+  DG  Y G  WPG   +PDFT    R+WWAN+   F  +  +G      +W
Sbjct: 486 RNLGLYVKTRDGSDYEGWCWPGSAGYPDFTNPTMRAWWANM---FSYDNYEGSAPNLFVW 545

Query: 367 NDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMAKSTYEGMKLAKSD- 426
           NDMNEP++F       PE  + +  + +GG + H   HN+YG+ +  +T +G++      
Sbjct: 546 NDMNEPSVFNG-----PEVTMLKDAQHYGGWE-HRDVHNIYGLYVHMATADGLRQRSGGM 605

Query: 427 RRPFVLTRAGFIGSQRYAATWTGDNLSSWDHLHMSISMVLQLGLSGQPLSGPDIGGFAGN 486
            RPFVL RA F GSQR+ A WTGDN + WDHL +SI M L LGL G    G D+GGF  N
Sbjct: 606 ERPFVLARAFFAGSQRFGAVWTGDNTAEWDHLKISIPMCLSLGLVGLSFCGADVGGFFKN 665

Query: 487 ATPKLFGRWMGIGALFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTL 546
             P+L  RW  +GA  PF R H+   T   EPW    +  ++ R AL +RY LLP  YTL
Sbjct: 666 PEPELLVRWYQMGAYQPFFRAHAHLDTGRREPWLLPSQHNDIIRDALGQRYSLLPFWYTL 725

Query: 547 FYMAHTTGVPVATPVFFADSRDPKLRKIENSFLLGSILIYSSTLPNQGMHNLNFTLP--K 606
            Y AH  G+PV  P++    +D     I++ +LLG  L+    + + G H +   LP   
Sbjct: 726 LYQAHREGIPVMRPLWVQYPQDVTTFNIDDQYLLGDALLV-HPVSDSGAHGVQVYLPGQG 785

Query: 607 GVWLRFDFDDSHPDLPALFL-----------QGGSIVPLGPPHQHTGEANPSDDISLLVA 666
            VW        H     L+L           +GG+IVP     + + E    D I+L VA
Sbjct: 786 EVWYDIQSYQKHHGPQTLYLPVTLSSIPVFQRGGTIVPRWMRVRRSSECMKDDPITLFVA 845

Query: 667 LDENGKAKGVLFEDDGDGYGF 668
           L   G A+G LF DDG  + +
Sbjct: 846 LSPQGTAQGELFLDDGHTFNY 854

BLAST of MC10g1385 vs. NCBI nr
Match: XP_022145307.1 (uncharacterized protein LOC111014792 [Momordica charantia])

HSP 1 Score: 2095 bits (5428), Expect = 0.0
Identity = 1005/1005 (100.00%), Postives = 1005/1005 (100.00%), Query Frame = 0

Query: 1    RRKKINQKLISGSFTCKMADGKERGITTDTISGNMIFEPILEDGVFRFDCSSHDRAAAYP 60
            RRKKINQKLISGSFTCKMADGKERGITTDTISGNMIFEPILEDGVFRFDCSSHDRAAAYP
Sbjct: 53   RRKKINQKLISGSFTCKMADGKERGITTDTISGNMIFEPILEDGVFRFDCSSHDRAAAYP 112

Query: 61   SFSFLRSEDRDTPISSQKLPTYVPVFECLLGQQIVKLELPAGTSFYGTGEVSGQLERTGK 120
            SFSFLRSEDRDTPISSQKLPTYVPVFECLLGQQIVKLELPAGTSFYGTGEVSGQLERTGK
Sbjct: 113  SFSFLRSEDRDTPISSQKLPTYVPVFECLLGQQIVKLELPAGTSFYGTGEVSGQLERTGK 172

Query: 121  RIFTWNTDAYGYGAGTTSLYQAHPWVLAILPNGQALGVLADTSLRCEIDLRKDSIIQFVA 180
            RIFTWNTDAYGYGAGTTSLYQAHPWVLAILPNGQALGVLADTSLRCEIDLRKDSIIQFVA
Sbjct: 173  RIFTWNTDAYGYGAGTTSLYQAHPWVLAILPNGQALGVLADTSLRCEIDLRKDSIIQFVA 232

Query: 181  PSSYPVITFGPFSSPTAVLKAFSSAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR 240
            PSSYPVITFGPFSSPTAVLKAFSSAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR
Sbjct: 233  PSSYPVITFGPFSSPTAVLKAFSSAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR 292

Query: 241  EKDIPCDVIWMDIDYMDGFRCFTFDPERFADPKTLVNELHQIGFKAIWMLDPGIKHEKGY 300
            EKDIPCDVIWMDIDYMDGFRCFTFDPERFADPKTLVNELHQIGFKAIWMLDPGIKHEKGY
Sbjct: 293  EKDIPCDVIWMDIDYMDGFRCFTFDPERFADPKTLVNELHQIGFKAIWMLDPGIKHEKGY 352

Query: 301  FVYDSGSEKDIWVQKADGDHYVGEVWPGPCVFPDFTQAKARSWWANLVKDFVSNGVDGIW 360
            FVYDSGSEKDIWVQKADGDHYVGEVWPGPCVFPDFTQAKARSWWANLVKDFVSNGVDGIW
Sbjct: 353  FVYDSGSEKDIWVQKADGDHYVGEVWPGPCVFPDFTQAKARSWWANLVKDFVSNGVDGIW 412

Query: 361  NDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMAKSTYEGMKLAKSDR 420
            NDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMAKSTYEGMKLAKSDR
Sbjct: 413  NDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMAKSTYEGMKLAKSDR 472

Query: 421  RPFVLTRAGFIGSQRYAATWTGDNLSSWDHLHMSISMVLQLGLSGQPLSGPDIGGFAGNA 480
            RPFVLTRAGFIGSQRYAATWTGDNLSSWDHLHMSISMVLQLGLSGQPLSGPDIGGFAGNA
Sbjct: 473  RPFVLTRAGFIGSQRYAATWTGDNLSSWDHLHMSISMVLQLGLSGQPLSGPDIGGFAGNA 532

Query: 481  TPKLFGRWMGIGALFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF 540
            TPKLFGRWMGIGALFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF
Sbjct: 533  TPKLFGRWMGIGALFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF 592

Query: 541  YMAHTTGVPVATPVFFADSRDPKLRKIENSFLLGSILIYSSTLPNQGMHNLNFTLPKGVW 600
            YMAHTTGVPVATPVFFADSRDPKLRKIENSFLLGSILIYSSTLPNQGMHNLNFTLPKGVW
Sbjct: 593  YMAHTTGVPVATPVFFADSRDPKLRKIENSFLLGSILIYSSTLPNQGMHNLNFTLPKGVW 652

Query: 601  LRFDFDDSHPDLPALFLQGGSIVPLGPPHQHTGEANPSDDISLLVALDENGKAKGVLFED 660
            LRFDFDDSHPDLPALFLQGGSIVPLGPPHQHTGEANPSDDISLLVALDENGKAKGVLFED
Sbjct: 653  LRFDFDDSHPDLPALFLQGGSIVPLGPPHQHTGEANPSDDISLLVALDENGKAKGVLFED 712

Query: 661  DGDGYGFSLGAYLLTHYVAELESSVVTVQVSRTEGSWTRPKRHLHVQILLGGGAKIEAWG 720
            DGDGYGFSLGAYLLTHYVAELESSVVTVQVSRTEGSWTRPKRHLHVQILLGGGAKIEAWG
Sbjct: 713  DGDGYGFSLGAYLLTHYVAELESSVVTVQVSRTEGSWTRPKRHLHVQILLGGGAKIEAWG 772

Query: 721  IDGDVLQVTLPSEQQVADLVAISEKEYHHRLEIAKTLPDVEDVPEHKGVALSKIPVELKG 780
            IDGDVLQVTLPSEQQVADLVAISEKEYHHRLEIAKTLPDVEDVPEHKGVALSKIPVELKG
Sbjct: 773  IDGDVLQVTLPSEQQVADLVAISEKEYHHRLEIAKTLPDVEDVPEHKGVALSKIPVELKG 832

Query: 781  AHWSVKVIPWIGGRIVSMTHLPSGIQWLQSKIDINGYEEFSGTEYLSAGCTEEYSIIDRN 840
            AHWSVKVIPWIGGRIVSMTHLPSGIQWLQSKIDINGYEEFSGTEYLSAGCTEEYSIIDRN
Sbjct: 833  AHWSVKVIPWIGGRIVSMTHLPSGIQWLQSKIDINGYEEFSGTEYLSAGCTEEYSIIDRN 892

Query: 841  FEHAGDEESLELEGDIDGGLALRRKIYIPKDYARVLQINSSIIAVKVGAGSGGFSRLVCL 900
            FEHAGDEESLELEGDIDGGLALRRKIYIPKDYARVLQINSSIIAVKVGAGSGGFSRLVCL
Sbjct: 893  FEHAGDEESLELEGDIDGGLALRRKIYIPKDYARVLQINSSIIAVKVGAGSGGFSRLVCL 952

Query: 901  RVHPMFNLLHPTESFISFTSINGSVHEIWPESGEQFFEGDLLPNGEWKLVDKCLGLALVN 960
            RVHPMFNLLHPTESFISFTSINGSVHEIWPESGEQFFEGDLLPNGEWKLVDKCLGLALVN
Sbjct: 953  RVHPMFNLLHPTESFISFTSINGSVHEIWPESGEQFFEGDLLPNGEWKLVDKCLGLALVN 1012

Query: 961  KFNIKEVYKSFIHWGTGTVNLELWSEQRPVSKQTPIRISHSYEVI 1005
            KFNIKEVYKSFIHWGTGTVNLELWSEQRPVSKQTPIRISHSYEVI
Sbjct: 1013 KFNIKEVYKSFIHWGTGTVNLELWSEQRPVSKQTPIRISHSYEVI 1057

BLAST of MC10g1385 vs. NCBI nr
Match: XP_022965368.1 (uncharacterized protein LOC111465248 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1924 bits (4984), Expect = 0.0
Identity = 901/1005 (89.65%), Postives = 963/1005 (95.82%), Query Frame = 0

Query: 1    RRKKINQKLISGSFTCKMADGKERGITTDTISGNMIFEPILEDGVFRFDCSSHDRAAAYP 60
            RRK  N+KLISG FTCKM + KE+GIT DTISGNMIFEPILEDGVFRFDCS++DRAAAYP
Sbjct: 54   RRKWTNKKLISGKFTCKMGNTKEKGITIDTISGNMIFEPILEDGVFRFDCSANDRAAAYP 113

Query: 61   SFSFLRSEDRDTPISSQKLPTYVPVFECLLGQQIVKLELPAGTSFYGTGEVSGQLERTGK 120
            SFSF++S+DRDTPISSQKL TYVPVFEC LGQQIVKL+LPAGTSFYGTGEVSGQLERTGK
Sbjct: 114  SFSFIKSKDRDTPISSQKLATYVPVFECHLGQQIVKLQLPAGTSFYGTGEVSGQLERTGK 173

Query: 121  RIFTWNTDAYGYGAGTTSLYQAHPWVLAILPNGQALGVLADTSLRCEIDLRKDSIIQFVA 180
            RIFTWNTDAYGYG+GTTSLYQ+HPWVLAILPNG+ALG+LADT LRCEIDLR+DSIIQF+A
Sbjct: 174  RIFTWNTDAYGYGSGTTSLYQSHPWVLAILPNGEALGILADTFLRCEIDLREDSIIQFIA 233

Query: 181  PSSYPVITFGPFSSPTAVLKAFSSAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR 240
            PSSYPVITFGPFSSP+AVLK+FS AVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR
Sbjct: 234  PSSYPVITFGPFSSPSAVLKSFSKAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR 293

Query: 241  EKDIPCDVIWMDIDYMDGFRCFTFDPERFADPKTLVNELHQIGFKAIWMLDPGIKHEKGY 300
            EK+IPCDVIWMDIDYMDGFRCFTFDPERF DPKTLVN+LHQIG KAIWMLDPGIKHEKGY
Sbjct: 294  EKNIPCDVIWMDIDYMDGFRCFTFDPERFTDPKTLVNDLHQIGIKAIWMLDPGIKHEKGY 353

Query: 301  FVYDSGSEKDIWVQKADGDHYVGEVWPGPCVFPDFTQAKARSWWANLVKDFVSNGVDGIW 360
            FVYDSGSEK++WVQKA+G+ YVGEVWPGPCVFP+FTQAKAR WWANLVKDFVSNGVDGIW
Sbjct: 354  FVYDSGSEKNVWVQKANGEPYVGEVWPGPCVFPEFTQAKARDWWANLVKDFVSNGVDGIW 413

Query: 361  NDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMAKSTYEGMKLAKSDR 420
            NDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMA+STYEGMK A S+R
Sbjct: 414  NDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMARSTYEGMKSANSER 473

Query: 421  RPFVLTRAGFIGSQRYAATWTGDNLSSWDHLHMSISMVLQLGLSGQPLSGPDIGGFAGNA 480
            RPFVLTRAGF+GSQ+YAATWTGDN+SSW+HLHMSISM+LQLGLSGQPLSGPDIGGFAGNA
Sbjct: 474  RPFVLTRAGFVGSQKYAATWTGDNISSWEHLHMSISMILQLGLSGQPLSGPDIGGFAGNA 533

Query: 481  TPKLFGRWMGIGALFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF 540
            TP+LFGRWMGIGA+FPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF
Sbjct: 534  TPRLFGRWMGIGAMFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF 593

Query: 541  YMAHTTGVPVATPVFFADSRDPKLRKIENSFLLGSILIYSSTLPNQGMHNLNFTLPKGVW 600
            YMAHTTGVPVATPVFFAD +D  LRKIENSFLLGSILIYSSTLPNQG++NLN TLPKG+W
Sbjct: 594  YMAHTTGVPVATPVFFADPKDANLRKIENSFLLGSILIYSSTLPNQGINNLNLTLPKGIW 653

Query: 601  LRFDFDDSHPDLPALFLQGGSIVPLGPPHQHTGEANPSDDISLLVALDENGKAKGVLFED 660
             RFDFDDSHPDLP LFLQGGSIVPLGP HQHTGEANPSDD+SLLVALDENGKAKGVL+ED
Sbjct: 654  SRFDFDDSHPDLPVLFLQGGSIVPLGPAHQHTGEANPSDDVSLLVALDENGKAKGVLYED 713

Query: 661  DGDGYGFSLGAYLLTHYVAELESSVVTVQVSRTEGSWTRPKRHLHVQILLGGGAKIEAWG 720
            DGDGYGF+LGAYLLTHYVAELESSVVTVQVSRTEGSWTRPKR LHVQILLGGGAKI+AWG
Sbjct: 714  DGDGYGFTLGAYLLTHYVAELESSVVTVQVSRTEGSWTRPKRRLHVQILLGGGAKIDAWG 773

Query: 721  IDGDVLQVTLPSEQQVADLVAISEKEYHHRLEIAKTLPDVEDVPEHKGVALSKIPVELKG 780
             DG+VLQVTLPSEQ+ ADL+AISE+EYHHRLE AKTL DVE V EHKGV+LSK P+ELKG
Sbjct: 774  TDGEVLQVTLPSEQEAADLIAISEREYHHRLERAKTLQDVEGVSEHKGVSLSKTPIELKG 833

Query: 781  AHWSVKVIPWIGGRIVSMTHLPSGIQWLQSKIDINGYEEFSGTEYLSAGCTEEYSIIDRN 840
             HWSVKV+PWIGGRI+SMTHLPSGIQWLQ KIDINGYEEFSGTEY SAGCTEEYSIIDR 
Sbjct: 834  GHWSVKVVPWIGGRIISMTHLPSGIQWLQGKIDINGYEEFSGTEYRSAGCTEEYSIIDRK 893

Query: 841  FEHAGDEESLELEGDIDGGLALRRKIYIPKDYARVLQINSSIIAVKVGAGSGGFSRLVCL 900
            FE  GDEESLELEGDIDGGLALRRKIYI K+ A+ LQINSSI+AVKVGAGSGGFSRLVCL
Sbjct: 894  FELDGDEESLELEGDIDGGLALRRKIYISKEDAKALQINSSIVAVKVGAGSGGFSRLVCL 953

Query: 901  RVHPMFNLLHPTESFISFTSINGSVHEIWPESGEQFFEGDLLPNGEWKLVDKCLGLALVN 960
            RVHPMFNLLHP+ESFISFTS++GSVHEIWPESGEQ+FEGDLLPNGEWKLVDKCLGLALVN
Sbjct: 954  RVHPMFNLLHPSESFISFTSVDGSVHEIWPESGEQYFEGDLLPNGEWKLVDKCLGLALVN 1013

Query: 961  KFNIKEVYKSFIHWGTGTVNLELWSEQRPVSKQTPIRISHSYEVI 1005
            KFN+KEVYK F++WGTGTVNLELWSEQRPVSK+TP+RI HSYEV+
Sbjct: 1014 KFNVKEVYKCFVYWGTGTVNLELWSEQRPVSKETPLRILHSYEVM 1058

BLAST of MC10g1385 vs. NCBI nr
Match: XP_023552270.1 (uncharacterized protein LOC111809987 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1920 bits (4975), Expect = 0.0
Identity = 899/1005 (89.45%), Postives = 960/1005 (95.52%), Query Frame = 0

Query: 1    RRKKINQKLISGSFTCKMADGKERGITTDTISGNMIFEPILEDGVFRFDCSSHDRAAAYP 60
            RRK  N+KLISG FTCKM + KE+GIT DT+SGNMIFEPILEDGVFRFDCS++DRAAAYP
Sbjct: 54   RRKWTNKKLISGKFTCKMGNTKEKGITIDTLSGNMIFEPILEDGVFRFDCSANDRAAAYP 113

Query: 61   SFSFLRSEDRDTPISSQKLPTYVPVFECLLGQQIVKLELPAGTSFYGTGEVSGQLERTGK 120
            SFSF++S+DRDTPISSQKL TYVPVFEC LGQQIVKL+LPAGTSFYGTGEVSGQLERTGK
Sbjct: 114  SFSFIKSKDRDTPISSQKLATYVPVFECHLGQQIVKLQLPAGTSFYGTGEVSGQLERTGK 173

Query: 121  RIFTWNTDAYGYGAGTTSLYQAHPWVLAILPNGQALGVLADTSLRCEIDLRKDSIIQFVA 180
            RIFTWNTDAYGYG+GTTSLYQ+HPWVLAILPNG+ALGVLADTSLRCEIDLR+DSII F+A
Sbjct: 174  RIFTWNTDAYGYGSGTTSLYQSHPWVLAILPNGEALGVLADTSLRCEIDLREDSIIHFIA 233

Query: 181  PSSYPVITFGPFSSPTAVLKAFSSAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR 240
            PSSYPVITFGPFSSP+AVLK+FS AVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR
Sbjct: 234  PSSYPVITFGPFSSPSAVLKSFSKAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR 293

Query: 241  EKDIPCDVIWMDIDYMDGFRCFTFDPERFADPKTLVNELHQIGFKAIWMLDPGIKHEKGY 300
            EK+IPCDVIWMDIDYMDGFRCFTFDPERFADPKTLVN+LHQIG KAIWMLDPGIKHEKGY
Sbjct: 294  EKNIPCDVIWMDIDYMDGFRCFTFDPERFADPKTLVNDLHQIGIKAIWMLDPGIKHEKGY 353

Query: 301  FVYDSGSEKDIWVQKADGDHYVGEVWPGPCVFPDFTQAKARSWWANLVKDFVSNGVDGIW 360
            FVYDSGSEK++WVQKA+G+ YVGEVWPGPCVFP+FTQAKAR WWANLVKDFVSNGVDGIW
Sbjct: 354  FVYDSGSEKNVWVQKANGEPYVGEVWPGPCVFPEFTQAKARDWWANLVKDFVSNGVDGIW 413

Query: 361  NDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMAKSTYEGMKLAKSDR 420
            NDMNEPAIFK VTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMA+STYEGMK A S+R
Sbjct: 414  NDMNEPAIFKTVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMARSTYEGMKSANSER 473

Query: 421  RPFVLTRAGFIGSQRYAATWTGDNLSSWDHLHMSISMVLQLGLSGQPLSGPDIGGFAGNA 480
            RPFVLTRAGF+GSQ+YAATWTGDN+SSW+HLHMSISM+LQLGLSGQPLSGPDIGGFAGNA
Sbjct: 474  RPFVLTRAGFVGSQKYAATWTGDNISSWEHLHMSISMILQLGLSGQPLSGPDIGGFAGNA 533

Query: 481  TPKLFGRWMGIGALFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF 540
            TP+LFGRWMGIGA+FPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF
Sbjct: 534  TPRLFGRWMGIGAMFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF 593

Query: 541  YMAHTTGVPVATPVFFADSRDPKLRKIENSFLLGSILIYSSTLPNQGMHNLNFTLPKGVW 600
            YMAHTTGVPVATPVFFAD +D  LRKIENSFLLGSILIYSSTLPNQG++NLN TLPKG+W
Sbjct: 594  YMAHTTGVPVATPVFFADPKDANLRKIENSFLLGSILIYSSTLPNQGINNLNLTLPKGIW 653

Query: 601  LRFDFDDSHPDLPALFLQGGSIVPLGPPHQHTGEANPSDDISLLVALDENGKAKGVLFED 660
             RFDFDDSHPDLP LFLQGGSIVPLGP HQHTGEANPSDD+SLLVALDENGK KGVL+ED
Sbjct: 654  SRFDFDDSHPDLPVLFLQGGSIVPLGPAHQHTGEANPSDDVSLLVALDENGKGKGVLYED 713

Query: 661  DGDGYGFSLGAYLLTHYVAELESSVVTVQVSRTEGSWTRPKRHLHVQILLGGGAKIEAWG 720
            DGDGYGF+LGAYLLTHYVAELESSVVTVQVSRTEGSWTRPKR LHVQILLGGGAKI+AWG
Sbjct: 714  DGDGYGFTLGAYLLTHYVAELESSVVTVQVSRTEGSWTRPKRRLHVQILLGGGAKIDAWG 773

Query: 721  IDGDVLQVTLPSEQQVADLVAISEKEYHHRLEIAKTLPDVEDVPEHKGVALSKIPVELKG 780
             DG+VLQVTLPSEQ+ ADL+AISE+EY HRLE AKTL D E V EHKGV+LSK P+ELKG
Sbjct: 774  TDGEVLQVTLPSEQEAADLIAISEREYRHRLESAKTLQDHEGVSEHKGVSLSKTPIELKG 833

Query: 781  AHWSVKVIPWIGGRIVSMTHLPSGIQWLQSKIDINGYEEFSGTEYLSAGCTEEYSIIDRN 840
             HWSVKV+PWIGGRI+SMTHLPSGIQWLQ KIDINGYEEFSGTEY S GCTEEYSIIDR 
Sbjct: 834  GHWSVKVVPWIGGRIISMTHLPSGIQWLQGKIDINGYEEFSGTEYRSPGCTEEYSIIDRK 893

Query: 841  FEHAGDEESLELEGDIDGGLALRRKIYIPKDYARVLQINSSIIAVKVGAGSGGFSRLVCL 900
             EH GDEESLELEGDIDGGLALRRKIYI K+ A+ LQINSSI+AVKVGAGSGGFSRLVCL
Sbjct: 894  IEHDGDEESLELEGDIDGGLALRRKIYISKEDAKALQINSSIVAVKVGAGSGGFSRLVCL 953

Query: 901  RVHPMFNLLHPTESFISFTSINGSVHEIWPESGEQFFEGDLLPNGEWKLVDKCLGLALVN 960
            RVHPMFNLLHP+ESFISFTS++GSVHEIWPESGE++FEGDLLPNGEWKLVDKCLGLALVN
Sbjct: 954  RVHPMFNLLHPSESFISFTSVDGSVHEIWPESGEKYFEGDLLPNGEWKLVDKCLGLALVN 1013

Query: 961  KFNIKEVYKSFIHWGTGTVNLELWSEQRPVSKQTPIRISHSYEVI 1005
            KFNIKEVYK F+HWGTGTVNLELWSEQRPVSK+TP+RISHSYEV+
Sbjct: 1014 KFNIKEVYKCFVHWGTGTVNLELWSEQRPVSKETPLRISHSYEVM 1058

BLAST of MC10g1385 vs. NCBI nr
Match: XP_022965369.1 (uncharacterized protein LOC111465248 isoform X2 [Cucurbita maxima])

HSP 1 Score: 1917 bits (4965), Expect = 0.0
Identity = 899/1005 (89.45%), Postives = 963/1005 (95.82%), Query Frame = 0

Query: 1    RRKKINQKLISGSFTCKMADGKERGITTDTISGNMIFEPILEDGVFRFDCSSHDRAAAYP 60
            RRK  N+KLISG FTCKM + KE+GIT DTISGNMIFEPILEDGVFRFDCS++DRAAAYP
Sbjct: 54   RRKWTNKKLISGKFTCKMGNTKEKGITIDTISGNMIFEPILEDGVFRFDCSANDRAAAYP 113

Query: 61   SFSFLRSEDRDTPISSQKLPTYVPVFECLLGQQIVKLELPAGTSFYGTGEVSGQLERTGK 120
            SFSF++S+DRDTPISSQKL TYVPVFEC LGQQIVKL+LPAGTSFYGTGEVSGQLERTGK
Sbjct: 114  SFSFIKSKDRDTPISSQKLATYVPVFECHLGQQIVKLQLPAGTSFYGTGEVSGQLERTGK 173

Query: 121  RIFTWNTDAYGYGAGTTSLYQAHPWVLAILPNGQALGVLADTSLRCEIDLRKDSIIQFVA 180
            RIFTWNTDAYGYG+GTTSLYQ+HPWVLAILPNG+ALG+LADT LRCEIDLR+DSIIQF+A
Sbjct: 174  RIFTWNTDAYGYGSGTTSLYQSHPWVLAILPNGEALGILADTFLRCEIDLREDSIIQFIA 233

Query: 181  PSSYPVITFGPFSSPTAVLKAFSSAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR 240
            PSSYPVITFGPFSSP+AVLK+FS AVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR
Sbjct: 234  PSSYPVITFGPFSSPSAVLKSFSKAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR 293

Query: 241  EKDIPCDVIWMDIDYMDGFRCFTFDPERFADPKTLVNELHQIGFKAIWMLDPGIKHEKGY 300
            EK+IPCDVIWMDIDYMDGFRCFTFDPERF DPKTLVN+LHQIG KAIWMLDPGIKHEKGY
Sbjct: 294  EKNIPCDVIWMDIDYMDGFRCFTFDPERFTDPKTLVNDLHQIGIKAIWMLDPGIKHEKGY 353

Query: 301  FVYDSGSEKDIWVQKADGDHYVGEVWPGPCVFPDFTQAKARSWWANLVKDFVSNGVDGIW 360
            FVYDSGSEK++WVQKA+G+ YVGEVWPGPCVFP+FTQAKAR WWANLVKDFVSNGVDGIW
Sbjct: 354  FVYDSGSEKNVWVQKANGEPYVGEVWPGPCVFPEFTQAKARDWWANLVKDFVSNGVDGIW 413

Query: 361  NDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMAKSTYEGMKLAKSDR 420
            NDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMA+STYEGMK A S+R
Sbjct: 414  NDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMARSTYEGMKSANSER 473

Query: 421  RPFVLTRAGFIGSQRYAATWTGDNLSSWDHLHMSISMVLQLGLSGQPLSGPDIGGFAGNA 480
            RPFVLTRAGF+GSQ+YAATWTGDN+SSW+HLHMSISM+LQLGLSGQPLSGPDIGGFAGNA
Sbjct: 474  RPFVLTRAGFVGSQKYAATWTGDNISSWEHLHMSISMILQLGLSGQPLSGPDIGGFAGNA 533

Query: 481  TPKLFGRWMGIGALFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF 540
            TP+LFGRWMGIGA+FPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF
Sbjct: 534  TPRLFGRWMGIGAMFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF 593

Query: 541  YMAHTTGVPVATPVFFADSRDPKLRKIENSFLLGSILIYSSTLPNQGMHNLNFTLPKGVW 600
            YMAHTTGVPVATPVFFAD +D  LRKIENSFLLGSILIYSSTLPNQG++NLN TLPKG+W
Sbjct: 594  YMAHTTGVPVATPVFFADPKDANLRKIENSFLLGSILIYSSTLPNQGINNLNLTLPKGIW 653

Query: 601  LRFDFDDSHPDLPALFLQGGSIVPLGPPHQHTGEANPSDDISLLVALDENGKAKGVLFED 660
             RFDFDDSHPDLP LFLQGGSIVPLGP HQHTGEANPSDD+SLLVALDENGKAKGVL+ED
Sbjct: 654  SRFDFDDSHPDLPVLFLQGGSIVPLGPAHQHTGEANPSDDVSLLVALDENGKAKGVLYED 713

Query: 661  DGDGYGFSLGAYLLTHYVAELESSVVTVQVSRTEGSWTRPKRHLHVQILLGGGAKIEAWG 720
            DGDGYGF+LGAYLLTHYVAELESSVVTVQVSRTEGSWTRPKR LHVQILLGGGAKI+AWG
Sbjct: 714  DGDGYGFTLGAYLLTHYVAELESSVVTVQVSRTEGSWTRPKRRLHVQILLGGGAKIDAWG 773

Query: 721  IDGDVLQVTLPSEQQVADLVAISEKEYHHRLEIAKTLPDVEDVPEHKGVALSKIPVELKG 780
             DG+VLQVTLPSEQ+ ADL+AISE+EYHHRLE AKTL DVE V EHKGV+LSK P+ELKG
Sbjct: 774  TDGEVLQVTLPSEQEAADLIAISEREYHHRLERAKTLQDVEGVSEHKGVSLSKTPIELKG 833

Query: 781  AHWSVKVIPWIGGRIVSMTHLPSGIQWLQSKIDINGYEEFSGTEYLSAGCTEEYSIIDRN 840
             HWSVKV+PWIGGRI+SMTHLPSGIQWLQ KIDINGYEEFSGTEY SAGCTEEYSII++ 
Sbjct: 834  GHWSVKVVPWIGGRIISMTHLPSGIQWLQGKIDINGYEEFSGTEYRSAGCTEEYSIIEK- 893

Query: 841  FEHAGDEESLELEGDIDGGLALRRKIYIPKDYARVLQINSSIIAVKVGAGSGGFSRLVCL 900
            FE  GDEESLELEGDIDGGLALRRKIYI K+ A+ LQINSSI+AVKVGAGSGGFSRLVCL
Sbjct: 894  FELDGDEESLELEGDIDGGLALRRKIYISKEDAKALQINSSIVAVKVGAGSGGFSRLVCL 953

Query: 901  RVHPMFNLLHPTESFISFTSINGSVHEIWPESGEQFFEGDLLPNGEWKLVDKCLGLALVN 960
            RVHPMFNLLHP+ESFISFTS++GSVHEIWPESGEQ+FEGDLLPNGEWKLVDKCLGLALVN
Sbjct: 954  RVHPMFNLLHPSESFISFTSVDGSVHEIWPESGEQYFEGDLLPNGEWKLVDKCLGLALVN 1013

Query: 961  KFNIKEVYKSFIHWGTGTVNLELWSEQRPVSKQTPIRISHSYEVI 1005
            KFN+KEVYK F++WGTGTVNLELWSEQRPVSK+TP+RI HSYEV+
Sbjct: 1014 KFNVKEVYKCFVYWGTGTVNLELWSEQRPVSKETPLRILHSYEVM 1057

BLAST of MC10g1385 vs. NCBI nr
Match: XP_038876569.1 (alpha-glucosidase 2 [Benincasa hispida])

HSP 1 Score: 1916 bits (4964), Expect = 0.0
Identity = 902/1006 (89.66%), Postives = 963/1006 (95.73%), Query Frame = 0

Query: 1    RRKKINQKLISGSFTCKMADGKERGITTDT-ISGNMIFEPILEDGVFRFDCSSHDRAAAY 60
            RRK+ N+KLIS +FTCKM + KE+G T DT ISGNMIFEPILEDGVFRFDCS++DRAAAY
Sbjct: 55   RRKRTNKKLISENFTCKMGNVKEKGTTKDTTISGNMIFEPILEDGVFRFDCSANDRAAAY 114

Query: 61   PSFSFLRSEDRDTPISSQKLPTYVPVFECLLGQQIVKLELPAGTSFYGTGEVSGQLERTG 120
            PSFSFL+S+DRDTPISSQKLPTY+PVFECLLGQQIVKLELPAGTS YGTGEVSGQLERTG
Sbjct: 115  PSFSFLKSKDRDTPISSQKLPTYIPVFECLLGQQIVKLELPAGTSLYGTGEVSGQLERTG 174

Query: 121  KRIFTWNTDAYGYGAGTTSLYQAHPWVLAILPNGQALGVLADTSLRCEIDLRKDSIIQFV 180
            KRIFTWNTDAYGYG+GTTSLYQ+HPWVLAILPNG+ALGVLADTSLRCEIDLR+DS+IQF+
Sbjct: 175  KRIFTWNTDAYGYGSGTTSLYQSHPWVLAILPNGEALGVLADTSLRCEIDLREDSVIQFI 234

Query: 181  APSSYPVITFGPFSSPTAVLKAFSSAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTF 240
            APSSYPVITFGPFSSP A LK+FS AVGTVF+PPKWALGYHQCRWSYDSADRVLEVSRTF
Sbjct: 235  APSSYPVITFGPFSSPAAALKSFSRAVGTVFLPPKWALGYHQCRWSYDSADRVLEVSRTF 294

Query: 241  REKDIPCDVIWMDIDYMDGFRCFTFDPERFADPKTLVNELHQIGFKAIWMLDPGIKHEKG 300
            R+KDIPCDVIWMDIDYMDGFRCFTFDPERFADPKTLV++LHQIGFKAIWMLDPGIKHEKG
Sbjct: 295  RKKDIPCDVIWMDIDYMDGFRCFTFDPERFADPKTLVDDLHQIGFKAIWMLDPGIKHEKG 354

Query: 301  YFVYDSGSEKDIWVQKADGDHYVGEVWPGPCVFPDFTQAKARSWWANLVKDFVSNGVDGI 360
            YFVYDSGSEKD+WVQKADG+ YVGEVWPGPCVFP+FTQAKARSWWANLVKDFVSNGVDGI
Sbjct: 355  YFVYDSGSEKDVWVQKADGEPYVGEVWPGPCVFPEFTQAKARSWWANLVKDFVSNGVDGI 414

Query: 361  WNDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMAKSTYEGMKLAKSD 420
            WNDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMA+STYEGMK+A S+
Sbjct: 415  WNDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMARSTYEGMKMANSE 474

Query: 421  RRPFVLTRAGFIGSQRYAATWTGDNLSSWDHLHMSISMVLQLGLSGQPLSGPDIGGFAGN 480
            RRPFVLTRAGFIGSQ+YAATWTGDN+SSW+HLHMSISM+LQLGLSGQPLSGPDIGGFAGN
Sbjct: 475  RRPFVLTRAGFIGSQKYAATWTGDNISSWEHLHMSISMILQLGLSGQPLSGPDIGGFAGN 534

Query: 481  ATPKLFGRWMGIGALFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTL 540
            ATP+LFGRWMGIGA+FPFCRGHSET TADHEPWSFGEECEEVCRLALKRRYRLLPHIYTL
Sbjct: 535  ATPRLFGRWMGIGAMFPFCRGHSETRTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTL 594

Query: 541  FYMAHTTGVPVATPVFFADSRDPKLRKIENSFLLGSILIYSSTLPNQGMHNLNFTLPKGV 600
            FY+AHTTGVPVATPVFFAD +DP LRKIENSFLLGSILIYSST PNQ + N N TLPKG+
Sbjct: 595  FYIAHTTGVPVATPVFFADPKDPNLRKIENSFLLGSILIYSSTFPNQRIDNSNLTLPKGI 654

Query: 601  WLRFDFDDSHPDLPALFLQGGSIVPLGPPHQHTGEANPSDDISLLVALDENGKAKGVLFE 660
            W RFDFDDSHPDLPALFLQGGSIVPLGP HQHTGEA PSDDISLLVALDENGKA+GVLFE
Sbjct: 655  WSRFDFDDSHPDLPALFLQGGSIVPLGPAHQHTGEAKPSDDISLLVALDENGKAEGVLFE 714

Query: 661  DDGDGYGFSLGAYLLTHYVAELESSVVTVQVSRTEGSWTRPKRHLHVQILLGGGAKIEAW 720
            DDGDGYGFSLGAYLLT YVAEL+SSVVTVQVSRTEGSWTRPKR LHVQILLGGGAKI+AW
Sbjct: 715  DDGDGYGFSLGAYLLTRYVAELKSSVVTVQVSRTEGSWTRPKRRLHVQILLGGGAKIDAW 774

Query: 721  GIDGDVLQVTLPSEQQVADLVAISEKEYHHRLEIAKTLPDVEDVPEHKGVALSKIPVELK 780
            G DG+VLQVT PSEQ+VADLVA SEKE  HRLE AKTL DVE+V EHKGV+LSK P+ELK
Sbjct: 775  GTDGEVLQVTFPSEQEVADLVATSEKECRHRLESAKTLQDVEEVSEHKGVSLSKTPIELK 834

Query: 781  GAHWSVKVIPWIGGRIVSMTHLPSGIQWLQSKIDINGYEEFSGTEYLSAGCTEEYSIIDR 840
            GAHWSVKV+PWIGGRI+SMTHLPSGIQWLQSKI+INGYEEFSGTEY SAGCTEEYSIID+
Sbjct: 835  GAHWSVKVVPWIGGRIISMTHLPSGIQWLQSKIEINGYEEFSGTEYRSAGCTEEYSIIDQ 894

Query: 841  NFEHAGDEESLELEGDIDGGLALRRKIYIPKDYARVLQINSSIIAVKVGAGSGGFSRLVC 900
            NFEHAGDEESLELEGDIDGGL LRRKIYIPK+ A+VL+INSSI+AVKVGAGSGGFSRLVC
Sbjct: 895  NFEHAGDEESLELEGDIDGGLVLRRKIYIPKEDAKVLKINSSIVAVKVGAGSGGFSRLVC 954

Query: 901  LRVHPMFNLLHPTESFISFTSINGSVHEIWPESGEQFFEGDLLPNGEWKLVDKCLGLALV 960
            LRVHPMFNLLHPT+SF++FTSI+GSVHEIWPESGEQ+ EGDLLPNGEWKLVDKCLGLALV
Sbjct: 955  LRVHPMFNLLHPTKSFVTFTSIDGSVHEIWPESGEQYLEGDLLPNGEWKLVDKCLGLALV 1014

Query: 961  NKFNIKEVYKSFIHWGTGTVNLELWSEQRPVSKQTPIRISHSYEVI 1005
            NKFNIKEV K  IHWGTGTVNLELWSEQRPVSK++P+ ISHSYEVI
Sbjct: 1015 NKFNIKEVNKCLIHWGTGTVNLELWSEQRPVSKESPLEISHSYEVI 1060

BLAST of MC10g1385 vs. ExPASy TrEMBL
Match: A0A6J1CW78 (uncharacterized protein LOC111014792 OS=Momordica charantia OX=3673 GN=LOC111014792 PE=3 SV=1)

HSP 1 Score: 2095 bits (5428), Expect = 0.0
Identity = 1005/1005 (100.00%), Postives = 1005/1005 (100.00%), Query Frame = 0

Query: 1    RRKKINQKLISGSFTCKMADGKERGITTDTISGNMIFEPILEDGVFRFDCSSHDRAAAYP 60
            RRKKINQKLISGSFTCKMADGKERGITTDTISGNMIFEPILEDGVFRFDCSSHDRAAAYP
Sbjct: 53   RRKKINQKLISGSFTCKMADGKERGITTDTISGNMIFEPILEDGVFRFDCSSHDRAAAYP 112

Query: 61   SFSFLRSEDRDTPISSQKLPTYVPVFECLLGQQIVKLELPAGTSFYGTGEVSGQLERTGK 120
            SFSFLRSEDRDTPISSQKLPTYVPVFECLLGQQIVKLELPAGTSFYGTGEVSGQLERTGK
Sbjct: 113  SFSFLRSEDRDTPISSQKLPTYVPVFECLLGQQIVKLELPAGTSFYGTGEVSGQLERTGK 172

Query: 121  RIFTWNTDAYGYGAGTTSLYQAHPWVLAILPNGQALGVLADTSLRCEIDLRKDSIIQFVA 180
            RIFTWNTDAYGYGAGTTSLYQAHPWVLAILPNGQALGVLADTSLRCEIDLRKDSIIQFVA
Sbjct: 173  RIFTWNTDAYGYGAGTTSLYQAHPWVLAILPNGQALGVLADTSLRCEIDLRKDSIIQFVA 232

Query: 181  PSSYPVITFGPFSSPTAVLKAFSSAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR 240
            PSSYPVITFGPFSSPTAVLKAFSSAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR
Sbjct: 233  PSSYPVITFGPFSSPTAVLKAFSSAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR 292

Query: 241  EKDIPCDVIWMDIDYMDGFRCFTFDPERFADPKTLVNELHQIGFKAIWMLDPGIKHEKGY 300
            EKDIPCDVIWMDIDYMDGFRCFTFDPERFADPKTLVNELHQIGFKAIWMLDPGIKHEKGY
Sbjct: 293  EKDIPCDVIWMDIDYMDGFRCFTFDPERFADPKTLVNELHQIGFKAIWMLDPGIKHEKGY 352

Query: 301  FVYDSGSEKDIWVQKADGDHYVGEVWPGPCVFPDFTQAKARSWWANLVKDFVSNGVDGIW 360
            FVYDSGSEKDIWVQKADGDHYVGEVWPGPCVFPDFTQAKARSWWANLVKDFVSNGVDGIW
Sbjct: 353  FVYDSGSEKDIWVQKADGDHYVGEVWPGPCVFPDFTQAKARSWWANLVKDFVSNGVDGIW 412

Query: 361  NDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMAKSTYEGMKLAKSDR 420
            NDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMAKSTYEGMKLAKSDR
Sbjct: 413  NDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMAKSTYEGMKLAKSDR 472

Query: 421  RPFVLTRAGFIGSQRYAATWTGDNLSSWDHLHMSISMVLQLGLSGQPLSGPDIGGFAGNA 480
            RPFVLTRAGFIGSQRYAATWTGDNLSSWDHLHMSISMVLQLGLSGQPLSGPDIGGFAGNA
Sbjct: 473  RPFVLTRAGFIGSQRYAATWTGDNLSSWDHLHMSISMVLQLGLSGQPLSGPDIGGFAGNA 532

Query: 481  TPKLFGRWMGIGALFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF 540
            TPKLFGRWMGIGALFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF
Sbjct: 533  TPKLFGRWMGIGALFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF 592

Query: 541  YMAHTTGVPVATPVFFADSRDPKLRKIENSFLLGSILIYSSTLPNQGMHNLNFTLPKGVW 600
            YMAHTTGVPVATPVFFADSRDPKLRKIENSFLLGSILIYSSTLPNQGMHNLNFTLPKGVW
Sbjct: 593  YMAHTTGVPVATPVFFADSRDPKLRKIENSFLLGSILIYSSTLPNQGMHNLNFTLPKGVW 652

Query: 601  LRFDFDDSHPDLPALFLQGGSIVPLGPPHQHTGEANPSDDISLLVALDENGKAKGVLFED 660
            LRFDFDDSHPDLPALFLQGGSIVPLGPPHQHTGEANPSDDISLLVALDENGKAKGVLFED
Sbjct: 653  LRFDFDDSHPDLPALFLQGGSIVPLGPPHQHTGEANPSDDISLLVALDENGKAKGVLFED 712

Query: 661  DGDGYGFSLGAYLLTHYVAELESSVVTVQVSRTEGSWTRPKRHLHVQILLGGGAKIEAWG 720
            DGDGYGFSLGAYLLTHYVAELESSVVTVQVSRTEGSWTRPKRHLHVQILLGGGAKIEAWG
Sbjct: 713  DGDGYGFSLGAYLLTHYVAELESSVVTVQVSRTEGSWTRPKRHLHVQILLGGGAKIEAWG 772

Query: 721  IDGDVLQVTLPSEQQVADLVAISEKEYHHRLEIAKTLPDVEDVPEHKGVALSKIPVELKG 780
            IDGDVLQVTLPSEQQVADLVAISEKEYHHRLEIAKTLPDVEDVPEHKGVALSKIPVELKG
Sbjct: 773  IDGDVLQVTLPSEQQVADLVAISEKEYHHRLEIAKTLPDVEDVPEHKGVALSKIPVELKG 832

Query: 781  AHWSVKVIPWIGGRIVSMTHLPSGIQWLQSKIDINGYEEFSGTEYLSAGCTEEYSIIDRN 840
            AHWSVKVIPWIGGRIVSMTHLPSGIQWLQSKIDINGYEEFSGTEYLSAGCTEEYSIIDRN
Sbjct: 833  AHWSVKVIPWIGGRIVSMTHLPSGIQWLQSKIDINGYEEFSGTEYLSAGCTEEYSIIDRN 892

Query: 841  FEHAGDEESLELEGDIDGGLALRRKIYIPKDYARVLQINSSIIAVKVGAGSGGFSRLVCL 900
            FEHAGDEESLELEGDIDGGLALRRKIYIPKDYARVLQINSSIIAVKVGAGSGGFSRLVCL
Sbjct: 893  FEHAGDEESLELEGDIDGGLALRRKIYIPKDYARVLQINSSIIAVKVGAGSGGFSRLVCL 952

Query: 901  RVHPMFNLLHPTESFISFTSINGSVHEIWPESGEQFFEGDLLPNGEWKLVDKCLGLALVN 960
            RVHPMFNLLHPTESFISFTSINGSVHEIWPESGEQFFEGDLLPNGEWKLVDKCLGLALVN
Sbjct: 953  RVHPMFNLLHPTESFISFTSINGSVHEIWPESGEQFFEGDLLPNGEWKLVDKCLGLALVN 1012

Query: 961  KFNIKEVYKSFIHWGTGTVNLELWSEQRPVSKQTPIRISHSYEVI 1005
            KFNIKEVYKSFIHWGTGTVNLELWSEQRPVSKQTPIRISHSYEVI
Sbjct: 1013 KFNIKEVYKSFIHWGTGTVNLELWSEQRPVSKQTPIRISHSYEVI 1057

BLAST of MC10g1385 vs. ExPASy TrEMBL
Match: A0A6J1HNP7 (uncharacterized protein LOC111465248 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465248 PE=3 SV=1)

HSP 1 Score: 1924 bits (4984), Expect = 0.0
Identity = 901/1005 (89.65%), Postives = 963/1005 (95.82%), Query Frame = 0

Query: 1    RRKKINQKLISGSFTCKMADGKERGITTDTISGNMIFEPILEDGVFRFDCSSHDRAAAYP 60
            RRK  N+KLISG FTCKM + KE+GIT DTISGNMIFEPILEDGVFRFDCS++DRAAAYP
Sbjct: 54   RRKWTNKKLISGKFTCKMGNTKEKGITIDTISGNMIFEPILEDGVFRFDCSANDRAAAYP 113

Query: 61   SFSFLRSEDRDTPISSQKLPTYVPVFECLLGQQIVKLELPAGTSFYGTGEVSGQLERTGK 120
            SFSF++S+DRDTPISSQKL TYVPVFEC LGQQIVKL+LPAGTSFYGTGEVSGQLERTGK
Sbjct: 114  SFSFIKSKDRDTPISSQKLATYVPVFECHLGQQIVKLQLPAGTSFYGTGEVSGQLERTGK 173

Query: 121  RIFTWNTDAYGYGAGTTSLYQAHPWVLAILPNGQALGVLADTSLRCEIDLRKDSIIQFVA 180
            RIFTWNTDAYGYG+GTTSLYQ+HPWVLAILPNG+ALG+LADT LRCEIDLR+DSIIQF+A
Sbjct: 174  RIFTWNTDAYGYGSGTTSLYQSHPWVLAILPNGEALGILADTFLRCEIDLREDSIIQFIA 233

Query: 181  PSSYPVITFGPFSSPTAVLKAFSSAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR 240
            PSSYPVITFGPFSSP+AVLK+FS AVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR
Sbjct: 234  PSSYPVITFGPFSSPSAVLKSFSKAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR 293

Query: 241  EKDIPCDVIWMDIDYMDGFRCFTFDPERFADPKTLVNELHQIGFKAIWMLDPGIKHEKGY 300
            EK+IPCDVIWMDIDYMDGFRCFTFDPERF DPKTLVN+LHQIG KAIWMLDPGIKHEKGY
Sbjct: 294  EKNIPCDVIWMDIDYMDGFRCFTFDPERFTDPKTLVNDLHQIGIKAIWMLDPGIKHEKGY 353

Query: 301  FVYDSGSEKDIWVQKADGDHYVGEVWPGPCVFPDFTQAKARSWWANLVKDFVSNGVDGIW 360
            FVYDSGSEK++WVQKA+G+ YVGEVWPGPCVFP+FTQAKAR WWANLVKDFVSNGVDGIW
Sbjct: 354  FVYDSGSEKNVWVQKANGEPYVGEVWPGPCVFPEFTQAKARDWWANLVKDFVSNGVDGIW 413

Query: 361  NDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMAKSTYEGMKLAKSDR 420
            NDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMA+STYEGMK A S+R
Sbjct: 414  NDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMARSTYEGMKSANSER 473

Query: 421  RPFVLTRAGFIGSQRYAATWTGDNLSSWDHLHMSISMVLQLGLSGQPLSGPDIGGFAGNA 480
            RPFVLTRAGF+GSQ+YAATWTGDN+SSW+HLHMSISM+LQLGLSGQPLSGPDIGGFAGNA
Sbjct: 474  RPFVLTRAGFVGSQKYAATWTGDNISSWEHLHMSISMILQLGLSGQPLSGPDIGGFAGNA 533

Query: 481  TPKLFGRWMGIGALFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF 540
            TP+LFGRWMGIGA+FPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF
Sbjct: 534  TPRLFGRWMGIGAMFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF 593

Query: 541  YMAHTTGVPVATPVFFADSRDPKLRKIENSFLLGSILIYSSTLPNQGMHNLNFTLPKGVW 600
            YMAHTTGVPVATPVFFAD +D  LRKIENSFLLGSILIYSSTLPNQG++NLN TLPKG+W
Sbjct: 594  YMAHTTGVPVATPVFFADPKDANLRKIENSFLLGSILIYSSTLPNQGINNLNLTLPKGIW 653

Query: 601  LRFDFDDSHPDLPALFLQGGSIVPLGPPHQHTGEANPSDDISLLVALDENGKAKGVLFED 660
             RFDFDDSHPDLP LFLQGGSIVPLGP HQHTGEANPSDD+SLLVALDENGKAKGVL+ED
Sbjct: 654  SRFDFDDSHPDLPVLFLQGGSIVPLGPAHQHTGEANPSDDVSLLVALDENGKAKGVLYED 713

Query: 661  DGDGYGFSLGAYLLTHYVAELESSVVTVQVSRTEGSWTRPKRHLHVQILLGGGAKIEAWG 720
            DGDGYGF+LGAYLLTHYVAELESSVVTVQVSRTEGSWTRPKR LHVQILLGGGAKI+AWG
Sbjct: 714  DGDGYGFTLGAYLLTHYVAELESSVVTVQVSRTEGSWTRPKRRLHVQILLGGGAKIDAWG 773

Query: 721  IDGDVLQVTLPSEQQVADLVAISEKEYHHRLEIAKTLPDVEDVPEHKGVALSKIPVELKG 780
             DG+VLQVTLPSEQ+ ADL+AISE+EYHHRLE AKTL DVE V EHKGV+LSK P+ELKG
Sbjct: 774  TDGEVLQVTLPSEQEAADLIAISEREYHHRLERAKTLQDVEGVSEHKGVSLSKTPIELKG 833

Query: 781  AHWSVKVIPWIGGRIVSMTHLPSGIQWLQSKIDINGYEEFSGTEYLSAGCTEEYSIIDRN 840
             HWSVKV+PWIGGRI+SMTHLPSGIQWLQ KIDINGYEEFSGTEY SAGCTEEYSIIDR 
Sbjct: 834  GHWSVKVVPWIGGRIISMTHLPSGIQWLQGKIDINGYEEFSGTEYRSAGCTEEYSIIDRK 893

Query: 841  FEHAGDEESLELEGDIDGGLALRRKIYIPKDYARVLQINSSIIAVKVGAGSGGFSRLVCL 900
            FE  GDEESLELEGDIDGGLALRRKIYI K+ A+ LQINSSI+AVKVGAGSGGFSRLVCL
Sbjct: 894  FELDGDEESLELEGDIDGGLALRRKIYISKEDAKALQINSSIVAVKVGAGSGGFSRLVCL 953

Query: 901  RVHPMFNLLHPTESFISFTSINGSVHEIWPESGEQFFEGDLLPNGEWKLVDKCLGLALVN 960
            RVHPMFNLLHP+ESFISFTS++GSVHEIWPESGEQ+FEGDLLPNGEWKLVDKCLGLALVN
Sbjct: 954  RVHPMFNLLHPSESFISFTSVDGSVHEIWPESGEQYFEGDLLPNGEWKLVDKCLGLALVN 1013

Query: 961  KFNIKEVYKSFIHWGTGTVNLELWSEQRPVSKQTPIRISHSYEVI 1005
            KFN+KEVYK F++WGTGTVNLELWSEQRPVSK+TP+RI HSYEV+
Sbjct: 1014 KFNVKEVYKCFVYWGTGTVNLELWSEQRPVSKETPLRILHSYEVM 1058

BLAST of MC10g1385 vs. ExPASy TrEMBL
Match: A0A6J1HQT7 (uncharacterized protein LOC111465248 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111465248 PE=3 SV=1)

HSP 1 Score: 1917 bits (4965), Expect = 0.0
Identity = 899/1005 (89.45%), Postives = 963/1005 (95.82%), Query Frame = 0

Query: 1    RRKKINQKLISGSFTCKMADGKERGITTDTISGNMIFEPILEDGVFRFDCSSHDRAAAYP 60
            RRK  N+KLISG FTCKM + KE+GIT DTISGNMIFEPILEDGVFRFDCS++DRAAAYP
Sbjct: 54   RRKWTNKKLISGKFTCKMGNTKEKGITIDTISGNMIFEPILEDGVFRFDCSANDRAAAYP 113

Query: 61   SFSFLRSEDRDTPISSQKLPTYVPVFECLLGQQIVKLELPAGTSFYGTGEVSGQLERTGK 120
            SFSF++S+DRDTPISSQKL TYVPVFEC LGQQIVKL+LPAGTSFYGTGEVSGQLERTGK
Sbjct: 114  SFSFIKSKDRDTPISSQKLATYVPVFECHLGQQIVKLQLPAGTSFYGTGEVSGQLERTGK 173

Query: 121  RIFTWNTDAYGYGAGTTSLYQAHPWVLAILPNGQALGVLADTSLRCEIDLRKDSIIQFVA 180
            RIFTWNTDAYGYG+GTTSLYQ+HPWVLAILPNG+ALG+LADT LRCEIDLR+DSIIQF+A
Sbjct: 174  RIFTWNTDAYGYGSGTTSLYQSHPWVLAILPNGEALGILADTFLRCEIDLREDSIIQFIA 233

Query: 181  PSSYPVITFGPFSSPTAVLKAFSSAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR 240
            PSSYPVITFGPFSSP+AVLK+FS AVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR
Sbjct: 234  PSSYPVITFGPFSSPSAVLKSFSKAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR 293

Query: 241  EKDIPCDVIWMDIDYMDGFRCFTFDPERFADPKTLVNELHQIGFKAIWMLDPGIKHEKGY 300
            EK+IPCDVIWMDIDYMDGFRCFTFDPERF DPKTLVN+LHQIG KAIWMLDPGIKHEKGY
Sbjct: 294  EKNIPCDVIWMDIDYMDGFRCFTFDPERFTDPKTLVNDLHQIGIKAIWMLDPGIKHEKGY 353

Query: 301  FVYDSGSEKDIWVQKADGDHYVGEVWPGPCVFPDFTQAKARSWWANLVKDFVSNGVDGIW 360
            FVYDSGSEK++WVQKA+G+ YVGEVWPGPCVFP+FTQAKAR WWANLVKDFVSNGVDGIW
Sbjct: 354  FVYDSGSEKNVWVQKANGEPYVGEVWPGPCVFPEFTQAKARDWWANLVKDFVSNGVDGIW 413

Query: 361  NDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMAKSTYEGMKLAKSDR 420
            NDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMA+STYEGMK A S+R
Sbjct: 414  NDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMARSTYEGMKSANSER 473

Query: 421  RPFVLTRAGFIGSQRYAATWTGDNLSSWDHLHMSISMVLQLGLSGQPLSGPDIGGFAGNA 480
            RPFVLTRAGF+GSQ+YAATWTGDN+SSW+HLHMSISM+LQLGLSGQPLSGPDIGGFAGNA
Sbjct: 474  RPFVLTRAGFVGSQKYAATWTGDNISSWEHLHMSISMILQLGLSGQPLSGPDIGGFAGNA 533

Query: 481  TPKLFGRWMGIGALFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF 540
            TP+LFGRWMGIGA+FPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF
Sbjct: 534  TPRLFGRWMGIGAMFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF 593

Query: 541  YMAHTTGVPVATPVFFADSRDPKLRKIENSFLLGSILIYSSTLPNQGMHNLNFTLPKGVW 600
            YMAHTTGVPVATPVFFAD +D  LRKIENSFLLGSILIYSSTLPNQG++NLN TLPKG+W
Sbjct: 594  YMAHTTGVPVATPVFFADPKDANLRKIENSFLLGSILIYSSTLPNQGINNLNLTLPKGIW 653

Query: 601  LRFDFDDSHPDLPALFLQGGSIVPLGPPHQHTGEANPSDDISLLVALDENGKAKGVLFED 660
             RFDFDDSHPDLP LFLQGGSIVPLGP HQHTGEANPSDD+SLLVALDENGKAKGVL+ED
Sbjct: 654  SRFDFDDSHPDLPVLFLQGGSIVPLGPAHQHTGEANPSDDVSLLVALDENGKAKGVLYED 713

Query: 661  DGDGYGFSLGAYLLTHYVAELESSVVTVQVSRTEGSWTRPKRHLHVQILLGGGAKIEAWG 720
            DGDGYGF+LGAYLLTHYVAELESSVVTVQVSRTEGSWTRPKR LHVQILLGGGAKI+AWG
Sbjct: 714  DGDGYGFTLGAYLLTHYVAELESSVVTVQVSRTEGSWTRPKRRLHVQILLGGGAKIDAWG 773

Query: 721  IDGDVLQVTLPSEQQVADLVAISEKEYHHRLEIAKTLPDVEDVPEHKGVALSKIPVELKG 780
             DG+VLQVTLPSEQ+ ADL+AISE+EYHHRLE AKTL DVE V EHKGV+LSK P+ELKG
Sbjct: 774  TDGEVLQVTLPSEQEAADLIAISEREYHHRLERAKTLQDVEGVSEHKGVSLSKTPIELKG 833

Query: 781  AHWSVKVIPWIGGRIVSMTHLPSGIQWLQSKIDINGYEEFSGTEYLSAGCTEEYSIIDRN 840
             HWSVKV+PWIGGRI+SMTHLPSGIQWLQ KIDINGYEEFSGTEY SAGCTEEYSII++ 
Sbjct: 834  GHWSVKVVPWIGGRIISMTHLPSGIQWLQGKIDINGYEEFSGTEYRSAGCTEEYSIIEK- 893

Query: 841  FEHAGDEESLELEGDIDGGLALRRKIYIPKDYARVLQINSSIIAVKVGAGSGGFSRLVCL 900
            FE  GDEESLELEGDIDGGLALRRKIYI K+ A+ LQINSSI+AVKVGAGSGGFSRLVCL
Sbjct: 894  FELDGDEESLELEGDIDGGLALRRKIYISKEDAKALQINSSIVAVKVGAGSGGFSRLVCL 953

Query: 901  RVHPMFNLLHPTESFISFTSINGSVHEIWPESGEQFFEGDLLPNGEWKLVDKCLGLALVN 960
            RVHPMFNLLHP+ESFISFTS++GSVHEIWPESGEQ+FEGDLLPNGEWKLVDKCLGLALVN
Sbjct: 954  RVHPMFNLLHPSESFISFTSVDGSVHEIWPESGEQYFEGDLLPNGEWKLVDKCLGLALVN 1013

Query: 961  KFNIKEVYKSFIHWGTGTVNLELWSEQRPVSKQTPIRISHSYEVI 1005
            KFN+KEVYK F++WGTGTVNLELWSEQRPVSK+TP+RI HSYEV+
Sbjct: 1014 KFNVKEVYKCFVYWGTGTVNLELWSEQRPVSKETPLRILHSYEVM 1057

BLAST of MC10g1385 vs. ExPASy TrEMBL
Match: A0A6J1E5M6 (uncharacterized protein LOC111430993 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430993 PE=3 SV=1)

HSP 1 Score: 1911 bits (4951), Expect = 0.0
Identity = 894/1005 (88.96%), Postives = 957/1005 (95.22%), Query Frame = 0

Query: 1    RRKKINQKLISGSFTCKMADGKERGITTDTISGNMIFEPILEDGVFRFDCSSHDRAAAYP 60
            RRK  N+KLISG F CKM + KE+GIT DT+SGNMIFEPILEDGVFRFDCS++DRAAAYP
Sbjct: 54   RRKWTNKKLISGKFACKMGNTKEKGITIDTVSGNMIFEPILEDGVFRFDCSANDRAAAYP 113

Query: 61   SFSFLRSEDRDTPISSQKLPTYVPVFECLLGQQIVKLELPAGTSFYGTGEVSGQLERTGK 120
            SFSF++S+DRDTPISSQKL TYVPVFEC LGQQIVKL+LPAGTSFYGTGEVSGQLERTGK
Sbjct: 114  SFSFIKSKDRDTPISSQKLATYVPVFECHLGQQIVKLQLPAGTSFYGTGEVSGQLERTGK 173

Query: 121  RIFTWNTDAYGYGAGTTSLYQAHPWVLAILPNGQALGVLADTSLRCEIDLRKDSIIQFVA 180
            RIFTWNTDAYGYG+GTTSLYQ+HPWVLAILPNG+ALGVLADTSLRCEIDLR+DSIIQF+A
Sbjct: 174  RIFTWNTDAYGYGSGTTSLYQSHPWVLAILPNGEALGVLADTSLRCEIDLREDSIIQFIA 233

Query: 181  PSSYPVITFGPFSSPTAVLKAFSSAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR 240
            PSSYPVITFGPFSSP+AVLK+FS AVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR
Sbjct: 234  PSSYPVITFGPFSSPSAVLKSFSKAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR 293

Query: 241  EKDIPCDVIWMDIDYMDGFRCFTFDPERFADPKTLVNELHQIGFKAIWMLDPGIKHEKGY 300
            EK+IPCDVIWMDIDYMDGFRCFTFDPERFADPKTLVN+LHQIG KAIWMLDPGIKHEKGY
Sbjct: 294  EKNIPCDVIWMDIDYMDGFRCFTFDPERFADPKTLVNDLHQIGMKAIWMLDPGIKHEKGY 353

Query: 301  FVYDSGSEKDIWVQKADGDHYVGEVWPGPCVFPDFTQAKARSWWANLVKDFVSNGVDGIW 360
            FVYD+GSEK++WVQKA+G+ YVGEVWPGPCVFP+FTQAKAR WWANLVKDFVSNGVDGIW
Sbjct: 354  FVYDNGSEKNVWVQKANGEPYVGEVWPGPCVFPEFTQAKARDWWANLVKDFVSNGVDGIW 413

Query: 361  NDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMAKSTYEGMKLAKSDR 420
            NDMNEPAIFK VTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMA+STYEGMK A S+R
Sbjct: 414  NDMNEPAIFKTVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMARSTYEGMKSANSER 473

Query: 421  RPFVLTRAGFIGSQRYAATWTGDNLSSWDHLHMSISMVLQLGLSGQPLSGPDIGGFAGNA 480
            RPFVLTRAGF+GSQ+YAATWTGDN+SSW+HLHMSISM+LQLGLSGQPLSGPDIGGFAGNA
Sbjct: 474  RPFVLTRAGFVGSQKYAATWTGDNISSWEHLHMSISMILQLGLSGQPLSGPDIGGFAGNA 533

Query: 481  TPKLFGRWMGIGALFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF 540
            TP+LFGRWMGIGA+FPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF
Sbjct: 534  TPRLFGRWMGIGAMFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF 593

Query: 541  YMAHTTGVPVATPVFFADSRDPKLRKIENSFLLGSILIYSSTLPNQGMHNLNFTLPKGVW 600
            YMAHTTGVPVATP FFAD +D  LRKIENSFLLGSILIYSSTLPNQG+ NLN TLPKG+W
Sbjct: 594  YMAHTTGVPVATPFFFADPKDANLRKIENSFLLGSILIYSSTLPNQGIDNLNLTLPKGIW 653

Query: 601  LRFDFDDSHPDLPALFLQGGSIVPLGPPHQHTGEANPSDDISLLVALDENGKAKGVLFED 660
             RFDFDDSHPDLP LFLQGGSIVPLGP HQHTGEANPSDD+SLLVALDENGKAKGVL+ED
Sbjct: 654  SRFDFDDSHPDLPVLFLQGGSIVPLGPAHQHTGEANPSDDVSLLVALDENGKAKGVLYED 713

Query: 661  DGDGYGFSLGAYLLTHYVAELESSVVTVQVSRTEGSWTRPKRHLHVQILLGGGAKIEAWG 720
            DGDGYGF+LGAYLLTHYVAELESSVV+VQVSRTEGSW RPKR LHVQILLGGGAKI+AWG
Sbjct: 714  DGDGYGFTLGAYLLTHYVAELESSVVSVQVSRTEGSWARPKRRLHVQILLGGGAKIDAWG 773

Query: 721  IDGDVLQVTLPSEQQVADLVAISEKEYHHRLEIAKTLPDVEDVPEHKGVALSKIPVELKG 780
             DG+VLQV LPSEQ+ ADL+AISE+EY HRLE AKTL D E V EHKGV+LSK P+ELKG
Sbjct: 774  TDGEVLQVMLPSEQEAADLIAISEREYRHRLESAKTLQDHEGVSEHKGVSLSKTPIELKG 833

Query: 781  AHWSVKVIPWIGGRIVSMTHLPSGIQWLQSKIDINGYEEFSGTEYLSAGCTEEYSIIDRN 840
             HWSVKV+PWIGGRI+SMTHLPSGIQWLQ KIDINGYEEFSGTEY SAGCTEEYSIIDR 
Sbjct: 834  GHWSVKVVPWIGGRIISMTHLPSGIQWLQGKIDINGYEEFSGTEYRSAGCTEEYSIIDRK 893

Query: 841  FEHAGDEESLELEGDIDGGLALRRKIYIPKDYARVLQINSSIIAVKVGAGSGGFSRLVCL 900
             EH  DEESLELEGDIDGGLALRRKIYI K+ A+ LQINSSI+AVKVGAGSGGFSRLVCL
Sbjct: 894  IEHDEDEESLELEGDIDGGLALRRKIYISKEDAKALQINSSIVAVKVGAGSGGFSRLVCL 953

Query: 901  RVHPMFNLLHPTESFISFTSINGSVHEIWPESGEQFFEGDLLPNGEWKLVDKCLGLALVN 960
            RVHPMFNLLHP+ESFISFTS++GSVHEIWPESGEQ+FEGDLLPNGEWKLVDKCLGLALVN
Sbjct: 954  RVHPMFNLLHPSESFISFTSVDGSVHEIWPESGEQYFEGDLLPNGEWKLVDKCLGLALVN 1013

Query: 961  KFNIKEVYKSFIHWGTGTVNLELWSEQRPVSKQTPIRISHSYEVI 1005
            KFN+KEVYK F+HWGTGTVNLELWSEQRPVSK+TP++ISHSYEV+
Sbjct: 1014 KFNVKEVYKCFVHWGTGTVNLELWSEQRPVSKETPLQISHSYEVM 1058

BLAST of MC10g1385 vs. ExPASy TrEMBL
Match: A0A6J1E909 (uncharacterized protein LOC111430993 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430993 PE=3 SV=1)

HSP 1 Score: 1904 bits (4932), Expect = 0.0
Identity = 892/1005 (88.76%), Postives = 957/1005 (95.22%), Query Frame = 0

Query: 1    RRKKINQKLISGSFTCKMADGKERGITTDTISGNMIFEPILEDGVFRFDCSSHDRAAAYP 60
            RRK  N+KLISG F CKM + KE+GIT DT+SGNMIFEPILEDGVFRFDCS++DRAAAYP
Sbjct: 54   RRKWTNKKLISGKFACKMGNTKEKGITIDTVSGNMIFEPILEDGVFRFDCSANDRAAAYP 113

Query: 61   SFSFLRSEDRDTPISSQKLPTYVPVFECLLGQQIVKLELPAGTSFYGTGEVSGQLERTGK 120
            SFSF++S+DRDTPISSQKL TYVPVFEC LGQQIVKL+LPAGTSFYGTGEVSGQLERTGK
Sbjct: 114  SFSFIKSKDRDTPISSQKLATYVPVFECHLGQQIVKLQLPAGTSFYGTGEVSGQLERTGK 173

Query: 121  RIFTWNTDAYGYGAGTTSLYQAHPWVLAILPNGQALGVLADTSLRCEIDLRKDSIIQFVA 180
            RIFTWNTDAYGYG+GTTSLYQ+HPWVLAILPNG+ALGVLADTSLRCEIDLR+DSIIQF+A
Sbjct: 174  RIFTWNTDAYGYGSGTTSLYQSHPWVLAILPNGEALGVLADTSLRCEIDLREDSIIQFIA 233

Query: 181  PSSYPVITFGPFSSPTAVLKAFSSAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR 240
            PSSYPVITFGPFSSP+AVLK+FS AVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR
Sbjct: 234  PSSYPVITFGPFSSPSAVLKSFSKAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFR 293

Query: 241  EKDIPCDVIWMDIDYMDGFRCFTFDPERFADPKTLVNELHQIGFKAIWMLDPGIKHEKGY 300
            EK+IPCDVIWMDIDYMDGFRCFTFDPERFADPKTLVN+LHQIG KAIWMLDPGIKHEKGY
Sbjct: 294  EKNIPCDVIWMDIDYMDGFRCFTFDPERFADPKTLVNDLHQIGMKAIWMLDPGIKHEKGY 353

Query: 301  FVYDSGSEKDIWVQKADGDHYVGEVWPGPCVFPDFTQAKARSWWANLVKDFVSNGVDGIW 360
            FVYD+GSEK++WVQKA+G+ YVGEVWPGPCVFP+FTQAKAR WWANLVKDFVSNGVDGIW
Sbjct: 354  FVYDNGSEKNVWVQKANGEPYVGEVWPGPCVFPEFTQAKARDWWANLVKDFVSNGVDGIW 413

Query: 361  NDMNEPAIFKAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMAKSTYEGMKLAKSDR 420
            NDMNEPAIFK VTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMA+STYEGMK A S+R
Sbjct: 414  NDMNEPAIFKTVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMARSTYEGMKSANSER 473

Query: 421  RPFVLTRAGFIGSQRYAATWTGDNLSSWDHLHMSISMVLQLGLSGQPLSGPDIGGFAGNA 480
            RPFVLTRAGF+GSQ+YAATWTGDN+SSW+HLHMSISM+LQLGLSGQPLSGPDIGGFAGNA
Sbjct: 474  RPFVLTRAGFVGSQKYAATWTGDNISSWEHLHMSISMILQLGLSGQPLSGPDIGGFAGNA 533

Query: 481  TPKLFGRWMGIGALFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF 540
            TP+LFGRWMGIGA+FPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF
Sbjct: 534  TPRLFGRWMGIGAMFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLF 593

Query: 541  YMAHTTGVPVATPVFFADSRDPKLRKIENSFLLGSILIYSSTLPNQGMHNLNFTLPKGVW 600
            YMAHTTGVPVATP FFAD +D  LRKIENSFLLGSILIYSSTLPNQG+ NLN TLPKG+W
Sbjct: 594  YMAHTTGVPVATPFFFADPKDANLRKIENSFLLGSILIYSSTLPNQGIDNLNLTLPKGIW 653

Query: 601  LRFDFDDSHPDLPALFLQGGSIVPLGPPHQHTGEANPSDDISLLVALDENGKAKGVLFED 660
             RFDFDDSHPDLP LFLQGGSIVPLGP HQHTGEANPSDD+SLLVALDENGKAKGVL+ED
Sbjct: 654  SRFDFDDSHPDLPVLFLQGGSIVPLGPAHQHTGEANPSDDVSLLVALDENGKAKGVLYED 713

Query: 661  DGDGYGFSLGAYLLTHYVAELESSVVTVQVSRTEGSWTRPKRHLHVQILLGGGAKIEAWG 720
            DGDGYGF+LGAYLLTHYVAELESSVV+VQVSRTEGSW RPKR LHVQILLGGGAKI+AWG
Sbjct: 714  DGDGYGFTLGAYLLTHYVAELESSVVSVQVSRTEGSWARPKRRLHVQILLGGGAKIDAWG 773

Query: 721  IDGDVLQVTLPSEQQVADLVAISEKEYHHRLEIAKTLPDVEDVPEHKGVALSKIPVELKG 780
             DG+VLQV LPSEQ+ ADL+AISE+EY HRLE AKTL D E V EHKGV+LSK P+ELKG
Sbjct: 774  TDGEVLQVMLPSEQEAADLIAISEREYRHRLESAKTLQDHEGVSEHKGVSLSKTPIELKG 833

Query: 781  AHWSVKVIPWIGGRIVSMTHLPSGIQWLQSKIDINGYEEFSGTEYLSAGCTEEYSIIDRN 840
             HWSVKV+PWIGGRI+SMTHLPSGIQWLQ KIDINGYEEFSGTEY SAGCTEEYSII++ 
Sbjct: 834  GHWSVKVVPWIGGRIISMTHLPSGIQWLQGKIDINGYEEFSGTEYRSAGCTEEYSIIEK- 893

Query: 841  FEHAGDEESLELEGDIDGGLALRRKIYIPKDYARVLQINSSIIAVKVGAGSGGFSRLVCL 900
             EH  DEESLELEGDIDGGLALRRKIYI K+ A+ LQINSSI+AVKVGAGSGGFSRLVCL
Sbjct: 894  IEHDEDEESLELEGDIDGGLALRRKIYISKEDAKALQINSSIVAVKVGAGSGGFSRLVCL 953

Query: 901  RVHPMFNLLHPTESFISFTSINGSVHEIWPESGEQFFEGDLLPNGEWKLVDKCLGLALVN 960
            RVHPMFNLLHP+ESFISFTS++GSVHEIWPESGEQ+FEGDLLPNGEWKLVDKCLGLALVN
Sbjct: 954  RVHPMFNLLHPSESFISFTSVDGSVHEIWPESGEQYFEGDLLPNGEWKLVDKCLGLALVN 1013

Query: 961  KFNIKEVYKSFIHWGTGTVNLELWSEQRPVSKQTPIRISHSYEVI 1005
            KFN+KEVYK F+HWGTGTVNLELWSEQRPVSK+TP++ISHSYEV+
Sbjct: 1014 KFNVKEVYKCFVHWGTGTVNLELWSEQRPVSKETPLQISHSYEVM 1057

BLAST of MC10g1385 vs. TAIR 10
Match: AT3G23640.1 (heteroglycan glucosidase 1 )

HSP 1 Score: 1539.6 bits (3985), Expect = 0.0e+00
Identity = 688/978 (70.35%), Postives = 822/978 (84.05%), Query Frame = 0

Query: 27   TTDTISGNMIFEPILEDGVFRFDCSSHDRAAAYPSFSFLRSEDRDTPISSQKLPTYVPVF 86
            T +  S +MIFEPILE GVFRFDCS   R AA+PS SF  S+DR+ PI S  +P Y+P  
Sbjct: 10   TVEMTSTDMIFEPILEHGVFRFDCSVDHRKAAFPSVSFKNSKDREVPIVSHIVPAYIPTC 69

Query: 87   ECLLGQQIVKLELPAGTSFYGTGEVSGQLERTGKRIFTWNTDAYGYGAGTTSLYQAHPWV 146
             CL  QQ+V  E   GTSFYGTGEVSGQLERTGKR+FTWNTDA+GYG+GTTSLYQ+HPWV
Sbjct: 70   GCLQDQQVVTFEFSPGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGSGTTSLYQSHPWV 129

Query: 147  LAILPNGQALGVLADTSLRCEIDLRKDSIIQFVAPSSYPVITFGPFSSPTAVLKAFSSAV 206
            L +LP G+ LGVLADT+ +CEIDLRK+ II+ ++P+SYP+ITFGPFSSPTAVL++ S A+
Sbjct: 130  LVVLPTGETLGVLADTTRKCEIDLRKEGIIRIISPASYPIITFGPFSSPTAVLESLSHAI 189

Query: 207  GTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFREKDIPCDVIWMDIDYMDGFRCFTFDP 266
            GTVFMPPKWALGYHQCRWSY S  RV E+++TFR+K IP DVIWMDIDYMDGFRCFTFD 
Sbjct: 190  GTVFMPPKWALGYHQCRWSYMSDKRVAEIAQTFRDKKIPSDVIWMDIDYMDGFRCFTFDK 249

Query: 267  ERFADPKTLVNELHQIGFKAIWMLDPGIKHEKGYFVYDSGSEKDIWVQKADGDHYVGEVW 326
            ERF DP  L  +LH  GFKAIWMLDPGIK E+GY+VYDSGS+ D+W+ +ADG  + GEVW
Sbjct: 250  ERFPDPSALAKDLHSNGFKAIWMLDPGIKQEEGYYVYDSGSKNDVWISRADGKPFTGEVW 309

Query: 327  PGPCVFPDFTQAKARSWWANLVKDFVSNGVDGIWNDMNEPAIFKAVTKTMPESNIHRGDE 386
            PGPCVFPD+T +KARSWWANLVK+FVSNGVDGIWNDMNEPA+FK VTKTMPE+NIH GD+
Sbjct: 310  PGPCVFPDYTNSKARSWWANLVKEFVSNGVDGIWNDMNEPAVFKVVTKTMPENNIHHGDD 369

Query: 387  EFGGCQNHSYYHNVYGMLMAKSTYEGMKLAKSDRRPFVLTRAGFIGSQRYAATWTGDNLS 446
            E GG QNHS+YHNVYGMLMA+STYEGM+LA  ++RPFVLTRAGFIGSQRYAATWTGDNLS
Sbjct: 370  ELGGVQNHSHYHNVYGMLMARSTYEGMELADKNKRPFVLTRAGFIGSQRYAATWTGDNLS 429

Query: 447  SWDHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPKLFGRWMGIGALFPFCRGHSETST 506
            +W+HLHMSISMVLQLGLSGQPLSGPDIGGFAGNATP+LFGRWMG+GA+FPFCRGHSE  T
Sbjct: 430  NWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRLFGRWMGVGAMFPFCRGHSEAGT 489

Query: 507  ADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLFYMAHTTGVPVATPVFFADSRDPKLRK 566
             DHEPWSFGEECEEVCR ALKRRY+LLPH YTLFY+AHTTG PVA P+FFAD  D +LR 
Sbjct: 490  DDHEPWSFGEECEEVCRAALKRRYQLLPHFYTLFYIAHTTGAPVAAPIFFADPIDSRLRA 549

Query: 567  IENSFLLGSILIYSSTLPNQGMHNLNFTLPKGVWLRFDFDDSHPDLPALFLQGGSIVPLG 626
            +EN FLLG +LIY+STL +QG H L   LP+G+W RFDF DSHPDLP L+LQGGSI+ L 
Sbjct: 550  VENGFLLGPLLIYASTLSSQGSHELQHILPRGIWHRFDFADSHPDLPTLYLQGGSIISLA 609

Query: 627  PPHQHTGEANPSDDISLLVALDENGKAKGVLFEDDGDGYGFSLGAYLLTHYVAELESSVV 686
            PPH H GE + SDD++LLV+LDENGKAKG+LFEDDGDGYG++ G +L+THY+AE +SS V
Sbjct: 610  PPHLHVGEFSLSDDLTLLVSLDENGKAKGLLFEDDGDGYGYTKGRFLVTHYIAERDSSTV 669

Query: 687  TVQVSRTEGSWTRPKRHLHVQILLGGGAKIEAWGIDGDVLQVTLPSEQQVADLVAISEKE 746
            TV+VS+TEG W RP R +HVQ+LLGGGA ++AWG+DG+ + + +PSE  +++L++ S + 
Sbjct: 670  TVKVSKTEGDWQRPNRRVHVQLLLGGGAMLDAWGMDGEFIHIKVPSESGISELISTSNER 729

Query: 747  YHHRLEIAKTLPDVEDVPEHKGVALSKIPVELKGAHWSVKVIPWIGGRIVSMTHLPSGIQ 806
            +   +E  K +P+ E VP  KG+ LSK PVEL    W + ++PW+GGRI+SMTH+PSGIQ
Sbjct: 730  FKLHMENTKLIPEKEVVPGQKGMELSKEPVELSSGDWKLNIVPWVGGRILSMTHVPSGIQ 789

Query: 807  WLQSKIDINGYEEFSGTEYLSAGCTEEYSIIDRNFEHAGDEESLELEGDIDGGLALRRKI 866
            WL S+IDINGYEE+SGTEY SAGCTEEY++I+R+ EHAG+EESL LEGD+ GGL LRRKI
Sbjct: 790  WLHSRIDINGYEEYSGTEYRSAGCTEEYNVIERDLEHAGEEESLILEGDVGGGLVLRRKI 849

Query: 867  YIPKDYARVLQINSSIIAVKVGAGSGGFSRLVCLRVHPMFNLLHPTESFISFTSINGSVH 926
             I KD  RV +I SSI A  VGAGSGGFSRLVCLRVHP F LLHPTESF+SFTSI+GS H
Sbjct: 850  SIAKDNQRVFRIASSIEARSVGAGSGGFSRLVCLRVHPTFTLLHPTESFVSFTSIDGSKH 909

Query: 927  EIWPESGEQFFEGDLLPNGEWKLVDKCLGLALVNKFNIKEVYKSFIHWGTGTVNLELWSE 986
            E+WP+SG+Q +EG+ LP+G+W LVDK L L +VN+F++ +V+K  IHW  GTVNLELWS+
Sbjct: 910  EVWPDSGDQIYEGNNLPHGKWMLVDKSLNLRMVNRFDVSQVFKCIIHWDCGTVNLELWSK 969

Query: 987  QRPVSKQTPIRISHSYEV 1005
            +RPVSK++P++I H YEV
Sbjct: 970  ERPVSKESPLKIEHEYEV 987

BLAST of MC10g1385 vs. TAIR 10
Match: AT3G23640.2 (heteroglycan glucosidase 1 )

HSP 1 Score: 1539.6 bits (3985), Expect = 0.0e+00
Identity = 688/978 (70.35%), Postives = 822/978 (84.05%), Query Frame = 0

Query: 27   TTDTISGNMIFEPILEDGVFRFDCSSHDRAAAYPSFSFLRSEDRDTPISSQKLPTYVPVF 86
            T +  S +MIFEPILE GVFRFDCS   R AA+PS SF  S+DR+ PI S  +P Y+P  
Sbjct: 10   TVEMTSTDMIFEPILEHGVFRFDCSVDHRKAAFPSVSFKNSKDREVPIVSHIVPAYIPTC 69

Query: 87   ECLLGQQIVKLELPAGTSFYGTGEVSGQLERTGKRIFTWNTDAYGYGAGTTSLYQAHPWV 146
             CL  QQ+V  E   GTSFYGTGEVSGQLERTGKR+FTWNTDA+GYG+GTTSLYQ+HPWV
Sbjct: 70   GCLQDQQVVTFEFSPGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGSGTTSLYQSHPWV 129

Query: 147  LAILPNGQALGVLADTSLRCEIDLRKDSIIQFVAPSSYPVITFGPFSSPTAVLKAFSSAV 206
            L +LP G+ LGVLADT+ +CEIDLRK+ II+ ++P+SYP+ITFGPFSSPTAVL++ S A+
Sbjct: 130  LVVLPTGETLGVLADTTRKCEIDLRKEGIIRIISPASYPIITFGPFSSPTAVLESLSHAI 189

Query: 207  GTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFREKDIPCDVIWMDIDYMDGFRCFTFDP 266
            GTVFMPPKWALGYHQCRWSY S  RV E+++TFR+K IP DVIWMDIDYMDGFRCFTFD 
Sbjct: 190  GTVFMPPKWALGYHQCRWSYMSDKRVAEIAQTFRDKKIPSDVIWMDIDYMDGFRCFTFDK 249

Query: 267  ERFADPKTLVNELHQIGFKAIWMLDPGIKHEKGYFVYDSGSEKDIWVQKADGDHYVGEVW 326
            ERF DP  L  +LH  GFKAIWMLDPGIK E+GY+VYDSGS+ D+W+ +ADG  + GEVW
Sbjct: 250  ERFPDPSALAKDLHSNGFKAIWMLDPGIKQEEGYYVYDSGSKNDVWISRADGKPFTGEVW 309

Query: 327  PGPCVFPDFTQAKARSWWANLVKDFVSNGVDGIWNDMNEPAIFKAVTKTMPESNIHRGDE 386
            PGPCVFPD+T +KARSWWANLVK+FVSNGVDGIWNDMNEPA+FK VTKTMPE+NIH GD+
Sbjct: 310  PGPCVFPDYTNSKARSWWANLVKEFVSNGVDGIWNDMNEPAVFKVVTKTMPENNIHHGDD 369

Query: 387  EFGGCQNHSYYHNVYGMLMAKSTYEGMKLAKSDRRPFVLTRAGFIGSQRYAATWTGDNLS 446
            E GG QNHS+YHNVYGMLMA+STYEGM+LA  ++RPFVLTRAGFIGSQRYAATWTGDNLS
Sbjct: 370  ELGGVQNHSHYHNVYGMLMARSTYEGMELADKNKRPFVLTRAGFIGSQRYAATWTGDNLS 429

Query: 447  SWDHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPKLFGRWMGIGALFPFCRGHSETST 506
            +W+HLHMSISMVLQLGLSGQPLSGPDIGGFAGNATP+LFGRWMG+GA+FPFCRGHSE  T
Sbjct: 430  NWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRLFGRWMGVGAMFPFCRGHSEAGT 489

Query: 507  ADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLFYMAHTTGVPVATPVFFADSRDPKLRK 566
             DHEPWSFGEECEEVCR ALKRRY+LLPH YTLFY+AHTTG PVA P+FFAD  D +LR 
Sbjct: 490  DDHEPWSFGEECEEVCRAALKRRYQLLPHFYTLFYIAHTTGAPVAAPIFFADPIDSRLRA 549

Query: 567  IENSFLLGSILIYSSTLPNQGMHNLNFTLPKGVWLRFDFDDSHPDLPALFLQGGSIVPLG 626
            +EN FLLG +LIY+STL +QG H L   LP+G+W RFDF DSHPDLP L+LQGGSI+ L 
Sbjct: 550  VENGFLLGPLLIYASTLSSQGSHELQHILPRGIWHRFDFADSHPDLPTLYLQGGSIISLA 609

Query: 627  PPHQHTGEANPSDDISLLVALDENGKAKGVLFEDDGDGYGFSLGAYLLTHYVAELESSVV 686
            PPH H GE + SDD++LLV+LDENGKAKG+LFEDDGDGYG++ G +L+THY+AE +SS V
Sbjct: 610  PPHLHVGEFSLSDDLTLLVSLDENGKAKGLLFEDDGDGYGYTKGRFLVTHYIAERDSSTV 669

Query: 687  TVQVSRTEGSWTRPKRHLHVQILLGGGAKIEAWGIDGDVLQVTLPSEQQVADLVAISEKE 746
            TV+VS+TEG W RP R +HVQ+LLGGGA ++AWG+DG+ + + +PSE  +++L++ S + 
Sbjct: 670  TVKVSKTEGDWQRPNRRVHVQLLLGGGAMLDAWGMDGEFIHIKVPSESGISELISTSNER 729

Query: 747  YHHRLEIAKTLPDVEDVPEHKGVALSKIPVELKGAHWSVKVIPWIGGRIVSMTHLPSGIQ 806
            +   +E  K +P+ E VP  KG+ LSK PVEL    W + ++PW+GGRI+SMTH+PSGIQ
Sbjct: 730  FKLHMENTKLIPEKEVVPGQKGMELSKEPVELSSGDWKLNIVPWVGGRILSMTHVPSGIQ 789

Query: 807  WLQSKIDINGYEEFSGTEYLSAGCTEEYSIIDRNFEHAGDEESLELEGDIDGGLALRRKI 866
            WL S+IDINGYEE+SGTEY SAGCTEEY++I+R+ EHAG+EESL LEGD+ GGL LRRKI
Sbjct: 790  WLHSRIDINGYEEYSGTEYRSAGCTEEYNVIERDLEHAGEEESLILEGDVGGGLVLRRKI 849

Query: 867  YIPKDYARVLQINSSIIAVKVGAGSGGFSRLVCLRVHPMFNLLHPTESFISFTSINGSVH 926
             I KD  RV +I SSI A  VGAGSGGFSRLVCLRVHP F LLHPTESF+SFTSI+GS H
Sbjct: 850  SIAKDNQRVFRIASSIEARSVGAGSGGFSRLVCLRVHPTFTLLHPTESFVSFTSIDGSKH 909

Query: 927  EIWPESGEQFFEGDLLPNGEWKLVDKCLGLALVNKFNIKEVYKSFIHWGTGTVNLELWSE 986
            E+WP+SG+Q +EG+ LP+G+W LVDK L L +VN+F++ +V+K  IHW  GTVNLELWS+
Sbjct: 910  EVWPDSGDQIYEGNNLPHGKWMLVDKSLNLRMVNRFDVSQVFKCIIHWDCGTVNLELWSK 969

Query: 987  QRPVSKQTPIRISHSYEV 1005
            +RPVSK++P++I H YEV
Sbjct: 970  ERPVSKESPLKIEHEYEV 987

BLAST of MC10g1385 vs. TAIR 10
Match: AT5G63840.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 352.4 bits (903), Expect = 1.2e-96
Identity = 186/501 (37.13%), Postives = 270/501 (53.89%), Query Frame = 0

Query: 195 PTAVLKAFSSAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFREKDIPCDVIWMDID 254
           P  V+K ++S  GT  MP  +A GYHQCRW+Y   + V +V   F E DIP DV+W+DI+
Sbjct: 342 PKDVVKQYASVTGTSAMPQLFATGYHQCRWNYKDEEDVAQVDSKFDEHDIPYDVLWLDIE 401

Query: 255 YMDGFRCFTFDPERFADPKTLVNELHQIGFKAIWMLDPGIKHEKGYFVYDSGSEKDIWVQ 314
           + DG R FT+D   F  P+ +  +L   G K + ++DP IK +  YF++   ++   +V+
Sbjct: 402 HTDGKRYFTWDSVLFPHPEEMQKKLAAKGRKMVTIVDPHIKRDDSYFLHKEATQMGYYVK 461

Query: 315 KADGDHYVGEVWPGPCVFPDFTQAKARSWWAN--LVKDFVSNGVD-GIWNDMNEPAIFKA 374
            + G  + G  WPG   + D    + R WW      K++V +      WNDMNEP++F  
Sbjct: 462 DSSGKDFDGWCWPGSSSYIDMLSPEIRKWWGGRFSYKNYVGSTPSLYTWNDMNEPSVFNG 521

Query: 375 VTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMAKSTYEGMKLAKSDR-RPFVLTRAGF 434
              TMP   +H G  E      H   HN YG     +T +G+ + +  + RPFVL+RA F
Sbjct: 522 PEVTMPRDALHVGGVE------HREVHNAYGYYFHMATSDGLVMREEGKDRPFVLSRAIF 581

Query: 435 IGSQRYAATWTGDNLSSWDHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPKLFGRWMG 494
            G+QRY A WTGDN + W+HL +SI M+L LGL+G   SG DIGGF GN  P+L  RW  
Sbjct: 582 PGTQRYGAIWTGDNTAEWEHLRVSIPMILTLGLTGITFSGADIGGFFGNPEPELLVRWYQ 641

Query: 495 IGALFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLFYMAHTTGVPV 554
           +GA +PF RGH+   T   EPW FGE   E+ R A+  RY LLP+ YTLF  A+ TGVPV
Sbjct: 642 VGAYYPFFRGHAHHDTKRREPWLFGERNTELMRDAIHTRYTLLPYFYTLFREANVTGVPV 701

Query: 555 ATPVFFADSRDPKLRKIENSFLLGSILIYSSTLPNQGMHNLNFTLP-KGVWL-------- 614
             P++    +D      + +F++GS L+       +G    +  LP K  W         
Sbjct: 702 VRPLWMEFPQDEATFSNDEAFMVGSGLLVQGVY-TKGTTQASVYLPGKESWYDLRNGKTY 761

Query: 615 ----RFDFDDSHPDLPALFLQGGSIVPLGPPHQHTGEANPSDDISLLVALDENGKAKGVL 674
                   D     +PA F + G+I+P     + +     +D  +L+VAL+ + +A+G L
Sbjct: 762 VGGKTHKMDAPEESIPA-FQKAGTIIPRKDRFRRSSSQMDNDPYTLVVALNSSQEAEGEL 821

Query: 675 FEDDGDGYGFSLGAYLLTHYV 679
           + DDG  + F  G+Y+   +V
Sbjct: 822 YIDDGKSFEFRRGSYIHRRFV 834

BLAST of MC10g1385 vs. TAIR 10
Match: AT5G11720.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 322.8 bits (826), Expect = 9.9e-88
Identity = 226/724 (31.22%), Postives = 346/724 (47.79%), Query Frame = 0

Query: 103 TSFYGTGEVSGQLER--TGKRIFTWNTDAYGYGAGTTSLYQAHPWVLAILPN------GQ 162
           ++ YG GE + +  R   G+ +  WN D  G      +LY +HP+ + +  +      G 
Sbjct: 192 SNLYGIGEHTKRSFRLIPGETMTLWNAD-IGSENPDVNLYGSHPFYMDVRGSKGNEEAGT 251

Query: 163 ALGVLADTSLRCEIDLRKDSIIQFVAPSSYPVITF-GPFSSPTAVLKAFSSAVGTVFMPP 222
             GVL   S   ++      I   V      +  F GP  SP  V+  ++  +G     P
Sbjct: 252 THGVLLLNSNGMDVKYEGHRITYNVIGGVIDLYVFAGP--SPEMVMNQYTELIGRPAPMP 311

Query: 223 KWALGYHQCRWSYDSADRVLEVSRTFREKDIPCDVIWMDIDYMDGFRCFTFDPERFADPK 282
            W+ G+HQCR+ Y +   +  V   + +  IP +V+W DIDYMDG++ FT DP  F + K
Sbjct: 312 YWSFGFHQCRYGYKNVSDLEYVVDGYAKAGIPLEVMWTDIDYMDGYKDFTLDPVNFPEDK 371

Query: 283 --TLVNELHQIGFKAIWMLDPGIKHEKGYFVYDSGSEKDIWVQKADGDHYVGEVWPGPCV 342
             + V+ LH+ G K + +LDPGI  +  Y  Y+ G E D+++ K +G+ Y+GEVWPG   
Sbjct: 372 MQSFVDTLHKNGQKYVLILDPGIGVDSSYGTYNRGMEADVFI-KRNGEPYLGEVWPGKVY 431

Query: 343 FPDFTQAKARSWWANLVKDFVS-NGVDGIWNDMNEPAIF--------------------- 402
           FPDF    A ++W+N +K F     +DG+W DMNE + F                     
Sbjct: 432 FPDFLNPAAATFWSNEIKMFQEILPLDGLWIDMNELSNFITSPLSSGSSLDDPPYKINNS 491

Query: 403 ----KAVTKTMPESNIHRGDEEFGGCQNHSYYHNVYGMLMAKSTYEGMKLAKSDRRPFVL 462
                   KT+P ++IH     FG    +   HN+YG+L AK+T++ + +  + +RPF+L
Sbjct: 492 GDKRPINNKTVPATSIH-----FGNISEYD-AHNLYGLLEAKATHQAV-VDITGKRPFIL 551

Query: 463 TRAGFIGSQRYAATWTGDNLSSWDHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPKLF 522
           +R+ F+ S +Y A WTGDN + W+ L  SI  +L  GL G P+ G DI GF+ + T +L 
Sbjct: 552 SRSTFVSSGKYTAHWTGDNAAKWEDLAYSIPGILNFGLFGIPMVGADICGFSHDTTEELC 611

Query: 523 GRWMGIGALFPFCRGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLFYMAHT 582
            RW+ +GA +PF R HS   TA  E + + +      R  L  R RLLPH+YTL Y AH 
Sbjct: 612 RRWIQLGAFYPFARDHSSLGTARQELYLW-DSVASSARKVLGLRMRLLPHLYTLMYEAHV 671

Query: 583 TGVPVATPVFFADSRDPKLRKIENSFLLGSILIYSSTLPNQGMHNLNFTLPKGVWL-RFD 642
           +G P+A P+FF+  +D K  +I++ FL+G  ++ S  L  QG   ++   P G W   F+
Sbjct: 672 SGNPIARPLFFSFPQDTKTYEIDSQFLIGKSIMVSPAL-KQGAVAVDAYFPAGNWFDLFN 731

Query: 643 FD-------------DSHPDLPALFLQGGSIVPLGPPHQHTGEANPSDDISLLVALDENG 702
           +              D+  D   + ++ GSIV +      T +A  +    LLV      
Sbjct: 732 YSFAVGGDSGKHVRLDTPADHVNVHVREGSIVAMQGEALTTRDARKT-PYQLLVVASRLE 791

Query: 703 KAKGVLFEDDGDGYGFSLGAYLLTHYVAELESSVVTVQVSRTEGSWTRPKRHLHVQILLG 762
              G LF DDG+      G       + +    V    V             L  +++  
Sbjct: 792 NISGELFLDDGENLRMGAGGGNRDWTLVKFRCYVTGKSVV------------LRSEVVNP 851

Query: 763 GGAKIEAWGIDGDVLQVTLPSEQQVADL-VAISEKEYHHRLEIAKTLPDVEDVPEHKGVA 775
             A    W I G V  V   + + V    V  SE+    R+ + KT+ D +D P    V 
Sbjct: 852 EYASKMKWSI-GKVTFVGFENVENVKTYEVRTSERLRSPRISLIKTVSDNDD-PRFLSVE 887

BLAST of MC10g1385 vs. TAIR 10
Match: AT3G45940.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 319.3 bits (817), Expect = 1.1e-86
Identity = 204/598 (34.11%), Postives = 294/598 (49.16%), Query Frame = 0

Query: 95  VKLELPAGTSFYGTGEVSGQ-----LERTGKRIFTWNTDAYGYGAGTTSLYQAHPWVL-- 154
           +   LP   S YG GE S       +      +FT +  A+      T LY +HP  +  
Sbjct: 164 ISTSLPKDASLYGFGENSQANGIKLVPNEPYTLFTEDVSAFNL---NTDLYGSHPVYMDL 223

Query: 155 -AILPNGQALGVLADTSLRCEIDLRKDSIIQFVAPSSYPVITF-GPFSSPTAVLKAFSSA 214
             +     A  VL   S   ++  R DS+   V    +    F GP  SP  V+  ++S 
Sbjct: 224 RNVSGKAYAHSVLLLNSHGMDVFYRGDSLTYKVIGGVFDFYFFAGP--SPLNVVDQYTSL 283

Query: 215 VGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFREKDIPCDVIWMDIDYMDGFRCFTFD 274
           +G     P W+LG+HQCRW Y +   V +V   +++  IP DVIW D DYMDG++ FT D
Sbjct: 284 IGRPAPMPYWSLGFHQCRWGYRNVSVVKDVVDNYQKAKIPLDVIWNDADYMDGYKDFTLD 343

Query: 275 PERFADPKTL--VNELHQIGFKAIWMLDPGIKHEKGYFVYDSGSEKDIWVQKADGDHYVG 334
              F   K L  ++ +H++G K + + DPGI     Y VY  G   D+++ K +G  ++ 
Sbjct: 344 LVNFPHAKLLSFLDRIHKMGMKYVVIKDPGIGVNASYGVYQRGMASDVFI-KYEGKPFLA 403

Query: 335 EVWPGPCVFPDFTQAKARSWWANLVKDFVS-NGVDGIWNDMNE--PAIFKAVT--KTMPE 394
           +VWPGP  FPDF   K  SWW + ++ F     +DG+W DMNE      KA    KT+P 
Sbjct: 404 QVWPGPVYFPDFLNPKTVSWWGDEIRRFHELVPIDGLWIDMNEINATGHKASLGFKTIPT 463

Query: 395 SNIHRGDEEFGGCQNHSYYHNVYGMLMAKSTYEGMKLAKSDRRPFVLTRAGFIGSQRYAA 454
           S  H     + G + +   H++YG   A +T++ + LA   +RPF+L+R+ F+GS +YAA
Sbjct: 464 SAYH-----YNGVREYD-AHSIYGFSEAIATHKAL-LAVQGKRPFILSRSTFVGSGQYAA 523

Query: 455 TWTGDNLSSWDHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPKLFGRWMGIGALFPFC 514
            WTGDN  +W  L +SIS +L  G+ G P+ G DI GF      +L  RW+ +GA +PF 
Sbjct: 524 HWTGDNQGTWQSLQVSISTMLNFGIFGVPMVGSDICGFFPPTPEELCNRWIEVGAFYPFS 583

Query: 515 RGHSETSTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLFYMAHTTGVPVATPVFFAD 574
           R H++      E + +G   E   R AL  RY+LLP +YTL Y AH +G P+A P+FF+ 
Sbjct: 584 RDHADYYAPRKELYQWGTVAES-ARNALGMRYKLLPFLYTLNYEAHMSGAPIARPLFFSF 643

Query: 575 SRDPKLRKIENSFLLGSILIYSSTLPNQGMHNLNFTLPKGVWLRFDFDDSHPDLPALFLQ 634
               +   +   FLLGS L+ S  L  QG   +    P G W    FD +      +  +
Sbjct: 644 PEFTECYGLSKQFLLGSSLMISPVL-EQGKTQVEALFPPGSWYHM-FDMTQ----VVVSK 703

Query: 635 GGSIVPLGPP------HQHTGEANPSDDISLLVALDENGKAKGVLFEDDGDGYGFSLG 671
            G +  L  P      H +     P   +    A    G A G LF DD +     LG
Sbjct: 704 NGRLFTLPAPFNVVNVHLYQNAILPMQQVVAFPAGASEGYASGKLFLDDDELPEMKLG 741