MC10g1384 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g1384
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionurea-proton symporter DUR3
LocationMC10: 17267099 .. 17271775 (-)
RNA-Seq ExpressionMC10g1384
SyntenyMC10g1384
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
TGCCATTAAGATTGTGAAATCAAACTTGACTCGCTGACTCGGTAATAATTACCACGTATCTCAAACTAAACTAAGAGCCGGCTCTGAGTTGGATCATTTAAAAAGGGACTTCCGAATAAACGAAGAAAGATATTTCAAAATCTCAATAATACATTGTCCAAAGACTTTTCTCACTTCTTCTTCTTGTTCTTCAAAGTTGTGGTGTGTAGCAGCAGCAGAGATTGTGAAGCTTTTGCGGTGGTTTCATCAGCCATGGCTGCTCCGATCTCAAAGTGCCCGCCGTTCGAGTTCGCCGGCGAATACTACAGCGCGGCGGCAGCCGGCTGCGTGCGGCGGAGCAGCTTCTTCGACGACAGGGCTGTTCTTGATCAGGGCGTTGGATACTCTGTTATTCTTGGCTTTGGTGCTTTTTTCGCCGTCTTCACTTCTTTTCTGGTCAGCTTCCTCTCTGAGTCTTCTCCTTTCAGCTCTGATCGGTCCTTGGAATTTTAGTATGCAATATAGTTTCCTAGTTTTAAATTTCCAACAATTTAATTCCTAAATTTTAGCATATAAAGATTAAGTCTTCGCTCTCAGCTAAATGGAGGAAGTATTGTGTCCTCTTAGTCACCTTGACCAATCACGTATTGATCCATCTAGTAATTGCTTTGATCCGTTACACATCAGCTGAAAAGTAGTCGTTTCCTTTGTGAGCTTCAGACAAGGGCCAGCAAGTCTTCTCCGAAAAAACTCTCGCAGTTCTACATCTTAATATAAATATACTATTTTTAAGATACAAACTTTGTAGTTAGATCCAATTCAAGACTAAAATAATATTTCATTATCCACGTGAGTATGAGTATAATTCAGCTGTAAATGACATCTACTCCTTTTTTTTTAATGGATGGTTAAACTTTAGGTATGGTTGGAGAATCAGTACATAGGGAGTCGACATACGTCTGAATGGTTCAACACAGCGGGCAGGAGCGTCAAGACAGGCCTCATTGCCAGTGTCATAGTCTCTCAGGTAAATATCCATTTCTTTTTTTCTCTGGATTTCATATTTGAATTTAGAGCCGTTGGGTTTTCTGAATTAATGGTTTTTTTTTTTTTTGTGTTTTTGTTGTGAAGTGGACATGGGCTGCAACCATCTTACAGAGTTCTAATGTTGCTTGGGAGTATGGAGTTAGTGGACCATTCTGGTATGCTAGTGGGGCTACCATTCAGGCAAGTTCGCCTCTCCCGAATGTCTATTTAATTTCTAAACTTTAACAAGATTTCAGTTACATCCTTGAACTTTATCACTATCGTTAATATCATGACTCAAATGTTATATAAGGGTTGTTGGCCAAAAAGTTCAATTTAAACTATTTTATCTAGATGGTTTACTCTCCATGTCACTTCGTACTGAACCATCATATGTTAATCTAATTGGTATGTCATTTCAACATTTAAGTCACAAAAATAACAATAAGGATGGTGTATGGAATCAAAATCAAAAGTTTGTGGACATAATTAAGACTTTCTAACGTTGGGAGGTTAGGTAGAAATATCTGTCAAGATTTAACCATTTTTACATTTTGGGTTTCACTTCCAAGAAAACAATTTCTAAGTTTCCATGATCAAAATTGTAAAAGGAAACACAATTACCAGTCAAGCTTCAAGTTTCAAAGGGACTAATGGTGCAATGTATAACAAAGGAAACGAGTACATTTACAGACTAAGAAGCATGAACACTCCAATTTGGGTAACATATTTGTGTCGAACTCCGACACTTATATTAGTTCAATAAATGTGTGTCACACACTGATTGTACAAAGTAAATATAGATCTGAAACTTGTGAGGCATTGAACCCTTGTAAACATAAGAAATATACATAAATAGTAATATAGGACAAAGTACAAATTTTGAAAGCAAAACACATCAAACTCATTTTTCTAAGCATGAAAATGCATAAATTTATTGACTTTCAATTTTATTCTGGTATAAAAACAATATATATTTAAAAAATTGTATATTTTAATAAACATGCATTTGTTATGTCCATGTCCTAAAATTGTAGAAAATTACGTGTGTGAATGTATCCATGTTGTGTCATATTCGTGACTCGTATCCGTATCCGTGCTTCTTACTTTACAAATGATTCAGGTTCTTCTATTTGGAATAATGGCTATAGAGATCAAAAGGAAGGCCCCAAATGCTCATACCGTTTGTGAAATTGTAAAGGCTCGGTAAGTTGTGAAACTCCATCTCCTTTCCATGTTATTTTACAAGTTTTTATTCAGCTTCTGCGGCCATGCCTCTTATTTTAGCTACAATTCTTTGTGGTATCTATCAGTTGGGGAACGGCTGCACATATTGTCTTCCTTGTTTTCTGCTTCCTCACCAACATCATAGTAACAGCCATGTTGCTCCTCGGCGGTTCGGCCGTGGTTAACGCGCTCACCGGAGTGAACATTTTTGCTGCAAGCTTTCTGATACCGCTCGGAGTCATCGTTTATACACTGGCTGGAGGATTAAAAGCCACTTTCTTGGCCAGCTATATACATTCTGTGATAGGTATTCTATGGACTTCTGAAAAACTTGAAAAGTGCTTCTAACCAGTTAAAAGCACTTTTCAAAGTTTTCGTGGTATTCGGTTCCATAGTAAAAAACGAGTTTAAAAACTCTAAAAACACTTTAAAAAAGCTATTAATCTCAGCCTGTTGTTTTGGCTGCAGTTCATGTGGTTTTGGTCATATTTGTGTTCCTAGTTTATACATCAAGCAGCCGGCTAGGGAGCCCCAGTGTTGTGTATAATCGTTTGATGGAGGTAGCAAGCAAATCCAGAATATGCCATGAACCAATTTCCCACATTGGCCAATCTTGTGGCCCTGTTGCTGGAAACTACAAAGGCTCTTATGCAACAATGTTGAGTTCCGGAGGGCTCGTTTTCGGGATTATTAATATCGTAGGCAACTTCGGTACTGTTTTCGTTGACAATGTGAGCAGCTTTAAGTTCGACAATGTTTAATTATCTCGAATGTATTCCGACTTAGGAAATGGCTCTCTGTTTGATCTAAGTTTCCCATATGATGCAAATTTCAGGGATATTGGATGAGTGCAATAGCTGCAAGACCCTCATCAACTCACAAGGGATATTTGGTGGGAGGCTTGGTCTGGTTTGCAGTTCCATTCTCTTTAGCAACTTCCCTCGGTCTTGGAGCGCTCGCCCTTGATTTACCGATTACTGCACAGGAGGCTAGTCGGGGACTCGTGCCTCCTGCCACGGCTATAGCGTTGATGGGGAAAGGAGGATCTATTCTTCTCCTTATCATGCTTTTTATGTAAGTTAGAAGAAGATATTCAATTAGAACTAACATTGATGGATATGATTCCTTTAATCTTAATGAACAGGGCTGTAACTTCTGCTGGTTCATCCGAGTTAATAAGCATATCATCCTTGTGCACGTACGATATCTACCGAACGTACGTAAACCCTGACGCGAGCGGAAAAAGCATCCTGAGAGTATCAAGAGCTGTTATTCTAGTGTTTGGATGTTTAATGGGACTGTTGGCCATACTTCTAAACAAGGCAGGAGTTTCATTAGGATGGATGTACCTTGCCATGGGAGTGCTGATTGGCTCAGCAGTTCTTCCTATTGCTTTCATGCTCCTGTGGAGTAAAGCGAATTCGATCGGAGCAATTCTAGGAACTACCATTGGATGTGTTCTAGGAATCGTTACGTGGGTCTCGGTCGCAAAGATCGAATATGGAAACGTTAACTTGGATACGACGGGTAGAAATGCACCTATGCTTGCTGGAAATCTTGTATCTATACTCTCAGGTGGCGCTATTCACGTCATATTTAGCCTGGTGTGGCCTCAAAACTATGACTGGGAGAGCACTAGGGAGATTTCAATGGTTGAAAAGGACAAATCTGAACTTCCATCCGAAGAATACAAAGAGGAAAAATTGATCAGGGCAAAAGTTTGGATAATGAAATGGGGTGTTGGATTTACCGTAGTAATTGTTATATTGTGGCCTCTTCTGTCTCTTCCAATAGGTAAATTTCTTTTACCTTGTCCATACTTTTGTTATACTTTTGGTGTTTGATCTTGTCTTCCTCCTCTTTGTGCAGGAGAGTTTAGTAAAGGATATTTCACACTTTGGGCAGTAATAGCAATAGCTTGGGGAACTATTGGTTCTGCTGTAATTATTTTCTTACCATTGATAGAAAGTTGGGAGACCATACAGAATGTGTTGCTTGGAATGTTTACAAACGATCGACTCGTCGAGAAGGTCGAAGAAATGAACCTGAAGTTGCATGCTTTAGTCTTGGCTCTACCTGAAGCAGAGAGAATTTACTTGCTCGAGAAGGAGAAGGCTAGGAAAAAGGATTTGTTAGATACAGATACACAGGCTCATCATGTTTAGGGACCTAAACAGCTCTAAATTTGTTCGAAAAGTTTGATATCTTTGGGCAGTCTCTGCATCCTTCCTTCCTTGTCACTAAACCGTCATTTAGGGACCTTAGATGATGATCCGAAAAAATCTCTCTTGTAATTTCAAAGGTTTTTCTGACTCATTTCAAGAAGGTTTAAATCACATTCGACCTAGATTCTTCTTAAGATTTTTTTTTTTTTTGTTGCCTGATTGATTTTGGTTCTTTTAACTTTTCAAAAAAGTAATCATACTCAGCCTCAAGTACATTATC

mRNA sequence

TGCCATTAAGATTGTGAAATCAAACTTGACTCGCTGACTCGGTAATAATTACCACGTATCTCAAACTAAACTAAGAGCCGGCTCTGAGTTGGATCATTTAAAAAGGGACTTCCGAATAAACGAAGAAAGATATTTCAAAATCTCAATAATACATTGTCCAAAGACTTTTCTCACTTCTTCTTCTTGTTCTTCAAAGTTGTGGTGTGTAGCAGCAGCAGAGATTGTGAAGCTTTTGCGGTGGTTTCATCAGCCATGGCTGCTCCGATCTCAAAGTGCCCGCCGTTCGAGTTCGCCGGCGAATACTACAGCGCGGCGGCAGCCGGCTGCGTGCGGCGGAGCAGCTTCTTCGACGACAGGGCTGTTCTTGATCAGGGCGTTGGATACTCTGTTATTCTTGGCTTTGGTGCTTTTTTCGCCGTCTTCACTTCTTTTCTGGTATGGTTGGAGAATCAGTACATAGGGAGTCGACATACGTCTGAATGGTTCAACACAGCGGGCAGGAGCGTCAAGACAGGCCTCATTGCCAGTGTCATAGTCTCTCAGTGGACATGGGCTGCAACCATCTTACAGAGTTCTAATGTTGCTTGGGAGTATGGAGTTAGTGGACCATTCTGGTATGCTAGTGGGGCTACCATTCAGGCAAGTCTTCTATTTGGAATAATGGCTATAGAGATCAAAAGGAAGGCCCCAAATGCTCATACCGTTTGTGAAATTGTAAAGGCTCGTTGGGGAACGGCTGCACATATTGTCTTCCTTGTTTTCTGCTTCCTCACCAACATCATAGTAACAGCCATGTTGCTCCTCGGCGGTTCGGCCGTGGTTAACGCGCTCACCGGAGTGAACATTTTTGCTGCAAGCTTTCTGATACCGCTCGGAGTCATCGTTTATACACTGGCTGGAGGATTAAAAGCCACTTTCTTGGCCAGCTATATACATTCTGTGATAGTTCATGTGGTTTTGGTCATATTTGTGTTCCTAGTTTATACATCAAGCAGCCGGCTAGGGAGCCCCAGTGTTGTGTATAATCGTTTGATGGAGGTAGCAAGCAAATCCAGAATATGCCATGAACCAATTTCCCACATTGGCCAATCTTGTGGCCCTGTTGCTGGAAACTACAAAGGCTCTTATGCAACAATGTTGAGTTCCGGAGGGCTCGTTTTCGGGATTATTAATATCGTAGGCAACTTCGGTACTGTTTTCGTTGACAATGGATATTGGATGAGTGCAATAGCTGCAAGACCCTCATCAACTCACAAGGGATATTTGGTGGGAGGCTTGGTCTGGTTTGCAGTTCCATTCTCTTTAGCAACTTCCCTCGGTCTTGGAGCGCTCGCCCTTGATTTACCGATTACTGCACAGGAGGCTAGTCGGGGACTCGTGCCTCCTGCCACGGCTATAGCGTTGATGGGGAAAGGAGGATCTATTCTTCTCCTTATCATGCTTTTTATGGCTGTAACTTCTGCTGGTTCATCCGAGTTAATAAGCATATCATCCTTGTGCACGTACGATATCTACCGAACGTACGTAAACCCTGACGCGAGCGGAAAAAGCATCCTGAGAGTATCAAGAGCTGTTATTCTAGTGTTTGGATGTTTAATGGGACTGTTGGCCATACTTCTAAACAAGGCAGGAGTTTCATTAGGATGGATGTACCTTGCCATGGGAGTGCTGATTGGCTCAGCAGTTCTTCCTATTGCTTTCATGCTCCTGTGGAGTAAAGCGAATTCGATCGGAGCAATTCTAGGAACTACCATTGGATGTGTTCTAGGAATCGTTACGTGGGTCTCGGTCGCAAAGATCGAATATGGAAACGTTAACTTGGATACGACGGGTAGAAATGCACCTATGCTTGCTGGAAATCTTGTATCTATACTCTCAGGTGGCGCTATTCACGTCATATTTAGCCTGGTGTGGCCTCAAAACTATGACTGGGAGAGCACTAGGGAGATTTCAATGGTTGAAAAGGACAAATCTGAACTTCCATCCGAAGAATACAAAGAGGAAAAATTGATCAGGGCAAAAGTTTGGATAATGAAATGGGGTGTTGGATTTACCGTAGTAATTGTTATATTGTGGCCTCTTCTGTCTCTTCCAATAGGAGAGTTTAGTAAAGGATATTTCACACTTTGGGCAGTAATAGCAATAGCTTGGGGAACTATTGGTTCTGCTGTAATTATTTTCTTACCATTGATAGAAAGTTGGGAGACCATACAGAATGTGTTGCTTGGAATGTTTACAAACGATCGACTCGTCGAGAAGGTCGAAGAAATGAACCTGAAGTTGCATGCTTTAGTCTTGGCTCTACCTGAAGCAGAGAGAATTTACTTGCTCGAGAAGGAGAAGGCTAGGAAAAAGGATTTGTTAGATACAGATACACAGGCTCATCATGTTTAGGGACCTAAACAGCTCTAAATTTGTTCGAAAAGTTTGATATCTTTGGGCAGTCTCTGCATCCTTCCTTCCTTGTCACTAAACCGTCATTTAGGGACCTTAGATGATGATCCGAAAAAATCTCTCTTGTAATTTCAAAGGTTTTTCTGACTCATTTCAAGAAGGTTTAAATCACATTCGACCTAGATTCTTCTTAAGATTTTTTTTTTTTTTGTTGCCTGATTGATTTTGGTTCTTTTAACTTTTCAAAAAAGTAATCATACTCAGCCTCAAGTACATTATC

Coding sequence (CDS)

ATGGCTGCTCCGATCTCAAAGTGCCCGCCGTTCGAGTTCGCCGGCGAATACTACAGCGCGGCGGCAGCCGGCTGCGTGCGGCGGAGCAGCTTCTTCGACGACAGGGCTGTTCTTGATCAGGGCGTTGGATACTCTGTTATTCTTGGCTTTGGTGCTTTTTTCGCCGTCTTCACTTCTTTTCTGGTATGGTTGGAGAATCAGTACATAGGGAGTCGACATACGTCTGAATGGTTCAACACAGCGGGCAGGAGCGTCAAGACAGGCCTCATTGCCAGTGTCATAGTCTCTCAGTGGACATGGGCTGCAACCATCTTACAGAGTTCTAATGTTGCTTGGGAGTATGGAGTTAGTGGACCATTCTGGTATGCTAGTGGGGCTACCATTCAGGCAAGTCTTCTATTTGGAATAATGGCTATAGAGATCAAAAGGAAGGCCCCAAATGCTCATACCGTTTGTGAAATTGTAAAGGCTCGTTGGGGAACGGCTGCACATATTGTCTTCCTTGTTTTCTGCTTCCTCACCAACATCATAGTAACAGCCATGTTGCTCCTCGGCGGTTCGGCCGTGGTTAACGCGCTCACCGGAGTGAACATTTTTGCTGCAAGCTTTCTGATACCGCTCGGAGTCATCGTTTATACACTGGCTGGAGGATTAAAAGCCACTTTCTTGGCCAGCTATATACATTCTGTGATAGTTCATGTGGTTTTGGTCATATTTGTGTTCCTAGTTTATACATCAAGCAGCCGGCTAGGGAGCCCCAGTGTTGTGTATAATCGTTTGATGGAGGTAGCAAGCAAATCCAGAATATGCCATGAACCAATTTCCCACATTGGCCAATCTTGTGGCCCTGTTGCTGGAAACTACAAAGGCTCTTATGCAACAATGTTGAGTTCCGGAGGGCTCGTTTTCGGGATTATTAATATCGTAGGCAACTTCGGTACTGTTTTCGTTGACAATGGATATTGGATGAGTGCAATAGCTGCAAGACCCTCATCAACTCACAAGGGATATTTGGTGGGAGGCTTGGTCTGGTTTGCAGTTCCATTCTCTTTAGCAACTTCCCTCGGTCTTGGAGCGCTCGCCCTTGATTTACCGATTACTGCACAGGAGGCTAGTCGGGGACTCGTGCCTCCTGCCACGGCTATAGCGTTGATGGGGAAAGGAGGATCTATTCTTCTCCTTATCATGCTTTTTATGGCTGTAACTTCTGCTGGTTCATCCGAGTTAATAAGCATATCATCCTTGTGCACGTACGATATCTACCGAACGTACGTAAACCCTGACGCGAGCGGAAAAAGCATCCTGAGAGTATCAAGAGCTGTTATTCTAGTGTTTGGATGTTTAATGGGACTGTTGGCCATACTTCTAAACAAGGCAGGAGTTTCATTAGGATGGATGTACCTTGCCATGGGAGTGCTGATTGGCTCAGCAGTTCTTCCTATTGCTTTCATGCTCCTGTGGAGTAAAGCGAATTCGATCGGAGCAATTCTAGGAACTACCATTGGATGTGTTCTAGGAATCGTTACGTGGGTCTCGGTCGCAAAGATCGAATATGGAAACGTTAACTTGGATACGACGGGTAGAAATGCACCTATGCTTGCTGGAAATCTTGTATCTATACTCTCAGGTGGCGCTATTCACGTCATATTTAGCCTGGTGTGGCCTCAAAACTATGACTGGGAGAGCACTAGGGAGATTTCAATGGTTGAAAAGGACAAATCTGAACTTCCATCCGAAGAATACAAAGAGGAAAAATTGATCAGGGCAAAAGTTTGGATAATGAAATGGGGTGTTGGATTTACCGTAGTAATTGTTATATTGTGGCCTCTTCTGTCTCTTCCAATAGGAGAGTTTAGTAAAGGATATTTCACACTTTGGGCAGTAATAGCAATAGCTTGGGGAACTATTGGTTCTGCTGTAATTATTTTCTTACCATTGATAGAAAGTTGGGAGACCATACAGAATGTGTTGCTTGGAATGTTTACAAACGATCGACTCGTCGAGAAGGTCGAAGAAATGAACCTGAAGTTGCATGCTTTAGTCTTGGCTCTACCTGAAGCAGAGAGAATTTACTTGCTCGAGAAGGAGAAGGCTAGGAAAAAGGATTTGTTAGATACAGATACACAGGCTCATCATGTTTAG

Protein sequence

MAAPISKCPPFEFAGEYYSAAAAGCVRRSSFFDDRAVLDQGVGYSVILGFGAFFAVFTSFLVWLENQYIGSRHTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGPFWYASGATIQASLLFGIMAIEIKRKAPNAHTVCEIVKARWGTAAHIVFLVFCFLTNIIVTAMLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHVVLVIFVFLVYTSSSRLGSPSVVYNRLMEVASKSRICHEPISHIGQSCGPVAGNYKGSYATMLSSGGLVFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGALALDLPITAQEASRGLVPPATAIALMGKGGSILLLIMLFMAVTSAGSSELISISSLCTYDIYRTYVNPDASGKSILRVSRAVILVFGCLMGLLAILLNKAGVSLGWMYLAMGVLIGSAVLPIAFMLLWSKANSIGAILGTTIGCVLGIVTWVSVAKIEYGNVNLDTTGRNAPMLAGNLVSILSGGAIHVIFSLVWPQNYDWESTREISMVEKDKSELPSEEYKEEKLIRAKVWIMKWGVGFTVVIVILWPLLSLPIGEFSKGYFTLWAVIAIAWGTIGSAVIIFLPLIESWETIQNVLLGMFTNDRLVEKVEEMNLKLHALVLALPEAERIYLLEKEKARKKDLLDTDTQAHHV
Homology
BLAST of MC10g1384 vs. ExPASy Swiss-Prot
Match: F4KD71 (Urea-proton symporter DUR3 OS=Arabidopsis thaliana OX=3702 GN=DUR3 PE=1 SV=1)

HSP 1 Score: 1060.4 bits (2741), Expect = 8.7e-309
Identity = 533/703 (75.82%), Postives = 622/703 (88.48%), Query Frame = 0

Query: 5   ISKCPPFEFAGEYYSAAAAGCVRRSSFFDDRAVLDQGVGYSVILGFGAFFAVFTSFLVWL 64
           ++ CPPF+F+ +YY     GC R+SSFF    VLDQGVGY+VILGFGAFFAVFTSFLVWL
Sbjct: 1   MATCPPFDFSTKYYD-GDGGCQRQSSFFGGTTVLDQGVGYAVILGFGAFFAVFTSFLVWL 60

Query: 65  ENQYIGSRHTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGPFWYAS 124
           E +Y+G+RHTSEWFNTAGR+VKTGLIASVIVSQWTWAATILQSSNVAW+YGVSGPFWYAS
Sbjct: 61  EKRYVGARHTSEWFNTAGRNVKTGLIASVIVSQWTWAATILQSSNVAWQYGVSGPFWYAS 120

Query: 125 GATIQASLLFGIMAIEIKRKAPNAHTVCEIVKARWGTAAHIVFLVFCFLTNIIVTAMLLL 184
           GATIQ  LLFG+MAIEIKRKAPNAHTVCEIVKARWGTA HIVFLVFC  TN++VTAMLLL
Sbjct: 121 GATIQV-LLFGVMAIEIKRKAPNAHTVCEIVKARWGTATHIVFLVFCLATNVVVTAMLLL 180

Query: 185 GGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHVVLVIFVFLVY 244
           GGSAVVNALTGVN++AASFLIPLGV+VYTLAGGLKATFLASY+HSVIVHV LV+FVFLVY
Sbjct: 181 GGSAVVNALTGVNLYAASFLIPLGVVVYTLAGGLKATFLASYVHSVIVHVALVVFVFLVY 240

Query: 245 TSSSRLGSPSVVYNRLMEVASKSRICHEPISHIGQSCGPVAGNYKGSYATMLSSGGLVFG 304
           TSS  LGSPSVVY+RL ++ +KSR C EP+SH GQ+CGPV GN++GSY TMLSSGG VFG
Sbjct: 241 TSSKELGSPSVVYDRLKDMVAKSRSCTEPLSHHGQACGPVDGNFRGSYLTMLSSGGAVFG 300

Query: 305 IINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGALALDL 364
           +INIVGNFGTVFVDNGYW+SAIAARPSSTHKGYL+GGLVWFAVPFSLATSLGLGALALDL
Sbjct: 301 LINIVGNFGTVFVDNGYWVSAIAARPSSTHKGYLLGGLVWFAVPFSLATSLGLGALALDL 360

Query: 365 PITAQEASRGLVPPATAIALMGKGGSILLLIMLFMAVTSAGSSELISISSLCTYDIYRTY 424
           PI+  EA RGLVPPATAIALMGK GS+LLL MLFMAVTSAGSSELI++SSL TYDIYRTY
Sbjct: 361 PISKDEADRGLVPPATAIALMGKSGSLLLLTMLFMAVTSAGSSELIAVSSLFTYDIYRTY 420

Query: 425 VNPDASGKSILRVSRAVILVFGCLMGLLAILLNKAGVSLGWMYLAMGVLIGSAVLPIAFM 484
           +NP A+G+ IL++SR  +L FGC MG+LA++LNKAGVSLGWMYLAMGVLIGSAV+PIAFM
Sbjct: 421 INPRATGRQILKISRCAVLGFGCFMGILAVVLNKAGVSLGWMYLAMGVLIGSAVIPIAFM 480

Query: 485 LLWSKANSIGAILGTTIGCVLGIVTWVSVAKIEYGNVNLDTTGRNAPMLAGNLVSILSGG 544
           LLWSKAN+ GAILG T GCV GI+TW++ AK +YG V+LD+TG+N PMLAGNLV+IL+GG
Sbjct: 481 LLWSKANAFGAILGATSGCVFGIITWLTTAKTQYGRVDLDSTGKNGPMLAGNLVAILTGG 540

Query: 545 AIHVIFSLVWPQNYDWESTREISMVEKDKS-----ELPSEEYKEEKLIRAKVWIMKWGVG 604
            IH + SLV PQNYDW +TREI +VE   S     ++P+EE +EEKL RAK WI+KWG+ 
Sbjct: 541 LIHAVCSLVRPQNYDWSTTREIKVVEAYASGDEDVDVPAEELREEKLRRAKAWIVKWGLV 600

Query: 605 FTVVIVILWPLLSLPIGEFSKGYFTLWAVIAIAWGTIGSAVIIFLPLIESWETIQNVLLG 664
           FT++IV++WP+LSLP   FS+GYF  WA++AIAWGTIGS VII LPL+ESW+TI++V +G
Sbjct: 601 FTILIVVIWPVLSLPARVFSRGYFWFWAIVAIAWGTIGSIVIIGLPLVESWDTIKSVCMG 660

Query: 665 MFTNDRLVEKVEEMNLKLHALVLALPEAERIYLLEKEKARKKD 703
           MFTNDR+++K++++N +L AL +A+PEAE+IYLLE EK +K D
Sbjct: 661 MFTNDRVMKKLDDLNHRLRALTMAVPEAEKIYLLELEKTKKND 701

BLAST of MC10g1384 vs. ExPASy Swiss-Prot
Match: Q7XBS0 (Urea-proton symporter DUR3 OS=Oryza sativa subsp. japonica OX=39947 GN=DUR3 PE=1 SV=1)

HSP 1 Score: 1012.3 bits (2616), Expect = 2.7e-294
Identity = 512/686 (74.64%), Postives = 591/686 (86.15%), Query Frame = 0

Query: 8   CPPFE--FAGEYYSAAAAGCVRRSSFFDDRAVLDQGVGYSVILGFGAFFAVFTSFLVWLE 67
           CPP E  F  EYYS     C R  S+F  R VL Q VGY+V+LGFGAFFA+FTSFLVWLE
Sbjct: 6   CPPAELGFGAEYYSVVNGVCSRAGSYFGGRPVLTQAVGYAVVLGFGAFFALFTSFLVWLE 65

Query: 68  NQYIGSRHTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGPFWYASG 127
            +Y+GS+HTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAW+YGVSGPFWYASG
Sbjct: 66  KRYVGSQHTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWQYGVSGPFWYASG 125

Query: 128 ATIQASLLFGIMAIEIKRKAPNAHTVCEIVKARWGTAAHIVFLVFCFLTNIIVTAMLLLG 187
           ATIQ  LLFG+MAIEIKRKAPNAHTVCEIV+ARWGT AH+VFL FC LTN+IVTAMLLLG
Sbjct: 126 ATIQV-LLFGVMAIEIKRKAPNAHTVCEIVRARWGTPAHLVFLTFCLLTNVIVTAMLLLG 185

Query: 188 GSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHVVLVIFVFLVYT 247
           GSAVVNALTGVN++AASFLIPLGV+VYTLAGGLKATFLASYIHSV+VH VLV+FVFLVYT
Sbjct: 186 GSAVVNALTGVNVYAASFLIPLGVVVYTLAGGLKATFLASYIHSVVVHAVLVVFVFLVYT 245

Query: 248 SSSRLGSPSVVYNRLMEVASKSRICHEPISHIGQSCGPVAGNYKGSYATMLSSGGLVFGI 307
           SSS+LGSP VVY+RLM VAS +R C   +S  GQ+CGPVAGN+KGSY TMLSSGGLVFGI
Sbjct: 246 SSSKLGSPRVVYDRLMAVASAARDCSADLSRNGQACGPVAGNFKGSYLTMLSSGGLVFGI 305

Query: 308 INIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGALALDLP 367
           INIVGNFGTVFVDNGYWMSAIAARPSSTHKGYL+GGLVWFAVPFSLATSLGLGALALDLP
Sbjct: 306 INIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLLGGLVWFAVPFSLATSLGLGALALDLP 365

Query: 368 ITAQEASRGLVPPATAIALMGKGGSILLLIMLFMAVTSAGSSELISISSLCTYDIYRTYV 427
           +TA EA++GLVPPATA ALMGK GS+LLL MLFMAVTSAGS+EL+++SSLCTYDIYRTY+
Sbjct: 366 LTAAEAAKGLVPPATATALMGKSGSVLLLTMLFMAVTSAGSAELVAVSSLCTYDIYRTYL 425

Query: 428 NPDASGKSILRVSRAVILVFGCLMGLLAILLNKAGVSLGWMYLAMGVLIGSAVLPIAFML 487
           NP ASGK ILRVSRAV+L FGC MG+LA++LN AGVSLGWMYLAMGV++GSAV+PIA +L
Sbjct: 426 NPGASGKQILRVSRAVVLGFGCFMGVLAVVLNVAGVSLGWMYLAMGVIVGSAVIPIALLL 485

Query: 488 LWSKANSIGAILGTTIGCVLGIVTWVSVAKIEYGNVNLDTTGRNAPMLAGNLVSILSGGA 547
           LWSKAN++GA+ G   GC LG+  W++VAK++YG VNLDTTGRNAPMLAGNLVSIL GGA
Sbjct: 486 LWSKANAVGAMGGAVSGCALGVAVWLTVAKVQYGRVNLDTTGRNAPMLAGNLVSILVGGA 545

Query: 548 IHVIFSLVWPQNYDWESTREISMVEKDKSELPSEEYKEEKLIRAKVWIMKWGVGFTVVIV 607
           +H   SL+ PQ+YDW ++RE+    +       +E KEE+L+ AK WI++WG+ FT VIV
Sbjct: 546 VHAACSLLRPQHYDWGTSREMITTVESVHAALDDELKEERLVHAKRWIVRWGLVFTAVIV 605

Query: 608 ILWPLLSLPIGEFSKGYFTLWAVIAIAWGTIGSAVIIFLPLIESWETIQNVLLGMFTNDR 667
           + WP LSLP   +S GYFTLWA +AIAWGT+GS VII LP+ ESW TI  V  GMFTND 
Sbjct: 606 VAWPALSLPARRYSLGYFTLWAAVAIAWGTVGSVVIILLPVAESWTTITKVCAGMFTNDA 665

Query: 668 LVEKVEEMNLKLHALVLALPEAERIY 692
           + ++++++NL+L A++ A+PEAE+ Y
Sbjct: 666 VYDRLDDVNLRLRAIMGAMPEAEKRY 690

BLAST of MC10g1384 vs. ExPASy Swiss-Prot
Match: O94469 (Probable urea active transporter 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dur3-1 PE=3 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 1.6e-137
Identity = 290/674 (43.03%), Postives = 416/674 (61.72%), Query Frame = 0

Query: 38  LDQGVGYSVILGFGAFFAVFTSFLVWLENQYIGSRHTSEWFNTAGRSVKTGLIASVIVSQ 97
           L Q VGY +++G G  FA    F+ W   ++     TSE FNTA  SV+TGL+AS +VS 
Sbjct: 6   LTQSVGYGIVVGLGLGFAALMIFVSWSLKKFNNENQTSEHFNTASHSVRTGLVASAVVSS 65

Query: 98  WTWAATILQSSNVAWEYGVSGPFWYASGATIQASLLFGIMAIEIKRKAPNAHTVCEIVKA 157
           WTWA+T+L S+   ++YGVSG FWYASGA +Q  LLF ++AIE+KRKAPNAHT  E+V+A
Sbjct: 66  WTWASTLLTSAQKTYQYGVSGAFWYASGACVQI-LLFTVLAIELKRKAPNAHTFLEVVRA 125

Query: 158 RWGTAAHIVFLVFCFLTNIIVTAMLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGG 217
           R G  AH VFLVF ++TNI+V AMLL GGSA ++++TG+N  A  FL+P+GVI+YT+ GG
Sbjct: 126 RCGPIAHGVFLVFAYITNILVMAMLLCGGSATISSVTGMNTVAVCFLLPVGVIIYTMFGG 185

Query: 218 LKATFLASYIHSVIVHVVLVIFVFLVYTSSSRLGSPSVVYNRLMEVASKSRICHEPISHI 277
           +KATFL  YIH+VI+ V+L++F    Y++  ++GSP  +Y+ L E               
Sbjct: 186 IKATFLTDYIHTVIILVILIMFSLATYSADKKIGSPGKLYDMLKEAG------------- 245

Query: 278 GQSCGPVAGNYKGSYATMLSSGGLVFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGY 337
                PVAGN +GSY TM S  G +F IIN+ GNFGTVFVDNGYW  AIAA P+S   GY
Sbjct: 246 --DAHPVAGNAQGSYLTMRSQEGAIFFIINLAGNFGTVFVDNGYWQKAIAANPASALPGY 305

Query: 338 LVGGLVWFAVPFSLATSLGLGALALD----LP-----ITAQEASRGLVPPATAIALMGKG 397
           ++GGL WFA+P+  AT++GL AL L+     P     ++  E S GLV P  AIALMG+ 
Sbjct: 306 ILGGLAWFAIPWLAATTMGLVALGLENKPYFPTYPNRMSDLEVSEGLVLPYAAIALMGRA 365

Query: 398 GSILLLIMLFMAVTSAGSSELISISSLCTYDIYRTYVNPDASGKSILRVSRAVILVFGCL 457
           G+   L+++FMAVTSA S+ELI++SS+ TYDIY+ YV P A+GK +L    A ++VFG  
Sbjct: 366 GANATLLLVFMAVTSAASAELIAVSSIFTYDIYKQYVRPRATGKELLYTGHASLIVFGFA 425

Query: 458 MGLLAILLNKAGVSLGWMYLAMGVLIGSAVLPIAFMLLWSKANSIGAILGTTIGCVLGIV 517
           M   A  L    VS+G++YL MGVL+  AV+P   ++L+S+ ++I   +   +G +  I+
Sbjct: 426 MSGFATGLYYGQVSMGYLYLLMGVLVCPAVVPATCVMLFSRVSTIAVTVSPVLGIISSII 485

Query: 518 TWVSVAKIEYG-NVNLDTTGRNAPMLAGNLVSILSGGAIHVIFSLVWPQNYDWESTREIS 577
           TW+ VA+ E G  + ++TTG N PMLAGN+V +LS     +I S+++P+ YD+   R ++
Sbjct: 486 TWLVVARAEGGKTLTIETTGANNPMLAGNVVGLLSPALYILILSIIFPEKYDF--NRLLA 545

Query: 578 MVEKDKSELPSEEYKEEKLIRAKVWIMKWGVGFTVVIVILWPLLSLPIGE-FSKGYFTLW 637
                 S    E  + +KL RA V      +  T   +ILWP      G  FSK +FT W
Sbjct: 546 TFAMHFSSEEDEIQQTKKLNRASVISKVAALIITAAFIILWPWPMYGTGYIFSKRFFTGW 605

Query: 638 AVIAIAWGTIGSAVIIFLPLIES----WETIQNVLLGMFTNDRLVEKVEEMNLKLHALVL 697
            V+ + W       +   PL E     ++ + N+   +F   ++ + VE+  + +  + +
Sbjct: 606 VVVGLIWIFFTVFAVGIFPLWEGRNDIYQVVSNMAASIF-GRKVNDIVEDEGVVVETISI 660

BLAST of MC10g1384 vs. ExPASy Swiss-Prot
Match: P33413 (Urea active transporter OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DUR3 PE=1 SV=2)

HSP 1 Score: 436.8 bits (1122), Expect = 4.7e-121
Identity = 268/698 (38.40%), Postives = 393/698 (56.30%), Query Frame = 0

Query: 38  LDQGVGYSVILGFGAFFAVFTSFLVWLENQYIGSRHTSEWFNTAGRSVKTGLIASVIVSQ 97
           L QG GY+++LG GA FA       +L  +Y     T+E F TAGRSVKTGL+A+ +VS 
Sbjct: 8   LPQGAGYAIVLGLGAVFAGMMVLTTYLLKRYQKEIITAEEFTTAGRSVKTGLVAAAVVSS 67

Query: 98  WTWAATILQSSNVAWEYGVSGPFWYASGATIQASLLFGIMAIEIKRKAPNAHTVCEIVKA 157
           W W +T+L SS   +  G+ G + YA+GA  Q  + F I+AI+ K+ APNAHT  E+V+ 
Sbjct: 68  WIWCSTLLTSSTKEYADGIFGGYAYAAGACFQI-IAFAILAIKTKQMAPNAHTYLELVRT 127

Query: 158 RWGTAAHIVFLVFCFLTNIIVTAMLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGG 217
           R+G   H  +L +   TNI+VT+MLL  GSAV + LTG+N  A+ FL+P+GV+VYTL GG
Sbjct: 128 RYGKIGHGCYLFYAIATNILVTSMLLTSGSAVFSDLTGMNTIASCFLLPVGVVVYTLFGG 187

Query: 218 LKATFLASYIHSVIVHVVLVIFVFLVYTSSSRLGSPSVVYNRLMEVASKSRICHEPISHI 277
           +KATFL  Y+H+ ++ +++++F F VY +S  LGSP  VY+ + E A +           
Sbjct: 188 IKATFLTDYMHTCVIIIIVLVFAFKVYATSDVLGSPGKVYDLVREAAKRH---------- 247

Query: 278 GQSCGPVAGNYKGSYATMLSSGGLVFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGY 337
                PV GNY+G Y TM S    +  IIN++GNFGTVF+DNGYW  AI+A P+++ K Y
Sbjct: 248 -----PVDGNYQGEYMTMTSKSAGILLIINLIGNFGTVFLDNGYWNKAISASPAASLKAY 307

Query: 338 LVGGLVWFAVPFSLATSLGLGALALDL---------PITAQEASRGLVPPATAIALMGKG 397
            +GGL WFAVP  ++ ++GL  LA++          P+T+ +A+ GLV PA AIA+MGKG
Sbjct: 308 AIGGLAWFAVPSLISLTMGLACLAVETSPNFPTYPDPLTSFQANSGLVLPAAAIAIMGKG 367

Query: 398 GSILLLIMLFMAVTSAGSSELISISSLCTYDIYRTYVNPDASGKSILRVSRAVILVFGCL 457
           G++  L+M+FMAVTSA S+ELI++SS+ TYDIYR Y++P ASGK ++  S    + FG  
Sbjct: 368 GAVASLLMIFMAVTSAMSAELIAVSSVFTYDIYREYIDPRASGKKLIYTSHVACIFFGLA 427

Query: 458 MGLLAILLNKAGVSLGWMYLAMGVLIGSAVLPIAFMLLWSKANSIGAILGTTIGCVLGIV 517
           M   ++ L   G+S+G++Y  MG++I SAVLP+   L     N + A++   +G  L I+
Sbjct: 428 MSGFSVGLYYGGISMGYIYEMMGIIISSAVLPVVLTLCSKDMNLVAAVVSPILGTGLAIM 487

Query: 518 TWVSVAKIEYGNVNLDTTGRNAPMLAGNLVSILSGGAIHVIFSLVW-PQNYDWESTREIS 577
           +W+   K  Y  + +DTT  + PML GNLV++LS      I + V+ PQN+DWE  ++I+
Sbjct: 488 SWLVCTKSLYKELTVDTTFMDYPMLTGNLVALLSPAIFIPILTYVFKPQNFDWEKMKDIT 547

Query: 578 MV-------------------------EKDKSELPSEEYK------EEKLIRAKVWIMKW 637
            V                         E++ + L S+  K       EKLI   + ++  
Sbjct: 548 RVDETAELVQADPDIQLYDAEANDKEQEEETNSLVSDSEKNDVRVNNEKLIEPNLGVVIS 607

Query: 638 G--------------------------------VGFTVVIVILWPLLSLPIGE-FSKGYF 662
                                            V F +  +++WP+        FSK +F
Sbjct: 608 NAIFQEDDTQLQNELDEEQRELARGLKIAYFLCVFFALAFLVVWPMPMYGSKYIFSKKFF 667

BLAST of MC10g1384 vs. ExPASy Swiss-Prot
Match: Q9URY6 (Probable urea active transporter 3 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dur3-3 PE=3 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 2.9e-115
Identity = 252/632 (39.87%), Postives = 374/632 (59.18%), Query Frame = 0

Query: 37  VLDQGVGYSVILGFGAFFAVFTSFLVWLENQYIGSRHTSEWFNTAGRSVKTGLIASVIVS 96
           VL+QG GY +++G G  FA+    + ++  +Y+G    SE F TA RSVKTGLI+S +VS
Sbjct: 5   VLNQGYGYGIVIGLGFAFAIVMILVTYVLKRYVGEVQDSEHFTTASRSVKTGLISSAVVS 64

Query: 97  QWTWAATILQSSNVAWEYGVSGPFWYASGATIQASLLFGIMAIEIKRKAPNAHTVCEIVK 156
            WTW  T+L S+ +A+EYGV G  WY+   T+Q +  F ++A+++KR AP AHT+ EIVK
Sbjct: 65  SWTWPGTLLTSAGMAYEYGVCGSMWYSFAFTVQIT-FFTVIALQVKRVAPGAHTIVEIVK 124

Query: 157 ARWGTAAHIVFLVFCFLTNIIVTAMLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAG 216
           AR+G A+H VFL +   TNIIV+AMLLLGGS  ++A+TG+++ AA FL+PLGV +YT++G
Sbjct: 125 ARFGQASHAVFLFYALGTNIIVSAMLLLGGSQAISAITGMHVVAAGFLLPLGVWLYTVSG 184

Query: 217 GLKATFLASYIHSVIVHVVLVIFVFLVYTSSSRLGSPSVVYNRLMEVASKSRICHEPISH 276
           GLK+TFL+ + H+VIV++V++I +F+ YTSS  +GS   +Y+ L EV+  +         
Sbjct: 185 GLKSTFLSDWTHTVIVYIVILITLFVAYTSSVHIGSIDKMYDLLTEVSKTN--------- 244

Query: 277 IGQSCGPVAGNYKGSYATMLSSGGLVFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKG 336
                 P  G YKGSY T+ +   +  G   ++G F TVF D  Y   AIAA+P S  KG
Sbjct: 245 ------PSTG-YKGSYLTVTNRDAVFVGWNIVIGGFATVFCDPSYGQKAIAAKPISAMKG 304

Query: 337 YLVGGLVWFAVPFSLATSLGLGALALDL---------PITAQEASRGLVPPATAIALMGK 396
           Y  GGL W  VP+++ ++  L  LAL           P+++++ S G+       ALMGK
Sbjct: 305 YFAGGLAWLIVPWAMGSAAALSCLALTNNPVSVTYPDPVSSKQVSEGMPLLYGMTALMGK 364

Query: 397 GGSILLLIMLFMAVTSAGSSELISISSLCTYDIYRTYVNPDASGKSILRVSRAVILVFGC 456
            G+   +++LFMA TSA S+EL++ SS+ TYD+YR Y  P+ASGK ++RV+   + +F  
Sbjct: 365 NGAAAGVLILFMASTSATSAELVAFSSVMTYDVYRNYFRPNASGKELVRVTHVFVTIFAV 424

Query: 457 LMGLLAILLNKAGVSLGWMYLAMGVLIGSAVLPIAFMLLWSKANSIGAILGTTIGCVLGI 516
            MG LA+L N  G+++ W+   +G+ +G AV  I   L W K N  G I+G  +G + G+
Sbjct: 425 CMGALAVLFNYIGITISWIITFIGIALGPAVFGITLTLFWKKMNKYGMIIGCPMGSITGV 484

Query: 517 VTWV-SVAKIEYGNVNLDTTGRNAPMLAGNLVSILSGGAIHVIFSLVWPQNYDWESTREI 576
           V WV S  K   G VN  T         GN   + SG    V+ S  +P   D  +    
Sbjct: 485 VCWVGSCYKFSNGVVNKTTLNTPYANAVGNFTGLFSGLIYIVLISYFFPNKSDDLNNLNE 544

Query: 577 SMVEKDKS---ELPSEEYKEEKLIRA-KVWI-MKWGVGFTVVIVILWPLLSLPIGEFSKG 636
             V  D +   E+   E ++++L R+ ++ I + W + F +VI++  P+       FSK 
Sbjct: 545 KFVLGDDATAEEIVDAETEKKQLDRSLRIGIFVSWIIFFILVIIVPLPMYGSKY-IFSKL 604

Query: 637 YFTLWAVIAIAWGTIGSAVIIFLPLIESWETI 654
           +F  W ++ I W  I +  I F PL ES +TI
Sbjct: 605 FFRGWIIVIIIWTLIAALYITFYPLYESRDTI 618

BLAST of MC10g1384 vs. NCBI nr
Match: XP_022145424.1 (urea-proton symporter DUR3 [Momordica charantia])

HSP 1 Score: 1357 bits (3513), Expect = 0.0
Identity = 711/713 (99.72%), Postives = 711/713 (99.72%), Query Frame = 0

Query: 1   MAAPISKCPPFEFAGEYYSAAAAGCVRRSSFFDDRAVLDQGVGYSVILGFGAFFAVFTSF 60
           MAAPISKCPPFEFAGEYYSAAAAGCVRRSSFFDDRAVLDQGVGYSVILGFGAFFAVFTSF
Sbjct: 1   MAAPISKCPPFEFAGEYYSAAAAGCVRRSSFFDDRAVLDQGVGYSVILGFGAFFAVFTSF 60

Query: 61  LVWLENQYIGSRHTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGPF 120
           LVWLENQYIGSRHTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGPF
Sbjct: 61  LVWLENQYIGSRHTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGPF 120

Query: 121 WYASGATIQASLLFGIMAIEIKRKAPNAHTVCEIVKARWGTAAHIVFLVFCFLTNIIVTA 180
           WYASGATIQ  LLFGIMAIEIKRKAPNAHTVCEIVKARWGTAAHIVFLVFCFLTNIIVTA
Sbjct: 121 WYASGATIQV-LLFGIMAIEIKRKAPNAHTVCEIVKARWGTAAHIVFLVFCFLTNIIVTA 180

Query: 181 MLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHVVLVIFV 240
           MLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHVVLVIFV
Sbjct: 181 MLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHVVLVIFV 240

Query: 241 FLVYTSSSRLGSPSVVYNRLMEVASKSRICHEPISHIGQSCGPVAGNYKGSYATMLSSGG 300
           FLVYTSSSRLGSPSVVYNRLMEVASKSRICHEPISHIGQSCGPVAGNYKGSYATMLSSGG
Sbjct: 241 FLVYTSSSRLGSPSVVYNRLMEVASKSRICHEPISHIGQSCGPVAGNYKGSYATMLSSGG 300

Query: 301 LVFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGAL 360
           LVFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGAL
Sbjct: 301 LVFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGAL 360

Query: 361 ALDLPITAQEASRGLVPPATAIALMGKGGSILLLIMLFMAVTSAGSSELISISSLCTYDI 420
           ALDLPITAQEASRGLVPPATAIALMGKGGSILLLIMLFMAVTSAGSSELISISSLCTYDI
Sbjct: 361 ALDLPITAQEASRGLVPPATAIALMGKGGSILLLIMLFMAVTSAGSSELISISSLCTYDI 420

Query: 421 YRTYVNPDASGKSILRVSRAVILVFGCLMGLLAILLNKAGVSLGWMYLAMGVLIGSAVLP 480
           YRTYVNPDASGKSILRVSRAVILVFGCLMGLLAILLNKAGVSLGWMYLAMGVLIGSAVLP
Sbjct: 421 YRTYVNPDASGKSILRVSRAVILVFGCLMGLLAILLNKAGVSLGWMYLAMGVLIGSAVLP 480

Query: 481 IAFMLLWSKANSIGAILGTTIGCVLGIVTWVSVAKIEYGNVNLDTTGRNAPMLAGNLVSI 540
           IAFMLLWSKANSIGAILGTTIGCVLGIVTWVSVAKIEYGNVNLDTTGRNAPMLAGNLVSI
Sbjct: 481 IAFMLLWSKANSIGAILGTTIGCVLGIVTWVSVAKIEYGNVNLDTTGRNAPMLAGNLVSI 540

Query: 541 LSGGAIHVIFSLVWPQNYDWESTREISMVEKDKSELPSEEYKEEKLIRAKVWIMKWGVGF 600
           LSGGAIHVIFSLVWPQNYDWESTREISMVEKDKSELPSEEYKEEKLIRAKVWIMKWGVGF
Sbjct: 541 LSGGAIHVIFSLVWPQNYDWESTREISMVEKDKSELPSEEYKEEKLIRAKVWIMKWGVGF 600

Query: 601 TVVIVILWPLLSLPIGEFSKGYFTLWAVIAIAWGTIGSAVIIFLPLIESWETIQNVLLGM 660
           TVVIVILWPLLSLPIGEFSKGYFTLWAVIAIAWGTIGSAVIIFLPLIESWETIQNVLLGM
Sbjct: 601 TVVIVILWPLLSLPIGEFSKGYFTLWAVIAIAWGTIGSAVIIFLPLIESWETIQNVLLGM 660

Query: 661 FTNDRLVEKVEEMNLKLHALVLALPEAERIYLLEKEKARKKDLLDTDTQAHHV 713
           FTNDRLVEKVEEMNLKLHALVLALPEAERIYLLEKEKARKKDLLDTDTQAHHV
Sbjct: 661 FTNDRLVEKVEEMNLKLHALVLALPEAERIYLLEKEKARKKDLLDTDTQAHHV 712

BLAST of MC10g1384 vs. NCBI nr
Match: XP_038876583.1 (urea-proton symporter DUR3 [Benincasa hispida] >XP_038876584.1 urea-proton symporter DUR3 [Benincasa hispida])

HSP 1 Score: 1222 bits (3163), Expect = 0.0
Identity = 627/705 (88.94%), Postives = 671/705 (95.18%), Query Frame = 0

Query: 1   MAAPISKCPPFEFAGEYYSAAAAGCVRRSSFFDDRAVLDQGVGYSVILGFGAFFAVFTSF 60
           MA+   KCPPF+FA EYY  A  GCVR+SSFF+D+AVLDQG+GYSVILGFGAFFAVFTSF
Sbjct: 1   MASSALKCPPFDFAAEYYREAVGGCVRQSSFFNDKAVLDQGIGYSVILGFGAFFAVFTSF 60

Query: 61  LVWLENQYIGSRHTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGPF 120
           LVWLEN+YIGSR TSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGPF
Sbjct: 61  LVWLENRYIGSRQTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGPF 120

Query: 121 WYASGATIQASLLFGIMAIEIKRKAPNAHTVCEIVKARWGTAAHIVFLVFCFLTNIIVTA 180
           WYASGATIQ  LLFGIMAIEIKRKAPNAHTVCEIV+ARWGT AHIVFL+FCF+TNIIVTA
Sbjct: 121 WYASGATIQV-LLFGIMAIEIKRKAPNAHTVCEIVRARWGTPAHIVFLIFCFMTNIIVTA 180

Query: 181 MLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHVVLVIFV 240
           MLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHV+LVIFV
Sbjct: 181 MLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHVILVIFV 240

Query: 241 FLVYTSSSRLGSPSVVYNRLMEVASKSRICHEPISHIGQSCGPVAGNYKGSYATMLSSGG 300
           FLVY SSS+LGSPSVV++RLMEVASKSRIC EPISH GQSCGPV+GN+KGSYATMLSSGG
Sbjct: 241 FLVYVSSSQLGSPSVVFDRLMEVASKSRICQEPISHDGQSCGPVSGNFKGSYATMLSSGG 300

Query: 301 LVFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGAL 360
           LVFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGAL
Sbjct: 301 LVFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGAL 360

Query: 361 ALDLPITAQEASRGLVPPATAIALMGKGGSILLLIMLFMAVTSAGSSELISISSLCTYDI 420
           ALDLPITA EASRGLVPPATAIALMGKGGS+LLL+MLFMAVTSAGSSELIS+SSLCTYDI
Sbjct: 361 ALDLPITADEASRGLVPPATAIALMGKGGSVLLLVMLFMAVTSAGSSELISVSSLCTYDI 420

Query: 421 YRTYVNPDASGKSILRVSRAVILVFGCLMGLLAILLNKAGVSLGWMYLAMGVLIGSAVLP 480
           YRTYVNP+ASGKSIL+VSRAVILVFGC MGLLAI+LNKAGVSLGWMYLAMGVLIGSAV+P
Sbjct: 421 YRTYVNPNASGKSILKVSRAVILVFGCFMGLLAIVLNKAGVSLGWMYLAMGVLIGSAVIP 480

Query: 481 IAFMLLWSKANSIGAILGTTIGCVLGIVTWVSVAKIEYGNVNLDTTGRNAPMLAGNLVSI 540
           IAFMLLWSKANS GAILGTTIGCVLG+VTWVSVAKIEYG VNLDTTG+NAPMLAGNLVSI
Sbjct: 481 IAFMLLWSKANSKGAILGTTIGCVLGVVTWVSVAKIEYGKVNLDTTGKNAPMLAGNLVSI 540

Query: 541 LSGGAIHVIFSLVWPQNYDWESTREISMVEKDKSELPSEEYKEEKLIRAKVWIMKWGVGF 600
           LSGG IH I SL+ PQNYDWE+TR IS VEKDK ++P+EEY E KLI+AK WIMKWG+GF
Sbjct: 541 LSGGVIHAISSLLSPQNYDWETTRAISTVEKDKGDIPTEEYTEAKLIKAKAWIMKWGIGF 600

Query: 601 TVVIVILWPLLSLPIGEFSKGYFTLWAVIAIAWGTIGSAVIIFLPLIESWETIQNVLLGM 660
           T+VIV+LWP+ SLPIGEF+K YFTLWA+I+IAWGTIGS VIIFLPL+ESWETI++V LGM
Sbjct: 601 TIVIVVLWPVFSLPIGEFNKKYFTLWAIISIAWGTIGSVVIIFLPLVESWETIRDVTLGM 660

Query: 661 FTNDRLVEKVEEMNLKLHALVLALPEAERIYLLEKEKARKKDLLD 705
           FTNDRL+EKV+EMNLKLHALV+ALPEAERIYLLEKEKARKK LLD
Sbjct: 661 FTNDRLIEKVDEMNLKLHALVMALPEAERIYLLEKEKARKKGLLD 704

BLAST of MC10g1384 vs. NCBI nr
Match: XP_008448582.1 (PREDICTED: urea-proton symporter DUR3 [Cucumis melo] >KAA0052983.1 urea-proton symporter DUR3 [Cucumis melo var. makuwa])

HSP 1 Score: 1213 bits (3139), Expect = 0.0
Identity = 621/706 (87.96%), Postives = 672/706 (95.18%), Query Frame = 0

Query: 1   MAAPISKCPPFEFAGEYYSAAAAG-CVRRSSFFDDRAVLDQGVGYSVILGFGAFFAVFTS 60
           MA+   +CPPF+F+ EYY AA AG CVR+SSFF+D+AVLDQG+GYSVILGFGAFFA FTS
Sbjct: 1   MASSAIRCPPFDFSAEYYEAAGAGRCVRQSSFFNDKAVLDQGIGYSVILGFGAFFAFFTS 60

Query: 61  FLVWLENQYIGSRHTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGP 120
           FLVWLEN+YIGSR TSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGP
Sbjct: 61  FLVWLENRYIGSRQTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGP 120

Query: 121 FWYASGATIQASLLFGIMAIEIKRKAPNAHTVCEIVKARWGTAAHIVFLVFCFLTNIIVT 180
           FWYASGATIQ  LLFGIMAIEIKRKAPNAHTVCEIV+ARWGT AHIVFL+FCF+TNIIVT
Sbjct: 121 FWYASGATIQV-LLFGIMAIEIKRKAPNAHTVCEIVRARWGTPAHIVFLIFCFMTNIIVT 180

Query: 181 AMLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHVVLVIF 240
           AMLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHV+LVIF
Sbjct: 181 AMLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHVILVIF 240

Query: 241 VFLVYTSSSRLGSPSVVYNRLMEVASKSRICHEPISHIGQSCGPVAGNYKGSYATMLSSG 300
           VFLVY SSS+LGSPSVV++RLMEVASKSRIC EPISH GQSCGPV+GN+KGSYATMLSSG
Sbjct: 241 VFLVYVSSSQLGSPSVVFDRLMEVASKSRICQEPISHDGQSCGPVSGNFKGSYATMLSSG 300

Query: 301 GLVFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGA 360
           GL+FGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGA
Sbjct: 301 GLIFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGA 360

Query: 361 LALDLPITAQEASRGLVPPATAIALMGKGGSILLLIMLFMAVTSAGSSELISISSLCTYD 420
           LALDLPIT +EASRGLVPPATAIALMGKGGSILLL+MLFMAVTSAGSSELIS+SSLCTYD
Sbjct: 361 LALDLPITTEEASRGLVPPATAIALMGKGGSILLLVMLFMAVTSAGSSELISVSSLCTYD 420

Query: 421 IYRTYVNPDASGKSILRVSRAVILVFGCLMGLLAILLNKAGVSLGWMYLAMGVLIGSAVL 480
           IYRTYVNP+ASGKSIL+VSRAVILVFGC MGLLAI+LNKAGVSLGWMYLAMGV IGSAV+
Sbjct: 421 IYRTYVNPNASGKSILKVSRAVILVFGCFMGLLAIVLNKAGVSLGWMYLAMGVFIGSAVI 480

Query: 481 PIAFMLLWSKANSIGAILGTTIGCVLGIVTWVSVAKIEYGNVNLDTTGRNAPMLAGNLVS 540
           PIAFMLLW KANS GAILGTT+GCVLG+VTWVSVAKI+YG VNLDTTG+NAPMLAGNLVS
Sbjct: 481 PIAFMLLWKKANSKGAILGTTVGCVLGVVTWVSVAKIKYGEVNLDTTGKNAPMLAGNLVS 540

Query: 541 ILSGGAIHVIFSLVWPQNYDWESTREISMVEKDKSELPSEEYKEEKLIRAKVWIMKWGVG 600
           ILSGG IH I SL+ PQNYDWE+TR IS VEKDK ++P+EEY E KLI+AK WIM+WG+G
Sbjct: 541 ILSGGVIHAISSLLAPQNYDWETTRAISTVEKDKGDIPTEEYTEAKLIKAKAWIMRWGIG 600

Query: 601 FTVVIVILWPLLSLPIGEFSKGYFTLWAVIAIAWGTIGSAVIIFLPLIESWETIQNVLLG 660
           FT+VIV+LWP+LSLPIGEF+K YFTLWA+I+IAWGTIGS VIIFLPLIESWET+++V+LG
Sbjct: 601 FTIVIVVLWPVLSLPIGEFNKKYFTLWAIISIAWGTIGSVVIIFLPLIESWETLRDVMLG 660

Query: 661 MFTNDRLVEKVEEMNLKLHALVLALPEAERIYLLEKEKARKKDLLD 705
           MFTNDRL+EKV EMNLKLHALV+ALPEAERIYLLEKEKARKKDLL+
Sbjct: 661 MFTNDRLIEKVNEMNLKLHALVMALPEAERIYLLEKEKARKKDLLE 705

BLAST of MC10g1384 vs. NCBI nr
Match: XP_011650307.1 (urea-proton symporter DUR3 [Cucumis sativus] >KGN55684.1 hypothetical protein Csa_011043 [Cucumis sativus])

HSP 1 Score: 1211 bits (3134), Expect = 0.0
Identity = 622/706 (88.10%), Postives = 668/706 (94.62%), Query Frame = 0

Query: 1   MAAPISKCPPFEFAGEYYSAAAAG-CVRRSSFFDDRAVLDQGVGYSVILGFGAFFAVFTS 60
           MA+   KCPPF+F+ EYY AA AG C+R+SSFF+D+AVLDQG+GYSVILGFGAFFA FTS
Sbjct: 1   MASSALKCPPFDFSAEYYEAAGAGRCIRQSSFFNDKAVLDQGIGYSVILGFGAFFAFFTS 60

Query: 61  FLVWLENQYIGSRHTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGP 120
           FLVWLEN+YIGSR TSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGP
Sbjct: 61  FLVWLENRYIGSRQTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGP 120

Query: 121 FWYASGATIQASLLFGIMAIEIKRKAPNAHTVCEIVKARWGTAAHIVFLVFCFLTNIIVT 180
           FWYASGATIQ  LLFGIMAIEIKRKAPNAHTVCEIV+ARWGT AHIVFL+FCF+TNIIVT
Sbjct: 121 FWYASGATIQV-LLFGIMAIEIKRKAPNAHTVCEIVRARWGTPAHIVFLIFCFMTNIIVT 180

Query: 181 AMLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHVVLVIF 240
           AMLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHV+LVIF
Sbjct: 181 AMLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHVILVIF 240

Query: 241 VFLVYTSSSRLGSPSVVYNRLMEVASKSRICHEPISHIGQSCGPVAGNYKGSYATMLSSG 300
           VFLVY SSS+LGSPSVV++RLMEVASKSRIC EPISH GQSCGPV+GN+KGSYATMLSSG
Sbjct: 241 VFLVYVSSSQLGSPSVVFDRLMEVASKSRICQEPISHDGQSCGPVSGNFKGSYATMLSSG 300

Query: 301 GLVFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGA 360
           GLVFGIINI+GNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGA
Sbjct: 301 GLVFGIINIIGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGA 360

Query: 361 LALDLPITAQEASRGLVPPATAIALMGKGGSILLLIMLFMAVTSAGSSELISISSLCTYD 420
           LALDLPIT +EASRGLVPPATAIALMGKGGSILLL+MLFMAVTSAGSSELIS+SSLCTYD
Sbjct: 361 LALDLPITTEEASRGLVPPATAIALMGKGGSILLLVMLFMAVTSAGSSELISVSSLCTYD 420

Query: 421 IYRTYVNPDASGKSILRVSRAVILVFGCLMGLLAILLNKAGVSLGWMYLAMGVLIGSAVL 480
           IYRTYVNP+ASGKSIL+VSRAVILVFGC MGLLAI+LNKAGVSLGWMYLAMGV IGSAV+
Sbjct: 421 IYRTYVNPNASGKSILKVSRAVILVFGCFMGLLAIVLNKAGVSLGWMYLAMGVFIGSAVI 480

Query: 481 PIAFMLLWSKANSIGAILGTTIGCVLGIVTWVSVAKIEYGNVNLDTTGRNAPMLAGNLVS 540
           PIAFMLLW KANS GAILGTTIGCVLG+VTWVS  KI+YG VNLDTTGRNAPMLAGNLVS
Sbjct: 481 PIAFMLLWKKANSKGAILGTTIGCVLGVVTWVSATKIKYGEVNLDTTGRNAPMLAGNLVS 540

Query: 541 ILSGGAIHVIFSLVWPQNYDWESTREISMVEKDKSELPSEEYKEEKLIRAKVWIMKWGVG 600
           ILSGG IH I SL+ PQNYDWE+TR IS VEKD  ++P+EEY E KLIRAK WIMKWG+G
Sbjct: 541 ILSGGVIHAISSLLEPQNYDWETTRAISTVEKDNGDIPTEEYTEAKLIRAKAWIMKWGIG 600

Query: 601 FTVVIVILWPLLSLPIGEFSKGYFTLWAVIAIAWGTIGSAVIIFLPLIESWETIQNVLLG 660
           FT+VIV+LWP+LSLPIGEF+K YFTLWA+I+IAWGTIGSAVIIFLPLIESWET+++V LG
Sbjct: 601 FTIVIVVLWPVLSLPIGEFNKKYFTLWAIISIAWGTIGSAVIIFLPLIESWETLRDVTLG 660

Query: 661 MFTNDRLVEKVEEMNLKLHALVLALPEAERIYLLEKEKARKKDLLD 705
           MFTNDRL+EKV EMNLKLHALV+ALPEAERIYLLEKE ARKKDLL+
Sbjct: 661 MFTNDRLIEKVNEMNLKLHALVMALPEAERIYLLEKENARKKDLLE 705

BLAST of MC10g1384 vs. NCBI nr
Match: XP_023005805.1 (LOW QUALITY PROTEIN: urea-proton symporter DUR3 [Cucurbita maxima])

HSP 1 Score: 1189 bits (3076), Expect = 0.0
Identity = 614/711 (86.36%), Postives = 671/711 (94.37%), Query Frame = 0

Query: 1   MAAPISKCPPFEFAGEYYSAAAAGCVRRSSFFDDRAVLDQGVGYSVILGFGAFFAVFTSF 60
           MAA + KCPPF+FA +YY A AAGCVR+SSFF+ +AVLDQG+GYSVILGFGAFFAVFTSF
Sbjct: 1   MAASVPKCPPFDFADKYYQATAAGCVRQSSFFNGQAVLDQGIGYSVILGFGAFFAVFTSF 60

Query: 61  LVWLENQYIGSRHTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGPF 120
           LVWLEN+YIGS+ TSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGPF
Sbjct: 61  LVWLENRYIGSKKTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGPF 120

Query: 121 WYASGATIQASLLFGIMAIEIKRKAPNAHTVCEIVKARWGTAAHIVFLVFCFLTNIIVTA 180
           WYASGATIQ  LLFGIMAIEIKRKAPNAHTVCEIV+ARWGT AHIVFLVFCF+TNI+VTA
Sbjct: 121 WYASGATIQV-LLFGIMAIEIKRKAPNAHTVCEIVRARWGTPAHIVFLVFCFMTNILVTA 180

Query: 181 MLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHVVLVIFV 240
           MLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHV+LVIFV
Sbjct: 181 MLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHVILVIFV 240

Query: 241 FLVYTSSSRLGSPSVVYNRLMEVASKSRICHEPISHIGQSCGPVAGNYKGSYATMLSSGG 300
           FLVYTSSS+LGSPSVVY+ LMEVASK+R+C EPISH GQSCGPV+GN+KGSYAT+LSSGG
Sbjct: 241 FLVYTSSSQLGSPSVVYDHLMEVASKARMCQEPISHDGQSCGPVSGNFKGSYATLLSSGG 300

Query: 301 LVFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGAL 360
           LVFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGAL
Sbjct: 301 LVFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGAL 360

Query: 361 ALDLPITAQEASRGLVPPATAIALMGKGGSILLLIMLFMAVTSAGSSELISISSLCTYDI 420
           ALDLPIT  EASRGLVPPATAIALMGKGGS+LLL+MLFMAVTSAGSSELIS+SSLCTYDI
Sbjct: 361 ALDLPITTDEASRGLVPPATAIALMGKGGSVLLLVMLFMAVTSAGSSELISVSSLCTYDI 420

Query: 421 YRTYVNPDASGKSILRVSRAVILVFGCLMGLLAILLNKAGVSLGWMYLAMGVLIGSAVLP 480
           YRTYVNP+ASGKSIL+VSRAVILVFGC MGLLAI+LNKAGVSLGWMYLAMGV IGSAV+P
Sbjct: 421 YRTYVNPNASGKSILKVSRAVILVFGCFMGLLAIVLNKAGVSLGWMYLAMGVFIGSAVIP 480

Query: 481 IAFMLLWSKANSIGAILGTTIGCVLGIVTWVSVAKIEYGNVNLDTTGRNAPMLAGNLVSI 540
           IAFMLLW+KANS GAILGTTIGCVLG++TW+SV KIEYG VNL+TTGRNAPMLAGNLVSI
Sbjct: 481 IAFMLLWTKANSNGAILGTTIGCVLGVITWISVTKIEYGEVNLNTTGRNAPMLAGNLVSI 540

Query: 541 LSGGAIHVIFSLVWPQNYDWESTREISMVEKDKSELPSEEYKEEKLIRAKVWIMKWGVGF 600
           LSGG IH I SL+ PQNYDWE+TR+IS VEKD  E+PSEEYKEEKL++AK WI+KWGVGF
Sbjct: 541 LSGGIIHAISSLLSPQNYDWETTRKISTVEKDNGEIPSEEYKEEKLMKAKAWIVKWGVGF 600

Query: 601 TVVIVILWPLLSLPIGEFSKGYFTLWAVIAIAWGTIGSAVIIFLPLIESWETIQNVLLGM 660
           T+VIVILWPLLSLPIGEF+K YFTLWAVIAIAWGTIGSAVIIFLPLIES ET++ V+LGM
Sbjct: 601 TIVIVILWPLLSLPIGEFNKKYFTLWAVIAIAWGTIGSAVIIFLPLIESRETLRAVMLGM 660

Query: 661 FTNDRLVEKVEEMNLKLHALVLALPEAERIYLLEKEK-ARKKDLLDTDTQA 710
           FT+D+L + ++EM+LKL++ V+A+PE+ERI LLEK+K ARK+DLLD    A
Sbjct: 661 FTSDKLGDTMDEMSLKLYSSVMAVPESERIXLLEKKKNARKEDLLDIPDHA 710

BLAST of MC10g1384 vs. ExPASy TrEMBL
Match: A0A6J1CV76 (urea-proton symporter DUR3 OS=Momordica charantia OX=3673 GN=LOC111014871 PE=3 SV=1)

HSP 1 Score: 1357 bits (3513), Expect = 0.0
Identity = 711/713 (99.72%), Postives = 711/713 (99.72%), Query Frame = 0

Query: 1   MAAPISKCPPFEFAGEYYSAAAAGCVRRSSFFDDRAVLDQGVGYSVILGFGAFFAVFTSF 60
           MAAPISKCPPFEFAGEYYSAAAAGCVRRSSFFDDRAVLDQGVGYSVILGFGAFFAVFTSF
Sbjct: 1   MAAPISKCPPFEFAGEYYSAAAAGCVRRSSFFDDRAVLDQGVGYSVILGFGAFFAVFTSF 60

Query: 61  LVWLENQYIGSRHTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGPF 120
           LVWLENQYIGSRHTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGPF
Sbjct: 61  LVWLENQYIGSRHTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGPF 120

Query: 121 WYASGATIQASLLFGIMAIEIKRKAPNAHTVCEIVKARWGTAAHIVFLVFCFLTNIIVTA 180
           WYASGATIQ  LLFGIMAIEIKRKAPNAHTVCEIVKARWGTAAHIVFLVFCFLTNIIVTA
Sbjct: 121 WYASGATIQV-LLFGIMAIEIKRKAPNAHTVCEIVKARWGTAAHIVFLVFCFLTNIIVTA 180

Query: 181 MLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHVVLVIFV 240
           MLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHVVLVIFV
Sbjct: 181 MLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHVVLVIFV 240

Query: 241 FLVYTSSSRLGSPSVVYNRLMEVASKSRICHEPISHIGQSCGPVAGNYKGSYATMLSSGG 300
           FLVYTSSSRLGSPSVVYNRLMEVASKSRICHEPISHIGQSCGPVAGNYKGSYATMLSSGG
Sbjct: 241 FLVYTSSSRLGSPSVVYNRLMEVASKSRICHEPISHIGQSCGPVAGNYKGSYATMLSSGG 300

Query: 301 LVFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGAL 360
           LVFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGAL
Sbjct: 301 LVFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGAL 360

Query: 361 ALDLPITAQEASRGLVPPATAIALMGKGGSILLLIMLFMAVTSAGSSELISISSLCTYDI 420
           ALDLPITAQEASRGLVPPATAIALMGKGGSILLLIMLFMAVTSAGSSELISISSLCTYDI
Sbjct: 361 ALDLPITAQEASRGLVPPATAIALMGKGGSILLLIMLFMAVTSAGSSELISISSLCTYDI 420

Query: 421 YRTYVNPDASGKSILRVSRAVILVFGCLMGLLAILLNKAGVSLGWMYLAMGVLIGSAVLP 480
           YRTYVNPDASGKSILRVSRAVILVFGCLMGLLAILLNKAGVSLGWMYLAMGVLIGSAVLP
Sbjct: 421 YRTYVNPDASGKSILRVSRAVILVFGCLMGLLAILLNKAGVSLGWMYLAMGVLIGSAVLP 480

Query: 481 IAFMLLWSKANSIGAILGTTIGCVLGIVTWVSVAKIEYGNVNLDTTGRNAPMLAGNLVSI 540
           IAFMLLWSKANSIGAILGTTIGCVLGIVTWVSVAKIEYGNVNLDTTGRNAPMLAGNLVSI
Sbjct: 481 IAFMLLWSKANSIGAILGTTIGCVLGIVTWVSVAKIEYGNVNLDTTGRNAPMLAGNLVSI 540

Query: 541 LSGGAIHVIFSLVWPQNYDWESTREISMVEKDKSELPSEEYKEEKLIRAKVWIMKWGVGF 600
           LSGGAIHVIFSLVWPQNYDWESTREISMVEKDKSELPSEEYKEEKLIRAKVWIMKWGVGF
Sbjct: 541 LSGGAIHVIFSLVWPQNYDWESTREISMVEKDKSELPSEEYKEEKLIRAKVWIMKWGVGF 600

Query: 601 TVVIVILWPLLSLPIGEFSKGYFTLWAVIAIAWGTIGSAVIIFLPLIESWETIQNVLLGM 660
           TVVIVILWPLLSLPIGEFSKGYFTLWAVIAIAWGTIGSAVIIFLPLIESWETIQNVLLGM
Sbjct: 601 TVVIVILWPLLSLPIGEFSKGYFTLWAVIAIAWGTIGSAVIIFLPLIESWETIQNVLLGM 660

Query: 661 FTNDRLVEKVEEMNLKLHALVLALPEAERIYLLEKEKARKKDLLDTDTQAHHV 713
           FTNDRLVEKVEEMNLKLHALVLALPEAERIYLLEKEKARKKDLLDTDTQAHHV
Sbjct: 661 FTNDRLVEKVEEMNLKLHALVLALPEAERIYLLEKEKARKKDLLDTDTQAHHV 712

BLAST of MC10g1384 vs. ExPASy TrEMBL
Match: A0A5A7UAL4 (Urea-proton symporter DUR3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold344G00940 PE=3 SV=1)

HSP 1 Score: 1213 bits (3139), Expect = 0.0
Identity = 621/706 (87.96%), Postives = 672/706 (95.18%), Query Frame = 0

Query: 1   MAAPISKCPPFEFAGEYYSAAAAG-CVRRSSFFDDRAVLDQGVGYSVILGFGAFFAVFTS 60
           MA+   +CPPF+F+ EYY AA AG CVR+SSFF+D+AVLDQG+GYSVILGFGAFFA FTS
Sbjct: 1   MASSAIRCPPFDFSAEYYEAAGAGRCVRQSSFFNDKAVLDQGIGYSVILGFGAFFAFFTS 60

Query: 61  FLVWLENQYIGSRHTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGP 120
           FLVWLEN+YIGSR TSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGP
Sbjct: 61  FLVWLENRYIGSRQTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGP 120

Query: 121 FWYASGATIQASLLFGIMAIEIKRKAPNAHTVCEIVKARWGTAAHIVFLVFCFLTNIIVT 180
           FWYASGATIQ  LLFGIMAIEIKRKAPNAHTVCEIV+ARWGT AHIVFL+FCF+TNIIVT
Sbjct: 121 FWYASGATIQV-LLFGIMAIEIKRKAPNAHTVCEIVRARWGTPAHIVFLIFCFMTNIIVT 180

Query: 181 AMLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHVVLVIF 240
           AMLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHV+LVIF
Sbjct: 181 AMLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHVILVIF 240

Query: 241 VFLVYTSSSRLGSPSVVYNRLMEVASKSRICHEPISHIGQSCGPVAGNYKGSYATMLSSG 300
           VFLVY SSS+LGSPSVV++RLMEVASKSRIC EPISH GQSCGPV+GN+KGSYATMLSSG
Sbjct: 241 VFLVYVSSSQLGSPSVVFDRLMEVASKSRICQEPISHDGQSCGPVSGNFKGSYATMLSSG 300

Query: 301 GLVFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGA 360
           GL+FGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGA
Sbjct: 301 GLIFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGA 360

Query: 361 LALDLPITAQEASRGLVPPATAIALMGKGGSILLLIMLFMAVTSAGSSELISISSLCTYD 420
           LALDLPIT +EASRGLVPPATAIALMGKGGSILLL+MLFMAVTSAGSSELIS+SSLCTYD
Sbjct: 361 LALDLPITTEEASRGLVPPATAIALMGKGGSILLLVMLFMAVTSAGSSELISVSSLCTYD 420

Query: 421 IYRTYVNPDASGKSILRVSRAVILVFGCLMGLLAILLNKAGVSLGWMYLAMGVLIGSAVL 480
           IYRTYVNP+ASGKSIL+VSRAVILVFGC MGLLAI+LNKAGVSLGWMYLAMGV IGSAV+
Sbjct: 421 IYRTYVNPNASGKSILKVSRAVILVFGCFMGLLAIVLNKAGVSLGWMYLAMGVFIGSAVI 480

Query: 481 PIAFMLLWSKANSIGAILGTTIGCVLGIVTWVSVAKIEYGNVNLDTTGRNAPMLAGNLVS 540
           PIAFMLLW KANS GAILGTT+GCVLG+VTWVSVAKI+YG VNLDTTG+NAPMLAGNLVS
Sbjct: 481 PIAFMLLWKKANSKGAILGTTVGCVLGVVTWVSVAKIKYGEVNLDTTGKNAPMLAGNLVS 540

Query: 541 ILSGGAIHVIFSLVWPQNYDWESTREISMVEKDKSELPSEEYKEEKLIRAKVWIMKWGVG 600
           ILSGG IH I SL+ PQNYDWE+TR IS VEKDK ++P+EEY E KLI+AK WIM+WG+G
Sbjct: 541 ILSGGVIHAISSLLAPQNYDWETTRAISTVEKDKGDIPTEEYTEAKLIKAKAWIMRWGIG 600

Query: 601 FTVVIVILWPLLSLPIGEFSKGYFTLWAVIAIAWGTIGSAVIIFLPLIESWETIQNVLLG 660
           FT+VIV+LWP+LSLPIGEF+K YFTLWA+I+IAWGTIGS VIIFLPLIESWET+++V+LG
Sbjct: 601 FTIVIVVLWPVLSLPIGEFNKKYFTLWAIISIAWGTIGSVVIIFLPLIESWETLRDVMLG 660

Query: 661 MFTNDRLVEKVEEMNLKLHALVLALPEAERIYLLEKEKARKKDLLD 705
           MFTNDRL+EKV EMNLKLHALV+ALPEAERIYLLEKEKARKKDLL+
Sbjct: 661 MFTNDRLIEKVNEMNLKLHALVMALPEAERIYLLEKEKARKKDLLE 705

BLAST of MC10g1384 vs. ExPASy TrEMBL
Match: A0A1S3BKN1 (urea-proton symporter DUR3 OS=Cucumis melo OX=3656 GN=LOC103490716 PE=3 SV=1)

HSP 1 Score: 1213 bits (3139), Expect = 0.0
Identity = 621/706 (87.96%), Postives = 672/706 (95.18%), Query Frame = 0

Query: 1   MAAPISKCPPFEFAGEYYSAAAAG-CVRRSSFFDDRAVLDQGVGYSVILGFGAFFAVFTS 60
           MA+   +CPPF+F+ EYY AA AG CVR+SSFF+D+AVLDQG+GYSVILGFGAFFA FTS
Sbjct: 1   MASSAIRCPPFDFSAEYYEAAGAGRCVRQSSFFNDKAVLDQGIGYSVILGFGAFFAFFTS 60

Query: 61  FLVWLENQYIGSRHTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGP 120
           FLVWLEN+YIGSR TSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGP
Sbjct: 61  FLVWLENRYIGSRQTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGP 120

Query: 121 FWYASGATIQASLLFGIMAIEIKRKAPNAHTVCEIVKARWGTAAHIVFLVFCFLTNIIVT 180
           FWYASGATIQ  LLFGIMAIEIKRKAPNAHTVCEIV+ARWGT AHIVFL+FCF+TNIIVT
Sbjct: 121 FWYASGATIQV-LLFGIMAIEIKRKAPNAHTVCEIVRARWGTPAHIVFLIFCFMTNIIVT 180

Query: 181 AMLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHVVLVIF 240
           AMLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHV+LVIF
Sbjct: 181 AMLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHVILVIF 240

Query: 241 VFLVYTSSSRLGSPSVVYNRLMEVASKSRICHEPISHIGQSCGPVAGNYKGSYATMLSSG 300
           VFLVY SSS+LGSPSVV++RLMEVASKSRIC EPISH GQSCGPV+GN+KGSYATMLSSG
Sbjct: 241 VFLVYVSSSQLGSPSVVFDRLMEVASKSRICQEPISHDGQSCGPVSGNFKGSYATMLSSG 300

Query: 301 GLVFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGA 360
           GL+FGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGA
Sbjct: 301 GLIFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGA 360

Query: 361 LALDLPITAQEASRGLVPPATAIALMGKGGSILLLIMLFMAVTSAGSSELISISSLCTYD 420
           LALDLPIT +EASRGLVPPATAIALMGKGGSILLL+MLFMAVTSAGSSELIS+SSLCTYD
Sbjct: 361 LALDLPITTEEASRGLVPPATAIALMGKGGSILLLVMLFMAVTSAGSSELISVSSLCTYD 420

Query: 421 IYRTYVNPDASGKSILRVSRAVILVFGCLMGLLAILLNKAGVSLGWMYLAMGVLIGSAVL 480
           IYRTYVNP+ASGKSIL+VSRAVILVFGC MGLLAI+LNKAGVSLGWMYLAMGV IGSAV+
Sbjct: 421 IYRTYVNPNASGKSILKVSRAVILVFGCFMGLLAIVLNKAGVSLGWMYLAMGVFIGSAVI 480

Query: 481 PIAFMLLWSKANSIGAILGTTIGCVLGIVTWVSVAKIEYGNVNLDTTGRNAPMLAGNLVS 540
           PIAFMLLW KANS GAILGTT+GCVLG+VTWVSVAKI+YG VNLDTTG+NAPMLAGNLVS
Sbjct: 481 PIAFMLLWKKANSKGAILGTTVGCVLGVVTWVSVAKIKYGEVNLDTTGKNAPMLAGNLVS 540

Query: 541 ILSGGAIHVIFSLVWPQNYDWESTREISMVEKDKSELPSEEYKEEKLIRAKVWIMKWGVG 600
           ILSGG IH I SL+ PQNYDWE+TR IS VEKDK ++P+EEY E KLI+AK WIM+WG+G
Sbjct: 541 ILSGGVIHAISSLLAPQNYDWETTRAISTVEKDKGDIPTEEYTEAKLIKAKAWIMRWGIG 600

Query: 601 FTVVIVILWPLLSLPIGEFSKGYFTLWAVIAIAWGTIGSAVIIFLPLIESWETIQNVLLG 660
           FT+VIV+LWP+LSLPIGEF+K YFTLWA+I+IAWGTIGS VIIFLPLIESWET+++V+LG
Sbjct: 601 FTIVIVVLWPVLSLPIGEFNKKYFTLWAIISIAWGTIGSVVIIFLPLIESWETLRDVMLG 660

Query: 661 MFTNDRLVEKVEEMNLKLHALVLALPEAERIYLLEKEKARKKDLLD 705
           MFTNDRL+EKV EMNLKLHALV+ALPEAERIYLLEKEKARKKDLL+
Sbjct: 661 MFTNDRLIEKVNEMNLKLHALVMALPEAERIYLLEKEKARKKDLLE 705

BLAST of MC10g1384 vs. ExPASy TrEMBL
Match: A0A0A0L3R6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G004500 PE=3 SV=1)

HSP 1 Score: 1211 bits (3134), Expect = 0.0
Identity = 622/706 (88.10%), Postives = 668/706 (94.62%), Query Frame = 0

Query: 1   MAAPISKCPPFEFAGEYYSAAAAG-CVRRSSFFDDRAVLDQGVGYSVILGFGAFFAVFTS 60
           MA+   KCPPF+F+ EYY AA AG C+R+SSFF+D+AVLDQG+GYSVILGFGAFFA FTS
Sbjct: 1   MASSALKCPPFDFSAEYYEAAGAGRCIRQSSFFNDKAVLDQGIGYSVILGFGAFFAFFTS 60

Query: 61  FLVWLENQYIGSRHTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGP 120
           FLVWLEN+YIGSR TSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGP
Sbjct: 61  FLVWLENRYIGSRQTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGP 120

Query: 121 FWYASGATIQASLLFGIMAIEIKRKAPNAHTVCEIVKARWGTAAHIVFLVFCFLTNIIVT 180
           FWYASGATIQ  LLFGIMAIEIKRKAPNAHTVCEIV+ARWGT AHIVFL+FCF+TNIIVT
Sbjct: 121 FWYASGATIQV-LLFGIMAIEIKRKAPNAHTVCEIVRARWGTPAHIVFLIFCFMTNIIVT 180

Query: 181 AMLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHVVLVIF 240
           AMLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHV+LVIF
Sbjct: 181 AMLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHVILVIF 240

Query: 241 VFLVYTSSSRLGSPSVVYNRLMEVASKSRICHEPISHIGQSCGPVAGNYKGSYATMLSSG 300
           VFLVY SSS+LGSPSVV++RLMEVASKSRIC EPISH GQSCGPV+GN+KGSYATMLSSG
Sbjct: 241 VFLVYVSSSQLGSPSVVFDRLMEVASKSRICQEPISHDGQSCGPVSGNFKGSYATMLSSG 300

Query: 301 GLVFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGA 360
           GLVFGIINI+GNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGA
Sbjct: 301 GLVFGIINIIGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGA 360

Query: 361 LALDLPITAQEASRGLVPPATAIALMGKGGSILLLIMLFMAVTSAGSSELISISSLCTYD 420
           LALDLPIT +EASRGLVPPATAIALMGKGGSILLL+MLFMAVTSAGSSELIS+SSLCTYD
Sbjct: 361 LALDLPITTEEASRGLVPPATAIALMGKGGSILLLVMLFMAVTSAGSSELISVSSLCTYD 420

Query: 421 IYRTYVNPDASGKSILRVSRAVILVFGCLMGLLAILLNKAGVSLGWMYLAMGVLIGSAVL 480
           IYRTYVNP+ASGKSIL+VSRAVILVFGC MGLLAI+LNKAGVSLGWMYLAMGV IGSAV+
Sbjct: 421 IYRTYVNPNASGKSILKVSRAVILVFGCFMGLLAIVLNKAGVSLGWMYLAMGVFIGSAVI 480

Query: 481 PIAFMLLWSKANSIGAILGTTIGCVLGIVTWVSVAKIEYGNVNLDTTGRNAPMLAGNLVS 540
           PIAFMLLW KANS GAILGTTIGCVLG+VTWVS  KI+YG VNLDTTGRNAPMLAGNLVS
Sbjct: 481 PIAFMLLWKKANSKGAILGTTIGCVLGVVTWVSATKIKYGEVNLDTTGRNAPMLAGNLVS 540

Query: 541 ILSGGAIHVIFSLVWPQNYDWESTREISMVEKDKSELPSEEYKEEKLIRAKVWIMKWGVG 600
           ILSGG IH I SL+ PQNYDWE+TR IS VEKD  ++P+EEY E KLIRAK WIMKWG+G
Sbjct: 541 ILSGGVIHAISSLLEPQNYDWETTRAISTVEKDNGDIPTEEYTEAKLIRAKAWIMKWGIG 600

Query: 601 FTVVIVILWPLLSLPIGEFSKGYFTLWAVIAIAWGTIGSAVIIFLPLIESWETIQNVLLG 660
           FT+VIV+LWP+LSLPIGEF+K YFTLWA+I+IAWGTIGSAVIIFLPLIESWET+++V LG
Sbjct: 601 FTIVIVVLWPVLSLPIGEFNKKYFTLWAIISIAWGTIGSAVIIFLPLIESWETLRDVTLG 660

Query: 661 MFTNDRLVEKVEEMNLKLHALVLALPEAERIYLLEKEKARKKDLLD 705
           MFTNDRL+EKV EMNLKLHALV+ALPEAERIYLLEKE ARKKDLL+
Sbjct: 661 MFTNDRLIEKVNEMNLKLHALVMALPEAERIYLLEKENARKKDLLE 705

BLAST of MC10g1384 vs. ExPASy TrEMBL
Match: A0A6J1KYE3 (LOW QUALITY PROTEIN: urea-proton symporter DUR3 OS=Cucurbita maxima OX=3661 GN=LOC111498699 PE=3 SV=1)

HSP 1 Score: 1189 bits (3076), Expect = 0.0
Identity = 614/711 (86.36%), Postives = 671/711 (94.37%), Query Frame = 0

Query: 1   MAAPISKCPPFEFAGEYYSAAAAGCVRRSSFFDDRAVLDQGVGYSVILGFGAFFAVFTSF 60
           MAA + KCPPF+FA +YY A AAGCVR+SSFF+ +AVLDQG+GYSVILGFGAFFAVFTSF
Sbjct: 1   MAASVPKCPPFDFADKYYQATAAGCVRQSSFFNGQAVLDQGIGYSVILGFGAFFAVFTSF 60

Query: 61  LVWLENQYIGSRHTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGPF 120
           LVWLEN+YIGS+ TSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGPF
Sbjct: 61  LVWLENRYIGSKKTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGPF 120

Query: 121 WYASGATIQASLLFGIMAIEIKRKAPNAHTVCEIVKARWGTAAHIVFLVFCFLTNIIVTA 180
           WYASGATIQ  LLFGIMAIEIKRKAPNAHTVCEIV+ARWGT AHIVFLVFCF+TNI+VTA
Sbjct: 121 WYASGATIQV-LLFGIMAIEIKRKAPNAHTVCEIVRARWGTPAHIVFLVFCFMTNILVTA 180

Query: 181 MLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHVVLVIFV 240
           MLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHV+LVIFV
Sbjct: 181 MLLLGGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHVILVIFV 240

Query: 241 FLVYTSSSRLGSPSVVYNRLMEVASKSRICHEPISHIGQSCGPVAGNYKGSYATMLSSGG 300
           FLVYTSSS+LGSPSVVY+ LMEVASK+R+C EPISH GQSCGPV+GN+KGSYAT+LSSGG
Sbjct: 241 FLVYTSSSQLGSPSVVYDHLMEVASKARMCQEPISHDGQSCGPVSGNFKGSYATLLSSGG 300

Query: 301 LVFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGAL 360
           LVFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGAL
Sbjct: 301 LVFGIINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGAL 360

Query: 361 ALDLPITAQEASRGLVPPATAIALMGKGGSILLLIMLFMAVTSAGSSELISISSLCTYDI 420
           ALDLPIT  EASRGLVPPATAIALMGKGGS+LLL+MLFMAVTSAGSSELIS+SSLCTYDI
Sbjct: 361 ALDLPITTDEASRGLVPPATAIALMGKGGSVLLLVMLFMAVTSAGSSELISVSSLCTYDI 420

Query: 421 YRTYVNPDASGKSILRVSRAVILVFGCLMGLLAILLNKAGVSLGWMYLAMGVLIGSAVLP 480
           YRTYVNP+ASGKSIL+VSRAVILVFGC MGLLAI+LNKAGVSLGWMYLAMGV IGSAV+P
Sbjct: 421 YRTYVNPNASGKSILKVSRAVILVFGCFMGLLAIVLNKAGVSLGWMYLAMGVFIGSAVIP 480

Query: 481 IAFMLLWSKANSIGAILGTTIGCVLGIVTWVSVAKIEYGNVNLDTTGRNAPMLAGNLVSI 540
           IAFMLLW+KANS GAILGTTIGCVLG++TW+SV KIEYG VNL+TTGRNAPMLAGNLVSI
Sbjct: 481 IAFMLLWTKANSNGAILGTTIGCVLGVITWISVTKIEYGEVNLNTTGRNAPMLAGNLVSI 540

Query: 541 LSGGAIHVIFSLVWPQNYDWESTREISMVEKDKSELPSEEYKEEKLIRAKVWIMKWGVGF 600
           LSGG IH I SL+ PQNYDWE+TR+IS VEKD  E+PSEEYKEEKL++AK WI+KWGVGF
Sbjct: 541 LSGGIIHAISSLLSPQNYDWETTRKISTVEKDNGEIPSEEYKEEKLMKAKAWIVKWGVGF 600

Query: 601 TVVIVILWPLLSLPIGEFSKGYFTLWAVIAIAWGTIGSAVIIFLPLIESWETIQNVLLGM 660
           T+VIVILWPLLSLPIGEF+K YFTLWAVIAIAWGTIGSAVIIFLPLIES ET++ V+LGM
Sbjct: 601 TIVIVILWPLLSLPIGEFNKKYFTLWAVIAIAWGTIGSAVIIFLPLIESRETLRAVMLGM 660

Query: 661 FTNDRLVEKVEEMNLKLHALVLALPEAERIYLLEKEK-ARKKDLLDTDTQA 710
           FT+D+L + ++EM+LKL++ V+A+PE+ERI LLEK+K ARK+DLLD    A
Sbjct: 661 FTSDKLGDTMDEMSLKLYSSVMAVPESERIXLLEKKKNARKEDLLDIPDHA 710

BLAST of MC10g1384 vs. TAIR 10
Match: AT5G45380.1 (solute:sodium symporters;urea transmembrane transporters )

HSP 1 Score: 1060.4 bits (2741), Expect = 6.2e-310
Identity = 533/703 (75.82%), Postives = 622/703 (88.48%), Query Frame = 0

Query: 5   ISKCPPFEFAGEYYSAAAAGCVRRSSFFDDRAVLDQGVGYSVILGFGAFFAVFTSFLVWL 64
           ++ CPPF+F+ +YY     GC R+SSFF    VLDQGVGY+VILGFGAFFAVFTSFLVWL
Sbjct: 1   MATCPPFDFSTKYYD-GDGGCQRQSSFFGGTTVLDQGVGYAVILGFGAFFAVFTSFLVWL 60

Query: 65  ENQYIGSRHTSEWFNTAGRSVKTGLIASVIVSQWTWAATILQSSNVAWEYGVSGPFWYAS 124
           E +Y+G+RHTSEWFNTAGR+VKTGLIASVIVSQWTWAATILQSSNVAW+YGVSGPFWYAS
Sbjct: 61  EKRYVGARHTSEWFNTAGRNVKTGLIASVIVSQWTWAATILQSSNVAWQYGVSGPFWYAS 120

Query: 125 GATIQASLLFGIMAIEIKRKAPNAHTVCEIVKARWGTAAHIVFLVFCFLTNIIVTAMLLL 184
           GATIQ  LLFG+MAIEIKRKAPNAHTVCEIVKARWGTA HIVFLVFC  TN++VTAMLLL
Sbjct: 121 GATIQV-LLFGVMAIEIKRKAPNAHTVCEIVKARWGTATHIVFLVFCLATNVVVTAMLLL 180

Query: 185 GGSAVVNALTGVNIFAASFLIPLGVIVYTLAGGLKATFLASYIHSVIVHVVLVIFVFLVY 244
           GGSAVVNALTGVN++AASFLIPLGV+VYTLAGGLKATFLASY+HSVIVHV LV+FVFLVY
Sbjct: 181 GGSAVVNALTGVNLYAASFLIPLGVVVYTLAGGLKATFLASYVHSVIVHVALVVFVFLVY 240

Query: 245 TSSSRLGSPSVVYNRLMEVASKSRICHEPISHIGQSCGPVAGNYKGSYATMLSSGGLVFG 304
           TSS  LGSPSVVY+RL ++ +KSR C EP+SH GQ+CGPV GN++GSY TMLSSGG VFG
Sbjct: 241 TSSKELGSPSVVYDRLKDMVAKSRSCTEPLSHHGQACGPVDGNFRGSYLTMLSSGGAVFG 300

Query: 305 IINIVGNFGTVFVDNGYWMSAIAARPSSTHKGYLVGGLVWFAVPFSLATSLGLGALALDL 364
           +INIVGNFGTVFVDNGYW+SAIAARPSSTHKGYL+GGLVWFAVPFSLATSLGLGALALDL
Sbjct: 301 LINIVGNFGTVFVDNGYWVSAIAARPSSTHKGYLLGGLVWFAVPFSLATSLGLGALALDL 360

Query: 365 PITAQEASRGLVPPATAIALMGKGGSILLLIMLFMAVTSAGSSELISISSLCTYDIYRTY 424
           PI+  EA RGLVPPATAIALMGK GS+LLL MLFMAVTSAGSSELI++SSL TYDIYRTY
Sbjct: 361 PISKDEADRGLVPPATAIALMGKSGSLLLLTMLFMAVTSAGSSELIAVSSLFTYDIYRTY 420

Query: 425 VNPDASGKSILRVSRAVILVFGCLMGLLAILLNKAGVSLGWMYLAMGVLIGSAVLPIAFM 484
           +NP A+G+ IL++SR  +L FGC MG+LA++LNKAGVSLGWMYLAMGVLIGSAV+PIAFM
Sbjct: 421 INPRATGRQILKISRCAVLGFGCFMGILAVVLNKAGVSLGWMYLAMGVLIGSAVIPIAFM 480

Query: 485 LLWSKANSIGAILGTTIGCVLGIVTWVSVAKIEYGNVNLDTTGRNAPMLAGNLVSILSGG 544
           LLWSKAN+ GAILG T GCV GI+TW++ AK +YG V+LD+TG+N PMLAGNLV+IL+GG
Sbjct: 481 LLWSKANAFGAILGATSGCVFGIITWLTTAKTQYGRVDLDSTGKNGPMLAGNLVAILTGG 540

Query: 545 AIHVIFSLVWPQNYDWESTREISMVEKDKS-----ELPSEEYKEEKLIRAKVWIMKWGVG 604
            IH + SLV PQNYDW +TREI +VE   S     ++P+EE +EEKL RAK WI+KWG+ 
Sbjct: 541 LIHAVCSLVRPQNYDWSTTREIKVVEAYASGDEDVDVPAEELREEKLRRAKAWIVKWGLV 600

Query: 605 FTVVIVILWPLLSLPIGEFSKGYFTLWAVIAIAWGTIGSAVIIFLPLIESWETIQNVLLG 664
           FT++IV++WP+LSLP   FS+GYF  WA++AIAWGTIGS VII LPL+ESW+TI++V +G
Sbjct: 601 FTILIVVIWPVLSLPARVFSRGYFWFWAIVAIAWGTIGSIVIIGLPLVESWDTIKSVCMG 660

Query: 665 MFTNDRLVEKVEEMNLKLHALVLALPEAERIYLLEKEKARKKD 703
           MFTNDR+++K++++N +L AL +A+PEAE+IYLLE EK +K D
Sbjct: 661 MFTNDRVMKKLDDLNHRLRALTMAVPEAEKIYLLELEKTKKND 701

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4KD718.7e-30975.82Urea-proton symporter DUR3 OS=Arabidopsis thaliana OX=3702 GN=DUR3 PE=1 SV=1[more]
Q7XBS02.7e-29474.64Urea-proton symporter DUR3 OS=Oryza sativa subsp. japonica OX=39947 GN=DUR3 PE=1... [more]
O944691.6e-13743.03Probable urea active transporter 1 OS=Schizosaccharomyces pombe (strain 972 / AT... [more]
P334134.7e-12138.40Urea active transporter OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c)... [more]
Q9URY62.9e-11539.87Probable urea active transporter 3 OS=Schizosaccharomyces pombe (strain 972 / AT... [more]
Match NameE-valueIdentityDescription
XP_022145424.10.099.72urea-proton symporter DUR3 [Momordica charantia][more]
XP_038876583.10.088.94urea-proton symporter DUR3 [Benincasa hispida] >XP_038876584.1 urea-proton sympo... [more]
XP_008448582.10.087.96PREDICTED: urea-proton symporter DUR3 [Cucumis melo] >KAA0052983.1 urea-proton s... [more]
XP_011650307.10.088.10urea-proton symporter DUR3 [Cucumis sativus] >KGN55684.1 hypothetical protein Cs... [more]
XP_023005805.10.086.36LOW QUALITY PROTEIN: urea-proton symporter DUR3 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1CV760.099.72urea-proton symporter DUR3 OS=Momordica charantia OX=3673 GN=LOC111014871 PE=3 S... [more]
A0A5A7UAL40.087.96Urea-proton symporter DUR3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A1S3BKN10.087.96urea-proton symporter DUR3 OS=Cucumis melo OX=3656 GN=LOC103490716 PE=3 SV=1[more]
A0A0A0L3R60.088.10Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G004500 PE=3 SV=1[more]
A0A6J1KYE30.086.36LOW QUALITY PROTEIN: urea-proton symporter DUR3 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
AT5G45380.16.2e-31075.82solute:sodium symporters;urea transmembrane transporters [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001734Sodium/solute symporterTIGRFAMTIGR00813TIGR00813coord: 78..502
e-value: 4.3E-58
score: 195.3
IPR001734Sodium/solute symporterPFAMPF00474SSFcoord: 79..502
e-value: 4.8E-20
score: 71.8
IPR001734Sodium/solute symporterPROSITEPS50283NA_SOLUT_SYMP_3coord: 43..516
score: 33.103397
IPR038377Sodium/glucose symporter superfamilyGENE3D1.20.1730.10Sodium/glucose cotransportercoord: 67..608
e-value: 3.7E-79
score: 268.3
IPR031155Urea active transporterPANTHERPTHR46154FAMILY NOT NAMEDcoord: 24..689
NoneNo IPR availablePANTHERPTHR46154:SF4UREA ACTIVE TRANSPORTERcoord: 24..689
NoneNo IPR availableCDDcd11476SLC5sbd_DUR3coord: 44..554
e-value: 0.0
score: 586.09

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g1384.1MC10g1384.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071918 urea transmembrane transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015204 urea transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity