MC10g1375 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g1375
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionATP-dependent Clp protease proteolytic subunit
LocationMC10: 17227526 .. 17233091 (-)
RNA-Seq ExpressionMC10g1375
SyntenyMC10g1375
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GACATTTTGTAATTACCATAAACACATGGGTGGATTATTGGTATGAAAGTTAAGAACTTGGGGCATTTTTAATATTATTACAAACACATGGGTGGATTATTGAAATTACCAAAGTTTCTACACGTTTTCCTGTATAGCTCACCCAACCAAATCCCCCAAAGCCATCGTCTCTCCGCTTCTTCCTGGTTTCAGAGCTGAAGCAGAGCAGTGCTGTAAATCCATGGAGCTTCTTTCACGATGTTCTACTCTCATTCCTCACGCATCTTCCCTCAGCCTTCCTAATTCTCATCCAAAGCCATCCAATTTCTATCCCAAGCTCAAATCCAGTCTCTCTTTTCCTTCCGCTTGCTCAGTCCTCAAGGCAACCGCTCTTAAACCCTCGAGAACTCTAGCTTCGCCGTGCTCCGTCCTCATGACGGCTCCACAGACGCCGGATGTCGCCCGGAGAGGCGCCGAGACTGACGCCATGGGACTGCTTCTCAGGGAGAGGATCGTCTTCTTAGGGAATAGCATCGATGACTTTGTGGCCGATGCTATTATCAGCCAGCTCTTGCTATTGGATGCTCAGGACTCTACCAAAGATATCAGGCTCTTCATTAATTCCCCCGGCGGCTCTCTCAGGTATGCACGGGCTGATTTTTGGTGCATAATTTTGTTTGTGAAGATATGGAATATGAGATTCAGGTTTTTCTTTATTTTTTTCCAAATCAGTTGGATAATTGAACTTCTATGAATATGGATGTTGTGCCCAAGTTTCATATAGAATGAGTTAATATGCTTTCCTTAGCGCTGAATGCAAGAGACGGATAGTTGAACTCTGCTTCTCGCAATCCTATATAAAGATCTCACCATCTTCACCTGTACCGCAAACAGTTAGCCTACGGGTGGGTGTATGTAGTCCGGTGACGAGTCAGGTCACTGTAAATTTAAGTCTGTTACAGTTATGTAAACATATTTAAAATTGGAAGGAACTGATTGCATGAGAGTTAGTCTACCTTGTTAGATGGAACTGGGCTTGTGCTCTTATTTCCTTGGGTGGCATAGGAGTTGGTAAATGTGAGGACTAGAGACACCGCCATCCTCTCAAGGTGGATGTGCATATTAGTGCATAGAAAGAACGCCCTTTGAAGAAGCATAATCAAGAGTAAATATGGGATTGGCAACCTTTGGAGAAGCTCAATTCATTGGTCCAAGATTCGACATTGCTAAAACAATGGATTTCTTTGACCACTGTGTCTTCTTAAAGTTTGGTGATGGTCTGAGGATTAAATTTTGGACAGACGCTTGGCTTGATCCTTCTTGTCCAAGCCATATTCTCTAAATATTTTCTCAATTTCAAATTCTGGTGACTTTTTTTTTGTTGTTTTTTTATTGGGGAAATTTTTGGAAGAGACAGTCCAAATTTGGATTTTCAGCTCAGGAGAAATCTTTTTGATAAAGAGCCGGAGGAATGGTTAATCTTTATTCTGAAAGATGGAAAGCAATCGGCTAAATCATGGTGAAGATAATTTTGTTGAAAGTTTGAATGCTTTGGGCTGTTTCCTTGCAAATCTGCCAACCTTCAGCTTAATAGTAGAGAAGGCAGGTCGGAGAAACCATTTCTTCTTTGATTTGGGAGGAAACAATCCTTAAAAGACTTTGTTCTTCCTTTGAAATATGGCTTACAAGAGTCTAAACACTTGTGATTGGATTCCTTCCATGGCTTTATATCCTTCTGTTTGACCTGTCTGCTTATCTAATGAAGAAACCATGGATCATTTGTTTATCAATTGTAAAAGGGATGGAGCTTACTCTCAGACGTTTGGTTTTGTTGGTGCTATTCTAAAGATATAAACACTTAGTAGCATGAAATTCTCTCTTGTTGGTGTTTTTGCAAAAAGCCAGAATTTTGTTGGCAAATGCTTCTGGAGCTTTGCTGTGGTATGTATATTTAGAAGGAAAGTAACAACAGAATTTTTTAAGATAGGTATATGCTTTGATGTTTTTTTGAGACTGTATAGTTTAGTGCAACTACGTGGAGTTCTCTCCAAGAATTTTGTGATTACAAGGCTTCTTTGATAAACCTAGATTGGGGGTACTTTGGCTTAGAAAGTTGTAGGACAAGATGGGTATCCCAGCCTTACAAAAGGACAATGGCAGATTTGGAAGAAACCTCTCTAGTTTATCTTGATTTTAAAGTAAAGAAGAACAGAAACCTATAAGACACTTTAGCTCACAAAGATTATATAAAAGCTACAAAAATGATTCTAGAGGTATTTCTTTCCATTCAATATCTACAAATTGGAATATCATTGAGGCAGATTTAATGATTTTAGATGTGTAATTGTGGGGAAAGAGCTACTCTTTGGCTTCTTGAAGGGAGAGAGAAACCAAATAATTTTTGAAGATGAGGCAACTCCAGTGGATTCTTTTTGTAATAGTGTACGGCAAACCTCTAGATGGTATTCAAACCACAAAAAATTCTTTTGTAACTATGGTATTCACTTGATTAGCAACGATGGGAAGGCTTTGCTTTTTATGCAGATCCTTGGGCAGAGGTTTTCTCTACCCCGGCCCCTAGGTTGGTTGCTTCTTTCTTGTGCTGAATTCAATTCTTGGTTCCTATAAAAAAATGATTTTAGATGTTTCCTTAGCACTATAGCTTCTTATTATATCTTCCAAGAAGTCTTTCATTCTCCTAAAAGTCAATTAAACTCAAAGATATCCAATGATTAATCACCCTGCTCAAGAAAAAAGAACAATATATTCTTCAAAATTTTCCCATGAGTCACCTTTTTGAGTCAAGATACTATCATGGATGTCTGGCACACAATCCATTGGTATCGGTCTTCCTAAGAGAGGTTAACCTAATGGTTGAAACCTTGTGAATCATGTCAACAAAATTTATAATGGGAATTTATAAGCAATATGTTCCACAACGGATTTACATGCGAAGAATCCCAATCTCTATAATGACCAATTTCCTAAATAAATACTCATAGGGCAAGGACTAGACTAACACTCCAAAAAGAGCAAGCACATTCGCCAAATCAGAATATTTTAAAAAAATAAATAAACATCCATATTTCTTGTTCTTTCCCTATTACATTCTCAGTGGCAAATGCATAGAGGTGGGGGAAGTGTGAAGATACCCAAGCTTCCGTAATTACTAAAGTATGGCTGTGATGGGTCTATTTGCCTGGTATCTTTTGAATGTGTTTCTTGGCGTCACGAAAAAAAATTGATACGTAGGTGAGTCATAAGTGTCTAAACACACGGATAATTCCCTATAAATTCAGAATGTATGTTTTCAAATAATCTCCATACTAATAGAGAACTTTATTCTATTTGTATCAAGTTTACTTTGCTCTGGATCAAACTTAGTGCTACAGCAGTACAAAATATCTTGAATCATGTGATCTACATGTAATGATGCAACCAATCACTTACAACCATTGGAATCAGAACTCAATATCTCTATTCAAATTTCAAAAAAGAACTGTTGTCTCTTTCATATTCTTCTCTCCTTTGGAGAGGGGATGTGCCTTTTGGGCCACCACCGGAGATATTTGAAGACTTTTTACTGTCTGGAAATGAATGTACTCATCTACTCTATGTCTCTACATGAATGGAAAGATTAGCAAACTGTGGGAACACATACATATCTTCCTTCCCTGATTATTTATTTAATCTCCGTTTTATTCTTGTTTCCATGGTTTGCTGGCCTCTTTGTACGTTAGGATATCTTCTTGAGTAAATTAATATTTATGTGGCTAATGGATGATCTTTTCCTGATTTTTCTCCCCATCTTTGATGCACAACTACTTTTAGGTAAATTCGTTCCCTTAGGACCATACCTGAGAGTTTCCCACCTTTTACAACTCTAATCGTAAGCTGTTAAATGTATATTGCTATTTAACTACATAAGGATGAATGTGAGAAGTGTAAGCAGAAGATGTACTCTGCCTAGGTCGGTTCTTTTGTCAGAAAAGGTTATGTTTTTAAGTGCCACATCAATTTATCCTCGTTTTGATTTAGGAACACATCTTTCAACTGTGCAGTAAACTGAGCCCAGTCTCCCAATTCTCTACCAGGCAGATTCTTTATCAGCCCAATGTCCCTAGCTTGGGTGTTTTCTTTCGAGCAATCACTTACTACCACTCCTTTTGTATTAGCAACGGTGTAAATATTGTTGAAGATCATAAACCAGTTATCTTCCTAGAATCTAATTCTAAGCCTGTTTCCCAGCTTAGAAAGCAAATCTTTTTTCTAACAAAACCAACACATTTAGCCTAGATCAGGCCAGGGGCAGAGGGGAGACTAGAGTACATGGTTCTCTTTGTTTTCTCTGACTTCAAGGACTAAAAATTTAAAAACCGGGAGTGTCACTATTATGAGTATATGGATGTTTCGGTTCTCGAGGTGCTTCAGGGGTGGTTTTTGAAAGGACAAGCTAAAATCCTTTGGGGTATGAGTTCTGGTACTAGTAATATTTTTTTGAGCATTTGGAAGGGAAAAAATCAACGAGTCTTCAACAACACGGCTCGTCCTTTTGATGTCTTTTGTGAAAGTGTACAAACTAATACGTCTTCTTACAGGAGGGGCTTCCACGTCCCTGTTGTTCCTTTCCTTTTCTCCATTTTTCAATACATATTCTTTCTTATGAAAAGGAAAAAAGAATTTTATTGTCATTATTTATTTGAAGCTGGAAATATTGATTGAATATGCTTTTTTAAACATGTATTTTTTGCTCATTCAGCCTTCAATGAGCGACCACCTGATGTCATGTATTTTTGCATCAAATTCGTTAGCATTTTTGCATAATATTTGTTGTTCGGACTGATTTCCTTTTATTAATGTCATTTCCGTTAGTGCTACAATGGCAATCTACGATGTCGTACAGCTCGTGAGGGCTGATGTCTCCACAATTGGACTAGGCATTGCAGCCTCAACAGCTTCCATAATCCTCGGCGGTGGCACTAAAGGCAAGCGTCTCGCAATGCCTAATGCACGTATTATGGTTCATCAACCTCTTGGAGGGGCGAGTGGTCAAGCAATAGATGTGGAAATTCAAGCACGTGAAATCATGCATAACAAGAACAACGTAACGAGAATTATCTCCGAATTCACTGGCCATCCGTTTGATAAAGTCCAAAAAGATATTGATAGGGATCGTTATATGTCGCCCATAGAGGCGGTTGAATATGGATTGATTGATGGAGTTATTGACAAAGATAGCATTATACCTCTCATGCCAGTGCCAGAAAGAGTGAAGGCAAGTTTAAATTATGAAGAAATTAGTAAAGATCCCAGAAAATTTTTGACACCAGATGTCCCCGACGACGAGATATACTAGTTTTCGGCGTTTCGGTAGACCTTTCTTTAGGCCCTTGATTTATATGATTTACATGTAGAATCTTATGTATGCAATTCATGGTGAGTGGAAATCAGAAATACCTTTAGGTATCAAAACTTCTCTACTCAAGTAAAAGGTATTGTGAATTTGTACCTTTTTTGTTTGACATTTTCAAATGAGCATTCTGAGAAAAGAAAAATTGGCATTGAATCATATTTTGTATTGTCAGTG

mRNA sequence

GACATTTTGTAATTACCATAAACACATGGGTGGATTATTGGTATGAAAGTTAAGAACTTGGGGCATTTTTAATATTATTACAAACACATGGGTGGATTATTGAAATTACCAAAGTTTCTACACGTTTTCCTGTATAGCTCACCCAACCAAATCCCCCAAAGCCATCGTCTCTCCGCTTCTTCCTGGTTTCAGAGCTGAAGCAGAGCAGTGCTGTAAATCCATGGAGCTTCTTTCACGATGTTCTACTCTCATTCCTCACGCATCTTCCCTCAGCCTTCCTAATTCTCATCCAAAGCCATCCAATTTCTATCCCAAGCTCAAATCCAGTCTCTCTTTTCCTTCCGCTTGCTCAGTCCTCAAGGCAACCGCTCTTAAACCCTCGAGAACTCTAGCTTCGCCGTGCTCCGTCCTCATGACGGCTCCACAGACGCCGGATGTCGCCCGGAGAGGCGCCGAGACTGACGCCATGGGACTGCTTCTCAGGGAGAGGATCGTCTTCTTAGGGAATAGCATCGATGACTTTGTGGCCGATGCTATTATCAGCCAGCTCTTGCTATTGGATGCTCAGGACTCTACCAAAGATATCAGGCTCTTCATTAATTCCCCCGGCGGCTCTCTCAGTGCTACAATGGCAATCTACGATGTCGTACAGCTCGTGAGGGCTGATGTCTCCACAATTGGACTAGGCATTGCAGCCTCAACAGCTTCCATAATCCTCGGCGGTGGCACTAAAGGCAAGCGTCTCGCAATGCCTAATGCACGTATTATGGTTCATCAACCTCTTGGAGGGGCGAGTGGTCAAGCAATAGATGTGGAAATTCAAGCACGTGAAATCATGCATAACAAGAACAACGTAACGAGAATTATCTCCGAATTCACTGGCCATCCGTTTGATAAAGTCCAAAAAGATATTGATAGGGATCGTTATATGTCGCCCATAGAGGCGGTTGAATATGGATTGATTGATGGAGTTATTGACAAAGATAGCATTATACCTCTCATGCCAGTGCCAGAAAGAGTGAAGGCAAGTTTAAATTATGAAGAAATTAGTAAAGATCCCAGAAAATTTTTGACACCAGATGTCCCCGACGACGAGATATACTAGTTTTCGGCGTTTCGGTAGACCTTTCTTTAGGCCCTTGATTTATATGATTTACATGTAGAATCTTATGTATGCAATTCATGGTGAGTGGAAATCAGAAATACCTTTAGGTATCAAAACTTCTCTACTCAAGTAAAAGGTATTGTGAATTTGTACCTTTTTTGTTTGACATTTTCAAATGAGCATTCTGAGAAAAGAAAAATTGGCATTGAATCATATTTTGTATTGTCAGTG

Coding sequence (CDS)

ATGGAGCTTCTTTCACGATGTTCTACTCTCATTCCTCACGCATCTTCCCTCAGCCTTCCTAATTCTCATCCAAAGCCATCCAATTTCTATCCCAAGCTCAAATCCAGTCTCTCTTTTCCTTCCGCTTGCTCAGTCCTCAAGGCAACCGCTCTTAAACCCTCGAGAACTCTAGCTTCGCCGTGCTCCGTCCTCATGACGGCTCCACAGACGCCGGATGTCGCCCGGAGAGGCGCCGAGACTGACGCCATGGGACTGCTTCTCAGGGAGAGGATCGTCTTCTTAGGGAATAGCATCGATGACTTTGTGGCCGATGCTATTATCAGCCAGCTCTTGCTATTGGATGCTCAGGACTCTACCAAAGATATCAGGCTCTTCATTAATTCCCCCGGCGGCTCTCTCAGTGCTACAATGGCAATCTACGATGTCGTACAGCTCGTGAGGGCTGATGTCTCCACAATTGGACTAGGCATTGCAGCCTCAACAGCTTCCATAATCCTCGGCGGTGGCACTAAAGGCAAGCGTCTCGCAATGCCTAATGCACGTATTATGGTTCATCAACCTCTTGGAGGGGCGAGTGGTCAAGCAATAGATGTGGAAATTCAAGCACGTGAAATCATGCATAACAAGAACAACGTAACGAGAATTATCTCCGAATTCACTGGCCATCCGTTTGATAAAGTCCAAAAAGATATTGATAGGGATCGTTATATGTCGCCCATAGAGGCGGTTGAATATGGATTGATTGATGGAGTTATTGACAAAGATAGCATTATACCTCTCATGCCAGTGCCAGAAAGAGTGAAGGCAAGTTTAAATTATGAAGAAATTAGTAAAGATCCCAGAAAATTTTTGACACCAGATGTCCCCGACGACGAGATATACTAG

Protein sequence

MELLSRCSTLIPHASSLSLPNSHPKPSNFYPKLKSSLSFPSACSVLKATALKPSRTLASPCSVLMTAPQTPDVARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTKDIRLFINSPGGSLSATMAIYDVVQLVRADVSTIGLGIAASTASIILGGGTKGKRLAMPNARIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFDKVQKDIDRDRYMSPIEAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY
Homology
BLAST of MC10g1375 vs. ExPASy Swiss-Prot
Match: Q94B60 (ATP-dependent Clp protease proteolytic subunit 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CLPP4 PE=1 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 2.0e-102
Identity = 211/301 (70.10%), Postives = 239/301 (79.40%), Query Frame = 0

Query: 1   MELLSRCSTLIP-------HASSLSLPNSHPKPSNFYPKLKSSLSFPSACSVLKATALKP 60
           M  LS  S+L P       ++SS +  +S PKP+N Y K    +S P     L+ T+  P
Sbjct: 1   MGTLSLSSSLKPSLVSSRLNSSSSASSSSFPKPNNLYLKPTKLISPP-----LRTTSPSP 60

Query: 61  SRTLASPCSVLMTAPQTPDVARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLL 120
            R   +  S+ M+  QT + A RGAE+D MGLLLRERIVFLG+SIDDFVADAI+SQLLLL
Sbjct: 61  LR--FANASIEMS--QTQESAIRGAESDVMGLLLRERIVFLGSSIDDFVADAIMSQLLLL 120

Query: 121 DAQDSTKDIRLFINSPGGSLSATMAIYDVVQLVRADVSTIGLGIAASTASIILGGGTKGK 180
           DA+D  KDI+LFINSPGGSLSATMAIYDVVQLVRADVSTI LGIAASTASIILG GTKGK
Sbjct: 121 DAKDPKKDIKLFINSPGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGAGTKGK 180

Query: 181 RLAMPNARIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFDKVQKDIDR 240
           R AMPN RIM+HQPLGGASGQAIDVEIQA+E+MHNKNNVT II+  T   F++V KDIDR
Sbjct: 181 RFAMPNTRIMIHQPLGGASGQAIDVEIQAKEVMHNKNNVTSIIAGCTSRSFEQVLKDIDR 240

Query: 241 DRYMSPIEAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEI 295
           DRYMSPIEAVEYGLIDGVID DSIIPL PVP+RVK  +NYEEISKDP KFLTP++PDDEI
Sbjct: 241 DRYMSPIEAVEYGLIDGVIDGDSIIPLEPVPDRVKPRVNYEEISKDPMKFLTPEIPDDEI 292

BLAST of MC10g1375 vs. ExPASy Swiss-Prot
Match: Q3ANI8 (ATP-dependent Clp protease proteolytic subunit 1 OS=Synechococcus sp. (strain CC9605) OX=110662 GN=clpP1 PE=3 SV=2)

HSP 1 Score: 216.9 bits (551), Expect = 3.2e-55
Identity = 108/195 (55.38%), Postives = 144/195 (73.85%), Query Frame = 0

Query: 60  PCSVLMTAPQTPDVARRGAET-DAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDS 119
           P S     P   + + RG    D    LLRERI+FLG  +DD VADA+++Q+L L+A+D 
Sbjct: 2   PVSAPGPLPTVVEQSGRGDRAFDIYSRLLRERIIFLGTGVDDAVADALVAQMLFLEAEDP 61

Query: 120 TKDIRLFINSPGGSLSATMAIYDVVQLVRADVSTIGLGIAASTASIILGGGTKGKRLAMP 179
            KDI+++INSPGGS++A +AIYD +Q V  DV TI  G+AAS  + +L GGTKGKRLA+P
Sbjct: 62  EKDIQIYINSPGGSVTAGLAIYDTMQQVAPDVVTICYGLAASMGAFLLSGGTKGKRLALP 121

Query: 180 NARIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFDKVQKDIDRDRYMS 239
           NARIM+HQPLGGA GQA+D+EIQA+EI++ K  +  +++E TG P DK+ +D DRD ++S
Sbjct: 122 NARIMIHQPLGGAQGQAVDIEIQAKEILYLKETLNGLMAEHTGQPLDKISEDTDRDYFLS 181

Query: 240 PIEAVEYGLIDGVID 254
           P EAVEYGLID V+D
Sbjct: 182 PAEAVEYGLIDRVVD 196

BLAST of MC10g1375 vs. ExPASy Swiss-Prot
Match: Q3B0U1 (ATP-dependent Clp protease proteolytic subunit 2 OS=Synechococcus sp. (strain CC9902) OX=316279 GN=clpP2 PE=3 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 7.0e-55
Identity = 107/195 (54.87%), Postives = 143/195 (73.33%), Query Frame = 0

Query: 60  PCSVLMTAPQTPDVARRGAET-DAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDS 119
           P S     P   + + RG    D    LLRERI+FLG  +DD VADA+++Q+L L+A+D 
Sbjct: 2   PISAPGPLPTVVEQSGRGDRAFDIYSRLLRERIIFLGTGVDDAVADALVAQMLFLEAEDP 61

Query: 120 TKDIRLFINSPGGSLSATMAIYDVVQLVRADVSTIGLGIAASTASIILGGGTKGKRLAMP 179
            KDI++++NSPGGS++A +AIYD +Q V  DV TI  G+AAS  + +L GGTKGKRLA+P
Sbjct: 62  EKDIQIYVNSPGGSVTAGLAIYDTMQQVAPDVVTICYGLAASMGAFLLSGGTKGKRLALP 121

Query: 180 NARIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFDKVQKDIDRDRYMS 239
           NARIM+HQPLGGA GQA+D+EIQA+EI+  K  +  +++E TG P DK+ +D DRD ++S
Sbjct: 122 NARIMIHQPLGGAQGQAVDIEIQAKEILFLKETLNGLLAEHTGQPLDKISEDTDRDYFLS 181

Query: 240 PIEAVEYGLIDGVID 254
           P EAVEYGLID V+D
Sbjct: 182 PAEAVEYGLIDRVVD 196

BLAST of MC10g1375 vs. ExPASy Swiss-Prot
Match: Q7V992 (ATP-dependent Clp protease proteolytic subunit 1 OS=Prochlorococcus marinus (strain MIT 9313) OX=74547 GN=clpP1 PE=3 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 7.8e-54
Identity = 104/187 (55.61%), Postives = 141/187 (75.40%), Query Frame = 0

Query: 68  PQTPDVARRGAET-DAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTKDIRLFI 127
           P   + + RG    D    LLRERI+FLG  +DD VADA+++Q+L L+A+D  KDI+++I
Sbjct: 27  PTVVEQSGRGERAFDIYSRLLRERIIFLGTGVDDQVADALVAQMLFLEAEDPEKDIQIYI 86

Query: 128 NSPGGSLSATMAIYDVVQLVRADVSTIGLGIAASTASIILGGGTKGKRLAMPNARIMVHQ 187
           NSPGGS++A +AIYD +Q V  DV TI  G+AAS  + +L GGTKGKRLA+PNARIM+HQ
Sbjct: 87  NSPGGSVTAGLAIYDTMQQVAPDVVTICYGLAASMGAFLLCGGTKGKRLALPNARIMIHQ 146

Query: 188 PLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFDKVQKDIDRDRYMSPIEAVEYG 247
           PLGGA GQA+D+EIQA+EI+  K  +  +++E TG P +K+ +D DRD ++SP +AVEYG
Sbjct: 147 PLGGAQGQAVDIEIQAKEILFLKETLNGLLAEHTGQPLNKIAEDTDRDHFLSPAKAVEYG 206

Query: 248 LIDGVID 254
           LID V+D
Sbjct: 207 LIDRVVD 213

BLAST of MC10g1375 vs. ExPASy Swiss-Prot
Match: Q7UA36 (ATP-dependent Clp protease proteolytic subunit 1 OS=Synechococcus sp. (strain WH8102) OX=84588 GN=clpP1 PE=3 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 2.3e-53
Identity = 105/195 (53.85%), Postives = 142/195 (72.82%), Query Frame = 0

Query: 60  PCSVLMTAPQTPDVARRGAET-DAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDS 119
           P S     P   + + RG    D    LLRERI+FLG  +DD VADA+++Q+L L+A+D 
Sbjct: 19  PISAPGPLPTVVEQSGRGDRAFDIYSRLLRERIIFLGTGVDDAVADALVAQMLFLEAEDP 78

Query: 120 TKDIRLFINSPGGSLSATMAIYDVVQLVRADVSTIGLGIAASTASIILGGGTKGKRLAMP 179
            KDI+++INSPGGS++A +AIYD +Q V  DV TI  G+AAS  + +L GG KGKRLA+P
Sbjct: 79  EKDIQIYINSPGGSVTAGLAIYDTMQQVAPDVVTICYGLAASMGAFLLSGGCKGKRLALP 138

Query: 180 NARIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFDKVQKDIDRDRYMS 239
           NARIM+HQPLGGA GQA+D+EIQA+EI+  K  +  ++++ TG P DK+ +D DRD ++S
Sbjct: 139 NARIMIHQPLGGAQGQAVDIEIQAKEILFLKETLNGLMADHTGQPLDKIAEDTDRDYFLS 198

Query: 240 PIEAVEYGLIDGVID 254
           P EAV+YGLID V+D
Sbjct: 199 PAEAVQYGLIDRVVD 213

BLAST of MC10g1375 vs. NCBI nr
Match: XP_022145352.1 (ATP-dependent Clp protease proteolytic subunit 4, chloroplastic [Momordica charantia])

HSP 1 Score: 563 bits (1451), Expect = 5.86e-202
Identity = 294/294 (100.00%), Postives = 294/294 (100.00%), Query Frame = 0

Query: 1   MELLSRCSTLIPHASSLSLPNSHPKPSNFYPKLKSSLSFPSACSVLKATALKPSRTLASP 60
           MELLSRCSTLIPHASSLSLPNSHPKPSNFYPKLKSSLSFPSACSVLKATALKPSRTLASP
Sbjct: 1   MELLSRCSTLIPHASSLSLPNSHPKPSNFYPKLKSSLSFPSACSVLKATALKPSRTLASP 60

Query: 61  CSVLMTAPQTPDVARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120
           CSVLMTAPQTPDVARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK
Sbjct: 61  CSVLMTAPQTPDVARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120

Query: 121 DIRLFINSPGGSLSATMAIYDVVQLVRADVSTIGLGIAASTASIILGGGTKGKRLAMPNA 180
           DIRLFINSPGGSLSATMAIYDVVQLVRADVSTIGLGIAASTASIILGGGTKGKRLAMPNA
Sbjct: 121 DIRLFINSPGGSLSATMAIYDVVQLVRADVSTIGLGIAASTASIILGGGTKGKRLAMPNA 180

Query: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFDKVQKDIDRDRYMSPI 240
           RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFDKVQKDIDRDRYMSPI
Sbjct: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFDKVQKDIDRDRYMSPI 240

Query: 241 EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY 294
           EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY
Sbjct: 241 EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY 294

BLAST of MC10g1375 vs. NCBI nr
Match: XP_038876611.1 (ATP-dependent Clp protease proteolytic subunit 4, chloroplastic [Benincasa hispida])

HSP 1 Score: 525 bits (1351), Expect = 1.02e-186
Identity = 276/294 (93.88%), Postives = 280/294 (95.24%), Query Frame = 0

Query: 1   MELLSRCSTLIPHASSLSLPNSHPKPSNFYPKLKSSLSFPSACSVLKATALKPSRTLASP 60
           MELLSRCSTL+PHASS+ LP SH KPSNF PKLKS+LSFPSA SVLK TA KPSRT A P
Sbjct: 1   MELLSRCSTLLPHASSIGLPTSHGKPSNFLPKLKSTLSFPSASSVLKTTAPKPSRTPAPP 60

Query: 61  CSVLMTAPQTPDVARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120
           CSVLMTAPQTPD ARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK
Sbjct: 61  CSVLMTAPQTPDAARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120

Query: 121 DIRLFINSPGGSLSATMAIYDVVQLVRADVSTIGLGIAASTASIILGGGTKGKRLAMPNA 180
           DIRLFINS GGSLSATMAIYDVVQLVRADVSTI LGIAASTASIILGGGTKGKRLAMPNA
Sbjct: 121 DIRLFINSAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA 180

Query: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFDKVQKDIDRDRYMSPI 240
           RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPF+KVQKDIDRDRYMSPI
Sbjct: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMSPI 240

Query: 241 EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY 294
           EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDP KFL PDVPDDEIY
Sbjct: 241 EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPTKFLNPDVPDDEIY 294

BLAST of MC10g1375 vs. NCBI nr
Match: XP_008448589.1 (PREDICTED: ATP-dependent Clp protease proteolytic subunit 4, chloroplastic [Cucumis melo])

HSP 1 Score: 522 bits (1345), Expect = 8.07e-186
Identity = 275/294 (93.54%), Postives = 283/294 (96.26%), Query Frame = 0

Query: 1   MELLSRCSTLIPHASSLSLPNSHPKPSNFYPKLKSSLSFPSACSVLKATALKPSRTLASP 60
           MELLSRCSTLIP ASSL LPNSH KPSNF+PKLKS+LSFPSA SVLK TALKPSRTL  P
Sbjct: 1   MELLSRCSTLIPQASSLGLPNSHGKPSNFFPKLKSTLSFPSASSVLKTTALKPSRTLPPP 60

Query: 61  CSVLMTAPQTPDVARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120
           CSV MTAPQTPD +RRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK
Sbjct: 61  CSV-MTAPQTPDASRRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120

Query: 121 DIRLFINSPGGSLSATMAIYDVVQLVRADVSTIGLGIAASTASIILGGGTKGKRLAMPNA 180
           DIRLFINS GGSLSATMAIYDVVQLVRADVSTI LGIAASTASIILGGGTKGKRLAMPNA
Sbjct: 121 DIRLFINSAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA 180

Query: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFDKVQKDIDRDRYMSPI 240
           RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPF+KVQKDIDRDRYMSPI
Sbjct: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMSPI 240

Query: 241 EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY 294
           EAVEYGLIDGVID+DSIIPL+PVPERVKA+LNYEE+SKDPRKFLTPDVPDDEIY
Sbjct: 241 EAVEYGLIDGVIDRDSIIPLVPVPERVKATLNYEEMSKDPRKFLTPDVPDDEIY 293

BLAST of MC10g1375 vs. NCBI nr
Match: KAG7015794.1 (ATP-dependent Clp protease proteolytic subunit 4, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 520 bits (1338), Expect = 9.76e-185
Identity = 274/294 (93.20%), Postives = 280/294 (95.24%), Query Frame = 0

Query: 1   MELLSRCSTLIPHASSLSLPNSHPKPSNFYPKLKSSLSFPSACSVLKATALKPSRTLASP 60
           MELLSRCSTLIPHASSL L NSH KPSNFYPKLKSSLSFPSA SVLK TA KPSRTLA  
Sbjct: 1   MELLSRCSTLIPHASSLGLLNSHGKPSNFYPKLKSSLSFPSASSVLKTTARKPSRTLAPS 60

Query: 61  CSVLMTAPQTPDVARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120
           CSV MT PQTPD A+RGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK
Sbjct: 61  CSVHMTGPQTPDAAQRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120

Query: 121 DIRLFINSPGGSLSATMAIYDVVQLVRADVSTIGLGIAASTASIILGGGTKGKRLAMPNA 180
           DIRLFINS GGSLSATMAIYDVVQLVRADVSTI LGIAASTASIILGGGTKGKRLAMPNA
Sbjct: 121 DIRLFINSSGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA 180

Query: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFDKVQKDIDRDRYMSPI 240
           RIMVHQPLGGASGQAIDVEIQAREIMHNKNN+TRIISEFTGHPF+KVQKDIDRDRYMSPI
Sbjct: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNITRIISEFTGHPFEKVQKDIDRDRYMSPI 240

Query: 241 EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY 294
           EAVEYGLIDGVIDKDSIIPL+PVPERVKASLNY EISKDP+KFL+PDVPDDEIY
Sbjct: 241 EAVEYGLIDGVIDKDSIIPLVPVPERVKASLNYIEISKDPKKFLSPDVPDDEIY 294

BLAST of MC10g1375 vs. NCBI nr
Match: XP_022923515.1 (ATP-dependent Clp protease proteolytic subunit 4, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 520 bits (1340), Expect = 2.69e-184
Identity = 274/294 (93.20%), Postives = 281/294 (95.58%), Query Frame = 0

Query: 1   MELLSRCSTLIPHASSLSLPNSHPKPSNFYPKLKSSLSFPSACSVLKATALKPSRTLASP 60
           MELLSRCSTLIPHASSL L NSH KPSNFYPKLKSSLSFPSA SVLK TA KPSRTLA+ 
Sbjct: 48  MELLSRCSTLIPHASSLGLLNSHGKPSNFYPKLKSSLSFPSASSVLKTTARKPSRTLATS 107

Query: 61  CSVLMTAPQTPDVARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120
           CSV MT PQTPD A+RGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK
Sbjct: 108 CSVHMTGPQTPDAAQRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 167

Query: 121 DIRLFINSPGGSLSATMAIYDVVQLVRADVSTIGLGIAASTASIILGGGTKGKRLAMPNA 180
           DIRLFINS GGSLSATMAIYDVVQLVRADVSTI LGIAASTASIILGGGTKGKRLAMPNA
Sbjct: 168 DIRLFINSSGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA 227

Query: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFDKVQKDIDRDRYMSPI 240
           RIMVHQPLGGASGQAIDVEIQAREIMHNKNN+TRIISEFTGHPF+KVQKDIDRDRYMSPI
Sbjct: 228 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNITRIISEFTGHPFEKVQKDIDRDRYMSPI 287

Query: 241 EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY 294
           EAVEYGLIDGVIDKDSIIPL+PVPERVKASLNY EISKDP+KFL+PDVPDDEIY
Sbjct: 288 EAVEYGLIDGVIDKDSIIPLVPVPERVKASLNYIEISKDPKKFLSPDVPDDEIY 341

BLAST of MC10g1375 vs. ExPASy TrEMBL
Match: A0A6J1CWB9 (ATP-dependent Clp protease proteolytic subunit OS=Momordica charantia OX=3673 GN=LOC111014827 PE=3 SV=1)

HSP 1 Score: 563 bits (1451), Expect = 2.84e-202
Identity = 294/294 (100.00%), Postives = 294/294 (100.00%), Query Frame = 0

Query: 1   MELLSRCSTLIPHASSLSLPNSHPKPSNFYPKLKSSLSFPSACSVLKATALKPSRTLASP 60
           MELLSRCSTLIPHASSLSLPNSHPKPSNFYPKLKSSLSFPSACSVLKATALKPSRTLASP
Sbjct: 1   MELLSRCSTLIPHASSLSLPNSHPKPSNFYPKLKSSLSFPSACSVLKATALKPSRTLASP 60

Query: 61  CSVLMTAPQTPDVARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120
           CSVLMTAPQTPDVARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK
Sbjct: 61  CSVLMTAPQTPDVARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120

Query: 121 DIRLFINSPGGSLSATMAIYDVVQLVRADVSTIGLGIAASTASIILGGGTKGKRLAMPNA 180
           DIRLFINSPGGSLSATMAIYDVVQLVRADVSTIGLGIAASTASIILGGGTKGKRLAMPNA
Sbjct: 121 DIRLFINSPGGSLSATMAIYDVVQLVRADVSTIGLGIAASTASIILGGGTKGKRLAMPNA 180

Query: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFDKVQKDIDRDRYMSPI 240
           RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFDKVQKDIDRDRYMSPI
Sbjct: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFDKVQKDIDRDRYMSPI 240

Query: 241 EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY 294
           EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY
Sbjct: 241 EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY 294

BLAST of MC10g1375 vs. ExPASy TrEMBL
Match: A0A1S3BJF4 (ATP-dependent Clp protease proteolytic subunit OS=Cucumis melo OX=3656 GN=LOC103490719 PE=3 SV=1)

HSP 1 Score: 522 bits (1345), Expect = 3.91e-186
Identity = 275/294 (93.54%), Postives = 283/294 (96.26%), Query Frame = 0

Query: 1   MELLSRCSTLIPHASSLSLPNSHPKPSNFYPKLKSSLSFPSACSVLKATALKPSRTLASP 60
           MELLSRCSTLIP ASSL LPNSH KPSNF+PKLKS+LSFPSA SVLK TALKPSRTL  P
Sbjct: 1   MELLSRCSTLIPQASSLGLPNSHGKPSNFFPKLKSTLSFPSASSVLKTTALKPSRTLPPP 60

Query: 61  CSVLMTAPQTPDVARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120
           CSV MTAPQTPD +RRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK
Sbjct: 61  CSV-MTAPQTPDASRRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120

Query: 121 DIRLFINSPGGSLSATMAIYDVVQLVRADVSTIGLGIAASTASIILGGGTKGKRLAMPNA 180
           DIRLFINS GGSLSATMAIYDVVQLVRADVSTI LGIAASTASIILGGGTKGKRLAMPNA
Sbjct: 121 DIRLFINSAGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA 180

Query: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFDKVQKDIDRDRYMSPI 240
           RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPF+KVQKDIDRDRYMSPI
Sbjct: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFEKVQKDIDRDRYMSPI 240

Query: 241 EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY 294
           EAVEYGLIDGVID+DSIIPL+PVPERVKA+LNYEE+SKDPRKFLTPDVPDDEIY
Sbjct: 241 EAVEYGLIDGVIDRDSIIPLVPVPERVKATLNYEEMSKDPRKFLTPDVPDDEIY 293

BLAST of MC10g1375 vs. ExPASy TrEMBL
Match: A0A6J1EC18 (ATP-dependent Clp protease proteolytic subunit OS=Cucurbita moschata OX=3662 GN=LOC111431188 PE=3 SV=1)

HSP 1 Score: 520 bits (1340), Expect = 1.30e-184
Identity = 274/294 (93.20%), Postives = 281/294 (95.58%), Query Frame = 0

Query: 1   MELLSRCSTLIPHASSLSLPNSHPKPSNFYPKLKSSLSFPSACSVLKATALKPSRTLASP 60
           MELLSRCSTLIPHASSL L NSH KPSNFYPKLKSSLSFPSA SVLK TA KPSRTLA+ 
Sbjct: 48  MELLSRCSTLIPHASSLGLLNSHGKPSNFYPKLKSSLSFPSASSVLKTTARKPSRTLATS 107

Query: 61  CSVLMTAPQTPDVARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120
           CSV MT PQTPD A+RGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK
Sbjct: 108 CSVHMTGPQTPDAAQRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 167

Query: 121 DIRLFINSPGGSLSATMAIYDVVQLVRADVSTIGLGIAASTASIILGGGTKGKRLAMPNA 180
           DIRLFINS GGSLSATMAIYDVVQLVRADVSTI LGIAASTASIILGGGTKGKRLAMPNA
Sbjct: 168 DIRLFINSSGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA 227

Query: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFDKVQKDIDRDRYMSPI 240
           RIMVHQPLGGASGQAIDVEIQAREIMHNKNN+TRIISEFTGHPF+KVQKDIDRDRYMSPI
Sbjct: 228 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNITRIISEFTGHPFEKVQKDIDRDRYMSPI 287

Query: 241 EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY 294
           EAVEYGLIDGVIDKDSIIPL+PVPERVKASLNY EISKDP+KFL+PDVPDDEIY
Sbjct: 288 EAVEYGLIDGVIDKDSIIPLVPVPERVKASLNYIEISKDPKKFLSPDVPDDEIY 341

BLAST of MC10g1375 vs. ExPASy TrEMBL
Match: A0A6J1HNH6 (ATP-dependent Clp protease proteolytic subunit OS=Cucurbita maxima OX=3661 GN=LOC111465249 PE=3 SV=1)

HSP 1 Score: 513 bits (1321), Expect = 1.84e-182
Identity = 270/294 (91.84%), Postives = 278/294 (94.56%), Query Frame = 0

Query: 1   MELLSRCSTLIPHASSLSLPNSHPKPSNFYPKLKSSLSFPSACSVLKATALKPSRTLASP 60
           MELLSRCSTLIPHASSL L NSH KPSNFYPKLKS LSFPSA SVLK TA KPSRTLA+ 
Sbjct: 1   MELLSRCSTLIPHASSLGLLNSHGKPSNFYPKLKSGLSFPSASSVLKTTARKPSRTLATS 60

Query: 61  CSVLMTAPQTPDVARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120
            SV MT PQTPD A+RGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK
Sbjct: 61  SSVHMTGPQTPDAAQRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120

Query: 121 DIRLFINSPGGSLSATMAIYDVVQLVRADVSTIGLGIAASTASIILGGGTKGKRLAMPNA 180
           DIRLFINS GGSLSATMAIYDV+QLVRADVSTI LGIAASTASIILGGGTKGKRLAMPNA
Sbjct: 121 DIRLFINSSGGSLSATMAIYDVIQLVRADVSTIALGIAASTASIILGGGTKGKRLAMPNA 180

Query: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFDKVQKDIDRDRYMSPI 240
           RIMVHQPLGGASGQAIDVEIQAREIMHNKNN+TRIISEFTGHPF+KVQKDIDRDRYMSPI
Sbjct: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNITRIISEFTGHPFEKVQKDIDRDRYMSPI 240

Query: 241 EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY 294
           EAVEYGLIDGVIDKDSIIPL+PVPERVKASLNY EISKDP+KF +PDVPDDEIY
Sbjct: 241 EAVEYGLIDGVIDKDSIIPLVPVPERVKASLNYIEISKDPKKFFSPDVPDDEIY 294

BLAST of MC10g1375 vs. ExPASy TrEMBL
Match: A0A6J1FAG6 (ATP-dependent Clp protease proteolytic subunit OS=Cucurbita moschata OX=3662 GN=LOC111443555 PE=3 SV=1)

HSP 1 Score: 508 bits (1309), Expect = 1.24e-180
Identity = 266/294 (90.48%), Postives = 277/294 (94.22%), Query Frame = 0

Query: 1   MELLSRCSTLIPHASSLSLPNSHPKPSNFYPKLKSSLSFPSACSVLKATALKPSRTLASP 60
           MELLSRCSTL+PHAS+LSLPNSH K S FYPK KSSLSFPS+ SVLKATALKPSRTLA P
Sbjct: 1   MELLSRCSTLVPHASTLSLPNSHGKSSVFYPKRKSSLSFPSSSSVLKATALKPSRTLAPP 60

Query: 61  CSVLMTAPQTPDVARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTK 120
           CSVLMT PQTPD AR GAETDAMGLLLRERIVFLGN+IDDFVADAIISQLLLLDAQDSTK
Sbjct: 61  CSVLMTTPQTPDSARTGAETDAMGLLLRERIVFLGNNIDDFVADAIISQLLLLDAQDSTK 120

Query: 121 DIRLFINSPGGSLSATMAIYDVVQLVRADVSTIGLGIAASTASIILGGGTKGKRLAMPNA 180
           DIRLFINS GGSLSATMAI DV+QLVRADVSTI LGIAASTASIILGGGTKGKR AMPNA
Sbjct: 121 DIRLFINSSGGSLSATMAIIDVLQLVRADVSTIALGIAASTASIILGGGTKGKRFAMPNA 180

Query: 181 RIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFDKVQKDIDRDRYMSPI 240
           RIMVHQPLGG SGQAIDVEIQAREIMHNKNN+ RIIS +TGHPF+KVQKDIDRDRYMSPI
Sbjct: 181 RIMVHQPLGGTSGQAIDVEIQAREIMHNKNNIIRIISNYTGHPFEKVQKDIDRDRYMSPI 240

Query: 241 EAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEIY 294
           EAVEYGLIDGVIDKD+IIPLM +PERVKA+LNYEEISKDPRKFLTPDVPDDEIY
Sbjct: 241 EAVEYGLIDGVIDKDTIIPLMQLPERVKATLNYEEISKDPRKFLTPDVPDDEIY 294

BLAST of MC10g1375 vs. TAIR 10
Match: AT5G45390.1 (CLP protease P4 )

HSP 1 Score: 373.6 bits (958), Expect = 1.4e-103
Identity = 211/301 (70.10%), Postives = 239/301 (79.40%), Query Frame = 0

Query: 1   MELLSRCSTLIP-------HASSLSLPNSHPKPSNFYPKLKSSLSFPSACSVLKATALKP 60
           M  LS  S+L P       ++SS +  +S PKP+N Y K    +S P     L+ T+  P
Sbjct: 1   MGTLSLSSSLKPSLVSSRLNSSSSASSSSFPKPNNLYLKPTKLISPP-----LRTTSPSP 60

Query: 61  SRTLASPCSVLMTAPQTPDVARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLL 120
            R   +  S+ M+  QT + A RGAE+D MGLLLRERIVFLG+SIDDFVADAI+SQLLLL
Sbjct: 61  LR--FANASIEMS--QTQESAIRGAESDVMGLLLRERIVFLGSSIDDFVADAIMSQLLLL 120

Query: 121 DAQDSTKDIRLFINSPGGSLSATMAIYDVVQLVRADVSTIGLGIAASTASIILGGGTKGK 180
           DA+D  KDI+LFINSPGGSLSATMAIYDVVQLVRADVSTI LGIAASTASIILG GTKGK
Sbjct: 121 DAKDPKKDIKLFINSPGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGAGTKGK 180

Query: 181 RLAMPNARIMVHQPLGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFDKVQKDIDR 240
           R AMPN RIM+HQPLGGASGQAIDVEIQA+E+MHNKNNVT II+  T   F++V KDIDR
Sbjct: 181 RFAMPNTRIMIHQPLGGASGQAIDVEIQAKEVMHNKNNVTSIIAGCTSRSFEQVLKDIDR 240

Query: 241 DRYMSPIEAVEYGLIDGVIDKDSIIPLMPVPERVKASLNYEEISKDPRKFLTPDVPDDEI 295
           DRYMSPIEAVEYGLIDGVID DSIIPL PVP+RVK  +NYEEISKDP KFLTP++PDDEI
Sbjct: 241 DRYMSPIEAVEYGLIDGVIDGDSIIPLEPVPDRVKPRVNYEEISKDPMKFLTPEIPDDEI 292

BLAST of MC10g1375 vs. TAIR 10
Match: AT1G66670.1 (CLP protease proteolytic subunit 3 )

HSP 1 Score: 200.7 bits (509), Expect = 1.7e-51
Identity = 108/227 (47.58%), Postives = 151/227 (66.52%), Query Frame = 0

Query: 31  PKLKSSLSFPSACSVLKATALKPSRTLASPCSV----LMTAPQTPDVARRGAETDAMGLL 90
           PK        S+ S+ K     P +TL+S   V    + +  Q+P       E D   +L
Sbjct: 35  PKTSKPFCVRSSMSLSK----PPRQTLSSNWDVSSFSIDSVAQSPSRLPSFEELDTTNML 94

Query: 91  LRERIVFLGNSIDDFVADAIISQLLLLDAQDSTKDIRLFINSPGGSLSATMAIYDVVQLV 150
           LR+RIVFLG+ +DD  AD +ISQLLLLDA+DS +DI LFINSPGGS++A M IYD ++  
Sbjct: 95  LRQRIVFLGSQVDDMTADLVISQLLLLDAEDSERDITLFINSPGGSITAGMGIYDAMKQC 154

Query: 151 RADVSTIGLGIAASTASIILGGGTKGKRLAMPNARIMVHQPLGGASGQAIDVEIQAREIM 210
           +ADVST+ LG+AAS  + +L  G+KGKR  MPN+++M+HQPLG A G+A ++ I+ RE+M
Sbjct: 155 KADVSTVCLGLAASMGAFLLASGSKGKRYCMPNSKVMIHQPLGTAGGKATEMSIRIREMM 214

Query: 211 HNKNNVTRIISEFTGHPFDKVQKDIDRDRYMSPIEAVEYGLIDGVID 254
           ++K  + +I S  TG P  +++ D DRD +++P EA EYGLID VID
Sbjct: 215 YHKIKLNKIFSRITGKPESEIESDTDRDNFLNPWEAKEYGLIDAVID 257

BLAST of MC10g1375 vs. TAIR 10
Match: AT1G02560.1 (nuclear encoded CLP protease 5 )

HSP 1 Score: 181.4 bits (459), Expect = 1.0e-45
Identity = 87/167 (52.10%), Postives = 125/167 (74.85%), Query Frame = 0

Query: 86  LLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTKDIRLFINSPGGSLSATMAIYDVVQL 145
           L + RI+  G ++DD +A+ I++QLL LDA D TKDI +++NSPGGS++A MAI+D ++ 
Sbjct: 119 LFQYRIIRCGGAVDDDMANIIVAQLLYLDAVDPTKDIVMYVNSPGGSVTAGMAIFDTMRH 178

Query: 146 VRADVSTIGLGIAASTASIILGGGTKGKRLAMPNARIMVHQPLGGASGQAIDVEIQAREI 205
           +R DVST+ +G+AAS  + +L  GTKGKR ++PN+RIM+HQPLGGA G   D++IQA E+
Sbjct: 179 IRPDVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQTDIDIQANEM 238

Query: 206 MHNKNNVTRIISEFTGHPFDKVQKDIDRDRYMSPIEAVEYGLIDGVI 253
           +H+K N+   ++  TG   +K+ +D DRD +MS  EA EYGLIDGVI
Sbjct: 239 LHHKANLNGYLAYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVI 285

BLAST of MC10g1375 vs. TAIR 10
Match: AT1G11750.1 (CLP protease proteolytic subunit 6 )

HSP 1 Score: 162.5 bits (410), Expect = 5.0e-40
Identity = 78/188 (41.49%), Postives = 116/188 (61.70%), Query Frame = 0

Query: 68  PQTPDVARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTKDIRLFIN 127
           P  P V   G   D   +L R RI+F+G  I+  VA  +ISQL+ L + D   DI +++N
Sbjct: 83  PVMPSVMTPGGPLDLSSVLFRNRIIFIGQPINAQVAQRVISQLVTLASIDDKSDILMYLN 142

Query: 128 SPGGSLSATMAIYDVVQLVRADVSTIGLGIAASTASIILGGGTKGKRLAMPNARIMVHQP 187
            PGGS  + +AIYD +  ++  V T+  G+AAS  +++L GG KG R AMPN R+M+HQP
Sbjct: 143 CPGGSTYSVLAIYDCMSWIKPKVGTVAFGVAASQGALLLAGGEKGMRYAMPNTRVMIHQP 202

Query: 188 LGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFDKVQKDIDRDRYMSPIEAVEYGL 247
             G  G   DV  Q  E +  +  + R+ + FTG P +KVQ+  +RDR++S  EA+E+GL
Sbjct: 203 QTGCGGHVEDVRRQVNEAIEARQKIDRMYAAFTGQPLEKVQQYTERDRFLSASEALEFGL 262

Query: 248 IDGVIDKD 256
           IDG+++ +
Sbjct: 263 IDGLLETE 270

BLAST of MC10g1375 vs. TAIR 10
Match: AT1G11750.2 (CLP protease proteolytic subunit 6 )

HSP 1 Score: 162.5 bits (410), Expect = 5.0e-40
Identity = 78/188 (41.49%), Postives = 116/188 (61.70%), Query Frame = 0

Query: 68  PQTPDVARRGAETDAMGLLLRERIVFLGNSIDDFVADAIISQLLLLDAQDSTKDIRLFIN 127
           P  P V   G   D   +L R RI+F+G  I+  VA  +ISQL+ L + D   DI +++N
Sbjct: 101 PVMPSVMTPGGPLDLSSVLFRNRIIFIGQPINAQVAQRVISQLVTLASIDDKSDILMYLN 160

Query: 128 SPGGSLSATMAIYDVVQLVRADVSTIGLGIAASTASIILGGGTKGKRLAMPNARIMVHQP 187
            PGGS  + +AIYD +  ++  V T+  G+AAS  +++L GG KG R AMPN R+M+HQP
Sbjct: 161 CPGGSTYSVLAIYDCMSWIKPKVGTVAFGVAASQGALLLAGGEKGMRYAMPNTRVMIHQP 220

Query: 188 LGGASGQAIDVEIQAREIMHNKNNVTRIISEFTGHPFDKVQKDIDRDRYMSPIEAVEYGL 247
             G  G   DV  Q  E +  +  + R+ + FTG P +KVQ+  +RDR++S  EA+E+GL
Sbjct: 221 QTGCGGHVEDVRRQVNEAIEARQKIDRMYAAFTGQPLEKVQQYTERDRFLSASEALEFGL 280

Query: 248 IDGVIDKD 256
           IDG+++ +
Sbjct: 281 IDGLLETE 288

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94B602.0e-10270.10ATP-dependent Clp protease proteolytic subunit 4, chloroplastic OS=Arabidopsis t... [more]
Q3ANI83.2e-5555.38ATP-dependent Clp protease proteolytic subunit 1 OS=Synechococcus sp. (strain CC... [more]
Q3B0U17.0e-5554.87ATP-dependent Clp protease proteolytic subunit 2 OS=Synechococcus sp. (strain CC... [more]
Q7V9927.8e-5455.61ATP-dependent Clp protease proteolytic subunit 1 OS=Prochlorococcus marinus (str... [more]
Q7UA362.3e-5353.85ATP-dependent Clp protease proteolytic subunit 1 OS=Synechococcus sp. (strain WH... [more]
Match NameE-valueIdentityDescription
XP_022145352.15.86e-202100.00ATP-dependent Clp protease proteolytic subunit 4, chloroplastic [Momordica chara... [more]
XP_038876611.11.02e-18693.88ATP-dependent Clp protease proteolytic subunit 4, chloroplastic [Benincasa hispi... [more]
XP_008448589.18.07e-18693.54PREDICTED: ATP-dependent Clp protease proteolytic subunit 4, chloroplastic [Cucu... [more]
KAG7015794.19.76e-18593.20ATP-dependent Clp protease proteolytic subunit 4, chloroplastic [Cucurbita argyr... [more]
XP_022923515.12.69e-18493.20ATP-dependent Clp protease proteolytic subunit 4, chloroplastic-like [Cucurbita ... [more]
Match NameE-valueIdentityDescription
A0A6J1CWB92.84e-202100.00ATP-dependent Clp protease proteolytic subunit OS=Momordica charantia OX=3673 GN... [more]
A0A1S3BJF43.91e-18693.54ATP-dependent Clp protease proteolytic subunit OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6J1EC181.30e-18493.20ATP-dependent Clp protease proteolytic subunit OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1HNH61.84e-18291.84ATP-dependent Clp protease proteolytic subunit OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1FAG61.24e-18090.48ATP-dependent Clp protease proteolytic subunit OS=Cucurbita moschata OX=3662 GN=... [more]
Match NameE-valueIdentityDescription
AT5G45390.11.4e-10370.10CLP protease P4 [more]
AT1G66670.11.7e-5147.58CLP protease proteolytic subunit 3 [more]
AT1G02560.11.0e-4552.10nuclear encoded CLP protease 5 [more]
AT1G11750.15.0e-4041.49CLP protease proteolytic subunit 6 [more]
AT1G11750.25.0e-4041.49CLP protease proteolytic subunit 6 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001907ATP-dependent Clp protease proteolytic subunitPRINTSPR00127CLPPROTEASEPcoord: 152..169
score: 50.14
coord: 173..192
score: 57.75
coord: 121..141
score: 58.93
coord: 81..96
score: 46.88
coord: 230..249
score: 56.38
IPR001907ATP-dependent Clp protease proteolytic subunitHAMAPMF_00444ClpPcoord: 66..255
score: 37.187035
IPR001907ATP-dependent Clp protease proteolytic subunitCDDcd07017S14_ClpP_2coord: 81..251
e-value: 2.88184E-98
score: 284.334
IPR023562Clp protease proteolytic subunit /Translocation-enhancing protein TepAPFAMPF00574CLP_proteasecoord: 80..254
e-value: 4.3E-72
score: 241.8
IPR023562Clp protease proteolytic subunit /Translocation-enhancing protein TepAPANTHERPTHR10381ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNITcoord: 60..291
NoneNo IPR availableGENE3D3.90.226.10coord: 59..257
e-value: 1.0E-69
score: 236.2
NoneNo IPR availablePANTHERPTHR10381:SF24ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT 4, CHLOROPLASTICcoord: 60..291
IPR033135ClpP, histidine active sitePROSITEPS00382CLP_PROTEASE_HIScoord: 174..187
IPR029045ClpP/crotonase-like domain superfamilySUPERFAMILY52096ClpP/crotonasecoord: 80..257

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g1375.1MC10g1375.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006515 protein quality control for misfolded or incompletely synthesized proteins
biological_process GO:0006508 proteolysis
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0009368 endopeptidase Clp complex
cellular_component GO:0009526 plastid envelope
molecular_function GO:0051117 ATPase binding
molecular_function GO:0004176 ATP-dependent peptidase activity
molecular_function GO:0004252 serine-type endopeptidase activity