MC10g1331 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g1331
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionInositol-tetrakisphosphate 1-kinase
LocationMC10: 16823491 .. 16824528 (+)
RNA-Seq ExpressionMC10g1331
SyntenyMC10g1331
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGATCGTAGATTCTGCGTAGGGTATGCTCTGGCTCCGAAGAAGCAGCAGAGTTTCATCCAGGACTCGTTAGTGAGCCTGGCGGCGTCGCGAGGGGTCGATCTTGTTCGGATTGACACTGACCGGCCACTTCTTGATCAAGGCCCATTCGATTGCATTCTCCATAAGTTTTACGGCGAGGATTGGAGGAAGCAGCTGGTGGAGTTCAGAGCTAAGAACCCTAATGCGTTCATTTTGGACTCGCCGGACGCGATTGAGAGACTTCACAACCGGATTTCGATGCTTCAGGTGGTTTCAGAGTTGAAGATTGATAATCCGGATGAATCGTTTGGGATCCCGAAGCAGATTGTGATTTATGATAAGGAGACGTTGTTTGATCGGCAGGCGTGGGAGGGTTTGAAATTCCCTGTCATTGCTAAGCCATTGGTGGCCGATGGCAGTGCCAAATCTCACAAAATGGCGCTCGTCTTCAACCACGATTGTTTGAACAAGCTTAAGCCTCCGATAGTTCTGCAGGAGTTTGTGAACCATGGGGGCGTTATCTTTAAGGTTTACGTTGCTGGGCAATATGTGAAATGTGTGAAGAGGAAGTCTCTGCCCGACGAGCCGGAGGAGAAACTGGGGAATGTAGAGGGATTGTTGTCGTTTTCGCAGGTCTCGAATTTGACTACTCGTGAGAAAACTGATGATAAGTACTACAAGATGATGCAGCTTGATGATACAGAGATGCCACCGTTGAGTTTCGTCACCGACATCGCTAGGGGGTTGCGGCGCTCCATGAACTTGAATCTTTTTAACTTTGACGTGATTCGGGACTCAAAAATTGGGACTCGGTACCTTATAATTGACATTAACTATTTCCCTGGGTATGCAAAAATGCCGGGTTATGAGAAGGTTTTGACCGATTTTTTCTGTGATTTAGCGCAGAAGAAAGAAGCAGTGAAGAATTCTGAACAAGAAACGAGAAAGATCGCCAGTGATGAGGATGATGGAGGTCTATCAGATGACAGGGAAAACAAAGAAACCCCTGTTCAA

mRNA sequence

ATGGCGGATCGTAGATTCTGCGTAGGGTATGCTCTGGCTCCGAAGAAGCAGCAGAGTTTCATCCAGGACTCGTTAGTGAGCCTGGCGGCGTCGCGAGGGGTCGATCTTGTTCGGATTGACACTGACCGGCCACTTCTTGATCAAGGCCCATTCGATTGCATTCTCCATAAGTTTTACGGCGAGGATTGGAGGAAGCAGCTGGTGGAGTTCAGAGCTAAGAACCCTAATGCGTTCATTTTGGACTCGCCGGACGCGATTGAGAGACTTCACAACCGGATTTCGATGCTTCAGGTGGTTTCAGAGTTGAAGATTGATAATCCGGATGAATCGTTTGGGATCCCGAAGCAGATTGTGATTTATGATAAGGAGACGTTGTTTGATCGGCAGGCGTGGGAGGGTTTGAAATTCCCTGTCATTGCTAAGCCATTGGTGGCCGATGGCAGTGCCAAATCTCACAAAATGGCGCTCGTCTTCAACCACGATTGTTTGAACAAGCTTAAGCCTCCGATAGTTCTGCAGGAGTTTGTGAACCATGGGGGCGTTATCTTTAAGGTTTACGTTGCTGGGCAATATGTGAAATGTGTGAAGAGGAAGTCTCTGCCCGACGAGCCGGAGGAGAAACTGGGGAATGTAGAGGGATTGTTGTCGTTTTCGCAGGTCTCGAATTTGACTACTCGTGAGAAAACTGATGATAAGTACTACAAGATGATGCAGCTTGATGATACAGAGATGCCACCGTTGAGTTTCGTCACCGACATCGCTAGGGGGTTGCGGCGCTCCATGAACTTGAATCTTTTTAACTTTGACGTGATTCGGGACTCAAAAATTGGGACTCGGTACCTTATAATTGACATTAACTATTTCCCTGGGTATGCAAAAATGCCGGGTTATGAGAAGGTTTTGACCGATTTTTTCTGTGATTTAGCGCAGAAGAAAGAAGCAGTGAAGAATTCTGAACAAGAAACGAGAAAGATCGCCAGTGATGAGGATGATGGAGGTCTATCAGATGACAGGGAAAACAAAGAAACCCCTGTTCAA

Coding sequence (CDS)

ATGGCGGATCGTAGATTCTGCGTAGGGTATGCTCTGGCTCCGAAGAAGCAGCAGAGTTTCATCCAGGACTCGTTAGTGAGCCTGGCGGCGTCGCGAGGGGTCGATCTTGTTCGGATTGACACTGACCGGCCACTTCTTGATCAAGGCCCATTCGATTGCATTCTCCATAAGTTTTACGGCGAGGATTGGAGGAAGCAGCTGGTGGAGTTCAGAGCTAAGAACCCTAATGCGTTCATTTTGGACTCGCCGGACGCGATTGAGAGACTTCACAACCGGATTTCGATGCTTCAGGTGGTTTCAGAGTTGAAGATTGATAATCCGGATGAATCGTTTGGGATCCCGAAGCAGATTGTGATTTATGATAAGGAGACGTTGTTTGATCGGCAGGCGTGGGAGGGTTTGAAATTCCCTGTCATTGCTAAGCCATTGGTGGCCGATGGCAGTGCCAAATCTCACAAAATGGCGCTCGTCTTCAACCACGATTGTTTGAACAAGCTTAAGCCTCCGATAGTTCTGCAGGAGTTTGTGAACCATGGGGGCGTTATCTTTAAGGTTTACGTTGCTGGGCAATATGTGAAATGTGTGAAGAGGAAGTCTCTGCCCGACGAGCCGGAGGAGAAACTGGGGAATGTAGAGGGATTGTTGTCGTTTTCGCAGGTCTCGAATTTGACTACTCGTGAGAAAACTGATGATAAGTACTACAAGATGATGCAGCTTGATGATACAGAGATGCCACCGTTGAGTTTCGTCACCGACATCGCTAGGGGGTTGCGGCGCTCCATGAACTTGAATCTTTTTAACTTTGACGTGATTCGGGACTCAAAAATTGGGACTCGGTACCTTATAATTGACATTAACTATTTCCCTGGGTATGCAAAAATGCCGGGTTATGAGAAGGTTTTGACCGATTTTTTCTGTGATTTAGCGCAGAAGAAAGAAGCAGTGAAGAATTCTGAACAAGAAACGAGAAAGATCGCCAGTGATGAGGATGATGGAGGTCTATCAGATGACAGGGAAAACAAAGAAACCCCTGTTCAA

Protein sequence

MADRRFCVGYALAPKKQQSFIQDSLVSLAASRGVDLVRIDTDRPLLDQGPFDCILHKFYGEDWRKQLVEFRAKNPNAFILDSPDAIERLHNRISMLQVVSELKIDNPDESFGIPKQIVIYDKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGGVIFKVYVAGQYVKCVKRKSLPDEPEEKLGNVEGLLSFSQVSNLTTREKTDDKYYKMMQLDDTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKVLTDFFCDLAQKKEAVKNSEQETRKIASDEDDGGLSDDRENKETPVQ
Homology
BLAST of MC10g1331 vs. ExPASy Swiss-Prot
Match: Q84Y01 (Inositol-tetrakisphosphate 1-kinase 1 OS=Zea mays OX=4577 GN=ITPK1 PE=2 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 2.2e-108
Identity = 194/318 (61.01%), Postives = 243/318 (76.42%), Query Frame = 0

Query: 5   RFCVGYALAPKKQQSFIQDSLVSLAASRGVDLVRIDTDRPLLDQGPFDCILHKFYGEDWR 64
           R+ +GYALAPKKQQSFIQ SLV+ AASRG+DLV +D  +PL +QGPF  ++HK YG+DWR
Sbjct: 18  RYVIGYALAPKKQQSFIQPSLVAQAASRGMDLVPVDASQPLAEQGPFHLLIHKLYGDDWR 77

Query: 65  KQLVEFRAKNPNAFILDSPDAIERLHNRISMLQVVSEL-KIDNPDESFGIPKQIVIYDKE 124
            QLV F A++P   I+D P AI+RLHNRISMLQVVSEL    + D +FGIP Q+V+YD  
Sbjct: 78  AQLVAFAARHPAVPIVDPPHAIDRLHNRISMLQVVSELDHAADQDSTFGIPSQVVVYDAA 137

Query: 125 TLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGGVIF 184
            L D      L+FP+IAKPLVADG+AKSHKM+LV++ + L KL+PP+VLQEFVNHGGVIF
Sbjct: 138 ALADFGLLAALRFPLIAKPLVADGTAKSHKMSLVYHREGLGKLRPPLVLQEFVNHGGVIF 197

Query: 185 KVYVAGQYVKCVKRKSLPDEPEEKLGNVEGLLSFSQVSNLTTREKTDDKYYKMMQLDDTE 244
           KVYV G +V CVKR+SLPD   E   + +G +SFSQVSNL T E+T ++YY    L+D  
Sbjct: 198 KVYVVGGHVTCVKRRSLPDVSPEDDASAQGSVSFSQVSNLPT-ERTAEEYYGEKSLEDAV 257

Query: 245 MPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKVLTD 304
           +PP +F+  IA GLRR++ L LFNFD+IRD + G RYL+IDINYFPGYAKMPGYE VLTD
Sbjct: 258 VPPAAFINQIAGGLRRALGLQLFNFDMIRDVRAGDRYLVIDINYFPGYAKMPGYETVLTD 317

Query: 305 FFCDLAQKKEAVKNSEQE 322
           FF ++   K+ V N ++E
Sbjct: 318 FFWEMVH-KDGVGNQQEE 333

BLAST of MC10g1331 vs. ExPASy Swiss-Prot
Match: A0JJZ6 (Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. indica OX=39946 GN=ITPK5 PE=3 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 1.1e-107
Identity = 194/319 (60.82%), Postives = 240/319 (75.24%), Query Frame = 0

Query: 4   RRFCVGYALAPKKQQSFIQDSLVSLAASRGVDLVRIDTDRPLLDQGPFDCILHKFYGEDW 63
           RR+ +GYALAPKKQQSFIQ SLVS AA RG+DLV +D  RPL +QGPF  ++HK YGE+W
Sbjct: 14  RRYLIGYALAPKKQQSFIQPSLVSRAAGRGMDLVPVDPSRPLPEQGPFHLLIHKLYGEEW 73

Query: 64  RKQLVEFRAKNPNAFILDSPDAIERLHNRISMLQVVSELKID---NPDESFGIPKQIVIY 123
           R QL  F A +P   ++D P AI+RLHNRISMLQVVSEL +    +   +FGIP Q+V+Y
Sbjct: 74  RGQLDAFSAAHPAVPVVDPPHAIDRLHNRISMLQVVSELDVPLHAHHHHTFGIPSQVVVY 133

Query: 124 DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG 183
           D   L D      L+FP+IAKPLVADG+AKSHKM+LV++ + L KL+PP+VLQEFVNHGG
Sbjct: 134 DAAALSDSGLLAALRFPLIAKPLVADGTAKSHKMSLVYHREGLRKLRPPLVLQEFVNHGG 193

Query: 184 VIFKVYVAGQYVKCVKRKSLPDEPEEKL--GNVEGLLSFSQVSNLTTREKTDDKYYKMMQ 243
           VIFKVYV G +V CVKR+SLPD   + L   + EG LSFSQVSNL   E+T  +YY  M+
Sbjct: 194 VIFKVYVVGAHVTCVKRRSLPDVSSDVLQDASAEGSLSFSQVSNL-PNERTAQEYYDDMR 253

Query: 244 LDDTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYE 303
           L+D  MPP +F+ DIA  LRR++ L+LFNFD+IRD++ G RYL+IDINYFPGYAKMPGYE
Sbjct: 254 LEDAIMPPTAFINDIAAALRRALGLHLFNFDMIRDARAGDRYLVIDINYFPGYAKMPGYE 313

Query: 304 KVLTDFFCDLAQKKEAVKN 318
            VLTDFF ++  K +   N
Sbjct: 314 TVLTDFFWEMVHKDDDTPN 331

BLAST of MC10g1331 vs. ExPASy Swiss-Prot
Match: Q7XBW0 (Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=ITPK5 PE=2 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 1.1e-107
Identity = 194/319 (60.82%), Postives = 240/319 (75.24%), Query Frame = 0

Query: 4   RRFCVGYALAPKKQQSFIQDSLVSLAASRGVDLVRIDTDRPLLDQGPFDCILHKFYGEDW 63
           RR+ +GYALAPKKQQSFIQ SLVS AA RG+DLV +D  RPL +QGPF  ++HK YGE+W
Sbjct: 14  RRYLIGYALAPKKQQSFIQPSLVSRAAGRGMDLVPVDPSRPLPEQGPFHLLIHKLYGEEW 73

Query: 64  RKQLVEFRAKNPNAFILDSPDAIERLHNRISMLQVVSELKID---NPDESFGIPKQIVIY 123
           R QL  F A +P   ++D P AI+RLHNRISMLQVVSEL +    +   +FGIP Q+V+Y
Sbjct: 74  RGQLDAFSAAHPAVPVVDPPHAIDRLHNRISMLQVVSELDVPLHAHHHHTFGIPSQVVVY 133

Query: 124 DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG 183
           D   L D      L+FP+IAKPLVADG+AKSHKM+LV++ + L KL+PP+VLQEFVNHGG
Sbjct: 134 DAAALSDSGLLAALRFPLIAKPLVADGTAKSHKMSLVYHREGLRKLRPPLVLQEFVNHGG 193

Query: 184 VIFKVYVAGQYVKCVKRKSLPDEPEEKL--GNVEGLLSFSQVSNLTTREKTDDKYYKMMQ 243
           VIFKVYV G +V CVKR+SLPD   + L   + EG LSFSQVSNL   E+T  +YY  M+
Sbjct: 194 VIFKVYVVGAHVTCVKRRSLPDVSSDVLQDASAEGSLSFSQVSNL-PNERTAQEYYDDMR 253

Query: 244 LDDTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYE 303
           L+D  MPP +F+ DIA  LRR++ L+LFNFD+IRD++ G RYL+IDINYFPGYAKMPGYE
Sbjct: 254 LEDAIMPPTAFINDIAAALRRALGLHLFNFDMIRDARAGDRYLVIDINYFPGYAKMPGYE 313

Query: 304 KVLTDFFCDLAQKKEAVKN 318
            VLTDFF ++  K +   N
Sbjct: 314 TVLTDFFWEMVHKDDDTPN 331

BLAST of MC10g1331 vs. ExPASy Swiss-Prot
Match: Q9SBA5 (Inositol-tetrakisphosphate 1-kinase 1 OS=Arabidopsis thaliana OX=3702 GN=ITPK1 PE=1 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 1.9e-104
Identity = 186/312 (59.62%), Postives = 232/312 (74.36%), Query Frame = 0

Query: 5   RFCVGYALAPKKQQSFIQDSLVSLAASRGVDLVRIDTDRPLLDQGPFDCILHKFYGEDWR 64
           R+ VGYALA KKQ SFIQ SL+  +  RG+DLV++D  + LL+QG  DCI+HK Y   W+
Sbjct: 8   RYLVGYALAAKKQHSFIQPSLIEHSRQRGIDLVKLDPTKSLLEQGKLDCIIHKLYDVYWK 67

Query: 65  KQLVEFRAKNPNAFILDSPDAIERLHNRISMLQVVSELKIDNPD-ESFGIPKQIVIYDKE 124
           + L EFR K P   ++D P+AIERLHNR+SML+V+++L+    D E FG+P+Q+V+ D  
Sbjct: 68  ENLHEFREKCPGVPVIDLPEAIERLHNRVSMLEVITQLRFPVSDSERFGVPEQVVVMDSS 127

Query: 125 TLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGGVIF 184
            L    A   LKFPVIAKPL ADGSAKSHKM L+++ + +  LK PIVLQEFVNHGGVIF
Sbjct: 128 VLSGGGALGELKFPVIAKPLDADGSAKSHKMFLIYDQEGMKILKAPIVLQEFVNHGGVIF 187

Query: 185 KVYVAGQYVKCVKRKSLPDEPEEKLGNVEGLLSFSQVSNLTTREKTDDKYYKMMQLDDTE 244
           KVYV G +VKCVKR+SLPD  EEK+G  +G L FSQ+SNLT +E  + +Y +   L+  E
Sbjct: 188 KVYVVGDHVKCVKRRSLPDISEEKIGTSKGSLPFSQISNLTAQEDKNIEYGEDRSLEKVE 247

Query: 245 MPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKVLTD 304
           MPPLSF+TD+A+ +R SM LNLFNFDVIRD+K   RYLIIDINYFPGYAKMP YE VLT+
Sbjct: 248 MPPLSFLTDLAKAMRESMGLNLFNFDVIRDAKDANRYLIIDINYFPGYAKMPSYEPVLTE 307

Query: 305 FFCDLAQKKEAV 316
           FF D+  KK  V
Sbjct: 308 FFWDMVTKKNHV 319

BLAST of MC10g1331 vs. ExPASy Swiss-Prot
Match: A2X4M8 (Inositol-tetrakisphosphate 1-kinase 4 OS=Oryza sativa subsp. indica OX=39946 GN=ITPK4 PE=3 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 8.5e-76
Identity = 155/311 (49.84%), Postives = 199/311 (63.99%), Query Frame = 0

Query: 5   RFCVGYALAPKKQQSFIQDSLVSLAASRGVDLVRIDTDRPLLDQGPFDCILHKFYGEDWR 64
           R+ VGYAL P+K  S ++ SLV+LAA RGV LV +D  RPL +QGPFD ++HK Y   WR
Sbjct: 13  RYTVGYALLPEKVSSVVRPSLVALAADRGVRLVAVDVSRPLAEQGPFDLLVHKMYDRGWR 72

Query: 65  KQLVEFRAKNPNA-FILDSPDAIERLHNRISMLQVVSELKIDNPDESFGIPKQIVIYDKE 124
            QL E  A++P    ++DSP AI+RL +R +ML VVS L+         +P Q+V+ D  
Sbjct: 73  AQLEELAARHPGVPVVVDSPGAIDRLLDRATMLDVVSGLR-----TPVSVPPQVVVSDAA 132

Query: 125 TLFDR-QAWEGLKFPVIAKPLVADGSAKSHKMALVFNHD-CLNKLKPPIVLQEFVNHGGV 184
              D   A   L+FP+IAKPL  DGSA+SH M LV+  D  L  L+ P+VLQEFVNHGGV
Sbjct: 133 ADADELLARAALRFPLIAKPLAVDGSAESHDMRLVYRRDGVLPLLRAPLVLQEFVNHGGV 192

Query: 185 IFKVYVAGQYVKCVKRKSLPDEPEEKLGNV--EGLLSFSQVSNLTTREKTDDKYYKMMQL 244
           +FKVYV G    CV+R SLPD P  +L ++  E  + F+ +SN       DD        
Sbjct: 193 LFKVYVVGDRATCVRRSSLPDVPARRLLDLDAEPSVPFANISNQPLPPPDDDG-----GA 252

Query: 245 DDTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRD--SKIGTRYLIIDINYFPGYAKMPGY 304
            D + P   FV ++ARGLRR + L+LFNFD+IR+   + G RY IIDINYFPGYAKMPGY
Sbjct: 253 ADDDTPAAGFVDEVARGLRRGLGLHLFNFDMIRERSEEHGDRYFIIDINYFPGYAKMPGY 312

Query: 305 EKVLTDFFCDL 309
           E  LTDFF ++
Sbjct: 313 EAALTDFFLEM 313

BLAST of MC10g1331 vs. NCBI nr
Match: XP_022145419.1 (inositol-tetrakisphosphate 1-kinase 1-like isoform X1 [Momordica charantia] >XP_022145420.1 inositol-tetrakisphosphate 1-kinase 1-like isoform X1 [Momordica charantia])

HSP 1 Score: 695 bits (1794), Expect = 1.93e-252
Identity = 345/346 (99.71%), Postives = 345/346 (99.71%), Query Frame = 0

Query: 1   MADRRFCVGYALAPKKQQSFIQDSLVSLAASRGVDLVRIDTDRPLLDQGPFDCILHKFYG 60
           MADRRFCVGYALAPKKQQSFIQDSLVSLAASRGVDLVRIDTDRPLLDQGPFDCILHKFYG
Sbjct: 1   MADRRFCVGYALAPKKQQSFIQDSLVSLAASRGVDLVRIDTDRPLLDQGPFDCILHKFYG 60

Query: 61  EDWRKQLVEFRAKNPNAFILDSPDAIERLHNRISMLQVVSELKIDNPDESFGIPKQIVIY 120
           EDWRKQLVEFRAKNPNAFILDSPDAIERLHNRISMLQVVSELKIDNPDESFGIPKQIVIY
Sbjct: 61  EDWRKQLVEFRAKNPNAFILDSPDAIERLHNRISMLQVVSELKIDNPDESFGIPKQIVIY 120

Query: 121 DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG 180
           DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG
Sbjct: 121 DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG 180

Query: 181 VIFKVYVAGQYVKCVKRKSLPDEPEEKLGNVEGLLSFSQVSNLTTREKTDDKYYKMMQLD 240
           VIFKVYVAGQYVKCVKRKSLPDEPEEKLGNVEGLLSFSQVSNLTTREKTDDKYYKMMQLD
Sbjct: 181 VIFKVYVAGQYVKCVKRKSLPDEPEEKLGNVEGLLSFSQVSNLTTREKTDDKYYKMMQLD 240

Query: 241 DTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV 300
           DTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV
Sbjct: 241 DTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV 300

Query: 301 LTDFFCDLAQKKEAVKNSEQETRKIASDEDDGGLSDDRENKETPVQ 346
           LTDFFCDLAQKKEAVKNSEQETRKI SDEDDGGLSDDRENKETPVQ
Sbjct: 301 LTDFFCDLAQKKEAVKNSEQETRKIVSDEDDGGLSDDRENKETPVQ 346

BLAST of MC10g1331 vs. NCBI nr
Match: XP_038904771.1 (inositol-tetrakisphosphate 1-kinase 1-like [Benincasa hispida])

HSP 1 Score: 641 bits (1653), Expect = 8.70e-231
Identity = 319/358 (89.11%), Postives = 331/358 (92.46%), Query Frame = 0

Query: 1   MADRRFCVGYALAPKKQQSFIQDSLVSLAASRGVDLVRIDTDRPLLDQGPFDCILHKFYG 60
           M +RRFC+GYALAPKK+ SFIQDSLV+LAASRG+DLVRIDTD+PLLDQGPFDCILHKFYG
Sbjct: 1   MEERRFCIGYALAPKKRHSFIQDSLVTLAASRGIDLVRIDTDQPLLDQGPFDCILHKFYG 60

Query: 61  EDWRKQLVEFRAKNPNAFILDSPDAIERLHNRISMLQVVSELKIDNPDESFGIPKQIVIY 120
           EDWRKQLVEFR KNPNAFILDSPD+IERLHNRISMLQVVSELKIDNPDESFGIPKQIVIY
Sbjct: 61  EDWRKQLVEFRVKNPNAFILDSPDSIERLHNRISMLQVVSELKIDNPDESFGIPKQIVIY 120

Query: 121 DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG 180
           DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG
Sbjct: 121 DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG 180

Query: 181 VIFKVYVAGQYVKCVKRKSLPDEPEEKLGNVEGLLSFSQVSNLTTREKTDDKYYKMMQLD 240
           VIFKVYV GQYVKCVKRKSLPDEPE KLGNV+GLLSFSQVSN+T REK DDKYYKMMQLD
Sbjct: 181 VIFKVYVVGQYVKCVKRKSLPDEPEAKLGNVDGLLSFSQVSNMTPREKIDDKYYKMMQLD 240

Query: 241 DTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV 300
           DTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV
Sbjct: 241 DTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV 300

Query: 301 LTDFFCDLAQKKEAVKNSE------------QETRKIASDEDDGGLSDDRENKETPVQ 346
           LTDFFCDLAQKKEA+ N +            QETRKI SDEDDG  S DRE +ET VQ
Sbjct: 301 LTDFFCDLAQKKEALNNPDKKDDVQDKIGCDQETRKIRSDEDDGDQSVDREKEETAVQ 358

BLAST of MC10g1331 vs. NCBI nr
Match: XP_008448667.1 (PREDICTED: inositol-tetrakisphosphate 1-kinase 1-like [Cucumis melo] >KAA0053030.1 inositol-tetrakisphosphate 1-kinase 1-like [Cucumis melo var. makuwa] >TYK11485.1 inositol-tetrakisphosphate 1-kinase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 628 bits (1620), Expect = 1.08e-225
Identity = 315/363 (86.78%), Postives = 330/363 (90.91%), Query Frame = 0

Query: 1   MADRRFCVGYALAPKKQQSFIQDSLVSLAASRGVDLVRIDTDRPLLDQGPFDCILHKFYG 60
           M +RRFC+GYALAPKK+ SFIQDSLV+LAASRG+DLVRIDTDRPLLDQGPFDCILHKFYG
Sbjct: 1   MEERRFCIGYALAPKKRHSFIQDSLVALAASRGIDLVRIDTDRPLLDQGPFDCILHKFYG 60

Query: 61  EDWRKQLVEFRAKNPNAFILDSPDAIERLHNRISMLQVVSELKIDNPDESFGIPKQIVIY 120
           EDWRKQL+EFR KNPNAFILDSPD+IERLHNRISMLQVVSELKIDNPDESFGIPKQIVIY
Sbjct: 61  EDWRKQLMEFRVKNPNAFILDSPDSIERLHNRISMLQVVSELKIDNPDESFGIPKQIVIY 120

Query: 121 DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG 180
           DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG
Sbjct: 121 DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG 180

Query: 181 VIFKVYVAGQYVKCVKRKSLPDEPEEKLGNVEGLLSFSQVSNLTTREKTDDKYYKMMQLD 240
           VIFKVYV GQYVKCVKRKSLPDEPE KLGNV+GLLSFSQVSN+T REK DDK+Y MMQLD
Sbjct: 181 VIFKVYVVGQYVKCVKRKSLPDEPEAKLGNVDGLLSFSQVSNMTPREKIDDKHY-MMQLD 240

Query: 241 DTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV 300
           DTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV
Sbjct: 241 DTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV 300

Query: 301 LTDFFCDLAQKKEAVKN-----------------SEQETRKIASDEDDGGLSDDRENKET 346
           LTDFFCDLAQKKEA+ N                 S+QETRKI +DE  GG S +RE KE 
Sbjct: 301 LTDFFCDLAQKKEALNNPDKKKDVEDKIVLDLQSSDQETRKIGNDEIGGGQSVEREKKEA 360

BLAST of MC10g1331 vs. NCBI nr
Match: XP_004148764.1 (inositol-tetrakisphosphate 1-kinase 1 [Cucumis sativus] >KGN55739.1 hypothetical protein Csa_011482 [Cucumis sativus])

HSP 1 Score: 626 bits (1614), Expect = 8.84e-225
Identity = 315/363 (86.78%), Postives = 329/363 (90.63%), Query Frame = 0

Query: 1   MADRRFCVGYALAPKKQQSFIQDSLVSLAASRGVDLVRIDTDRPLLDQGPFDCILHKFYG 60
           M  RRFC+GYALAPKK+ SFIQDSLV+LAASRGVDLVRIDTDRPLLDQGPFDCILHKFYG
Sbjct: 1   MEGRRFCIGYALAPKKRHSFIQDSLVTLAASRGVDLVRIDTDRPLLDQGPFDCILHKFYG 60

Query: 61  EDWRKQLVEFRAKNPNAFILDSPDAIERLHNRISMLQVVSELKIDNPDESFGIPKQIVIY 120
           EDWRKQL+EFR KNPNAFILDSPD+IERLHNRISMLQVVSELKIDNPDESFGIPKQIVIY
Sbjct: 61  EDWRKQLMEFRVKNPNAFILDSPDSIERLHNRISMLQVVSELKIDNPDESFGIPKQIVIY 120

Query: 121 DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG 180
           DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG
Sbjct: 121 DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG 180

Query: 181 VIFKVYVAGQYVKCVKRKSLPDEPEEKLGNVEGLLSFSQVSNLTTREKTDDKYYKMMQLD 240
           VIFKVYV GQYVKCVKRKSLPDEPE KLGNV+GLLSFSQVSN+T REK DDK+Y MMQLD
Sbjct: 181 VIFKVYVVGQYVKCVKRKSLPDEPEAKLGNVDGLLSFSQVSNMTPREKIDDKHY-MMQLD 240

Query: 241 DTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV 300
           DTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV
Sbjct: 241 DTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV 300

Query: 301 LTDFFCDLAQKKEAVKN-----------------SEQETRKIASDEDDGGLSDDRENKET 346
           LTDFFCDLAQKKEA+ N                 ++Q+TRKI +DE  GG S +RE K T
Sbjct: 301 LTDFFCDLAQKKEALNNPDKKKDVEDKIVLDLQSTDQQTRKIGNDEIGGGQSVEREKKGT 360

BLAST of MC10g1331 vs. NCBI nr
Match: KAG7028266.1 (Inositol-tetrakisphosphate 1-kinase 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 625 bits (1612), Expect = 2.23e-224
Identity = 314/368 (85.33%), Postives = 331/368 (89.95%), Query Frame = 0

Query: 1   MADRRFCVGYALAPKKQQSFIQDSLVSLAASRGVDLVRIDTDRPLLDQGPFDCILHKFYG 60
           MA+RRFC+GYALAPKKQQSFIQDSLVSLAASRG+DLVRIDTDRPLLDQGPFDCILHK+YG
Sbjct: 1   MAERRFCIGYALAPKKQQSFIQDSLVSLAASRGIDLVRIDTDRPLLDQGPFDCILHKYYG 60

Query: 61  EDWRKQLVEFRAKNPNAFILDSPDAIERLHNRISMLQVVSELKIDNPDESFGIPKQIVIY 120
           EDWRKQL+EFR KNPNA ILDSPD+IERLHNRISMLQVVSELKIDN +ESFGIPKQIVIY
Sbjct: 61  EDWRKQLIEFRVKNPNALILDSPDSIERLHNRISMLQVVSELKIDNANESFGIPKQIVIY 120

Query: 121 DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG 180
           DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG
Sbjct: 121 DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG 180

Query: 181 VIFKVYVAGQYVKCVKRKSLPDEPEEKLGNVEGLLSFSQVSNLTTREKTDDKYYKMMQLD 240
           +IFKVYV GQYVKCVKRKSLPDEPE KLGNVEGLLSFSQVSNLT REK +DK+YKMMQLD
Sbjct: 181 IIFKVYVVGQYVKCVKRKSLPDEPEAKLGNVEGLLSFSQVSNLTPREKINDKFYKMMQLD 240

Query: 241 DTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV 300
           DTEMPPLSFVTDIARGLRRS+NLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV
Sbjct: 241 DTEMPPLSFVTDIARGLRRSLNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV 300

Query: 301 LTDFFCDLAQKKEAVKNS---------------------EQETRKIASDEDDGGLSDDRE 346
           LTDFFCDLAQ K+ +K +                     +QETRK A DEDDGG S +RE
Sbjct: 301 LTDFFCDLAQNKDPMKKNPDTKEGEDKIGSDQQLPVQTADQETRKTAIDEDDGGQSVNRE 360

BLAST of MC10g1331 vs. ExPASy TrEMBL
Match: A0A6J1CUF6 (Inositol-tetrakisphosphate 1-kinase OS=Momordica charantia OX=3673 GN=LOC111014868 PE=3 SV=1)

HSP 1 Score: 695 bits (1794), Expect = 9.37e-253
Identity = 345/346 (99.71%), Postives = 345/346 (99.71%), Query Frame = 0

Query: 1   MADRRFCVGYALAPKKQQSFIQDSLVSLAASRGVDLVRIDTDRPLLDQGPFDCILHKFYG 60
           MADRRFCVGYALAPKKQQSFIQDSLVSLAASRGVDLVRIDTDRPLLDQGPFDCILHKFYG
Sbjct: 1   MADRRFCVGYALAPKKQQSFIQDSLVSLAASRGVDLVRIDTDRPLLDQGPFDCILHKFYG 60

Query: 61  EDWRKQLVEFRAKNPNAFILDSPDAIERLHNRISMLQVVSELKIDNPDESFGIPKQIVIY 120
           EDWRKQLVEFRAKNPNAFILDSPDAIERLHNRISMLQVVSELKIDNPDESFGIPKQIVIY
Sbjct: 61  EDWRKQLVEFRAKNPNAFILDSPDAIERLHNRISMLQVVSELKIDNPDESFGIPKQIVIY 120

Query: 121 DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG 180
           DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG
Sbjct: 121 DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG 180

Query: 181 VIFKVYVAGQYVKCVKRKSLPDEPEEKLGNVEGLLSFSQVSNLTTREKTDDKYYKMMQLD 240
           VIFKVYVAGQYVKCVKRKSLPDEPEEKLGNVEGLLSFSQVSNLTTREKTDDKYYKMMQLD
Sbjct: 181 VIFKVYVAGQYVKCVKRKSLPDEPEEKLGNVEGLLSFSQVSNLTTREKTDDKYYKMMQLD 240

Query: 241 DTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV 300
           DTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV
Sbjct: 241 DTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV 300

Query: 301 LTDFFCDLAQKKEAVKNSEQETRKIASDEDDGGLSDDRENKETPVQ 346
           LTDFFCDLAQKKEAVKNSEQETRKI SDEDDGGLSDDRENKETPVQ
Sbjct: 301 LTDFFCDLAQKKEAVKNSEQETRKIVSDEDDGGLSDDRENKETPVQ 346

BLAST of MC10g1331 vs. ExPASy TrEMBL
Match: A0A5A7UCU4 (Inositol-tetrakisphosphate 1-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G001470 PE=3 SV=1)

HSP 1 Score: 628 bits (1620), Expect = 5.22e-226
Identity = 315/363 (86.78%), Postives = 330/363 (90.91%), Query Frame = 0

Query: 1   MADRRFCVGYALAPKKQQSFIQDSLVSLAASRGVDLVRIDTDRPLLDQGPFDCILHKFYG 60
           M +RRFC+GYALAPKK+ SFIQDSLV+LAASRG+DLVRIDTDRPLLDQGPFDCILHKFYG
Sbjct: 1   MEERRFCIGYALAPKKRHSFIQDSLVALAASRGIDLVRIDTDRPLLDQGPFDCILHKFYG 60

Query: 61  EDWRKQLVEFRAKNPNAFILDSPDAIERLHNRISMLQVVSELKIDNPDESFGIPKQIVIY 120
           EDWRKQL+EFR KNPNAFILDSPD+IERLHNRISMLQVVSELKIDNPDESFGIPKQIVIY
Sbjct: 61  EDWRKQLMEFRVKNPNAFILDSPDSIERLHNRISMLQVVSELKIDNPDESFGIPKQIVIY 120

Query: 121 DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG 180
           DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG
Sbjct: 121 DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG 180

Query: 181 VIFKVYVAGQYVKCVKRKSLPDEPEEKLGNVEGLLSFSQVSNLTTREKTDDKYYKMMQLD 240
           VIFKVYV GQYVKCVKRKSLPDEPE KLGNV+GLLSFSQVSN+T REK DDK+Y MMQLD
Sbjct: 181 VIFKVYVVGQYVKCVKRKSLPDEPEAKLGNVDGLLSFSQVSNMTPREKIDDKHY-MMQLD 240

Query: 241 DTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV 300
           DTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV
Sbjct: 241 DTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV 300

Query: 301 LTDFFCDLAQKKEAVKN-----------------SEQETRKIASDEDDGGLSDDRENKET 346
           LTDFFCDLAQKKEA+ N                 S+QETRKI +DE  GG S +RE KE 
Sbjct: 301 LTDFFCDLAQKKEALNNPDKKKDVEDKIVLDLQSSDQETRKIGNDEIGGGQSVEREKKEA 360

BLAST of MC10g1331 vs. ExPASy TrEMBL
Match: A0A1S3BJM2 (Inositol-tetrakisphosphate 1-kinase OS=Cucumis melo OX=3656 GN=LOC103490768 PE=3 SV=1)

HSP 1 Score: 628 bits (1620), Expect = 5.22e-226
Identity = 315/363 (86.78%), Postives = 330/363 (90.91%), Query Frame = 0

Query: 1   MADRRFCVGYALAPKKQQSFIQDSLVSLAASRGVDLVRIDTDRPLLDQGPFDCILHKFYG 60
           M +RRFC+GYALAPKK+ SFIQDSLV+LAASRG+DLVRIDTDRPLLDQGPFDCILHKFYG
Sbjct: 1   MEERRFCIGYALAPKKRHSFIQDSLVALAASRGIDLVRIDTDRPLLDQGPFDCILHKFYG 60

Query: 61  EDWRKQLVEFRAKNPNAFILDSPDAIERLHNRISMLQVVSELKIDNPDESFGIPKQIVIY 120
           EDWRKQL+EFR KNPNAFILDSPD+IERLHNRISMLQVVSELKIDNPDESFGIPKQIVIY
Sbjct: 61  EDWRKQLMEFRVKNPNAFILDSPDSIERLHNRISMLQVVSELKIDNPDESFGIPKQIVIY 120

Query: 121 DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG 180
           DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG
Sbjct: 121 DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG 180

Query: 181 VIFKVYVAGQYVKCVKRKSLPDEPEEKLGNVEGLLSFSQVSNLTTREKTDDKYYKMMQLD 240
           VIFKVYV GQYVKCVKRKSLPDEPE KLGNV+GLLSFSQVSN+T REK DDK+Y MMQLD
Sbjct: 181 VIFKVYVVGQYVKCVKRKSLPDEPEAKLGNVDGLLSFSQVSNMTPREKIDDKHY-MMQLD 240

Query: 241 DTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV 300
           DTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV
Sbjct: 241 DTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV 300

Query: 301 LTDFFCDLAQKKEAVKN-----------------SEQETRKIASDEDDGGLSDDRENKET 346
           LTDFFCDLAQKKEA+ N                 S+QETRKI +DE  GG S +RE KE 
Sbjct: 301 LTDFFCDLAQKKEALNNPDKKKDVEDKIVLDLQSSDQETRKIGNDEIGGGQSVEREKKEA 360

BLAST of MC10g1331 vs. ExPASy TrEMBL
Match: A0A0A0L3W9 (Inositol-tetrakisphosphate 1-kinase OS=Cucumis sativus OX=3659 GN=Csa_3G009450 PE=3 SV=1)

HSP 1 Score: 626 bits (1614), Expect = 4.28e-225
Identity = 315/363 (86.78%), Postives = 329/363 (90.63%), Query Frame = 0

Query: 1   MADRRFCVGYALAPKKQQSFIQDSLVSLAASRGVDLVRIDTDRPLLDQGPFDCILHKFYG 60
           M  RRFC+GYALAPKK+ SFIQDSLV+LAASRGVDLVRIDTDRPLLDQGPFDCILHKFYG
Sbjct: 1   MEGRRFCIGYALAPKKRHSFIQDSLVTLAASRGVDLVRIDTDRPLLDQGPFDCILHKFYG 60

Query: 61  EDWRKQLVEFRAKNPNAFILDSPDAIERLHNRISMLQVVSELKIDNPDESFGIPKQIVIY 120
           EDWRKQL+EFR KNPNAFILDSPD+IERLHNRISMLQVVSELKIDNPDESFGIPKQIVIY
Sbjct: 61  EDWRKQLMEFRVKNPNAFILDSPDSIERLHNRISMLQVVSELKIDNPDESFGIPKQIVIY 120

Query: 121 DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG 180
           DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG
Sbjct: 121 DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG 180

Query: 181 VIFKVYVAGQYVKCVKRKSLPDEPEEKLGNVEGLLSFSQVSNLTTREKTDDKYYKMMQLD 240
           VIFKVYV GQYVKCVKRKSLPDEPE KLGNV+GLLSFSQVSN+T REK DDK+Y MMQLD
Sbjct: 181 VIFKVYVVGQYVKCVKRKSLPDEPEAKLGNVDGLLSFSQVSNMTPREKIDDKHY-MMQLD 240

Query: 241 DTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV 300
           DTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV
Sbjct: 241 DTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV 300

Query: 301 LTDFFCDLAQKKEAVKN-----------------SEQETRKIASDEDDGGLSDDRENKET 346
           LTDFFCDLAQKKEA+ N                 ++Q+TRKI +DE  GG S +RE K T
Sbjct: 301 LTDFFCDLAQKKEALNNPDKKKDVEDKIVLDLQSTDQQTRKIGNDEIGGGQSVEREKKGT 360

BLAST of MC10g1331 vs. ExPASy TrEMBL
Match: A0A6J1E6Y5 (Inositol-tetrakisphosphate 1-kinase OS=Cucurbita moschata OX=3662 GN=LOC111431242 PE=3 SV=1)

HSP 1 Score: 622 bits (1604), Expect = 1.78e-223
Identity = 314/368 (85.33%), Postives = 331/368 (89.95%), Query Frame = 0

Query: 1   MADRRFCVGYALAPKKQQSFIQDSLVSLAASRGVDLVRIDTDRPLLDQGPFDCILHKFYG 60
           MA+RRFC+GYALAPKKQQSFIQDSLVSLAASRG+DLVRIDTDRPLLDQGPFDCILHK+YG
Sbjct: 1   MAERRFCIGYALAPKKQQSFIQDSLVSLAASRGIDLVRIDTDRPLLDQGPFDCILHKYYG 60

Query: 61  EDWRKQLVEFRAKNPNAFILDSPDAIERLHNRISMLQVVSELKIDNPDESFGIPKQIVIY 120
           EDWRKQL+EFR KNPNA ILDSPD+IERLHNRISMLQVVSELKIDN +ESFGIPKQIVIY
Sbjct: 61  EDWRKQLIEFRVKNPNALILDSPDSIERLHNRISMLQVVSELKIDNANESFGIPKQIVIY 120

Query: 121 DKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG 180
           DKETLFDRQA EGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG
Sbjct: 121 DKETLFDRQACEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGG 180

Query: 181 VIFKVYVAGQYVKCVKRKSLPDEPEEKLGNVEGLLSFSQVSNLTTREKTDDKYYKMMQLD 240
           +IFKVYV GQYVKCVKRKSLPDEPE KLGNVEGLLSFSQVSNLT REK +DK+YKMMQLD
Sbjct: 181 IIFKVYVVGQYVKCVKRKSLPDEPEAKLGNVEGLLSFSQVSNLTPREKINDKFYKMMQLD 240

Query: 241 DTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV 300
           DTEMPPLSFVTDIARGLRRS+NLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV
Sbjct: 241 DTEMPPLSFVTDIARGLRRSLNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKV 300

Query: 301 LTDFFCDLAQKKEAVKNS---------------------EQETRKIASDEDDGGLSDDRE 346
           LTDFFCDLAQ K+++K S                     +QETRK A DEDDGG S +RE
Sbjct: 301 LTDFFCDLAQNKDSMKKSPDAKEGEDKIGSDRQLPVKTADQETRKTAIDEDDGGQSVNRE 360

BLAST of MC10g1331 vs. TAIR 10
Match: AT5G16760.1 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 380.6 bits (976), Expect = 1.4e-105
Identity = 186/312 (59.62%), Postives = 232/312 (74.36%), Query Frame = 0

Query: 5   RFCVGYALAPKKQQSFIQDSLVSLAASRGVDLVRIDTDRPLLDQGPFDCILHKFYGEDWR 64
           R+ VGYALA KKQ SFIQ SL+  +  RG+DLV++D  + LL+QG  DCI+HK Y   W+
Sbjct: 8   RYLVGYALAAKKQHSFIQPSLIEHSRQRGIDLVKLDPTKSLLEQGKLDCIIHKLYDVYWK 67

Query: 65  KQLVEFRAKNPNAFILDSPDAIERLHNRISMLQVVSELKIDNPD-ESFGIPKQIVIYDKE 124
           + L EFR K P   ++D P+AIERLHNR+SML+V+++L+    D E FG+P+Q+V+ D  
Sbjct: 68  ENLHEFREKCPGVPVIDLPEAIERLHNRVSMLEVITQLRFPVSDSERFGVPEQVVVMDSS 127

Query: 125 TLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGGVIF 184
            L    A   LKFPVIAKPL ADGSAKSHKM L+++ + +  LK PIVLQEFVNHGGVIF
Sbjct: 128 VLSGGGALGELKFPVIAKPLDADGSAKSHKMFLIYDQEGMKILKAPIVLQEFVNHGGVIF 187

Query: 185 KVYVAGQYVKCVKRKSLPDEPEEKLGNVEGLLSFSQVSNLTTREKTDDKYYKMMQLDDTE 244
           KVYV G +VKCVKR+SLPD  EEK+G  +G L FSQ+SNLT +E  + +Y +   L+  E
Sbjct: 188 KVYVVGDHVKCVKRRSLPDISEEKIGTSKGSLPFSQISNLTAQEDKNIEYGEDRSLEKVE 247

Query: 245 MPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKVLTD 304
           MPPLSF+TD+A+ +R SM LNLFNFDVIRD+K   RYLIIDINYFPGYAKMP YE VLT+
Sbjct: 248 MPPLSFLTDLAKAMRESMGLNLFNFDVIRDAKDANRYLIIDINYFPGYAKMPSYEPVLTE 307

Query: 305 FFCDLAQKKEAV 316
           FF D+  KK  V
Sbjct: 308 FFWDMVTKKNHV 319

BLAST of MC10g1331 vs. TAIR 10
Match: AT4G08170.2 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 280.8 bits (717), Expect = 1.5e-75
Identity = 140/306 (45.75%), Postives = 197/306 (64.38%), Query Frame = 0

Query: 8   VGYALAPKKQQSFIQDSLVSLAASRGVDLVRIDTDRPLLDQGPFDCILHKFYGEDWRKQL 67
           VGYAL  KK +SF+Q  L  LA ++G+  V ID ++PL +QGPFD +LHK  G++WR+ L
Sbjct: 43  VGYALTSKKIKSFLQPKLEGLARNKGILFVAIDQNKPLSEQGPFDIVLHKQIGKEWRRIL 102

Query: 68  VEFRAKNPNAFILDSPDAIERLHNRISMLQVVSELKIDNPDESFGIPKQIVI-YDKETLF 127
            EFR  +P+  +LD PDAI  L NR SMLQ V+++ + + +   G+PKQ+VI  D  ++ 
Sbjct: 103 EEFRLAHPDVTVLDPPDAILHLRNRQSMLQCVADMNLSDSNGRVGVPKQLVIKKDASSIP 162

Query: 128 DRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGGVIFKVY 187
           +     GL+ P++AKPLVADGSAKSH+++L ++   L KL+PP+VLQEFVNHGGV+FKVY
Sbjct: 163 EAVNNAGLRLPLVAKPLVADGSAKSHELSLAYDQHSLLKLEPPLVLQEFVNHGGVLFKVY 222

Query: 188 VAGQYVKCVKRKSLPDEPEEKLGNVEGLLSFSQVSNLTTREKTDDKYYKMMQLDDTEMPP 247
           + G+ ++ V+R SLPD    +L    G+  F +VS         D     +     E+PP
Sbjct: 223 IVGEAIRVVRRFSLPDVSRRELPKSAGVFRFPRVSCAAASADDAD-----LDPSIAELPP 282

Query: 248 LSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKVLTDFFC 307
              +  +A+ LRR + L LFN D+IR+     R+ +IDINYFPGY KMP YE V TDF  
Sbjct: 283 RPLLERLAKELRRGLGLRLFNLDIIREHGTRDRFYVIDINYFPGYGKMPEYEHVFTDFLL 342

Query: 308 DLAQKK 313
            + Q +
Sbjct: 343 SVVQSQ 343

BLAST of MC10g1331 vs. TAIR 10
Match: AT4G33770.1 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 269.6 bits (688), Expect = 3.4e-72
Identity = 133/310 (42.90%), Postives = 198/310 (63.87%), Query Frame = 0

Query: 4   RRFCVGYALAPKKQQSFIQDSLVSLAASRGVDLVRIDTDRPLLDQGPFDCILHKFYGEDW 63
           ++  VGYAL  KK++SF+Q  L  LA  +G+  V ID +RPL +QGPFD +LHK  G++W
Sbjct: 79  QKLVVGYALTSKKKKSFLQPKLELLARRKGIFFVAIDLNRPLSEQGPFDVVLHKLLGKEW 138

Query: 64  RKQLVEFRAKNPNAFILDSPDAIERLHNRISMLQVVSELKIDNPDESFGIPKQ-IVIYDK 123
            + + +++ K+P   +LD P +I+R++NR SMLQ +++LK+ +   S  +PKQ +V+ D 
Sbjct: 139 EEVIEDYQQKHPEVTVLDPPGSIQRIYNRQSMLQGMADLKLSDCSGSLFVPKQMVVLKDS 198

Query: 124 ETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNHGGVI 183
               D     GLKFP++AKPL  DG+AKSH++ L ++   L +L PP+VLQEFVNHGGV+
Sbjct: 199 AASADAVVEAGLKFPLVAKPLWIDGTAKSHQLYLAYDRRSLAELDPPLVLQEFVNHGGVM 258

Query: 184 FKVYVAGQYVKCVKRKSLPDEPEEKLGNVEGLLSFSQVSNLTTREKTDDKYYKMMQLDDT 243
           FKV+V G  +K ++R SLP+    +   V+G+  F +VS+        D   ++      
Sbjct: 259 FKVFVVGDVIKVMRRFSLPNVSNCEKAKVDGVFQFPRVSSAAASADNADLDPRV-----A 318

Query: 244 EMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYEKVLT 303
           E+PP  F+  + + LR  + L LFN D+IR+      + +IDINYFPGY K+P YE+V  
Sbjct: 319 ELPPKPFLEALVKELRSLLGLRLFNIDMIREHGSKNVFYVIDINYFPGYGKLPDYEQVFV 378

Query: 304 DFFCDLAQKK 313
           DFF +LAQ K
Sbjct: 379 DFFQNLAQVK 383

BLAST of MC10g1331 vs. TAIR 10
Match: AT4G33770.2 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 246.1 bits (627), Expect = 4.1e-65
Identity = 120/282 (42.55%), Postives = 180/282 (63.83%), Query Frame = 0

Query: 32  RGVDLVRIDTDRPLLDQGPFDCILHKFYGEDWRKQLVEFRAKNPNAFILDSPDAIERLHN 91
           +G+  V ID +RPL +QGPFD +LHK  G++W + + +++ K+P   +LD P +I+R++N
Sbjct: 14  KGIFFVAIDLNRPLSEQGPFDVVLHKLLGKEWEEVIEDYQQKHPEVTVLDPPGSIQRIYN 73

Query: 92  RISMLQVVSELKIDNPDESFGIPKQ-IVIYDKETLFDRQAWEGLKFPVIAKPLVADGSAK 151
           R SMLQ +++LK+ +   S  +PKQ +V+ D     D     GLKFP++AKPL  DG+AK
Sbjct: 74  RQSMLQGMADLKLSDCSGSLFVPKQMVVLKDSAASADAVVEAGLKFPLVAKPLWIDGTAK 133

Query: 152 SHKMALVFNHDCLNKLKPPIVLQEFVNHGGVIFKVYVAGQYVKCVKRKSLPDEPEEKLGN 211
           SH++ L ++   L +L PP+VLQEFVNHGGV+FKV+V G  +K ++R SLP+    +   
Sbjct: 134 SHQLYLAYDRRSLAELDPPLVLQEFVNHGGVMFKVFVVGDVIKVMRRFSLPNVSNCEKAK 193

Query: 212 VEGLLSFSQVSNLTTREKTDDKYYKMMQLDDTEMPPLSFVTDIARGLRRSMNLNLFNFDV 271
           V+G+  F +VS+        D   ++      E+PP  F+  + + LR  + L LFN D+
Sbjct: 194 VDGVFQFPRVSSAAASADNADLDPRV-----AELPPKPFLEALVKELRSLLGLRLFNIDM 253

Query: 272 IRDSKIGTRYLIIDINYFPGYAKMPGYEKVLTDFFCDLAQKK 313
           IR+      + +IDINYFPGY K+P YE+V  DFF +LAQ K
Sbjct: 254 IREHGSKNVFYVIDINYFPGYGKLPDYEQVFVDFFQNLAQVK 290

BLAST of MC10g1331 vs. TAIR 10
Match: AT4G08170.1 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 226.5 bits (576), Expect = 3.3e-59
Identity = 113/254 (44.49%), Postives = 161/254 (63.39%), Query Frame = 0

Query: 60  GEDWRKQLVEFRAKNPNAFILDSPDAIERLHNRISMLQVVSELKIDNPDESFGIPKQIVI 119
           G++WR+ L EFR  +P+  +LD PDAI  L NR SMLQ V+++ + + +   G+PKQ+VI
Sbjct: 7   GKEWRRILEEFRLAHPDVTVLDPPDAILHLRNRQSMLQCVADMNLSDSNGRVGVPKQLVI 66

Query: 120 -YDKETLFDRQAWEGLKFPVIAKPLVADGSAKSHKMALVFNHDCLNKLKPPIVLQEFVNH 179
             D  ++ +     GL+ P++AKPLVADGSAKSH+++L ++   L KL+PP+VLQEFVNH
Sbjct: 67  KKDASSIPEAVNNAGLRLPLVAKPLVADGSAKSHELSLAYDQHSLLKLEPPLVLQEFVNH 126

Query: 180 GGVIFKVYVAGQYVKCVKRKSLPDEPEEKLGNVEGLLSFSQVSNLTTREKTDDKYYKMMQ 239
           GGV+FKVY+ G+ ++ V+R SLPD    +L    G+  F +VS         D     + 
Sbjct: 127 GGVLFKVYIVGEAIRVVRRFSLPDVSRRELPKSAGVFRFPRVSCAAASADDAD-----LD 186

Query: 240 LDDTEMPPLSFVTDIARGLRRSMNLNLFNFDVIRDSKIGTRYLIIDINYFPGYAKMPGYE 299
               E+PP   +  +A+ LRR + L LFN D+IR+     R+ +IDINYFPGY KMP YE
Sbjct: 187 PSIAELPPRPLLERLAKELRRGLGLRLFNLDIIREHGTRDRFYVIDINYFPGYGKMPEYE 246

Query: 300 KVLTDFFCDLAQKK 313
            V TDF   + Q +
Sbjct: 247 HVFTDFLLSVVQSQ 255

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84Y012.2e-10861.01Inositol-tetrakisphosphate 1-kinase 1 OS=Zea mays OX=4577 GN=ITPK1 PE=2 SV=1[more]
A0JJZ61.1e-10760.82Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. indica OX=39946 GN=... [more]
Q7XBW01.1e-10760.82Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q9SBA51.9e-10459.62Inositol-tetrakisphosphate 1-kinase 1 OS=Arabidopsis thaliana OX=3702 GN=ITPK1 P... [more]
A2X4M88.5e-7649.84Inositol-tetrakisphosphate 1-kinase 4 OS=Oryza sativa subsp. indica OX=39946 GN=... [more]
Match NameE-valueIdentityDescription
XP_022145419.11.93e-25299.71inositol-tetrakisphosphate 1-kinase 1-like isoform X1 [Momordica charantia] >XP_... [more]
XP_038904771.18.70e-23189.11inositol-tetrakisphosphate 1-kinase 1-like [Benincasa hispida][more]
XP_008448667.11.08e-22586.78PREDICTED: inositol-tetrakisphosphate 1-kinase 1-like [Cucumis melo] >KAA0053030... [more]
XP_004148764.18.84e-22586.78inositol-tetrakisphosphate 1-kinase 1 [Cucumis sativus] >KGN55739.1 hypothetical... [more]
KAG7028266.12.23e-22485.33Inositol-tetrakisphosphate 1-kinase 5, partial [Cucurbita argyrosperma subsp. ar... [more]
Match NameE-valueIdentityDescription
A0A6J1CUF69.37e-25399.71Inositol-tetrakisphosphate 1-kinase OS=Momordica charantia OX=3673 GN=LOC1110148... [more]
A0A5A7UCU45.22e-22686.78Inositol-tetrakisphosphate 1-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A1S3BJM25.22e-22686.78Inositol-tetrakisphosphate 1-kinase OS=Cucumis melo OX=3656 GN=LOC103490768 PE=3... [more]
A0A0A0L3W94.28e-22586.78Inositol-tetrakisphosphate 1-kinase OS=Cucumis sativus OX=3659 GN=Csa_3G009450 P... [more]
A0A6J1E6Y51.78e-22385.33Inositol-tetrakisphosphate 1-kinase OS=Cucurbita moschata OX=3662 GN=LOC11143124... [more]
Match NameE-valueIdentityDescription
AT5G16760.11.4e-10559.62Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
AT4G08170.21.5e-7545.75Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
AT4G33770.13.4e-7242.90Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
AT4G33770.24.1e-6542.55Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
AT4G08170.13.3e-5944.49Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008656Inositol-tetrakisphosphate 1-kinasePIRSFPIRSF038186ITPKcoord: 1..321
e-value: 4.7E-129
score: 427.2
IPR008656Inositol-tetrakisphosphate 1-kinasePANTHERPTHR14217INOSITOL-TETRAKISPHOSPHATE 1-KINASEcoord: 2..325
IPR041429Inositol-tetrakisphosphate 1-kinase, N-terminalPFAMPF17927Ins134_P3_kin_Ncoord: 8..86
e-value: 4.1E-31
score: 107.2
IPR040464Inositol 1,3,4-trisphosphate 5/6-kinase, ATP-grasp domainPFAMPF05770Ins134_P3_kincoord: 108..306
e-value: 2.0E-81
score: 272.3
NoneNo IPR availableGENE3D3.30.470.20coord: 84..290
e-value: 1.7E-63
score: 216.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 315..346
NoneNo IPR availablePANTHERPTHR14217:SF17INOSITOL-TETRAKISPHOSPHATE 1-KINASEcoord: 2..325
NoneNo IPR availableSUPERFAMILY56059Glutathione synthetase ATP-binding domain-likecoord: 81..302

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g1331.1MC10g1331.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0052746 inositol phosphorylation
biological_process GO:0032957 inositol trisphosphate metabolic process
molecular_function GO:0005524 ATP binding
molecular_function GO:0052726 inositol-1,3,4-trisphosphate 5-kinase activity
molecular_function GO:0052725 inositol-1,3,4-trisphosphate 6-kinase activity
molecular_function GO:0047325 inositol tetrakisphosphate 1-kinase activity
molecular_function GO:0000287 magnesium ion binding