MC10g1248 (gene) Bitter gourd (Dali-11) v1

BLAST of MC10g1248 vs. ExPASy Swiss-Prot
Match: Q9FGL0 (WAT1-related protein At5g47470 OS=Arabidopsis thaliana OX=3702 GN=At5g47470 PE=3 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 7.5e-109
Identity = 207/347 (59.65%), Postives = 268/347 (77.23%), Query Frame = 0

Query: 1   MEDLAIMGGLVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSK 60
           +E++ I+GGLV VQFVYAGNS+LMS+ MS+G+ P T+VIFSTF+TF+I+SP A+ FER +
Sbjct: 25  IEEMVIVGGLVMVQFVYAGNSLLMSYLMSLGLGPFTIVIFSTFATFIILSPFAILFERKQ 84

Query: 61  WPNKLTLKLISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRL 120
           WPN+L+L+LI +L+LISF GVTLFQSLFL+GI+LTSP++ATAMPNLAPGLIF IAWI  L
Sbjct: 85  WPNELSLRLIGKLVLISFAGVTLFQSLFLEGIRLTSPAMATAMPNLAPGLIFFIAWIVGL 144

Query: 121 EKVELSCIYSKIKILGTLLCVVGAFTMTIMHSAS----KVDSTILPLFDEQDQILDRQKI 180
           EK+ L C+YSK+KILGTLLCV GA  M++MHS S    + D T  P+F     + DR K+
Sbjct: 145 EKMNLKCVYSKLKILGTLLCVFGALAMSVMHSTSISHKEEDDT--PIF-----VFDRDKV 204

Query: 181 LGCLYLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVW 240
           +GC+YL  AV VLS+ VVLQA+TL +FPAP+SL AIT+L+GV+ T  V +++ +  +++ 
Sbjct: 205 VGCIYLLGAVFVLSTNVVLQASTLAEFPAPISLSAITALLGVLITTVVLLLQNRKTKVLA 264

Query: 241 PWFVSVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGE 300
              +S   +  Y V+ G+VSGACVSFNGWAMKKRGPV VSMFSP  TV S+  ++ TLGE
Sbjct: 265 SSLISFGNLVGYSVLAGAVSGACVSFNGWAMKKRGPVFVSMFSPFATVISVAFAVLTLGE 324

Query: 301 SINVGSLAGMFLMFTGLYFVLWAKRKESYGTRNGPESEFDIDKPLLT 344
           S+++GS+ GM LMF GLY VLWAK KE +      ESEFD  KPLL+
Sbjct: 325 SVSLGSVGGMVLMFVGLYLVLWAKGKEGFSEIESFESEFDSKKPLLS 364

BLAST of MC10g1248 vs. ExPASy Swiss-Prot
Match: F4JMI7 (WAT1-related protein At4g16620 OS=Arabidopsis thaliana OX=3702 GN=At4g16620 PE=2 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 4.9e-84
Identity = 169/351 (48.15%), Postives = 245/351 (69.80%), Query Frame = 0

Query: 3   DLAIMGGLVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSKWP 62
           +  I+GGL G Q +YAGNS L+S  +S+GIDPL +VI  TF++ L+++P+A   ER  WP
Sbjct: 9   EAGIIGGLAGAQVIYAGNSELLSQLLSLGIDPLLIVILCTFASVLLITPLAFLLERKLWP 68

Query: 63  NKLTLKLISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRLEK 122
             L+ KL  +L+L++  GVTLFQ LFL+G++ TS S+ATAMPNL P  IFVIAW   +EK
Sbjct: 69  RSLSFKLKIKLVLVALAGVTLFQGLFLEGMKHTSASMATAMPNLCPAFIFVIAWAAGMEK 128

Query: 123 VELSCIYSKIKILGTLLCVVGAFTMTIMHSASKVDSTI--LPLFDEQDQILDRQKILGCL 182
           V+LSC+YS++K+ GT+LCV+GA  M++MHS +   S++  +P+  + + ++D+ KILGCL
Sbjct: 129 VKLSCMYSRVKMGGTVLCVMGALIMSLMHSTTATLSSVKTIPIVPD-EVVVDKDKILGCL 188

Query: 183 YLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVWPWFV 242
           YL  A+  LSS +VLQA+ L +FPAP+S+ ++ SL+G + T+ +Q     ++E+     +
Sbjct: 189 YLLLAICGLSSSIVLQASILAEFPAPISMFSMVSLMGGITTVALQYALKGSMEMGSASVI 248

Query: 243 SVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGESINV 302
            +  +  Y ++GG VSG  +SFN W +K++GPV+VS+FSPI TV  +++S FT+ ES N+
Sbjct: 249 GLGHLVGYAILGGLVSGGGLSFNAWVIKRKGPVIVSLFSPIATVVCVVVSAFTMEESFNL 308

Query: 303 GSLAGMFLMFTGLYFVLWAKRKESYG-----TRNGPES----EFDIDKPLL 343
           GS AGM LMF GLYFVLWAK KE         ++  ES    EFD+ KPLL
Sbjct: 309 GSFAGMALMFGGLYFVLWAKGKEDCEEIDEMKQDDEESLLRTEFDLQKPLL 358

BLAST of MC10g1248 vs. ExPASy Swiss-Prot
Match: O80638 (WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 4.4e-32
Identity = 109/343 (31.78%), Postives = 171/343 (49.85%), Query Frame = 0

Query: 10  LVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSKWPNKLTLKL 69
           +V +QF YAG S++  F ++ G+ P  L  +      + ++P A + +R   P K+TL +
Sbjct: 12  VVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKIRP-KMTLSI 71

Query: 70  ISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRLEKVELSCIY 129
             ++LL+     T+ Q+L+  G++ TS +   AM N+ P   F++AWIFRLEKV +  I+
Sbjct: 72  FFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEKVNVKKIH 131

Query: 130 SKIKILGTLLCVVGAFTMTIMHSASKVDSTILPL---------FDEQDQILDRQKILGCL 189
           S+ KILGT++ V GA  MT+      V   ++PL          D  +  + +    G  
Sbjct: 132 SQAKILGTIVTVGGAMLMTV------VKGPLIPLPWANPHDIHQDSSNTGVKQDLTKGAS 191

Query: 190 YLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVWPWFV 249
            +    +  +  + LQA TL  +P  +SL A    +G + +  V +   +     W   +
Sbjct: 192 LIAIGCICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPSAWAIHL 251

Query: 250 SVKEMAAYCVIGGSV-SGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGESIN 309
             K +AA  V GG + SG      G  MK RGPV V+ F+P+  V   IL    L E + 
Sbjct: 252 DSKLLAA--VYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILAEVMF 311

Query: 310 VGSLAGMFLMFTGLYFVLWAKRKESYGTRNGPESEF-DIDKPL 342
           +G + G  ++  GLY VLW K K+       P S F D+DK L
Sbjct: 312 LGRILGAIVIVLGLYSVLWGKSKDE------PSSSFSDMDKEL 339

BLAST of MC10g1248 vs. ExPASy Swiss-Prot
Match: Q9FL41 (WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.4e-30
Identity = 97/333 (29.13%), Postives = 169/333 (50.75%), Query Frame = 0

Query: 10  LVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSKWPNKLTLKL 69
           ++ +QF YAG +++    ++ G+    LV++       +++P A +FER   P K+T  +
Sbjct: 22  MISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKAQP-KITFSI 81

Query: 70  ISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRLEKVELSCIY 129
             QL ++   G  + Q+ +  G++ TSP+ + AM N+ P + F++A +FR+E ++L  ++
Sbjct: 82  FMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRMEMLDLKKLW 141

Query: 130 SKIKILGTLLCVVGAFTMTI-------------MHSASKVDSTILPLFDEQDQILDRQKI 189
            + KI GT++ V GA  MTI             MH     DS+       ++   D++ +
Sbjct: 142 CQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQ---DSSHANTTSSKNSSSDKEFL 201

Query: 190 LGCLYLFTAVVVLSSIVVLQAATLGDFPA-PMSLCAITSLIGVMFTIGVQ-IVEYKTVEI 249
            G + L  A +  +S+ VLQA  L  +    +SL  +   IG +  + V  ++E+     
Sbjct: 202 KGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEHNPSAW 261

Query: 250 VWPWFVSVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTL 309
              W +++   A   ++  S+S       G  MKKRGPV  + FSP+  V   ++  F L
Sbjct: 262 RIGWDMNLLAAAYSGIVASSIS---YYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSFVL 321

Query: 310 GESINVGSLAGMFLMFTGLYFVLWAKRKESYGT 328
            E I +G + G  L+  GLY VLW K+KE+  T
Sbjct: 322 AEKIFLGGVIGAVLIVIGLYAVLWGKQKENQVT 347

BLAST of MC10g1248 vs. ExPASy Swiss-Prot
Match: Q9FNA5 (WAT1-related protein At5g13670 OS=Arabidopsis thaliana OX=3702 GN=At5g13670 PE=2 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 5.3e-30
Identity = 98/324 (30.25%), Postives = 170/324 (52.47%), Query Frame = 0

Query: 10  LVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSKWPNKLTLKL 69
           +V +Q +YA  S++    ++ G+ P  LV +       +++P A+  ER+  P KLT K+
Sbjct: 12  IVFIQCLYALMSIVAKLALNKGMSPHVLVAYRMAVASALITPFALILERNTRP-KLTFKI 71

Query: 70  ISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRLEKVELSCIY 129
           + Q+ ++S     + Q+L+  G++LT+ +  +A+ N  P + F++A +F+LEKV +   +
Sbjct: 72  LLQIAILSLFEPVVEQNLYYSGMKLTTATFTSALCNALPAMTFIMACVFKLEKVTIERRH 131

Query: 130 SKIKILGTLLCVVGAFTMT-----IMHSASKVDSTILPLFDEQDQILDRQKIL-GCLYLF 189
           S+ K++GT++ + GA  MT     ++      +S  L       +I  +  I  G + L 
Sbjct: 132 SQAKLVGTMVAIGGAMLMTFVKGNVIELPWTSNSRGLNGHTHAMRIPKQADIARGSIMLV 191

Query: 190 TAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVM-FTIGVQIVEYKTVEIVWPWFVSV 249
            +    S  ++LQA  L  + A +SL A+  ++G++  T+   I E K + +   W ++ 
Sbjct: 192 ASCFSWSCYIILQAKILAQYKAELSLTALMCIMGMLEATVMGLIWERKNMSV---WKINP 251

Query: 250 KEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGESINVGS 309
                  + GG VSG      GWA K+RGPV VS F+P+  V   ILS F   E + VG 
Sbjct: 252 DVTLLASIYGGLVSGLAYYVIGWASKERGPVFVSAFNPLSMVLVAILSTFVFLEKVYVGR 311

Query: 310 LAGMFLMFTGLYFVLWAKRKESYG 327
           + G  ++  G+Y VLW K K+  G
Sbjct: 312 VIGSVVIVIGIYLVLWGKSKDKGG 331

BLAST of MC10g1248 vs. NCBI nr
Match: XP_022154043.1 (WAT1-related protein At5g47470-like isoform X1 [Momordica charantia])

HSP 1 Score: 650 bits (1678), Expect = 7.09e-235
Identity = 343/343 (100.00%), Postives = 343/343 (100.00%), Query Frame = 0

Query: 1   MEDLAIMGGLVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSK 60
           MEDLAIMGGLVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSK
Sbjct: 1   MEDLAIMGGLVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSK 60

Query: 61  WPNKLTLKLISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRL 120
           WPNKLTLKLISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRL
Sbjct: 61  WPNKLTLKLISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRL 120

Query: 121 EKVELSCIYSKIKILGTLLCVVGAFTMTIMHSASKVDSTILPLFDEQDQILDRQKILGCL 180
           EKVELSCIYSKIKILGTLLCVVGAFTMTIMHSASKVDSTILPLFDEQDQILDRQKILGCL
Sbjct: 121 EKVELSCIYSKIKILGTLLCVVGAFTMTIMHSASKVDSTILPLFDEQDQILDRQKILGCL 180

Query: 181 YLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVWPWFV 240
           YLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVWPWFV
Sbjct: 181 YLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVWPWFV 240

Query: 241 SVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGESINV 300
           SVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGESINV
Sbjct: 241 SVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGESINV 300

Query: 301 GSLAGMFLMFTGLYFVLWAKRKESYGTRNGPESEFDIDKPLLT 343
           GSLAGMFLMFTGLYFVLWAKRKESYGTRNGPESEFDIDKPLLT
Sbjct: 301 GSLAGMFLMFTGLYFVLWAKRKESYGTRNGPESEFDIDKPLLT 343

BLAST of MC10g1248 vs. NCBI nr
Match: XP_022154044.1 (WAT1-related protein At5g47470-like isoform X2 [Momordica charantia])

HSP 1 Score: 628 bits (1619), Expect = 4.62e-226
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 0

Query: 1   MEDLAIMGGLVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSK 60
           MEDLAIMGGLVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSK
Sbjct: 1   MEDLAIMGGLVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSK 60

Query: 61  WPNKLTLKLISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRL 120
           WPNKLTLKLISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRL
Sbjct: 61  WPNKLTLKLISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRL 120

Query: 121 EKVELSCIYSKIKILGTLLCVVGAFTMTIMHSASKVDSTILPLFDEQDQILDRQKILGCL 180
           EKVELSCIYSKIKILGTLLCVVGAFTMTIMHSASKVDSTILPLFDEQDQILDRQKILGCL
Sbjct: 121 EKVELSCIYSKIKILGTLLCVVGAFTMTIMHSASKVDSTILPLFDEQDQILDRQKILGCL 180

Query: 181 YLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVWPWFV 240
           YLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVWPWFV
Sbjct: 181 YLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVWPWFV 240

Query: 241 SVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGESINV 300
           SVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGESINV
Sbjct: 241 SVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGESINV 300

Query: 301 GSLAGMFLMFTGLYFVLWAKRKESYGTRNGPE 332
           GSLAGMFLMFTGLYFVLWAKRKESYGTRNGPE
Sbjct: 301 GSLAGMFLMFTGLYFVLWAKRKESYGTRNGPE 332

BLAST of MC10g1248 vs. NCBI nr
Match: XP_038906245.1 (WAT1-related protein At5g47470 [Benincasa hispida])

HSP 1 Score: 519 bits (1337), Expect = 7.70e-183
Identity = 274/346 (79.19%), Postives = 304/346 (87.86%), Query Frame = 0

Query: 1   MEDLAIMGGLVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSK 60
           ME+ AIM GLVGVQFVYAGNSV MSF MS+GI+PLTLVIFSTFSTFLIVSPIAVYFER  
Sbjct: 6   MENFAIMSGLVGVQFVYAGNSVFMSFLMSLGINPLTLVIFSTFSTFLIVSPIAVYFERHD 65

Query: 61  WPNKLTLKLISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRL 120
           WP KL+LKLI QLLLISFGGVTLFQSLFLKGIQLTSP+LATAMPNL PGLIF IAWIFRL
Sbjct: 66  WPKKLSLKLILQLLLISFGGVTLFQSLFLKGIQLTSPALATAMPNLTPGLIFAIAWIFRL 125

Query: 121 EKVELSCIYSKIKILGTLLCVVGAFTMTIMHSASK---VDSTILPLFDEQDQILDRQKIL 180
           EKV+LSCIYSKIKILGT LCV+GAFTM+IMHSAS        I       +Q+ + QKIL
Sbjct: 126 EKVQLSCIYSKIKILGTFLCVIGAFTMSIMHSASSDAAAKDNIPRQSPPSEQVFNEQKIL 185

Query: 181 GCLYLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVWP 240
           GC+YLF+A++VLSSIVVLQA+TLGDFPAPMSLCAITSLIGV+ TI VQIV+Y+ ++I WP
Sbjct: 186 GCVYLFSAIIVLSSIVVLQASTLGDFPAPMSLCAITSLIGVLITIVVQIVQYQKLDIGWP 245

Query: 241 WFVSVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGES 300
             +S KE+AAY V+GG++SGACVSFNGWAMKKRGPVLVSMFSPIGTV SLILS+FTLGES
Sbjct: 246 LLISGKELAAYFVLGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLILSVFTLGES 305

Query: 301 INVGSLAGMFLMFTGLYFVLWAKRKESYGTRNGPESEFDIDKPLLT 343
           IN+GSLAGMFLMFTGLYFVLWAK KE Y  RN PESEFD+DKPLL+
Sbjct: 306 INIGSLAGMFLMFTGLYFVLWAKGKERYVNRNCPESEFDVDKPLLS 351

BLAST of MC10g1248 vs. NCBI nr
Match: XP_004147210.1 (WAT1-related protein At5g47470 isoform X1 [Cucumis sativus])

HSP 1 Score: 517 bits (1332), Expect = 4.44e-182
Identity = 271/346 (78.32%), Postives = 303/346 (87.57%), Query Frame = 0

Query: 1   MEDLAIMGGLVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSK 60
           MED AIM GLVGVQFVYAGNSV MSF MS+GIDPLTLVIFSTFSTFLIVSP+AVYFER  
Sbjct: 6   MEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYFERYN 65

Query: 61  WPNKLTLKLISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRL 120
           WP KL+LKLI QL+LISFGGVTLFQSL LKGIQLTSP+LATAMPNLAPGLIF IAWIFRL
Sbjct: 66  WPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAWIFRL 125

Query: 121 EKVELSCIYSKIKILGTLLCVVGAFTMTIMHSA-SKV--DSTILPLFDEQDQILDRQKIL 180
           EKV+LSCIYSKIKILGTLLCV+GAFTM+IMHSA S V   ++I       +Q+ D QKIL
Sbjct: 126 EKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFDEQKIL 185

Query: 181 GCLYLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVWP 240
           GC YLF A+++LS IVVLQAATLGDFPAPMSLCAITSLIGV+ TIGVQI++Y  ++I WP
Sbjct: 186 GCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRIDIAWP 245

Query: 241 WFVSVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGES 300
             + VKE+AAY ++GG++SGACVSFNGWAMKKRGPVLVSMFSPIGTV SL+LS+ TLGES
Sbjct: 246 LLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVITLGES 305

Query: 301 INVGSLAGMFLMFTGLYFVLWAKRKESYGTRNGPESEFDIDKPLLT 343
           IN+GSLAGMF+MFTGLYFVLWAK KE Y  RN  ESEFD+DKPLL+
Sbjct: 306 INIGSLAGMFMMFTGLYFVLWAKGKERYVNRNHLESEFDVDKPLLS 351

BLAST of MC10g1248 vs. NCBI nr
Match: KAA0041079.1 (WAT1-related protein [Cucumis melo var. makuwa] >TYK12029.1 WAT1-related protein [Cucumis melo var. makuwa])

HSP 1 Score: 516 bits (1329), Expect = 1.06e-181
Identity = 269/347 (77.52%), Postives = 304/347 (87.61%), Query Frame = 0

Query: 1   MEDLAIMGGLVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSK 60
           MED AIM GLVGVQFVYAGNSV MSF MS+GIDPLTLVIFSTFSTFLIVSP+AVYFER  
Sbjct: 1   MEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYFERYS 60

Query: 61  WPNKLTLKLISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRL 120
           WP KL+LKLI QL+LISFGGVTLFQSL LKGIQLTSP+LATAMPNLAPGLIF IAWIFRL
Sbjct: 61  WPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAWIFRL 120

Query: 121 EKVELSCIYSKIKILGTLLCVVGAFTMTIMHSASKVDSTILPLFDEQ----DQILDRQKI 180
           EKV+LSCIYSKIKILGTLLCV+GAFTM++MHSAS  D+        Q    + + D QKI
Sbjct: 121 EKVQLSCIYSKIKILGTLLCVIGAFTMSVMHSASS-DTAGKDSVPRQSPLSEHMFDEQKI 180

Query: 181 LGCLYLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVW 240
           LGC YLF+A+++LSS+VVLQA+TLGDFPAPMSLCAITSLIGV+ TIGVQIV+Y  ++I W
Sbjct: 181 LGCAYLFSAIIILSSVVVLQASTLGDFPAPMSLCAITSLIGVLLTIGVQIVQYHRIDIAW 240

Query: 241 PWFVSVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGE 300
           P  +SVKE+AAY ++GG++SGACVSFNGWAMKKRGPVLVSMFSPIGTV SL+LS+ TLGE
Sbjct: 241 PLLISVKELAAYVIVGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVVTLGE 300

Query: 301 SINVGSLAGMFLMFTGLYFVLWAKRKESYGTRNGPESEFDIDKPLLT 343
           SIN+GSLAGMF+MFTGLYFVLWAK KE Y + N  ESEFD+DKPLL+
Sbjct: 301 SINIGSLAGMFMMFTGLYFVLWAKGKERYVSLNHLESEFDVDKPLLS 346

BLAST of MC10g1248 vs. ExPASy TrEMBL
Match: A0A6J1DJ66 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111021397 PE=3 SV=1)

HSP 1 Score: 650 bits (1678), Expect = 3.43e-235
Identity = 343/343 (100.00%), Postives = 343/343 (100.00%), Query Frame = 0

Query: 1   MEDLAIMGGLVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSK 60
           MEDLAIMGGLVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSK
Sbjct: 1   MEDLAIMGGLVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSK 60

Query: 61  WPNKLTLKLISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRL 120
           WPNKLTLKLISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRL
Sbjct: 61  WPNKLTLKLISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRL 120

Query: 121 EKVELSCIYSKIKILGTLLCVVGAFTMTIMHSASKVDSTILPLFDEQDQILDRQKILGCL 180
           EKVELSCIYSKIKILGTLLCVVGAFTMTIMHSASKVDSTILPLFDEQDQILDRQKILGCL
Sbjct: 121 EKVELSCIYSKIKILGTLLCVVGAFTMTIMHSASKVDSTILPLFDEQDQILDRQKILGCL 180

Query: 181 YLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVWPWFV 240
           YLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVWPWFV
Sbjct: 181 YLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVWPWFV 240

Query: 241 SVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGESINV 300
           SVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGESINV
Sbjct: 241 SVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGESINV 300

Query: 301 GSLAGMFLMFTGLYFVLWAKRKESYGTRNGPESEFDIDKPLLT 343
           GSLAGMFLMFTGLYFVLWAKRKESYGTRNGPESEFDIDKPLLT
Sbjct: 301 GSLAGMFLMFTGLYFVLWAKRKESYGTRNGPESEFDIDKPLLT 343

BLAST of MC10g1248 vs. ExPASy TrEMBL
Match: A0A6J1DKW5 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111021397 PE=3 SV=1)

HSP 1 Score: 628 bits (1619), Expect = 2.23e-226
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 0

Query: 1   MEDLAIMGGLVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSK 60
           MEDLAIMGGLVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSK
Sbjct: 1   MEDLAIMGGLVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSK 60

Query: 61  WPNKLTLKLISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRL 120
           WPNKLTLKLISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRL
Sbjct: 61  WPNKLTLKLISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRL 120

Query: 121 EKVELSCIYSKIKILGTLLCVVGAFTMTIMHSASKVDSTILPLFDEQDQILDRQKILGCL 180
           EKVELSCIYSKIKILGTLLCVVGAFTMTIMHSASKVDSTILPLFDEQDQILDRQKILGCL
Sbjct: 121 EKVELSCIYSKIKILGTLLCVVGAFTMTIMHSASKVDSTILPLFDEQDQILDRQKILGCL 180

Query: 181 YLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVWPWFV 240
           YLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVWPWFV
Sbjct: 181 YLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVWPWFV 240

Query: 241 SVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGESINV 300
           SVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGESINV
Sbjct: 241 SVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGESINV 300

Query: 301 GSLAGMFLMFTGLYFVLWAKRKESYGTRNGPE 332
           GSLAGMFLMFTGLYFVLWAKRKESYGTRNGPE
Sbjct: 301 GSLAGMFLMFTGLYFVLWAKRKESYGTRNGPE 332

BLAST of MC10g1248 vs. ExPASy TrEMBL
Match: A0A5D3CP19 (WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1017G00410 PE=3 SV=1)

HSP 1 Score: 516 bits (1329), Expect = 5.14e-182
Identity = 269/347 (77.52%), Postives = 304/347 (87.61%), Query Frame = 0

Query: 1   MEDLAIMGGLVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSK 60
           MED AIM GLVGVQFVYAGNSV MSF MS+GIDPLTLVIFSTFSTFLIVSP+AVYFER  
Sbjct: 1   MEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYFERYS 60

Query: 61  WPNKLTLKLISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRL 120
           WP KL+LKLI QL+LISFGGVTLFQSL LKGIQLTSP+LATAMPNLAPGLIF IAWIFRL
Sbjct: 61  WPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAWIFRL 120

Query: 121 EKVELSCIYSKIKILGTLLCVVGAFTMTIMHSASKVDSTILPLFDEQ----DQILDRQKI 180
           EKV+LSCIYSKIKILGTLLCV+GAFTM++MHSAS  D+        Q    + + D QKI
Sbjct: 121 EKVQLSCIYSKIKILGTLLCVIGAFTMSVMHSASS-DTAGKDSVPRQSPLSEHMFDEQKI 180

Query: 181 LGCLYLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVW 240
           LGC YLF+A+++LSS+VVLQA+TLGDFPAPMSLCAITSLIGV+ TIGVQIV+Y  ++I W
Sbjct: 181 LGCAYLFSAIIILSSVVVLQASTLGDFPAPMSLCAITSLIGVLLTIGVQIVQYHRIDIAW 240

Query: 241 PWFVSVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGE 300
           P  +SVKE+AAY ++GG++SGACVSFNGWAMKKRGPVLVSMFSPIGTV SL+LS+ TLGE
Sbjct: 241 PLLISVKELAAYVIVGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVVTLGE 300

Query: 301 SINVGSLAGMFLMFTGLYFVLWAKRKESYGTRNGPESEFDIDKPLLT 343
           SIN+GSLAGMF+MFTGLYFVLWAK KE Y + N  ESEFD+DKPLL+
Sbjct: 301 SINIGSLAGMFMMFTGLYFVLWAKGKERYVSLNHLESEFDVDKPLLS 346

BLAST of MC10g1248 vs. ExPASy TrEMBL
Match: A0A1S3BKN0 (WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103490881 PE=3 SV=1)

HSP 1 Score: 516 bits (1329), Expect = 9.16e-182
Identity = 269/347 (77.52%), Postives = 304/347 (87.61%), Query Frame = 0

Query: 1   MEDLAIMGGLVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSK 60
           MED AIM GLVGVQFVYAGNSV MSF MS+GIDPLTLVIFSTFSTFLIVSP+AVYFER  
Sbjct: 17  MEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYFERYS 76

Query: 61  WPNKLTLKLISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRL 120
           WP KL+LKLI QL+LISFGGVTLFQSL LKGIQLTSP+LATAMPNLAPGLIF IAWIFRL
Sbjct: 77  WPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAWIFRL 136

Query: 121 EKVELSCIYSKIKILGTLLCVVGAFTMTIMHSASKVDSTILPLFDEQ----DQILDRQKI 180
           EKV+LSCIYSKIKILGTLLCV+GAFTM++MHSAS  D+        Q    + + D QKI
Sbjct: 137 EKVQLSCIYSKIKILGTLLCVIGAFTMSVMHSASS-DTAGKDSVPRQSPLSEHMFDEQKI 196

Query: 181 LGCLYLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVW 240
           LGC YLF+A+++LSS+VVLQA+TLGDFPAPMSLCAITSLIGV+ TIGVQIV+Y  ++I W
Sbjct: 197 LGCAYLFSAIIILSSVVVLQASTLGDFPAPMSLCAITSLIGVLLTIGVQIVQYHRIDIAW 256

Query: 241 PWFVSVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGE 300
           P  +SVKE+AAY ++GG++SGACVSFNGWAMKKRGPVLVSMFSPIGTV SL+LS+ TLGE
Sbjct: 257 PLLISVKELAAYVIVGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVVTLGE 316

Query: 301 SINVGSLAGMFLMFTGLYFVLWAKRKESYGTRNGPESEFDIDKPLLT 343
           SIN+GSLAGMF+MFTGLYFVLWAK KE Y + N  ESEFD+DKPLL+
Sbjct: 317 SINIGSLAGMFMMFTGLYFVLWAKGKERYVSLNHLESEFDVDKPLLS 362

BLAST of MC10g1248 vs. ExPASy TrEMBL
Match: A0A6J1L2K3 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111498520 PE=3 SV=1)

HSP 1 Score: 499 bits (1285), Expect = 2.13e-175
Identity = 262/343 (76.38%), Postives = 295/343 (86.01%), Query Frame = 0

Query: 1   MEDLAIMGGLVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSK 60
           MED  IM GLV VQFVYAGNSV MSF MS+ IDPLTL+IF+TF TFLIVSP+A+YFERS 
Sbjct: 1   MEDFGIMSGLVAVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVALYFERSN 60

Query: 61  WPNKLTLKLISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRL 120
           WP KL+LKLISQ+LLISFGGVTLFQSLFLKGIQLTSPS+ATAMPNLAPGLIFVIAWIFRL
Sbjct: 61  WPTKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL 120

Query: 121 EKVELSCIYSKIKILGTLLCVVGAFTMTIMHSASKVDSTILPLFDEQDQILDRQKILGCL 180
           EKV LSCIYSKIKILGT LCV+GAFTM+IMHS    D+         DQ  D QKI+GC+
Sbjct: 121 EKVRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLS-DAAANVGRRPADQAFDEQKIVGCV 180

Query: 181 YLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVWPWFV 240
           YL +AVVVLSS+VVLQA+TLGDFP PMSLCAITSL+GV+ TI + I  Y+ +EI W  ++
Sbjct: 181 YLISAVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITIVIHIALYQKLEIGWS-YL 240

Query: 241 SVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGESINV 300
           SV+++ AYCV+ G+VSGACVSFNGWAMKKRGPVLVSMFSPIGTV SL+LS+FTLGE IN+
Sbjct: 241 SVEKLIAYCVLSGTVSGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVFTLGEPINI 300

Query: 301 GSLAGMFLMFTGLYFVLWAKRKESYGTRNGPESEFDIDKPLLT 343
           GSL GMFLMFTGLYFVLWAKRKE Y  RN PESEFDID+PLL+
Sbjct: 301 GSLVGMFLMFTGLYFVLWAKRKEGYANRNRPESEFDIDEPLLS 341

BLAST of MC10g1248 vs. TAIR 10
Match: AT5G47470.1 (Nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 395.2 bits (1014), Expect = 5.4e-110
Identity = 207/347 (59.65%), Postives = 268/347 (77.23%), Query Frame = 0

Query: 1   MEDLAIMGGLVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSK 60
           +E++ I+GGLV VQFVYAGNS+LMS+ MS+G+ P T+VIFSTF+TF+I+SP A+ FER +
Sbjct: 25  IEEMVIVGGLVMVQFVYAGNSLLMSYLMSLGLGPFTIVIFSTFATFIILSPFAILFERKQ 84

Query: 61  WPNKLTLKLISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRL 120
           WPN+L+L+LI +L+LISF GVTLFQSLFL+GI+LTSP++ATAMPNLAPGLIF IAWI  L
Sbjct: 85  WPNELSLRLIGKLVLISFAGVTLFQSLFLEGIRLTSPAMATAMPNLAPGLIFFIAWIVGL 144

Query: 121 EKVELSCIYSKIKILGTLLCVVGAFTMTIMHSAS----KVDSTILPLFDEQDQILDRQKI 180
           EK+ L C+YSK+KILGTLLCV GA  M++MHS S    + D T  P+F     + DR K+
Sbjct: 145 EKMNLKCVYSKLKILGTLLCVFGALAMSVMHSTSISHKEEDDT--PIF-----VFDRDKV 204

Query: 181 LGCLYLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVW 240
           +GC+YL  AV VLS+ VVLQA+TL +FPAP+SL AIT+L+GV+ T  V +++ +  +++ 
Sbjct: 205 VGCIYLLGAVFVLSTNVVLQASTLAEFPAPISLSAITALLGVLITTVVLLLQNRKTKVLA 264

Query: 241 PWFVSVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGE 300
              +S   +  Y V+ G+VSGACVSFNGWAMKKRGPV VSMFSP  TV S+  ++ TLGE
Sbjct: 265 SSLISFGNLVGYSVLAGAVSGACVSFNGWAMKKRGPVFVSMFSPFATVISVAFAVLTLGE 324

Query: 301 SINVGSLAGMFLMFTGLYFVLWAKRKESYGTRNGPESEFDIDKPLLT 344
           S+++GS+ GM LMF GLY VLWAK KE +      ESEFD  KPLL+
Sbjct: 325 SVSLGSVGGMVLMFVGLYLVLWAKGKEGFSEIESFESEFDSKKPLLS 364

BLAST of MC10g1248 vs. TAIR 10
Match: AT4G16620.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 312.8 bits (800), Expect = 3.5e-85
Identity = 169/351 (48.15%), Postives = 245/351 (69.80%), Query Frame = 0

Query: 3   DLAIMGGLVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSKWP 62
           +  I+GGL G Q +YAGNS L+S  +S+GIDPL +VI  TF++ L+++P+A   ER  WP
Sbjct: 9   EAGIIGGLAGAQVIYAGNSELLSQLLSLGIDPLLIVILCTFASVLLITPLAFLLERKLWP 68

Query: 63  NKLTLKLISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRLEK 122
             L+ KL  +L+L++  GVTLFQ LFL+G++ TS S+ATAMPNL P  IFVIAW   +EK
Sbjct: 69  RSLSFKLKIKLVLVALAGVTLFQGLFLEGMKHTSASMATAMPNLCPAFIFVIAWAAGMEK 128

Query: 123 VELSCIYSKIKILGTLLCVVGAFTMTIMHSASKVDSTI--LPLFDEQDQILDRQKILGCL 182
           V+LSC+YS++K+ GT+LCV+GA  M++MHS +   S++  +P+  + + ++D+ KILGCL
Sbjct: 129 VKLSCMYSRVKMGGTVLCVMGALIMSLMHSTTATLSSVKTIPIVPD-EVVVDKDKILGCL 188

Query: 183 YLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVWPWFV 242
           YL  A+  LSS +VLQA+ L +FPAP+S+ ++ SL+G + T+ +Q     ++E+     +
Sbjct: 189 YLLLAICGLSSSIVLQASILAEFPAPISMFSMVSLMGGITTVALQYALKGSMEMGSASVI 248

Query: 243 SVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGESINV 302
            +  +  Y ++GG VSG  +SFN W +K++GPV+VS+FSPI TV  +++S FT+ ES N+
Sbjct: 249 GLGHLVGYAILGGLVSGGGLSFNAWVIKRKGPVIVSLFSPIATVVCVVVSAFTMEESFNL 308

Query: 303 GSLAGMFLMFTGLYFVLWAKRKESYG-----TRNGPES----EFDIDKPLL 343
           GS AGM LMF GLYFVLWAK KE         ++  ES    EFD+ KPLL
Sbjct: 309 GSFAGMALMFGGLYFVLWAKGKEDCEEIDEMKQDDEESLLRTEFDLQKPLL 358

BLAST of MC10g1248 vs. TAIR 10
Match: AT2G39510.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 140.2 bits (352), Expect = 3.1e-33
Identity = 109/343 (31.78%), Postives = 171/343 (49.85%), Query Frame = 0

Query: 10  LVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSKWPNKLTLKL 69
           +V +QF YAG S++  F ++ G+ P  L  +      + ++P A + +R   P K+TL +
Sbjct: 12  VVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKIRP-KMTLSI 71

Query: 70  ISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRLEKVELSCIY 129
             ++LL+     T+ Q+L+  G++ TS +   AM N+ P   F++AWIFRLEKV +  I+
Sbjct: 72  FFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEKVNVKKIH 131

Query: 130 SKIKILGTLLCVVGAFTMTIMHSASKVDSTILPL---------FDEQDQILDRQKILGCL 189
           S+ KILGT++ V GA  MT+      V   ++PL          D  +  + +    G  
Sbjct: 132 SQAKILGTIVTVGGAMLMTV------VKGPLIPLPWANPHDIHQDSSNTGVKQDLTKGAS 191

Query: 190 YLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVWPWFV 249
            +    +  +  + LQA TL  +P  +SL A    +G + +  V +   +     W   +
Sbjct: 192 LIAIGCICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPSAWAIHL 251

Query: 250 SVKEMAAYCVIGGSV-SGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGESIN 309
             K +AA  V GG + SG      G  MK RGPV V+ F+P+  V   IL    L E + 
Sbjct: 252 DSKLLAA--VYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILAEVMF 311

Query: 310 VGSLAGMFLMFTGLYFVLWAKRKESYGTRNGPESEF-DIDKPL 342
           +G + G  ++  GLY VLW K K+       P S F D+DK L
Sbjct: 312 LGRILGAIVIVLGLYSVLWGKSKDE------PSSSFSDMDKEL 339

BLAST of MC10g1248 vs. TAIR 10
Match: AT5G07050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 135.2 bits (339), Expect = 1.0e-31
Identity = 97/333 (29.13%), Postives = 169/333 (50.75%), Query Frame = 0

Query: 10  LVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSKWPNKLTLKL 69
           ++ +QF YAG +++    ++ G+    LV++       +++P A +FER   P K+T  +
Sbjct: 22  MISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKAQP-KITFSI 81

Query: 70  ISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRLEKVELSCIY 129
             QL ++   G  + Q+ +  G++ TSP+ + AM N+ P + F++A +FR+E ++L  ++
Sbjct: 82  FMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRMEMLDLKKLW 141

Query: 130 SKIKILGTLLCVVGAFTMTI-------------MHSASKVDSTILPLFDEQDQILDRQKI 189
            + KI GT++ V GA  MTI             MH     DS+       ++   D++ +
Sbjct: 142 CQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQ---DSSHANTTSSKNSSSDKEFL 201

Query: 190 LGCLYLFTAVVVLSSIVVLQAATLGDFPA-PMSLCAITSLIGVMFTIGVQ-IVEYKTVEI 249
            G + L  A +  +S+ VLQA  L  +    +SL  +   IG +  + V  ++E+     
Sbjct: 202 KGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEHNPSAW 261

Query: 250 VWPWFVSVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTL 309
              W +++   A   ++  S+S       G  MKKRGPV  + FSP+  V   ++  F L
Sbjct: 262 RIGWDMNLLAAAYSGIVASSIS---YYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSFVL 321

Query: 310 GESINVGSLAGMFLMFTGLYFVLWAKRKESYGT 328
            E I +G + G  L+  GLY VLW K+KE+  T
Sbjct: 322 AEKIFLGGVIGAVLIVIGLYAVLWGKQKENQVT 347

BLAST of MC10g1248 vs. TAIR 10
Match: AT5G13670.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 133.3 bits (334), Expect = 3.8e-31
Identity = 98/324 (30.25%), Postives = 170/324 (52.47%), Query Frame = 0

Query: 10  LVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSKWPNKLTLKL 69
           +V +Q +YA  S++    ++ G+ P  LV +       +++P A+  ER+  P KLT K+
Sbjct: 12  IVFIQCLYALMSIVAKLALNKGMSPHVLVAYRMAVASALITPFALILERNTRP-KLTFKI 71

Query: 70  ISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRLEKVELSCIY 129
           + Q+ ++S     + Q+L+  G++LT+ +  +A+ N  P + F++A +F+LEKV +   +
Sbjct: 72  LLQIAILSLFEPVVEQNLYYSGMKLTTATFTSALCNALPAMTFIMACVFKLEKVTIERRH 131

Query: 130 SKIKILGTLLCVVGAFTMT-----IMHSASKVDSTILPLFDEQDQILDRQKIL-GCLYLF 189
           S+ K++GT++ + GA  MT     ++      +S  L       +I  +  I  G + L 
Sbjct: 132 SQAKLVGTMVAIGGAMLMTFVKGNVIELPWTSNSRGLNGHTHAMRIPKQADIARGSIMLV 191

Query: 190 TAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVM-FTIGVQIVEYKTVEIVWPWFVSV 249
            +    S  ++LQA  L  + A +SL A+  ++G++  T+   I E K + +   W ++ 
Sbjct: 192 ASCFSWSCYIILQAKILAQYKAELSLTALMCIMGMLEATVMGLIWERKNMSV---WKINP 251

Query: 250 KEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGESINVGS 309
                  + GG VSG      GWA K+RGPV VS F+P+  V   ILS F   E + VG 
Sbjct: 252 DVTLLASIYGGLVSGLAYYVIGWASKERGPVFVSAFNPLSMVLVAILSTFVFLEKVYVGR 311

Query: 310 LAGMFLMFTGLYFVLWAKRKESYG 327
           + G  ++  G+Y VLW K K+  G
Sbjct: 312 VIGSVVIVIGIYLVLWGKSKDKGG 331