MC10g1205 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g1205
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	MC10: 15207691 .. 15208242 (-)
RNA-Seq Expression	MC10g1205
Synteny	MC10g1205

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGACTCATATTTTCCACTTCCAATCACAGCCATTCTCCTCATCATTCTCATCAACCTATCAAATGCAACCATAGATGACACCCAATTCATCAAAGCCACTTGTCAAACCACACCCTATCCTGATCTCTGCTTCTCCTCACTCTCCGCGGATGCCGTTTCCGTCCACGGCAGCCTCCGCTCGATGACCATCGCTGCCCTCACCGTCGCCCTCACACACACTCGCTCCGCCTCGTCGATGGTGGCAAGTTTGGCTAAGTCGAATGCACTGGCACCGCAAGAGACTTGGGCTTTGCGTGATTGCATTGAGGAGCTTGGAGACTCTGTGGAGGAGCTCCAAAGCTCAGTGGCGGAGTTGAAAGACGGTGATGGATCTCGACCCGAGACCGATGATATTCGGACGTGGGTGAGCGCTGCTCTGACCGATGACGACACGTGCATGGAGGGACTCGTCGGAGACGGCGGCGGCGTGAAGGAGTCGGTGAGAGGACTGGTGGTGGATGTTGCTCAATTGACAAGCATTGCTCTCTCGCTTGTTAGCCATCTGAAA

mRNA sequence

ATGGAAGACTCATATTTTCCACTTCCAATCACAGCCATTCTCCTCATCATTCTCATCAACCTATCAAATGCAACCATAGATGACACCCAATTCATCAAAGCCACTTGTCAAACCACACCCTATCCTGATCTCTGCTTCTCCTCACTCTCCGCGGATGCCGTTTCCGTCCACGGCAGCCTCCGCTCGATGACCATCGCTGCCCTCACCGTCGCCCTCACACACACTCGCTCCGCCTCGTCGATGGTGGCAAGTTTGGCTAAGTCGAATGCACTGGCACCGCAAGAGACTTGGGCTTTGCGTGATTGCATTGAGGAGCTTGGAGACTCTGTGGAGGAGCTCCAAAGCTCAGTGGCGGAGTTGAAAGACGGTGATGGATCTCGACCCGAGACCGATGATATTCGGACGTGGGTGAGCGCTGCTCTGACCGATGACGACACGTGCATGGAGGGACTCGTCGGAGACGGCGGCGGCGTGAAGGAGTCGGTGAGAGGACTGGTGGTGGATGTTGCTCAATTGACAAGCATTGCTCTCTCGCTTGTTAGCCATCTGAAA

Coding sequence (CDS)

ATGGAAGACTCATATTTTCCACTTCCAATCACAGCCATTCTCCTCATCATTCTCATCAACCTATCAAATGCAACCATAGATGACACCCAATTCATCAAAGCCACTTGTCAAACCACACCCTATCCTGATCTCTGCTTCTCCTCACTCTCCGCGGATGCCGTTTCCGTCCACGGCAGCCTCCGCTCGATGACCATCGCTGCCCTCACCGTCGCCCTCACACACACTCGCTCCGCCTCGTCGATGGTGGCAAGTTTGGCTAAGTCGAATGCACTGGCACCGCAAGAGACTTGGGCTTTGCGTGATTGCATTGAGGAGCTTGGAGACTCTGTGGAGGAGCTCCAAAGCTCAGTGGCGGAGTTGAAAGACGGTGATGGATCTCGACCCGAGACCGATGATATTCGGACGTGGGTGAGCGCTGCTCTGACCGATGACGACACGTGCATGGAGGGACTCGTCGGAGACGGCGGCGGCGTGAAGGAGTCGGTGAGAGGACTGGTGGTGGATGTTGCTCAATTGACAAGCATTGCTCTCTCGCTTGTTAGCCATCTGAAA

Protein sequence

MEDSYFPLPITAILLIILINLSNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSLRSMTIAALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAELKDGDGSRPETDDIRTWVSAALTDDDTCMEGLVGDGGGVKESVRGLVVDVAQLTSIALSLVSHLK

Homology

[Close]

BLAST of MC10g1205 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 6.8e-24
Identity = 68/184 (36.96%), Postives = 108/184 (58.70%), Query Frame = 0


Query: 6   FPLPITAILLIILINLSNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSLRSMTI 65
           F L ++   +++  NL   +  DT+FIKA+C+TT YPD CF SLS+ A  +    R +  
Sbjct: 12  FVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAE 71

Query: 66  AALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAELK---D 125
            AL V++   +SA + V+ +     +  ++  A+ DC+EE+GD+V+ L +S+ ELK   +
Sbjct: 72  TALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEE 131

Query: 126 GDGSRPE---TDDIRTWVSAALTDDDTCMEGLVGD--GGGVKESVRGLVVDVAQLTSIAL 182
           GD          ++RTW SAALTD+  CM+G  G    G +K  +R  +V VA+ TS AL
Sbjct: 132 GDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEETSNAL 191

[Close]

BLAST of MC10g1205 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 7.6e-23
Identity = 72/180 (40.00%), Postives = 108/180 (60.00%), Query Frame = 0


Query: 10  ITAILLIILINLSNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSLRSMTIAALT 69
           + + LL+IL   S A  +  QFIK +C  T YP +C  SLSA A ++  + + +   AL 
Sbjct: 9   VFSALLVILAAASAAPAN--QFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTALQ 68

Query: 70  VALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAELKD-----GD 129
           V+LT T+ A + +  L K   L  ++  A+ DC+EE+ DS++ +  S  E+K+     G+
Sbjct: 69  VSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKGN 128

Query: 130 GSRPETDDIRTWVSAALTDDDTCMEGLVGDG--GGVKESVRGLVVDVAQLTSIALSLVSH 183
                  ++ TWVSAALTD+ TCM+G  G G  G +KESVR  VV VA++TS AL+LV++
Sbjct: 129 DFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNALALVNN 186

[Close]

BLAST of MC10g1205 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 9.9e-23
Identity = 67/165 (40.61%), Postives = 98/165 (59.39%), Query Frame = 0


Query: 22  SNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSLRSMTIAALTVALTHTRSASSM 81
           SN T  +  +IK +C  T Y  +C++SLS  A ++  + + + + AL + L+  +SAS  
Sbjct: 140 SNQT--NLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKF 199

Query: 82  VASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAEL-----KDGDGSRPETDDIRTW 141
           V +++    L   E  A+ DC+EE+GDSV  LQ S+ EL     KD         D+ TW
Sbjct: 200 VKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETW 259

Query: 142 VSAALTDDDTCMEGLVGDGGGVKESVRGLVVDVAQLTSIALSLVS 182
           VSAALT+DDTCM+G       VK+ VR  VV+VA+LTS AL+L++
Sbjct: 260 VSAALTNDDTCMDGFSLVKTAVKDLVRRHVVEVARLTSNALALIN 302

[Close]

BLAST of MC10g1205 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 3.5e-20
Identity = 73/185 (39.46%), Postives = 106/185 (57.30%), Query Frame = 0


Query: 10  ITAILLIILINLSNAT---IDDTQFIKATCQTTPYPDLCFSSLSADAVSV-HGSLRSMTI 69
           +  +L I ++  S+AT    +  QFI ++CQTT YP LC  +LSA A  + H + + +  
Sbjct: 9   LVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQ 68

Query: 70  AALTVALTHTRSASSMVASLAKSN-ALAPQETWALRDCIEELGDSVEELQSSVAELKDGD 129
            AL ++L   +S +  VA L K       +E  A++DCIE LG+SV+ L  SV EL    
Sbjct: 69  TALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAG 128

Query: 130 GSRPETD------DIRTWVSAALTDDDTCMEGLV--GDGGGVKESVRGLVVDVAQLTSIA 182
            +    D      +++TWVSAALTD+ TC++G      GG VK  +R  VV VAQ+TS A
Sbjct: 129 HAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTSNA 188

[Close]

BLAST of MC10g1205 vs. ExPASy Swiss-Prot
Match: Q84WE4 (Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.2e-17
Identity = 60/160 (37.50%), Postives = 94/160 (58.75%), Query Frame = 0


Query: 32  IKATCQTTPYPDLCFSSLSADAVSVHGSLRSMTIAALTVALTHTRSASSMVASLAKSNAL 91
           + ++C+   YP LC  +LS+ +     + R +  AA+ ++L+H +SA+  +A +  S   
Sbjct: 34  VHSSCEHASYPSLCVRTLSSYSGPTITNRRDLAQAAIKISLSHAQSAAKKLAVVRDSVGK 93

Query: 92  APQETWALRDCIEELGDSVEELQSSVAELKD---GDGS----RPETDDIRTWVSAALTDD 151
             QE  AL DC+E +GDSV+EL  ++  LK      GS    R +  + +TW SAALTDD
Sbjct: 94  KKQEKAALVDCVEMIGDSVDELSRTLGVLKHLRVSGGSAKEFRWQMSNAQTWASAALTDD 153

Query: 152 DTCMEGLVG-DGGGVKESVRGLVVDVAQLTSIALSLVSHL 184
           DTC++G  G D G +K  V+  +  VA++TS AL +V+ L
Sbjct: 154 DTCLDGFQGMDDGEIKTEVKQWMTKVARVTSNALYMVNQL 193

[Close]

BLAST of MC10g1205 vs. NCBI nr
Match: XP_022152813.1 (pectinesterase inhibitor 7-like [Momordica charantia])

HSP 1 Score: 343 bits (880), Expect = 1.41e-118
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 1   MEDSYFPLPITAILLIILINLSNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSL 60
           MEDSYFPLPITAILLIILINLSNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSL
Sbjct: 1   MEDSYFPLPITAILLIILINLSNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSL 60

Query: 61  RSMTIAALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAEL 120
           RSMTIAALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAEL
Sbjct: 61  RSMTIAALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAEL 120

Query: 121 KDGDGSRPETDDIRTWVSAALTDDDTCMEGLVGDGGGVKESVRGLVVDVAQLTSIALSLV 180
           KDGDGSRPETDDIRTWVSAALTDDDTCMEGLVGDGGGVKESVRGLVVDVAQLTSIALSLV
Sbjct: 121 KDGDGSRPETDDIRTWVSAALTDDDTCMEGLVGDGGGVKESVRGLVVDVAQLTSIALSLV 180

Query: 181 SHLK 184
           SHLK
Sbjct: 181 SHLK 184

[Close]

BLAST of MC10g1205 vs. NCBI nr
Match: XP_004147977.1 (21 kDa protein [Cucumis sativus])

HSP 1 Score: 234 bits (598), Expect = 1.66e-75
Identity = 132/192 (68.75%), Postives = 155/192 (80.73%), Query Frame = 0


Query: 1   MEDSYFPLPITAILLIILINLSNAT-----IDDTQFIKATCQTTPYPDLCFSSLSADAVS 60
           M+ SYFPLP+ AILLI+LIN SN       I+DTQFIK TCQ+TPYPDLC SSLS  A +
Sbjct: 1   MKSSYFPLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT 60

Query: 61  VHGSLRSMTIAALTVALTHTRSASSMVASLAKS-NALAPQETWALRDCIEELGDSVEELQ 120
           +H S   MT+AALTVALTHTRS SS + SLAKS NAL P++++ +RDCIEE GDSVEEL+
Sbjct: 61  IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK 120

Query: 121 SSVAELKDGDGSRPETDDIRTWVSAALTDDDTCMEGLVGDG--GGVKESVRGLVVDVAQL 180
            +V ELKD + SR ET+DIRTWVSAALTDDDTCM+GLVGD   G VKES++ +VV+VAQL
Sbjct: 121 MAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL 180

Query: 181 TSIALSLVSHLK 184
           TSIALSLVS LK
Sbjct: 181 TSIALSLVSLLK 192

[Close]

BLAST of MC10g1205 vs. NCBI nr
Match: XP_038876812.1 (21 kDa protein-like [Benincasa hispida])

HSP 1 Score: 234 bits (597), Expect = 2.20e-75
Identity = 132/190 (69.47%), Postives = 150/190 (78.95%), Query Frame = 0


Query: 1   MEDSYFPLPITAILLIILINLSNAT----IDDTQFIKATCQTTPYPDLCFSSLSADAVSV 60
           ME SY PLPI AILLIILIN SN      I+DTQFIK TCQ+TPYP LC SSLSADA ++
Sbjct: 1   MESSYLPLPIKAILLIILINQSNIAKSQPINDTQFIKTTCQSTPYPSLCLSSLSADAATI 60

Query: 61  HGSLRSMTIAALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSS 120
           HGS R MTIAALTVALT TRSASS++ SLAKSN L P+E + + DCIEE GDS+EEL+ +
Sbjct: 61  HGSYRLMTIAALTVALTRTRSASSVIQSLAKSNVLTPREGYVVSDCIEEFGDSIEELKMA 120

Query: 121 VAELKDGDGSRPETDDIRTWVSAALTDDDTCMEGLVGD--GGGVKESVRGLVVDVAQLTS 180
           + ELKD D  R E +DIRTWVSAALTDDDTCM+G VGD     VKES++ LVVD+ QLTS
Sbjct: 121 IVELKDNDKMRFEIEDIRTWVSAALTDDDTCMDGFVGDTMNDSVKESMKKLVVDIVQLTS 180

Query: 181 IALSLVSHLK 184
           IALSLVS LK
Sbjct: 181 IALSLVSLLK 190

[Close]

BLAST of MC10g1205 vs. NCBI nr
Match: XP_008448897.1 (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 231 bits (589), Expect = 3.87e-74
Identity = 133/192 (69.27%), Postives = 154/192 (80.21%), Query Frame = 0


Query: 1   MEDSYFPLPITAILLI-ILINLSNAT----IDDTQFIKATCQTTPYPDLCFSSLSADAVS 60
           M+ SYFPLP+ AILL+ ILIN SN       +DTQFIK TCQ+TPYPDLC SSLS  A +
Sbjct: 1   MKSSYFPLPVKAILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAA 60

Query: 61  VHGSLRSMTIAALTVALTHTRSASSMVASLAKS-NALAPQETWALRDCIEELGDSVEELQ 120
           +H S R MTIAALTVALTHTRS SS V SLAKS NAL P+E + +RDCIEE GDSVEEL+
Sbjct: 61  IHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELK 120

Query: 121 SSVAELKDGDGSRPETDDIRTWVSAALTDDDTCMEGLVGDG--GGVKESVRGLVVDVAQL 180
            ++ ELKD + +R ET+DIRTWVSAALTDDDTCM+GLVGD   G +KES++ +VVDVAQL
Sbjct: 121 MAIEELKDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQL 180

Query: 181 TSIALSLVSHLK 184
           TSIALSLVS LK
Sbjct: 181 TSIALSLVSLLK 192

[Close]

BLAST of MC10g1205 vs. NCBI nr
Match: XP_023551923.1 (21 kDa protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 219 bits (558), Expect = 1.75e-69
Identity = 128/183 (69.95%), Postives = 142/183 (77.60%), Query Frame = 0


Query: 8   LPITAILLIILINLSNAT----IDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSLRSM 67
           LPITAIL +ILIN S       I+DTQFIK TCQTTPYPDLC SSLSADA ++HGS R M
Sbjct: 6   LPITAILFMILINQSYTVATQPINDTQFIKTTCQTTPYPDLCLSSLSADASAIHGSHRLM 65

Query: 68  TIAALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAELKDG 127
           TIAAL VALTHT  ASS V SLAKSNAL P+E +AL DCIEE GDS+EEL+ +V ELK  
Sbjct: 66  TIAALAVALTHTHDASSTVTSLAKSNALTPREGYALSDCIEEFGDSIEELKMAVEELKGN 125

Query: 128 DGSRPETDDIRTWVSAALTDDDTCMEGLVGDG--GGVKESVRGLVVDVAQLTSIALSLVS 184
           D +R +  DI+TWVSAALTDD+TCM+G   D   G VKESVR LVVDVAQLTSIALSLV 
Sbjct: 126 DKTRLDIKDIQTWVSAALTDDNTCMDGFAEDAMNGSVKESVRALVVDVAQLTSIALSLVR 185

[Close]

BLAST of MC10g1205 vs. ExPASy TrEMBL
Match: A0A6J1DFW8 (pectinesterase inhibitor 7-like OS=Momordica charantia OX=3673 GN=LOC111020439 PE=4 SV=1)

HSP 1 Score: 343 bits (880), Expect = 6.82e-119
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 1   MEDSYFPLPITAILLIILINLSNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSL 60
           MEDSYFPLPITAILLIILINLSNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSL
Sbjct: 1   MEDSYFPLPITAILLIILINLSNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSL 60

Query: 61  RSMTIAALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAEL 120
           RSMTIAALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAEL
Sbjct: 61  RSMTIAALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAEL 120

Query: 121 KDGDGSRPETDDIRTWVSAALTDDDTCMEGLVGDGGGVKESVRGLVVDVAQLTSIALSLV 180
           KDGDGSRPETDDIRTWVSAALTDDDTCMEGLVGDGGGVKESVRGLVVDVAQLTSIALSLV
Sbjct: 121 KDGDGSRPETDDIRTWVSAALTDDDTCMEGLVGDGGGVKESVRGLVVDVAQLTSIALSLV 180

Query: 181 SHLK 184
           SHLK
Sbjct: 181 SHLK 184

[Close]

BLAST of MC10g1205 vs. ExPASy TrEMBL
Match: A0A0A0L1P3 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G035340 PE=4 SV=1)

HSP 1 Score: 234 bits (598), Expect = 8.02e-76
Identity = 132/192 (68.75%), Postives = 155/192 (80.73%), Query Frame = 0


Query: 1   MEDSYFPLPITAILLIILINLSNAT-----IDDTQFIKATCQTTPYPDLCFSSLSADAVS 60
           M+ SYFPLP+ AILLI+LIN SN       I+DTQFIK TCQ+TPYPDLC SSLS  A +
Sbjct: 1   MKSSYFPLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT 60

Query: 61  VHGSLRSMTIAALTVALTHTRSASSMVASLAKS-NALAPQETWALRDCIEELGDSVEELQ 120
           +H S   MT+AALTVALTHTRS SS + SLAKS NAL P++++ +RDCIEE GDSVEEL+
Sbjct: 61  IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK 120

Query: 121 SSVAELKDGDGSRPETDDIRTWVSAALTDDDTCMEGLVGDG--GGVKESVRGLVVDVAQL 180
            +V ELKD + SR ET+DIRTWVSAALTDDDTCM+GLVGD   G VKES++ +VV+VAQL
Sbjct: 121 MAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL 180

Query: 181 TSIALSLVSHLK 184
           TSIALSLVS LK
Sbjct: 181 TSIALSLVSLLK 192

[Close]

BLAST of MC10g1205 vs. ExPASy TrEMBL
Match: A0A1S3BLP3 (21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103490923 PE=4 SV=1)

HSP 1 Score: 231 bits (589), Expect = 1.87e-74
Identity = 133/192 (69.27%), Postives = 154/192 (80.21%), Query Frame = 0


Query: 1   MEDSYFPLPITAILLI-ILINLSNAT----IDDTQFIKATCQTTPYPDLCFSSLSADAVS 60
           M+ SYFPLP+ AILL+ ILIN SN       +DTQFIK TCQ+TPYPDLC SSLS  A +
Sbjct: 1   MKSSYFPLPVKAILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAA 60

Query: 61  VHGSLRSMTIAALTVALTHTRSASSMVASLAKS-NALAPQETWALRDCIEELGDSVEELQ 120
           +H S R MTIAALTVALTHTRS SS V SLAKS NAL P+E + +RDCIEE GDSVEEL+
Sbjct: 61  IHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELK 120

Query: 121 SSVAELKDGDGSRPETDDIRTWVSAALTDDDTCMEGLVGDG--GGVKESVRGLVVDVAQL 180
            ++ ELKD + +R ET+DIRTWVSAALTDDDTCM+GLVGD   G +KES++ +VVDVAQL
Sbjct: 121 MAIEELKDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQL 180

Query: 181 TSIALSLVSHLK 184
           TSIALSLVS LK
Sbjct: 181 TSIALSLVSLLK 192

[Close]

BLAST of MC10g1205 vs. ExPASy TrEMBL
Match: A0A6J1E6H9 (21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111431052 PE=4 SV=1)

HSP 1 Score: 208 bits (529), Expect = 2.16e-65
Identity = 123/190 (64.74%), Postives = 143/190 (75.26%), Query Frame = 0


Query: 1   MEDSYFPLPITAILLIILINLSNAT----IDDTQFIKATCQTTPYPDLCFSSLSADAVSV 60
           ME S   LPITAI+ +ILIN S       I+DTQFIK TCQTTPYPDLC SSLS DA ++
Sbjct: 1   MESSV--LPITAIVFVILINQSYTVATQPINDTQFIKTTCQTTPYPDLCLSSLSPDASAI 60

Query: 61  HGSLRSMTIAALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSS 120
             S R MTIAAL VALTHT  ASS V SLAKSNA+  +E +AL DCIEE GDSVEEL+ +
Sbjct: 61  RCSHRLMTIAALAVALTHTHDASSTVTSLAKSNAITTREGYALSDCIEEFGDSVEELKMA 120

Query: 121 VAELKDGDGSRPETDDIRTWVSAALTDDDTCMEGLVGDG--GGVKESVRGLVVDVAQLTS 180
           V  LK  D +R +T+DI+TW+SAALTDD+TCM+G  GD   G VKES++ +VVDVAQLTS
Sbjct: 121 VEALKGNDKTRLDTEDIQTWISAALTDDNTCMDGFAGDAMNGSVKESMKAMVVDVAQLTS 180

Query: 181 IALSLVSHLK 184
           IALSLV  LK
Sbjct: 181 IALSLVRQLK 188

[Close]

BLAST of MC10g1205 vs. ExPASy TrEMBL
Match: A0A5D3D7I0 (21 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G001400 PE=4 SV=1)

HSP 1 Score: 162 bits (410), Expect = 3.41e-48
Identity = 92/125 (73.60%), Postives = 106/125 (84.80%), Query Frame = 0


Query: 63  MTIAALTVALTHTRSASSMVASLAKS-NALAPQETWALRDCIEELGDSVEELQSSVAELK 122
           MTIAALTVALTHTRS SS V SLAKS NAL P+E + +RDCIEE GDSVEEL+ ++ ELK
Sbjct: 1   MTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELKMAIEELK 60

Query: 123 DGDGSRPETDDIRTWVSAALTDDDTCMEGLVGDG--GGVKESVRGLVVDVAQLTSIALSL 182
           D + +R ET+DIRTWVSAALTDDDTCM+GLVGD   G +KES++ +VVDVAQLTSIALSL
Sbjct: 61  DNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQLTSIALSL 120

Query: 183 VSHLK 184
           VS LK
Sbjct: 121 VSLLK 125

[Close]

BLAST of MC10g1205 vs. TAIR 10
Match: AT5G62360.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 124.4 bits (311), Expect = 9.5e-29
Identity = 78/183 (42.62%), Postives = 114/183 (62.30%), Query Frame = 0


Query: 11  TAILLIILINL-------SNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSLRSM 70
           T +++I ++ L       +  T  +T+F+K++C  T YP LCFSSLS  A  +  S + M
Sbjct: 16  TFLIIIAMLKLVHTTTTTTTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPKLM 75

Query: 71  TIAALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAELKDG 130
             AAL + L   +  S+M+  L+ S  L P+E  A+RDC+EELGD++EEL+ S+ E+   
Sbjct: 76  AHAALNITLASAKVTSAMMVRLSNSR-LKPKEVSAMRDCVEELGDTLEELRKSIGEMCQL 135

Query: 131 DGSRPET--DDIRTWVSAALTDDDTCMEGLVGD--GGGVKESVRGLVVDVAQLTSIALSL 183
            GS  E    DI+TWVSAALTD +TC +G  G+   G VK  VRG ++ +A LTS AL+L
Sbjct: 136 SGSNYEVYISDIQTWVSAALTDVNTCTDGFEGEDMDGKVKVLVRGRILVIAHLTSNALAL 195

[Close]

BLAST of MC10g1205 vs. TAIR 10
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 112.1 bits (279), Expect = 4.9e-25
Identity = 68/184 (36.96%), Postives = 108/184 (58.70%), Query Frame = 0


Query: 6   FPLPITAILLIILINLSNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSLRSMTI 65
           F L ++   +++  NL   +  DT+FIKA+C+TT YPD CF SLS+ A  +    R +  
Sbjct: 12  FVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAE 71

Query: 66  AALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAELK---D 125
            AL V++   +SA + V+ +     +  ++  A+ DC+EE+GD+V+ L +S+ ELK   +
Sbjct: 72  TALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEE 131

Query: 126 GDGSRPE---TDDIRTWVSAALTDDDTCMEGLVGD--GGGVKESVRGLVVDVAQLTSIAL 182
           GD          ++RTW SAALTD+  CM+G  G    G +K  +R  +V VA+ TS AL
Sbjct: 132 GDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEETSNAL 191

[Close]

BLAST of MC10g1205 vs. TAIR 10
Match: AT1G62760.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 108.2 bits (269), Expect = 7.0e-24
Identity = 67/165 (40.61%), Postives = 98/165 (59.39%), Query Frame = 0


Query: 22  SNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSLRSMTIAALTVALTHTRSASSM 81
           SN T  +  +IK +C  T Y  +C++SLS  A ++  + + + + AL + L+  +SAS  
Sbjct: 140 SNQT--NLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKF 199

Query: 82  VASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAEL-----KDGDGSRPETDDIRTW 141
           V +++    L   E  A+ DC+EE+GDSV  LQ S+ EL     KD         D+ TW
Sbjct: 200 VKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETW 259

Query: 142 VSAALTDDDTCMEGLVGDGGGVKESVRGLVVDVAQLTSIALSLVS 182
           VSAALT+DDTCM+G       VK+ VR  VV+VA+LTS AL+L++
Sbjct: 260 VSAALTNDDTCMDGFSLVKTAVKDLVRRHVVEVARLTSNALALIN 302

[Close]

BLAST of MC10g1205 vs. TAIR 10
Match: AT1G62770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 99.8 bits (247), Expect = 2.5e-21
Identity = 73/185 (39.46%), Postives = 106/185 (57.30%), Query Frame = 0


Query: 10  ITAILLIILINLSNAT---IDDTQFIKATCQTTPYPDLCFSSLSADAVSV-HGSLRSMTI 69
           +  +L I ++  S+AT    +  QFI ++CQTT YP LC  +LSA A  + H + + +  
Sbjct: 9   LVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQ 68

Query: 70  AALTVALTHTRSASSMVASLAKSN-ALAPQETWALRDCIEELGDSVEELQSSVAELKDGD 129
            AL ++L   +S +  VA L K       +E  A++DCIE LG+SV+ L  SV EL    
Sbjct: 69  TALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAG 128

Query: 130 GSRPETD------DIRTWVSAALTDDDTCMEGLV--GDGGGVKESVRGLVVDVAQLTSIA 182
            +    D      +++TWVSAALTD+ TC++G      GG VK  +R  VV VAQ+TS A
Sbjct: 129 HAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTSNA 188

[Close]

BLAST of MC10g1205 vs. TAIR 10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 98.6 bits (244), Expect = 5.6e-21
Identity = 61/159 (38.36%), Postives = 91/159 (57.23%), Query Frame = 0


Query: 31  FIKATCQTTPYPDLCFSSLSADAVSVHGSLRSMTIAALTVALTHTRSASSMVASLAKSNA 90
           FI+++C+TT YP LC  SLS  A  +  S + +   A+ V L+  +S    V+ L +   
Sbjct: 38  FIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLAETAIAVTLSRAQSTKLFVSRLTRMKG 97

Query: 91  LAPQETWALRDCIEELGDSVEELQSSVAEL------KDGDGSRPETDDIRTWVSAALTDD 150
           L  +E  A++DC+EE+ D+V+ L  SV EL      KD D       + +TW SAALTD+
Sbjct: 98  LKKREVEAIKDCVEEMNDTVDRLTKSVQELKLCGSAKDQDQFAYHMSNAQTWTSAALTDE 157

Query: 151 DTCMEGLVGD--GGGVKESVRGLVVDVAQLTSIALSLVS 182
           +TC +G  G    G +K SVR  +++V   TS ALSL++
Sbjct: 158 NTCSDGFSGRVMDGRIKNSVRARIMNVGHETSNALSLIN 196

The following BLAST results are available for this feature:

BLAST of MC10g1205 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SB37	6.8e-24	36.96	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
P17407	7.6e-23	40.00	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9SI74	9.9e-23	40.61	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]
Q9SI72	3.5e-20	39.46	Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1	[more]
Q84WE4	1.2e-17	37.50	Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1	[more]

BLAST of MC10g1205 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152813.1	1.41e-118	100.00	pectinesterase inhibitor 7-like [Momordica charantia]	[more]
XP_004147977.1	1.66e-75	68.75	21 kDa protein [Cucumis sativus]	[more]
XP_038876812.1	2.20e-75	69.47	21 kDa protein-like [Benincasa hispida]	[more]
XP_008448897.1	3.87e-74	69.27	PREDICTED: 21 kDa protein-like [Cucumis melo]	[more]
XP_023551923.1	1.75e-69	69.95	21 kDa protein-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC10g1205 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DFW8	6.82e-119	100.00	pectinesterase inhibitor 7-like OS=Momordica charantia OX=3673 GN=LOC111020439 P...	[more]
A0A0A0L1P3	8.02e-76	68.75	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G035340 PE=4 S...	[more]
A0A1S3BLP3	1.87e-74	69.27	21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103490923 PE=4 SV=1	[more]
A0A6J1E6H9	2.16e-65	64.74	21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111431052 PE=4 SV=1	[more]
A0A5D3D7I0	3.41e-48	73.60	21 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G...	[more]

BLAST of MC10g1205 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G62360.1	9.5e-29	42.62	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25260.1	4.9e-25	36.96	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62760.1	7.0e-24	40.61	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62770.1	2.5e-21	39.46	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62350.1	5.6e-21	38.36	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 103..123
None	No IPR available	PANTHER	PTHR31080:SF161	21 KDA PROTEIN-LIKE	coord: 23..183
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 23..183
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 34..183 e-value: 1.64056E-43 score: 140.266
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 27..179 e-value: 7.7E-47 score: 171.6
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 10..183 e-value: 2.1E-34 score: 116.3
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 31..179 e-value: 4.9E-36 score: 124.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 26..184 e-value: 1.2E-40 score: 141.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 30..183

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g1205.1	MC10g1205.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity